LOCUS       DAZUMB010000001        69979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000001 DAZUMB010000000
VERSION     DAZUMB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.001"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1535)
                     /locus_tag="ACG80L_000001"
     CDS             complement(12..1535)
                     /locus_tag="ACG80L_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5022298.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ACG80L_000002"
     CDS             1787..2968
                     /locus_tag="ACG80L_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5022299.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACG80L_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACG80L_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS5022300.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ACG80L_000004"
     CDS             complement(5053..7764)
                     /locus_tag="ACG80L_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS5022301.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ACG80L_000005"
     CDS             complement(7774..8475)
                     /locus_tag="ACG80L_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022302.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ACG80L_000006"
     CDS             8661..9260
                     /locus_tag="ACG80L_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5022303.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ACG80L_000007"
     CDS             9341..10354
                     /locus_tag="ACG80L_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS5022304.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ACG80L_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ACG80L_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS5022305.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ACG80L_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ACG80L_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS5022306.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ACG80L_000010"
     CDS             11802..13175
                     /locus_tag="ACG80L_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5022307.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ACG80L_000011"
     CDS             13532..15676
                     /locus_tag="ACG80L_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5022308.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ACG80L_000012"
     CDS             complement(15728..16768)
                     /locus_tag="ACG80L_000012"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5022309.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ACG80L_000013"
     CDS             complement(17045..18847)
                     /locus_tag="ACG80L_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022310.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ACG80L_000014"
     CDS             complement(18861..19613)
                     /locus_tag="ACG80L_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022311.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ACG80L_000015"
     CDS             complement(19622..19984)
                     /locus_tag="ACG80L_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5022312.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ACG80L_000016"
     CDS             complement(20160..21032)
                     /locus_tag="ACG80L_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5022313.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ACG80L_000017"
     CDS             complement(21058..21570)
                     /locus_tag="ACG80L_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5022314.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ACG80L_000018"
     CDS             complement(21708..22499)
                     /locus_tag="ACG80L_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS5022315.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ACG80L_000019"
     CDS             complement(22554..23162)
                     /locus_tag="ACG80L_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS5022316.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ACG80L_000020"
     CDS             complement(23155..23472)
                     /locus_tag="ACG80L_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5022317.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ACG80L_000021"
     CDS             complement(23628..24356)
                     /locus_tag="ACG80L_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS5022318.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ACG80L_000022"
     CDS             24515..25396
                     /locus_tag="ACG80L_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5022319.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ACG80L_000023"
     CDS             complement(25633..26676)
                     /locus_tag="ACG80L_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5022320.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ACG80L_000024"
     CDS             complement(26679..27347)
                     /locus_tag="ACG80L_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5022321.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ACG80L_000025"
     CDS             27489..29825
                     /locus_tag="ACG80L_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS5022322.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ACG80L_000026"
     CDS             complement(29940..30251)
                     /locus_tag="ACG80L_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022323.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ACG80L_000027"
     CDS             30432..31907
                     /locus_tag="ACG80L_000027"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS5022324.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ACG80L_000028"
     CDS             31954..32538
                     /locus_tag="ACG80L_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS5022325.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ACG80L_000029"
     CDS             32540..32959
                     /locus_tag="ACG80L_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS5022326.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ACG80L_000030"
     CDS             complement(33240..33524)
                     /locus_tag="ACG80L_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS5022327.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ACG80L_000031"
     CDS             complement(33876..34586)
                     /locus_tag="ACG80L_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS5022328.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ACG80L_000032"
     CDS             complement(34583..36055)
                     /locus_tag="ACG80L_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5022329.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ACG80L_000033"
     CDS             36193..36666
                     /locus_tag="ACG80L_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5022330.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ACG80L_000034"
     CDS             complement(36819..37370)
                     /locus_tag="ACG80L_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5022331.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ACG80L_000035"
     CDS             37475..38005
                     /locus_tag="ACG80L_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS5022332.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ACG80L_000036"
     CDS             complement(38488..38931)
                     /locus_tag="ACG80L_000036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5022333.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ACG80L_000037"
     CDS             complement(38947..40107)
                     /locus_tag="ACG80L_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5022334.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ACG80L_000038"
     CDS             40614..42314
                     /locus_tag="ACG80L_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022335.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ACG80L_000039"
     CDS             complement(42416..43249)
                     /locus_tag="ACG80L_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS5022336.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ACG80L_000040"
     CDS             complement(43553..45718)
                     /locus_tag="ACG80L_000040"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS5022337.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ACG80L_000041"
     CDS             45978..48272
                     /locus_tag="ACG80L_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS5022338.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ACG80L_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ACG80L_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5022339.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ACG80L_000043"
     CDS             48923..49930
                     /locus_tag="ACG80L_000043"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5022340.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ACG80L_000044"
     CDS             49952..50971
                     /locus_tag="ACG80L_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5022341.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACG80L_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACG80L_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5022342.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACG80L_000046"
     CDS             complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACG80L_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS5022343.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACG80L_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACG80L_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5022344.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACG80L_000048"
     CDS             complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACG80L_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS5022345.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ACG80L_000049"
     CDS             58397..59698
                     /locus_tag="ACG80L_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5022346.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ACG80L_000050"
     CDS             complement(59767..60366)
                     /locus_tag="ACG80L_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5022347.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="ACG80L_000051"
     CDS             60620..61501
                     /locus_tag="ACG80L_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5022348.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="ACG80L_000052"
     CDS             61514..63124
                     /locus_tag="ACG80L_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022349.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="ACG80L_000053"
     CDS             complement(63201..63860)
                     /locus_tag="ACG80L_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5022350.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="ACG80L_000054"
     CDS             complement(64143..64808)
                     /locus_tag="ACG80L_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS5022351.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ACG80L_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ACG80L_000055"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS5022352.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ACG80L_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ACG80L_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS5022353.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="ACG80L_000057"
     CDS             complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="ACG80L_000057"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS5022354.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="ACG80L_000058"
     CDS             67122..67442
                     /locus_tag="ACG80L_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS5022355.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68411)
                     /locus_tag="ACG80L_000059"
     CDS             complement(67554..68411)
                     /locus_tag="ACG80L_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5022356.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="ACG80L_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="ACG80L_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT        20798 a        14355 c        12621 g        22205 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttca
//
LOCUS       DAZUMB010000002        60875 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000002 DAZUMB010000000
VERSION     DAZUMB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.002"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="ACG80L_000061"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="ACG80L_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS5022357.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="ACG80L_000062"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="ACG80L_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS5022358.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="ACG80L_000063"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="ACG80L_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS5022359.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4744..5628
                     /gene="ftsX"
                     /locus_tag="ACG80L_000064"
     CDS             4744..5628
                     /gene="ftsX"
                     /locus_tag="ACG80L_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS5022360.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="ACG80L_000065"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="ACG80L_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5022361.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="ACG80L_000066"
     CDS             7565..8422
                     /locus_tag="ACG80L_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS5022362.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="ACG80L_000067"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="ACG80L_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS5022363.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="ACG80L_000068"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="ACG80L_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS5022364.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="ACG80L_000069"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="ACG80L_000069"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5022365.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /gene="pstB"
                     /locus_tag="ACG80L_000070"
     CDS             11178..11936
                     /gene="pstB"
                     /locus_tag="ACG80L_000070"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5022366.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="ACG80L_000071"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="ACG80L_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS5022367.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13203..13895
                     /locus_tag="ACG80L_000072"
     CDS             13203..13895
                     /locus_tag="ACG80L_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5022368.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13892..14659
                     /locus_tag="ACG80L_000073"
     CDS             13892..14659
                     /locus_tag="ACG80L_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5022369.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="ACG80L_000074"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="ACG80L_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS5022370.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="ACG80L_000075"
     CDS             16624..16944
                     /locus_tag="ACG80L_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS5022371.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="ACG80L_000076"
     CDS             16944..17294
                     /locus_tag="ACG80L_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS5022372.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /gene="hprK"
                     /locus_tag="ACG80L_000077"
     CDS             17461..18399
                     /gene="hprK"
                     /locus_tag="ACG80L_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS5022373.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /gene="lgt"
                     /locus_tag="ACG80L_000078"
     CDS             18414..19244
                     /gene="lgt"
                     /locus_tag="ACG80L_000078"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5022374.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="ACG80L_000079"
     CDS             19288..20313
                     /locus_tag="ACG80L_000079"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5022375.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="ACG80L_000080"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="ACG80L_000080"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5022376.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="ACG80L_000081"
     CDS             21391..21831
                     /locus_tag="ACG80L_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS5022377.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="ACG80L_000082"
     CDS             21834..22436
                     /locus_tag="ACG80L_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5022378.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="ACG80L_000083"
     CDS             complement(22495..23601)
                     /locus_tag="ACG80L_000083"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS5022379.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="ACG80L_000084"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="ACG80L_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS5022380.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="ACG80L_000085"
     CDS             complement(25149..27494)
                     /locus_tag="ACG80L_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS5022381.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="ACG80L_000086"
     CDS             27773..28675
                     /locus_tag="ACG80L_000086"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS5022382.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="ACG80L_000087"
     CDS             28888..30663
                     /locus_tag="ACG80L_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS5022383.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="ACG80L_000088"
     CDS             complement(30729..32315)
                     /locus_tag="ACG80L_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS5022384.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="ACG80L_000089"
     CDS             32566..32790
                     /locus_tag="ACG80L_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS5022385.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="ACG80L_000090"
     CDS             32932..34683
                     /locus_tag="ACG80L_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS5022386.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="ACG80L_000091"
     CDS             34683..36467
                     /locus_tag="ACG80L_000091"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022387.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /gene="licT"
                     /locus_tag="ACG80L_000092"
     CDS             36793..37644
                     /gene="licT"
                     /locus_tag="ACG80L_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5022388.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37737..39623
                     /locus_tag="ACG80L_000093"
                     /pseudo
     CDS             37737..39623
                     /locus_tag="ACG80L_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(39663..40565)
                     /locus_tag="ACG80L_000094"
     CDS             complement(39663..40565)
                     /locus_tag="ACG80L_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5022389.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="ACG80L_000095"
     CDS             40796..42199
                     /locus_tag="ACG80L_000095"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5022390.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="ACG80L_000096"
     CDS             42199..43632
                     /locus_tag="ACG80L_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5022391.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="ACG80L_000097"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="ACG80L_000097"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS5022392.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="ACG80L_000098"
     CDS             44917..46644
                     /locus_tag="ACG80L_000098"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS5022393.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="ACG80L_000099"
     CDS             46659..47009
                     /locus_tag="ACG80L_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5022394.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="ACG80L_000100"
     CDS             complement(47101..47961)
                     /locus_tag="ACG80L_000100"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS5022395.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="ACG80L_000101"
     CDS             48053..48481
                     /locus_tag="ACG80L_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5022396.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /gene="rpsU"
                     /locus_tag="ACG80L_000102"
     CDS             48643..48819
                     /gene="rpsU"
                     /locus_tag="ACG80L_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS5022397.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="ACG80L_000103"
     CDS             48846..49292
                     /locus_tag="ACG80L_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS5022398.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="ACG80L_000104"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="ACG80L_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5022399.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="ACG80L_000105"
     CDS             51531..52502
                     /locus_tag="ACG80L_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS5022400.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="ACG80L_000106"
     CDS             52526..54718
                     /locus_tag="ACG80L_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS5022401.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="ACG80L_000107"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="ACG80L_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS5022402.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="ACG80L_000108"
     CDS             55195..55590
                     /locus_tag="ACG80L_000108"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5022403.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="ACG80L_000109"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="ACG80L_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS5022404.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="ACG80L_000110"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="ACG80L_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS5022405.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="ACG80L_000111"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="ACG80L_000111"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS5022406.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /gene="glyS"
                     /locus_tag="ACG80L_000112"
     CDS             58762..60837
                     /gene="glyS"
                     /locus_tag="ACG80L_000112"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS5022407.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DAZUMB010000003        49608 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000003 DAZUMB010000000
VERSION     DAZUMB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.003"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..986
                     /locus_tag="ACG80L_000113"
     CDS             <1..986
                     /locus_tag="ACG80L_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5022408.1"
                     /translation="LEKNHIPVFIIFGNHDFYQKERYWFSFPKNVHLFTSETVETKKI
                     TIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYHIGLYHGEPGVSQKGNYAPFQP
                     SKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGHTKKEQASTSILLVELSKGSCQ
                     IHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSKVEDGFSLIEIKLKDYDHLNTD
                     VLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTFYTKTPLAASAKLMEQLFQYYQ
                     TPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAKQLLNQDFLFEEDQE"
     gene            983..3703
                     /locus_tag="ACG80L_000114"
     CDS             983..3703
                     /locus_tag="ACG80L_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5022409.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3687..4631
                     /locus_tag="ACG80L_000115"
     CDS             3687..4631
                     /locus_tag="ACG80L_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5022410.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(4700..5710)
                     /locus_tag="ACG80L_000116"
     CDS             complement(4700..5710)
                     /locus_tag="ACG80L_000116"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5022411.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(5890..6234)
                     /locus_tag="ACG80L_000117"
     CDS             complement(5890..6234)
                     /locus_tag="ACG80L_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5022412.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6227..6661)
                     /locus_tag="ACG80L_000118"
     CDS             complement(6227..6661)
                     /locus_tag="ACG80L_000118"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS5022413.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            6799..7536
                     /locus_tag="ACG80L_000119"
     CDS             6799..7536
                     /locus_tag="ACG80L_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022414.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7529..8740
                     /locus_tag="ACG80L_000120"
     CDS             7529..8740
                     /locus_tag="ACG80L_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022415.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            8944..9726
                     /locus_tag="ACG80L_000121"
     CDS             8944..9726
                     /locus_tag="ACG80L_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS5022416.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            9796..10458
                     /gene="trmB"
                     /locus_tag="ACG80L_000122"
     CDS             9796..10458
                     /gene="trmB"
                     /locus_tag="ACG80L_000122"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS5022417.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10556..12310)
                     /gene="ade"
                     /locus_tag="ACG80L_000123"
     CDS             complement(10556..12310)
                     /gene="ade"
                     /locus_tag="ACG80L_000123"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS5022418.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12442..13371
                     /gene="comGA"
                     /locus_tag="ACG80L_000124"
     CDS             12442..13371
                     /gene="comGA"
                     /locus_tag="ACG80L_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS5022419.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13328..14377
                     /gene="comGB"
                     /locus_tag="ACG80L_000125"
     CDS             13328..14377
                     /gene="comGB"
                     /locus_tag="ACG80L_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS5022420.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14374..14652
                     /gene="comGC"
                     /locus_tag="ACG80L_000126"
     CDS             14374..14652
                     /gene="comGC"
                     /locus_tag="ACG80L_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS5022421.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            14627..15097
                     /gene="comGD"
                     /locus_tag="ACG80L_000127"
     CDS             14627..15097
                     /gene="comGD"
                     /locus_tag="ACG80L_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS5022422.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15069..15416
                     /locus_tag="ACG80L_000128"
     CDS             15069..15416
                     /locus_tag="ACG80L_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022423.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            15379..15804
                     /gene="comGF"
                     /locus_tag="ACG80L_000129"
     CDS             15379..15804
                     /gene="comGF"
                     /locus_tag="ACG80L_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS5022424.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            15776..16135
                     /gene="comGG"
                     /locus_tag="ACG80L_000130"
     CDS             15776..16135
                     /gene="comGG"
                     /locus_tag="ACG80L_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS5022425.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16307..17314
                     /locus_tag="ACG80L_000131"
     CDS             16307..17314
                     /locus_tag="ACG80L_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5022426.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17339..18523
                     /locus_tag="ACG80L_000132"
     CDS             17339..18523
                     /locus_tag="ACG80L_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS5022427.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(18960..20543)
                     /gene="abc-f"
                     /locus_tag="ACG80L_000133"
     CDS             complement(18960..20543)
                     /gene="abc-f"
                     /locus_tag="ACG80L_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS5022428.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20648..20776)
                     /locus_tag="ACG80L_000134"
     CDS             complement(20648..20776)
                     /locus_tag="ACG80L_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022429.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(21121..21264)
                     /locus_tag="ACG80L_000135"
     CDS             complement(21121..21264)
                     /locus_tag="ACG80L_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS5022430.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21486..21965)
                     /locus_tag="ACG80L_000136"
     CDS             complement(21486..21965)
                     /locus_tag="ACG80L_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5022431.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22038..22322)
                     /locus_tag="ACG80L_000137"
     CDS             complement(22038..22322)
                     /locus_tag="ACG80L_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022432.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22400..22594)
                     /gene="ssrS"
                     /locus_tag="ACG80L_000138"
     ncRNA           complement(22400..22594)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG80L_000138"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22666..23952)
                     /locus_tag="ACG80L_000139"
     CDS             complement(22666..23952)
                     /locus_tag="ACG80L_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS5022433.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24090..24761
                     /locus_tag="ACG80L_000140"
     CDS             24090..24761
                     /locus_tag="ACG80L_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS5022434.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            24727..25215
                     /locus_tag="ACG80L_000141"
     CDS             24727..25215
                     /locus_tag="ACG80L_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS5022435.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25228..26175
                     /gene="prmA"
                     /locus_tag="ACG80L_000142"
     CDS             25228..26175
                     /gene="prmA"
                     /locus_tag="ACG80L_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS5022436.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26176..26940
                     /locus_tag="ACG80L_000143"
     CDS             26176..26940
                     /locus_tag="ACG80L_000143"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS5022437.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27173..29386
                     /locus_tag="ACG80L_000144"
     CDS             27173..29386
                     /locus_tag="ACG80L_000144"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS5022438.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29397..29843
                     /gene="dtd"
                     /locus_tag="ACG80L_000145"
     CDS             29397..29843
                     /gene="dtd"
                     /locus_tag="ACG80L_000145"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS5022439.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30014..31273
                     /locus_tag="ACG80L_000146"
     CDS             30014..31273
                     /locus_tag="ACG80L_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS5022440.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31362..32309)
                     /locus_tag="ACG80L_000147"
     CDS             complement(31362..32309)
                     /locus_tag="ACG80L_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5022441.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            32703..33995
                     /gene="hisS"
                     /locus_tag="ACG80L_000148"
     CDS             32703..33995
                     /gene="hisS"
                     /locus_tag="ACG80L_000148"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS5022442.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34010..35779
                     /gene="aspS"
                     /locus_tag="ACG80L_000149"
     CDS             34010..35779
                     /gene="aspS"
                     /locus_tag="ACG80L_000149"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS5022443.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            35888..36763
                     /locus_tag="ACG80L_000150"
     CDS             35888..36763
                     /locus_tag="ACG80L_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5022444.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37029..38912
                     /locus_tag="ACG80L_000151"
     CDS             37029..38912
                     /locus_tag="ACG80L_000151"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5022445.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(38987..39271)
                     /locus_tag="ACG80L_000152"
     CDS             complement(38987..39271)
                     /locus_tag="ACG80L_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5022446.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39261..39995)
                     /locus_tag="ACG80L_000153"
     CDS             complement(39261..39995)
                     /locus_tag="ACG80L_000153"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS5022447.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40124..40747
                     /locus_tag="ACG80L_000154"
     CDS             40124..40747
                     /locus_tag="ACG80L_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS5022448.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            40939..42060
                     /locus_tag="ACG80L_000155"
     CDS             40939..42060
                     /locus_tag="ACG80L_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5022449.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42067..45195
                     /locus_tag="ACG80L_000156"
     CDS             42067..45195
                     /locus_tag="ACG80L_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFS5022450.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45214..48408
                     /locus_tag="ACG80L_000157"
     CDS             45214..48408
                     /locus_tag="ACG80L_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS5022451.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48554..49261)
                     /locus_tag="ACG80L_000158"
     CDS             complement(48554..49261)
                     /locus_tag="ACG80L_000158"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5022452.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16150 a         8743 c        10365 g        14350 t
ORIGIN      
        1 ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa
       61 aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag
      121 agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc
      181 gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt
      241 accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt
      301 tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg
      361 tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata
      421 ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc
      481 aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga
      541 aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg
      601 atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt
      661 tggaacggat acaatcagga gagctattag actatttgtt agaggaattc tcagatagga
      721 taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc
      781 cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag
      841 atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg
      901 aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt
      961 ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa
     1021 atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga
     1081 agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa
     1141 aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg
     1201 gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg
     1261 aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat
     1321 cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat
     1381 ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac
     1441 gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt
     1501 caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca
     1561 agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga
     1621 acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca
     1681 attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct
     1741 caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt
     1801 ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa
     1861 gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc aggagcagga
     1921 actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag
     1981 attacaaatt gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca
     2041 ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct
     2101 ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc
     2161 taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc
     2221 attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat
     2281 ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct
     2341 tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa
     2401 aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga
     2461 tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa
     2521 acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga
     2581 tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg
     2641 cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga
     2701 ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa
     2761 aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta
     2821 tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg
     2881 gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc
     2941 tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc
     3001 ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact
     3061 gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa
     3121 tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact
     3181 ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc
     3241 acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt
     3301 accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca
     3361 gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat
     3421 ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct
     3481 ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta
     3541 tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca
     3601 aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat
     3661 cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc
     3721 agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa
     3781 aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg
     3841 cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa
     3901 gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag
     3961 atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg
     4021 aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc
     4081 gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc
     4141 atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat
     4201 caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc
     4261 atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag
     4321 gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt
     4381 tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt ttagcacacc
     4441 atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac
     4501 atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag
     4561 aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa
     4621 aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct
     4681 tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt gaagattctt
     4741 ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt tcacttgagt
     4801 ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag gcatcatctt
     4861 tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg aatgcattgt
     4921 cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta tccatgtcat
     4981 tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca taagaagtag
     5041 gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa gctgcttctt
     5101 ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg ccatcttctt
     5161 ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct ccatcatccg
     5221 ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt tgtgcttcta
     5281 cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata tctacgtgag
     5341 ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa gtatctttcg
     5401 tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc gcttgtttgt
     5461 cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta ttgaatactt
     5521 tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt gcttcatcat
     5581 agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta ttagtatcgc
     5641 ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa ataattgatt
     5701 tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata ttaactatac
     5761 catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt caaaatttca
     5821 tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct gttgagaaaa
     5881 taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt tttctacttg
     5941 ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga tatctttttt
     6001 gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt cgatcaatgc
     6061 gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg cttcttccaa
     6121 ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata acagacctcc
     6181 gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa tcatcgcagg
     6241 acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca taactatctt
     6301 gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg actaaatgga
     6361 tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag atattaagac
     6421 ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca cgcgaaaaaa
     6481 cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt ttaggcacaa
     6541 ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc ttgtcgtctt
     6601 catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata cagttttcca
     6661 tataaaacat cctttctata aagaataaat acttcagctt aactttacca taagttaaag
     6721 ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata aataaaaata
     6781 aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg gtacggacat
     6841 ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt agggttgatt
     6901 ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct gctggaagct
     6961 tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga agaatatcgc
     7021 aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac attgagagaa
     7081 catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat gaaaagagca
     7141 gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc agctaatttt
     7201 tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga gccttcttta
     7261 tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc attgcttgaa
     7321 ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca tattttagct
     7381 actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat acgagcagac
     7441 ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt ggacgaaatc
     7501 tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac gtttagcaag
     7561 acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt ttgttttagt
     7621 ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga aaaccctgca
     7681 gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct gtttattcgt
     7741 gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc ctaaagaaaa
     7801 agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc cgttgatctt
     7861 tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg agcaatcctt
     7921 ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc atctgcaaat
     7981 caagagagca gaattgtttc gactagatca acaagttatt cgttattgga aattaggctg
     8041 gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat gggcaggaat
     8101 agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc aacaagaaaa
     8161 aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc agatctatca
     8221 atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa gaagacgcta
     8281 tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc tttacttgtt
     8341 tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat tagtaattat
     8401 aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta tcggtttctt
     8461 atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc gctatatggt
     8521 tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc agtatctact
     8581 cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag tcgtattaga
     8641 tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc tctttgttaa
     8701 actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct gtgtagggta
     8761 aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct attttttttg
     8821 agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt gcttgacgtg
     8881 tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag gacgggagaa
     8941 tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa aggcgacaca
     9001 ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg aaacacgact
     9061 ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg gacaaaacga
     9121 acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt gctgactcct
     9181 aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca tcattcaaat
     9241 tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga gaagatgctg
     9301 caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct gcaaattgtc
     9361 catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc agtagtacac
     9421 ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct agtcgattgg
     9481 gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg aaattatgtg
     9541 ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac tgacgaaatg
     9601 cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac cagatattgc
     9661 aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg aattttcagt
     9721 ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat tagaggaaag
     9781 gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat gttagcggct
     9841 cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa tgaattgttt
     9901 gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt catcactgga
     9961 atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt cagtgttgtt
    10021 tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact tctacatgtg
    10081 gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat ctatctgaat
    10141 ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata taagactttt
    10201 cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa aacagataat
    10261 caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat cctcaaacag
    10321 gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga gtatgaagaa
    10381 aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca agataagacc
    10441 aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat atccataaat
    10501 aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat ttgatttaaa
    10561 atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga gaaaatgaaa
    10621 ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag aaagaaagtg
    10681 tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt attaattcat
    10741 tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct ataggaagag
    10801 aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct ttttgaatca
    10861 aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact agattatgcg
    10921 agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct ttcactatgc
    10981 ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt aattggtctt
    11041 tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc aaatctttag
    11101 tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa agaagcagct
    11161 ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac gtatatgaat
    11221 ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact aatgttccct
    11281 tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag aaatcagctt
    11341 gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct gcattcaatg
    11401 ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga cggacgatat
    11461 gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg acaaagaagc
    11521 atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct tttgcaacgg
    11581 tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct tcttttgcag
    11641 ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac tcttccatgc
    11701 cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat tggatctttt
    11761 ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca gctaagccta
    11821 aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt gcaccattgg
    11881 tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc atgagtgttt
    11941 gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt cctatttcat
    12001 ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa aattcgcttg
    12061 gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc actagatatt
    12121 gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg atcattcctt
    12181 cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg atcttagcat
    12241 gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat ttccttaatg
    12301 ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata aattcggctt
    12361 ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa aagacgtatg
    12421 ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat gataagtgaa
    12481 agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt attttacatc
    12541 tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc agatggacag
    12601 caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa aagaaaagct
    12661 cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg gttgtcttct
    12721 gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg tacttttgaa
    12781 acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa aaccgtcaga
    12841 cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct aatgtatcgg
    12901 ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga gatagaagaa
    12961 ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga tacacttttg
    13021 aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag agatgaatca
    13081 actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc aaccgtacat
    13141 gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa aaaagaagag
    13201 ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc caatcaccat
    13261 ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa atcatgggag
    13321 gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta aaggattaac
    13381 aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat ctggttttag
    13441 tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg ttattttggc
    13501 aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt cgcaaatagg
    13561 atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga atctgccgac
    13621 aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca atgatttgaa
    13681 aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct tgtttagtat
    13741 gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag agcattgggg
    13801 tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag cggttactct
    13861 attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc agagtgaatg
    13921 gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg cttatttgtc
    13981 actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca tagtctgctt
    14041 aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga ttcaaggatt
    14101 ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat cagaagaatt
    14161 cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac tgatttttta
    14221 cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc attttattca
    14281 gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg ctgttttatt
    14341 acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat atcttattat
    14401 agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt attagtgttg
    14461 ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag tcgtgaagcg
    14521 atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa tggaagagtt
    14581 cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc agaaatctat
    14641 cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat cactcttact
    14701 gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg catggaagaa
    14761 tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt ctacgcaaaa
    14821 aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag agaatcaaat
    14881 catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata ttcctgatga
    14941 gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga atgaaagttc
    15001 actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt atcaattcca
    15061 gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt ttgttggagt
    15121 cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt ggccagctgt
    15181 ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga atgatttatg
    15241 aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa gaaatgatcc
    15301 catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt gtcaatatgg
    15361 agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct gattgaatgt
    15421 ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt gattcagcaa
    15481 accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct aatattttta
    15541 gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa tcagcaactg
    15601 cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat gatacgtaaa
    15661 agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct tcaattattg
    15721 gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga tggatatgcc
    15781 atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat tttctgtctt
    15841 gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct accgtttgtc
    15901 aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga agcagatggc
    15961 tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact acacaactgg
    16021 atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag tctcaccgtt
    16081 tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag aatgatggaa
    16141 tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa aatgtttgcc
    16201 gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc gatagaatta
    16261 aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt taccagaaaa
    16321 aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga atgcattggg
    16381 aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg attatcaagt
    16441 acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag ctctttatga
    16501 agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc aattactttt
    16561 attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc ctgatagtat
    16621 cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag caccggttaa
    16681 gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt taaacctatc
    16741 gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat tgttggctgt
    16801 cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc aagacgggtt
    16861 gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa tcggttatta
    16921 tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc atagttatgc
    16981 acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt ttggtttgtt
    17041 tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa aatggtttaa
    17101 agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc gtaataaaca
    17161 atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac aagtaaaaga
    17221 agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg aatttttcaa
    17281 cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg agagtcatat
    17341 gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat tatatcaaat
    17401 gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac aagattctat
    17461 ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata tcgaaaacca
    17521 cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt taagctctta
    17581 tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt atggggattc
    17641 agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat ttgctccttt
    17701 gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac ctgacatttt
    17761 gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa attatttata
    17821 tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct tccatggcac
    17881 aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga tcgaagaact
    17941 aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg atggaggtaa
    18001 atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg gtacacgttc
    18061 aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga ttgatattga
    18121 tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag gattgtctag
    18181 tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag cttatgacat
    18241 tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga atggtgttga
    18301 tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta gtgaaatcat
    18361 caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat tgcgtggtga
    18421 agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc caacagatga
    18481 ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag cattcgttag
    18541 gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt gcggagaaga
    18601 ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt cttagggaat
    18661 tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg attttcagga
    18721 aaatcccaat attttatttt cattcatctt tataagggac attacatatg taaaaagaaa
    18781 aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta tgtttagcta
    18841 gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc cacctgtact
    18901 ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga aaaaacgtcc
    18961 tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat aacgagtgct
    19021 tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag cgttccttga
    19081 tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg attggttggt
    19141 tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc caaacggatt
    19201 ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata aaagccactt
    19261 ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc gtaagccagc
    19321 aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat ttgcaagcta
    19381 ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa tttgagtaag
    19441 gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg atagactgca
    19501 aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt ttgaatgatg
    19561 aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt tttcggggga
    19621 ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt ggcttttttg
    19681 aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat catgactttc
    19741 aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc caagcgagct
    19801 tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc accttcttcc
    19861 agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga aacgaccaga
    19921 accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt aagatctaaa
    19981 tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat tttggctaaa
    20041 gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg acagtgtgga
    20101 atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc gcctagttct
    20161 tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt tgtatctggt
    20221 tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat ctcagcggca
    20281 gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg ttcgaccgtc
    20341 ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc tctgccggtc
    20401 aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc aattttttca
    20461 ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat tgttttttta
    20521 atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa aggccatggt
    20581 tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct tgagatggcc
    20641 attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca gcaactgtaa
    20701 aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta cttgttattt
    20761 tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc agtgtacctg
    20821 aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga tgtgtaattc
    20881 tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca tcattgtatt
    20941 ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac tttgtctaca
    21001 tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct ctttggaaat
    21061 ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg tagcctttca
    21121 tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc cgaacccgaa
    21181 ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat attttttcgt
    21241 atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat gcttaggtcg
    21301 ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat tttacttgaa
    21361 ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg cttgccgcgt
    21421 cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct
    21481 acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc agttcggaca
    21541 acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc aataaataaa
    21601 cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg attatcttct
    21661 gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga ggttacatta
    21721 ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt tgccatttca
    21781 gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca
    21841 atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac cgccttttta
    21901 aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt gtattgttgt
    21961 aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta gaacacttta
    22021 gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa tcggatactg
    22081 aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa gcgttgattg
    22141 aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc gtaaaattgt
    22201 aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc ctgtttatta
    22261 aataaataat agctaacgaa taataatact aaagtggcga taatcattaa aatcctaatc
    22321 atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg
    22381 ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg tagtattgat
    22441 cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa atgataaggc
    22501 atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt tcggtcaaca
    22561 cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca tatgaattta
    22621 aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt tttttggagg
    22681 atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt catatttccc
    22741 tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat attgctgatt
    22801 gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt tgagtgattt
    22861 cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt tgccctctcg
    22921 aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg gcgacaagca
    22981 aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca atttttcagc
    23041 agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac ctatatcttc
    23101 ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct caactaaacg
    23161 ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt gaaaagccga
    23221 aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc tttggacaca
    23281 ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag gagtcgatag
    23341 tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag
    23401 gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc gttctttatc
    23461 ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt tgacttcaaa
    23521 gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct cagtagtcgc
    23581 tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct gttttgtttt
    23641 atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt ttgcttcttc
    23701 cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc gaaatgcata
    23761 attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag gaggtccata
    23821 tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc
    23881 gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc gataagccaa
    23941 tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta tatcatattt
    24001 agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt atccaactaa
    24061 tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata gatgaatttt
    24121 tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat ttggagcatg
    24181 acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac ctaggtccag
    24241 aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg
    24301 aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg aacatcatta
    24361 ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga
    24421 ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc aacggtccgg
    24481 gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct atagcagata
    24541 gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat gctttgccta
    24601 gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg
    24661 gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg ggttggagga
    24721 aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga aaaacaagtg
    24781 acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga gatcgttgtt
    24841 cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg gatcgtttcg
    24901 caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca gaaatctgtt
    24961 tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa
    25021 tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg gcaaagcgat
    25081 ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca ctgggataat
    25141 caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac ttatttaccg
    25201 tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt agaaactgaa
    25261 agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag tggtgtcgcg
    25321 attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga gattttagat
    25381 aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt tccggaaacg
    25441 actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt acctgagttt
    25501 ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag caactgggca
    25561 acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac ggttgcgcca
    25621 agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact agatcctggt
    25681 atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc actagaaaca
    25741 gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt gctaagtatt
    25801 gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga agtagccgtt
    25861 cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca tgtatcagct
    25921 aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa cattttagca
    25981 gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac aggcacgctg
    26041 atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat gttagaacaa
    26101 gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat tttgaaaaaa
    26161 ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc aaaagactat
    26221 tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat ggaacccgag
    26281 gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat cacggagatt
    26341 actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca
    26401 agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg gatcgttcag
    26461 aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt
    26521 aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat tgctcaagag
    26581 gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga aacgaaacaa
    26641 gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga ggaatcagca
    26701 aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc agatagtcga
    26761 atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga ttattttgta
    26821 gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga aacagcacct
    26881 ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc tttcagctaa
    26941 cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc aacaatttgt
    27001 ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa
    27061 agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat tatcaaacag
    27121 atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa
    27181 agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca tgggtcccga
    27241 acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg acggacagtt
    27301 tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta tattagcaga
    27361 tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg ttgaagatac
    27421 agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta tgttagtgga
    27481 cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc tagcagaaaa
    27541 ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg tcaaattagc
    27601 tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc aaagaaggat
    27661 cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga tcagccggat
    27721 caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt attaccgtat
    27781 cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg gtactgttga
    27841 agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg gacggccaaa
    27901 acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca acgaaattta
    27961 tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg ctgtattagg
    28021 agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata ttgccatgcc
    28081 aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag gaaatccggt
    28141 agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca
    28201 ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga cgaaacaatt
    28261 aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt ctgaatttat
    28321 ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac caaaaggaga
    28381 cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta tccatacaga
    28441 tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa
    28501 attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg gcccaagtcg
    28561 cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac gtttcttcaa
    28621 agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca aatgtattac
    28681 agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag aagcactcga
    28741 tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg gagaagttag
    28801 ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa aaatcgagca
    28861 gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa
    28921 agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa ttcgtatcag
    28981 tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta aaggcagagg
    29041 gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa atgtagcaga
    29101 acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt atgatgcaga
    29161 tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc aaacagtcaa
    29221 tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata aaatggcgac
    29281 gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg tggataaaat
    29341 caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg acagatatga
    29401 gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa
    29461 aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg caaaaagatg
    29521 tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa
    29581 tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa ttcacactgt
    29641 ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga ccagaaacag
    29701 ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta
    29761 ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct gtaacgatca
    29821 ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac tatgaaacac
    29881 gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg caaaaagcgc
    29941 ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta ttttttatag
    30001 gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag atttaaaaaa
    30061 attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg acgctcccgt
    30121 cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat cagcagcaaa
    30181 agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat tagtattgaa
    30241 gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc accatcggaa
    30301 aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc gtatcttaga
    30361 taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat ttgatttaag
    30421 taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat cagcgatttc
    30481 taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg gaacgtttac
    30541 ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa aatgggcgca
    30601 aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg tggcattata
    30661 tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag tggcagctgt
    30721 tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg cattgatggg
    30781 acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact tttcgacgtt
    30841 acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa tgctacctgc
    30901 gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca gttgggttat
    30961 tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag attcctttat
    31021 gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat atgagatgat
    31081 tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat ggatcactct
    31141 tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct ctgtacctga
    31201 acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc gagcgctact
    31261 ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac agtgctgatc
    31321 ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc acgaaatgct
    31381 tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg gacattgtca
    31441 gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg attcttcatg
    31501 taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt
    31561 acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg atttcttttt
    31621 cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa
    31681 ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag ataagggaat
    31741 aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac atcgacatat
    31801 ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc gactgctcgg
    31861 atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt tggtgtgctt
    31921 tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca
    31981 tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc tttatttggt
    32041 acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt ttccttcagt
    32101 acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt atcgatcaaa
    32161 ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc ttccaaaccg
    32221 tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc tgcgtagact
    32281 tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc tagtctaaaa
    32341 caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt
    32401 gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg aaagagcgag
    32461 taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct aaagtttcat
    32521 tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac gagcgttatt
    32581 cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc
    32641 cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag ggagagaaaa
    32701 ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat
    32761 ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat aatgaaatgc
    32821 gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat acaacggata
    32881 tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg acgcttcgac
    32941 cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc ggcccagagt
    33001 atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa
    33061 aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca gaaaaccctg
    33121 caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg gggatcacgc
    33181 aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag
    33241 cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct aaacgccgcc
    33301 ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa aaatttgtgg
    33361 cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat tttgataccg
    33421 ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat atggttcgcg
    33481 gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca aaaatggggg
    33541 cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa ctaggtggtc
    33601 cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg actatggagg
    33661 cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc gcactcggag
    33721 acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag
    33781 ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca gataaagcga
    33841 atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg attaatgtca
    33901 aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat gaacattttg
    33961 atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa tggcacaaag
    34021 aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta cattaaaagg
    34081 atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac gtgatcgtga
    34141 aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga
    34201 taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac gcggagaaaa
    34261 tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg aaatcgtgat
    34321 tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa tcagtgacga
    34381 aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta aaaacatcaa
    34441 attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg actttctcga
    34501 tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact acctagttcc
    34561 ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat tgtttaaaca
    34621 attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga
    34681 agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa caacgttctt
    34741 aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa tgaaagaagt
    34801 tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag caatggctcg
    34861 ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc taagcgatac
    34921 agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa
    34981 agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg atcagttagg
    35041 tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg
    35101 attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca ttaaagcaac
    35161 agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc
    35221 tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg atgaatcgaa
    35281 attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag aagaaggtcg
    35341 atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc cgttattggc
    35401 tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg gttatgaatt
    35461 aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac tagaaactct
    35521 aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag ctttagatta
    35581 cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa tgttattagc
    35641 cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag cagcagatgt
    35701 gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga atattgatgt
    35761 aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt
    35821 tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata taaagcgggg
    35881 cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga ccaagctttc
    35941 ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt atcagccagg
    36001 acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt tgtcaaaatc
    36061 actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata tacctttgtt
    36121 tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta tcacagtcac
    36181 tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg atccaatcat
    36241 ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt tgagaaacgg
    36301 cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc
    36361 gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg gaattctatt
    36421 tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa aagtaactga
    36481 tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac atccagaaaa
    36541 agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca atgaagcaga
    36601 aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac gatacgaact
    36661 gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga
    36721 aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt ttgacaaaaa
    36781 ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc taccatagta
    36841 gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga aaaaaagcat
    36901 gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg acacgattct
    36961 acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat gaaaagttgg
    37021 aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact gggtttgatg
    37081 ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca actagtatga
    37141 aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg atcacggatc
    37201 tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt tatgtcagaa
    37261 gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg gcttatatca
    37321 ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt
    37381 atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga tttgctaatg
    37441 agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat ttggtttggc
    37501 cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc ttttatactg
    37561 tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca ccaggaggcg
    37621 atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg
    37681 cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa
    37741 atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc ttcttctttt
    37801 tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt ctgttatgcg
    37861 tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc
    37921 cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag tttccagtta
    37981 tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg catactttag
    38041 tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg gagcgtccgc
    38101 tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc agcaaaccga
    38161 tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca attgcactcg
    38221 ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa
    38281 aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa aacaccacta
    38341 gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa gggaataagg
    38401 cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct gcaacggatc
    38461 tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa ttatatgcca
    38521 gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact gttctgattg
    38581 gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg
    38641 gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg
    38701 tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg attgattggt
    38761 acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat ccaaatcctg
    38821 aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga agttcaagta
    38881 cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca ggcaaaaaca
    38941 tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat atagattcag
    39001 gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt
    39061 tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct gcatggatca
    39121 tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca ttgttatgtt
    39181 cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga tctttacaga
    39241 ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc tgcagtgtat
    39301 tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc taatttgcct
    39361 tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg gtaaacaaat
    39421 tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc taagaaccga
    39481 tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact tgaaatatcg
    39541 atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg tttattaggg
    39601 ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac taagtcacaa
    39661 gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac agtcatttgt
    39721 tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg aggttgaagt
    39781 tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt
    39841 cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg agcaagcagc
    39901 acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat atcttctgcg
    39961 tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat
    40021 agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc aaacctctat
    40081 tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt atcgatttat
    40141 gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct atgagaataa
    40201 agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta cttggtggga
    40261 cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg caaaagaaga
    40321 actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt ttccagttaa
    40381 aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga aatcgacaaa
    40441 tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta aaggcggaat
    40501 ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt
    40561 gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg aaccaatcga
    40621 cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac gacgaacaca
    40681 agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa
    40741 aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc
    40801 agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt gcgttccaat
    40861 atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa ttagaaggac
    40921 attttttgag gtgattgaat gcgtttttta catacagccg attggcatat cggaaaaaaa
    40981 ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat actagatatc
    41041 gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga taggagtgtc
    41101 ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa tttaaaggaa
    41161 aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact ttcagcgggt
    41221 acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga agcatttgaa
    41281 ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc aattgcagcg
    41341 agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc caaggtgata
    41401 gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag ccattttttt
    41461 gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg gttggattcg
    41521 atccctggtg aactacttac accgtttgat tatgtagcac taggacattt gcataatcaa
    41581 gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt ctctttatct
    41641 gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct ggagttgaat
    41701 tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt caaagaattg
    41761 ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat ctatctcact
    41821 gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc tcgtattttg
    41881 gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa
    41941 atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga agagcagcta
    42001 actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga aatggaatga
    42061 tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat caatgagacc
    42121 gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa gacaggagct
    42181 ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga
    42241 cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga aacaagcgtg
    42301 actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt
    42361 ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt
    42421 tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc agatcagttg
    42481 atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt actaccccaa
    42541 ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt tttacgtcat
    42601 ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa
    42661 cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt
    42721 ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca atggacaaac
    42781 catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac ggaacttcag
    42841 gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa aaaaagttat
    42901 gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa agacgaaata
    42961 gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt cgaaccaatc
    43021 cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa
    43081 acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac
    43141 cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg
    43201 atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca attaacaatt
    43261 tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc tatctatcag
    43321 gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca agaagatcgt
    43381 cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga aaaacaaaaa
    43441 gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc aactgaagaa
    43501 ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca gctgaaaagc
    43561 caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg ggagccatgt
    43621 cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca gccaaataag
    43681 gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt agaaagaact
    43741 cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca attatcagat
    43801 ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga acacttgcaa
    43861 caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac aactcttgta
    43921 gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag aatagagctg
    43981 gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta caaacagcag
    44041 cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa
    44101 caattgcaag atacggatag taactgttta gaagaggaga tacgacgact tgaaaaacag
    44161 ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca attgttgcaa
    44221 gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga aaaaatagat
    44281 gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca gtcgtatttt
    44341 tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa agaaaaccaa
    44401 cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta
    44461 gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca acagttagaa
    44521 gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca agaacaaaaa
    44581 attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag ccagacgaaa
    44641 ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga aaatcctaag
    44701 aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt acaagtagca
    44761 aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc ggataccatt
    44821 ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca ggcaggtatg
    44881 tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact ttctttagca
    44941 ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga tgcattgttt
    45001 attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat ggaagcttta
    45061 gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag agaattgaaa
    45121 gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag caaaatacgc
    45181 tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc agaaaaagtc
    45241 attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa
    45301 gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt tgatttggat
    45361 ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca taaatattgt
    45421 aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg tatcatgaaa
    45481 aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat cattgcaaaa
    45541 ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt gattattgaa
    45601 taccaagtag aaacgattga gactaatcag tatcacccag agctttctat tttggcactt
    45661 tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat
    45721 gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca gtaccagttt
    45781 cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga
    45841 agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg aaaactagat
    45901 aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca gatgaatcag
    45961 actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca attttctgat
    46021 caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt gctgcaagca
    46081 ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca agggacattt
    46141 tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt gattcgccga
    46201 ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt taaagagatt
    46261 atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga agaggaagta
    46321 gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa aatccaagct
    46381 cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca tgaggtctcg
    46441 acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt acttgatcta
    46501 ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt gccaagcggg
    46561 gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc
    46621 agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca accgaaaatc
    46681 gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg gatagaggag
    46741 caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta tacattagaa
    46801 aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca agtgttgcaa
    46861 caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa aaatcaagga
    46921 ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg
    46981 atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa aggattgaat
    47041 gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt aggtcattat
    47101 cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca gacaattgcc
    47161 tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt agcgcctgcg
    47221 agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt aagtatccaa
    47281 gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac agatatccga
    47341 gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca aaaaattgat
    47401 tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac tgcacaagca
    47461 ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat cgagaataat
    47521 ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc gactaaaaca
    47581 aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg
    47641 cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa tctatattct
    47701 gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa
    47761 atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact tgctggtctg
    47821 acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt
    47881 tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa caaacgaaga
    47941 gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga gttagccgag
    48001 ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg gttaagtatg
    48061 gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa ggctggggga
    48121 actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg gtggaatcct
    48181 gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa
    48241 gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact ccagcaagaa
    48301 aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac gaaactgact
    48361 gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa
    48421 agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt tgatttaaaa
    48481 aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat ttttttattt
    48541 ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga tcaaaagaag
    48601 aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa
    48661 gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt
    48721 ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa atctcttttt
    48781 ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga atccaattgt
    48841 tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt gtgataattc
    48901 ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc
    48961 agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct tcttccgtaa
    49021 tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc atatattcta
    49081 gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg cggaaccgga
    49141 tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg accgcgtttc
    49201 gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact gatttaatca
    49261 aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac tattgtaaca
    49321 gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt tctatttcct
    49381 tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa acacgtcaat
    49441 aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt taatgcaatc
    49501 tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac tattcaaaca
    49561 taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagt
//
LOCUS       DAZUMB010000004        48797 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000004 DAZUMB010000000
VERSION     DAZUMB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.004"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..1263
                     /locus_tag="ACG80L_000159"
                     /pseudo
     CDS             136..1263
                     /locus_tag="ACG80L_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            1361..1549
                     /locus_tag="ACG80L_000160"
     CDS             1361..1549
                     /locus_tag="ACG80L_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS5022453.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1607..1960)
                     /locus_tag="ACG80L_000161"
     CDS             complement(1607..1960)
                     /locus_tag="ACG80L_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5022454.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2491..2913
                     /locus_tag="ACG80L_000162"
     CDS             2491..2913
                     /locus_tag="ACG80L_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5022455.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2910..3140
                     /locus_tag="ACG80L_000163"
     CDS             2910..3140
                     /locus_tag="ACG80L_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5022456.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3638..3838
                     /locus_tag="ACG80L_000164"
     CDS             3638..3838
                     /locus_tag="ACG80L_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS5022457.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3865..5058
                     /locus_tag="ACG80L_000165"
     CDS             3865..5058
                     /locus_tag="ACG80L_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5022458.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5126..6691)
                     /gene="guaA"
                     /locus_tag="ACG80L_000166"
     CDS             complement(5126..6691)
                     /gene="guaA"
                     /locus_tag="ACG80L_000166"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5022459.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6750..7673)
                     /gene="coaA"
                     /locus_tag="ACG80L_000167"
     CDS             complement(6750..7673)
                     /gene="coaA"
                     /locus_tag="ACG80L_000167"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS5022460.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7869..8474
                     /locus_tag="ACG80L_000168"
     CDS             7869..8474
                     /locus_tag="ACG80L_000168"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5022461.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8782..9798
                     /locus_tag="ACG80L_000169"
     CDS             8782..9798
                     /locus_tag="ACG80L_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5022462.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9939..11240
                     /locus_tag="ACG80L_000170"
     CDS             9939..11240
                     /locus_tag="ACG80L_000170"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS5022463.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11286..11948
                     /gene="deoC"
                     /locus_tag="ACG80L_000171"
     CDS             11286..11948
                     /gene="deoC"
                     /locus_tag="ACG80L_000171"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS5022464.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11969..12367
                     /locus_tag="ACG80L_000172"
     CDS             11969..12367
                     /locus_tag="ACG80L_000172"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS5022465.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12433..13515
                     /locus_tag="ACG80L_000173"
     CDS             12433..13515
                     /locus_tag="ACG80L_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5022466.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13727..15289
                     /locus_tag="ACG80L_000174"
     CDS             13727..15289
                     /locus_tag="ACG80L_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022467.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15282..16415
                     /locus_tag="ACG80L_000175"
     CDS             15282..16415
                     /locus_tag="ACG80L_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022468.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16415..17371
                     /locus_tag="ACG80L_000176"
     CDS             16415..17371
                     /locus_tag="ACG80L_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022469.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17470..18636
                     /gene="deoB"
                     /locus_tag="ACG80L_000177"
     CDS             17470..18636
                     /gene="deoB"
                     /locus_tag="ACG80L_000177"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS5022470.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18660..19478
                     /locus_tag="ACG80L_000178"
     CDS             18660..19478
                     /locus_tag="ACG80L_000178"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5022471.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19495..20202
                     /gene="deoD"
                     /locus_tag="ACG80L_000179"
     CDS             19495..20202
                     /gene="deoD"
                     /locus_tag="ACG80L_000179"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5022472.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(20500..21186)
                     /gene="gpmA"
                     /locus_tag="ACG80L_000180"
     CDS             complement(20500..21186)
                     /gene="gpmA"
                     /locus_tag="ACG80L_000180"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5022473.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(21358..21939)
                     /locus_tag="ACG80L_000181"
     CDS             complement(21358..21939)
                     /locus_tag="ACG80L_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5022474.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(22072..22755)
                     /gene="rpiA"
                     /locus_tag="ACG80L_000182"
     CDS             complement(22072..22755)
                     /gene="rpiA"
                     /locus_tag="ACG80L_000182"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS5022475.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            23074..23487
                     /gene="rpsL"
                     /locus_tag="ACG80L_000183"
     CDS             23074..23487
                     /gene="rpsL"
                     /locus_tag="ACG80L_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS5022476.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            23571..24041
                     /gene="rpsG"
                     /locus_tag="ACG80L_000184"
     CDS             23571..24041
                     /gene="rpsG"
                     /locus_tag="ACG80L_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS5022477.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            24135..26222
                     /gene="fusA"
                     /locus_tag="ACG80L_000185"
     CDS             24135..26222
                     /gene="fusA"
                     /locus_tag="ACG80L_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5022478.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            26394..27581
                     /gene="tuf"
                     /locus_tag="ACG80L_000186"
     CDS             26394..27581
                     /gene="tuf"
                     /locus_tag="ACG80L_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5022479.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27988..28296
                     /gene="rpsJ"
                     /locus_tag="ACG80L_000187"
     CDS             27988..28296
                     /gene="rpsJ"
                     /locus_tag="ACG80L_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS5022480.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            28327..28956
                     /gene="rplC"
                     /locus_tag="ACG80L_000188"
     CDS             28327..28956
                     /gene="rplC"
                     /locus_tag="ACG80L_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS5022481.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28984..29607
                     /gene="rplD"
                     /locus_tag="ACG80L_000189"
     CDS             28984..29607
                     /gene="rplD"
                     /locus_tag="ACG80L_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS5022482.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            29607..29897
                     /gene="rplW"
                     /locus_tag="ACG80L_000190"
     CDS             29607..29897
                     /gene="rplW"
                     /locus_tag="ACG80L_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS5022483.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29936..30769
                     /gene="rplB"
                     /locus_tag="ACG80L_000191"
     CDS             29936..30769
                     /gene="rplB"
                     /locus_tag="ACG80L_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS5022484.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30812..31090
                     /gene="rpsS"
                     /locus_tag="ACG80L_000192"
     CDS             30812..31090
                     /gene="rpsS"
                     /locus_tag="ACG80L_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS5022485.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            31113..31460
                     /gene="rplV"
                     /locus_tag="ACG80L_000193"
     CDS             31113..31460
                     /gene="rplV"
                     /locus_tag="ACG80L_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS5022486.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            31474..32130
                     /gene="rpsC"
                     /locus_tag="ACG80L_000194"
     CDS             31474..32130
                     /gene="rpsC"
                     /locus_tag="ACG80L_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS5022487.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            32133..32567
                     /gene="rplP"
                     /locus_tag="ACG80L_000195"
     CDS             32133..32567
                     /gene="rplP"
                     /locus_tag="ACG80L_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS5022488.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            32557..32745
                     /gene="rpmC"
                     /locus_tag="ACG80L_000196"
     CDS             32557..32745
                     /gene="rpmC"
                     /locus_tag="ACG80L_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS5022489.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            32770..33036
                     /gene="rpsQ"
                     /locus_tag="ACG80L_000197"
     CDS             32770..33036
                     /gene="rpsQ"
                     /locus_tag="ACG80L_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS5022490.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            33094..33462
                     /gene="rplN"
                     /locus_tag="ACG80L_000198"
     CDS             33094..33462
                     /gene="rplN"
                     /locus_tag="ACG80L_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS5022491.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            33499..33807
                     /gene="rplX"
                     /locus_tag="ACG80L_000199"
     CDS             33499..33807
                     /gene="rplX"
                     /locus_tag="ACG80L_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS5022492.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33835..34374
                     /gene="rplE"
                     /locus_tag="ACG80L_000200"
     CDS             33835..34374
                     /gene="rplE"
                     /locus_tag="ACG80L_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS5022493.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            34393..34578
                     /locus_tag="ACG80L_000201"
     CDS             34393..34578
                     /locus_tag="ACG80L_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS5022494.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            34614..35012
                     /gene="rpsH"
                     /locus_tag="ACG80L_000202"
     CDS             34614..35012
                     /gene="rpsH"
                     /locus_tag="ACG80L_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS5022495.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            35044..35580
                     /gene="rplF"
                     /locus_tag="ACG80L_000203"
     CDS             35044..35580
                     /gene="rplF"
                     /locus_tag="ACG80L_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS5022496.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            35741..36097
                     /gene="rplR"
                     /locus_tag="ACG80L_000204"
     CDS             35741..36097
                     /gene="rplR"
                     /locus_tag="ACG80L_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS5022497.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            36118..36618
                     /gene="rpsE"
                     /locus_tag="ACG80L_000205"
     CDS             36118..36618
                     /gene="rpsE"
                     /locus_tag="ACG80L_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS5022498.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            36633..36812
                     /gene="rpmD"
                     /locus_tag="ACG80L_000206"
     CDS             36633..36812
                     /gene="rpmD"
                     /locus_tag="ACG80L_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS5022499.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36856..37296
                     /gene="rplO"
                     /locus_tag="ACG80L_000207"
     CDS             36856..37296
                     /gene="rplO"
                     /locus_tag="ACG80L_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS5022500.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            37296..38591
                     /gene="secY"
                     /locus_tag="ACG80L_000208"
     CDS             37296..38591
                     /gene="secY"
                     /locus_tag="ACG80L_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS5022501.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            38653..39300
                     /locus_tag="ACG80L_000209"
     CDS             38653..39300
                     /locus_tag="ACG80L_000209"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS5022502.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            39491..39709
                     /gene="infA"
                     /locus_tag="ACG80L_000210"
     CDS             39491..39709
                     /gene="infA"
                     /locus_tag="ACG80L_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS5022503.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            39742..39858
                     /gene="rpmJ"
                     /locus_tag="ACG80L_000211"
     CDS             39742..39858
                     /gene="rpmJ"
                     /locus_tag="ACG80L_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS5022504.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39876..40241
                     /gene="rpsM"
                     /locus_tag="ACG80L_000212"
     CDS             39876..40241
                     /gene="rpsM"
                     /locus_tag="ACG80L_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS5022505.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            40269..40658
                     /gene="rpsK"
                     /locus_tag="ACG80L_000213"
     CDS             40269..40658
                     /gene="rpsK"
                     /locus_tag="ACG80L_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS5022506.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40738..41676
                     /locus_tag="ACG80L_000214"
     CDS             40738..41676
                     /locus_tag="ACG80L_000214"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS5022507.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            41704..42084
                     /gene="rplQ"
                     /locus_tag="ACG80L_000215"
     CDS             41704..42084
                     /gene="rplQ"
                     /locus_tag="ACG80L_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS5022508.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            42356..43195
                     /locus_tag="ACG80L_000216"
     CDS             42356..43195
                     /locus_tag="ACG80L_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5022509.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            43171..44040
                     /locus_tag="ACG80L_000217"
     CDS             43171..44040
                     /locus_tag="ACG80L_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5022510.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            44030..44827
                     /locus_tag="ACG80L_000218"
     CDS             44030..44827
                     /locus_tag="ACG80L_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5022511.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            45030..45776
                     /gene="truA"
                     /locus_tag="ACG80L_000219"
     CDS             45030..45776
                     /gene="truA"
                     /locus_tag="ACG80L_000219"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5022512.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45837..46043)
                     /locus_tag="ACG80L_000220"
     CDS             complement(45837..46043)
                     /locus_tag="ACG80L_000220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022513.1"
                     /translation="MVTEKIGNKKMIHLSTPRPQVTIFIKDEKLFFEIALCDSELNDR
                     LTTSFLNGVPEVASSKISRNFAKF"
     gene            complement(46109..48778)
                     /locus_tag="ACG80L_000221"
     CDS             complement(46109..48778)
                     /locus_tag="ACG80L_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS5022514.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        15751 a         8935 c        10409 g        13702 t
ORIGIN      
        1 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
       61 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
      121 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
      181 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
      241 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
      301 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
      361 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
      421 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
      481 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
      541 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
      601 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
      661 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
      721 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
      781 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
      841 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
      901 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
      961 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
     1021 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
     1081 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
     1141 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
     1201 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
     1261 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
     1321 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
     1381 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
     1441 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
     1501 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
     1561 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
     1621 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
     1681 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
     1741 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
     1801 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
     1861 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
     1921 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
     1981 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
     2041 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
     2101 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
     2161 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
     2221 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
     2281 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
     2341 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
     2401 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
     2461 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
     2521 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
     2581 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
     2641 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
     2701 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
     2761 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
     2821 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
     2881 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
     2941 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
     3001 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
     3061 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
     3121 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
     3181 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
     3241 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
     3301 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
     3361 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
     3421 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
     3481 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
     3541 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
     3601 ggagttttcc tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga
     3661 tttagttgag ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga
     3721 attgatgatt acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg
     3781 tgaagctgtt gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt
     3841 tttgcacagt aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga
     3901 acctattatt ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat
     3961 cgtagtggat tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg
     4021 aaaccagaag aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa
     4081 attttccttc catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca
     4141 actattaaga agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt
     4201 gatgtgcaaa attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct
     4261 gttcaaggac tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca
     4321 aatccatcta agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca
     4381 aaagaagaat ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt
     4441 ttatttatct ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc
     4501 attcaatggg aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat
     4561 tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat
     4621 attgtattag ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat
     4681 ctaattcaga cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta
     4741 tcatatgcta ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg
     4801 ttacggcatt ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa
     4861 gatcgtctag gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct
     4921 aacagtaatt tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa
     4981 caaaattatg atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa
     5041 atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat
     5101 aagaggattt ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag
     5161 tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt
     5221 tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta
     5281 cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc
     5341 ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga
     5401 tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt
     5461 cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat
     5521 caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg
     5581 gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga
     5641 tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta
     5701 ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga
     5761 attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta
     5821 agaagcgatc tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt
     5881 ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca
     5941 attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg
     6001 aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat
     6061 cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc
     6121 gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa
     6181 aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt
     6241 caaagccttc aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt
     6301 ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat
     6361 attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac
     6421 catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg
     6481 cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg
     6541 cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac
     6601 gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt
     6661 caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta
     6721 tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg
     6781 atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat
     6841 tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct
     6901 tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc
     6961 agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca
     7021 tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc
     7081 gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga
     7141 atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt
     7201 acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg
     7261 tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc
     7321 agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt
     7381 gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg
     7441 tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga
     7501 taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca
     7561 ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa
     7621 aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc
     7681 tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact
     7741 taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt
     7801 cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga
     7861 tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac
     7921 aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt
     7981 cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt
     8041 tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt
     8101 tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc
     8161 tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata
     8221 tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg
     8281 ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg
     8341 gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg
     8401 aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa
     8461 gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc
     8521 atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt
     8581 ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag
     8641 atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa
     8701 catactttcc ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt
     8761 agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc
     8821 gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag
     8881 acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct
     8941 tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat
     9001 ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg
     9061 atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt
     9121 tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat
     9181 gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat
     9241 cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg
     9301 ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat
     9361 caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt
     9421 aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa
     9481 aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc
     9541 ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata
     9601 ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag
     9661 ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct
     9721 caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa
     9781 gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt
     9841 tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat
     9901 aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg
     9961 aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact
    10021 atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc
    10081 aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg
    10141 tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg
    10201 gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta
    10261 aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc
    10321 ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag
    10381 tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc
    10441 gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa
    10501 aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca
    10561 tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg
    10621 ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg
    10681 gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc
    10741 tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga
    10801 caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa
    10861 aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac
    10921 gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa
    10981 aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca
    11041 aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg
    11101 tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca
    11161 ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc
    11221 atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa
    11281 aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag
    11341 aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca
    11401 atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta
    11461 cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag
    11521 atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat
    11581 caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc
    11641 gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag
    11701 cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa
    11761 caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg
    11821 gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg
    11881 gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag
    11941 gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac
    12001 agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg
    12061 tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg
    12121 attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac
    12181 atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg
    12241 tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa
    12301 tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga
    12361 tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga
    12421 ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca
    12481 gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc
    12541 tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca
    12601 ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga
    12661 ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt
    12721 gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca
    12781 gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc
    12841 atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt
    12901 gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta
    12961 aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg
    13021 aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca
    13081 gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat
    13141 gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc
    13201 gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca
    13261 ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta
    13321 gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt
    13381 tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa
    13441 aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact
    13501 cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg
    13561 gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg
    13621 gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt
    13681 agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa
    13741 tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa
    13801 catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg
    13861 caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt
    13921 gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg
    13981 gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt
    14041 gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca
    14101 aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc
    14161 agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact
    14221 gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat
    14281 catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga
    14341 aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt
    14401 caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc
    14461 attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct
    14521 agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc
    14581 tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc
    14641 tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac
    14701 taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca
    14761 taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca
    14821 tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag
    14881 agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt
    14941 atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct
    15001 gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa
    15061 acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga
    15121 agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat
    15181 cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt
    15241 agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa
    15301 aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga
    15361 tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag
    15421 cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta
    15481 cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac
    15541 aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa
    15601 aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg
    15661 ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga
    15721 tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct
    15781 atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat
    15841 ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg
    15901 cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg
    15961 ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt
    16021 tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa
    16081 cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg
    16141 tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta
    16201 ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca
    16261 atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca
    16321 tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac
    16381 caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt
    16441 gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac
    16501 tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc
    16561 ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg
    16621 gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc
    16681 aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc
    16741 tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac
    16801 gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat
    16861 tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg
    16921 ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca
    16981 agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc
    17041 tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc
    17101 agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg
    17161 gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat
    17221 cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc
    17281 atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc
    17341 aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac
    17401 gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa
    17461 ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa
    17521 gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa
    17581 gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt
    17641 gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct
    17701 gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca
    17761 tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct
    17821 ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt
    17881 gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag
    17941 atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt
    18001 cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg
    18061 ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc
    18121 ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag
    18181 atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg
    18241 gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc
    18301 aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat
    18361 gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac
    18421 ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg
    18481 cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct
    18541 caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact
    18601 gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa
    18661 tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa
    18721 aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg
    18781 aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg
    18841 ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag
    18901 ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga
    18961 tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat
    19021 catttgtacc aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc
    19081 ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt
    19141 atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta
    19201 aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa
    19261 tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag
    19321 cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta
    19381 tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt
    19441 ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt
    19501 gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct
    19561 ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta
    19621 cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca
    19681 ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta
    19741 aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat
    19801 ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc
    19861 tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa
    19921 gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat
    19981 gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca
    20041 gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt
    20101 agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat
    20161 gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct
    20221 gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg
    20281 ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct
    20341 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    20401 tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact
    20461 gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc
    20521 aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc
    20581 catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc
    20641 atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca
    20701 gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt
    20761 gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa
    20821 tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc
    20881 agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca
    20941 tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt
    21001 gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat
    21061 tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca
    21121 accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt
    21181 tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt
    21241 tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa
    21301 acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta
    21361 gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac
    21421 tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg
    21481 tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt
    21541 acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc
    21601 taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc
    21661 aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg
    21721 cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc
    21781 agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc
    21841 atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac
    21901 gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg
    21961 attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt
    22021 ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg
    22081 acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat
    22141 gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct
    22201 ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt
    22261 taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat
    22321 atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag
    22381 tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac
    22441 tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat
    22501 ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg
    22561 tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg
    22621 taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc
    22681 ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa
    22741 ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac
    22801 tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt
    22861 caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg
    22921 ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa
    22981 ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac
    23041 tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt
    23101 aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac
    23161 agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt
    23221 gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt
    23281 ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa
    23341 cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat
    23401 atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa
    23461 tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta
    23521 ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta
    23581 aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa
    23641 ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct
    23701 ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac
    23761 aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa
    23821 actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg
    23881 tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa
    23941 tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa
    24001 tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg
    24061 gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag
    24121 gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca
    24181 tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta
    24241 aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag
    24301 aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc
    24361 gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat
    24421 tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa
    24481 ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca
    24541 aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc
    24601 aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta
    24661 tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag
    24721 aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg
    24781 aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca
    24841 ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag
    24901 tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg
    24961 attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg
    25021 aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta
    25081 tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat
    25141 caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac
    25201 agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg
    25261 ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta
    25321 ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa
    25381 aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt
    25441 tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc
    25501 acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg
    25561 cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg
    25621 tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg
    25681 aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat
    25741 acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct
    25801 atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa
    25861 atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta
    25921 atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag
    25981 gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg
    26041 ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta
    26101 caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg
    26161 aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat
    26221 aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat atttttaata
    26281 tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat
    26341 atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa
    26401 aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc
    26461 atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc
    26521 aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa
    26581 tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc
    26641 caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta
    26701 tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt
    26761 ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg
    26821 acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat
    26881 tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg
    26941 cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc
    27001 cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg
    27061 gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag
    27121 ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt
    27181 tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg
    27241 ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc
    27301 atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc
    27361 cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg
    27421 ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg
    27481 aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac
    27541 gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt
    27601 aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga
    27661 gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca
    27721 cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca
    27781 agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta
    27841 gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca
    27901 cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt
    27961 ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa
    28021 gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga
    28081 actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc
    28141 atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa
    28201 cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac
    28261 ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt
    28321 gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa
    28381 tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg
    28441 aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa
    28501 gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc
    28561 ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca
    28621 gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga
    28681 ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt
    28741 taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa
    28801 cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt
    28861 gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct
    28921 ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa
    28981 ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt
    29041 aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg
    29101 caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc
    29161 ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc
    29221 tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac
    29281 aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct
    29341 gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc
    29401 aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt
    29461 aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat
    29521 aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt
    29581 actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga
    29641 tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc
    29701 gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa
    29761 acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata
    29821 caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat
    29881 tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc
    29941 gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc
    30001 tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg
    30061 acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata
    30121 tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga
    30181 atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc
    30241 ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc
    30301 agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca
    30361 caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc
    30421 tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg
    30481 tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt
    30541 aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg
    30601 tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg
    30661 acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa
    30721 aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc
    30781 atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt
    30841 tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt
    30901 tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc
    30961 agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa
    31021 attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac
    31081 gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag
    31141 caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca
    31201 gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg
    31261 gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag
    31321 atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt
    31381 tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg
    31441 tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga
    31501 atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct
    31561 gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct
    31621 gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca
    31681 gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa
    31741 ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat
    31801 ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc
    31861 cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa
    31921 actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa
    31981 ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat
    32041 acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact
    32101 aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc
    32161 gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg
    32221 gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg
    32281 ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc
    32341 ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac
    32401 ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc
    32461 ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta
    32521 agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa
    32581 ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt
    32641 agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa
    32701 tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc
    32761 attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca
    32821 gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat
    32881 ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa
    32941 gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt
    33001 ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga
    33061 ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa
    33121 gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt
    33181 cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt
    33241 ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct
    33301 cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat
    33361 gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac
    33421 aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa
    33481 aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga
    33541 aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt
    33601 gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt
    33661 ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact
    33721 ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa
    33781 aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac
    33841 cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat
    33901 agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat
    33961 gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt
    34021 caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga
    34081 atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa
    34141 ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt
    34201 gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat
    34261 tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct
    34321 gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag
    34381 gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt
    34441 tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag
    34501 ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg
    34561 aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca
    34621 tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg
    34681 agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg
    34741 aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt
    34801 tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac
    34861 caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta
    34921 tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata
    34981 tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt
    35041 taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa
    35101 gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca
    35161 gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa
    35221 gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc
    35281 agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa
    35341 ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct
    35401 ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa
    35461 gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt
    35521 aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa
    35581 tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac
    35641 cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg
    35701 cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa
    35761 gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga
    35821 gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga
    35881 cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac
    35941 aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg
    36001 cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt
    36061 agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg
    36121 gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta
    36181 acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac
    36241 aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt
    36301 aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc
    36361 ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa
    36421 ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca
    36481 gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt
    36541 gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt
    36601 gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac
    36661 gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa
    36721 agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg
    36781 tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa
    36841 ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta
    36901 cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa
    36961 aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca
    37021 ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca
    37081 gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct
    37141 ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg
    37201 gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca
    37261 attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt
    37321 ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc
    37381 gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt
    37441 ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca
    37501 ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa
    37561 tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac
    37621 tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca
    37681 ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt
    37741 acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac
    37801 agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt
    37861 ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt
    37921 cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg
    37981 tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac
    38041 gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta
    38101 ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga
    38161 gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata
    38221 cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct
    38281 ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac
    38341 caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa
    38401 gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg
    38461 tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg
    38521 tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat
    38581 tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta
    38641 ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa
    38701 gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc
    38761 gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt
    38821 gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat
    38881 acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg
    38941 gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt
    39001 gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact
    39061 taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat
    39121 gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac
    39181 atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt
    39241 gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag
    39301 cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat
    39361 gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt
    39421 gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga
    39481 ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt
    39541 gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc
    39601 aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt
    39661 gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt
    39721 aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat
    39781 tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat
    39841 aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata
    39901 ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta
    39961 ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa
    40021 cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc
    40081 ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa
    40141 tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc
    40201 gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt
    40261 aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag
    40321 caggtattgc acatatccat tctacattca ataacactat catcatgatc actgatactc
    40381 acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat
    40441 caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg
    40501 gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc
    40561 gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc
    40621 ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt
    40681 ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg
    40741 attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc
    40801 atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt
    40861 ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat
    40921 gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa
    40981 ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga
    41041 cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa
    41101 gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa
    41161 cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt
    41221 cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca
    41281 cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca
    41341 attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt
    41401 tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca
    41461 caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac
    41521 aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct
    41581 gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg
    41641 cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc
    41701 aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat
    41761 ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa
    41821 gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt
    41881 cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac
    41941 atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa
    42001 cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca
    42061 atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt
    42121 ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag
    42181 cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc
    42241 tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa
    42301 ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga
    42361 gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc
    42421 attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa
    42481 tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg
    42541 gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat
    42601 ggttggaatg atttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga
    42661 cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa
    42721 agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc
    42781 aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat
    42841 tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac
    42901 gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga
    42961 tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg
    43021 tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc
    43081 atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata
    43141 tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc
    43201 acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc
    43261 aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt
    43321 cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat
    43381 attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc
    43441 tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt
    43501 cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa
    43561 caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa
    43621 atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat
    43681 gaaccaacgg caggacttga tccccagggc agaaaagaaa tgatggagat gttttggcgt
    43741 cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc
    43801 aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg
    43861 caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc
    43921 accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca
    43981 gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa
    44041 attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc
    44101 taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac
    44161 gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa
    44221 attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca
    44281 ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga
    44341 atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc
    44401 gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct
    44461 tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc
    44521 tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc
    44581 tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt
    44641 gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga
    44701 agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa
    44761 tagagacacg atggttatca ttgcttttgt attattaact gtgctactga tctttttaag
    44821 aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag
    44881 gatgcaagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct
    44941 ctttctttta tctgaaaaga aagaacgaaa aaattttata gaaaaatcat tattccagat
    45001 aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcgtacg
    45061 acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag
    45121 tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa
    45181 ggacagatgc cggagtacat gcgatcgggc aagtaatcca ttttgattat ccagaggagc
    45241 gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa
    45301 gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt
    45361 atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt
    45421 attttcctta cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga
    45481 ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa atacgtacga
    45541 tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg
    45601 gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca
    45661 atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag
    45721 caggacctac agcacaccct gaaggacttt atttgtatga agtgaaatat gaataagagg
    45781 ttatgataga agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa
    45841 aattttgcaa aatttcggct tattttcgaa gaagctactt ctggaacacc gtttaagaaa
    45901 gaggtcgtga gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc
    45961 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    46021 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg
    46081 gacagtctga acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc
    46141 gtggttgttc ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt
    46201 accagacata aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa
    46261 atccttcagg caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca
    46321 agaatgcgtc tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc
    46381 ctttgtcata aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag
    46441 aagcttttct gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg
    46501 cttgataggc tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg
    46561 tcccataaaa acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat
    46621 agtattgacc agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg
    46681 cactagaact ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat
    46741 ttgcaggcac atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt
    46801 aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg
    46861 tatagaatgt aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac
    46921 tggtcaaatt atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg
    46981 ctagtggcaa tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc
    47041 ggaagccata tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa
    47101 acccatccat cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac
    47161 ggaaacctaa tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac
    47221 tgataccact gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca
    47281 agcggtaaaa cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat
    47341 aaggatcagt atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca
    47401 tactgttcgt attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat
    47461 gattgaatga aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga
    47521 aaaccactgt caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga
    47581 tgccccatgc caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac
    47641 catcgtcttg ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga
    47701 acagataggc ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat
    47761 tcaatggaac aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt
    47821 tatttttcaa cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca
    47881 gacctgcgta aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat
    47941 ttttcatgat tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca
    48001 gtgcctcgcc atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc
    48061 cgcctgccag taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt
    48121 ttgtcaatag acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat
    48181 aaaaactgga taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat
    48241 ccataacacg atgaatcccc aaaatcacta atggaagata aacaaatgca tccagccaca
    48301 tgatgatctc cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa
    48361 catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac
    48421 agccgacctt aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa
    48481 agaacaccag tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca
    48541 atcctagaga agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga
    48601 agctggcatg aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc
    48661 ttccaggata gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga
    48721 aactagctgc tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat
    48781 ttattttcca ccaaatt
//
LOCUS       DAZUMB010000005        46791 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000005 DAZUMB010000000
VERSION     DAZUMB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.005"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1129
                     /locus_tag="ACG80L_000222"
     CDS             128..1129
                     /locus_tag="ACG80L_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS5022515.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1148..2167
                     /locus_tag="ACG80L_000223"
     CDS             1148..2167
                     /locus_tag="ACG80L_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS5022516.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2269..2985)
                     /locus_tag="ACG80L_000224"
     CDS             complement(2269..2985)
                     /locus_tag="ACG80L_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5022517.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(2954..3739)
                     /locus_tag="ACG80L_000225"
     CDS             complement(2954..3739)
                     /locus_tag="ACG80L_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5022518.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3730..4479)
                     /locus_tag="ACG80L_000226"
     CDS             complement(3730..4479)
                     /locus_tag="ACG80L_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5022519.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4777..5301
                     /locus_tag="ACG80L_000227"
     CDS             4777..5301
                     /locus_tag="ACG80L_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS5022520.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5317..6816
                     /locus_tag="ACG80L_000228"
     CDS             5317..6816
                     /locus_tag="ACG80L_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS5022521.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6904..7506)
                     /locus_tag="ACG80L_000229"
     CDS             complement(6904..7506)
                     /locus_tag="ACG80L_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5022522.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7610..8566
                     /locus_tag="ACG80L_000230"
     CDS             7610..8566
                     /locus_tag="ACG80L_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS5022523.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8871..9827)
                     /locus_tag="ACG80L_000231"
     CDS             complement(8871..9827)
                     /locus_tag="ACG80L_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5022524.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10001..10561
                     /locus_tag="ACG80L_000232"
     CDS             10001..10561
                     /locus_tag="ACG80L_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022525.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10554..11378
                     /locus_tag="ACG80L_000233"
     CDS             10554..11378
                     /locus_tag="ACG80L_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS5022526.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11388..12005
                     /locus_tag="ACG80L_000234"
     CDS             11388..12005
                     /locus_tag="ACG80L_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5022527.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12023..12706
                     /locus_tag="ACG80L_000235"
     CDS             12023..12706
                     /locus_tag="ACG80L_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS5022528.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13389..14624
                     /locus_tag="ACG80L_000236"
                     /pseudo
     CDS             13389..14624
                     /locus_tag="ACG80L_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            14632..15519
                     /locus_tag="ACG80L_000237"
     CDS             14632..15519
                     /locus_tag="ACG80L_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5022529.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15536..16372
                     /locus_tag="ACG80L_000238"
     CDS             15536..16372
                     /locus_tag="ACG80L_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5022530.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16375..16998
                     /locus_tag="ACG80L_000239"
     CDS             16375..16998
                     /locus_tag="ACG80L_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022531.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            16995..18287
                     /locus_tag="ACG80L_000240"
     CDS             16995..18287
                     /locus_tag="ACG80L_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5022532.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18646..18743
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18859..19440
                     /locus_tag="ACG80L_000241"
     CDS             18859..19440
                     /locus_tag="ACG80L_000241"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS5022533.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19443..20750
                     /locus_tag="ACG80L_000242"
     CDS             19443..20750
                     /locus_tag="ACG80L_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS5022534.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21043..21531
                     /gene="purE"
                     /locus_tag="ACG80L_000243"
     CDS             21043..21531
                     /gene="purE"
                     /locus_tag="ACG80L_000243"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS5022535.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21524..22648
                     /gene="purK"
                     /locus_tag="ACG80L_000244"
     CDS             21524..22648
                     /gene="purK"
                     /locus_tag="ACG80L_000244"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS5022536.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22661..23956
                     /gene="purB"
                     /locus_tag="ACG80L_000245"
     CDS             22661..23956
                     /gene="purB"
                     /locus_tag="ACG80L_000245"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS5022537.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24136..24513)
                     /locus_tag="ACG80L_000246"
     CDS             complement(24136..24513)
                     /locus_tag="ACG80L_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022538.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24769..25497
                     /gene="purC"
                     /locus_tag="ACG80L_000247"
     CDS             24769..25497
                     /gene="purC"
                     /locus_tag="ACG80L_000247"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS5022539.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25497..25751
                     /gene="purS"
                     /locus_tag="ACG80L_000248"
     CDS             25497..25751
                     /gene="purS"
                     /locus_tag="ACG80L_000248"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS5022540.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25753..26424
                     /gene="purQ"
                     /locus_tag="ACG80L_000249"
     CDS             25753..26424
                     /gene="purQ"
                     /locus_tag="ACG80L_000249"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS5022541.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26425..28647
                     /gene="purL"
                     /locus_tag="ACG80L_000250"
     CDS             26425..28647
                     /gene="purL"
                     /locus_tag="ACG80L_000250"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS5022542.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28632..30071
                     /gene="purF"
                     /locus_tag="ACG80L_000251"
     CDS             28632..30071
                     /gene="purF"
                     /locus_tag="ACG80L_000251"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS5022543.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30094..31146
                     /gene="purM"
                     /locus_tag="ACG80L_000252"
     CDS             30094..31146
                     /gene="purM"
                     /locus_tag="ACG80L_000252"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS5022544.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31143..31721
                     /gene="purN"
                     /locus_tag="ACG80L_000253"
     CDS             31143..31721
                     /gene="purN"
                     /locus_tag="ACG80L_000253"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS5022545.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31718..33253
                     /gene="purH"
                     /locus_tag="ACG80L_000254"
     CDS             31718..33253
                     /gene="purH"
                     /locus_tag="ACG80L_000254"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS5022546.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33269..34543
                     /gene="purD"
                     /locus_tag="ACG80L_000255"
     CDS             33269..34543
                     /gene="purD"
                     /locus_tag="ACG80L_000255"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS5022547.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34665..35579
                     /locus_tag="ACG80L_000256"
     CDS             34665..35579
                     /locus_tag="ACG80L_000256"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS5022548.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35712..37277)
                     /locus_tag="ACG80L_000257"
     CDS             complement(35712..37277)
                     /locus_tag="ACG80L_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5022549.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37270..38721)
                     /locus_tag="ACG80L_000258"
     CDS             complement(37270..38721)
                     /locus_tag="ACG80L_000258"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5022550.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38735..39751)
                     /locus_tag="ACG80L_000259"
     CDS             complement(38735..39751)
                     /locus_tag="ACG80L_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5022551.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            39971..41059
                     /gene="chvE"
                     /locus_tag="ACG80L_000260"
     CDS             39971..41059
                     /gene="chvE"
                     /locus_tag="ACG80L_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5022552.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41113..42639
                     /gene="mmsA"
                     /locus_tag="ACG80L_000261"
     CDS             41113..42639
                     /gene="mmsA"
                     /locus_tag="ACG80L_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5022553.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42641..43846
                     /gene="mmsB"
                     /locus_tag="ACG80L_000262"
     CDS             42641..43846
                     /gene="mmsB"
                     /locus_tag="ACG80L_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS5022554.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44103..45080
                     /gene="guaC"
                     /locus_tag="ACG80L_000263"
     CDS             44103..45080
                     /gene="guaC"
                     /locus_tag="ACG80L_000263"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS5022555.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45344..46768
                     /locus_tag="ACG80L_000264"
     CDS             45344..46768
                     /locus_tag="ACG80L_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5022556.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT        14879 a         8030 c        10327 g        13555 t
ORIGIN      
        1 tgttaatacg tattagcaaa ataaaagctt tttttattgt gaataaatga acataattag
       61 gaggagaaag atgaacttat ttgacatttt tcgatcgact ttgacagata tgtcgattgt
      121 cagtgcaatc acttcaacag tttttattat attattaggg tttgtttgta gaaaaaaggg
      181 gattttttct gaatctgttg gaaaaattct atcgaaagtc gtcttaacag tggctttgcc
      241 agcactggca tttcgagctt tcatgcaaga tatcaataca gaattactga tgcaaggaat
      301 caatgtcttg atttggggat tagttattta tattattctt attctagttt caaaaccttt
      361 tttcttaagc tataaagggg ataagcaaac aacgttgcgt gtcctgacga tctttggttc
      421 tacgaccttt ttcggaacgc ctatcgtcag tgcgatttat ggtcctgtcg gcgtgatgta
      481 ttcctcaatt ttcaatattg gctaccgaat ctttttgtat tcttatggat acataaaaat
      541 gagtggcttg aagatggaag ctaaaaatat caaagcgatg ttcgtcaatc caatcattat
      601 cgctactttt gccggccttt tcatttggct gttccaaggg tatctgccac aagtgtcagt
      661 tacaggtgca gaaggagaga taagccaagt agcgtttttg cgaattgacc agactgcacc
      721 gtggctattt cagccgatgg attatttagc tggtttaagt tcaccattgg cgtggctagc
      781 aatcggcgca acattaggtg aagtaagctt caaaaaagct gcatctgaaa aaacttcatg
      841 gtattacagt ctagtcaaag tggtatttgt acctgcattg aatatcgtga ttctagcagt
      901 tttgacggca acgaatattc taccagtcaa ttttgaagca ctggcaacaa tcgttatcat
      961 gatggctaca ccagctgcaa cagtggcagc agcctatgct atcagttttg atagggaggc
     1021 acttttagcc tccaatgcat ccttactgtc aactattttt gctgtggtac tgacacctgt
     1081 atggatcgtt gtattacaaa tgattcatgg cataggttta ttttcataag aaatggagga
     1141 agaaagtatg agaatcgcat gttacggtgt tcgtccgaac gagatagatt tttttgaaaa
     1201 attgaatatt taccattatg atctcagtct ttatgaagaa ctgttgacac atgacaatat
     1261 cgtgtcagca aaggaccatg atgctgtatt gttgagggga aattgtatag cagatgagac
     1321 caatcttgca aaaatgcagg aatatgggat tcgttatgtt tttacacgga ctgtgggagt
     1381 caatcatatc gatctacaag cggcagctga ttttggtatg acagtagccc gtgtaccatc
     1441 gtattcgcca aatgcgatag cagagttatc tttgactttt gccatgatgc ttttgagaaa
     1501 tactgcgtat acgacgatcc gaacatcttt caaagatttt cgagtggatg agcaaatgtt
     1561 tagtcgtgaa atcagaaatt gcacagtagg gatcatagga acaggcagga tagggttgac
     1621 ggaagctgtt ttgtttaagg gactaggagc caaagtgatc ggctacgata ttcatcagac
     1681 agaagctgct aaaaaagtat tgacgttcca ctcgcttgaa gagttattag ccaaaagcga
     1741 tattgtcagt attcatgtac ctcatatccc aggtgaaaac gatcaaatga tcaatgaagg
     1801 atttttagct caaatgaaaa aaggtagcat cttgattaat acagcccgtg gagagcttca
     1861 agataatgaa gctattttaa gagctttgaa aagcaatcat ttagctggtt ttgcaaccga
     1921 tgtttttgca aatgaaacag aggttttttt ccgatccttc aagccatggg agacgattcc
     1981 agaccctgct atccagcaat tagtagaaat gtaccctaga gttcttgtaa ccccgcatgt
     2041 aggttcaaat acagatgagg cattatcaaa tatgatttcg acaagtttcg agaactttcg
     2101 tgaaataata gaaactggaa aaacgaaaaa tgaggtatct cttcctaaag cccgtcaatt
     2161 aagataagaa aacatggata ttcgtcaata aaatgtttaa aaatagtcca agtaaacagt
     2221 tgttacttct gtatacttgg actattttgt atgggataat tcgatgactc aacagtaatc
     2281 tgtgagcttt ttccgcccat tctttgtcaa ttcataacag gttagcggct ggccgatttc
     2341 caaatatttg attctttcgt tcaatatatc ttgttctact aaaaaattca aatatttctt
     2401 gacggatata cgtgacagtg agcttttttg agccagttct tttgttgaaa aaatagtatc
     2461 ttcttttaga ataaatgcac ttattttttt taaagtcagt tttgacagac ctttaggcaa
     2521 atcgatgaat gatggtattt cgtgaatggg agtggtgtgg ttgaatactt gatcgatcaa
     2581 tttctgatcg gctttttcag tcgtttggat tgtctgagag aaagcaagat acttttcgat
     2641 cgccagctga aaacgttgca aagtgaacgg tttgatcaaa taatctaagg caccgtagcg
     2701 gatcacattc tttatggttt cgatatcatc tgcagcagtg atcaaaatca ctgggatatc
     2761 atttccttgc tgtctaagcc aagataaaaa atggatacca ctttcgccag gcatgtatac
     2821 atcaagcagc agcagatctg cgtttttttg tctgaaatat ttttttcctt cttctacatt
     2881 tctgaaactt tcgattacgt tcatattagg gatgctttcc acaaaagtac gattcaattc
     2941 tgcaaccatt ggatcatctt cgattatcag gacatttgtc atcattttga tcttcctttt
     3001 ttggtaattc tagatagacg attgtaccgc catttgtttg tttggaaaat tctaaaaatc
     3061 caccgtggcg tttgacgatt gaatggatga gattcaatcc aaacccacga ttgattccct
     3121 tcgtcgaaaa acctagcgta aatatttttt ctgagatttc ttctgatatc ccgacaccgc
     3181 catcgcagac ggtcacgatg aatacttcag cttctgtgtc atagtttaaa tgaatccgga
     3241 cctgtccgtt tctttcatgt ttttgtagca cggcttcttt tgcattatca agtaagttcc
     3301 ctaacacttg caaaagtgaa tggatggagt catccatctc cattttaggg ataaaagaat
     3361 cgctttctaa cagcatttca acagattgtt ctttggcttc gtttattttg cctaataaaa
     3421 aaccggcaat tgcaggtact ttgatcagac ttgagacttg gccaatctct tttttataat
     3481 tttgatttag ctgctggata aaatgggtga cttcttcata cttcttcaaa tcaatcaaac
     3541 cagaaatgac atgaagttta ttcatgaatt catgggtttg agctctcatg gagtttatat
     3601 attgtttcgt ccccccaagc tcctccatta atttcattac ttctgatcga tttcgtaaag
     3661 ttgcgactgc tcctaaaaaa cggtcattgc taaaaagagg tgtaacgcta gaaatagttt
     3721 ctatcccatt taaatacatg gattgagtcc gaacggtcgt ttgatgctca atcgtctctt
     3781 taaacaaaac gtcatataga ttatcactga tttttttcct acttcgaaat ccaatagctg
     3841 ctctgtttgg tagaaaatgt tttttgcagg tgtattgatc aagaggatag ttttatcaga
     3901 agagatggct aagattcctt catctacttc attttcgata atcgtttttt cacttaactg
     3961 ttctgctatt tcttgtggtt caagtccaag cagtactttt ttcatttttt gggcaagaaa
     4021 aacggcgcct atgatgccta cgctaagtcc aataatcaat gaaaaaacaa tcattttttg
     4081 tccatctttg acttgattgt caatcgtgtt gtttgttaag ccaacacaaa ctgcaccaat
     4141 aatctcttga tcataataaa tagggacgaa atagcggaag ccgaacccta acattccttc
     4201 tttttcagag aaaaattctt gtcctccaat cacttttttc acatcttcat gacttgagaa
     4261 aggaagaccg atgtaacttg ggtctgcgtg agaatatcga atgccttcag ggtccatgac
     4321 aacgataaaa tctaaattcg atgcaatcat gatttcattt gttattcctt gaatcgcatg
     4381 tgtcgggtca ccagtctgca gctttttttg gatgagttta tcattggcaa cgattttagc
     4441 aatgctagag attttatctg acacactaga tcggttcatc gaaagaataa aattcctgat
     4501 caatatggca gaaacaatca aagaaacgaa tgtggagaaa caaacgacaa ggatgatcgt
     4561 gttctggagc tttaatggtt ttttcttaaa aaaaggaaac ataggctacc tcgaaagaat
     4621 ttattgtgaa aattttaaca tgaaaacaaa aatttaacaa attgtcaacg ataaacacaa
     4681 ccttcctata aaaaacacca ctgagtcggt aaaagaagct atacagaatt ttcaaacatt
     4741 ggtatgatag acgtagaaat ggaaggaggc gtgtatatga tagggggaat cgcacttgag
     4801 acgatttatt ggtatacatt gctgatttgt gcaggattgg cagtcctatt gattattttt
     4861 ggagatgtat tcgattttga tggcccgctt gatccgatgc ttattatccc ttggttaact
     4921 ttcacaagct tatttggttt tctaggtgag cgtttaacag atcaatcatc tttattttta
     4981 tttattgcta gtggaggcat atcgatagtg ttggtatttt tgcttaattt ctatatattg
     5041 atgccgatga aacatgccga atcaacgatg tcgtcttctg aaaaaactct ggaaggacaa
     5101 gtagctgttg tgatcacacc gatccctgta cgaggtatgg gagaaattca gttgaaaagt
     5161 gttaccggtt ccattagtcg tccagcttgt ttttacagcc cccaagaaca gacagctgag
     5221 cggggaacga aagtattgat cattgagatc aaggaaagaa tctgttatgt catgccttat
     5281 gaaagcatga taaaagctta aaggagcgtg tgttaaatgg aaataccagt tgtttttatc
     5341 gcgatcgcaa tcgcagcttt gattttactg atgttgctga tcgtctttgt agcgaagtac
     5401 cagacagcaa aaccggatga agcgttgatc atcagtggaa gttatttagg aagcaaaaat
     5461 gtgcacattg atgaaagcaa taacaaaatc aaaattgtgc gtggaggtgg cgcattcgtc
     5521 ttgccagttt ttcaacgttc gaatcggatc agtctattat cgagtaaatt ggacgtctcc
     5581 acccctgaag tgtataccga acaaggcgta ccggtgatgt gtgatggtac ttcgatcatc
     5641 aaaatcggtt catctgtaga agagattgct acagcagcag aacagttttt aggcaagaca
     5701 agagaagaac tggaaaatga agcaagagaa gtattagaag gacacttgcg ctcgattctt
     5761 ggttctatga ctgtagaaga aatctatcaa aatcgcgata agttcagtca aagcgtacaa
     5821 gaagtcgctt ctgtagacct tgcaaaaatg gggttgatca tcgtttcttt cactatcaaa
     5881 gaggtcagag acaaaaacgg ttatcttgac tctttaggga aaccaagaat cgcccaagtc
     5941 aaaagagatg cagatatagc agaagcggaa gctctaaaag aaacgcgtat caaaaaagct
     6001 caagcagaaa aagaatcaca agcagctgaa ttacagcgac aaacagagat cgcagaatca
     6061 ttaaaagaaa aagaactgaa attagcaacc tacaaacaag aacaagatgt tgctaaagca
     6121 aaagcagacc aagcctacaa tttagaaagt gcccgagcac aacaacaagt aatcgaacag
     6181 gaaatgcaga ttaaaatcgt agaacgccaa aaacaaatcg aactggaaga aaaagaaatc
     6241 acacgtcgtg aaaagcaata tgattcggaa gtgaagaaaa aagcagatgc cgatcggtac
     6301 gctaaagaac aagaagcaca agctcaaaaa gtcaaagaag tgactgaagc cgaagcagaa
     6361 cgattccgtg tggaagcact ggcagaagcg gaagccaata agacacgttt agctggacaa
     6421 gcagaagccg aagcagcact agcaaaagga aaagcagaag ccgaagcaaa acaaaagatc
     6481 gccaatgcct tcaaggaata tggcgaagca gctgtcttga gtatggttat cgatatgcta
     6541 ccgcaattga tgcgcgaagc agcacagccg ctaggaaata tcgaaaaaat ctctgttgtc
     6601 gatacaggaa gcagttcagg agaaaccagt ggagcgaacc gagtgacaaa ttatgccaca
     6661 aatttgcttt cttctactca agaaacacta aaagcgacaa caggacttga cttgaaggaa
     6721 atcgtagaga atttgtcgac acgtgggact actagccaag taaatatgta tgaagcgcca
     6781 aaagaggcag aacaaaactc gatgaaggaa gaataaagta gtacacttca ccgttcaatc
     6841 aaaaatggag gaagactttt taaagttctt ctctccattt ttttatttta ttgcaaaaga
     6901 aaattagtcc tcgctatttt tcttttgttt ttggatctta gcttctatct cttgtaaatt
     6961 tgttcttagc aatcgttctt tcttttggag ttcgaccaaa tcttctgata gttcttcctt
     7021 cttttctgtt tcaggaggac gaagaaagaa attgataccg attgcaatga atgtccctat
     7081 aaaagtatct agtacacgtt ggatagcata caaagcggat tcgccttgag ggatgctcaa
     7141 cgcaatcagc aacaacgtag ctattgcgga aatgataccg gaattattgt taataccatc
     7201 tgacacgacg atcacaataa taactagaat aggaagcaag atcaattcta ccaaaaaatc
     7261 attgtgaaaa tagcttttga caaaaaaata aatgatagct aatgtaccac ctaatgtatt
     7321 gccgatgatc ctagactttc caaaagaaac ggttgtagtc aagtcctgac gcaaagaaaa
     7381 aacagcagat aatgcagcaa tcatcgggat tcctcgttga gtcaaatgaa aaaacaagat
     7441 acataaaaaa acagctaagg cacttttgat cgtacgcata cctagtcgaa aacggccaaa
     7501 ttgcatagag attctccttt ttggtctttt tactatcata cctaaacttt tgtctgatag
     7561 ctagtttttc attattcaag tataattaaa ataaaaaagg gggaagtaaa tgcgtatagg
     7621 aatgcgaaca gtcaaaacag tgatcagtgt cttcttttcg atggtcacag cgggtagctt
     7681 atcgctgttg tattggccag ctgcagggat cattgcatta ttaagtgtag gaaataccaa
     7741 gcgctctaca ttgcgtacgg gcatgaatcg aatcgcagcc tttatattag ctacggcaac
     7801 agcactgttc agtttttttg ctgttgggta tactattttt ggcttcagtc tttttttatt
     7861 cctgttcatc ccgcttgcct ctcgcttcaa attaaccgaa gggatagtag taaattcggt
     7921 tttggtcact cattatttag tggaggaaaa catgtcttgg cagctgatcg gcaatgaagc
     7981 gggattaatg gcgattggat tagtatttgc tttgcttgca aatgtttaca tgccagatcg
     8041 tacgaagcag ctgaaggaaa accaaataca gatcgaaaaa gaatttcgaa atattttgag
     8101 acaaatggca gaatttcttt tatcagaaaa taaagaagat gtgcagatca aatgcgaaca
     8161 tttactgaca ttcattcgcg aaagccaaga agatgccaga gaacatcaag aaaactactg
     8221 gctcagacag ccgctatact acgaaactta cttctctatg agaagagcac aggccaatgt
     8281 gattaaagac atgctggaaa atctagagag aatccagcaa ccagcttatt atggaaaaca
     8341 tatctatggt ttattgattt atactgcaga aacattttca gagtcaaatg atggcagaca
     8401 aattttaaca cgtattgaag aagtttatgt attgtatcgg caaatgcctc tgccgactag
     8461 tcgccctgaa ttcgaggatc gagcagaatt gttccagttt ttgcagtctt ttaaaagttt
     8521 tattgaaatc aaggcagaat tctcccaaca aaaaatccaa aaatagaggt tgtgaattca
     8581 gctaatattc aggtcaaaaa gctgatttca caacctcttc tttaaacggt gttctcctgt
     8641 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg
     8701 ctccttgtga ctcggagctg aacggtaggc tcacaacctc tttcttaaac ggtgttcaaa
     8761 agctttcctc tgcggtaata agtccagcca aacgaaaaat aggaggagct atttttgttt
     8821 ggctgttctt attacttgat gcagaacagc tttttcacaa ccttttaaaa ttacatattt
     8881 ttccatagaa cttgcatttg atccaagatt tcgatgacat cttttgtcaa gaagagagag
     8941 cgattgggca agtcaccttc aatcatgtgt accatattct ttatttcgta attcattgct
     9001 tcagatgtat tcccgctttc gatgaattcc tttgtaccat ctgtaaataa gatctccgca
     9061 cgatcagcac gcgggtaatc agcaatcgtc acataaccgt tctcaaaggc aacgataccg
     9121 acttttggca tttttgcctg aaaagtcaag ctgacagcag caagttcatt ttctttgtta
     9181 cgcaagatag tgacggattg ttcgtccacg cctgtttcaa aaggaaccat tgtagaggca
     9241 actacttcag gttgagagct caaaaagaaa cgagcgaaag atacagcata cgtaccgata
     9301 tccaacagag ctcctccagc taattctgga ttgaagaacc ggtttttagg gtctggttct
     9361 ttgtaactgc cgaaaggtgc ttggatcatt tttaaagccc ccagttttcc tgtatccatt
     9421 attgagcgta actgctgata aagtggcatg ttgaagattg tcatcgcttc tgctaagatc
     9481 aaattctttt cttctgctaa gcgcatagcg tcagccagtt cttttttatt catcgtgatg
     9541 gctttttcac atagaacgtg tttgcctgct tccaatgctg ccaagatatg gtcgatatgc
     9601 tgtctatttg gtacagcaat gtacacgatg tcgatttcag catcgtctaa catttcttga
     9661 tagcttccgt aagcttttgg aatattgtaa cggtgagcga agttttccgc tttttctgaa
     9721 gttctggaag caactgctgt tagttcgctc gtctcttgat tgaagctttc ggcaaactca
     9781 tgtgcgatgg taccaagacc aacgattccc cattgatatt ttttcatctg aaaacgctcc
     9841 ttcatagttg gattgagttt cttgtctgtc tattattata acaaaaatca cgaagaataa
     9901 agaggagagg acttgaaaca ttaaaaaaaa tgaaagcagc gatttaagag gtgctttttg
     9961 atcaaaactt aagtagaata gttaaaagga ggggataaac gtgcatcaag tatatattga
    10021 tcttattatg agtgtattat tagaacagtt tgaaactgaa aaaaattttt tagatgaata
    10081 cttgctgatc agtccagaac aatggcaaaa atggaaagat ggcgagaaaa atctgccatc
    10141 tgaagcgatg cagaaagtaa aaaatctttt cactgattat gaatggatgc taacgcaaaa
    10201 gattttgcgt caaacgatta tttttcccga aaaacgaaac gttgccgttg cagagtacaa
    10261 gcaaatcaaa acacgcatcg cgcaaaaatg gcttagcagc aaagcagcaa aagtcgagct
    10321 gatccctttg aaaaataatc agcaggctgg tagatacctt gacttgaaag taagtattca
    10381 atatgatgaa tggggctatg atgatatttt gaattttcgt ctgccagcag atgttcaaaa
    10441 acagatcgaa ggagaaaaaa tcgaacttct cgattgggtg aatgaaaatc tggaagaaac
    10501 ttacgttcat gaggcgaatg gggaacgtga agaacaacga aaggattttt caagtgaata
    10561 aatttttagg atttggagca gttgtgatta tgatcgcagc aatgggctat gttattttta
    10621 gcgatgatga ttcgcatgtt caagccggca atcaagcagt cgaacgaaca agcaccaaaa
    10681 caactgctag taaagaaaag aacagtcaaa caaccagcag caaggtaaag gacaaagaaa
    10741 caaaagatcg tacgtccaaa caattgccag atgttaaaac agacgattgg aagctagttt
    10801 tggttggtcc agatcacaaa ttagaaaaag aaattgatga gtctactcaa ttggcatcat
    10861 taagtaacgg atacttgatc gacaaacgca ttgctccaga atatgaagca tttgccaagg
    10921 ctgctgaagc agctggattt ccattagtga tggtctcagc ttaccggtct gtatcatccc
    10981 aacaacaagt tttcgaacaa aatgtccaag atgtgatgag tcgtcaaggt gtatcagaag
    11041 aggaggcgac aaagattact aaagaaacga ttacggagcc aggatacagc gaacaccaca
    11101 caggtctggc agtcgatgtc gtcgatcaaa actggtacaa cagttatcct tctactgtat
    11161 tggatgcaag ttacggagat cagcctggag ctaaatggat cgcagataat gctgcgcagt
    11221 atggatttat cgttcgttat ccaaaaggac acgaagatat aactaagatc acatatgaac
    11281 cttggcactt acgttatgtc ggtaaagaaa acgctgagta tatcaccaaa caccaattga
    11341 cgctagaaga atatctgaat gaattaaatg aaaagtagga attgatcatg gcgaaaaaaa
    11401 agtggaaaaa gcaaaagcgc aagaatatcc gttggccagc agtggttgcc ggtgtagcag
    11461 tgatactctt agcctttgtt ttttccttga agaatctgtc ttctcctttt actgataatc
    11521 agcaagaaaa tgaagcgcaa tcccatcagc aatttattga acggcttgtt ccgcatgccc
    11581 aagaactaca agatggttat ggagtgcttc ctagtatcat tttaggacaa gctatattag
    11641 aatcaaattg gggaaaaagt accttggcta gcaaatacaa taatcttttc gggatcaagg
    11701 cttatgggga tcagaagaaa gtaagcctgg aaacaaagga atttgttaac gaagaatgga
    11761 taactatcca aggagacttc aaagtgtatg attcatggga acagtccatg gatgaccata
    11821 cacaactgtt cgtccaagga gtagattgga atcctgcgct gtatgaaaaa gtgattacgg
    11881 ccaccaacta tcaggaagcc gcacaagcat tgcaagatgc aggttatgcg acagaccccg
    11941 gttatgcgca aaaaatcatc caggtcatcg agacgtatca attgaatcag tatgaccgga
    12001 gatagcaaag gagaagtggc tgatggcaga acaatttacg ccagaaatca tgacggaatt
    12061 gagaaaagat atcgtaagag taccggaaat cattttagaa gcaagcggga tcaagatatt
    12121 tggaaaattg atccgttcga tcatctttac tacggatatt gcgatcatac gtaatacaaa
    12181 tgcgaatgca gtaattgctg tgtatccttt tacacctcat ccagcgatta ctaaaagcat
    12241 tattgaggca gctgacattc cagttttctc cggtgtaggt ggtggtttga cacaaggtgc
    12301 tcgttcttcc tacatgagca tgttcgcaga agcgcaaggt tcgttagggg tcgttttgaa
    12361 cggtcctacg cctttagaga cagtaaaaga agtttgtcga gtagttgata ttccagttat
    12421 cagtacggta acgtcgaaat atacagaaat agatgaaaag ttagcagctg gtatttcgat
    12481 tatcaatatc agtgcaggta aagaaactgc agaagcagta cgccattttc gtaaaaaata
    12541 tcctgaactt cctatcattg cgacaggtgg tccaacggaa gaaagtattg ctgagaccat
    12601 tcaagcagga gcaaatgcca tcacatatac gccaccaagt agcggagagc tttttagtcg
    12661 taaaatggaa aaatatcggg aaactgagaa aaagaatcat gaataacagg tgagaatcaa
    12721 aagacgagga agagcagata gtggctcttc ctcgtctttt gtactccagt actatttgtt
    12781 cagaattcaa aaaaaagaga aatttttttg tttttttacc tgtaatgtac ataagaagag
    12841 ttaaaacctt ttgttattat tagcattcta acaaaaaaat gtcttgattt attgaagcgc
    12901 tttctataga atgaaaaacg aataaaaact aaaaaaggca cccgacaatt tagtagcagc
    12961 taaattgtcg gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc atagtcaatg
    13021 cttgtgcatc gacgtgtata ttgtacctaa tttcatacag aattactagt tatttttctg
    13081 ttttttaggg aagagcgcac acgaagaagc atgcttattt agctatgtca aggatattgg
    13141 gcagttgtct gatatccttt tttctttgcg taattatcct cccatcattg aagcaagaag
    13201 tgtttttagc gatgaaagaa gaatcagcac tatctaattt ttcttgaaag tattgagttg
    13261 ttcatcctag gcagaacgat aaggcaagtc ttggttttta ttccccttga aaaagatcgt
    13321 ttaataaata tgttctgtat aaaaatacag tatattgaaa aataacaatg gaagtagagg
    13381 tgcagagaat gaagaaaaga atgagaatca tcttagcttt aagtgttatg ttattcttct
    13441 tgacggcttg tgggcaagaa gatgacaaaa aaatcacgct gaacatggct tggtggggtt
    13501 cgcaagtccg acacgatgca acagtaaaag taatcgagct ttatgaagaa aaaaatcccc
    13561 atgtgacgat cgattatgaa ttttatgatt atgaaggata ttacacaaaa ttaggtacac
    13621 tagcagcatc agatgatatc tgggacattt tccaactgaa caatcaattt cctcaataca
    13681 tcaatcagat tgaaccatta gatgaatatg tggagcaagg gataattgat atttctaatg
    13741 cagatgaatc ctatttagca acgacgactt acgacgggca attagttgga ctgtctaacg
    13801 gggtgaattc gtttgcgatt gcttacaata aaaatatatt tgaacaatta aatatccctg
    13861 aaccagcttc aaattggaca tgggatgaat ttgaacaaat cagctatcaa atcactgaag
    13921 aattaggaat ctatgctatt tcaagattcg aagattttat agctggctgt attattcaaa
    13981 taccacaagt tgagaaagga ttgaattttt ataatcggga agatctatct aacagcttgg
    14041 gatttgaaaa tccagactat ctaacagatt ttttccagct gcgtaaaaat ctggtagaca
    14101 aaggggccca tccagaacca ggtggtggag ctgcaagtac gacggatctg tcttttcagg
    14161 cggttaggga tagagaagcg gcaatggtgt ttctatcaag taaccagttg acagaaatat
    14221 tgtcaggggc tcctgaagat atggagattc agttgattaa tccacctcgc agaaaagcag
    14281 atggagaatc tggtatccct ttacgttctt cgcaaatgct gagtatggca aaaagctcag
    14341 cccataaaga agaagctgct aagtttattg atttctttca aaacagtgaa gaagccaatg
    14401 aaattttaaa aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc
    14461 gtgcagatga tgcaactttg caagcgacat ttgattattt agaaatgatt tccgatatgg
    14521 acaacggcga agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa gaccaataca
    14581 atcttttagt tgaaaaagtc atttatgaag aagtaacagc agaaggaagg catgaaaaaa
    14641 gaaaaactaa aacggttctt ggatcaagac aatgtggtcg gttacgtatt tgcttctcca
    14701 ttcatcctag gatttctatt gataacagtg ataccgatga tcttgtcact ttattattcg
    14761 ttcaccgatt atcgtttggg aaatccagtc tcatggatcg gcttggataa ttatataaag
    14821 atgctgagtg atgcccgttt tttgaactcc atccgagtca cttttctcta tgtatttgca
    14881 tctgtacctg tgaaactgat ctttggtcta ttgattgctt atcttttaac gaggaatatc
    14941 aaaggaacga ctttctttcg ttctttgtat tatgtgccta gtttgatcgg tggcagtatt
    15001 tctatcgctc tagtttggcg ggagttgttt tctaaaaacg ggttagtgaa tattttcctg
    15061 gaacgattag gcgcacctgc gctttcctgg tttagcgatc aaaaactggt catgatccct
    15121 cttgttttga tgtctgcttg gcaattcggt tcggcaatga tcatctttgc tgcaggtatc
    15181 aaagagatcc caggctcgta ttatgaagcg gcagagatcg atggagcgaa caaatggcag
    15241 acgttttaca agatcacgtt acctcttctt tcgccgatca ttttgtataa cttggttatg
    15301 cagacaatcg cggcgtttat gacttttacg caggcattta tcattaccca aggtggtccg
    15361 aatgatgcca cgaactttta tgctctttat gtgtataacc aagctttttc ttttaattct
    15421 atggggtatg ctagtgctat ttcgtggatc atgctgttga ttatgggagc tgtcaccttt
    15481 ctgattttca aatcatcaaa aaaatgggtc gttgtgtgaa tgagaggagg aagaaatgaa
    15541 taatacagta acgaaatggt ttcaatacgg ttttattttg ctattaggtc tagtcatgat
    15601 ttatccggtc ttatggatga tcgcaggttc ttttaaaacg gatcaagaga ttatgagtgg
    15661 ttcgctgaat ttgattccag agacttttca atgggaaaat tacgcaaaag gatgggcggg
    15721 gtttgctgga atttcatttt ttactttctt taggaattcg tttgtcatct ctgcagtttc
    15781 gacgataggg acagttctga gctcgacttg tattgcttac gcattatcga gaatcgattt
    15841 caaaggaaaa agattctggt tcgtcgtcat gttagtaacg atgatgattc ctgcacaagt
    15901 gattttgatt ccgcagttta ttatttacaa tcggctgaat ttggttggga cgtatgtgcc
    15961 gctgatttta ccacattttt ttggacaagc atttttcatt taccagatca tgcaattcat
    16021 ggtcagcatc cccaaagagt tagatgaatc agcgattatt gacggatgca gcaagtatag
    16081 tgtctttacc aaaattattt ttcctttgct gaaaccttcg atcatcacga cgatcatcat
    16141 ccagttttat tggaaatggg acgacttcat gggaccattg atttatttaa acaagccgag
    16201 atcttacacg gtatcgattg ccatcaaatt atttgcagac gctggatcta gtacgactta
    16261 cgcgagtatg tttgcgatgt ctacgttgtc gctgctaccg gtttttctga ttttcttatt
    16321 cttcaataaa tatcttgtcc aggggatcag tacaagcggt ctgaagggct gaatatgaaa
    16381 aagccaatag aagagatgtt tctttacaag ctcaactact atgtctgtcg gattttttta
    16441 accagtctta tcagtaatgc tcttttcgct ttagttctag gatgtttgtt cactttagga
    16501 ttagtgctgt ctgctcgtgt tgtctatctg atttattttt tgatgatgta tccttcttat
    16561 tatctatctg tccattactt ggcagaaaaa aaggtatgtt ggaggaaagg gaaaatcaaa
    16621 cggatgtgtt tcatcattac gttgtttcag ctgtttgtca ttatttttgc agttgattgg
    16681 tatttattag tgggaaagat tggagcttgg tatctgattc cttttctatt gtgtctgttg
    16741 ctttttgtat cccatagttt ttttagtacg ctaattgata tctgcaaaga agataaagca
    16801 gatagtcttg tttatttgat caaaaggaat atctggctgt tgtcaaaaaa cgtcaagggg
    16861 tttctgtggt atttatgcct tttgtatgga ctgttctggt gtatacagcc tgatttttta
    16921 ctcactgcat tactgtatcc agggctgcca tttgtcacag tattttgctt aaaagaaaag
    16981 gagaatgact ttaaatgaag aaaaaatatg tacaagtcgg acttggtggg agagcgcgtt
    17041 ttttttatga ggcaattgcc tctacgtatc atcaaacttg tgaactagtc ggtttttgtg
    17101 atgtatctca aacaaggatg gattatgcaa atcggatact tgtgaacaag tatggacaga
    17161 aaaaagtaaa gacatgtatg ccatcacaat ttgagcaact actggatgac gtacggccgg
    17221 ctgaagtcat tattacgtca gtagatcgaa cgcatcatga ttatatcatt cgtgcaatga
    17281 aaaaaggata tgatgttatt tgcgaaaaac ctctgactat cgatgaggaa aaagctcaag
    17341 aaataataac ggtccaaaaa cagacgggaa gaagcctgcg agtcactttt aattatcgtt
    17401 atgcaccgta tcacacgaag ataaaggaac tgatagaaag tgatgtgatt ggtgatgtgt
    17461 tttctatcca cttcgaatgg ctgttaaata cgcaacacgg ggcagattat ttcagaagat
    17521 ggcatcgaaa taagcacaat agcggcgggc ttcttgttca taaatctacc catcattttg
    17581 atctagtgaa tttttggttg aacagccgtc cccaaaaggt atttgcattc ggtggattaa
    17641 acttttatgg gatgcataat gcggagaaac gcggagtaga aatattttac agtcgatgct
    17701 atggaagcga actagctaaa gctgatccat ttgctataga cctgtcagcc aatgaggaat
    17761 tgcgagaaat gtatttagaa gcagaaaaag acagtggata tattcgtgac cgcagtgtat
    17821 tttccgatga tatctctatt gaagatacac taggcgtaac ggtcaaatat gaaactggtg
    17881 cgattttgac gtattcgcta aacgcgtata tgccgtggga aggattcaat gtttctttca
    17941 atggcagcaa aggacggatc gaaatgaaag tgatcgaaaa gtcttacatc aatgcaggtg
    18001 gagataaaaa ggatgaagga gcagccgttc ttcagtccat ccatgtctat ccgatgttcg
    18061 gtgagccata tgaggtaatc gtcgaacaag aagcgggcgg acatggcgga ggagatccgc
    18121 gtttattaga tgatctattt ggtgaagaaa aagaagaaga tccgtggaat cgggcagcaa
    18181 ctcacgtaga tggtattttg tcgattttga caggaatcgc agcaaatcat tctattgctt
    18241 caggaaaagc agtagtgatt gacgagcttg tcagcttaaa agaataagaa tagatttacg
    18301 gaaattactt cccgtagttt ataaaatctt gccttattgc cgataggctg tcttttgaac
    18361 actatttatt cggaaaaagg ggctatgaca tgctttttgt catgccttct ttttatgtgg
    18421 gctgcttttt tagataggag aaaataaaaa taactattta aacccgaaca aaaaggaata
    18481 atgcattaaa aatcattaga ttttacaaaa aaataagtaa accacgttgt cagtttcaga
    18541 aagtagtgtt agactgtttt caatatttga aacaacattt tttattcaaa tatggcaaac
    18601 tgccttcacg tgcaaaacag ttgcaagaaa atgaatttac actattatta gttttacata
    18661 cctacttata tatcgtcata atatggatga cagtttctag ccagtaccgt aaatgctgga
    18721 ctataagtaa aagattggct atttcaccct tcttttctta tgataaagtg gagggtgttt
    18781 ttctttaaca gaaacatagt aggtttttgc acaaaagcgg ttatttcggt attaataaat
    18841 gataaaggag caaataaagt gaaagaatta gttgaacgta ttcaaaaaga cggacgtata
    18901 ttaggcgagg gcgtattaaa agtcgacagc ttcgtcactc accaagtaga ccctgtattg
    18961 atggaacaaa tgggaaaacg gtttgcagaa gtatttaaag aacaaggaat cacgaaagtt
    19021 gtgaccattg aagcttctgg gatcgcacca gctttatttg ctgcaagaga attagatgtt
    19081 ccaatgatct tcgctagaaa agcaaaaagt ttgacgatgg atgaagaatt attgacagct
    19141 tctgtttatt cttttacgaa acaagtgaca agtacgattt ctatttctcg taaattttta
    19201 tcttcagaag acaaagtatt gattatcgac gatttcttgg caaacggaca agcggcaaaa
    19261 ggattgatcg aattatgcca gcaagctgga gcgcaagtag aaggaatcgg gatcgtcatc
    19321 gaaaagtctt tccaagatgg acgtcagtta cttgaagaaa tgggattgag agtcgtttct
    19381 cttgcgcgta ttgcttcctt gacgaatgga caagttgagt ttcttgagga ggacgcataa
    19441 tcgtggaaaa agaaacacaa aatggaaaag ctgcggtttt agggctgcag catctgctag
    19501 cgatgtatgc aggagcagta gcggtgcctc ttttgatcgg tacaggacta ggctttaatc
    19561 aagaacagat gacttatttg atttcaattg atatctttat gtgtggatta gcgacattat
    19621 tgcaattgac agttaaccga tttttcggaa ttggcttgcc tgtcgtttta ggatgtgcga
    19681 tccaagcagt agctccactg atcttgatcg gaaccaatga aggagtagga gctatctacg
    19741 gatctattat cgcttctggg atctttgttg tgttagtttc tggtgtcttt tctaaaatta
    19801 aaaaactatt ccctcctata gtaacaggaa cggttatcac cgttattggt ttgactttga
    19861 ttcctgtagc aatcgagaaa atgggtggag gtaatgcaac agcagctgat tttggtgaca
    19921 aaacaaatct gctacttgca tttgtgacga ttctcctaat tgtaggcgtg caaatgctgg
    19981 caaaagggtt cattcgttca atcgcggtat tgattggttt agtcggagga agcatcttag
    20041 ctgctttcat gggaatgatt gatctgagcg cgattggaga tgcgccgttg ttccatgttc
    20101 cacaaccctt ttattttggt aaaccaactt tcgacgtatg gtcgatttta ctaatgatca
    20161 tcatttccat ggtcagcatg gtagaatcaa caggtgtata ttttgcactt ggtgacatta
    20221 ctgggaaaaa tgttggagaa gaagaattga aaaagggata tcgtgcagaa ggacttgccg
    20281 ttatcttagg cggtattttc aatacattcc cttatactgg cttttctcaa aatgtcggat
    20341 tggttcaact ttcgggaatc aaaacaagaa aacccatcta tttctctgca ttcttcttga
    20401 ttgttttagg attattccct aaaatcggtg cgttagcaca aatcatccct gagccggtac
    20461 ttggtggagg aatgttagtc atgttcggga tggtggcagt ccaaggaatc cgtatgctgt
    20521 tggaagttga tttcagcaat gataaaaatc tattgattgt tgctgtttct gttggatttg
    20581 ggctgggttt caatgttacg ccgacattat tcagtcaaat gccgcaaaca ttgcaaatgt
    20641 tcactggaaa tggcatcgta atgagtagca ttacggctat catattgaat ttgatcttta
    20701 atggattgaa aaaagatgaa gaaatattgg aagaagcctt caagaagtaa tagagcaaaa
    20761 gaaaggttgt gaaatcagcg ttttgacctg attgttagag aaacaaacgg aaaaatagtt
    20821 cctcgtattt tgccgtttgt ttgagctaat accgagggtc agctgattga acaccgttta
    20881 tccataaaaa ggaatgtgac ctaacttttg tcacattcct ttttttcatc tttaataaac
    20941 acgaacaata aaatgttttt ctttgttaac attcgaaatt tctttgacat caaaaaaagt
    21001 gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa caatgacagc aaaaatctct
    21061 gtgattatgg ggagtacttc agattgggaa acaatgaaag aagcatgtca aatattggat
    21121 gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc agatttgatg
    21181 tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc aggagcaggg
    21241 ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa ctttgccggt tatcggtgta
    21301 ccggtccaat cacgaacatt aaatggtttg gattcgcttc tttcgattgt ccaaatgcct
    21361 ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa tgctggacta
    21421 cttgcgatac agatgctttc catgtatgat ttagaattgg aggctaaatt ggctgaaaga
    21481 cgttcaatgt tggctaaaac tgttatagaa agtagtgatc aacttggtta aacctttgtt
    21541 gcctggttca acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga tggcgattag
    21601 tgcaaaagaa atgggcttta aagtaggtgt gctggatcct gtgagcgatt gtccagccgc
    21661 acaagttgca gattggcaca ttgaaagtgt ctatgatgac acctttgcgc tgaaagagct
    21721 tgccagaaga acagatgtca ttacctatga attcgaaaac gtcagtgtag aggcgttaaa
    21781 tgcgatttta cccatgtcat ttattcctca agggacagac ttgcttgcga ttacgcaaga
    21841 ccgcctgtta gaaaaatctt ttttggaaac gaataacatc gtgattgctc cttatgcaac
    21901 gatcgtcagt ccgacggata tccaagatgc gattgacggg atcggctatc cttgtgtttt
    21961 gaaaaccact cgaggcggct atgatggcaa ggggcagtat gttttgaaaa gtcgggcgga
    22021 tctagctcct gccatggacc ttttaagaga aggaacctgt gttttagaag catggatccc
    22081 gtttgaaaaa gagatttcga tcatggtggc aggaaatggt caaggagatt tcacgacttt
    22141 tccggtcgtg gaaaacatcc accacaacaa tattcttcat gaaacgattg ctccagccgc
    22201 gattgatcag gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg cagaagcagt
    22261 gagcctttca ggtgttttag gggtagaaat gtttttaaca aaaacaggcg ggctatatgt
    22321 gaatgaatta gctcctcgtc ctcacaattc tggacattat tctattgaag cttgttctat
    22381 gagtcagttc gatgcccata ttcgaggtat ttgtggttgg ccattaggag aaaaagtacg
    22441 tttgataagc aatgcagtaa tggtcaatgt tttgggagaa caactgctgg aaagctatcg
    22501 cttgattgag aataagccag actggcaatt ccatttctat ggaaaaaaac aagcaaaaaa
    22561 aggtcgcaag atgggacata tcactatctt gacagaagac atccagcaaa cgctggaaga
    22621 aatcaaacaa acgaatattt gggattagag gagaaaaaac atgttagaac gttatacacg
    22681 ccctgaaatg gggaatatct ggacggaaga gaacaaatac cgtgcgtggc tcgaagtcga
    22741 gattttagca gatgaagctt gggcagaact tggtgaaatc ccgcaagaag atgtgaaaaa
    22801 aattcgtgag catgcatcat ttgatatcga tcggatcttg gaaatcgaaa aattaacacg
    22861 ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca ttgggagaag aaagaaaatg
    22921 ggtccattat ggtctaacaa gtacggatgt cgtagatact gcttatggct atctcatcaa
    22981 gcaagcaaat gatattttac gggaagactt gaaacgtttt acggaaatca ttggcgaaaa
    23041 agcaaaagaa cacaaatata cagtgatgat gggtcggaca cacggtgtcc atgctgaacc
    23101 aactactttt ggattgaaac ttgccacatg gtattcagaa atgaaacgga acatcgaacg
    23161 atttgaacat gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag tgggaacgtt
    23221 cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa caattgggga tccgtccaca
    23281 agaaatctct actcaagttc tccctcggga tctacatgct gaatatttct cagcgatggc
    23341 gcttattgct acaagtattg aacgttttgc aacagagata cgcggattgc aaaaatccga
    23401 aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg ctatgccgca
    23461 caaaaggaat ccgatcggtt cagaaaatat ggctggtcta gccagagtgg tgcgcggaca
    23521 catggtcact gcctatgaaa atgtcacatt atggcatgaa agagatattt ctcattcttc
    23581 agcagaacga atcatcttgc ctgataccac gattttattg aattacatgt taaatcgttt
    23641 tggcaatatc gttaaaaatc taacggtctt tcctgaaaac atgaagcgga atatgtctgc
    23701 aacatttggt ttgatttata gccagcgcgt tatgctgaaa ttgatcgata agggaatgac
    23761 aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg acaaccaagt
    23821 gcagttccgc ccgctgttag aagaagatcc aaccatcaca tcggtattat ctaaagaaga
    23881 aatagacgat gcctttgatt atcatcatca tttgaaaaac gttgacgtga tattcaaacg
    23941 agtaggtttg gaataaagaa gagtgagttg caaaattcaa ttaaaataga atagcaaaaa
    24001 taaccaaaaa agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct
    24061 tatcaataat aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata
    24121 caaaaaatag aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg
    24181 gagatgttgc agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat
    24241 ctggatacga taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc
    24301 gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt
    24361 tgctcggcgg atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc
    24421 tttgatcgac agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa
    24481 aaaaagctga ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat
    24541 ccaaattttt agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat
    24601 ggtataagat tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg
    24661 tacatatttt tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac
    24721 aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa
    24781 gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt
    24841 tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt
    24901 ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga
    24961 ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta
    25021 caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt
    25081 ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat
    25141 gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaaa
    25201 ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa
    25261 ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat
    25321 ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt
    25381 acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc
    25441 tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt
    25501 atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag
    25561 taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat
    25621 actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt
    25681 gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg
    25741 aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat
    25801 atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag
    25861 tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat
    25921 ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct
    25981 gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga
    26041 cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca
    26101 ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga aataatctat
    26161 cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta
    26221 aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac
    26281 attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt
    26341 gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa
    26401 aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc
    26461 aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa
    26521 gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg
    26581 agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga
    26641 cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta
    26701 gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga
    26761 gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt
    26821 gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta
    26881 gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc
    26941 ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta
    27001 ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc
    27061 atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa
    27121 gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg
    27181 gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata
    27241 ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa
    27301 gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca
    27361 ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga
    27421 gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt
    27481 aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg
    27541 ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct
    27601 cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa
    27661 gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact
    27721 tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg
    27781 agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg
    27841 tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt
    27901 gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa
    27961 ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg
    28021 ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca
    28081 atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact
    28141 actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat
    28201 tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac
    28261 tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga
    28321 ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca
    28381 gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt
    28441 gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa
    28501 gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa
    28561 atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag
    28621 gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg
    28681 tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc
    28741 gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag
    28801 atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag
    28861 aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc
    28921 agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga
    28981 tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact
    29041 ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata
    29101 aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa
    29161 cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga
    29221 aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt
    29281 ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg
    29341 attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca
    29401 gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga
    29461 atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt
    29521 tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa
    29581 aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg
    29641 ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg
    29701 atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag
    29761 gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg
    29821 ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca
    29881 aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga
    29941 tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt
    30001 atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat
    30061 cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca
    30121 aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc
    30181 aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat
    30241 ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg
    30301 aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg
    30361 atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta
    30421 gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga
    30481 tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac
    30541 tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta
    30601 ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt
    30661 cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta
    30721 tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa
    30781 atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat
    30841 atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca
    30901 caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt
    30961 gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca
    31021 gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt
    31081 tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa
    31141 cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt
    31201 atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag
    31261 catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag
    31321 aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga
    31381 tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt
    31441 atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg
    31501 gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg
    31561 accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg
    31621 acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag
    31681 tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt
    31741 atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat
    31801 catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat
    31861 cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa
    31921 aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac
    31981 ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat
    32041 tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag
    32101 tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga
    32161 ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca
    32221 aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata
    32281 cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa
    32341 acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct
    32401 tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa
    32461 taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt
    32521 agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc
    32581 gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa
    32641 tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat
    32701 tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt
    32761 actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt
    32821 aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt
    32881 cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagcgtttg cttggagagc
    32941 tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat
    33001 cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga
    33061 gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga
    33121 atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca
    33181 agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca
    33241 ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg
    33301 tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc
    33361 gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga
    33421 tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc
    33481 tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt
    33541 cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat
    33601 ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc
    33661 agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg
    33721 gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt
    33781 gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg
    33841 agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc
    33901 gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc
    33961 ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt
    34021 aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc
    34081 gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga
    34141 tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga
    34201 tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt
    34261 tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt
    34321 tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt
    34381 tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca
    34441 gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga
    34501 tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt
    34561 ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga
    34621 gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat
    34681 taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg
    34741 cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc
    34801 atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa
    34861 catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga
    34921 tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga
    34981 aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg
    35041 aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca
    35101 gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag
    35161 ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca
    35221 atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc
    35281 atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta
    35341 tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc
    35401 aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag
    35461 tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta
    35521 atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa
    35581 gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg
    35641 ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg
    35701 gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga
    35761 gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt
    35821 tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt tgctaattcg
    35881 attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta
    35941 ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga
    36001 tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc
    36061 cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg
    36121 taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt
    36181 tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat
    36241 aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac
    36301 ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca
    36361 gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta
    36421 agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg
    36481 attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat
    36541 tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa
    36601 tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc
    36661 tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg
    36721 acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat
    36781 tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa
    36841 tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa
    36901 tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta
    36961 agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat
    37021 ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc
    37081 aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata
    37141 attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa
    37201 gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg
    37261 ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag
    37321 tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc
    37381 gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga
    37441 atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat
    37501 cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgcgg taatacgaat
    37561 aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg
    37621 caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc
    37681 aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc
    37741 tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag
    37801 tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc
    37861 atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact
    37921 ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc
    37981 cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg
    38041 aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc
    38101 ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag
    38161 aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt
    38221 gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt
    38281 gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat
    38341 attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc
    38401 taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa tatcattcct
    38461 aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa
    38521 gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg
    38581 gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag
    38641 tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt
    38701 tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag
    38761 ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc
    38821 ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag
    38881 gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt
    38941 gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg
    39001 cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata
    39061 aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac
    39121 cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta
    39181 gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg
    39241 agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt
    39301 gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg
    39361 tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc
    39421 ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa
    39481 tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat
    39541 tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg
    39601 attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta
    39661 gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa
    39721 caagggctaa aaaaaataaa acacgtttca ctttaaatca ctccatctgg atttgttagt
    39781 actatcttat cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca
    39841 agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca
    39901 taaaaaaatc aatgttaacg ctttctttat tagctatact cggtgtatca attgaaatgg
    39961 gggataaaaa atgaaaaaca aatttagaaa gattgttggg actatcgcta ttttgaccgc
    40021 aagcattcta ttaagtgcgt gtggcaattc aggatctgca gatgatagta cggggtatgt
    40081 aggaatcgct atgccgacca aatcagcaga aagatggatc gctgatggga acaacatggt
    40141 ctcggaatta gaaaaactgg gttataaaac agatcttcaa tatggagaag ataaagtaga
    40201 aaatcaagta gcacaaatag aaaatatgat tacaaaaggg gtagatacac ttgtgattgc
    40261 ttctatcgat ggttcggctt taactgatgt gttagctaaa gcaaaagaag cagatatcaa
    40321 ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt acgcgacttt
    40381 tgataatttt ggagtaggtg tatcgcaagc agcttatatt gaagagcact taggattgaa
    40441 agaaggaaaa ggaccattca cgattgaatt atttggtggc tcgcctgacg acaacaacgc
    40501 actaatcaat tacaacggag tgatgtctgt tttacaacca tatatggaca acggtcagtt
    40561 ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct acgcttcgct gggatggttc
    40621 tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat tatactgacc aaacattgga
    40681 tgctgttttg tcgccatatg atccaataag tttaggaatc atttcgtcat taaaaggcgt
    40741 ggggtatggg agtgaaagta aacctttacc tgtgattact ggacaagatg cgacagttgc
    40801 tggcgttaaa tcgatcattg ccggggaaca aacacaaacg atctttaaag acacacgtat
    40861 cttagcaaaa aatacgattg aaatgatcaa agcaatcagt gatggagaag aggttccggt
    40921 caatgatact gaaacttatg ataatggggt gaaaactgtc cctacgtatt tagcaaatac
    40981 agtatctgtc gataaagata attatcaagc agagctgatc gacaccgatt attataaaga
    41041 atccgatctg aaaaattaag aggaaccaaa caaggaacta aagagatcta agtcagagag
    41101 aggataagaa ttatggcgga ttgtatttta aaaatggcag atatcaccaa aaaattttct
    41161 ggaatccgag cgttagattg cgtgaatctg atgattgaac gtggggaaat ccatgcattg
    41221 tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca
    41281 tatggctcct atgacgggga aatcttttat gatggaaagc tgtgtcaatt taaagatttg
    41341 aaagatagtg aagaaaaagg catcgtcatc attcatcaag aattagcact aagtccttat
    41401 ctttcaataa aagagaatat ttttctcgga aatgaacaag cagaaagagg cgtgattgat
    41461 tgggaactga cagaaaagaa aacatatgaa ctgctgaata aagtcggttt gtctatcgac
    41521 ccgaatcttc tagtatccca gattggcgta gggcaacagc aactagttga aatagctaaa
    41581 gcattatcaa aatccgtccg tctgttgatt ttggatgagc ctaccgctgc attgaatgaa
    41641 gaagaaagcg cgaatttgct gagattgatc cgagagttga aagagcaagg tgtaacctcc
    41701 atcattattt cacataagct gaatgaaatc gtggatatcg cagatcgaat caccatttta
    41761 cgggatggac agacgattga gacactagaa aatgaaatga tcagcgaaga gcatattatt
    41821 cgcgggatgg tgggcagaga tttagttgat cgttatcctg aaagaaaacc ggatattgga
    41881 gaagtttatt ttaaagtaga agactggacg gtctatcacc ctcttgatca cgagcgaatc
    41941 ataaacgacc acatcaactt gcatttgaaa aaaggagaga ttgtaggaat agctggactg
    42001 atgggagcgg gcagaactga attcgctatg agtctatttg gacattctta tggaagcaag
    42061 atatccggac gtatattaaa ggaaggggca gagatatcag ttaagaccgt tccttcagcc
    42121 attgcaaacg gactcgctta tgtctcggag gaccgaaaat ctttaggact gaatctgctg
    42181 atggatattc gggaaaacgt gacgctgagc agtttggata aaataagtca aaaaggagta
    42241 ttagataaag aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa
    42301 acaaactcca tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt
    42361 ttatccaagt ggctgatgac acaacccgat attctttttt tggatgagcc aacccgtggg
    42421 atagatgttg gggcaaaata tgagatctac acgattatcg aagaaatggc agcaaatggc
    42481 aaagctgtct gtcttatctc ttctgaactt cctgaaatat tagggatgtg tgatcgaatc
    42541 tatacgatga acgaaggacg tattacagga gaagtctcgc gagaagatgc agatcaggaa
    42601 gtcttgatga aattaatgac gaaagaagag gtgggttaag ttggaacagg caaaaaatct
    42661 tacggataaa aaagagaaaa aactaaattt gaaaagtaga gcagctgatc tttttggtcg
    42721 ctacagcatg gtgatcattt tagcgggact acttattgcg tttcaattga tgacggatgg
    42781 tattttctgg cgtccattga atataactaa ccttgttctg caaaatagcc atatattagt
    42841 actagctgca gggatgttat tagttgtgct cttaggtcat gtggatttgt cggtagggtc
    42901 agtgatggcg tttgttggtg caattgcggg catgatgatg gtcaatcgcc aaatgaaccc
    42961 gtggatgaca gttatcgcat gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt
    43021 ttgggtagct tatgcaggga taccggcgtt tatcgtgact ttagcaggat tgctgatgtt
    43081 tcgtggactg acacaagttg ttctaggagg ccaatctcta gcaccttttc cagctgtttt
    43141 ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa ggaagcagcc ttcacttctt
    43201 gacattgatt ttaggaatga tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag
    43261 aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca acagctttct tggtaaaggc
    43321 ggtatttaca gcagtcgttg tactaggaat aacttatatc tttgcctctt atcaagggta
    43381 tccgattatt ttgatcattc ttggtttgat cgttgcagca tatgggtttt tgacaaataa
    43441 aacagtagca ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc
    43501 agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc aacatgggag tcatggcagc
    43561 tttagctgga ttagttttag ctgctcgatt gaatgcggca acacctcaag caggtacatc
    43621 gatggaactg gatgcaatgg cggcagtata ttttggtggt gcttcgactt ccggcggcat
    43681 tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga acaatggcat
    43741 gtcgatttta ggcgtcgggg tagactggca gcaggcaatc aaaggattga tcttgctttt
    43801 agccgttgtg ctagacttgt ataataagaa aaaacgaaca acgtaggaga acgccggtta
    43861 ttcagaaaaa agactgtgac aagacatttg tcacagtctt tttttcgcta aaagcgaaca
    43921 ttcattgttt cgtttgtata gactaaaaat aatatatgtt tttttaccat tcgtataaat
    43981 tctctttttg ctcttgattt ttttgcttaa ttgagttaaa ttacataggg cgctaaaaaa
    44041 agccttgatt aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg
    44101 taatgaaagt atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata
    44161 gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag
    44221 tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg
    44281 gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa
    44341 tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct
    44401 ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc
    44461 atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa
    44521 cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg
    44581 gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga
    44641 caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc
    44701 gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc
    44761 gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg
    44821 gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat
    44881 tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat
    44941 ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg
    45001 gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt
    45061 ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc
    45121 tgcattaagt aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg
    45181 aattattatc gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa
    45241 gatttttgtc acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc
    45301 ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga
    45361 gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa
    45421 cggaaggatc aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg
    45481 tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac
    45541 gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc
    45601 aagaatcgat tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca
    45661 aaaaataaga gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga
    45721 gatgccagat gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt
    45781 agcggaagaa aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg
    45841 gtatttggaa agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg
    45901 cggtcagcaa gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc
    45961 tgtagagtca ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt
    46021 atttgggata ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca
    46081 taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt
    46141 gatgagtcaa aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat
    46201 tattgaagac gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat
    46261 acgaacgatt gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa
    46321 aacaacgaaa atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc
    46381 gcaagaaatg atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt
    46441 taatgaagca attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg
    46501 actgataagc gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg
    46561 ttactatgcc tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt
    46621 agtagatatg ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat
    46681 cagaatcaat tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa
    46741 aattctagaa aagtggttaa cagaataatt tcacaaagta aatagattaa a
//
LOCUS       DAZUMB010000006        44502 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000006 DAZUMB010000000
VERSION     DAZUMB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.006"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACG80L_000265"
     CDS             complement(38..1189)
                     /locus_tag="ACG80L_000265"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS5022557.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACG80L_000266"
     CDS             complement(1202..1639)
                     /locus_tag="ACG80L_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5022558.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACG80L_000267"
     CDS             complement(1654..3420)
                     /locus_tag="ACG80L_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS5022559.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACG80L_000268"
     CDS             3684..4361
                     /locus_tag="ACG80L_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5022560.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACG80L_000269"
     CDS             4364..5491
                     /locus_tag="ACG80L_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022561.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACG80L_000270"
     CDS             complement(5691..6131)
                     /locus_tag="ACG80L_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5022562.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACG80L_000271"
     CDS             complement(6142..8175)
                     /locus_tag="ACG80L_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS5022563.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACG80L_000272"
     CDS             complement(8177..9880)
                     /locus_tag="ACG80L_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5022564.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG80L_000273"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG80L_000273"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS5022565.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACG80L_000274"
     CDS             complement(10963..11988)
                     /locus_tag="ACG80L_000274"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5022566.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACG80L_000275"
     CDS             complement(12197..13066)
                     /locus_tag="ACG80L_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5022567.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACG80L_000276"
     CDS             complement(13525..15417)
                     /locus_tag="ACG80L_000276"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022568.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACG80L_000277"
     CDS             complement(15414..17141)
                     /locus_tag="ACG80L_000277"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022569.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACG80L_000278"
     CDS             complement(17157..17807)
                     /locus_tag="ACG80L_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS5022570.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACG80L_000279"
     CDS             18011..18307
                     /locus_tag="ACG80L_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5022571.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACG80L_000280"
     CDS             18479..19099
                     /locus_tag="ACG80L_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5022572.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACG80L_000281"
     CDS             complement(19191..20357)
                     /locus_tag="ACG80L_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5022573.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACG80L_000282"
     CDS             complement(20369..20722)
                     /locus_tag="ACG80L_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5022574.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACG80L_000283"
     CDS             20850..21872
                     /locus_tag="ACG80L_000283"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS5022575.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACG80L_000284"
     CDS             21891..22052
                     /locus_tag="ACG80L_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022576.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACG80L_000285"
     CDS             complement(22311..22745)
                     /locus_tag="ACG80L_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022577.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACG80L_000286"
     CDS             complement(22855..24714)
                     /locus_tag="ACG80L_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5022578.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACG80L_000287"
     CDS             24715..24855
                     /locus_tag="ACG80L_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS5022579.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACG80L_000288"
     CDS             complement(24832..25992)
                     /locus_tag="ACG80L_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5022580.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG80L_000289"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG80L_000289"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS5022581.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACG80L_000290"
     CDS             complement(27739..28437)
                     /locus_tag="ACG80L_000290"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5022582.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACG80L_000291"
     CDS             complement(28394..30055)
                     /locus_tag="ACG80L_000291"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS5022583.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACG80L_000292"
     CDS             30273..31673
                     /locus_tag="ACG80L_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS5022584.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACG80L_000293"
     CDS             complement(31925..33004)
                     /locus_tag="ACG80L_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5022585.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACG80L_000294"
     CDS             complement(33090..34049)
                     /locus_tag="ACG80L_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5022586.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACG80L_000295"
     CDS             complement(34061..35572)
                     /locus_tag="ACG80L_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS5022587.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACG80L_000296"
     CDS             complement(35594..36157)
                     /locus_tag="ACG80L_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5022588.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACG80L_000297"
     CDS             complement(36163..37026)
                     /locus_tag="ACG80L_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5022589.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACG80L_000298"
     CDS             complement(37040..37936)
                     /locus_tag="ACG80L_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5022590.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACG80L_000299"
     CDS             complement(38111..39400)
                     /locus_tag="ACG80L_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5022591.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACG80L_000300"
     CDS             complement(39372..40889)
                     /locus_tag="ACG80L_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS5022592.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ACG80L_000301"
     CDS             41087..41977
                     /locus_tag="ACG80L_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS5022593.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACG80L_000302"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACG80L_000302"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS5022594.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ACG80L_000303"
     CDS             complement(42886..44358)
                     /locus_tag="ACG80L_000303"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS5022595.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12830 a         9316 c         7718 g        14638 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa ac
//
LOCUS       DAZUMB010000007        43423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000007 DAZUMB010000000
VERSION     DAZUMB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.007"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..825
                     /locus_tag="ACG80L_000304"
     CDS             145..825
                     /locus_tag="ACG80L_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5022596.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            915..1949
                     /locus_tag="ACG80L_000305"
     CDS             915..1949
                     /locus_tag="ACG80L_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5022597.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1963..2298
                     /locus_tag="ACG80L_000306"
     CDS             1963..2298
                     /locus_tag="ACG80L_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022598.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2282..4762
                     /locus_tag="ACG80L_000307"
     CDS             2282..4762
                     /locus_tag="ACG80L_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022599.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4818..6377
                     /locus_tag="ACG80L_000308"
     CDS             4818..6377
                     /locus_tag="ACG80L_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5022600.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6374..7738
                     /locus_tag="ACG80L_000309"
     CDS             6374..7738
                     /locus_tag="ACG80L_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5022601.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7909..8979
                     /locus_tag="ACG80L_000310"
     CDS             7909..8979
                     /locus_tag="ACG80L_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022602.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            8994..9662
                     /locus_tag="ACG80L_000311"
     CDS             8994..9662
                     /locus_tag="ACG80L_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022603.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9682..10260
                     /locus_tag="ACG80L_000312"
     CDS             9682..10260
                     /locus_tag="ACG80L_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5022604.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10663..11574
                     /locus_tag="ACG80L_000313"
     CDS             10663..11574
                     /locus_tag="ACG80L_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5022605.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11830..12747)
                     /gene="rnhC"
                     /locus_tag="ACG80L_000314"
     CDS             complement(11830..12747)
                     /gene="rnhC"
                     /locus_tag="ACG80L_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS5022606.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12949..13386
                     /gene="zapA"
                     /locus_tag="ACG80L_000315"
     CDS             12949..13386
                     /gene="zapA"
                     /locus_tag="ACG80L_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS5022607.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13444..13991
                     /locus_tag="ACG80L_000316"
                     /pseudo
     CDS             13444..13991
                     /locus_tag="ACG80L_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            14106..16466
                     /locus_tag="ACG80L_000317"
     CDS             14106..16466
                     /locus_tag="ACG80L_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5022608.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16553..16867
                     /gene="trxA"
                     /locus_tag="ACG80L_000318"
     CDS             16553..16867
                     /gene="trxA"
                     /locus_tag="ACG80L_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS5022609.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17049..18827
                     /gene="uvrC"
                     /locus_tag="ACG80L_000319"
     CDS             17049..18827
                     /gene="uvrC"
                     /locus_tag="ACG80L_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS5022610.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            18972..19871
                     /locus_tag="ACG80L_000320"
     CDS             18972..19871
                     /locus_tag="ACG80L_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022611.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19864..21126
                     /locus_tag="ACG80L_000321"
     CDS             19864..21126
                     /locus_tag="ACG80L_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022612.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21289..23187
                     /locus_tag="ACG80L_000322"
     CDS             21289..23187
                     /locus_tag="ACG80L_000322"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS5022613.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(23486..24205)
                     /locus_tag="ACG80L_000323"
     CDS             complement(23486..24205)
                     /locus_tag="ACG80L_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS5022614.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    24395..24442
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24542..24874
                     /locus_tag="ACG80L_000324"
     CDS             24542..24874
                     /locus_tag="ACG80L_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022615.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24861..26864
                     /locus_tag="ACG80L_000325"
     CDS             24861..26864
                     /locus_tag="ACG80L_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS5022616.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26883..27353
                     /locus_tag="ACG80L_000326"
     CDS             26883..27353
                     /locus_tag="ACG80L_000326"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS5022617.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27374..27958
                     /locus_tag="ACG80L_000327"
     CDS             27374..27958
                     /locus_tag="ACG80L_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFS5022618.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            27973..28959
                     /locus_tag="ACG80L_000328"
     CDS             27973..28959
                     /locus_tag="ACG80L_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS5022619.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            28949..29260
                     /locus_tag="ACG80L_000329"
     CDS             28949..29260
                     /locus_tag="ACG80L_000329"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS5022620.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29282..31063
                     /locus_tag="ACG80L_000330"
     CDS             29282..31063
                     /locus_tag="ACG80L_000330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS5022621.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31056..32432
                     /locus_tag="ACG80L_000331"
     CDS             31056..32432
                     /locus_tag="ACG80L_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS5022622.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32439..33074
                     /locus_tag="ACG80L_000332"
     CDS             32439..33074
                     /locus_tag="ACG80L_000332"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS5022623.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33104..34459
                     /locus_tag="ACG80L_000333"
     CDS             33104..34459
                     /locus_tag="ACG80L_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5022624.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34651..36045)
                     /locus_tag="ACG80L_000334"
     CDS             complement(34651..36045)
                     /locus_tag="ACG80L_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5022625.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36137..37108
                     /locus_tag="ACG80L_000335"
     CDS             36137..37108
                     /locus_tag="ACG80L_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS5022626.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37105..37887
                     /locus_tag="ACG80L_000336"
     CDS             37105..37887
                     /locus_tag="ACG80L_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS5022627.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38056..38427)
                     /locus_tag="ACG80L_000337"
     CDS             complement(38056..38427)
                     /locus_tag="ACG80L_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5022628.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38561..39004
                     /locus_tag="ACG80L_000338"
     CDS             38561..39004
                     /locus_tag="ACG80L_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5022629.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39047..39856)
                     /gene="yidA"
                     /locus_tag="ACG80L_000339"
     CDS             complement(39047..39856)
                     /gene="yidA"
                     /locus_tag="ACG80L_000339"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS5022630.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(39875..41242)
                     /locus_tag="ACG80L_000340"
     CDS             complement(39875..41242)
                     /locus_tag="ACG80L_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5022631.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41226..42056)
                     /locus_tag="ACG80L_000341"
     CDS             complement(41226..42056)
                     /locus_tag="ACG80L_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS5022632.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42219..42656
                     /locus_tag="ACG80L_000342"
     CDS             42219..42656
                     /locus_tag="ACG80L_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS5022633.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42732..43352
                     /gene="rpoE"
                     /locus_tag="ACG80L_000343"
     CDS             42732..43352
                     /gene="rpoE"
                     /locus_tag="ACG80L_000343"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS5022634.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14220 a         7472 c         9221 g        12510 t
ORIGIN      
        1 gaatattcat gtaaacgatt ttattgtttt tttctctttt atacaaaaag ggtcataatt
       61 tcaagtaagc ggatgaattc atgaggattt tttgcatact gtctctacta tacttaggtc
      121 actaagaaaa aggagtgatt tattatgaaa aagaacatga ttgggacatt attactttca
      181 acacttcttc taggcggctt ggcaacacca gcttttgctg agggtcaagc gacttcaaaa
      241 ggagatatta cctttactga accaacaaat accgttgagc ctcttaaccc tactgatcct
      301 tcaaaaccag tagagccagc agatccggaa aatccagcaa ctggccagac aggttcttta
      361 acattggatg tagttcctga attgccattt ggtacacacg agattgaaag cggaacaaaa
      421 acgtatcaag tagacgcttc aaaaaacgat acaccttatc tccaagtatc agatcgtcga
      481 ggtgtaggtg cagatggcca agcacaaggg tggaacgtga cagtttcggt ttcagatttt
      541 gtaaacggtt cacaagtttt gcaaggcgct gaattagatt ttggaacatc cactgttaaa
      601 agtacgtcag ataacgaatc cactggacca actagtcaaa ctgtaactgg gttgagtaaa
      661 gcaagtgcag cgacaccaat atttacagca gcaaaagatc aaggtctggg aacatggctt
      721 tctgtttatg atccagcaaa tatcacactg aaagtgccga aagcagcagc tggaacattc
      781 acggctgatt tgacttggaa cctcgtggct ggcccagtag catagagtat gaaataaatt
      841 caacaaactg cggtgaaaat actataggtg aaggattttt taccgcagtt gttgttgaaa
      901 ggatgtggtg gacaatgaat caaagtaaac gactgtttgt ttcgtttttt tctattttaa
      961 gtctattttt catttttccg tcaatcagcc aagcaaagga ttcagcagga gactttggaa
     1021 tcaaaccggt atttccagag aaccaaatag ataaagcaat cggttatttt gatttattag
     1081 tggcaccgga acaaaatcag acactagaag taattatcag caattcttca gatgaagaac
     1141 gaacatttga ggtctctgtc aatccggcgg taacgagtga tggtggaacg atcgattata
     1201 gtcaaaaaaa cccaacgtta gatgagacat tgccgtttga tgtccgtgac gttttgctca
     1261 ttgcgaaaaa ggaaatcaat gtatcagcac atgcagaaac aactatacct atagaagtga
     1321 aaatcccagc aaaaagcttt aagggacgtg tattagcggg tattcatgtt tcacctaaag
     1381 aagaagctga aacagaaaat gctacagaag gagcacaaat caaaaatcgt attgcttata
     1441 atctcgcagt cgttttacaa gaaagtcaag agacgataga accggatctg aaacttttat
     1501 caggagattt ggacgaggtc aacgcaaagc caactgttca gcttcgtttc caaaatcctc
     1561 agcctaggat tatcagcaat ttgatattta cgagcaagat cttttatgaa aatcagcttt
     1621 atattgaaaa tacgtctaat gaatttttag ttgcaccaaa ttcgaatttt cacttgaatc
     1681 ttgatttagc tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat
     1741 caggcgacag taatgaatgg cgatttacac aaaactttac gatcaaaaaa gaaaaagcac
     1801 aaaaagtaaa tgaaaattcc gtttttgcag tacaagaaca atctttccca tggctttatg
     1861 caggattggt tgctgcagtt ctagtgttat ggttgatttt gctgtttttg tgggtccaca
     1921 agaaaaagaa aaaaggagaa acaacatgat gaggtgattt cgttgaaaac atcgatctta
     1981 ttgggaggga ttattgcaag tttgctgttc ttcagctggc aagagccagt ccaagcagat
     2041 accaaaagtt caggtacgat tacattttat gatctaagtc cagttccgca tccaagtggt
     2101 tcagtcaatg gcgcatcaaa acagcagaca cagctaccga tttataaaga atctaatcaa
     2161 catggcgtat tcccggagac gaatgaagaa aagatccgtt ttctttattt gatcattggt
     2221 atagaattat ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa
     2281 catgttgcta caaaataaat tttcattttt tcgctccttc ttttttactt cgctttttgt
     2341 tttaagtata tttatctttt caccttcggc agaagctgtt ggagcaacgc ctcctgccat
     2401 ttcttatgat ccatacacgt atgaaaatat cgtacctcca aatggtggac gtagtgcagt
     2461 caatggcggg aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat tgactgctac
     2521 ttatttgaac gatggttcaa atgatccttg gaaaggtggc acggtccgtt ttcattggga
     2581 aagccatgtg aaactaacaa atgtacttga tttggtgaat atcactgcac aaggatttgc
     2641 atttgtggcg atcatcaatt tgccggaaca tgtatcaagc agtgatgtat taaaagcaat
     2701 tgactggaac aacgcctact tgtatattgc cggtgccccc atcaaggtta ctagcggtag
     2761 tttattatca gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg atacaaatac
     2821 tctattggca gatattattg ctattattac gcgaggcgaa ttcaacgtgg acgatatccc
     2881 gcttcaattt gatgttacag ctaatgtcgc gaagatgaca gaaaacggag ataaatatga
     2941 tacgagtcca aacaaaattt tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc
     3001 cgttttttct gttgattttt atgattcgaa taatattatc caagatcgta tgttcaattc
     3061 gattttagct ccagctgcag gtagatttta tcctaaatta actggcggaa acttgaatcc
     3121 aggagcatat gcattttctg caagtgcaga gataaatacg tggaatagtt acatctcaac
     3181 aagtgattca acaggaaaat ataatacatt tgctgatacc acagaatacg tggatggaaa
     3241 taaccagaac cttccaggct ctaccaatag tcggacattg acgatggggc tgactgaagg
     3301 acgttttgat cagttgccat ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa
     3361 aaacgttaca caaggagcaa tactgactca tactccagca agcacaagtg gattgaatca
     3421 gacagccaat atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc
     3481 tttaaaggta ttggagcagt acaagctaga cggaacggtt ttaccaacaa acttaacagc
     3541 cgatagtcag tggggaaagc tcctttcttc tgcatcgtac caagtcacgg ccaattggtc
     3601 agcaactgaa ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag gaacccaaaa
     3661 attagcgaca ggatggacag atcaactttt ccaaacgatc caatcaaata acgggggtgt
     3721 aaatacagct acttctacac tacctgcatt ggcttcaggg caatattatt ttgattatcg
     3781 cttggtggac gatgtactta ctaacatgta tccgtctttt gcgtacaaat ggcagtctga
     3841 acaagctgga atcaccactc ttcctcaaat aacagtggct aattttccat ctgtgagtgc
     3901 agatagtcag ctaaaaaacc ttagcagaca acctgagaca ggagatcccc tgtctgctct
     3961 atcaggagat acgattcaag aaaatgtgac attcacgtta acaaaatctg gggatgcgat
     4021 aaccgacaaa aaaatcacga tctcacttcc tgaaaatact acttatatca atggttcctt
     4081 aaaattaaat ggtacaacta tcgcagatac cggcattcaa caaggagttt ctgtcccagc
     4141 agatctattg agcaaaatag gagataccat tcatcttact tacaactatc aattaaatac
     4201 tgtagatact tctgtacaat ccgtcagcat actgacaaag gcagctgtac tttccagcaa
     4261 tattacatta gcagatggtg ctaagcttcc taacccagtt gtccaaacat ctgctaaaac
     4321 aattcttgtc cccaaacaag aattaacatt agttaatgta ccagatgatt ttacatttgg
     4381 taacgatctc cccaaaccgt tgaagacaag ttattatgaa gcaaaaggcg atttttcttt
     4441 tgatgtgcgg gatactcggc ttccaagcac gtctccttgg caactgacag ggacattgac
     4501 ttctttattt aagaatgatc aaggtcaaga attaagcgga acaaaattat acttcaatca
     4561 ttctggatca aaacaattga ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg
     4621 aacagctaaa ggagaggttc tcgttgactt tcccgatacc gatggtttac tcttagaggt
     4681 aaatagcagt acgaatgctc aacctggcgc aacgtatcaa ggcatggtca catgggaact
     4741 gacagctggg ccaaccagct aagtgattag aatttgtatg gatctgtgat aactgtcaca
     4801 gatcctcttt ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa atgcttggag
     4861 gagtggactt ggaacttttc agtttgcttg aaaaaaatga acaacggcaa atcaaacttt
     4921 tccaactttt attagaggaa aaaggagaga tttccgttaa taacgtactt acccgcttat
     4981 cagttgatcg ggctaccttg aaagaagatc tgttttattt acaagctaac ttggaaaata
     5041 tcaaacaatt ggaaattgtt atccaagaag ggaatatttt attaatcaga catggaaata
     5101 tagcagcagt cgatgtttat tatcattgct acttgcgcta ttctacaaaa tatcagatac
     5161 ttctttatct gttggaaaat ggctcatttg atcggcagaa acttgtcatg gaacttaata
     5221 tcagttcggc tactttgtct cgcagagtga aggaattaaa tgattcttta aatgagtttc
     5281 atctgaaaat aaaaagcggg cggctgaccg gtccagaaag tcaaatttgt tatttttatt
     5341 tccaattatt ttggtttgga cgtccttatc ttgaaaacaa aaaagaatac agtgatttat
     5401 caggaaacac ttttttagat ctgctgcaaa aagagtttag ttttactatt acagaagaag
     5461 gacgattgaa atttgaaata tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa
     5521 aatccaaacc agattatagc aatcttcctg ttggattgtt tcaaacgaca caatttttat
     5581 tggacctcca tacatttttg acccgttact tctttcaata tgcctttcct tggaatcaaa
     5641 atgaaacgtt ggttttttat cttttcatga tcagtaactt tacattagat acacataact
     5701 cgcatgtcca ggagtttttg ataagcgaaa aatacaaaga cgacgtagta gctcaattga
     5761 atcgaacgtt taaacaatcg atagaagaat atttctcttt ttatgttttt ccagaagaga
     5821 tttcgcaaaa gattgcgttg acagtgatgc aaaatcacgc tcattttgcc tattttagag
     5881 gatggatgaa tatttttggc caagaaggat taaaacagcg gttaactgaa gtagaagata
     5941 aacaattcct ggttttatgt gaaagattgg tcagacaaac atttgctatt gttgatttta
     6001 ctgaagtaga cgaggaaaat gcccgctttg aattatttgc tcgctatgca tctattttgc
     6061 ggacgctgat caagcagtta gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa
     6121 ggctaatggc ggatatcgtg aaagaaatca tccaggaaca attgctgaat acgatcaaat
     6181 atgatttagt cgaatacgag aaagaaaagc agtatgatat cattttgact agccaattga
     6241 aagagtaccc gtcacagaag tccgctaaag tttttgtatt cactagcaac gaatataatt
     6301 atgatttccc ctatttgaac caatttttaa aagaatgtta tttagaaaaa ttgaatttaa
     6361 ggatgaataa aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca tcacagctgc
     6421 cctaaatcta agaaaacttc aaccgaaagc agaagtttta ctgatcgatg aagatcataa
     6481 agatggacta ggctatgttt caaatggaat caacctttat ttaaaaggaa agatacgtag
     6541 tttagctgaa gttgcccata acatgcggac tcttcaagaa agtggggcaa aactgatcac
     6601 cgaatggaga gtaactgaac ttgatccaga caaacatcag ctcatcctcg catccaaaga
     6661 aggaaaaaac gagaccataa cctatgataa attaatcgta gcaacaggtt cctctcctgt
     6721 caccttgtat aaacaaattg aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc
     6781 taagcaagta cttgccgctt taaaagaagc aaaagaagta gtgatcttcg gtgctggtta
     6841 tattggattg gaattagcag atgctttgag aaataaagga tacattattc atctagttga
     6901 ttatatgccg aatgttttat caagatattt cgataaggat atgatcaaca gttttcaaaa
     6961 tcagctgcaa actaagcaaa tcaattttta tccaaatgaa ttcttgatcg attggaaaaa
     7021 atcagaagag aaagtagtat ccgtacaact tctatctcaa gcaataaagg cagatatggt
     7081 cattttttct gcccaaactc gtcctaatac gacattgtta aaagaaaaag tggctttgta
     7141 tgaggatgag actgtgatag tcaatgaata tctgcagaca agtgatccgg acatctatgc
     7201 gattggagat atcgtcccag tcagttttga caaaaataaa cggcatctct ttttaccttt
     7261 agttactcga gctgtacata tggcaagagc agtagcgcta actttgagtg gtcagccaac
     7321 cgcttacgac ctcagacaaa agataacagc agcagtcatt atagactact ttttaggaac
     7381 tgtagggctg acagaagatg aagcgccatt cttggaacaa aataccggtt cgtgttcagg
     7441 agaatttgat ctatttccac aatacgatga agaaaacaaa acagtaaatg caaagctgat
     7501 ctaccatccg gatacattag aaataattgg cggacagctg ataagccaag aattcttatt
     7561 atcagatttg aacctgcttg cggatatcgt gaaacacaaa actacgattc cacagctagc
     7621 agttgaggat tttggatttc tgtctgaaca cacctctcgt tttcactacc tcaatgagtt
     7681 agcttttaaa gtactagcta agagagcaaa tagcaataga gtagataaaa gactgtgaga
     7741 aatatcacgg tcttttattg tgtggggata taataaatat taatcaatcg agaataaaaa
     7801 attcatataa tgaaatagcg gaagaatttt ttgagcataa tattcgccaa aaatttatca
     7861 agtgataaaa ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca
     7921 ttaaatgaaa ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg
     7981 atcgcctacc tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga
     8041 acagcagtcg acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat
     8101 ctagggatgt ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg
     8161 tatcttgccc agacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc
     8221 aatgttagct tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga
     8281 aaagcttttg ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt
     8341 ctagccgtag gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc
     8401 tttacggatc atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac
     8461 caagagatcc gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt
     8521 gtcagaggta aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc
     8581 atcaatgaat tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt
     8641 ttgatcgtta ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag
     8701 attaatatct ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct
     8761 gtagtggcac aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca
     8821 gtgggaacat cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt
     8881 ggcgtcatta gcttgttgat gctggtagta gctgtgttag gcgcactatt ttctgttcgg
     8941 acaatcgtaa aaatcgatcc gttgaaggca attggataag aaggaggaag agcatgaaag
     9001 caatagaatt taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat
     9061 tgaaagaaac aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg
     9121 gttcaggtaa aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct
     9181 ctgtatttat caatgatcaa gaattcagta atcaaaaaga aagtgaacgc gttaaactac
     9241 gttttaaaga aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa
     9301 aaaagcagtt attattagta gataagatca aacatgaaca tcgtcaaaaa gaagcaaaag
     9361 aattatttga gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg
     9421 gtggagaacg gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc
     9481 tagcagatga accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct
     9541 tggcaaaaga atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc
     9601 tgattgacaa atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat
     9661 aaccaaaaag caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt
     9721 ttgaaagaag cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagag
     9781 atcgcacaaa aagttgggat cacccaaccc aatctctatc attatttcgg tgataaagaa
     9841 aaactatata cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa
     9901 atcgttcaaa caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg
     9961 attgagacac atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca
    10021 aaggaaacaa gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa
    10081 gaaatctttt cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca
    10141 aggcattttt tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa
    10201 ccgctaaatg tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa
    10261 agctgttata tagggagaaa aatagttagg tgaagaagca agatatgacg aagatcgtta
    10321 tatcttgttt tttttatata ggtagttttt cataaataca aatttgttat tctctcatga
    10381 ttatcagggt atatttttat ttattatgtc ggtgttccag gaagtgtttt aatttaataa
    10441 cgaagaaatt aattgtttgt agaatgaagg aaactattta ttgggtagat gaatcaccac
    10501 gaatagtcaa aaggagattt caggatatgc caaaaatgaa aaaaatcatt ttagcagtaa
    10561 tgttattttc tacggttata tcttttacag cccctttttt gcgtacttaa gctgatttta
    10621 agttttgacc cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa aataattgga
    10681 gcttttgtat ttgcaatcat tatcattatt atctgttttc aaatagatgt ttttggtcaa
    10741 aaaaagatag ttggacaaac ggtcagcagt acgaacagta cggttacaaa gacaaaggaa
    10801 tcatttgatg atacagaaca agagacatta atcaaaatcg gaaaaaatga ccgtacacta
    10861 tatacagatg actcgctaac taccccactt gcaactataa acggcggaga gctggcggag
    10921 tttctttctg aaacaaaaaa tgcttatcag atcaagacaa atgatggcta tactggatat
    10981 ttagccctcg aagatggaac caaagtcaca aagaatatac aaacaaaacc ggaagagcta
    11041 tctgatgcag tgattgtatt agatcctggg catggcggaa atgatactgg ggcattaagc
    11101 aatgatgaac aaacagaaga aaaagatata acattaagta cagccataaa agtaaaaaag
    11161 gcgctagagg atgctggagc aacggtttat ttgacccata ctacagatga attagtacag
    11221 cttggagata tatgcgacta cagtgaagaa aaaaaggcag atgtctttat cagtttacac
    11281 gcagactcta cggaatatgc gaatgaagca actggaatca cgacatatta ttactatgga
    11341 caagaggaaa cattagcaca gacaattgct gactccttca ctgatctgcc tctggattca
    11401 agaggaatct ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc ttctatttta
    11461 atcgagatgg gttatatgaa taatgatagt gatttaaagg aattagtaac agacagttat
    11521 cagcagcaga ttgctgcaag tttgacacaa gcattgacca cttattttca gtaataaata
    11581 agcccagcca aacaaaaata gctcctcata tttttcgttt ggccgggctt attatcgcag
    11641 atgcaagctt ttgaacaccg tttaaggaag aggttgtgag cctgccgttt aactccgcgt
    11701 cacaaggagc aatttcaaaa aacagcttct catgtttgat gactattgtg acttgtgacc
    11761 gaggagttgg catgagaaca ccgtttattc ggaacaaggg actgtgacat gacttttgtc
    11821 acagttccat tattttttta gcagcttcaa agctttttct gtattggcaa aatgaagttt
    11881 ggcatatttt tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc
    11941 agatttcgtt ccagctcctg atggaagggt gattccagct tcagctgatg ctttgtgtaa
    12001 ctcttccaaa aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg
    12061 ttctccttta gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata
    12121 ttttttaaaa tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg
    12181 ctcgattttt tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac
    12241 ggcattgtat tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata
    12301 ggggatggtt ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt
    12361 tgaatctcta acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc
    12421 acatactgtt agcggaccaa aatagctgcc atttccgacc tcatcactac ctaaaacgga
    12481 taattgactg atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc
    12541 ctgtgcctcc ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt
    12601 tccggatgta taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg
    12661 aatctgttta tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt
    12721 tgttgctttg ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata
    12781 ccgcaactca cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt
    12841 tatcatataa tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt
    12901 catggtacac taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa
    12961 aaaacacgct acaaagcagt gattgccaac caaacatata ctattatcgg acgggaaacc
    13021 aagcatcata tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa
    13081 ttatcaccac aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg
    13141 gatcaattga agaaacaaga aagaatcttg gagctggaag aagaaacagc agaattaaag
    13201 aaaaaaatga tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag
    13261 aacgaagctc gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat
    13321 gtggaagcac aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct
    13381 aattaagatc cttttatcaa aataagaaaa atttaaaaaa gttagaagag aaaggaacgt
    13441 agcatgctga gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt
    13501 cgcgggtttg ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc
    13561 cagcattttt acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc
    13621 accccaacat caaagttagc attttttgat caggtatttg cttttcacct agatgaagct
    13681 ttttatgcag gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt
    13741 gtgggggttt ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt
    13801 gcaggaggca tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta
    13861 cttgcctttg ttccattagt gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt
    13921 ttatcgttga gagaacgcca tttcttacca acaagatgta tgagctttgg gtaacgaata
    13981 ttttgaatta aggaaaatag tgctggaaaa tatttcgttt tttgtacaga gtagtttatt
    14041 taagaaggct gcgtagcgta tgtccggcac agctcttttt tcatagtgaa agataaggtg
    14101 ataaaatgaa taaccgaatc cttgaaacat tggaatttga aaaagtcaaa cagatggtca
    14161 gacaatttgt agtgactgca caaggaaaag aagaactggc agaattggta ccagtgagtg
    14221 agaaacaaac gatcacaaac tggcttcaag aaactgagga tggattgaaa gtgcaacgtc
    14281 tgcgaggcgg gatccctata cctaaacttg aaaatatccg tccccatatg aaacggatcg
    14341 agatcggagc agacttgaat ggagtggagc ttgcacaagt ttccagagta ttgtctacga
    14401 caagcgaatt gaatcgtttc attgatgatc tttctgacag tgaaatcgaa tttgcacgtt
    14461 tgtatatgtg ggcagatcag ttagtcacga tccctgtttt gagccgccgt ttaaaggaag
    14521 caatcgatga agacggacgc gtgactgatg acgcttctcc tgagttaaag agcataagac
    14581 agaacattcg gcgaagtgaa caagctgttc gtgaacagtt agatgggatc gtccgtggga
    14641 aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc
    14701 cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag
    14761 gacagacttt atttattgaa ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat
    14821 accaaatcgc tgaacgaaat gagattcaac gtattttaag tgaactttct gctgaacttg
    14881 ttccccatcg tcaagaaatc atccacaatg cttatgtgat tggtaaaatg gatctgatga
    14941 atgcaaaagc acgatttggt aaagaagtaa aagccatcgt gccgggaatc agtgaagaca
    15001 atcatgtcgt cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa gtagtttcta
    15061 atgacatcac aatcggaaaa gactatcaag ccatcgtcat tacaggacct aacacaggtg
    15121 ggaaaacgat cacgttgaag actttaggtc tgttacaact gatggggcaa gcaggattac
    15181 cgattcctgc aggtgaagaa agtcaaatcg gtatttttga ggaagtattt gcggatatag
    15241 gagacgaaca gtcgattgag caaagtctat cgaccttttc ttctcatatg acgaacactg
    15301 tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag
    15361 gaacagatcc gcaagaaggt gctgctctag caatcgcaat cttagatgat cttggtaaaa
    15421 agtctgctta tgtgatggca acaacgcatt atcctgaatt gaaagtttat ggatacaatc
    15481 gggcaaatac gattaatgcc agcatggagt tcgatgtgga tacactaagt ccaacgtatc
    15541 gtttgctgat tggcgttcct ggacgaagca atgcgtttga aatctctagc cgattaggtc
    15601 ttgatacaga agttattgat gaagcgaagc agttgatgaa tgatgagagc caagatttaa
    15661 acgaaatgat cacagattta gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa
    15721 tgcgtcattt tgtttctgag gcacaagaac ttcacgatga tttgaaagaa gcttacagct
    15781 atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca aacgaagtcg
    15841 tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc
    15901 agattgggca aggaaatgtg aaagagcatc agttgatcga tgcaaagacc caattagcaa
    15961 atctacatca agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa
    16021 aaacgttgaa accgggagac gaagtgttgg ttacgactta cggccaaaga gggacgcttt
    16081 tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat attaaaaatg aatgtgtccg
    16141 aagacgagct gacgcctgtt gctcctcaaa aagagccgac acaacgagtt atccatgctg
    16201 tacgatctga atctagtagt cacgtgccga atcaattgga ccttcgagga aaacgatacg
    16261 aagaagcgct aagtgaggta gatcaatatt tggattctgc cattctagct ggttatcctc
    16321 aagtgactat tgtccacgga aaaggaacag gcgcacttcg caagggaatt actgattatc
    16381 ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg
    16441 gtgcgacaat tgttaaattt aagtaaagct tactaaaggt aagcagatgg ttcgctatcc
    16501 aagtatagaa atgctatact aatcatagat tttaaaaatg gaggtctttg atatgtcaca
    16561 agtaattaca gataagacat ttaatgaaga aacagataaa ggacttgtat tgattgattt
    16621 ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg attttggatc agttggaaca
    16681 agaatatgat gaagaagaat tcagaattgc taaaatggat gtggatgaaa atccagaaac
    16741 tcctcaacaa ttcggtatca tgagcattcc aacattaatg ttgaaaaaag atggtcaagt
    16801 agtagaaaaa gctgttggtg ttcattcaaa agaacaacta cgtcaaatga tcgatcaata
    16861 tttataaaaa cttacagcaa aaataagctg agggtgggac tgaatccagt tccgtcctcg
    16921 gtttttttgc ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg
    16981 tcaggctgga ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg
    17041 agtaccagat gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct
    17101 gttacttgat gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa
    17161 aaaatcgtgt ccgctcatat tttactggca gccataatac gaaaacggaa cggcttgtta
    17221 gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag
    17281 agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa
    17341 cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaaag
    17401 tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga
    17461 caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg
    17521 agccttgtct ttactatcat ttgggacaat gtctgtgtcc ttattatttc gacgtagatc
    17581 cgaaagtcta tacgaaaatc gttgatgaag tgaaaaagtt tttaaacgga ggacacgaaa
    17641 aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa
    17701 aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa
    17761 tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgac aaaggatgga
    17821 tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt
    17881 ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg
    17941 aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg
    18001 tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag
    18061 ttgaattggc gaataagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca
    18121 gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga
    18181 ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg
    18241 cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa
    18301 aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt
    18361 cacgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag
    18421 gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg
    18481 ctggcatggc aaaaaacgat aaacataaaa caaatgagtt gctattcggt atagatttgg
    18541 ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg
    18601 aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt
    18661 cccgattaga tgaaattgaa aggttaggac caaaacgaaa aaaaatgctg cttaaagagt
    18721 tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc
    18781 cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt
    18841 tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg
    18901 ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa
    18961 ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg
    19021 tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg
    19081 gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag
    19141 aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc
    19201 cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag
    19261 aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgatttttgg atggaaaaat
    19321 ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa
    19381 aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat
    19441 tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa
    19501 aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg
    19561 atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc
    19621 gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag
    19681 aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct
    19741 tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc
    19801 ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa
    19861 tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt
    19921 tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga
    19981 aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa
    20041 atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct
    20101 gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca
    20161 actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca
    20221 caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc aagtcaatta
    20281 ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa
    20341 gtcaattttg attcaaaagg aaaaatgaca gttggagaag ataactcgac agtccaatat
    20401 gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc
    20461 attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt
    20521 acgactgcac aaaagcattt ttacggaaaa ctaacaggtg ttttacttgt tgcattaacg
    20581 caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa
    20641 atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc attcttgtgg
    20701 tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt
    20761 ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta
    20821 tctctaggcg gatatatgct tggattgata ctaggagctt cagatccaaa caatatcgtt
    20881 attcggatca cttcgtatat tccattctta tcttcatata ttatgccggt tcgcttagca
    20941 aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca
    21001 ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt gtatagcgaa
    21061 ggcggattat ggagttcatt gaaacaatcg atttcaatta tgagaaatga acgaaaaaaa
    21121 gcataaaata tagaaaaata tagaaggaag ctggacattt attgttccca gcttcttttt
    21181 tgtatagacc tatttctatt tttttattat attggatata tatattcatt gaaaatagag
    21241 cataacaact ttataatgtt gagtatacca atttttggag gcgttttaat ggaccgctat
    21301 ttagatttac tttcagagaa gttccctaca acggaagatg tcatcacgga aatcatcaat
    21361 ctcgaagcaa ttttgcagct tccaaaagga acagagttat ttttgagcga tatacatggc
    21421 gaatttcctg cttttgatca tattctgcgt atcggttcag gaaatcttaa agaaaagatc
    21481 aaagaccttt tctctgaaca aatgacggaa acggagatca gccaattcac tatttttacg
    21541 gcttatccag aatatgtgtt gacgactgat tggtacaaaa aacaagataa atcccagctg
    21601 atccatcgtt tgatcgatct tttacggttt acgacagtca aatacactcg atcaaaagtt
    21661 cgaaaaggat tgccgaaaga atatagctat atcattgagg aactcttgta tatcgatgat
    21721 cgtatcaacg ggaaaaaaga ctatgtaaaa aaaatcattg aacaacttgt cttgatgaag
    21781 gaagaagaaa aatttttaac aaaacttgcg cagacgatcc aaaaatctgt catcgatcat
    21841 ttgcatatcg tgggtgatat ttttgatcga ggaactcaag cagccaaagt aatggatcga
    21901 atcatggatt tttcctctgt agatatccag tgggggaatc atgatatttt gtggattggt
    21961 gcgtattgcg gctctgaggc atgtttattg acgcttttac ggattgccgc acgctacaac
    22021 tatttatttg aattagagaa agaatatgga ttaaatttac gtccattatt ttcttttgcc
    22081 catcaaacct atcagaagaa tccagtattt acccctaaaa acaggaaaga tctatctgaa
    22141 agagagcaag aagaattaga aaagatccat caagcacttt ctatcctcca attcaaatca
    22201 gaacatcagt tgttgaatcg acgacctgaa tttaaaatgg acgatcgtaa attgttggaa
    22261 aaaatcaact atgaggaatc aacgatcgtt ttagaaggtc agctctatga gttgaaaggg
    22321 acttgttttc aaacgatcca gccagatgat cctaaaaaat tcttggaaga ggaacaaaaa
    22381 gtgatcgata gtttgatgta ttcattccag cactcgttaa gaatgcaaaa acacatgacg
    22441 tttttgatcg aaaaaggggg aatgtatctc acgaacaatc agcatgtact gttccatggc
    22501 tgtatacctg tagatgaaaa ggggcagctc ttagcatttg aattagacca atcttatcgc
    22561 ggaaaagaac tgctgggatt ttttgaaaaa cagataactg aaagcgcaaa agatttgact
    22621 gcaaatgaag atttggcgac cgatttgatt tggtatgcgt ggagcgggcc attttcgcct
    22681 ttattcggca agagtaaaat ggcaactttt gagcgctatt ttctcacaga gcctcatacg
    22741 cacgtcgaaa aaagcaatcc ctattatcac ttgcgagatg aagagtggtt cagtgaaaaa
    22801 attttggcag aattcgatgc aaaagaagaa ggttcagcaa tcatcaatgg acacacgcca
    22861 gtaaaagtaa aaaaaggaga gtcaccgatc aaagcaaacg gaaaagcatt tgttattgac
    22921 ggaggtctat ccaaagctta tcaaaaaaca accgggattg ctggctactc attactatgc
    22981 aattcttacg gtttccaaat cgtgacacat tatccgttct tgcctattga acaacaattt
    23041 gcaaaaaaat ctgatcaaac gaatgtaaaa aaagtgatcg agcagcagct cccacgcaaa
    23101 cttaatcgga atacgacaaa aggaatggaa ttacagcaac agattactca actccaaagg
    23161 ctgttggatt atcgaaagga cgattgagaa aaatgctagc caacattgct caatagacta
    23221 aaaagcgact gcaacaaaag gcgtgtcgca gtctccttcg gaattagctc aaaaaacggt
    23281 aaaatattag aagctatttt tctgtttatt tctctaatac tcaggtcagg gcgctgattt
    23341 cacgacctct tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc
    23401 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt tgaacggcag
    23461 gctcacgacc taatcagaaa aacgattaaa attcattcac aaagaaatcg aataaacgct
    23521 gttcagatga atctacacga tgagaaagtt ccggatgcca ttggacacca aggattttgt
    23581 gcatattatc tttactttgg actgcttcaa tgagtccatc gttggaaaag gcgatggctt
    23641 ttaacaaagg agaaagatct ttgactgcct gatgatgata ggaattcaca aagtatgaat
    23701 cagggagaag gtcacttaat aaactgtccg aaacgatttt gacttcgtga gttgcaaact
    23761 gcggggcagt gggctgttgt tcgtgtttta cggaccattt tggatataat gaaagatctt
    23821 gataaagtgt tccctccaac acgacattca atagttgaag tcctcggcag actgcaaaaa
    23881 tcggttttct ttgtttcaat gcttccaaga tcaatgcctg ttcgaattgg tctcgttgaa
    23941 tatttgtttc cgtcagtttt ggatgagggt cttccatgta aagttgcgga ctaacatcct
    24001 gaccgcctgc aagaatgagt ttgtcgattt tctcaattag ttgaggcgct gaatctggag
    24061 acatgactgg cagaataaga ggtaatcctt gagcatctaa aactgcatcc acaaatccct
    24121 gcggtgtata gcttacttga tttccttgga aagtatctgt cgctcgaatc agttgattcc
    24181 cggcaatgcc gatgattggt ttcattttta ttccccctaa agtagactaa ttgtaacaag
    24241 gatttaaaaa aatttaaact atgtaagact tgcccctata cttatctgtt cttgctgtgt
    24301 tactatctgt aaagagaatt ttcgtgtgaa attcatgacg atagttgaaa atattgctta
    24361 atttgttata ataaaaagta agcagaaata aaaaggttgg gcgtaagctt caaggatcta
    24421 gttcttcaag gggtgagaag gacattttat tgaaggggcc tgcctatact aagttatctt
    24481 tagaggtaca tactttcaat gaaaaaagaa agcatgtacc tcttttttaa atcgaaagga
    24541 agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa gcagaagaag aaaatgagaa
    24601 gcgtgctcga accctacggc agcagttgaa gacctatgca gaagaaaaag agcaagaatt
    24661 ggagataatc caaacgaaca gtcggcaaca gctgaaacag ttggtagaag aaaaagaaca
    24721 attcgaaaag caagaattag aaaaagaaaa aatgaaattg aaagcagaag cagacaaggt
    24781 gcaagcaact ttgaaagacc aatatcaaaa gcatcagagt caagcaatcg gtgcaatcat
    24841 tgaaagggtg aaagaaactt atggccgtca ctaagatgga aaaagtgacc ctcatttcag
    24901 ataaaagaaa tcaggagagg attttacaag cagtacaagg aatccaaaat gttgaaatcc
    24961 gggatctgta tcaaaataga acaaacaacc agtgggtaga aaaatatttt ccagatacca
    25021 cgattattga taaagaagca aaattaagcg ctttaggcaa tcggctgaaa gacatccaag
    25081 aagctatcca attcatacaa caccacggcg atagccgtca aaagaaactc catttgaaac
    25141 gtcaagaact ttccttacaa gacttggaag caaattattc cgaagaaaat ttcatgaata
    25201 aattagaaga agtccttgat ttaaaagaaa agtgggaaca gctaactgaa aaacgtgatc
    25261 gtttagaaga agaggaagaa tggcttcttc attggcaaca gctggatatt gctccaagta
    25321 tgtatgcaag taagacgacc atttttgaga tggggactgt gagtgcagca aattttgaaa
    25381 gtttcaagga agaactaagt aagcttgatg acgtttatat ggaagaaata gattatacgc
    25441 caaaacaagt ttatctttcg tatgttgctt taaaaaaatc agttgctgct atagaagaaa
    25501 ttgctgggaa atacgggttc aataaagaaa cgtatcctta tgaacaaaat cctaaagaaa
    25561 aattgcaaga aaccaaacag caattatcca ttgttcacga gcagcaaaaa aagataacga
    25621 cagcgttagg tcagtgcagc ggctatatcc aagaatttga atgggtagaa gaagtcacac
    25681 ttgccgttgc agaacgggaa aaagtaaaag aacggtttat ccatgccact tatctgatcg
    25741 ttttgcaagg ttgggtagat gcagaagaaa aacagaattt gatgcatgtg ttaaacgaaa
    25801 ctgtatcaga agaagatata tacgttagtt tcgaaacacc aaactcagaa gagattcagc
    25861 aggaagtccc tactaaattg aaaaaccatc cgcttatcga accttttgaa ttgctgactg
    25921 aaatgtacag tctgccaaaa tatgaagaag tagatccaac gccttggatg acaccatttt
    25981 atcttgtctt cttcgggatg atggtcgcag atgttggtta cggattgctg atgctcattg
    26041 ggacaatact agctcaaaaa ctatttgttt taccccgcgg gatgaaacgt ttcgtaaaat
    26101 tctttgagat tctctcggta ccatcgatca tctggggatt gatctacagt tcatttttcg
    26161 gacaggcatt accaaaagaa ctatttggta tccgcttgcc tttcccaatc ctatctacga
    26221 cggatgacgt caatacgatt ttgattttat ccgtgatttt cggtctgata cagatcttgg
    26281 ttggattatt cgtggcagca aaggaaaata tcaggaggaa agattacttg agtgctgtca
    26341 gtgatggttt tgcctggcaa gggatcctga ttgggatcgt tattgcattg attggtgtca
    26401 tgttgataaa aaatacagca tttgtttatc ttggcgcggg gctatcgatc attgctgctt
    26461 tatgtatctt aatcgtgccg attatccaat cgccgtctaa agtcaagggt gctgccaaag
    26521 gagcgtataa tctttacgga ttgactggtt atatcggtga tttagtcagt tatacacgtt
    26581 tgatggcact aggtatttca ggcggaagta ttggcgcggc attcaatatg ctagttgctt
    26641 tcatgccgcc agccgctcga ttcagtgtag ggatcctttt gatcatcgct ttacacgcac
    26701 tgaatatgtt tttgacactg ttaagtgcct atgtccatgg tgcacgattg cagtatgtcg
    26761 aattttttgg aaaattttat acaggaggcg gacgtgcctt tgacccgctg aaaacggcgg
    26821 aaaaatacgt caacatcaat cataagaaaa aaaacaataa ataattggag gatttttata
    26881 caatgatgga ttacttaatt agtcaaaatg gtggaatggt atttgcagta ttagcgatgg
    26941 caacagcgac gattttttca gggattggtt cagctaaagg tgttggaatg acaggtgagg
    27001 cagcagcagc gttaacaaca agtcaacccg aaaaattcgg acaggctttg attctgcaat
    27061 tgcttccagg tacgcaaggg ttgtatggat tcgttatcgc ctttttgatt tttatcaact
    27121 taggaaatga tatgtcagtt gtacaaggat tgaactttct aggcgcttct ctgccgattg
    27181 cttttacagg tttgttttca ggtatcgccc aagggaaagt tgcagcagca ggtatccaaa
    27241 ttttagcgaa aaaacctgaa catgctacaa aaggaatcat ttttgctgcg atggttgaaa
    27301 catatgcgat tttaggtttc gttatttcct tcctattagt attgaatgcc taagcataaa
    27361 ggaggaaaag cagatgaacg ccattgaaaa aatcatctct cagatgaatg aagcagcaga
    27421 gcaagaaaga gcggcgttag agcaagaaga gcgaatgaag atcgatcaaa actttgaaca
    27481 aaaacgtacg caaatcgaaa cagaacatca aaaacaaaaa gaaaaacaaa tcgaattact
    27541 tgaaaaaaaa taccgtcagc ttaggaatcg ccaacaagta gaagtacgac aagaaaattt
    27601 gaatgcgaag caggaatttt tacgacgatt gtttgcagat gcaatagctg aaatggagaa
    27661 ttgggatgaa tctgaacaaa tccagtttat caaaaattca ttgtattcat tacctttgac
    27721 aggcaaagta acattcattg ctggagaaaa atcagcagct tatctttctc agacattgct
    27781 ggatgaatgg aacaatgaac ttccctttat gatggtttta agtgatgaaa ctgttgctga
    27841 tcaagcgggt tttctgataa acgatcaagg agttcagtat aattttcttt tcagcagcct
    27901 tgtacaagat atccaaggaa cgatgagttt tgagattgcc aatcaattat tcgaataagg
    27961 agggatcagt gtatggaata ccacgaacta aatcccttga ttcgcggaag agagcttgaa
    28021 cttttgtcca atgagacatt tgaacggatg attcaaacaa attctgtcga agcacttggt
    28081 gaaatattga aatccacgat ttacagtcca tatatttatg acggatatga atatgacttc
    28141 gaacagaatc ttttaaagga gcagagccgt ctatttcgct ggttaaagga atcagcaccg
    28201 gagcctgaaa tcgtttggat ctatacgatg cgctatactt tccataatct gaaggttttg
    28261 acaaaagccg agatgacaaa tcaaaatctg gaccatttgt atatcgacga tggattttat
    28321 tcattagaaa ccttgaagga tgccattcat acgcaagttt caaatgaact ccctgaaagc
    28381 attatggaca gtatacgaga agtacgtgat tattttgaag agtctaccat tcttcaggga
    28441 attgacgtga tttatgatcg acagtttttg acagagcagc gacggcttgc cgataagcta
    28501 ggatatcaag aattgctgac agaagtcatt gcttttattg acttgaccaa tatcacaacg
    28561 atggctcgag gaatattgca gaaaagaaca tatgggttca tgacgacggt actttccagc
    28621 tcgggaagta tcgaaaaaga agtatttttg aactttgtag ataaagatat ggaagattac
    28681 acgaattttc tattagagac agattatgct gattttctgc gccctgtcat ccgtgaggga
    28741 aatcttgatc tcgtaaagct tgaacagttg aaagatgatt atctaagttc attgtatcaa
    28801 gaagcacaaa cccaggcttt cggaccactg ccactactag cctttttgaa cgcaaaagag
    28861 gtagaaagca aaaaccttcg tttactggtt atcggcaaaa gaagccattt cgaaccagaa
    28921 acaatcagaa agcgggtgag acaagtctat gacgcataag atcggtgtag ttggcgacaa
    28981 agattctgtt ttgccattca aattgtttgg ctttgatgtt cgttatggta caaccaagca
    29041 aaccgttcgt caggcaattg aagaaatggc aaagaatgag tatggcgtga tttatgtcac
    29101 agagcaatgt gcagcattga gcccagaaac gatcgaacgt tacaaggaac aaatgatacc
    29161 tgctatcgta ttgattccca atcatcaagg atcattaggg atagggctgg aagaaattca
    29221 aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg agatagaaat
    29281 tttgcaaatt ggaaaaatca taaaagtctc cggacctctt gtaatggctg agaacatgtc
    29341 tgatgccagt atccaagaca tgtgtctagt gggagatcta ggagttatcg gagaaattat
    29401 cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa gagacttctg gaattggtcc
    29461 aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt gaattaggac cagggatcat
    29521 ttcacaaatg tttgacggaa tccaacggcc attagatacc tttatggaaa tcacacaaag
    29581 taatttctta gggcgcggag ttcaattgcc cgcattggac catgagaaaa aatggtggtt
    29641 cgaaccaaca gtagaagccg gagaaacggt gagtgctgga gatgttatcg ggatcgtaga
    29701 agaaacaaaa gttataaaac ataaaatcat ggtgccaaat gggataaaag gaacgatcaa
    29761 aacaatcgaa aaaggttcat tcactattga tgaaacagta tgtgtcattg aaaccgaaaa
    29821 tggtgacaaa aatttgacga tgcttcaaaa atggccagta agacgtagtc ggccaatcaa
    29881 agaaaaatta aacccggatg cacccatgat cactggacag cgagtgattg atacattctt
    29941 cccagtgaca aaaggaggag cagcagctgt acctggtcct tttggtgcag ggaaaacagt
    30001 cgttcagcat caaattgcaa aatgggccga tgtggacatg gttgtctatg tgggatgtgg
    30061 cgagcgtgga aatgagatga cggatgtttt aaatgaattc cctgaattga ttgaccctaa
    30121 tacaggagaa tcgttgatgg aacgtacggt attgattgct aatacatcaa acatgccagt
    30181 agcagctcga gaagcttcta tctatacggg aatcacaatt gcggaatatt tccgtgacat
    30241 gggttatgac gttgctatca tggcagactc cacttcacga tgggcagaag cacttcgtga
    30301 gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt tatccagctt atcttggttc
    30361 acggcttgcg gaatactatg aacgatctgg aagagtcatt gcattaggat cagaaggtcg
    30421 cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct ggaggggata tttcggaacc
    30481 agtcacacaa aatacattgc gggtcgtaaa agtattctgg ggtcttgatt ccagtttagc
    30541 ccaaaaaaga cattttcctt cgatcgattg gatacaaagc tattctcttt attcaacaga
    30601 agtagggaag tatatggatc atatccttca agctgactgg gcatccatgg tcaccgaggg
    30661 catgcgtatt ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg tagggattga
    30721 ttccctttca gataatgacc gtctgacatt agaagtggca aaatccattc gtgaagatta
    30781 tttgcagcaa aatgcttttg atgatgtcga tacattcact tcaagagaga aacaattcaa
    30841 aatgttgaag atgatcttga cctttggtga agaaggacgc cgtgcgttgt cgttaggtgc
    30901 ttatttaagt gagatcatgg acggtaccgt atcgattcgt gaaaggatcg gacgaagcaa
    30961 atatatcccg gaaacagaat tagaaaaact agatcaaatt attggtgaaa tcaagcaaac
    31021 gatgcaaacg atcgtttcag aaggaggcat gagcaatgat taaagaatat cgtacgatcg
    31081 gtgaagtcgt cggtccattg atggcggttg aaaaagttgc tggtgtcaaa tatgaagaat
    31141 taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg gaagtccaag
    31201 aagataaagc aatggtccaa atattcgaag gaacaagtgg tatcaattta cgtgactctt
    31261 ctgtacgatt tttaggccat cctttagaac taggtgtatc ggaagacatg atcggtcgag
    31321 tattcgatgg tttgggacga ccgaaagata acggaccaga gatcctgcct gaaaagcatt
    31381 tggatattaa cggagaagta atcaatccag tagctcggga ttatccagat gagtttatcc
    31441 aaacaggtat ctctgcaatc gatcatttga atacattagt tcgtgggcaa aaattaccag
    31501 tattttctgg ctctggtctt cctcataaag aattagctgc tcagatcgcg cgacaagcaa
    31561 cggtactcga ttcatcagat gatttcgccg tagtctttgc tgctattgga atcacttttg
    31621 aagaagctga atttttcatg gaagattttc gtcaaacggg cgcaatcgac cgttctgtca
    31681 tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat cgctactcct cgtatggctt
    31741 tgactgctgc agaatatctt gcttatgaaa agggaatgca tgttttggtt atcatgacag
    31801 acatgaccaa ctatgcagag gcattgcgag agatttctgc agcacggcga gaagttccag
    31861 gaagaagagg atatcctggg tatctttaca cgaacttagc tactttattc gaacgagcag
    31921 gaagaatccg cggactaaaa ggatcggtta ctcaaattcc gattctaaca atgccagaag
    31981 acgataaaac tcatccaatc cctgacttga ctggttatat tacagaagga cagatcatct
    32041 tgactagaga gctttacaag agtgggattc agccaccaat tgatgtactc ccgtctcttt
    32101 cgcggttaaa agataaggga actggacccg gaaagacaag agaagaccat gcagctacga
    32161 tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta gccgtagttt
    32221 taggtgaatc tgctttatct gatgtagaca agatctatgc gaagtttgct gagcggtttg
    32281 aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc gattactgaa acacttgatc
    32341 tagggtggga actgctatcg atgttgccaa gaaccgaatt aaaacgaatc aaagacgaca
    32401 tgctggacaa atatctgcca gaagggaagt gagttctgat ggcacgattg aatgtgaatc
    32461 cgacccgcat ggaattgact cgattgaaaa aacagttgac aactgccaca agagggcata
    32521 aattattaaa agataagcaa gatgaactga tgcgtcaatt catcctgttg attcgaaaga
    32581 acaaccagct gcgtcaagaa atggaagagt ctatgacaga agcaatgtct gattttgtat
    32641 tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt atttgttttg ccggcggaaa
    32701 acgttgattt gtctatcgta gacaaaaata tcatgagtgt aaaagtacct gtgatgaatt
    32761 ttgaatatga tgaacagctg acagacacac ctttggaata cgggtatttg aattccagtg
    32821 ccgaactgga tcggtcaatc gatcggttta ctaccctgct tccaaaactt ttggaactga
    32881 cagaaatcga aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca cgtcggcgag
    32941 tcaatgcatt agaatatatg acgatccctc aattagaaga aacgatctat tatattcgta
    33001 tgaagctgga agaaaacgag cgcgcagaag taacaaggtt gatcaaagta aaaaatatgg
    33061 gtaccactga ctaagtggta tcttttttta aaagaggaaa acgatgatta aacaaagcaa
    33121 acaaataaag aaacgtttgc cggctgttca gcttctcgca ttaggatttt ttctcctgat
    33181 tttagttggc ggcggcctac ttaccctacc attttttagt aatagtgggc atgggacaaa
    33241 ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat tgacaacatt
    33301 gaatacagca gagcattgga acagtgctgg tcaatttttg atcatgcttt tgattgagat
    33361 cggcggtctt ggttttatga tggttccgat tctatgcttt gcattgataa agaagaaagt
    33421 cagtctcagc acacgtatta tattaaaaga agcgctgaat ctagaagaag tatcaggtgt
    33481 gatgaagctc atgctttaca tcctaaaatt ggcctttgtt atccaattga ttggtacggt
    33541 tgctttatct tttgtatttg tgcctgaatt tggctggttg aaaggtatct ggtacagttt
    33601 gttccatgca atctctagtt tttgtaatgc agggtttgat ttgttaggag acagtttagc
    33661 caatcaccag caaaatgttt atttgatcat ggtggtatct gctttgatca tttctggtgg
    33721 gttaggcttc atcgtatggc gtgatttact ggaatatcat cagaaaaaga agatgacgat
    33781 tcattcaaaa atcgcattaa ccgttacagg taccctttta ttaggcggat tcgttgtgtt
    33841 ctttttcaca gagcgcaatg gggcaacgct agtggaaggg aatatgtttc aacgattggc
    33901 aaacaccttt tttatgagtg tgacacctag aacggctgga tattattcag ttgattacct
    33961 ccatatgagc catgcaggtc tgattttaac gatgtttttg atgtacatcg gcggaacatc
    34021 tgggtcgacg gccggcggat tgaaaacaac tacgttaggt gtgttgctga tccagatgcg
    34081 ttcgatgttg aaaggacgta cgagagcgga agcatttggc cgtaccatcc gacaaggcgc
    34141 agtgttgcgg gcacttactt tattttttgt tacgttgacg ttgtgtgtag tcgccatcat
    34201 gattttatcg gtgactgaaa cgatacctaa aacttctggc atcgaatata ttgcttttga
    34261 ggtcttctct gcgtttggta cagtaggtct tacgatggga ttgacgccag acttgactgc
    34321 aatagggaaa ttgatcatta tagcgcttat gtacattgga cgagttggtg tattgactgt
    34381 ggtcttctct ctgatgacaa aagcacaaag aacacaatca aaatacaaat atccagaaga
    34441 aagtgtcatg attggataat ttcatcttct gacagaaacc tagtgattta gtaaaataag
    34501 aaaataaaat aatttttctt gcttcttgga tactttaagt gcaaaatgtt cgtttatttt
    34561 ttgaggcact acccgttgtt gaacctcggg tagtgcctct tttttattga aaggaatcaa
    34621 taagatttga ttcctttatt tatcaatagg ttataattca tattttttta ttttttcttt
    34681 tacgatactt agtccagaag ctaaaatttc aaggttatta gaggataggg aaattcgaac
    34741 gaatgcatct ggttgcgcac gtgtagtaaa gcgtctggaa tgataaagtc ggataccgtt
    34801 agcaagaaag tcattctcaa ttatcttagg gtcaatctca ggatcaactg gaagaattcg
    34861 atagtaaggg acacgattcg ttggtttttc tactttgaaa agagaatcaa aaagctgatt
    34921 ggctcttttt gtaagactta atttagtttc taagatccta tttgcttcat ttgaatggag
    34981 gatttgagtg atgacttcca catctaatcc agaagttttc acatttatat taaaaattgc
    35041 ttgttctatc ttgctttgga catgtactgg aactacaaga taagcaactc gcaatccaga
    35101 acaaataaat tttgtcattc cagctaagta gattgtttgg ttaggtaata aattttggaa
    35161 aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat gaatggatat catcttcaat
    35221 aacccataga ccttcttttt caatcacttc ggctaattgc tctcgtcttt tgtgagagat
    35281 ttgaaaccct ataggattat tacaagaagg cataagaaag acaccatgaa tcttcttttt
    35341 tctacattcc tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat caattggaac
    35401 gatctctaaa tgatataact gagccaattc aatgaaatta gcgtatgtat atcggtctgt
    35461 tgctattcta tcacctggag agaaaagtgc agaaagtgtg atagccaatc cattttgaac
    35521 accggaaaca atagatagag attctaagga cggaatgact ccttgagttt ttaaccaatt
    35581 tgctgcagta gtcaattgaa aatttgtgcc tttgggatct tggtaattaa gcaaaccaac
    35641 aagatttggg ttattactag ttgattgaat aaacgggaca acgaatttat tacattcttc
    35701 aaaagaacta accagaccaa aatcaatgat atgttcggtt acttcattgg tagagatggt
    35761 gatggactca aagacattgg gagaaataaa tgtgccactt ccaatattcg tatacagcaa
    35821 acctttctca attccatatt tataagcctt gccaacggta gtaaaattta aatctaaata
    35881 atcagctagc tctctttggg agggtaactg agtgtttttc tgtaatttat tctttaaaat
    35941 atcttgttct aattgttgaa tcaacgatcg ataataagga cgcttcaatt tttgtttgtc
    36001 tggtcgccaa gttaacgggt aattgtcata agaattaata ggcattttct ccactcctta
    36061 aaattgtata ccatacaatt ttaccattga ttgtattgat tttagaagtt aatcttttgt
    36121 gaggagggaa ttgtctatga aaattattaa ttttgaggaa gtagtagctt cttttagttt
    36181 tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg gtaaaatcag
    36241 ccaaaatcct cgattggtgg aaattttacc tgacggaaaa ggcaaaaata tgtttgcgat
    36301 gatgcctgct tatttggggg agaacagata ttttggcaca aaagtcatta ccgcttttcc
    36361 ggataatcat cagtatcatt taccttctca tttaggagag attttcttgt ttgatgctca
    36421 aaacggacta cccaaagcat tgataaacgc aaatgcagtc acctggattc gaacagctgc
    36481 tgtatctgcg ctggcaacag attacttagc aaatccgcat gcagaaacac ttgctttaat
    36541 tggagctggt caacaagcat tgtcgcattt gactgcgatt tctaccattc gtcccatcac
    36601 tacagtgttt gtttacgata ttttagaaga aagacgaaat acttttatcg ctaaagctaa
    36661 aaaacagtat cctaaaataa cgtttataaa tgcaatatca gtagaagatg cagtacgtcc
    36721 agcggaaatc atttgtacgt tgacctctag taaagaggca tttcttcaaa aagagtgggt
    36781 aagggaagac gcacatatca atgctgtggg cacatttact cctgatacaa gagagataag
    36841 tacatcgttg atggaaactt ctcaaatcta tgtagatgat tactcagctg ccttaacaga
    36901 aagcggggac ctgcttatag ccatggcaga aggagattta gaggaatctt gtattgttgg
    36961 tacattaggc gaattagttt ctgagaaaaa aacattttat aaaggagaat cgaggccgac
    37021 gattttcgac gcagttggtc tagctattga agatctttgt tgtgcagaat atatttataa
    37081 caaattagag aaaggggctt taaaatgaac ttagattatt acgtagttga tgcatttgca
    37141 gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg gctgccagaa
    37201 ggtacgatgc aaaaaattgc cattgaaaac aatttatctg aaacagcatt tacagtgaag
    37261 aaaaaccaag aatttgagct tagatggttt acgccagatc gagaaatcga tctttgtggt
    37321 cacgcgacat tagcaacagc atttgtttta tttaactatt acaaaatacc tgatgaaacg
    37381 attaaatttt ctactcaaag cggtaatcta tttgtgacaa gaattaaaga ttattattac
    37441 atggattttc ctagtattat gccgaaaaaa gtgccgatat tgtctgaata tgaagaagcg
    37501 attggggcta agattaaaga agcctactta gcacgtgatt tatttttcgt attagaagat
    37561 gaaaaaacgg tggctaaatt gcagccagat tttacagcta tcaaaaattt tgaattagga
    37621 ataggagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag aaccttcttt
    37681 ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa tcttattcct
    37741 tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc atcaaggggc
    37801 ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa agcagttttg
    37861 ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat aaattggtgg
    37921 agagtggcaa ccttatagct acaatggttg ttaaaaaaag acgcaaactt tttcaattta
    37981 atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa tcaagagtaa
    38041 aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag ggtcaatgac
    38101 aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag tcagtaatct caagaccggc
    38161 agctttatgt tgctcgtgta atttttctag atcatcagct gctatagcga tatgaccata
    38221 gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta attcatagtc
    38281 atccccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat cacggcggcg
    38341 gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa gatctttcac
    38401 tcgaacacat gtgtgagcca ttttcataga ttcatctctc cttttccttt tactcaatca
    38461 taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt catgagaaat ttctcatgta
    38521 aaatggatag aaaataaaac tgttaaggtg gaaactattc atggataaac caatttttgg
    38581 tgaaaaaaag caatttgctg attatcaaac ccgttatgca gcatatattg tcatagaaag
    38641 agatcagcag attgcagtaa tcgaggctcc aaatggtgct ttttttcttc ctggcgggga
    38701 aattgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag aagaaatggg
    38761 aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt actcgaatta
    38821 tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt ggaagcgtat
    38881 aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag aagcgcttgt
    38941 gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa aaaataaaaa
    39001 ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac agtacatatt
    39061 ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca tttgctgctt
    39121 ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat tcaatcatgg
    39181 ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata ccaaggtgct
    39241 gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact tcgaagtaat
    39301 agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa cgcgcaattg
    39361 ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc atgtctggtg
    39421 tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct tcgtggatcg
    39481 tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc gaatggagat
    39541 gactgccgac acggcgtgcc atcacttcga tctctaggaa atcttcatgt gtcagtccgt
    39601 gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta taagtaataa
    39661 cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca cctggtaagg
    39721 gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca atcgcttctt
    39781 ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg tcgatagcga
    39841 ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc aatgcgccat
    39901 tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa tgatcacgca
    39961 aggacggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt tcttgtcccg
    40021 ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg tcatttcgca
    40081 aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca taaggtagat
    40141 cgtagctaga attcacagca gtgtaataaa caggattata tccgaccttt tctactaaca
    40201 aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca gatttcagtg
    40261 gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca gattcagacc
    40321 attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct tctaaagtaa
    40381 agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg ctggcatctt
    40441 cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc ataccacggg
    40501 aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata taatcttcca
    40561 ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga atcacacgta
    40621 atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca tcccgaagca
    40681 aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact tgtgggttgg
    40741 ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct actcggttaa
    40801 gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg gcttcatgat
    40861 tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca tcatgcaaca
    40921 gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg cctatttttt
    40981 cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca taaacgccta
    41041 atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa gtgcctaatt
    41101 gtttaatgcg tcgtagacgt tggacttctt tcgaattgat caaatctaag attacttgat
    41161 gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata ggcagcattt
    41221 gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg ttccattctc
    41281 tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc ggtagaatcc
    41341 tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc tttgacttct
    41401 tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc aggatccact
    41461 gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa tcgcttcatg
    41521 acttgcccgc gtttttcctg attaccgttg acacttaagt acatcataac agaaatccca
    41581 tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct taaatcataa
    41641 gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt tggaaaagcg
    41701 tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt ttttgtgtca
    41761 ctttggggga aaatcaaaga aatattcaaa attccttcat ctgatacgat ccctaatcca
    41821 cctgaatttc tgatcactgg agcgtaacct gcatctttca gtgtttggat cccgtcactt
    41881 agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata ttgccagaaa
    41941 tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt taatgcaaaa
    42001 ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg gttcattcaa
    42061 ccggctcctt tgtataaatt catattttct acaatattat actctaaaat caaagagttg
    42121 ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca aaagaaacta
    42181 ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca caaggaacac
    42241 cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa gatttttatt
    42301 ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt tataaagaga
    42361 ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca gatggtcatg
    42421 tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat aaagaacggt
    42481 tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt acgaaagaac
    42541 tgcatgttag tttgaaagac cgtcccacag gagggaaggt tcgaatcgat tatgatctgt
    42601 tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca gcgtaaatat
    42661 aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga ctgtgaaagg
    42721 acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa ttatcaatga
    42781 tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt tctgatttag
    42841 ctaaccagat ccaaaactat cttgggaaat cagacagtga aattcgtgat tcactcgcac
    42901 aattttatac tgacttgaat attgacggca gctttatctc attaggggat aatcgttggg
    42961 gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat ggaatggacg
    43021 aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc tttatcaatg
    43081 atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat ctgacagaag
    43141 aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc gctgcttata
    43201 attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa gacttgccag
    43261 gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa gattatgaag
    43321 aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag aggttgtgac
    43381 agagacgatc agcctatcag aataaggagg tgtgacacga ttt
//
LOCUS       DAZUMB010000008        43369 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000008 DAZUMB010000000
VERSION     DAZUMB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.008"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..2389)
                     /locus_tag="ACG80L_000344"
     CDS             complement(8..2389)
                     /locus_tag="ACG80L_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5022635.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="ACG80L_000345"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="ACG80L_000345"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS5022636.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="ACG80L_000346"
     CDS             3090..3629
                     /locus_tag="ACG80L_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS5022637.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="ACG80L_000347"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="ACG80L_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS5022638.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="ACG80L_000348"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG80L_000348"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="ACG80L_000349"
     CDS             4736..5902
                     /locus_tag="ACG80L_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS5022639.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="ACG80L_000350"
     CDS             5948..7444
                     /locus_tag="ACG80L_000350"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS5022640.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="ACG80L_000351"
     CDS             7525..8208
                     /locus_tag="ACG80L_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5022641.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="ACG80L_000352"
     CDS             8853..10043
                     /locus_tag="ACG80L_000352"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS5022642.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="ACG80L_000353"
     CDS             10145..11098
                     /locus_tag="ACG80L_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS5022643.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="ACG80L_000354"
     CDS             11167..12516
                     /locus_tag="ACG80L_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5022644.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="ACG80L_000355"
     CDS             complement(12699..13022)
                     /locus_tag="ACG80L_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022645.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="ACG80L_000356"
     CDS             13151..14314
                     /locus_tag="ACG80L_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5022646.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="ACG80L_000357"
     CDS             14384..15733
                     /locus_tag="ACG80L_000357"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5022647.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="ACG80L_000358"
     CDS             complement(15778..16209)
                     /locus_tag="ACG80L_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5022648.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="ACG80L_000359"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="ACG80L_000359"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS5022649.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="ACG80L_000360"
     CDS             19439..20035
                     /locus_tag="ACG80L_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5022650.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="ACG80L_000361"
     CDS             20157..21305
                     /locus_tag="ACG80L_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5022651.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="ACG80L_000362"
     CDS             complement(21343..21756)
                     /locus_tag="ACG80L_000362"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS5022652.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="ACG80L_000363"
     CDS             21835..22266
                     /locus_tag="ACG80L_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS5022653.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="ACG80L_000364"
     CDS             complement(22365..22598)
                     /locus_tag="ACG80L_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5022654.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22781..24451
                     /locus_tag="ACG80L_000365"
     CDS             22781..24451
                     /locus_tag="ACG80L_000365"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS5022655.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24598..24984
                     /locus_tag="ACG80L_000366"
     CDS             24598..24984
                     /locus_tag="ACG80L_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022656.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24971..25669
                     /locus_tag="ACG80L_000367"
     CDS             24971..25669
                     /locus_tag="ACG80L_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS5022657.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25674..26138
                     /gene="lspA"
                     /locus_tag="ACG80L_000368"
     CDS             25674..26138
                     /gene="lspA"
                     /locus_tag="ACG80L_000368"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS5022658.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26135..27046
                     /locus_tag="ACG80L_000369"
     CDS             26135..27046
                     /locus_tag="ACG80L_000369"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5022659.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27281..27820
                     /gene="pyrR"
                     /locus_tag="ACG80L_000370"
     CDS             27281..27820
                     /gene="pyrR"
                     /locus_tag="ACG80L_000370"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS5022660.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27834..29117
                     /locus_tag="ACG80L_000371"
     CDS             27834..29117
                     /locus_tag="ACG80L_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS5022661.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29130..30056
                     /locus_tag="ACG80L_000372"
     CDS             29130..30056
                     /locus_tag="ACG80L_000372"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS5022662.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30075..31355
                     /locus_tag="ACG80L_000373"
     CDS             30075..31355
                     /locus_tag="ACG80L_000373"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS5022663.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31360..32436
                     /locus_tag="ACG80L_000374"
     CDS             31360..32436
                     /locus_tag="ACG80L_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS5022664.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32441..35626
                     /gene="carB"
                     /locus_tag="ACG80L_000375"
     CDS             32441..35626
                     /gene="carB"
                     /locus_tag="ACG80L_000375"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS5022665.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35680..36456
                     /locus_tag="ACG80L_000376"
     CDS             35680..36456
                     /locus_tag="ACG80L_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS5022666.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36453..37385
                     /locus_tag="ACG80L_000377"
     CDS             36453..37385
                     /locus_tag="ACG80L_000377"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS5022667.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37382..38098
                     /gene="pyrF"
                     /locus_tag="ACG80L_000378"
     CDS             37382..38098
                     /gene="pyrF"
                     /locus_tag="ACG80L_000378"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS5022668.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38105..38731
                     /gene="pyrE"
                     /locus_tag="ACG80L_000379"
                     /pseudo
     CDS             38105..38731
                     /gene="pyrE"
                     /locus_tag="ACG80L_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            38758..39036
                     /locus_tag="ACG80L_000380"
     CDS             38758..39036
                     /locus_tag="ACG80L_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022669.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39074..39715
                     /locus_tag="ACG80L_000381"
     CDS             39074..39715
                     /locus_tag="ACG80L_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS5022670.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39758..40450
                     /locus_tag="ACG80L_000382"
     CDS             39758..40450
                     /locus_tag="ACG80L_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS5022671.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40447..41349
                     /locus_tag="ACG80L_000383"
     CDS             40447..41349
                     /locus_tag="ACG80L_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5022672.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="ACG80L_000384"
     CDS             complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="ACG80L_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS5022673.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13875 a         7663 c         9440 g        12391 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22741 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    22801 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    22861 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    22921 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    22981 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23041 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23101 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23161 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23221 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23281 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23341 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23401 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23461 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23521 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23581 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23641 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23701 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    23761 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    23821 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    23881 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    23941 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24001 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24061 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24121 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24181 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24241 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24301 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24361 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24421 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24481 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24541 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24601 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24661 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24721 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    24781 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    24841 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    24901 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    24961 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25021 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25081 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25141 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25201 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25261 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25321 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25381 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25441 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25501 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25561 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25621 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25681 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25741 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    25801 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    25861 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    25921 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    25981 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26041 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26101 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26161 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26221 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26281 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26341 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26401 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26461 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26521 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26581 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26641 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26701 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    26761 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    26821 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    26881 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    26941 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27001 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27061 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27121 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27181 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27241 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27301 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27361 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27421 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27481 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27541 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27601 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27661 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27721 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    27781 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    27841 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    27901 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    27961 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28021 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28081 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28141 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28201 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28261 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28321 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28381 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28441 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28501 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28561 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28621 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28681 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28741 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    28801 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    28861 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    28921 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    28981 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29041 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29101 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29161 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29221 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29281 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29341 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29401 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29461 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29521 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29581 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29641 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29701 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    29761 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    29821 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    29881 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    29941 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30001 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30061 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30121 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30181 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30241 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30301 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30361 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30421 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30481 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30541 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30601 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30661 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30721 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    30781 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    30841 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    30901 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    30961 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31021 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31081 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31141 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31201 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31261 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31321 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31381 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31441 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31501 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31561 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31621 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31681 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31741 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    31801 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    31861 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    31921 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    31981 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32041 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32101 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32161 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32221 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32281 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32341 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32401 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32461 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32521 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32581 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32641 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32701 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    32761 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    32821 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    32881 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    32941 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33001 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33061 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33121 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33181 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33241 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33301 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33361 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33421 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33481 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33541 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33601 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33661 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33721 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    33781 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    33841 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    33901 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    33961 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34021 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34081 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34141 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34201 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34261 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34321 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34381 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34441 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34501 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34561 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34621 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34681 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34741 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    34801 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    34861 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    34921 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    34981 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35041 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35101 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35161 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35221 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35281 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35341 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35401 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35461 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35521 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35581 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35641 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35701 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    35761 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    35821 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    35881 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    35941 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36001 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36061 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36121 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36181 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36241 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36301 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36361 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36421 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36481 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36541 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36601 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36661 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36721 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    36781 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    36841 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    36901 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    36961 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37021 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37081 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37141 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37201 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37261 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37321 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37381 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37441 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37501 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37561 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37621 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37681 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37741 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    37801 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    37861 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    37921 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    37981 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38041 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38101 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38161 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38221 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38281 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38341 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38401 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38461 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38521 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38581 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38641 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38701 atggaaaaaa gatcctgaaa attggcagat tgaagcgcgc ttgatgaaga tagagaaatc
    38761 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    38821 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    38881 aaattcattt ttctaagact ggttagcaga aagaagagaa taatctcttc tcttttttct
    38941 attatatata gattttctct attttttggt aagggagctg tttcaaaaat ggtaaagagc
    39001 ggaaatgttg cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag
    39061 ggaggtaact tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat
    39121 cacaagggca cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg
    39181 tgtagcatct attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga
    39241 ttggaaactg ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga
    39301 tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga
    39361 agaaaagatg acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta
    39421 tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc
    39481 agataaggtt gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa
    39541 agtttataag gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc
    39601 ttctgttcag tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg
    39661 aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg
    39721 atatgagatg cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc
    39781 tggagacaaa ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga
    39841 atttccattg cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga
    39901 cacgctttcc tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat
    39961 tcatttagta ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt
    40021 gacagatatt catttccact tgccttctga acatatcatc aacggaaaac aggaagatat
    40081 tgaatttcat tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta
    40141 ccagctaatg gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt
    40201 agaaaaacat gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt
    40261 ccattatatt ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat
    40321 ggatcatgta ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg
    40381 accgaacaac cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt
    40441 tgagcaatga atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc
    40501 agagaagcgg caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa
    40561 ttagaaaccg agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca
    40621 gaagaagggc aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta
    40681 gaaactcact atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat
    40741 gatttcttag gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa
    40801 tttcggatca tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt
    40861 gagatcggca ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa
    40921 caaggagagc taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa
    40981 ggacatccat tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa
    41041 gttcgtttta cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc
    41101 cctgatcaag aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt
    41161 gaaactgatg cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg
    41221 cggttaatcc ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa
    41281 cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag
    41341 cagagctaag aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg
    41401 ggttgtgaca gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg
    41461 ctgttgtggg aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt
    41521 tcatagctgt aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa
    41581 taacataacc aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa
    41641 ccggcacttg ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg
    41701 taattgtttc atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt
    41761 ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa
    41821 gattgttttt accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag
    41881 atggtttctt acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca
    41941 attcttcttt aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag
    42001 attcaagata ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag
    42061 cgttcttcag cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg
    42121 ccggatctag tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt
    42181 cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt
    42241 aattctcagc gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat
    42301 ttcgttttag ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat
    42361 ctttctctgc tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg
    42421 ttacagcaga tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat
    42481 tttccgtttc gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc
    42541 atacaaccat cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat
    42601 cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa
    42661 gccgctgaaa gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg
    42721 gagcaacata ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt
    42781 gtttgatcgc atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac
    42841 gtcccattaa ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag
    42901 caaagtggaa atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc
    42961 catccaaata ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat
    43021 cgatttgggt aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt
    43081 ttttgccgcg tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg
    43141 agatccggcc agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac
    43201 catcaaagga catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa
    43261 aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta
    43321 attacataaa aaacagtgtt tctatcgttg aaatccaaat ttttaggta
//
LOCUS       DAZUMB010000009        43133 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000009 DAZUMB010000000
VERSION     DAZUMB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.009"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="ACG80L_000385"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="ACG80L_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS5022674.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="ACG80L_000386"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="ACG80L_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS5022675.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="ACG80L_000387"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="ACG80L_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS5022676.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="ACG80L_000388"
     CDS             1640..2272
                     /locus_tag="ACG80L_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5022677.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="ACG80L_000389"
     CDS             complement(2274..3179)
                     /locus_tag="ACG80L_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5022678.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="ACG80L_000390"
     CDS             complement(3264..3923)
                     /locus_tag="ACG80L_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5022679.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="ACG80L_000391"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="ACG80L_000391"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS5022680.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="ACG80L_000392"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="ACG80L_000392"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS5022681.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="ACG80L_000393"
     CDS             6166..7251
                     /locus_tag="ACG80L_000393"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS5022682.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /gene="fni"
                     /locus_tag="ACG80L_000394"
     CDS             7248..8312
                     /gene="fni"
                     /locus_tag="ACG80L_000394"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS5022683.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="ACG80L_000395"
     CDS             complement(8561..9364)
                     /locus_tag="ACG80L_000395"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS5022684.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="ACG80L_000396"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="ACG80L_000396"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS5022685.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="ACG80L_000397"
     CDS             complement(10242..11249)
                     /locus_tag="ACG80L_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5022686.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="ACG80L_000398"
     CDS             complement(11256..11900)
                     /locus_tag="ACG80L_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5022687.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="ACG80L_000399"
     CDS             complement(12000..12758)
                     /locus_tag="ACG80L_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS5022688.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="ACG80L_000400"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="ACG80L_000400"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS5022689.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="ACG80L_000401"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="ACG80L_000401"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS5022690.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="ACG80L_000402"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="ACG80L_000402"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS5022691.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="ACG80L_000403"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="ACG80L_000403"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS5022692.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="ACG80L_000404"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="ACG80L_000404"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS5022693.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="ACG80L_000405"
     CDS             18156..19025
                     /locus_tag="ACG80L_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5022694.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="ACG80L_000406"
     CDS             complement(19151..20050)
                     /locus_tag="ACG80L_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5022695.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="ACG80L_000407"
     CDS             complement(20215..21039)
                     /locus_tag="ACG80L_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5022696.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="ACG80L_000408"
     CDS             complement(21050..22345)
                     /locus_tag="ACG80L_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS5022697.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="ACG80L_000409"
     CDS             complement(22361..22840)
                     /locus_tag="ACG80L_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS5022698.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="ACG80L_000410"
     CDS             complement(22846..23874)
                     /locus_tag="ACG80L_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS5022699.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="ACG80L_000411"
     CDS             complement(23891..24997)
                     /locus_tag="ACG80L_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5022700.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="ACG80L_000412"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="ACG80L_000412"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS5022701.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="ACG80L_000413"
     CDS             complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="ACG80L_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS5022702.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="ACG80L_000414"
     CDS             complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="ACG80L_000414"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS5022703.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29968..30666
                     /locus_tag="ACG80L_000415"
     CDS             29968..30666
                     /locus_tag="ACG80L_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5022704.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30834..33473)
                     /locus_tag="ACG80L_000416"
     CDS             complement(30834..33473)
                     /locus_tag="ACG80L_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5022705.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34092..35123)
                     /gene="queA"
                     /locus_tag="ACG80L_000417"
     CDS             complement(34092..35123)
                     /gene="queA"
                     /locus_tag="ACG80L_000417"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS5022706.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35316..36491
                     /locus_tag="ACG80L_000418"
     CDS             35316..36491
                     /locus_tag="ACG80L_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5022707.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36495..37136
                     /locus_tag="ACG80L_000419"
     CDS             36495..37136
                     /locus_tag="ACG80L_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022708.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37133..38050
                     /locus_tag="ACG80L_000420"
     CDS             37133..38050
                     /locus_tag="ACG80L_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5022709.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38047..38718
                     /locus_tag="ACG80L_000421"
     CDS             38047..38718
                     /locus_tag="ACG80L_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022710.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39259..39747)
                     /locus_tag="ACG80L_000422"
     CDS             complement(39259..39747)
                     /locus_tag="ACG80L_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS5022711.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39948..40214)
                     /locus_tag="ACG80L_000423"
     CDS             complement(39948..40214)
                     /locus_tag="ACG80L_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5022712.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40214..40477)
                     /locus_tag="ACG80L_000424"
     CDS             complement(40214..40477)
                     /locus_tag="ACG80L_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5022713.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40998..41138
                     /locus_tag="ACG80L_000425"
                     /pseudo
     CDS             40998..41138
                     /locus_tag="ACG80L_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(41280..43103)
                     /locus_tag="ACG80L_000426"
     CDS             complement(41280..43103)
                     /locus_tag="ACG80L_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5022714.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT        12285 a         9380 c         7899 g        13569 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    27901 aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag
    27961 gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta
    28021 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
    28081 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
    28141 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
    28201 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
    28261 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
    28321 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
    28381 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
    28441 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
    28501 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
    28561 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
    28621 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
    28681 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
    28741 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
    28801 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
    28861 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
    28921 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
    28981 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
    29041 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
    29101 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
    29161 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
    29221 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
    29281 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
    29341 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
    29401 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
    29461 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
    29521 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
    29581 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
    29641 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
    29701 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
    29761 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
    29821 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
    29881 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
    29941 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
    30001 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
    30061 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
    30121 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
    30181 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
    30241 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
    30301 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
    30361 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
    30421 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
    30481 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
    30541 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
    30601 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
    30661 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
    30721 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
    30781 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
    30841 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
    30901 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
    30961 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
    31021 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
    31081 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
    31141 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
    31201 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
    31261 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
    31321 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
    31381 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
    31441 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
    31501 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
    31561 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
    31621 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
    31681 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
    31741 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
    31801 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
    31861 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
    31921 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
    31981 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
    32041 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
    32101 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
    32161 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
    32221 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
    32281 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
    32341 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
    32401 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
    32461 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
    32521 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
    32581 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
    32641 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
    32701 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
    32761 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
    32821 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
    32881 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
    32941 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
    33001 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
    33061 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
    33121 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
    33181 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
    33241 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
    33301 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
    33361 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
    33421 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
    33481 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
    33541 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
    33601 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
    33661 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
    33721 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
    33781 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
    33841 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
    33901 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
    33961 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
    34021 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
    34081 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
    34141 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
    34201 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
    34261 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
    34321 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
    34381 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
    34441 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
    34501 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
    34561 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
    34621 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
    34681 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
    34741 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
    34801 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
    34861 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
    34921 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
    34981 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
    35041 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
    35101 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
    35161 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
    35221 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
    35281 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
    35341 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
    35401 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
    35461 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
    35521 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
    35581 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
    35641 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
    35701 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
    35761 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
    35821 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
    35881 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
    35941 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
    36001 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
    36061 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
    36121 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
    36181 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
    36241 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
    36301 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
    36361 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
    36421 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
    36481 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
    36541 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
    36601 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
    36661 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
    36721 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
    36781 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
    36841 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
    36901 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
    36961 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
    37021 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
    37081 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
    37141 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
    37201 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
    37261 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
    37321 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
    37381 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
    37441 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
    37501 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
    37561 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
    37621 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
    37681 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
    37741 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
    37801 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
    37861 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
    37921 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    37981 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    38041 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    38101 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    38161 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    38221 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    38281 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    38341 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    38401 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    38461 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    38521 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    38581 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    38641 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    38701 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    38761 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    38821 cttatgggta aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    38881 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    38941 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    39001 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    39061 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    39121 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    39181 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    39241 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    39301 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    39361 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    39421 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    39481 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    39541 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    39601 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    39661 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    39721 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    39781 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    39841 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    39901 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    39961 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    40021 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    40081 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    40141 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    40201 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    40261 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    40321 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    40381 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    40441 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    40501 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    40561 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    40621 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    40681 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    40741 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    40801 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    40861 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    40921 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    40981 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    41041 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    41101 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    41161 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    41221 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    41281 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    41341 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    41401 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    41461 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    41521 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    41581 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    41641 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    41701 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    41761 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    41821 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    41881 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    41941 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    42001 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    42061 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    42121 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    42181 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    42241 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    42301 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    42361 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    42421 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    42481 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    42541 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    42601 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    42661 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    42721 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    42781 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    42841 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    42901 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    42961 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    43021 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    43081 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt act
//
LOCUS       DAZUMB010000010        42832 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000010 DAZUMB010000000
VERSION     DAZUMB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.010"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1192)
                     /locus_tag="ACG80L_000427"
     CDS             complement(89..1192)
                     /locus_tag="ACG80L_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS5022715.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1821..2291)
                     /locus_tag="ACG80L_000428"
     CDS             complement(1821..2291)
                     /locus_tag="ACG80L_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS5022716.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            2621..4261
                     /locus_tag="ACG80L_000429"
     CDS             2621..4261
                     /locus_tag="ACG80L_000429"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS5022717.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4415..5737
                     /locus_tag="ACG80L_000430"
     CDS             4415..5737
                     /locus_tag="ACG80L_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS5022718.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5805..6239)
                     /locus_tag="ACG80L_000431"
     CDS             complement(5805..6239)
                     /locus_tag="ACG80L_000431"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5022719.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(6304..7141)
                     /locus_tag="ACG80L_000432"
                     /pseudo
     CDS             complement(6304..7141)
                     /locus_tag="ACG80L_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(7883..8509)
                     /locus_tag="ACG80L_000433"
     CDS             complement(7883..8509)
                     /locus_tag="ACG80L_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022720.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8804..9139)
                     /locus_tag="ACG80L_000434"
     CDS             complement(8804..9139)
                     /locus_tag="ACG80L_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5022721.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(9126..9410)
                     /locus_tag="ACG80L_000435"
     CDS             complement(9126..9410)
                     /locus_tag="ACG80L_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS5022722.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9466..10989)
                     /locus_tag="ACG80L_000436"
     CDS             complement(9466..10989)
                     /locus_tag="ACG80L_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5022723.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10982..12157)
                     /locus_tag="ACG80L_000437"
     CDS             complement(10982..12157)
                     /locus_tag="ACG80L_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5022724.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(12161..12346)
                     /locus_tag="ACG80L_000438"
     CDS             complement(12161..12346)
                     /locus_tag="ACG80L_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022725.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(12312..14006)
                     /locus_tag="ACG80L_000439"
     CDS             complement(12312..14006)
                     /locus_tag="ACG80L_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5022726.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(14003..14476)
                     /locus_tag="ACG80L_000440"
     CDS             complement(14003..14476)
                     /locus_tag="ACG80L_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS5022727.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14545..14700)
                     /locus_tag="ACG80L_000441"
     CDS             complement(14545..14700)
                     /locus_tag="ACG80L_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS5022728.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14834..15214)
                     /locus_tag="ACG80L_000442"
     CDS             complement(14834..15214)
                     /locus_tag="ACG80L_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5022729.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(15218..15433)
                     /locus_tag="ACG80L_000443"
     CDS             complement(15218..15433)
                     /locus_tag="ACG80L_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022730.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(15436..15843)
                     /locus_tag="ACG80L_000444"
     CDS             complement(15436..15843)
                     /locus_tag="ACG80L_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS5022731.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(16109..17584)
                     /locus_tag="ACG80L_000445"
     CDS             complement(16109..17584)
                     /locus_tag="ACG80L_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS5022732.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17574..18422)
                     /locus_tag="ACG80L_000446"
     CDS             complement(17574..18422)
                     /locus_tag="ACG80L_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS5022733.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18459..18821)
                     /locus_tag="ACG80L_000447"
     CDS             complement(18459..18821)
                     /locus_tag="ACG80L_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022734.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18822..18980)
                     /locus_tag="ACG80L_000448"
     CDS             complement(18822..18980)
                     /locus_tag="ACG80L_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022735.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(19121..19745)
                     /locus_tag="ACG80L_000449"
                     /pseudo
     CDS             complement(19121..19745)
                     /locus_tag="ACG80L_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19742..20053)
                     /locus_tag="ACG80L_000450"
     CDS             complement(19742..20053)
                     /locus_tag="ACG80L_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022736.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(20097..20390)
                     /locus_tag="ACG80L_000451"
     CDS             complement(20097..20390)
                     /locus_tag="ACG80L_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022737.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20584..21231
                     /locus_tag="ACG80L_000452"
     CDS             20584..21231
                     /locus_tag="ACG80L_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5022738.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            21292..22437
                     /locus_tag="ACG80L_000453"
     CDS             21292..22437
                     /locus_tag="ACG80L_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5022739.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22518..22823)
                     /locus_tag="ACG80L_000454"
     CDS             complement(22518..22823)
                     /locus_tag="ACG80L_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS5022740.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22848..23279)
                     /gene="ruvX"
                     /locus_tag="ACG80L_000455"
     CDS             complement(22848..23279)
                     /gene="ruvX"
                     /locus_tag="ACG80L_000455"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS5022741.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(23276..23542)
                     /locus_tag="ACG80L_000456"
     CDS             complement(23276..23542)
                     /locus_tag="ACG80L_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS5022742.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23797..24522)
                     /locus_tag="ACG80L_000457"
     CDS             complement(23797..24522)
                     /locus_tag="ACG80L_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5022743.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24883..24953)
                     /locus_tag="ACG80L_000458"
     tRNA            complement(24883..24953)
                     /locus_tag="ACG80L_000458"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24919..24921),aa:Gly,seq:ccc)
     gene            25070..25822
                     /locus_tag="ACG80L_000459"
     CDS             25070..25822
                     /locus_tag="ACG80L_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5022744.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25838..26317
                     /locus_tag="ACG80L_000460"
     CDS             25838..26317
                     /locus_tag="ACG80L_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5022745.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26568..28148)
                     /locus_tag="ACG80L_000461"
     CDS             complement(26568..28148)
                     /locus_tag="ACG80L_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS5022746.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(28141..28884)
                     /locus_tag="ACG80L_000462"
     CDS             complement(28141..28884)
                     /locus_tag="ACG80L_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022747.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(29060..29836)
                     /locus_tag="ACG80L_000463"
     CDS             complement(29060..29836)
                     /locus_tag="ACG80L_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5022748.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(30084..30920)
                     /locus_tag="ACG80L_000464"
     CDS             complement(30084..30920)
                     /locus_tag="ACG80L_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS5022749.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(30931..31860)
                     /locus_tag="ACG80L_000465"
     CDS             complement(30931..31860)
                     /locus_tag="ACG80L_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS5022750.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            32052..32405
                     /locus_tag="ACG80L_000466"
     CDS             32052..32405
                     /locus_tag="ACG80L_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5022751.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(32479..33135)
                     /locus_tag="ACG80L_000467"
     CDS             complement(32479..33135)
                     /locus_tag="ACG80L_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5022752.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            33361..35295
                     /locus_tag="ACG80L_000468"
     CDS             33361..35295
                     /locus_tag="ACG80L_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5022753.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(35381..36604)
                     /locus_tag="ACG80L_000469"
     CDS             complement(35381..36604)
                     /locus_tag="ACG80L_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5022754.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(36598..38469)
                     /locus_tag="ACG80L_000470"
     CDS             complement(36598..38469)
                     /locus_tag="ACG80L_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS5022755.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(38490..39608)
                     /locus_tag="ACG80L_000471"
     CDS             complement(38490..39608)
                     /locus_tag="ACG80L_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5022756.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(39691..40524)
                     /locus_tag="ACG80L_000472"
     CDS             complement(39691..40524)
                     /locus_tag="ACG80L_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5022757.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(40517..41434)
                     /locus_tag="ACG80L_000473"
     CDS             complement(40517..41434)
                     /locus_tag="ACG80L_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5022758.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(41457..41636)
                     /locus_tag="ACG80L_000474"
     CDS             complement(41457..41636)
                     /locus_tag="ACG80L_000474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022759.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(41661..42785)
                     /locus_tag="ACG80L_000475"
     CDS             complement(41661..42785)
                     /locus_tag="ACG80L_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5022760.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        12829 a         8665 c         7127 g        14211 t
ORIGIN      
        1 aaattattct attagtgaca tccttgaact ataaaaatag gagtacggca tcatctgccg
       61 cactcctatt tttttcgtca tttatttctt aaataaaacg attctcacgc aattttattt
      121 ttttgatatc ttctactgtt tgtgttccag ctaattgcat gaccatttcc aattctgttt
      181 taaagaaatc aaacacttga tggactcctg tacttcctcc taaagacaat ccataaataa
      241 cgggtctgcc aatagcaaca agatcggcac ctgatgcgat tgctttgaaa acatgttgtc
      301 cacgtcgtac accgctgtca aacacgattg gtacacgttt atctactgct tctgccacat
      361 attgcaacga atcaaacgca gcaggccccc catctaattg acggccacca tggttagata
      421 cccaaattcc accagcacca gattctaatg atcgatagac atcttcttct gattgaacac
      481 ctttcacgta gactggcaaa tcagaatgag cagcgataaa ttctacatct tttggactaa
      541 gtttttgttt ggaagattta tatacagcat ccatcgtttg gcctactcct gattgataag
      601 cctgaacgat cggcattggc agcgggaagg taaacccgtt tctgcggtct gtttcacggt
      661 ttccgccaac tgttgcatcc gctgtcaata cgattgcttt tgctccatta cgcttggcca
      721 cttcaagaat atccaaattg attccattat ctttgctcat ataaaattga aaccattgcg
      781 gtgctttctc cccgcctgct tctcttatct cttctaaagt acaagaagcg taagaacttg
      841 ctgtatagat cgtcccaaaa tctgctacac cttttgcaga agctttctcc gccttcacat
      901 gagctaatcc atgagcagca actggtgcca tgatgattgg tgcagtcagc atttcttcgt
      961 caaaatgtgt agttgtatcg ggtaattcta catctcttaa tacatgagga ataatcaaac
     1021 gatgattgaa tgcccgttca ttttcttgat aagtaaataa gtcgccagca ccactgctga
     1081 tgtaaccata accaccagct ggaatcactt gtgaagccgc gatttctaaa tcttccatat
     1141 tgataaaatc tactattcct tcatttgtac ctgcttgata tgttttttcc atctcaatgc
     1201 cttctctcta taaaatttga atgatgaaca atatgatagc gtttacactt tttatattac
     1261 tcttcgacat tgatacaggc aaataatctg tttacatttc agccattcca gatgtcacta
     1321 ctctggaaga aatgattgta ataaaaatag ataaaaaacc gatgggactt tgacaaaagt
     1381 agtgtcaaag tccctttttt tgaataaacg gtgttcaaaa gccattttct cggtattaag
     1441 tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac tactcgaaac
     1501 taagcgactt tttcacaacc tctcgataaa acggtgttca atcagctgac cctcggtatt
     1561 agctcaaaaa atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg
     1621 tcagaacgtt gacttcacag tctcttcctt aaacggtgtt caaaaacttg catctgcggt
     1681 aataagccca accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact
     1741 tgatgcaggg caggtttttc acgacctctg attcacggtg tttctttttc atcaacattt
     1801 ttacgaaata ctgtggaaga ctattgaaat aagtgtggat tgtttctttg tgtttcaatc
     1861 gtttcttgcc attcttcatt taattttgga ttaccgtttt cttccttgaa ctcataaggt
     1921 cctaattcaa aaatcccaga cagtatttga ttcacgattt catatagttc agtgtgatca
     1981 gacacatgat tcttgaagat catctcatta tcaccacgtt tgaacctcac attcgggctc
     2041 tcatgatgaa cctctatgca tttttctcct ttttttaact gacggacttc tgaaaacatc
     2101 tcataaatat cttctccagt aaaaccttca gcaagcgcca gactgtccac aagaccgcct
     2161 actgtcagtt cagagattat tgcttgattg gaatctaatt ttaatgtata gcttgtcgct
     2221 tctactacgc cacatgcatt gcaaccgcct tctgatagtt cgctttttct aatcaattga
     2281 acttctttca ttttctcttc ctcctcttaa ttttctactt atgtattttt atcacctgac
     2341 ataggtgact cctatcaacg ctatcatgtg cttttttcct cagaaatcct atttatccat
     2401 tccatctatc ccttattctt ctacttttct ttctctagca tataagagat acgcatagta
     2461 ttcaagcaag gattgtcgat ataaaaagaa caaatactgc tattttgatt tcttttggct
     2521 atattacata taaataaaat ttatcatacc tgctcttttt gactatatca tcatctgact
     2581 tctatacttc gtttgtatta aaacttagga ggaatcattc atgcttacag ggttaaactt
     2641 attaaataat ccttttctaa ataaaggaac agcttttaca aaagaagaac gggcaaaata
     2701 tggaatcact ggtatgcttc caagtactgt ccaaacactg gaacaacaat ctgttcaagc
     2761 ttatggtcaa tacttaagca aacaaacaga tttggaaaaa cgtatcttct taatgaatct
     2821 attcaatacg aatcggacat tgttttataa attgatgggc gaacatttag ttgaattcat
     2881 gcctgtcgtt tatgatcctg tcgttgcgga ttcaatcgaa caatacaacg aaattttctt
     2941 gaaaccgcaa gatgctgcat ttctttcaat cgacgaacca gaaaacatca aagcaagttt
     3001 gaagaacgcg gcagatggac gagacattcg tttgattgtt gtgactgatg cagaaggaat
     3061 cttaggtatg ggcgactggg gtgtgaacgg tgttgacatc gctatcggca aattgatggt
     3121 ctatactgct gctgctggta ttaatccagc acaagttttg cctgtatcga tcgacgctgg
     3181 tactaataat gaagaattat tgaataatcc cttatactta ggcaatcgtc atgctcgtgt
     3241 agaaggcgaa acttactatg aattcatcga tcaattcgtt caatcagcta ccgaattatt
     3301 cccagaattg ttattgcatt gggaagattt tggacgagga aatgctgcag cgatcttaga
     3361 aaaatacgaa gatcaaatca ctactttcaa cgacgatatc caaggtacag gaattgttgt
     3421 cctagcaggt gtattaggcg gattgaatat atccggtgaa caattgaaag atcaaataat
     3481 tttgacattt ggtgctggta ctgctggtgt gggtatcgca aacatccttt tagatgagat
     3541 gatccgtcaa ggtgtacctg aagaagaagc acgccaacat ttctatcaag tagataaaca
     3601 aggtttattg tttgaagata cagaaggatt gactcctggt caaatcccat tcgctcgcaa
     3661 acgctcagat ttcactaatg ctgatgaatt gacaaactta gaagcagttg taaaagcgat
     3721 ccatccgaca atcatgattg gtacttctac ccaacctgga gcattcactg aagaaatcgt
     3781 gaaagaaatg gctgctcata caccacgtcc gatcatcatg cctttgtcta atccaacaaa
     3841 attggcagaa gcaaaagcaa aagatttaat agaatggacg gacggaaaag cattagttgg
     3901 aacaggtatc cctgctgctg atgttgaata taatggtgta acttaccaaa tcggtcaagc
     3961 aaataatgct ttgatgtacc caggtttagg acttggtttg attgcttcaa cagctacacg
     4021 agtaaatgct gaaatcattt ctcaagctag tcgagcatta ggcggaatcg ttgatgtaac
     4081 aaaaccagga gcagctatct tgcctccagt agcaaaaatc actgaatttt ctcaaacgat
     4141 tgctgaaact gtcgcaaaat cagttgtcgc acaaaacttg aatcgtgaag aaatcacgga
     4201 tatcaaagaa gcagttgaat ctgctaaatg ggttcctgaa tataaatctt tagaagatta
     4261 agatcgttat agtaaaagaa catacagaaa ctttgtatga cgtctaaaaa gaaaaaagcg
     4321 actgcggcaa aacagcggcc agccaactcc tgctcttttg tcgcttttct ttggcttttt
     4381 tcatatgata cagaataaga aaggatctgt aaaaatggca caaaaaacaa caactgcaac
     4441 tgcagttgag gaaaagaaaa aagaaggttt ttggtccaca aaaatcaccg gcgtaagttt
     4501 gccactttat ttgatcatgg tccttgtcct gatcattgca atggctttag ggaaattacc
     4561 gacgaatatg attggaccaa tcgcagtttt ggttattttc ggtaatttat ttcattttat
     4621 cgggacaaaa atccctatcg tcaaaagtta tttgggtggt ggttccgttt ttgcgatctt
     4681 cgcttctgct gcgattgcta catttgggat cttacctgaa tatgtagtga aatccacaga
     4741 aaactttgtc agcaatatgg gcttttggga cttttatatt gctgccttga ttactggtag
     4801 catccttggt atgaaccgta atctattgat gaaagcttct gttcgtttta ttcccgttgc
     4861 acttatttca atggtcgtct ccttttttgc cgtaggactc gtgggcatgc ttatcggaaa
     4921 tggttttgca aattccgttt tatacatctc attacctgcg atggctggtg gtgttggagc
     4981 cggagccgtt cctttatcaa cgatttatgc aaatgtttta ggtacggatg cttcttctat
     5041 catttcccgc ctgattcctg cgacagcaat gacaaatgtc ttagctatca tcggtgcagc
     5101 attagttgct cgtgctggac aatcaatgcc aaaattcaac ggcaatggta aattaatgga
     5161 aaaaggcgat cttggcgatg gaaaacctcg aactgtgaaa ccttccattc cacagttagg
     5221 tgttggtttg atggtatcat tgactttctt catcttagga caaattggta actattttgt
     5281 tcctaaagtc catgcttacg catttatgat tattatcgtt gtcatctgta aaatcgccaa
     5341 cctattacca gagtattatg aagatgcagc tatcatgttc aataacttga ttgtcaaaaa
     5401 cttgacagca gctgttcttg ccggtatcgg tattgcgttg ctgaatctga atgtattagc
     5461 ttctgctttg acatggcaat ttgttgtctt atgtctgaca agtgttatcg cgatttcagt
     5521 tgtttcagga tttgtcggtc gtttgtttgg tttatatcca attgaatcaa ctattactgc
     5581 tggcttatgc aacaactcga tgggcggaac aggaaatgta gccgtcttat ctgcagctaa
     5641 tcggatggaa cttattgctt ttgctcaaat gggcaatcga cttggtggtg ccatcgtttt
     5701 gatcatctct ggtttcttga tgcaattact ttcttagtca aaaaaacaaa aatacctgat
     5761 catcaagtat cggcttggtg atcgggtatt tttttgataa agatttacag gattttcaaa
     5821 aatctttttt gcattttatc ggaatgataa ccgatgtgtt cataaaattg gtgtgcttct
     5881 actctgtatt ctgcagaatt aagccttatt gtcgttattc ctcgtttttt cgcttcttct
     5941 tctacgccag tcatcaaagc ctttccgatg cctctttttt cagcatattt agacacagca
     6001 agtgccataa tattgaacat tggatcgcta aaaatcgatt cataggactc cgcttgtaca
     6061 tagccagctg cttttttaga agtaggatct tcataaacca agaaaaaatt tttctctggc
     6121 tccttcagca gtttcttcat ttgtctcacc gtaacggtta atggtatatc ataccctaat
     6181 tgatccgcat tgatttcacg tagtgcttct gcatcttcta aacgcatttc ccgtaacatc
     6241 caaatcacca gccaatttct gattttttaa aatgaatttt tttatcatag tatactcttt
     6301 ttttcattta gaacaatatg tttcagtgat tttgacttgc gttagtctag attctcctaa
     6361 tgtttgtaag atgacaaagg cattgtaaca attgaccaaa agaaccacga ggacaattac
     6421 ttgaaaaata aaggtcactt tttttggaga caaaaccctg ctaaatcttg ctccccacaa
     6481 acctccaata acagcagcgg ggataacata aaacaacata gataaatcaa agcgcataaa
     6541 gctacttgtt aaagcaatcg ttaccaattt tgccagttga gaaaagaaaa tcgtagatag
     6601 agaatatagt gttgcttctt ttattggcaa tgcaaacatc agcatcaaca aagaaacatt
     6661 gactggtcca ccaccaatac ctaaaaaact agctaaaaag cccagaaccc cccccacata
     6721 taaggtacca agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga
     6781 acgcaaaaac caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt
     6841 tttcaaataa ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag
     6901 ctccactaga tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc
     6961 gcacagtcga aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg
     7021 caacagaatg ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc
     7081 cggaaatagc gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca
     7141 tcttgtattt cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta
     7201 tacagtaaaa gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg
     7261 ctaaacgaaa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca
     7321 aaaatagctc ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga
     7381 acaccgttta agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt
     7441 tcaaaaaaca gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttggcagg
     7501 agaacaccgt ttaaggaaga gactgtgaag tcaacgtttt gacctgatta ttagagaaac
     7561 aaatggcaaa atagttcttc atatttcgcc gtttttcgag cgaatgccga ggggcagctg
     7621 attgaacatc gtttatttgg aaaaagggat tgtgacaaga cgtttgtcac aatccctttt
     7681 tttatatctt attaattcat tcttttgttc tattcttctt cttccgccat gaatgagaaa
     7741 cccatatcaa agaactataa aggttatcaa aacagcgttt atgactaaat gaattagagt
     7801 tatctatcac tatttttcaa gcgatttaaa ccaaatggca tacaatatgg catacaaaaa
     7861 cttcaataag ccgtgggggt ttttaaattt cttcttgttc tttaaaatca atgagtggag
     7921 ctacataatt tgaatccact ttttcaaacg tcactttttg catcgacgaa tgttttcttt
     7981 cccaagataa cgtaaataga tttgactctg gacttataaa gtctacttca ccttctagta
     8041 ttactttctt atgcaaatat tcctctaata aatatagact tgctaaagca tacaacacat
     8101 tttccaaatt tgcttcctta taatttgaag aacgaccatg ttttactctg ttatatattt
     8161 tccaccaatc tggtgtatct tcaggccacc catctccaaa aggttttagt gaagtcaatg
     8221 ttaaagaatt cataacccta acaccattat ccaaacacat taattcgcta gatttaataa
     8281 cagctttaaa atcagttatg cttttattgc tactgctatc aaatccgcaa agttctttga
     8341 aaataatctc acactccgaa ccaatcgcta gtagtagttt ttgatattca atagagaatg
     8401 ttttgaaatt attctcatcg agcatcacaa aatctgtggt attaataaac tcattttcta
     8461 aatgtagata atacttccaa taaaaatttg taaactctgc tttgtccatt aaatcacctc
     8521 ttttgaatta taacaaaaaa aacacgtcca caacagacgt gttctttgat ttctacacaa
     8581 tcagcttgcc aaaaccatcc aatcagtcct agcatgcctg tattaaaatt gaacgttcat
     8641 catctatcat ctttcttagt ccagcatgtt tgcaaccaca cctacaattt tcagtccaac
     8701 aatagatttc gactagctaa gtcttgcaaa attcaggcag ctaacccttc atttactacc
     8761 ttaattatac taaataccca caacgtttac aagtgtaatc attctaagaa atctgtttag
     8821 ctatgatcgt tgtataatct ttaaacgccg ttccttcatt gattgaaata attttataac
     8881 tcttgttttt ccaagctact ttcatatcgt ttgttagttc ataatcttga tcgtatcgaa
     8941 taataaacgt caacgttcct tcaagaatgg tacctacact agcaagcacg tcttttagca
     9001 tttgtgaacg cacattagcc caacattcaa atacagtttc ttcactatcc accatctgcc
     9061 catcttcatc ttttacccgt tttgtctttt taaacttgac tctctcagtc aattcattta
     9121 cattcttaat gattggcata ataagcacct ctcatttgtt gtactaatgc ttgtaccgtt
     9181 actggtatcc gttcgctgct ggcttcttga cggttcaaat accaatgttg agtaagtaat
     9241 gacaccgcca aatcaaattg ctgataatta tccatatctt tatccgttgc gctactatca
     9301 atagcacttt ttatatattc gcctgctgta tcaatcaatt gttgaataaa gtcatcatca
     9361 atagtgtgat cgatccgcat actttttttt atgctatcca aaattaccat tttatctctc
     9421 ctaatgaaaa aaaggcagtg ggataaaccg ccgcccaata tttttctata cttttggtgt
     9481 tacaactggc gtaaactcaa tatacactga agcattttga tcaattttct tataatcgtt
     9541 gcgaacgatc actgcaagtc cttgtgagta gtaatcgaat ttttcccatt gagtcgttac
     9601 ttgatttcta cgagcaacaa agatagattg tgcaatatcc cccataatca tagggaatgt
     9661 tccagcttta ggattggcaa acaatgtatc tgcaatcaat actactggca taccgaataa
     9721 tgatttacca ctaggtgctg tcacatcggg ttgtaaaatg taacgtccat ctgaatcttt
     9781 caatgtatct aggtggtaat atccgctttg gtttagaatt accattttat ttaatgctgg
     9841 gtctaatgcc acattgtata gttgtttcaa atcgtccaac gtagcagccg ttttcttagt
     9901 gaatgttttt aataaatcca tgatatgctt attgtcagtg ttttctacca atttcgctaa
     9961 ctgatctttg acctcttgta caatattcac tgctgaatct tccacaacct catttgataa
    10021 ggcaatctta ccagcacgag tttctacttt ataatcaact gaagtaaaca tttcagcgtc
    10081 aatatcacca atttcagcaa gttcagcttt tgttgctaac actgcttgtt ggttagttgc
    10141 tactggatat ttaccttgtc catttgatac tgtttttact gttgcatatt gagctaagtt
    10201 ataatttgaa cgtttcaaat caaagacttc accgattact tcttcgggaa caactgccgc
    10261 tgcatttaca gtagtgactc catcacgcac ttcgccttgt gaacggatat attcttcata
    10321 cccacgcaat tctgtttttt cgttatcgat taaagttttt tccatgttat tactctcctt
    10381 ttgtgcttta gaagtggatt ctaagctatt gtttgtttgt tttgcttggt tgttactcat
    10441 aaacgattcg taagaacgct tatttacttg gacatttgat gaatcataag cgggaacggt
    10501 cacgacacta atttcattaa gtgctttgat tttatctatt gatcgttcaa ttgtgccatc
    10561 ttcttttttg tcgaatgaat ctaatccaag cacgaaccca aaggacatag aatcaacaac
    10621 cccttttgag atattctcat acacatcatt tgaataggtc gtgtcggtta gctctgcttc
    10681 aaaatgtaat cctgtatcat cgacattcaa tttcaacgta cctgctttga cactagctag
    10741 tggctttgaa taatcgtgtc catataataa gaacacattt gataaatcca cctcttttaa
    10801 tgcttcgggt gtaatcactt ctacaaagcc gcctaaatct tttgacggtt gcccgaattt
    10861 caacgcatag cctgaaatag ttttgccgtc tgttggttgt ttttcatttt ctttctctac
    10921 tttgggagaa tcggctgtta gctcagcttc ttctgtcagc cgtttttcct tttcttcttc
    10981 attattcaac tggtgtaact ccttcctgtt cttttaattg atatttctcc aacgtatcta
    11041 aatacgtaaa gttcaaactt gctagtaaac gatcgccacc atccatgggg ggtagcccca
    11101 ttttcgaccg cccctcatta atcgtgagaa gtgacccttt gacctgatcc aatacatttt
    11161 ttacttttgt ttcaggatca gtctctagta actgatcgga attaaagcgg atattggttt
    11221 caagtttcgt atctaattca ctcacaaaca cattaaagta atgaatcagc gtactttgga
    11281 tatactggag attactttga acggtgctag aatgttcatt ttcaacgcct aaacgctcaa
    11341 tcggtacacc aaacgccttg gcaatttgtt ttgtgttcca atcattggaa ttgataagct
    11401 tcaaaacatc ggtgtttatt tctaatgttt tgtagtccat tgtttcatca agaatgatcg
    11461 ttctaagagc attatctcca ctacttgaac cgttggcttc ttcaaacttt tcacgaattg
    11521 cgttctttgc tgatccgtct aaatctgact tatgaacctt caaaatacct gacccactga
    11581 caccacgagt aaagaaattg tgcaatgtgc ggttgccagc ttgttgaatt ttcagttcat
    11641 cacgtaacgc ataaagtggt ggtaatcctg ttaaaccatc ttgggtgaaa tatttgaagt
    11701 gcaaaatatc gctagacttc acacgtcttt ttttatcacc aatctgataa gacaacgtac
    11761 cattatctaa ttgagtaacc gttacacttg agttaggtaa taagtgaatt tcttctacct
    11821 tttcaccgtt gcgtttaatc tctgcaaagc tattaccgtt tagcaacata ttgaccgcca
    11881 aagcaaactt taaatgccaa ccatccattt ctgaattagg tttctcgttt agtaacttca
    11941 ccagttcatt atcagcttgt ggcatattgt ttttgaccaa ttgaatcgga cttgaagcaa
    12001 tatcactagc aataattcga acggctgcaa acacatcact atttcttaat gcacgaacgc
    12061 ttgtaaagct ggtggtgtaa tcatccgatt ggattgatac cacatgttgt aggaaaggat
    12121 ctcccgttgt attttgtctc gctttaaaaa atgccatctt ttaacctccc ttctgttctg
    12181 tatttagcat gaccgctacc ccaataagca ctatgcccag cgctaacaag ccatagtaga
    12241 catttgttag taaggtgata gcaaccaaca cacacaccaa tccaagaagt aaaagaacgg
    12301 tatgaatgta attagaaaga aaattcttca cttgtataaa attcattgtc tgctttcgct
    12361 ccttccgttg tggtgaagta atccattgca aacacatagg cattgattaa tgcagctatt
    12421 ggatcaattt tgttactgtt ctttgattta ttaatctgaa tgccgttgtt atccgttttt
    12481 aaaatagcgt tgttcactgc atgggtgaga atcgtatttt tattgtgaat aacgttgcct
    12541 tcataaactt gttctctaaa agttcgggtc ggaacgttta gagtaatcgt tccttgtctt
    12601 acttctaaca ttgggtagtt ggctttttca gctttactga taagcgaatt agcgttatac
    12661 gggtcataac aaatccccat acattccaaa tcattttctt gaatcaaatc ttgaataaac
    12721 tgaaatactt ggtcatagtc cacgattcca ctttctaatt gagtaatgga acactcacca
    12781 gctttttcta gttctcgata aggcaaaccg tcgcgctttt ctttgtcttg aagcccatat
    12841 ttggtggcta caaagctatg tgaatcacaa taaagttcgc cgttatctag cggtacgatc
    12901 catgaaacgc tagttaaatc atctgtttta gataaatcca ctccgatata aacgggcttg
    12961 cctgtaatat cttgtggttc tacttcggtt gcctgccagt cttcagcaat catatagctg
    13021 tcctcactcg cttgtctcca taaattgaag tttttcacca atacagaatt aaggttattt
    13081 tgtttcaaag caaggttcac atcatcttga atggttggaa tcattacttt tttgatttct
    13141 tcactttcaa aaatcggatt ggctttgatc catttctctt gatcgtgaat ttcttcggga
    13201 tcgtccaatt cccatatcgc tataaagtac ctgtcggctt gttcttcgcc ttttagcaca
    13261 cgatctaaca ataagtattc ctcgtacata ggaacgttta aatcaagccc agcggtacta
    13321 ataacacaca ataagccgtt cttttgttgg gtcattcctg acttaatgac gttgtacgtt
    13381 ttacgagttt ttgcttcgtg ccactcgtca agaattgcgg ttgttggtgc aaacccatct
    13441 agcgtgctgg tttcactagc cagtgccata gcgaacgaat tagagggcaa gtgctgaatc
    13501 ttagagttca taattttcag ttgtggtctt aaaaacttac tagacttgac cacgtttcta
    13561 agtgaattag acagcatatc atagcccaat ttagcttgtt tcaaagcgtt acttacaaac
    13621 aatacttgcc ttgcttctgc tggttcttgt tccataatca acgcattggc agccatgcca
    13681 cttgcgatat acgttttccc attcttacgg ctcatactgg taaacgctcg attgaatcgc
    13741 ctgtaattcc cttctttagt acgccagcca taaagactgc ctagaatgaa tttttgaaat
    13801 cctaacatct tgattgcttt accgtccgta gatggtagca actcaatgaa tttaatcgct
    13861 ttggtggctt gttcttcatc gaacacataa gggaagctat ctgacttaga acgctccaaa
    13921 tcgcttatat gacgctcaca cgcttggata attttcttgg gtgcaatcag cttacctgat
    13981 aggactttct caatgtaatc attcatgaat tcatcaactc tttgaacggg tcttcgggtt
    14041 catcattttt aaccttgttc aaagctagtt ttgatcgtga ttctaaggtc atacctagtg
    14101 aggtggcttg ttttttcaaa tcactcatag cttgtgattg aacccgaacc gctggattgg
    14161 ctttctttac tccgttttcg catgtgataa acgtacctga ttttcttatc tcggcttcgg
    14221 ctgttttcat tcgagcaaag gctaaacaat aggctaccaa tgaaccataa tctgcttcac
    14281 tcaatggata atcttttttt aatgttggta cgatacgatt ccattcactt ctagcgctcg
    14341 ctgccatcca gtcagggggc gtgatattta ttaatggttg ctgcttgaac aattcttctc
    14401 tagcgtccac acggattgct ttttcttcac ttgatatatt gccttttgta tcgtgtaata
    14461 ttttagggtg tcccatgttg tcctcctttc agacaaaaga aaaaggaacg taagcaatta
    14521 agctcacatt ccttcgtttt tcgatcagaa tttttcttta tctaatttat ctatataaat
    14581 gatttttccg ttctgcattt gaatggtcac ttgaccaaaa tgaggtaact caatggtgcg
    14641 ctgtttccct gttttatcag taataacaat tgtatttttc aataaatctc ctactttcat
    14701 tatatacttt ttcacttgtg attacaagtg taatcgttat ttgtaattac ccttatgcct
    14761 tttcacaacg gttatttgta ccaaaatgag taggcatcga ttgtcagtaa gtttcacact
    14821 atgccccctt aactcaaagg gtactctttt ctcgtctttg tctttcattg gctgtcttgg
    14881 aattatggtg tgcatggcat aaagaacgaa aattagtttc atctaaccgt ctattccaat
    14941 catcttttag ttcaattttg tgatctacaa catcagcttt ccttatcaag ccttcttcta
    15001 ggcaatcctc acatactgga tgatttaacc gatacaagta tgaagccttt cgccatgttc
    15061 tactttgata gaattgaaaa tatttacctt cgctttgctt tctttcttca cgcttaattg
    15121 ccgtttgctt tgctttatgc ttcccacaat acttctgcct ataatcaatc aatgttttac
    15181 atccagcatg attacattgt tttttaactg ccatagatta ccaaccaaag cgatctcgtt
    15241 cgtcagaatc atcttgttcc cattttgatt tgtattcaat ataaggcgaa actatcttca
    15301 agcacgttcc gtctttaaaa ttaactttag tgattttatc ttcctcaaaa ctaataaagt
    15361 ctacatcttc ttgagtgttt gaatactcaa tgtgtttctc accatcgaag tatctaatac
    15421 tttccatatg cataattatt ctacctccaa taaattagtt atcaattcat catctgttag
    15481 tagcaaagcc aagaacttat catggatgtt taaaaacaaa ttatcctcat tcgatgtaag
    15541 ctctcgttgt ctcgctcgat catataggtt aagcagacaa ttaaattcat ccattagctc
    15601 acctgtatca tcatagttgc cactatctac caatgatccc actactagcg attgacacca
    15661 cgctactgac tcctgcagcc aatcaggttc ttcttccatt atctttaata cttctggatc
    15721 aaatgtggaa taatcttctg ttaccaatcc cacactaaat gccagcgtgt tcattagaaa
    15781 ccgatccatt tcatctgtat attgaaagtt cggtgctttg tttgttgaaa taatattgag
    15841 catttacttt cctccttgat tcgtaacacg tggtaacacc tacgtaacag gtacccgtta
    15901 ccactcaatc ccaacgggga caagggttct agcgttttgg taacaggtaa cgggtaaaat
    15961 ccagtaacat tattccccag catattagct aactatttca aaagttttaa ggaaataagg
    16021 tgttaccccg ttaccaaatt gctgtatccc ttggggcagt aagggtaaac tagtaacggg
    16081 ctaggtgtta ctgacccgtt actacccgtt actttttgta ctttccagct cgatttttat
    16141 tttcataggc atttgactta ttgtaaaatc ctcgtcgttt atttcttttt ggcagttgct
    16201 tccgttccca accaggaata ctgctaataa tcaaggcaat ctttttagtt tctgcatctg
    16261 atcgaccatt caataaatcc tttgcttgtt tatcaaaaac agcttccaaa atatcagcgg
    16321 tgagtacgct atcaactaaa taaaactctt gttgcatagt agctccatta tagaaatgct
    16381 gtaaattttt ctcattacat ttactgtcag gatagttctg ataataagaa cgtctcgccc
    16441 acgctggagc ttcttcccat ttatacggga tttccatttc caaatattta gtgattgctt
    16501 ctttaattgg attctccatc ttggcatcct cttgatagtc ttttgcaatt tctaagactt
    16561 ctttatcttc atcgggacta aaatagattc gttgtccttt atcaaaaagc acctttgctt
    16621 cagctaacac ttgaagaaaa tattcatcct ttgttttaaa cggattcatc tttggcttat
    16681 tcttacaagg caaaggataa aaacggcggt ttcctgtatc atctttcaaa tactgcccat
    16741 cattcgacgt tccaataaat acacattgcc ttgcccaagc tatgacattc cgaccataag
    16801 gaggtctaat tttatcgatc ttggcactga tgaagttttt tatctgttca attttagttt
    16861 tgttcattga agataattct ccaagttcaa taattacatt gccttgaagc tgcatataat
    16921 catctttctt ttcacctagc gaatctaatg aatcggtgaa aaaatcatcc gtgtaaagta
    16981 atgctggaac ggtactctta ccaatccctt gcggtccatc caaaactgga acgatctcaa
    17041 actttattcc tggtttatag atacgtgcga ctgatccgac tatccaacgt tttgttactt
    17101 ctcgtgtgta aaggttgtcg tctaccccta aaaggtcaat aaacagtgtt tccagtctag
    17161 gctgttgatc ccatttaact ttttcgatac gttcttttac tgggttgaaa acattctttc
    17221 tagccacatt tactactgct ttgtgtattt tgttgtcatc aaaaactaca ttgtagtttt
    17281 cttcaatata tgccgttaag gcatcctcaa aactgtcttc ccattctcca gcggtacgat
    17341 tgatccaagg agaatctttt aataggtgaa tgtatccgct aaattcgtta taagcaattg
    17401 ttccagctaa attgctatca ttggttaaaa tcaattctgc attcacaaca ctgttacttt
    17461 taatctgatc gtctttattg catcttaatc gtgacatcca ggttttcaat tcaatgatat
    17521 tgtcttcacg ctcgctggct tcgggaaagt ctaaaatact atctaattta tgcttatgag
    17581 ccatgagctt tccccctcaa atctttttta atcatcgatc gaataatttg attcacttct
    17641 ttatctttta gcggtggttc attgtacgca tttgctgtat aaatcaattc tgctaaagtt
    17701 tgattatcta caccgtgcca tagtaaccaa ccgcataact tagttaaatg atcgtttcta
    17761 ctaccttcgc cagcgccttg aacaatcata tctaattttt ttgctgtccc tgtttttgct
    17821 acgggtcggt attgagtctt tatttcttgc ggttttttta gcagtaggtt cactagccaa
    17881 tcgggagctg gtgcaatatt atttagagat acgttttcaa taggttcata tgctctccca
    17941 acacttcttg aaataatcat agttggcgct attggaactc ctttagtgat aatatcaatc
    18001 ccgcttaaac ttttaccttt atcatcacgc ccgaagatat cagcaaatgc acctgtttct
    18061 tgttggataa ttacaccatc aggaatttta aagaagaaat ggattccttt acctggtgtg
    18121 atttccgtat aagtagaagg aaatggatca tacattgaag caagtttttt ataattctca
    18181 acgccgtttc gtgtttcaga atgttgatct aggtcaacta ccaccatttg atgatcttca
    18241 agcattaacc caatatttct agttgcatct tccgtccacc attcattaat ttgattagaa
    18301 tcgttagttg catcattcca accactagag ccttttattg gaattttagt tccaggagat
    18361 aagggataaa ttgaccatcc cgacttttgt aattccgttg ctgcttgata attatttgcc
    18421 aataaacctc tcctcctttt tcaaaatact ggtataaatc attcagacac ctccatgagc
    18481 ttcaactgct caagttcgta tttcaaccca tcccgaacaa attcagcacc ttttaattga
    18541 ttctgatact ttacccatgt attaaatgtt ttcacccttg cttcttgcat atcagtaatg
    18601 tttaaccatg cggttaaaat ctgatttttt agctcgttga tttctcgaca aattattttg
    18661 tgttctgcat gttttttgag tacatacaaa actgttagtt tttcattgaa tgaatttcca
    18721 aagtaggttt tcccgatatt attttctagt tcagcgatcc cttgattaat ttcctctaat
    18781 tcaaggctat cgagttggtt taatagtttt gttttgtaca tctacttatg ccctccagtt
    18841 cttaattgat agtcgtactc gtcataagca gtgaagagat ataacgctaa tcctgcaaag
    18901 atagctattt ttgcaattaa tggtaataag ccgaaaattc cgccgattaa aatgcttcct
    18961 ataattaagt ttcgtttcat aatggttccc tccgattttt taatatggta taatcggaac
    19021 aaagaatatc gggttttatc ccaattaagt tgcctttatc ctgtccgcca agactgttat
    19081 ggataaggct ttttctttgt tctcgatcat gttaatgctc ctaagttgtc tgcaattcag
    19141 cagaacgatt ataaattttc tcactcactg aaaactgcaa cccatatttt tctttaacag
    19201 tcattaattc tactgtctca tctaaaatag gttcacgatc aaccaacatt tcaggagtca
    19261 tatctttctt ttttaccatt ttggaatgac cataaatagt tgatacagct ttattggcta
    19321 tcacattcgc tttaatgtag tctactggtt ttggttcagt aatagcgtgg cttaatttag
    19381 tcattgcttc tttttggtgt tctttgtcta gcatgcggaa tacttgaaag ccttcaagtc
    19441 ctgtggactg gcgtagttct ttgatgatgt cgaaaatcca gtcttgaaat gcctcggctt
    19501 cttttttacg gcttttgaat gccatgcggt aaatattttt tttcgttgat gatgtttaca
    19561 tcttgtgttc tgcccaatga atcgatgacc ttactagtag taaccccatc tttaggcaaa
    19621 gagtgaatgg ctctagtaac ctgtttaaga cctaacgctt ccgcaacatc tttcgccact
    19681 gcccaccatt catcattaat atcgacaaat cttataatgt gtccgttcca aatttcagtt
    19741 ttcattcaac gtcctccagt tcaagcccta gcaagctcgc taacaactca acttgctgat
    19801 acaaaatttc ttgatactct tcaatagaaa gtcgttcttt aaactctgct tgtactaaat
    19861 cgttcaagcc acgcaatact ccgcttaccg ttggttcttg tggtagcaat ttttgatagt
    19921 ccatttgttc gtacaaacat tcaaacgttt cttctagcgc tttgccttcc aagtttgcta
    19981 attctgccgc tgtgtcttct gtaagaattg ttgataatac tttttcgatt gctttatttt
    20041 gtgtcgattc cattattatt tcctcctatt aatccctttg cccccacggc ttgcgtttat
    20101 ttttggtatt cttccataaa gcgatcacat tcatcttgtg aaattcttac gactccatca
    20161 actataatca actttaaacc gttaggaaca tatttctgca tcatagtatt gtatgaaacg
    20221 ttcatatact tagcagcttg tgccttgttc atatattttg gaaactggta ctttttttct
    20281 aataatgaaa gtaacgttcc ttgagtttgc tctacttgtt gaacaactcg atcgtcaata
    20341 attttaaaag ctggatcatc aagcgtgagt ttgtcaattt ttaattccat atttatcccc
    20401 cctcgttagc atacatttct atacaaagat tttaaaaaaa tgcaagaggc tcttgaatac
    20461 atttctatac ctcttataaa ctcaatatac catcatacct tttcatttgc aagaatttta
    20521 acatactttt ttgaactttt atgtttcaca catatttaaa tcgtgatata gtagagaaaa
    20581 tatatgaatg gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt ggagacagga
    20641 caaagaatta gactggctag aattgaaaag ggactctcaa tggaacaact tggaggtaaa
    20701 ttatccccac cggccagtaa aggtgccgtt agtaattggg aaaatggcta taatcttccc
    20761 aataacacca ggcttaaaca actttcaaag gtattagatg tatccactac ctatctatta
    20821 aacggatctt atacattaag agatatgcat atgatgcccg aaattaagcg aaatgtgctt
    20881 gaaaagcaat tgaaaaaaac tattgaaaaa aataactcta taattgattc taatctttct
    20941 aatgctatca ataacatcga agaagataac ctgactgtct cacagaagat ttatttaaca
    21001 aacgcaataa aatatcttaa aacaggatct cgaattgaat ttcctgacgg gagtacccca
    21061 attgcttatc tcgcagctct tctaaatacg cttttacgat atgactcatt aattaatgat
    21121 tcagaaaaaa atacagatga gaaaaaagag tatcaagaat tttatgaaaa tgatcttcta
    21181 aaagagttca cagctcttgt tactgagcta aataaacact attcaaaata ataataaact
    21241 gcctcctcca accgccccca cggcttgcta cactatggag ggaaaactat tatggcaaca
    21301 ttcaagcaat atgaaactaa aaaaggtaaa ttttggctct atgaagccta tcttggaatg
    21361 aacaaaatga ctggcaaacc tgataaggtt cgtagacgag gttacaaaac aaaaaaagaa
    21421 gcccagcttg ccttgtcacg cttgcaagtg gactacgata aaaacggtat taaaaaagcg
    21481 aacaacgaaa cgtttaaaga agtttatgac ttgtggctag aaacatataa aaccactgtc
    21541 cgagaagcca ccttcatgaa aactgaaatc aagtttagaa agtggattct tccaaaatat
    21601 ggaaatcttc gtgtgaatga agtcacggta aaaaaagccc aacaaattgt aaatagctgg
    21661 gctgaaacta ctgaccaata caaggtactt cattcaactt ctaagcgtat ttttagctac
    21721 gctatcaatc ttgggattat tgagactaat ccactagaca acgtattaat gcctaaaaga
    21781 accaccacta agcataaaga aaaggtcaaa gtttacagta aagaacaact gatcaccttg
    21841 tttgattacc taaacagcaa acctgaaact tatcgaaacg actttgataa aacgttacta
    21901 cgctttctct tttatagcgg ggttaggatc agtgaagccc ttgcattgaa ttggtcagac
    21961 attgattttc aagaaaatac ggtcaccatc aataaaacac taagccaatc caaaaatggc
    22021 tataaaatca gtgatcctaa aactgattgt agtgctgggg ttcttccact agatgaaaga
    22081 acaatttcta tattaaaaaa atggcagatt aatcagcgga aatatatgct gacacttggt
    22141 gtaactgatc cgactatgat tttttgtggt atctataaag aactaatcac acaccacgct
    22201 atttatgcaa gattgattac tatttgtgaa aaagctatgg ttccatttct cggtgtgcat
    22261 gtagcaagac acactcatgc ttcaatgctc ttggacgctg gagctactat ggtagaagtt
    22321 caaactagat tacggcatac aaaaattagt cagacgatcg attgttacgg tcacttggca
    22381 aaagaaacga aagaaaagac tgttgaaaaa ttagtacaac acttaaatat tgactagatg
    22441 gcatacaata tggcatacag taaaaaataa aaaaaggaga aaccctatca tatcaaggtt
    22501 tctcctagct ttatagtcta ttcttcttct tccgccatga atgtattgaa tacttcttca
    22561 atcatgtccc attctgcgtc tgtttcgatt tgttctaact cgccttctgt tccatcttcg
    22621 ttttcgatgt atgcataagc ttgtaattct acatcttcat cttctgaaac accagcagga
    22681 tataaaagaa catagttacg tccaaattct tcttgtccgt caactgttag taggatttca
    22741 tataatgttt catttccttg gtcatccacc aatgtgatgt gttcgtgtcc ttcatggtcg
    22801 tgggaatggt tatgttctgt cataagatta cctcttttct tttattatta aaataattgc
    22861 ttacctgagc cgtctaaata attttgtaag atcatcacag ctgctaattt atcgatgact
    22921 ttttttcgtt tggctcttga agtatttgcc tgctctacta gcatacgttc tgcctgaaca
    22981 gtggttaatc gttcatcttg ataatcaact ggcaaggaga atagttcttt gatcttttct
    23041 ccgtatgaca tggaagcttc tgcccttggt ccgatggaat tgttcatatt tttaggtaat
    23101 ccaactacaa accggtctac ttcatattgt gctactaatt ccttcaaccg atcaaagcca
    23161 aactcttctt ctgcttcatt gatccgaatg atctctactc cttgagctgt ccagccaaag
    23221 gggtcactga tagcaacacc tactgtctta gaaccgacat ccaagcccat tactctcata
    23281 aattcactcc atgatttgag aggtagtcct tgacaattac ttccatgatc tcatcgcgtt
    23341 cgtgacggcg aatcaaattt cgagcatcac gataacgagg aatgtaggcc gggtctccag
    23401 aaagcaagta gccgacaatc tgattgattg gattgtagcc tttttcttcc aaagcccgat
    23461 aaacaatcgc taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta
    23521 cggtttcatc tgtaaaaccc atgattctac acctctttcc cgcaaggtaa ttccagctta
    23581 ccttctacaa ccttatttta ctcgaagaaa gggaaaacta caaacaaata ttccaaactt
    23641 ccgaataaat taagaaacac tatttgtatg tttttttcct tttttctgta gctttatcaa
    23701 aagtactctt ttcataaagt atacgctata tttctttgaa tgtatgtttt tacacaattt
    23761 tttcataata ttcataaaaa attcgaatag ctgctactaa ttttttgttt ttgttgcttg
    23821 tcctgtctca taattgatcg tgtagccatc ttgaacattg tagataaaga cattgaattc
    23881 aatttttttg tcttcaatgc tttctgcttc caattgcact cccctggcta ccagttcatt
    23941 cccctcaaaa tgaggagtga cacggtagcg tacatgatga tttgtttctt tgatatatgc
    24001 cgcaatatca ttttcgtgag caagcatgta tggagcattc aacgaacgtg tccctgtcat
    24061 caagttttta atgttgttat tttgaccagt cagctggaaa ccaattaaat ggctgcgatt
    24121 atataaccat tgcccgtcac ttaatttctt ttgtttccaa cctgtcgggt caatatacaa
    24181 aggctctctc ttttcagtag ggaacaaatc ttttccaagc atagcattgg cttgtcctac
    24241 tctgttcaat ttatcaatat ttgaatagct ttcccatgtt cctttggaaa ggctaagatc
    24301 ctcttttgta aattccggct gattcccatt gatttctatt tcttgatgtt ttccatcata
    24361 ttctggcaca tccgctactt gagaagcttg tggtgtggaa tcaaaaatag ttgtaatctg
    24421 ctgaaagata tctgaatctt tttgtgtggt ggaaacaccg aatgcccctg caatcacagc
    24481 agcgacgatc cccacgatcg ccttggttgt ttttttgctc atttctttac tcctttacta
    24541 acagctccct aaatcttaac atatgttccc ctataaataa atactagaaa gaaatagaaa
    24601 cagcaaaaaa taaaacctgt tttaagatgg cttaaaaata caaaaaagag acggattcct
    24661 cgtctctaat cagtcaccac tctatctgta aggtatttat tattattcaa aaattttctt
    24721 catactcaag acaatacact gatttcacaa ccgcttggta aacggtgcgc aaaagcttgc
    24781 atctgcggta ataagtccat ccaagttaaa aacataagaa gttgtttttg cttggctgtt
    24841 cttattactt gttgcagagc agctttttca caacctattt taagcgggtg acgggaatcg
    24901 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcaatacaa
    24961 aaacagtata cgcctctttt attccttcct caagtttttt tattctttac gcttctggta
    25021 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
    25081 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
    25141 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
    25201 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
    25261 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
    25321 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
    25381 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
    25441 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
    25501 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
    25561 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
    25621 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
    25681 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
    25741 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
    25801 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
    25861 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
    25921 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
    25981 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
    26041 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
    26101 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
    26161 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
    26221 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
    26281 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
    26341 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
    26401 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
    26461 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
    26521 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
    26581 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
    26641 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
    26701 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
    26761 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
    26821 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
    26881 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
    26941 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
    27001 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
    27061 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
    27121 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
    27181 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
    27241 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
    27301 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
    27361 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
    27421 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
    27481 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
    27541 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    27601 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    27661 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    27721 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    27781 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    27841 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    27901 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    27961 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    28021 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    28081 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    28141 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    28201 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    28261 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    28321 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    28381 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    28441 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    28501 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    28561 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    28621 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    28681 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    28741 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    28801 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    28861 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    28921 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    28981 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    29041 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    29101 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    29161 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    29221 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    29281 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    29341 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    29401 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    29461 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    29521 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    29581 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    29641 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    29701 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    29761 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    29821 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    29881 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    29941 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    30001 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    30061 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    30121 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    30181 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    30241 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    30301 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    30361 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    30421 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    30481 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    30541 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    30601 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    30661 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    30721 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    30781 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    30841 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    30901 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    30961 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    31021 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    31081 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    31141 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    31201 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    31261 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    31321 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    31381 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    31441 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    31501 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    31561 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    31621 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    31681 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    31741 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    31801 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    31861 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    31921 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    31981 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    32041 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    32101 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    32161 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    32221 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    32281 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    32341 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    32401 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    32461 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    32521 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    32581 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    32641 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    32701 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    32761 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    32821 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    32881 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    32941 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    33001 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    33061 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    33121 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    33181 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    33241 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    33301 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    33361 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    33421 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    33481 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    33541 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    33601 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    33661 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    33721 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    33781 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    33841 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    33901 caacaccctg acattttgat tctagatgaa ccaacgaacc atttagatat cgagacactt
    33961 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    34021 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    34081 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    34141 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    34201 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    34261 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    34321 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    34381 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    34441 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    34501 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    34561 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    34621 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    34681 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    34741 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    34801 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    34861 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    34921 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    34981 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    35041 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    35101 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    35161 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    35221 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    35281 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    35341 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    35401 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    35461 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    35521 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    35581 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    35641 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    35701 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    35761 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    35821 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    35881 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    35941 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    36001 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    36061 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    36121 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    36181 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    36241 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    36301 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    36361 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    36421 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    36481 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    36541 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    36601 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    36661 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    36721 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    36781 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    36841 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    36901 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    36961 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    37021 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    37081 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    37141 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    37201 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    37261 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    37321 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    37381 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    37441 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    37501 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    37561 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    37621 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    37681 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    37741 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    37801 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    37861 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    37921 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    37981 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    38041 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    38101 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    38161 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    38221 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    38281 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    38341 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    38401 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    38461 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    38521 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    38581 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    38641 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    38701 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    38761 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    38821 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    38881 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    38941 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    39001 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    39061 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    39121 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    39181 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    39241 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    39301 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    39361 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    39421 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    39481 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    39541 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    39601 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    39661 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    39721 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    39781 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    39841 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    39901 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    39961 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    40021 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    40081 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    40141 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    40201 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    40261 actggctgta gctaaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    40321 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    40381 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    40441 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    40501 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    40561 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    40621 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    40681 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    40741 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    40801 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    40861 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    40921 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    40981 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    41041 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    41101 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    41161 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    41221 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    41281 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    41341 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    41401 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    41461 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    41521 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    41581 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    41641 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    41701 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    41761 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    41821 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    41881 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    41941 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    42001 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    42061 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    42121 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    42181 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    42241 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    42301 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    42361 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    42421 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    42481 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    42541 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    42601 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    42661 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    42721 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    42781 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca ct
//
LOCUS       DAZUMB010000011        42096 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000011 DAZUMB010000000
VERSION     DAZUMB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.011"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..791
                     /locus_tag="ACG80L_000476"
     CDS             144..791
                     /locus_tag="ACG80L_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5022761.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            806..2332
                     /gene="zwf"
                     /locus_tag="ACG80L_000477"
     CDS             806..2332
                     /gene="zwf"
                     /locus_tag="ACG80L_000477"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS5022762.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2381..5179)
                     /gene="ileS"
                     /locus_tag="ACG80L_000478"
     CDS             complement(2381..5179)
                     /gene="ileS"
                     /locus_tag="ACG80L_000478"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS5022763.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5441..6148)
                     /locus_tag="ACG80L_000479"
     CDS             complement(5441..6148)
                     /locus_tag="ACG80L_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS5022764.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6169..6951)
                     /locus_tag="ACG80L_000480"
     CDS             complement(6169..6951)
                     /locus_tag="ACG80L_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS5022765.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7000..7221)
                     /locus_tag="ACG80L_000481"
     CDS             complement(7000..7221)
                     /locus_tag="ACG80L_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS5022766.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7302..7901)
                     /locus_tag="ACG80L_000482"
     CDS             complement(7302..7901)
                     /locus_tag="ACG80L_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS5022767.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7915..8592)
                     /locus_tag="ACG80L_000483"
     CDS             complement(7915..8592)
                     /locus_tag="ACG80L_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS5022768.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8609..9850)
                     /gene="ftsZ"
                     /locus_tag="ACG80L_000484"
     CDS             complement(8609..9850)
                     /gene="ftsZ"
                     /locus_tag="ACG80L_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS5022769.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9873..11198)
                     /gene="ftsA"
                     /locus_tag="ACG80L_000485"
     CDS             complement(9873..11198)
                     /gene="ftsA"
                     /locus_tag="ACG80L_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS5022770.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11354..12574)
                     /locus_tag="ACG80L_000486"
                     /pseudo
     CDS             complement(11354..12574)
                     /locus_tag="ACG80L_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
     gene            complement(12589..13677)
                     /gene="murG"
                     /locus_tag="ACG80L_000487"
     CDS             complement(12589..13677)
                     /gene="murG"
                     /locus_tag="ACG80L_000487"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS5022771.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13703..15064)
                     /gene="murD"
                     /locus_tag="ACG80L_000488"
     CDS             complement(13703..15064)
                     /gene="murD"
                     /locus_tag="ACG80L_000488"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS5022772.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15078..16040)
                     /gene="mraY"
                     /locus_tag="ACG80L_000489"
     CDS             complement(15078..16040)
                     /gene="mraY"
                     /locus_tag="ACG80L_000489"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS5022773.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16066..18258)
                     /locus_tag="ACG80L_000490"
     CDS             complement(16066..18258)
                     /locus_tag="ACG80L_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5022774.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18259..18660)
                     /gene="ftsL"
                     /locus_tag="ACG80L_000491"
     CDS             complement(18259..18660)
                     /gene="ftsL"
                     /locus_tag="ACG80L_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS5022775.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18665..19624)
                     /gene="rsmH"
                     /locus_tag="ACG80L_000492"
     CDS             complement(18665..19624)
                     /gene="rsmH"
                     /locus_tag="ACG80L_000492"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS5022776.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19645..20076)
                     /gene="mraZ"
                     /locus_tag="ACG80L_000493"
     CDS             complement(19645..20076)
                     /gene="mraZ"
                     /locus_tag="ACG80L_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS5022777.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20246..20614)
                     /locus_tag="ACG80L_000494"
     CDS             complement(20246..20614)
                     /locus_tag="ACG80L_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS5022778.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20785..21741
                     /locus_tag="ACG80L_000495"
     CDS             20785..21741
                     /locus_tag="ACG80L_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5022779.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21814..21930
                     /locus_tag="ACG80L_000496"
     CDS             21814..21930
                     /locus_tag="ACG80L_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS5022780.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21985..23667)
                     /gene="recN"
                     /locus_tag="ACG80L_000497"
     CDS             complement(21985..23667)
                     /gene="recN"
                     /locus_tag="ACG80L_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS5022781.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23685..24134)
                     /locus_tag="ACG80L_000498"
     CDS             complement(23685..24134)
                     /locus_tag="ACG80L_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5022782.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24241..25059)
                     /locus_tag="ACG80L_000499"
     CDS             complement(24241..25059)
                     /locus_tag="ACG80L_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS5022783.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25068..25958)
                     /locus_tag="ACG80L_000500"
     CDS             complement(25068..25958)
                     /locus_tag="ACG80L_000500"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5022784.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25960..26184)
                     /locus_tag="ACG80L_000501"
     CDS             complement(25960..26184)
                     /locus_tag="ACG80L_000501"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS5022785.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26189..27526)
                     /gene="xseA"
                     /locus_tag="ACG80L_000502"
     CDS             complement(26189..27526)
                     /gene="xseA"
                     /locus_tag="ACG80L_000502"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS5022786.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27527..28381)
                     /locus_tag="ACG80L_000503"
     CDS             complement(27527..28381)
                     /locus_tag="ACG80L_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS5022787.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28478..28927)
                     /gene="nusB"
                     /locus_tag="ACG80L_000504"
     CDS             complement(28478..28927)
                     /gene="nusB"
                     /locus_tag="ACG80L_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS5022788.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28920..29345)
                     /locus_tag="ACG80L_000505"
     CDS             complement(28920..29345)
                     /locus_tag="ACG80L_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5022789.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29591..30655)
                     /locus_tag="ACG80L_000506"
     CDS             complement(29591..30655)
                     /locus_tag="ACG80L_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS5022790.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30827..31072)
                     /locus_tag="ACG80L_000507"
     CDS             complement(30827..31072)
                     /locus_tag="ACG80L_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5022791.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31211..31585)
                     /locus_tag="ACG80L_000508"
     CDS             complement(31211..31585)
                     /locus_tag="ACG80L_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS5022792.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31668..32582)
                     /locus_tag="ACG80L_000509"
     CDS             complement(31668..32582)
                     /locus_tag="ACG80L_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5022793.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32603..33406)
                     /locus_tag="ACG80L_000510"
     CDS             complement(32603..33406)
                     /locus_tag="ACG80L_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS5022794.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33439..34428)
                     /locus_tag="ACG80L_000511"
     CDS             complement(33439..34428)
                     /locus_tag="ACG80L_000511"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5022795.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34531..35022)
                     /locus_tag="ACG80L_000512"
     CDS             complement(34531..35022)
                     /locus_tag="ACG80L_000512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5022796.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35213..38020)
                     /locus_tag="ACG80L_000513"
     CDS             complement(35213..38020)
                     /locus_tag="ACG80L_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5022797.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38454..38747)
                     /gene="rpmA"
                     /locus_tag="ACG80L_000514"
     CDS             complement(38454..38747)
                     /gene="rpmA"
                     /locus_tag="ACG80L_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS5022798.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38760..39104)
                     /locus_tag="ACG80L_000515"
     CDS             complement(38760..39104)
                     /locus_tag="ACG80L_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS5022799.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39131..39439)
                     /gene="rplU"
                     /locus_tag="ACG80L_000516"
     CDS             complement(39131..39439)
                     /gene="rplU"
                     /locus_tag="ACG80L_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS5022800.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39459..39535)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39721..40362
                     /locus_tag="ACG80L_000517"
     CDS             39721..40362
                     /locus_tag="ACG80L_000517"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5022801.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40424..42055)
                     /locus_tag="ACG80L_000518"
     CDS             complement(40424..42055)
                     /locus_tag="ACG80L_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS5022802.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT        11860 a         9169 c         7153 g        13914 t
ORIGIN      
        1 aatgagtctt ctctatcatc tttcgttttt tgttgtaaaa acgtctaaaa atcctttgtt
       61 ttttatttag aaaaatagtc aattaacgtt ttttttgtta cacttactaa tgatggtttt
      121 ttaccatgaa aggatgatcg aatatgacac caaatcgaga agattatttg aaaatcattt
      181 tagaactagg tggagataca acgaaagtca acaataaaca aatcgtatct agtctagatg
      241 tctcagctgc atctgtcagt gaaatgattt ctaaacttgt gaaagaagag cttgtagaac
      301 attcccctta tcaaggtgta caattgactg caagcggact gcaaaaagct agcagtctga
      361 ttcgcaaaca tcgcttatgg gaagtttttt tagttgaaca tttagactat agctggaatg
      421 aagtccatga tgatgctgaa gtattggaac atgtgacttc ggaacatctt gcagatcatt
      481 tagaaaacta tttgaatcat cctgaacatt gtcctcatgg aggatcgatt cctaaaaaag
      541 aggaagttgt ccatgaagaa cgccgacaaa cattggagtc ctatcctgtc ggtacaaaag
      601 tgcgtatcgc acgagtgctt gatgaaaaag aattgttgga ttacttagtc actattgacc
      661 tgaatatcaa cgaagaatat gagatcgttg acatcgcagc ttatgaagga ccgatcacta
      721 tccaaaatga tagaaagaaa atccccgtca gctttaaggc ggccagcacg atttttgtcg
      781 ataaaatata aaggagtgta tccacatgtc taatgaaaaa aatgtattgt ttacgatatt
      841 cggcggtaca ggtgacctag cccagcgtaa attgtacccg tctttgttcc gcctttacag
      901 aaaaggaaat ttaggcgagc attttgctgt aatcggaacc gctcgccgtc cctggagcga
      961 tgagcactac cgtgaagttg tcaaagaaac aatccgtgca ttgaatcctt cagatgaaga
     1021 agcaactacc tttgcaagcc atttttacta tcaatcccat gatgtaaatg attcaacgca
     1081 ttatcagaca ttaaaagaat tgtctgaccg attagatgga cactatcaat taaacggtaa
     1141 ccgtgtctat tatttggcta tggcacctca attttttgga acgatcgttt ctcatttgaa
     1201 atcacaaaat attatgacaa aaaatggctt caatcgtttg atcatagaaa aaccgttcgg
     1261 ttctgattac cagtctgctt ttgaattgaa ccagcaaatc agagaagtat tcccagaaca
     1321 agatattttc aggattgatc attatttagg aaaagaaatg atccaaaaca tctcagctat
     1381 ccgttttgcc aataatattt ttgaggctca atggaacaat cgctatatcg acaatatcca
     1441 aattactttt ggcgaaaacc tcggtgtaga agatcgcggg ggctactacg atcatagtgg
     1501 cgcacttaaa gacatggtgc aaaatcatat tttacaggtt gtagcattac ttgcaatgga
     1561 accgccagta gcattttccg aaaaagaaat ccgtacagaa aagatcaaag cattgaaagc
     1621 tatccgcatt tataacgaag aagaagtgtt agaaaacttc gttcgcgggc aatatgctca
     1681 aggtgaattg gatggtgtac aattcaaggg ctatcgcgaa gaagacaaag tggatgctca
     1741 atcagagact gaaacattcg tcgcaggcaa attcactatc gataacttcc gttggtcagg
     1801 tgttcctttc tatgtacgta caggcaaacg tttgactgaa aaagggactc ggatcaacat
     1861 cgtattcaaa caagttcctg ttaatgtatt caaaacatct gtcgatgaac catgtgacga
     1921 tacgacactt ccacctaatg ttctaacgat ctatattcag ccaactgaag gattttcttt
     1981 atccttgaat gggaaagaag tagggcaagg atttgaaacg gaaccaatca aattggaatt
     2041 tagaaatagt gcagaaatgg ttgaaaatag cccagaagct tacgaaaaat tactattaga
     2101 tgcgttgaat ggtgatggaa cgaacttctc tcattgggaa gaagtcgctc aatcttggca
     2161 tatcgtggat gtcattcgaa aagcttggga ccaaacacaa ccggattttc ccaactacaa
     2221 agctggatca atgggaccta aagcagcttt cgaactactt gaaaaagacg gattcgaatg
     2281 gatctggcaa ccagataact ggtatcgtga acgtggaaaa ttaaacgatt gataacaaaa
     2341 gggggtgtga caaagaacat gtcacacccc cttttccctt ttacttttct tcgaatcctt
     2401 cagctacagc ttcaggatag aagtcttcaa cgatttttgc acaacgaccg catagatgag
     2461 gaagtttttc atctactcct acatctttac gaacttggcg acagcgatcg caagtttccc
     2521 cgtctgcttt ttccactgta atagcaacat catcaaatac catagcgtta tctggcactt
     2581 gttcatctgc tgccataact tcaaagtagt ctggtgaaac gatcaataat tgtgcaatat
     2641 ctgcatccaa agcttttaac atttcacgaa cttgttgttt tggataaaca gttagttttg
     2701 cttccaaaga tttaccaatc aatttgctgt ttcttgcttc ttccaatgct tttaagacat
     2761 tatcacggaa gtccatgaaa gcagtccagg tatccatcaa ttcttcttgg tttgcaaact
     2821 cttcataacc aggaaactcg cttaactgaa cgtactcctc ttcttctttc aggaatgacc
     2881 agatttcttc tgctgtatgc ggaatgatag gagtcaataa ttttgtcaaa gctacagctg
     2941 tttgatagaa aacagtttgc atgcaacgac gttgataatc atcttctgct tcaatataaa
     3001 ctacatcttt agcaaaatcc aggtagaatg acgatagatc aaccgtcaag aagttcataa
     3061 ctgtacggta aatatgcatg aagttatatt tgtcgtaccc ttcttctcgg atttctttga
     3121 ttacttggtt caatcgaacg gtcatatatt tgtctacaga acgtaaatca ttgtacgaaa
     3181 cagtatcttt tttaggatcg aaatcttctg tattcgctaa taagaaacgc atcgtatttc
     3241 tgattttacg atatacttct gccacttgac ttaagatatc catagaaaca cgaacatctg
     3301 cttcataatc tacactagat acccataagc gaaggatatc tgcaccaaat tgtttgatca
     3361 ctttttctgg tgcaatgatg tttcctaaag atttactcat cttacggcct tcaccatcta
     3421 aagcaaatcc ttgagaaata actgatttgt atggtgctac accattgata gccacactag
     3481 tcgtgatact tgagttgaac cagccacgat attggtctga tccttctaaa tacatatctg
     3541 ctgggaatga taattctggt cgctgacgta aaacagcttc atgagaagaa ccagaatcaa
     3601 accagacatc catgatatct ttttcttttg taaattcgcc attaggagaa cctgggtggg
     3661 taaatccttc aggaagaagt tctttcgctt ctttttcaaa ccagataatt gatccatgtt
     3721 cagcaaataa ttttgccaca tgttcgattg tttctggtgt gatgattgct tctccatttt
     3781 cagcatagaa aatcggaagt ggtactcccc atgcacgctg acgggaaatt acccaatcgc
     3841 cgcggtcacg gatcatatta tacaaacgtg ttttacccca tgggataacc cactctactt
     3901 tttctacttc atccaaaata ttctgacgga atttatcgat agatgcaaac cattgtggag
     3961 ttgcccgata gatcacaggc tttttcgtcc gccaatcgtg aggataacta tgagtaaaga
     4021 aatctagctt cagcaaagct cctttttctt ttaatagatc agtgatcata ggatttgctt
     4081 tgtcataaaa gataccttca aatccaggtg cttcttctgt aaacaatcct tttgagtcta
     4141 caggtgacac tacagatagt ttatatttgc taccagcgat atagtcatct tccccatgtc
     4201 ctggagcagt atggactaat ccagtaccag cgtctaaagt aacatgatcg cctaaaatca
     4261 caagtgattc acgttcatag aatggatgac ttgcagtcat atattccatt tcttgtccag
     4321 caaattcttt tagaactgtt acattttccc aaccgatttc tgattggact gtttctagca
     4381 aatctttcgc tacgacaaac ttacggccat cggcttccac caatacatac gtaaattttg
     4441 gatttacagc aataccttgg tttgctggca atgtccatgg tgttgtcgtc caaatgacaa
     4501 aagctgtatc ttcatcaaga atatctttgc cgtctttgac attgaaagct acaaaaatag
     4561 atggagattt tacatcttta tattcaattt cagcttccgc taaagaagac tcacttgacg
     4621 gagaccagta aattggtttc aatcctttgt aaatatagcc tttttcagcc attttaccaa
     4681 atacgcgtac ttgtgcagct tcataagaag gatctaatgt aacataagga tgttcccaat
     4741 ctcctgcaac tcctaggcgt ttgaaatctg ctcgttgctt atctacttgg gaaagtgcat
     4801 attcacgaca tttttccaag tattctgcca tggacatttc tttacgtttc acgcctttat
     4861 tagccaatac ttgttcaatc ggcaatccat gtgtatccca tcctggaaca tatggtgcac
     4921 gatagccaga cattgatttt gaacgaatga taatgtcttt actgatttta ttcaatgaat
     4981 gtcctaaatg gatattacca ttggcatatg gaggtccatc atgaagaaca aagctaggtt
     5041 ttccttcgtt caactcttgt cttttttggt agattcgatt ttcttcccat tctttttgcc
     5101 actcgccttc tcggtttggc aaattaccac gcatagggaa ttttgttttt cctaattgca
     5161 aggtttcttt cattttcatt caaccataca ctcctttatt tgttttccgg acacaaaaaa
     5221 gcatgttcag ccgcaaggga cgaacatgcc gtggtaccac ccaaatttag ttgcatctat
     5281 ctagtgcaaa ctctctgtga ccaataacga ggtcagccgc tgcttcttac tctaatttcg
     5341 gaaaagcagg tctcaagtag aggatctttc accatgcagt gtcttaccga acttccactc
     5401 tcatcggctc gctttaagaa taacaaagtg cttttctgtt ctactcgttt tcattttcac
     5461 caggcacgat cgagatatca atagcctgtg tatcaaatgc cgtcataggt gcttctatga
     5521 ctcctgtgtc tgagtttaca tcagtatcat tttcattgtc aagctgtttt gctaaaactt
     5581 ctttgatcac ttcatggctg tcagtcacgt agctggagaa tggttgtaaa atttcatccc
     5641 attccggact tttcacttgt tctaattgag actcaagcat caagctgata cgttgatgga
     5701 atactcgtgt ttttttctta agatcttcag tttctgtcgc aagctgtctt gctttttctg
     5761 ttgcatctgt caagattttt cttgcttttt cttctgcatc agttgttaat tgatgtgcac
     5821 gtttttcagc gttcgcaact aactcatctg ctttgttttg tgcagaagta acgatcactt
     5881 cggattcctt gcttgcactt gtttttactt tatcagccgt atcttgagct acgatgattg
     5941 attggttcaa agcatctttt aattcattga agtactcaag tttttcttca gaatgtttga
     6001 ctgctttttc taattctcga tttctttgag tcaattcctc atagtcacga acaactaaat
     6061 ctaagaaatc atctacttcg tcttgattat atccccgcat ttttgtcggg aatgtcttat
     6121 tttggatatc taatggagtt aaagccatga gtaaaccacc tttttatttt atttacgtaa
     6181 tacaccgaac agtaccctgt acttttcttt tttcgtcttc ccctcaagtt cttggatctg
     6241 gactctgcca taacctctga ctgaaataat atctaatagt tcaagtacaa aatcaggtct
     6301 tgtattttct gtccagttca cttttacttt accagcttcg atcagctgct tggaccgctg
     6361 tctggaaata ttataaatct cagagatcac actgtctagt cgcatggaac taactgttcc
     6421 tctttcttgt gtccagctat ctttcggcat aatcatttct gtgtatttga ttggttccaa
     6481 gcgtacagca acctttccaa tctttgttac ttgtgcagca acaaaatgat ccacctcatt
     6541 tgcaataaat acttgccagc gatcaccgtc cgagatgata tcgccaaagt aagagcgttt
     6601 gatccccgtt cccactaaag ttccaaggat tttcccatga cttaggctgg caaatttgct
     6661 aggataatgg atttcataca atgctgtttc aaaatcttcc tctacaggtt cgtaataatc
     6721 ggggaaaatc aaacatcttt ttctttctgc ctgttcatat ccgccataaa acatgaaacg
     6781 caaatcactg ctttgtcgaa tcagtgtttc taaaatatag gattgtcttg ggtcaagaaa
     6841 ttcggtcaga tagggtgcat attgtccttg aacctgttca atccaatcgc ctaccgaatc
     6901 gataaatgta cgttcatctg cccgaaagtg ctggtataca tttgcatcca aaattttccc
     6961 ctcctatcta ttctgagaat aaagattttg ttgcttcaat caataaacta aatggacagt
     7021 tatccatata acgatctgtg ctaaaccttg tacggctaag ttcaagatca ggattgcccc
     7081 taaaattgtg aaatccaagg gtccaatcct caaaggcaga cgatcgaaca aactgatata
     7141 cggttcgcag atcttagcta ataatcgtcc tagccctgat tgatacccgc ctggaaacca
     7201 agagagcaat gcatatacca tcaagataat gctatagaaa taggctgccc gttgaagtaa
     7261 atgaagtaat agtgcaatag tataaatcgt agttcctcct cttacagatc aaacaaattc
     7321 gcatctgcaa aagattgagc ggttgaactg tcaatctcca tcccagtcgg tgtacaaaga
     7381 aaaatctcat ctccaacacg tttgatatct ccatctagtg cataaactgt tcctgttaga
     7441 aaatcaacga ttcttctcgc ttgtttttct tctactaaat gaaaattcac taaggttgct
     7501 tctccagcaa tgattcgttt agcaatcgcc atagcttcag aataagctct tggttccatt
     7561 acagtgattt ttccagcaag atttgcttta ggttgtgtag aggttcttga agctgttctt
     7621 tctttcgcta cagtttttgt aggtgattga tgtagttcaa ttactttttt cttttcagcc
     7681 ggctgaatac tttcatgcgt tacaggacgt gaagtcgccg ttttttgttt ttcatgagta
     7741 ttcgcagcag atacagaatg agttgattga ttctgtgttg tatttttttt agttggctgt
     7801 acagcctttt ttgttgtttc aggataatct tggtattcgt actcttcctc atcagctaat
     7861 ccgaaaaaat tagcaattgc tcctctgttt aaaagcggca tttttgttcc tcctttattc
     7921 gtctttgaat agcgctgttc ctacacggat aaatgttgca ccttcttcaa tcgcaatttg
     7981 aaagtcttga ctcatcccca tacttaattc actacatggt gcatgggcat aaccttttgc
     8041 ttgaacaaga tcacgcaatt ctttcagcgt accaaacact ttatggattt gctcatcaga
     8101 agcttcttta ggcgccatag tcatcaaccc aacaaccttg atttttgtgc aggcagctaa
     8161 agattccaca aacggtaaaa gctcatctgg tgaaattcca tgttttgatt cctcaccaga
     8221 aacatttact tcaataaaac aggataattc tttctctaac cgcttctgga tttcttctgc
     8281 taatcttaaa ctgtccaacg catgaaagta atcaatttca tttacaatca attttacctt
     8341 tctgcgctgt aaattaccaa ttaaatgcca ctttatttca ggaaaatcaa ataaatcttg
     8401 tttcttttct aataatttat ctactcggtt ttctgcacaa ttcataacgc cagctctaac
     8461 caattcttca gcttgagcgg attcaacaga ttttgttaca gctactaatg taacttcttt
     8521 cggatcacga tgaacaagag cacacgaatc ctgaatttct tgcttgattt tctgcaagtt
     8581 gtcagcaatc atgtgctctt cctctccttt attttttacg acggaaaaac ggtggcgtat
     8641 ttaattcatc atcgccatga gattttgttt cttcgcggtt gaatgtatca aattcttttt
     8701 tttcgatatt gtcaaaattg gaatcatcaa ctctaggacg aacattttgt tcacgtctga
     8761 tatcccaatc gccaaaactg ttttcttctt ctgaagaagt tggtttagct tgatccatat
     8821 ctaatacagt cttttgagca ggtgattgca tttgtgcttg tctagctggt cggctagact
     8881 tgcgttcctt ttttgattca tcgatacctg ttgcgatgac cgtcacacgg atttcatcgc
     8941 ccatttcttc attgatagat gtacctagaa tgatatttac atcgccagtt gctgcatttg
     9001 ctacgatatc tgaagcatct tgtgcttcga acaatgtcat atccaaaccg cctgtgatgt
     9061 taagaagtac ttgttcagct ccatcaatag atgtttcaag caatggagag gagattgctt
     9121 ttttcgttgc ttctactacg cgatcttctc cactagccac accgatcccc atcaatgctg
     9181 taccttggtt ttccatcact gttttcacgt cagcaaagtc aaggttaacg taacctggcg
     9241 ctgtgatcaa atcagagata ccttgtacac cttgtcgtaa aacattatct gcttcacgga
     9301 aagcttcaag catcggtgtc tttttatcta caacttctaa taaacggtta tttgagataa
     9361 ttaataatgt atccacattt tctttcaaac gagcgatacc ttctgcagca aaacggccac
     9421 gtttcggtcc ttcaaaagtg aatggacgtg tcacgacacc aacagtcaat gcgcccaatt
     9481 ctttcgcgat acctgcaacg attggtgcag cacctgtacc ggttcctcca cccattcctg
     9541 cagtgatgaa aatcatatct gcaccgtcta aagcttcgcg taaggattgt tcactttctt
     9601 ctgccgcttt ttgtccaact tctggctgag aaccagcacc taaaccacga gtatatttgg
     9661 ggcctaattg gataactgtt tcagcttttg aattttttaa tgcttgcacg tctgtgttgg
     9721 ctgtaatgaa ttcaacacct ttaacgtttt cttcaatcat tcggtttaca gcatttccac
     9781 cgccaccacc gactccgatg actttgatta ctgcgccgtc attgatatta ttatcaattg
     9841 agaattccat atcttattcc tcccaggatg aattaatcaa aaatatttgt aaagaaaccc
     9901 ttgattttat tttttacatt ttctccggac tcttttggtt ccggctcatc gtagattgtt
     9961 tcttcaggtg cttcatagct ctcatatgtg tttgttgttt cttgttcaac taccatttga
    10021 cgagctgctg aggtttcacc cgtaacagca atttttgcca attgatacac ttcactcaag
    10081 tttgctgaat aatccacgat acttattaca tttgtaaata ccgggtttct cagccccatg
    10141 tgatttggaa catacaattt tacatttacg ccgaagattt cttgtgccaa gtccacaaca
    10201 ccaggaagac tagcagctcc gcctgtcaat acgacgccac ctggcaactc taatgcttcg
    10261 atttggtcca atgcatcttt tgctttgttg aaaatctgtt ccattcgagc tgaaataatt
    10321 tctgacaagt agcgttcatc gactttcact ggttctgact gaccaatcac atctactgga
    10381 aattcttcat ctggtgatgt ccgttctggg tatgcatccc cgtagtttat tttcaacgct
    10441 tctgcattgt taaatgacgt atttaataca atagagatat ctttagtgac aaattcgcca
    10501 ccttcttggt caagattggt aaacttcaat tgcttatcat gcataacagc cgttgttgtt
    10561 tggccgccac ccatatcaat gacgatcgta ccaaaatctt tttctccgtc agaaagaatt
    10621 gattcagtca gcgctaaagg agtaatcacc atttcgttga cgattaatcc ggcattttcc
    10681 acgcatttac gtatgttatg gatgatcgtt ttaggtcctg taaacaacag accgtacatt
    10741 tctaatctta cgccgatcat tccacgagga tctttgattc cctcgaaccc atcgacagta
    10801 aaatcttgtg gcaatattga cacgatttga cgttcaggag gtattgaacg aacaagggca
    10861 gctgaagcca catttctgac atcttcatcg gtgatttcct ttgaatctcc gttaactgca
    10921 atcattccct ggcaattttc tacttcaagc atgtttgctg gcaaaccgac acttacgcct
    10981 ttgatctgga tacctgcttt ttcttctgct tgtcgtactg ctcgttgtat cgcttggacc
    11041 gttttatcga tatcaacgat aatgcctcgg ttaatccctt ctgattttgc gtttcctact
    11101 ccaataatat tcatttggct gtcgatatat tcagccacga caactttaac agatgtcgtt
    11161 ccaatatcaa ggcctacata cattcctgtt tttgccatga atgggattcc ctcctactta
    11221 ctattccata tacaagcgaa caactagcgt ccgttttaaa atatacttat taatagatat
    11281 attcactgct tccaattcta ccataaatta ctggttttta gaaagtacat tcttactttt
    11341 taaagttgtt ttatcaattt tcatttaaac ttgacggagt tgtacttgga ttatcttcta
    11401 tttgacttgt ttcacttgaa tcatttgaat cagatgatcc gtttgtgcta gacgatccag
    11461 gtgtttgtgc tttttgcgtt tctgcctctt cacttgattc gtttgtttga tccgcatttt
    11521 cttcactttc agttgtttct tccgattggc ccgattcttc cgtttcttga gtgctaccgt
    11581 aaggatgcga gaagatacca acttccatgt cgatcacacc tttttcatcc atatctttgg
    11641 ctacttgcga ataatatttc atttggttcg caagattatt gatatttaca ataacttgat
    11701 tgccgtcatt catattcaat tggatcagat ttttattgct tgctcttggc gtgtacttga
    11761 tttcagaaat tgctttttgt aattctgatg ataactgatt gtattacttt gttaattctt
    11821 tgatttttgc agagtctttg aactcttcca agatagggag attttttgta ggatttgcta
    11881 ctttttcatc taatactgtt ccattttcaa tcactgggta gtattgattg ttttttgcga
    11941 tcaaagcaat ctctttatat tctgttacat ccaaatcgaa ttcattcatc cctttgaaat
    12001 gtatatttgc tgtttcgatt cttggctgtg cttttttaag gcgatcgata taagtagatc
    12061 gatgaaaata ctgtccccaa acattttctc ctaatgacaa ttttgtatca gagatgattt
    12121 cttggctgtt gactgtctta ttgcctgata ctgcaactgc ttgtaatttg ctataaggag
    12181 aaacataata gatcaaaaaa atcagcggta tacccaatac agtgatgatc aaagctaacc
    12241 gacgaaatag tactttgtgc cgttgacttt ttaaattggg cagccggttc aaaaaagaac
    12301 cgttatatgg tccaccgttt ttcttttgct tgatctcttc aaaaaattcg atttttttct
    12361 cggactggct ttcttcttgc tgttcctctt gaaccgtttt ttcttcttca gaatctgaaa
    12421 ggggtgtatc taatgttatg tcttcttttt ttggctcttg gtttttcatc tcttgttctg
    12481 ccagatattt tcggttagcc ttttgccaag gagtcaaatt gttttcatca gaagacggat
    12541 gagacaattt ttttggttct tttttcttac tcactaagac caccgccttt attttttgat
    12601 tatttgtttt actaagtcat aaagacgttt tgaagcatca gggattcctt gttctttaga
    12661 agctcggcac atttgctttt gcaattcttc atcacccatg atttcgttga ctgtctgact
    12721 tagtgattca cctgttagtt cattatctgc aatcattttt gctgctcctg cgtgaacaag
    12781 actcattgca tttttagtct gatgatcatt cgtaacatat gggctgggaa ccaacactgc
    12841 cggtaacccc agagctgtga attctgcgat cgaagttgca ccagcacgcc ccaccagcag
    12901 atcactggaa gccatgacct ctgccatatt tttgatataa ggctggacac taatattttg
    12961 tatttcagga tttattttat ttaggatctc ttcataatat ctatctcctg aagcatacaa
    13021 aacttggtaa gaagaaggaa tatattcact taaaaaaact gatactgctt gattgatttt
    13081 aagtgctcct tgactaccgc caaaaatcaa gactgttttc ttttctggat ccaaatcaaa
    13141 tgttttcaaa atatctgatt ttttagtatc tgctacttct tgtgctcgag gattacctac
    13201 aagaactgct ttgttttcag gaaaaaagga agccgcatct ttaaatgaaa gagcgatccg
    13261 atccacatat cggcttaaaa atttattggt aacacctggt acactgtttt gttcatggat
    13321 aactgttgga atacccattt tggctgcagc gtacactacc gctccggaaa catatcctcc
    13381 agtaccgata acgatatctg gttgaaaatc actgaggatt ttttttgctt gacggatact
    13441 cttaagaaac aatcggaccg tcttgatgtt atgcatggaa atttttcgtt taaatccttg
    13501 aatctccaag gtatggaaag gcattcctgt atcagggacg attttgtttt ctagacctcg
    13561 ttttgctccc acatacatga attcagcatt cggttcttga gtctttacat aattgacaaa
    13621 ggcaagagct ggataaatat gtccccctgt tcctccgcct gtaaccaaaa ttttcatttt
    13681 ttatcgctcg cttctacttt ttctatttta atcggttcac agcttccatg aatctgtttc
    13741 ctctagtttc aaaatttggg tattgatccc agctggcatt cgctggtgat aataaaacta
    13801 catcaccatc tgtactgttt tctaatgcta aaggaactgc ttgttctaca gaatcggctg
    13861 ttactattgt ctcgatgcct gctttttttc cagcatccat caacttttct tttgtttggc
    13921 caaatacgac cattgctttg attcctttta atgaaggaat taattcatca aatgtatttc
    13981 ctctgtctaa tccgccagcc aataacacga ccttagacgt ttcaaatcca cttagagcca
    14041 ttttagtcgc taaaatattg gttgcttttg aatcattata aaattttctg ccttggattt
    14101 ctcctacata ttgcgtacga tgtggtacgc catggaaaaa atgcaacgtt tctcgtatag
    14161 cttcatttga tataccatac aattttgcta cagaaatagc agctagtgca ttttctacat
    14221 tatgcttacc tggtacgcca agttcagata tttccattat tttttctttt ttatagtata
    14281 ttgagtaatc gtcagcatac acaccgtctt ccaacacttc ttttgtcgag aaaggcagta
    14341 cgcgtgcttt tgttctttgc gctaattctt gtagttctga ctgattccag ttaaggatca
    14401 gataatcttt ttctgtcatg tttttttgaa ggttccattt agctttgaca tattcttttc
    14461 ttgtaccatg ataatcaata tgtgcttcat agatattggt aatcaccgct atatgagggc
    14521 ggaaatctgt gattcccatg agttggaagc tagacaattc catcacaatt ttatcttctg
    14581 attttgcttc ttgcgctaca ccacttgctg gatacccaat attacccgct aaacgtgcaa
    14641 ttccttgatc tgctcctgca ttcagtagta gtcctgtcat cgttgttgtc gttgtttttc
    14701 cgttcgttcc tgtaatccca ataattgggc attccgctac ttcgtaagct agctcaactt
    14761 ccgtaattac cggaataccc atttcttggg cttttgctac taaaggatga ctgtagggaa
    14821 tccctggatt tttgaccatc aatgaaaatt cttcatccaa caattcgatt ggatgactac
    14881 ctgttattac ttttatccct aaagaaagca gctcttgtgc ttccggattt tcgtcaaaag
    14941 gttttccatc attaacggtg acaagtgcgc ccaattcatg taataatttg gccgcactta
    15001 ctccgctttt tgcgagtcct aagacaagca ctttcttatt ttcatatgtt gatatttttt
    15061 tcatttcccg tcatctccta ccaaacgatc cataatgtga tcaacgaagt cacaatgctg
    15121 atcaaccaaa aaactgtatc aatcttccac tccgaccatc cgcacatttc aaagtgatga
    15181 tgaattggag acattttaaa aattcgtttt cctgtaagct taaaggaagt aacttgaagc
    15241 atcacacttg ctgtttccat aacatagatc aaaccaatca aaagcaatgt ccattcttgg
    15301 tttagcatta tagaaattgc cgctaaaagt ccgcccaacg ctaatgaacc gacatctccc
    15361 ataaaaatct tggcaggttt tctgttgtag acgaagaaac ccagtaaacc tcctaaaaca
    15421 cttaagcaaa tgattaatac atcatactga tgctgatgcc aagcaattag accgtaagct
    15481 gcaaaagaga tacttcctaa gccagcaact aatccatcga tgccatctgt cagatttact
    15541 gcatttgaaa atccaacaag ccagaagaga gcgaaaatac cataaatgat tcctaaaggt
    15601 agatctagtc cgaagaaatt caatacacct ggataacctt cagaacggta aacaagataa
    15661 aagatgattc cccctagaat ctgtccaatc aatttctgct tcgagttcag tcccatgttt
    15721 cttttcttga atatttttat aaaatcatca agaaagccga ttactccgta taacgctaat
    15781 acaaaaagca agatgaaaag agtaggagtt agctgttttt gccaagctcc aacccaaatt
    15841 cctgtcaaaa tagagccgat cagaaacact aagccgccca ttgtaggagt tccggctttg
    15901 ctattgtgcc actttggtcc ctcctcacga atcgcctgac cttgcttttt catttgaaaa
    15961 taaccaataa atagcggcat tgcggcaatt gtcattgcac aactgctgac aatgggaatc
    16021 aatgcttgtg tccattccat gctcttttct ctcctatttc tcttcttatt cttctaaagt
    16081 aaaactaagt ttgtcttcag tgattttttc atatggagca agttcttgtt tgacacaata
    16141 accatctcca tcaaaactaa cttcaatacc gaggattttc cccagcttca tgatatctgc
    16201 ttttgaccat cctgttacgt caggcatcag cttatcacca ccagtatata ggatcagttt
    16261 ttctccagag atcaactgat caccgttggc agtagattgt ttttgtactt ttttaccatt
    16321 accgatcact acaggttgca gtccactttt ttgagcatca gctgctgcaa cgtccgcttc
    16381 aagattacga taatcggcta cggatacttt ttctgttttt gtttctgtat ctgaatcttc
    16441 ttcagtttct ttaaaatcca ttgcccgttt catcaatgga tttgcgattt tagccaatgc
    16501 cattcgatca tatgtcttag ggtgtttcat tgttagatac aagacatagt ctggatcttc
    16561 agaaggaacc atctccacaa tcgaatacag gtaggctgta tctcctgttt gatatccccc
    16621 tgtatcagaa gcgatttggg ctgttccgtt ttttgccgat acattgtatc caggtacact
    16681 atatacacca taagcactac cgtagttttc gctttcgaca acatctctca tatattcacg
    16741 gactttttct gttgtttctt ttgaaaaagg ttgccctaga acttctgttt gagttgttcg
    16801 ctcttctcca gtttggggat ccaccacttt gctgatatat cgaggttgaa tcattgtacc
    16861 gttattcgca atagaagtga aagctttcat catttggaaa ttcgtcacac ctaccgcttg
    16921 tccataagca ctcattgctc ggtcgacaat atttaatgta ggtaaagcac cgtttacttc
    16981 atcatctaat ccagaatgag tactttgtcc aaatcccagt ttttgtaaat agttatacca
    17041 tcgtccgcct aatcgctgtt ccagtatgac catcccgacg ttacttgacc atgaaagagc
    17101 ttgacgcatg gtcagcacac ctttttcacc aaagtcatgg tcattgatcg tagcatcttc
    17161 tacttggatt tttccagatt ggaaggtttc attctcgttg aattctcctt cttcgatagc
    17221 agctgcagta gtgaaaactt tcatggttga tcctggttca taactatctt gtaccaagaa
    17281 attacgccag atagcatctt ttccagtcaa tccttccatt gtttcaggat tgaaagtagg
    17341 tcgttgtccc attgccaaga tttctccggt ttttgctttc atcaggactg ctgtcaattc
    17401 ttctggctga tattcttcat ttacttgatc catcagtgtt tctaaataac tttgtagtct
    17461 gctgtctaaa gtcgtgtaga tatcttgtcc gtcgacagct ttttcttctt ctgccactgt
    17521 acctggcaga ggattttgaa aattatcttt ttgataaata atcttaccat ctttaccgct
    17581 caagatatca ttgtaagcag actccaaacc tagtttacca actaagccat tctcatcttt
    17641 gtctggaact gcgtatccaa taaaatgaga agaaaagacg ccatttggat acatccgagc
    17701 ctgatggtcg acaaaataaa gaccagcgac accttctttt ttcatgtccg cttcgatgtt
    17761 ttgcttggtt tcttgggaga tatttttccc atagctacca aattcaactt gatacagctc
    17821 tttttttgcg ccgtcctcta aaatattcag tgcatctttt ttgtctatag aaagattctt
    17881 atgcaggatt tctgctattt tatcaaaatt cttttcttca acatacagtt ttttatcccc
    17941 agaggtatat gtttttgaga gtatcgcttt aattgaataa gaggaggcat cttctgcgag
    18001 cgccacaccg ttacgatcat aaatcgttcc tctttttgct tttactactt cacttccttg
    18061 gtacaactgt tttgtttttt cagctaatga ggtaccagca acatgacctc caataacgat
    18121 atacgagaaa cggacggcaa ataaaaagaa caatccaata ctcgtagcaa ataaaatgat
    18181 acctactttt ttgcgattgt tcatcggatt tagatttttc ttttccataa accgccgaat
    18241 tttattttta agactcattt acttcacttt ccttaaattg tcgtcattaa tagacaatcc
    18301 tttttcttct gcgatttgtt taatccgttc agtacgagaa agttcgtttt tttcttgttc
    18361 taaactcgtt ttttcttgct ttttatcatt gatttctgtt tgtattgacg tgatttcttt
    18421 ttcagctccg ctgatatttg tgcgcagcat aactgttaaa acacataaag caacaatagc
    18481 aatcgccacg cttgccacag caattttttc caaagcagaa atctgcttta atcgttttgc
    18541 cggtgattgt ggaagaatga tttcttgatt cgaagaagaa tcaggcattt gttgttccgg
    18601 ctgatccacc aattcaggta aatcatattc gtaatcttgc attttcttta attctgccat
    18661 cattctactc ctctttcatt cttatttttt ctgcaatacg taactttgca ctgcgtgatc
    18721 gattgttttc tgttagctct tcctctgaag gaaggattgg tttacgagta atgactttca
    18781 gttctggttg gaattcgtca ggtatgacag gaagtcccgg tggtagatct tgtaccgtac
    18841 tatattcttt aaacatactt ttgacaattc tgtcttccaa tgaatggaat gtgattacgc
    18901 tgattcttcc attgactttc aataaccgga ttgcctgttc taaagaggcc tcttcggcac
    18961 cgagttcgtc atttactgca atccgcacag cttgaaaaat tctttttgca ggatgcccgc
    19021 cttttcttct tgcgggagct gggatgactt gtttaatgat ttcgacaagc tcaccagttg
    19081 tttggattgg ttttgttttt cgtactcgtt cgatttcacg agcaatttgt ttggaaaatt
    19141 tctcctcgcc atagcggaag aaaatcttta ctaattcatg gtaggaatat tcattaatta
    19201 cgtgataagc ggataaagga gctgactgat ccatgcgcat atccaacggt gcatcttgat
    19261 gataactaaa tccacgttct gcttcatcta gctgaggaga ggaaacccct agatcataca
    19321 aaatgccgtc taccttatct acatgctcac tcaagcgttc ttctaattct ctgaagttcg
    19381 ctttgataaa tgtgaccata ccttgttcga cataagaagc taaacgcagc ttggcgtgat
    19441 cgattgcttt ttgatcttgg tcaaacgcat acagatgccc tttagaactt agttggctta
    19501 ataaatattc actatgtccg gcaccgccga gtgtacagtc tacataaacg ccatccggtt
    19561 tgatgttcaa tccatctacc gtttctttca acatgactgt atagtgttga aatgtttcag
    19621 tcattagtca gcctcttcct attcttatag tccgaaatca atcatacttt cagcaatttc
    19681 gtcaaagttc tcttctgctg ctgcagaaaa atcttgccat cgtgtctcat cccaaatttc
    19741 aattcggtta gatacaccga tgactacgca accttttgtc aaagctgcat aatttcttaa
    19801 cgtgctgggt atattgattc gtccttgttt atcaagttcg cattcagtgg cagccgaata
    19861 gaagaatcgc acaaaagtac gtgcgtcttt tttagccaac ggcatttcgt tcagtttagc
    19921 ttccagattt tcccattcgc tcatcggata gccgaaaaga cagccatcca atccgcgtgt
    19981 cagaacaaac ttttcgccaa gttcttcacg aagttttgag ggcacgatta aacgtccttt
    20041 tgcatcaata ttatgttgga attcacccat caacattgaa agccccccat tcctccacta
    20101 actaaaatta gtctaccaca atcccccact ttctaccacc ttttctggga aatccgggaa
    20161 caactttttt tattcataaa cattttttta aaaatcaagc tgcaaaaagc cttttaaaag
    20221 actttttaca gcttttctat ttttttcata ggtaagaaag tatgttcaca atgatcaaga
    20281 cagcgtacaa gacgattgtc aacagaaaaa ccaaacgcca agtcatcttg aaataacgcg
    20341 gataaataat ttcttcataa taatatgctt ggaaaacagc aatacaaatt cccattagca
    20401 aaacagaaat aaagaaaaac ggtaagatag aaattgtccc tgtatttcta gacaatgcat
    20461 gcataccgcc ccacaaaaaa ggaatcgcca aatcaggggc ttttatttta aaacgacgat
    20521 ttaacgaaaa taattttaca agaaattggc aaccaaacaa aacgattacc gggtaaatat
    20581 accaaaacaa gataagtggt gtgaatgtgc ccatgaacgt aaactccttt tttatcgttg
    20641 cttcaagtat actgtatata ctttacacaa ctgtttcatt ttatttaaat ttatgcagtt
    20701 atattctata tcttacaaac acattccatg tattccaatt ccctatccgt tataataaaa
    20761 gaaacaggca acaggaggaa aatcatgatc aaatatttta cattagataa tgagaaactg
    20821 cgtaataccg gaaaggaaac tgaccaaacg atctggtatt gtgtagaacg tcctaatcaa
    20881 gaagagattc atcagctgac acatcaattt gaaattccgt cagattacat cacttccata
    20941 ttggacgatg cagaaaattc ccgtacagaa gaattcaatc aatcaaagtt gacaaagcca
    21001 gcgttattat tgttacaata tccttttgct aaaacaagtc ctagcggtta ttttcaagtg
    21061 gatacatacc cgctctctat tattttgacg cccaaaaaga agcttatcac aattacgaat
    21121 catgaaccac tgtttttaaa gaaagtcttt agccagacct ttccaaaaag tgatatttcg
    21181 cctaatctca atatcttctt tcaattcatt tggcaggtca cactctctta caacagttat
    21241 ttagctacgc ttcaatctca atgcgatact ttggaagcca aacttcaagt atccactgaa
    21301 aacaaacaac tttatcaaat catggatatc caaaaaagcc tcgtttattt cagagaagcg
    21361 acttctgcta atttagatgc attgaaagaa ctagcgcaaa ataaactttt ccaagaaaat
    21421 catgctttga aaaatcattt acgtgatgta ttggtggaaa cagagcaggc aatgactact
    21481 tctcgcattc aattaaaact agtagaacaa atgaatcaga ctttttcagc aatcgtatcg
    21541 aataatctga ataatattat gaaaatttta acttctttaa cgattatttt aactataccg
    21601 acaatcgtcg gcagtatcta tggaatgaac attaagttac ctattgctga aagggatgat
    21661 gcctttatat ggctagttct attaacatta tttatttgta tcgtaactac ctattattta
    21721 aaaaaaggga agtttttata aaaaagccct cttgtggaaa tggaaaaaag attgtacact
    21781 tgaatcaact aattaatgac gaggttgaaa acaatgcgtg aaaaaaaaca aacaagtact
    21841 tttgctaaag taacaaaatt tgtcatttgg acaatgctga ttttaacgat tggatctgtt
    21901 gtattgactg ctgtaatgag tgtcatgtaa aaataaaata tagctatcag ctgctgaaaa
    21961 ggacctgaac cactgcgagg tggctcaggt cctttttgcg agtttcaata gttcttttgc
    22021 atgttcgagt gtgagttttg taatctcact tccagccagc atacgtgcaa tttcttcttc
    22081 tctttctttt gttttcaact ctgaaacaga agtttgcgta cgtccatcaa tcactgcttt
    22141 cacaatataa tattgatagt cggcgactgc agcaacttga ggcagatgag tgatgcacaa
    22201 aacttgagag tatttcgata tttgagaaat tttatctgcg atggcctgtg caactcttcc
    22261 gctcacacct gtatccactt catcaaaaat aatacttgtc acaccttgct cagaagaaaa
    22321 aatagttttc aaagcaagca acatccgaga cagttcgcca ccagaagcga ctttcaccaa
    22381 aggcttcagt ggctctcctg gattggtcgt aatgtaaaat tcgatttggt caaatccatc
    22441 atgattcaat tgtctatttt cttcttttaa gaaacgtacc tcaaactcag tgttttccat
    22501 atacagactt ttcaattcat gcagaatgga tttttccaat tcttttgcga tttttttacg
    22561 cgcttcatga agatctgcgg cttgttgctg aagtagaagc tctttttgct ctagttcctc
    22621 tttcattttg tccaattgac cttctgaaaa atcagaagaa gctagctctt cggtgatttc
    22681 gtcatagtaa gccaatattg actcaatgga ttcgccgtat ttacgtttca actgtcggat
    22741 taattctaaa cgttgcgtaa cttcttctaa acgactctca tctaactcca ataaatcaat
    22801 ctgacgactc atatcgccga ttgcatcttg cagcaaatag taagcacttt ggatattttc
    22861 ataaattgct tcatactcca catctagatg ggcgatccct tgcagttctg aaacggccaa
    22921 gccaacacca tctacactgt tttgttcatt attcgtcagt gctccgtaac ctgcagctaa
    22981 tccatcagca attttttgat aattgctaag tttttctctt tcttctttta atttctcttc
    23041 ttctccgact tgcaaatcag cagcagctat ttcttcttgc tggaaatgga gcatatctat
    23101 tctctgaaca taggattttt cattttgctg cacttttcgt accctttttt ccagctcacg
    23161 ataagagata tattcttgct gataactctt tttcttctgt tggaaagcta catctccaaa
    23221 acgatccaat aaagcaagat gtgattcagg ctggagcagt tcctgatgtt cgttttgtcc
    23281 ttggatatct actaaataac tgccgattct tctcagatta gctaatgtga taatatgacc
    23341 attcactcga caaacatttt ttccagttaa ggacatatcc cgtcgaacaa tcagattgtc
    23401 ctcatctgtt tcgatcccta attcaaccat cagttcagag aatccttctt gttttggtag
    23461 ttcaaacaac ccttcaagca cacatttttc tgctccttga cgtatataat cgcttgaacc
    23521 tcttcctcct gctaacagac ctaaagcatc gataataatg gatttacccg caccagtctc
    23581 tccggtcagc gcggtcattc cttcgtgaaa agaaagccgc aattccggaa taatcgcaaa
    23641 gttagtaata ctgatttctt gtagcatcac attcgctcct caacttaaag gtaatgcagg
    23701 aagtctttct tcaataaaac tgcctggtct tcagttcgtg taatcatcaa aacattatcg
    23761 tcatcattga taatcgaaaa taattctttc ttgtaacgtt tatcaatcaa atttgctgct
    23821 gccgaagcat ttcccggcaa tgttttcaaa ataacaaatt tttccatttg gtcaactgcc
    23881 acgaaagcgt cttttaatag tttttccaat ttagtattta catcttcgtt tgtttcagct
    23941 ggtatgctat aacggtagcc gccatctgaa gccggtacct tgatcagttt cagttccttt
    24001 atatctctgg aaatcgtagc ttgagtaaca gatattcctt tgcttttcag gatttcaaca
    24061 aattcttcct gctttcggat atcttgctca ctcaacaatc gagtaatcaa acgatgccga
    24121 tctttttttc tcattacgtc ttcgctcctt agcatattct tcttaatcaa tgatagctta
    24181 tttccttggt ataataaaga agaatccgca tacttctcac ttgattttca cagttacaat
    24241 ttatttatgg tcaaactgtt cgtgtgcatt tgaaaccact tctgccatat taatagctga
    24301 attgattgtt ccgctttcgg gtactttcct caagtgagcc aagaactcga tatttccttc
    24361 tccccccgtg atcggagaat aacttaattc cagcacatca tatccacctt gtgccgcaaa
    24421 tgacaaaatg tcttctaaaa ctttttgatg ggtctgaggg tcacgaacaa ttccgttttt
    24481 ccctacagct tctctgcctg cttcgaattg gggtttgatt aaagcaatca cttctccctc
    24541 ttcttttaaa atagcatgta atggaggcag catcagtttt agtgaaataa aagaaacatc
    24601 gataacagca acttctggaa ttcctaaggt aaagtcttcg ggttttgcat aacgaaaatt
    24661 cactctttcc attacgacga ctctaggatc ttgtcttatt ttccaagcta gctgattgta
    24721 gccaacgtct aaagcataac tcatttttgc gccattttgc aaagctgcat cagtaaatcc
    24781 gccagtggat gcaccaatat ctaacaatgt ttttccttca acagatagat caaatgcttt
    24841 gagtgctttt tctaatttta atccccctcg agaaacataa ggaagttttt tacctttgac
    24901 tcttaattca ctactcactg gaattttttc tcccggttta tcaaaccgct cgtttttttc
    24961 gttgtaaatc aaaccagcca tcactgaacg tttggcttgt tctcttgttt caaataatcc
    25021 ttgtttgacc gctaatacat ctacgcgttc tttttccatt ctttccctca tttctccaca
    25081 tcaaattgct tgatcatctg gcgcatcagc ccgccatcaa aagaaaaaac gtcctcttct
    25141 aatttgtcta tgatcttgtt ggcggataac agctcaatct ccaacgcctc acgtgtcttt
    25201 tctaggccta ataaacgcgg gtaagtattt ttttccatac gttcatcttt accagctgtt
    25261 ttccctaatg ttttcgttgt accaatcaca tcgagtagat catctcttat ctgaaaagct
    25321 aaacctaaat gttcggctaa tcgttgaagt agcaataaga tttcttcagg ttgcttcgct
    25381 aatatgccac cagccagcaa ggcataacga atcaaatgcc ctgtctttct ttcatgaata
    25441 aatgctaatt cttctaaaga aagctttttc gattcgccct cgatatctgc agcttgccca
    25501 gcaaccatac cttgtgatcc cgcacaaaca gctaattgct gaagcagcag aagcttagga
    25561 gaattaccta agtgtgccat actgattagc tggaatgctc ctgtaagcaa accatctccg
    25621 gccaatatag ctaatgcttc gccgaatact ttatgattgg taggttttcc tctacgaaga
    25681 tcatcattgt ccatcgcagg caaatcatcg tggatcaagg aatacgtatg caccatttct
    25741 aaagcagctg ctacttgata gacgggaaca tcaataggct catcaaagga agcaacggcc
    25801 gttaaaagta ataagggacg tattcttttc ccgcctgctt tcactgaata actcatcgca
    25861 tcatagagtc ctctatccgt tgtatgctca tctaaaaaag acaagatttc tttttctaca
    25921 gcaggtagat gaatagctga aaagtcagtt aatctcatct cagctttcct cgtttccgtc
    25981 aaaatcaact tcttcatttg attctgtcat cattttcgtc aaggtctctt ctgctttagc
    26041 taatgtatct tggcaatgtt tgcttaattc cattcctcgt ttaaacgaat ctagagctga
    26101 ttctaatgga acgtctcctt gttctaactg catgacaatt ttttctaatt cttgcagtga
    26161 ctcttcaaaa gttggatttt ccatattttt attctccttt gtctatctct tgtacttgtc
    26221 ctttaacgat tccatcttga taatggatcg tcagcaaatc cccttgatga atctcggtga
    26281 cactttttac tacttgatca tttaacgtcg tatagctgta gcctcttccc atgattttca
    26341 atggactcaa caaatcaaga gattgaacag cttgagcaaa acgctgctgc tgttgtttca
    26401 tatattgttg gattttctct tctaagcgtt tttctagata agcagtttct tggcgagctg
    26461 ctttaactcg ataaagcgga gccacttgtt ccaattggtg gcttaatgcg atcgtttgtt
    26521 tttctttcgc atgataaatt tgctgtgtag cttgatataa tcgctgtttc aactggtcta
    26581 atttgatggt ctgaccttca tacaatcttt caggctgccg aaatacatag gaacgtcgag
    26641 cacgctcgaa ccgttcttgt ttgcgctgga tctggcgtac aaacgcctgc tgcaatctag
    26701 cttgccgttc ttgaatgcgc atcaactctt ctgacaaaac aggaacagct aattctgcag
    26761 ctgctgttgg agtggcagcc cgaacatctg ctactaaatc agcgatcgtg gtatctgttt
    26821 catgtcctac agatgaaatc accggcgtat tagcttgata aatcgcccga gcaacccgct
    26881 cttcattaaa cggccataaa tcctcaatcg aaccaccccc acgaccgatg atcattgtat
    26941 cgaaattgcc catctcttct actctctgga tattcctgac aacattgtcc gctgcttggt
    27001 ttccttgtac aactgttggg aacaaaacca attggacaat tggatatctt cttttcaccg
    27061 tcgtgatgat atcgcggata accgctccgc ttggactggt aaggaccgcg attcgctttg
    27121 ggaaacgtgg cagcattttt ttaggccctt gaaaaaggcc ttccttcatc agcttttctt
    27181 ttcgttcttc caattcttga tataaagcac ctattccatc aggctccatg tgctctacat
    27241 agatttgata agaacctccc gcttcatata atgaaatacg cccaacgaca aggactttca
    27301 ttccttcttt tggctggaat cgtaattttt ggaatgctcc tttgaacata atagcagata
    27361 tttttgcatg gtcatctttt aagctgaaat attgatgggc attggcacgt aaacggaaat
    27421 tcgatatttc gcctgttaaa tatacgcgtt ctaaataagg atcggcatca aattttcttt
    27481 tcaaatactt cgttaatgta gtgacagtta aatattcttg tgccatctat tcacctgctc
    27541 caaaagcttc ggcagatttt acggtttgat acatcagcat cgtgatagtc attggcccta
    27601 caccctttgg cacaggagtg atccagcctg caataggctc cacttcatca aatttcacat
    27661 caccgatcag cttaccgtta gcatcacgat tcatccctac atctatcact acagcacctg
    27721 gttttacgaa ttcttttgtg acaaaatgtc cacgtcctat tgctacgact aaaatatctg
    27781 cttcttgtgc cagttcagcc agattttcgg ttttagaatg agcaacagta acagttgcat
    27841 ctgccatcaa gagtagctga gccatcggtt taccgacgat attgcttcgt ccgataacta
    27901 ctgctcgttt acccgccgga tcgatagaat aagcttcaaa cattttcatg atgccgtatg
    27961 gagtacaagg aatcttgtcc ggtttacccg caaggagtct gcctaaattc atcggatgaa
    28021 aaccgtctac gtctttttca gggcggatag ctaacaggat cttttcttca tctatatgct
    28081 ttggcaatgg taattggacc aatataccat gaaaactagg atcttgattg tatttttcaa
    28141 tttctgcaag tagttcgtct tcagaaatcg tctcaggata acgctctacc aatgaatgga
    28201 ttccgatttc ttttgctaca cgttctttat ttcttacata gatttggctg gctggatttt
    28261 cccctaccaa taaaacgact aatccgggat ggataccatt atctttcaat ttttggattt
    28321 tttcagcaat ctctgcctgc attttttcag ctaattcttt tccgttaatc aattctgcca
    28381 tctaaccact cccagatatt ttttgtgttt tcattctacc atagaatttt cccgatttat
    28441 aacaggttta tgatgattca tagaaaaaag ctaggactta cgccccagct tctaattctt
    28501 tcaatacatt ggataaaaca ccgttgacga atttacgtga acggtcatca ctaaatgttt
    28561 ttgccaactc gattgcttca tttaaagcaa cagccgctgg tacatctgtc acataagtca
    28621 tttcaaaaat cgcgatacgc aagatcacca aatccatttt tgagatccga ttgatgtgcc
    28681 aattgttttt taaatgtttt tcaatcactt tatccaattc atcttttttt gcacagacac
    28741 cgccaactag cgtatcaaga taaacaggaa caaactctga ttcatcttcg ttgatcatgt
    28801 cgcggtggtc taattcgatt gcgttgaaaa tcgcatcttc ttttgttagg tctgcattga
    28861 agtctaatgg aaataaagcc tgcaatgcca tttcccgaat ctcgtgtctt gataattctt
    28921 tattcattct cttcctcttc gtcttcaaaa agttcttcga ttgttggttg ttctaatttt
    28981 tcaggaacaa cagctactac atggatattg acttcagata aatcgatatc cgtcatgaaa
    29041 aggacttgct ggctgacccg ttcctgcatt tccattgcta ctttaggaac tgaaacacca
    29101 tattctagat aacagtaaag atctactttc aagccatctt cctcgtttgc taagtaaaca
    29161 ccttttccgt gagctgcacg tcctaataat tcagtgacat tgctggcaaa agttccgcgc
    29221 attccgtata cgccttctac tttagaagct gcaatcccga taattacttc gatgacttca
    29281 ggagcgatca cgatctctcc taaatcttgg tttgcgttta aaatcaaatt tttttcatct
    29341 gccatttatt tattcctccc tttgcctaag cacgagatac atccgaattc tctatgatgc
    29401 ggcatagtga tcacgtggta agaaaactgc cgccgatcag ctgcaaactg ctatgaattg
    29461 gagaaaacac ccaactttat tatctcttac agcttaaaag ttcttctata gtgtatcatt
    29521 aaatcgagtg attttctata acaaagtctg tgaacactga tttttacgct tacttcttct
    29581 attgatgaaa ctataagatg atcaattctt ttggtgaacg agtcaacaca cgatttccat
    29641 cttctgtgat caacaagtca tcctcaatac gtactccacc gattcctggt aagtagatcc
    29701 ctggttcatc agtgatcaca tttcctggta caaattgctt ttctgcacga gaagaaacgt
    29761 ttggtccttc gtggatctct aaaccgatcc catgtcctgt agaatgtcca aatgcttccc
    29821 cgtaaccatg ttttgaaata taatctctag caactgcatc taattggaca ccagtcattc
    29881 ctggttttgc agcatcaata actgctaatt gagcatctaa gactatttga taaatttctt
    29941 ttaatttttc cccaggatca ccaatagcga atgtacgtgt catatcggaa acgtaccctt
    30001 cgtaatagca accaaagtcc aatgtgatca agtcaccttg ttcgatgatt tttttgcttg
    30061 ctacaccatg cggcatagcc gaacgcaatc cgctagcaac gatcgtatca aaagaaacac
    30121 ctgaagcgcc aagagaacgc atataaaagt ctagttgatt tgcgacttca atctcagtca
    30181 tgcccggttg gatcattttc aggatgtgat catatgccat atccgcaata tgacatgctt
    30241 tttcgataat agcgatttct tcttcatctt tcacttctct gagttcttcg atcatctgtg
    30301 aaacaggtac taattccgca tcgatgattt cttctaaaac cgaatattct aaaaatgaga
    30361 ctgttgtttc ctcaaaagct aatgagtgca gttcttcttt gcgtactaag tttgctacct
    30421 catcaaaaat aggtccgaca tttttaataa tttcaaaccc ttgagcctgc gctgccgcct
    30481 gctcggtata acgaaaatca gtaataaaaa atgctttttc caaagtaata acagctaatc
    30541 ctgtcgtacc tgtaaagttc gttaagtaac gaagattgta cggactggta accaaaaatg
    30601 aatccaaatt ttcttcttgc atttttttgc gcaatttttc tacacgtagc atcataatga
    30661 aactccctct ttctttgcac gcttttttcc attatagcaa acttttcttc gtacacgaca
    30721 gttaaataaa aatttccgga aaacttcttt ttattttaaa taaataaagc caaaaaggag
    30781 tcacagtctc gctgcaactc cttttagttt actattttgt aaaaacttat gatcgttttc
    30841 caaagaaaaa tgagacaact gctaccaaaa tcactgcgcc taaaatagat ggaataagtg
    30901 ccatacctgc agcttttggt ccccattcgc cgaaaatcgc ttgtccaata cttgaaccga
    30961 ctagtccagc aatgatattc gttacacagc ccatcgaatt tcctttactg gtgatcgcac
    31021 ctgcaattgc tccgatcacg gctccaacaa tcaatgtcca taaccaactc ataatataac
    31081 ctccttcttt gctaatcaat tttagcatat gggatttaaa ggggacaaat aattggtttt
    31141 gctatttcat attttcttct gcaaagtaaa aaaataaaga aaacctcctc ttagaagttt
    31201 tctttatttt ttacttgcgt ttaaacatac tacgaatacc acgtttcact acatcaaaga
    31261 aactcaatga ttgtaccatt ctatcttcat ctacatattc tctgatcgca cgaagcactt
    31321 ttttaggttc tttggaagaa aatgtgtatg taccattttt tttcgtttga atggcatatc
    31381 gaggaatcca ttttcccttt agcatgactg atgctagtac ataatccact tcttcccatg
    31441 gaatttggat atatttgcgt ctatctcgcg catggtaaaa ctcaaaacca ttatcaccta
    31501 tcatgatttg cccgtaatct gtcagacccg taaacgaagt agcatcaatt accaagtcta
    31561 cttttgtatt gattgattga accattttct ccctccagat atttgtttat gaacagtata
    31621 ctatataaaa aaagctcggg acaacgcccg agctatattt ttatggatta taaaagtccg
    31681 atgacgtgtc caacaacacc gactacgaat aaaccaagga tgataacgat tggagaaacc
    31741 tttttcttaa gcaaccacat acataacaat gttaatccta atgcagctaa accaggaatc
    31801 aattggtcta agttgtcttg taatgttgtt accttttcag gagaaagggc taagccatta
    31861 ttaacttgtt caaatgcact cttgattcct tcaccacctg aaggcaattt atcccattcg
    31921 atgtaagcac ctttatctaa ttttacagtt gatacaactg gtaagaattt gattgatacc
    31981 catctttgta caagggcagc taatacgaac atccctaaaa ttgaagcacc ttttgttacg
    32041 tcttgtaaca atccgccaga aaggtcatca gtaattttag aaccagcttt gtagccaaat
    32101 tcttgtgtat accacatgaa tgcccaacga ataatgttcc aacctaagaa gaagatgatt
    32161 ggtccaagga tatttcctcc catagctaat gaagcaccta gtgcacctag cattggacgt
    32221 actgtaaacc agaacactgg gtcaccaaca ccagctaaag gacccatcat acctacttta
    32281 accccttgaa tcgctacatc atcaacaggc gcgccattcg cacgttcttc ttccagagcc
    32341 aatgtaaccc ctaaaattgg agaagcaatg tatgggtgtg tattaaagaa ttccaagtga
    32401 cgttttaatg ctgcagaacg ttcttctttt gttttgtata attttttgat tgctggaatc
    32461 atagaaaatg cccagccacc gttttgcata cgttcgtagt tccatgaacc ttgaatgaag
    32521 gtagagcgcc acgcaacagc taaacgatct ttttttgata atttgatttt ttcttctgcc
    32581 attttatttt cctccttcaa gattaatagt cattcaaaat gtcgcctaaa gggtcaccag
    32641 agttgcttcc gcctccatta tttgatgaac cacccatttt agaaaggttc aagtaaataa
    32701 gagctaatgc aacacctaaa gcacctagag caatcaatgt taattgagaa attgcagcta
    32761 caacgaaacc aataacgaag aatggccata cttcttttgt agccatcatg ttgattacca
    32821 aagcgtaacc tacagctacg accattcccc caccgatagc cataccatct gttaaccatg
    32881 ctggcattga ttcaaggaat gattgcacag tttgtgctgg gataaatagc aacgctgctg
    32941 ctgggaccgc gatacgaacc ccttgtaaac atacagcaat gatgtgccac atttcaacct
    33001 tacggatgtt tccttcttca gcagccgcat ccatcaaatg aacgattgga acagcgattg
    33061 tacgtacgat cattgttaag aacaaccctg caacagccaa aggaacggca atcgcgatcg
    33121 cagaaggaac acctttaaca ccttgtccac ctaaaactaa aataattgct gatgctactg
    33181 atgctaatgc agcatctggt gctacagcgg caccgatatt tgcccaacct aaagcgatca
    33241 tctgaagcgt accgccaagt acgataccag cttcgaggtt accggtaact aaaccgatca
    33301 atgtacatgc cactagtggt tgatgaaatt gaaattcatc aagaatacct tccataccag
    33361 ctagaaaggc aactaatacg actaaaatta ttgaaataat agacatgata agcctcctat
    33421 aaattttaat attatttatt acgcttttgc taattcagct tttgcttttt tcaaaatatc
    33481 atccatgtta tcgcgtgaat cattaggtac tttacgtaca tcgaatttca cacctttttg
    33541 ttcaagcttc tcaaacgctt caacgtcttc ttgtcccata gacaatactt tacttacaac
    33601 gactttaccg accgaatgcg ccattgaccc aacatttaat tctttgatgt ctacgccacc
    33661 ttcaacagct gtcaatacgt cttcaggatt ttcaaataaa agcaatgctt tggtatttcc
    33721 aaaacgaggg tcttttgcta cttcgatcat tttgctaata ggaataacat ttgcttttac
    33781 acctggagga gcagcttgtt cgattagtct cttacgcaga tcatcctttg atactgcatc
    33841 tgaaacaacg ataatacggt ttggttgcac tgcttttgtc caagctgtcg caacttgtcc
    33901 atgtaacaga cgtgagtcaa cacgtgctaa tacatatttg atttttccgt ctccgaccac
    33961 agttccttct ggaagtgctc ctttaggttg tgcgtcttca actgctgctt tcggtgcttc
    34021 tgccggttct aattcttccg gttttacttt tacaccgtct tttgctgttt caatgatgtg
    34081 tgctgcaatt tcatgagcag attccattga aaagcgtgat gcgtaggctt caatcaacat
    34141 tggtaaattc aaaccgctga cgattgccca tttatctttg tgctcttcga ataatgtgtt
    34201 tgattgattg aacggtgttc cgccccataa atcaactaag aataacactt cgtcttgatt
    34261 atcaaaagaa gcaaccgctt cttccaattt ggctctcaag tcatctggac cttcacttgg
    34321 tttcaaaatc acggctttga cgttttcttg ttcgccaaag atcattgaac cggattgtaa
    34381 gattccttca gcaaattgtc catgacttgc taggataatc cctaccatat ttatacctcc
    34441 tatttacttt tgttgtaaca aattcaaaaa tattaataat tgctaaccat tacaacagaa
    34501 ttatttgacc ttttccaaca accattcttg ctacaccttt ttgattttcg ctagtagttc
    34561 catcaaaagg attttcctgt cagcaggtac ttttcgaatc tctaactcca cttcttgact
    34621 gttcaagtaa cgaaaggaat caatatcttg ttgatttact gaaacaaaat tggtaatcat
    34681 tgatttgcct tcagtaaaac tcataccgcc gatattcaac gacttcaact gtacaccgca
    34741 gcggacaacc gcttcaacat cttggggatt ggtgaataaa agcatcactt ttgctcgtga
    34801 taataatcca ctatgataga tttgaatcat tttctgcacg gttattacat ttgctgtgat
    34861 ccccggcggt gcagcttctt ttagtagaaa ctttctcaat ttatcttgag cgactacatc
    34921 gctgatcaca atgatacgtt caacacctgt ttgcttcgac caaactgttg cgacttgccc
    34981 gtggattaaa cgatcatcta tacgtgccaa tcgaatatcc atctgcaaaa ctccttcttc
    35041 ctcactactt cacagaaacc cctatcattt ctgtgtgaac aaccacctcc tagcacacac
    35101 tactataagc aaaaagcgtg ccaacttttt tacgatacac taattgaatt taaaggattc
    35161 ttaaccaata cacaaaaacg atacactata aaagtgtatc gtttttgtgt attcatggca
    35221 cagtgtattc ttttttatct aactgattat ggactagacg tagcagataa tacatttctt
    35281 ctatcggcac ttttactttt atctgatctt ccaacagaac aactgaggct gccaattggc
    35341 tataaagagg atcattcgtt gtatttgtca attcgttttc ttccacagtc aatggttcat
    35401 tccgaataat acgttcgatc attcctgctg tgtgcatcac taggttgatt ttgaatccat
    35461 attcttcatc gccgattccc gtcgtctgag agatttgtgt agaaaattgc cacattggat
    35521 cgattaactt actaccatta ataaagatga agttatcaga gataaactca acacacgtat
    35581 ttttagctga ctcttcatct agggagatga attcagtttc ctccaattct gattccatca
    35641 gcaactgatc caaaatgact tcgatatttt gatcaataaa acgttctaaa ggaataaatg
    35701 gggcttggat attcaaatca gtaatcccag tagtagctat gatctgatat tctttttgga
    35761 taactggcag ttcttctttc acttccacaa tcgacaaagc tagaacttct aattctgttt
    35821 cttgacgctt gtttaccgct cgttcgatca attctttgat tctttgtgct gtaccttcgc
    35881 cagatgcgca gatagccaaa acagcttttt tcttatgaat aggttttgct tcctgaacag
    35941 attctacaga agatatttct gcgtaacctc gaaaattttt caatgattca tgcaacatat
    36001 ctagatctgt ccctaatact gagacctttc gtacagtttc caaaactaga gaagttgtta
    36061 ccatatcaac agtacgtact ggaattccag tgtctctttg aatttgcgaa ttaaacgacg
    36121 caagggaccc catatctact aagagtatga ccccgcttcc ttcatctact tcttgtacat
    36181 tgcgttcaat tcggtccaat gctgttttcg gatccatatc caaaggcatg tctactgctc
    36241 gtacgttctc tgcatctaac aactgttgga caacttggac catactgctt gctgtgctgt
    36301 ttccgtgagc tgcaacgact acaccgattc tgccgcttgc ttgatttgcc cgtaacgaga
    36361 cgagtagaac ggtcaagtaa tagtcttcac tttctggaat ctccacttga aaatacgttt
    36421 cgatataact acgcacttct ttcgcaacag catattcatc tggaaaatca aaagccatct
    36481 cacggatgtt atcattcgta tgccgttctt ctcctaagtt gatttttttc aaaaaagaac
    36541 tgatatgcaa gctcatagca taaataaagt tttgctgaaa ctcatatggt aaagatcttt
    36601 tgaccattac ataaatctga ttggtcactt ctatgatttt cggatctaca aattctgcta
    36661 atttgctgtc tgcattaaaa gaaaaaccat ggtctttata gaaagacttc agatggatgt
    36721 tgatatctgt agaaatgaaa tgattgattg cttcttgatc caaaccatcc gacttcagta
    36781 aagctgcttt atctccgatg atatcatata gattataagg taactcatag gagtccgatt
    36841 ggattttcat gatcgtatca tttggtgcta cagttatctt tggctccaat attttagaaa
    36901 gctctgccat tgcactgcgg ttgcttgcta attgaatcaa tccgctacga atcccttctg
    36961 tcaaatcatc gattgtgatc gagatttcgg gtgattgcat atgatttaga aatcctcgtg
    37021 cacatactaa ctggatattt gacttcaatt gtcctatatt tccataggtc acactgccga
    37081 tcaatgcttt tgctacatct tctgtcaaag aaatctttcg ctgcgtgcgg tttgcttcgt
    37141 gggcaatcat gatttttact aaatcgactt tctcatttgc aggacgtttt tcaaaagccg
    37201 gaagtttgat attgattggg atacgccgaa caaaagtttc taatagagaa gaacctgggt
    37261 cttctgtggt cgcacctacg atccgtacat ttgcttcatg ggatttcgtt gtttccccaa
    37321 gacgactgta agttccatga tccataaagt aaaagatcat ttcttgacct tcaggcggca
    37381 accggtgaat ctcatccaaa aacagcatac cgccatccgc ttgatcgata atccccattt
    37441 tttcttcatc tgcgcctgta aaagcacctt ttgcgtaccc aaataaatga ctcatcaaaa
    37501 gctcagggtt attggcataa tcagcacaat tgaaaacaat cagttccgtt tcctcatcta
    37561 ttacatgatg cgcttttgca aaatgataca tcgcgtgtgc aaaataagtc tttcctgatc
    37621 cggtaggtcc tgtaatcaaa caatttaatc ctcttggcgg atacaaaatc gcagcttttg
    37681 cctgttccac tgaatttttc atgctgccgt tagctccaat gatttttgca aagatatcct
    37741 tagtatctat ttggagtgta tttttttctt cagagaccgg cgtttctttg taactgacta
    37801 catgttttgt aactggcttt acgtgcgtaa cgagtgtttg atatatgtaa cgtactggtc
    37861 gtccgtctgt ctttatcagt tttccttcgc gcactagctg attcagatct ttactgctat
    37921 ttgttcgctg aatccctaac acttcagcaa tttcctgtgt tgttactcct tgatcatatt
    37981 ctgcctttgt taaatgagcg gttttttctt tgacatacat ataaattcgg tcaatgcgtt
    38041 ttgccaatgg tatcgctcct tttctataga ctattgtact taggcttcaa aacataatac
    38101 tatgtattta tttctgtttc gtcaactatt tacctgattt tgttttgttg ataacacgcg
    38161 gatagataga aatgttcaat ttaaaagaac gaaaaatccc gatacgattt ttataatgga
    38221 aacaagaaaa agaacactgt ttaccatata attatttcac tgatagcaaa caatatagct
    38281 tccatcaatt gaaaacgata gttatcaaaa catcctgatt ccaaattggc aaaaaaatat
    38341 taagtgtaaa aagacaaagt aaacttcgta gcctccacta caacttttat ttttataaac
    38401 aaaaaaggct gtgacaaaag tcttgtcaca acctctttaa tcataagatt caattaagct
    38461 tcttgagcta ctggataaac agacacttgt tttttgttac ggcctttacg ttcgaaacgt
    38521 actacgccat caacttttgc aaataaagtg tcgtctccac cgatacccac gtttacacct
    38581 ggatagattt ttgtaccgcg ttgacggtaa agaattgatc cacctgtaac tgtttgtcca
    38641 tcagcacgtt tagcacctag acgttttgat tctgagtcac ggccgttaga tgtagaaccg
    38701 ccaccttttt tgtgggcaaa taattgtaga ttcatagata acaacatggt ctgcacctcc
    38761 tatttatcat taattgtttt aatttggata tattctgagt tttcagcttc tatcgattgt
    38821 aatcctaata aaagattttc taatagaatc tcagcaatat tgttttgttc ttgtgtcatt
    38881 cctgaagtga cttcggtata aagataaccg ccttcttctt cattcatttc aacaatcggc
    38941 tcaaaaccgg ctaatgaggc aatcccgttg acagtgctga tcgctaacgc agaaacaccc
    39001 gcacaaacga tatcacttcc ataaggacca gcatcagcat gcccggtcaa agtgaatgaa
    39061 acgatctgac ctgcgtcatt tcgcttaaat gttcctttga tcatatatag gccttctttc
    39121 tcaaacgcta ttaagcgttg atcgcgtcga taaccacttt tgtatatggt tgacgatgac
    39181 cttgtttacg gtgtgaatgt tttttaggtt tgtatttgaa agtaacgact ttcttttgtt
    39241 tgccgtgttt ttcaacagta ccttcaacag ttgcacctgc aacagttgga gcgcctactt
    39301 ttgtagattc tccgcctact aagataactt cgtcaaaaac aactttttct cctgcttcaa
    39361 cgtcaagttt ttcaacatag attgcttgtc ccacttcaac ttttacttgt ttaccaccag
    39421 ttttaataat tgcgtacatg ctataaagca cctccttctg atatacttag actcgccatc
    39481 cgaagtgaca gcatatgctg tgcttattac ttgttctgag cggttgtagc tgtggagtcc
    39541 acaattacaa cagtactagc ttaccagaac agcaaccgtt agtcaactaa atttaaaatc
    39601 aaaaagtaaa acaaaaattc aagcaatact tctgcttttc tatcctcaca gttctaccaa
    39661 ataaaatagt cgcattgtgt ttcttttatt cttataataa aagaaaaagg aggagcataa
    39721 atggaatacc caaaagaact gatacaagaa gcttctttaa gaatgaacgg ccgtcctgta
    39781 gaaggattta tcgctgggca aggacctctt catcctaaac tgatgctcgt gggagaagcc
    39841 ccagggaaaa cagagattga tacgcatgtt ccttttagcg gtcaagctgg aaaagaattg
    39901 atgcaggcac tttcttcaac tggcttaaca cgagaagagg tgtatatcac cagtgctgta
    39961 aggagccgcc cgtatcgcgt gactcatcgg atcaataaac ggacacaaca gtcagaaact
    40021 gtttatccaa atcggacacc tactcgttcc gaagtatttg cgcatgcacc aatattggat
    40081 tacgaactga tgaaggtcca gccaaaatta attgcgacac ttggaaacat tggtcttcaa
    40141 cgtttacttg gcaaagaata tacggttacc aaaaatcatg gtcaactcta tacagggcct
    40201 gtcttacaat taacagaaca aaaggatgcc tacgaatggt cagaaaaaaa ctatcgaatt
    40261 cttccacttt ttcatccagc agctattttc tacaatcgct cccttgtacc tgttatccat
    40321 caagactggc aagtactagc ttcactttta cagcaatatt aaaaagaaca gacaagacca
    40381 taaaatcttc agtttacagt cttgtctgtt ctttttcatt gttttaatgg ctaattgcag
    40441 tatatttcgc atgtaaagct gtcaacgcat gagcaactgt ttgatgttga tgcgtgaatg
    40501 tatgttccca caagtgagaa agttgttttt ctacttctgg ctgcaaggaa gtatagataa
    40561 tcgtttgtcc tctttgctct gctttttctt gaaattcatc aaaaactgca cgtgccgtca
    40621 aatcaatact tggtacgcct cgcatggaga aaatgacacc atcgtgatga tttagttctt
    40681 ccaatttatt tttgagtttt tctgaagaca taaaaaacag tggaccacta atatacacta
    40741 ctgtccagtt attcagcttt tccgtttctg gcagccccat tctttgccaa tcgatcgttt
    40801 ctaatgaaat cgtgatagca gcgcttttga caacaaaaaa tacacagccg ccgatcaccc
    40861 cgatcacgat agcaatactt aaatcaaaga taactgtaca aatcatcgta aggataaaca
    40921 atagaactgc tgaccaatat ttattagtaa atagctcctt gatcgtttcc cattcattca
    40981 tacgccaagc cgtcacaatc aaaacacctg caagtgctgg cattggaatc tgtgacatca
    41041 ctggcgcaaa aacaagcatg gagagcagta agacaactgc atgtatcata ccagctaatc
    41101 tagtttgtgc accagatttg atggccacgc ttgtacgggc gatggccgca gtagcaggaa
    41161 tcccgccaaa gaaaggaagc aacagatttc caattccttg cgccattaat tcttgattgc
    41221 tatctaaggg cttatttgcc attcggccag ccgaagcacc acatagcaag ctttcaatca
    41281 ttcctaataa agcaatgcta atagctggaa ctgccacttg ttgcaaggca gaaaagctaa
    41341 aatctcccat attcaaacga ttactactga tcaatgtttg tggaatctta cccacagttg
    41401 ctactggtaa atggaacaat agcataactg ctgttgccaa aatgatagcc agcagcgaac
    41461 taggcattcg tttcgcccat ttttttggat agataaacat ccctaaaatg actagccctc
    41521 ccatgatcaa agaggctgtg tttatcggaa atcctatcgt ttgataacta cttagttttt
    41581 caataacgtt tgtaccttcc gagtgaacac caaataaatt atcgatctga cctaatgcaa
    41641 taatgatagc aatacctgaa gtaaaccccg taattacggg tgctgggata aaagaagtca
    41701 gtgttcctaa tcggaaaagt cctgctgcaa gtaaaaatag acccgctaaa aaagtagcaa
    41761 gcaatacacc ttgcattccc tttgttgccg caatagacat caagatagct gccatcgcac
    41821 ctgtcggacc tgatatttga taaaaaccgc ctgataaacc gccaataacg ataccggcaa
    41881 tgatagccgt aatcaaacct gcggccgcat cagctccgct tgatacccca aaagccaagg
    41941 ctaaaggcaa tgctacagca gctactgtga tcccagccaa aagatctttc tgaaatcttg
    42001 aaagatcata acctgaaaat tcttttttta gtgaggaaac ataatttttg aacacctatc
    42061 taactccttt ttcatgaaat tttctaaaat accatt
//
LOCUS       DAZUMB010000012        41982 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000012 DAZUMB010000000
VERSION     DAZUMB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.012"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..1867
                     /locus_tag="ACG80L_000519"
     CDS             257..1867
                     /locus_tag="ACG80L_000519"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS5022803.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1844..2056)
                     /locus_tag="ACG80L_000520"
     CDS             complement(1844..2056)
                     /locus_tag="ACG80L_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022804.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            2159..3028
                     /locus_tag="ACG80L_000521"
     CDS             2159..3028
                     /locus_tag="ACG80L_000521"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS5022805.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3193..4464
                     /locus_tag="ACG80L_000522"
     CDS             3193..4464
                     /locus_tag="ACG80L_000522"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5022806.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4461..5756
                     /gene="rho"
                     /locus_tag="ACG80L_000523"
     CDS             4461..5756
                     /gene="rho"
                     /locus_tag="ACG80L_000523"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS5022807.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5855..6118
                     /locus_tag="ACG80L_000524"
     CDS             5855..6118
                     /locus_tag="ACG80L_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS5022808.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6563..7012
                     /locus_tag="ACG80L_000525"
     CDS             6563..7012
                     /locus_tag="ACG80L_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5022809.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7069..8487
                     /locus_tag="ACG80L_000526"
     CDS             7069..8487
                     /locus_tag="ACG80L_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5022810.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8487..9347
                     /locus_tag="ACG80L_000527"
     CDS             8487..9347
                     /locus_tag="ACG80L_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5022811.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9521..10294
                     /locus_tag="ACG80L_000528"
     CDS             9521..10294
                     /locus_tag="ACG80L_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5022812.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10441..10806
                     /locus_tag="ACG80L_000529"
     CDS             10441..10806
                     /locus_tag="ACG80L_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS5022813.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(10954..12162)
                     /locus_tag="ACG80L_000530"
     CDS             complement(10954..12162)
                     /locus_tag="ACG80L_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5022814.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12181..12459)
                     /locus_tag="ACG80L_000531"
     CDS             complement(12181..12459)
                     /locus_tag="ACG80L_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022815.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12652..13308
                     /locus_tag="ACG80L_000532"
     CDS             12652..13308
                     /locus_tag="ACG80L_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5022816.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(13750..14547)
                     /locus_tag="ACG80L_000533"
     CDS             complement(13750..14547)
                     /locus_tag="ACG80L_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS5022817.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14544..15728)
                     /locus_tag="ACG80L_000534"
     CDS             complement(14544..15728)
                     /locus_tag="ACG80L_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS5022818.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(15753..17000)
                     /locus_tag="ACG80L_000535"
     CDS             complement(15753..17000)
                     /locus_tag="ACG80L_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5022819.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17215..18960
                     /gene="dnaX"
                     /locus_tag="ACG80L_000536"
     CDS             17215..18960
                     /gene="dnaX"
                     /locus_tag="ACG80L_000536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS5022820.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            18980..19294
                     /locus_tag="ACG80L_000537"
     CDS             18980..19294
                     /locus_tag="ACG80L_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS5022821.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19319..19915
                     /gene="recR"
                     /locus_tag="ACG80L_000538"
     CDS             19319..19915
                     /gene="recR"
                     /locus_tag="ACG80L_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS5022822.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            19979..20623
                     /gene="tmk"
                     /locus_tag="ACG80L_000539"
     CDS             19979..20623
                     /gene="tmk"
                     /locus_tag="ACG80L_000539"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS5022823.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20638..20967
                     /locus_tag="ACG80L_000540"
     CDS             20638..20967
                     /locus_tag="ACG80L_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS5022824.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            20981..21919
                     /gene="holB"
                     /locus_tag="ACG80L_000541"
     CDS             20981..21919
                     /gene="holB"
                     /locus_tag="ACG80L_000541"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS5022825.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            21955..22779
                     /locus_tag="ACG80L_000542"
     CDS             21955..22779
                     /locus_tag="ACG80L_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS5022826.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            22772..23119
                     /locus_tag="ACG80L_000543"
     CDS             22772..23119
                     /locus_tag="ACG80L_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS5022827.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23188..24060
                     /gene="rsmI"
                     /locus_tag="ACG80L_000544"
     CDS             23188..24060
                     /gene="rsmI"
                     /locus_tag="ACG80L_000544"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS5022828.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24169..25290
                     /gene="dinB"
                     /locus_tag="ACG80L_000545"
     CDS             24169..25290
                     /gene="dinB"
                     /locus_tag="ACG80L_000545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS5022829.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25344..25946)
                     /gene="nrdG"
                     /locus_tag="ACG80L_000546"
     CDS             complement(25344..25946)
                     /gene="nrdG"
                     /locus_tag="ACG80L_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS5022830.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26261..28450)
                     /gene="nrdD"
                     /locus_tag="ACG80L_000547"
     CDS             complement(26261..28450)
                     /gene="nrdD"
                     /locus_tag="ACG80L_000547"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS5022831.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            28776..29543
                     /locus_tag="ACG80L_000548"
     CDS             28776..29543
                     /locus_tag="ACG80L_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022832.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29533..31575
                     /locus_tag="ACG80L_000549"
     CDS             29533..31575
                     /locus_tag="ACG80L_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022833.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            31856..32005
                     /locus_tag="ACG80L_000550"
     CDS             31856..32005
                     /locus_tag="ACG80L_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS5022834.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32016..33530
                     /gene="dltA"
                     /locus_tag="ACG80L_000551"
     CDS             32016..33530
                     /gene="dltA"
                     /locus_tag="ACG80L_000551"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS5022835.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33527..34732
                     /gene="dltB"
                     /locus_tag="ACG80L_000552"
     CDS             33527..34732
                     /gene="dltB"
                     /locus_tag="ACG80L_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS5022836.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            34777..35010
                     /gene="dltC"
                     /locus_tag="ACG80L_000553"
     CDS             34777..35010
                     /gene="dltC"
                     /locus_tag="ACG80L_000553"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS5022837.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35013..36281
                     /gene="dltD"
                     /locus_tag="ACG80L_000554"
     CDS             35013..36281
                     /gene="dltD"
                     /locus_tag="ACG80L_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS5022838.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36422..37504
                     /locus_tag="ACG80L_000555"
     CDS             36422..37504
                     /locus_tag="ACG80L_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS5022839.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37504..38307
                     /locus_tag="ACG80L_000556"
     CDS             37504..38307
                     /locus_tag="ACG80L_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5022840.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38377..39117
                     /locus_tag="ACG80L_000557"
     CDS             38377..39117
                     /locus_tag="ACG80L_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5022841.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39128..40132
                     /locus_tag="ACG80L_000558"
     CDS             39128..40132
                     /locus_tag="ACG80L_000558"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS5022842.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(40177..>41982)
                     /locus_tag="ACG80L_000559"
     CDS             complement(40177..>41982)
                     /locus_tag="ACG80L_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022843.1"
                     /translation="FYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSW
                     VFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLG
                     EKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTG
                     PLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFE
                     AVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIF
                     IGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVA
                     IIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIK
                     NDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFE
                     MLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSF
                     RETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMM
                     MKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        13499 a         7367 c         8723 g        12393 t
ORIGIN      
        1 attttttttg cactcataaa ggacttaaga aataatttct caaaggatat cgaaaaagaa
       61 aaaattgtaa gcggatagta ttcatttgag tacttgactt tcccttagtc agcttgtatt
      121 atacaccttg ggcacccttt tagagggtca gaagtttaca gaaatttcgg ctccctattc
      181 ttattgaaga atagggagct ttgtttattg tctgagactt ttcctgcaca taaattaaaa
      241 aggagttttt gaacttatga caaaatatat ttttgtgacc ggcggtgtcg tttcatcaat
      301 cggaaaaggg attgttgcag cttctttagg acgcttattg aaaaaccgcg gcttaaaagt
      361 aactatccaa aaatttgatc catacatcaa cgtggatccg gggacgatga gcccatatca
      421 gcacggagaa gtattcgtaa cagatgatgg tgctgaaaca gatttggatc tgggccacta
      481 cgaacgtttt attgacatca atttgaacaa atactccaac gtgacgacag gaaaaatcta
      541 ttctgaagtt ttgcgtaaag aacgtaaggg cgaatacttg ggagcaactg ttcaagttat
      601 tcctcatatt acaaatgaga tcaaagaaaa aatcatgcga gcagctaaaa tgactgattc
      661 agatgtgatc attacagaag tagggggaac agtaggggat atagaatctt tgccattcct
      721 tgaagcactt cgtcaaatga aagcagatgt cggtacagat aatgttatgt atatccatac
      781 aacattgatt ccttacttga aagctgctgg agaaatgaaa actaaaccaa cgcagcacag
      841 tgtaaaagag ctacgtggtt taggtatcca accgaatatt ttggtggtac gtacagagca
      901 acctgtttct caaagcgtga aaaacaaatt ggcacaattt tgtgatgtcg aaccagaagc
      961 agttatcgag tcgccagatg tagatacact ttactccatc ccattagttt tacaagcaca
     1021 aggaatggat caaattgttt gcgaccattt aaaactagat acaccagaag cagacatgac
     1081 agaatggaaa gaattagaag aacgtgtctt gaacttgaag aaaaaagtac ggattgcgct
     1141 tgtcggtaaa tatgtcgagt taccagatgc atatatctca gtagttgaag cattgaaaca
     1201 ttctggtttc gcctataatt cagacatcga gatcgattgg atcaaagcac aagaattaac
     1261 gagagaaaat gtagaagaac gtttgaaaga cgcagatgga atcttggttc ctggtggatt
     1321 tggtgatcgc ggagtagaag ggaaaatcga agcgattcgt tatgctcgcg aaaatgacgt
     1381 tccgttttta ggtatttgtc ttggtatgca gatggcttgt gtcgaatttg cacgtaacgt
     1441 cgttggatta gaagatgcgt catcagctga aacgaatcca gatacagcaa acaatattat
     1501 tgatttaatg gctgatcagg aaaacatcga gaatttgggt ggaactttac gccttggatt
     1561 atacccatgt aaaatcaaaa aaggaacgaa gacggctgaa gcatatgatg gagccgatgt
     1621 tgtgcaagaa cgtcaccgtc accgctatga gttcaataac aaatatcgtc aattgtttga
     1681 agaacaaggg ctagtctttt caggtgtctc accagataac cgtttagtag aaatcgtaga
     1741 gctgccagag aaaaaattct ttgtgggatg ccaattccac ccagaattga tttctcgtcc
     1801 aaatcgcccg caaaaattaa tcaaagcatt tgtcggagca tcattagcag atcacgaaac
     1861 aaaataattt ttgaacttga tagaaagaga aaagtagcaa gagaagggga taagcaattt
     1921 tcttgtcact tttctctttt tctgatttgt tattatttgt aggaaaaaag atagaaagca
     1981 aaagactttt ttcgattaaa gcgctattgg atattttatt ttttgcaaaa aacttcttga
     2041 aaattttcat tttcattgat tttagtagaa aactaccgaa atgtttggta aaatagggtc
     2101 gttggttaag aattgtctta acaaatttag ttttacaaaa cttaggagga attttattat
     2161 gccagtagta tcaggagctg aatttttaaa agctgctcgt aaaggcggtt acgctgtcgg
     2221 cggattcaac acaaacaact tggaatggac tcaagcaatc cttgaagcgg ctgaagcaaa
     2281 acaagcacca gtacttatcc aaacatcaat gggtgcagct aaatacatgg gtggttacaa
     2341 aatctgtaaa gatttaatca ctgatttagt tgaagcaatg aacatcactg ttccagttgc
     2401 tatccactta gaccatggtg attacgaagc tgctttggaa tgtatcgaat taggctatac
     2461 ttcagtaatg ttcgatggtt cacatctacc atttgaagaa aacttgaaat tagctaaaga
     2521 cgttgttgaa aaagcacatg caaaaggtat ctcagttgaa tgtgaagttg gttcaatcgg
     2581 cggagaagaa gacggaatca tcggtaacgg cgaattagct gatattgaag aatgcaaaca
     2641 aatggttgct acaggtatcg actacttagc ttgcggtatc ggtaacatcc atggtcaata
     2701 tccagaaaac tggaaaggat tagcttttga ccacttacaa gcaattgctg atgctgtagg
     2761 tccagacatg cctttagtat tacatggcgg atcaggtatt cctcaagatc aaatcgaaaa
     2821 agctatctca atgggtatct caaaagtaaa tgttaacact gaattccaat tatcatttgc
     2881 tgcagctact cgtaaatata tcgaagctgg taaagataaa gaaggtaaag gattcgaccc
     2941 tcgtaaatta ttagctccag gtaaagcagc aatcgttgaa gatgctaaat ctcatatcga
     3001 ctggttcggt tcagctaaca aagcgtaagc atccacataa cagatacgat atataggaat
     3061 ggagaacgac ttttgaagtc gctctccatt cttttttact taaaacggca gaccatgctc
     3121 gtttcttttt tttatctatg ataaaataca agcatatcca tgaaaaaaga ttaataaaca
     3181 aggtggaaac agatgaaaaa aatcgtaatc aaaggaaacc gacctttagc tggtgaagta
     3241 acgatcagcg gtgcaaaaaa tagtgcagtc gcattgattc cagctgcaat cttagctgat
     3301 tccccagtga tcttggaagg tgtgccggac atacaagatg ttcattcatt aattgaaatt
     3361 ttagagatca tgggcgcaac tgtccatttt tccaacaaca tactagaaat cgatcctcga
     3421 gaagtcgtat cgattccgat gccgcacgga aaaatcaata gtttgcgtgc ttcttattat
     3481 tttatgggaa cactgcttgg taaatttggc gaagcggtag taggcctgcc aggtggctgt
     3541 tatttaggcc ctagaccgat tgacctacac gtcaaaggat ttgaagctct aggtgctgaa
     3601 gtgacaaatg aacatggtgc catgtattta cggacagaaa ataaaggatt gcgtggcaat
     3661 cggattttca tggacgtggt ttcagtcgga gcaacgatca atgtgatgtt agctgctgta
     3721 aaagcaaaag gacagacagt aattgaaaac gctgcacgag aaccggaaat catagatgtg
     3781 gcaactttac tgaataatat gggggcaaaa gttcgaggag cgggtacaga tgtgatccgt
     3841 atcgaaggag tcgaaacatt acatggctgt cggcatttca tgattcctga ccgcatcgaa
     3901 gcaggaacct atctagcatt agcagctgca gtaggaaatg gtatcaaagt gaaaaatgtg
     3961 atctttgaac atttggaaag ctttatagca aaattgcagg aaattggtgt ccatatgaaa
     4021 atcagcgaag atgaaatcga agtttacccc tcgaaagaat taaaacccgc aaacatcatg
     4081 acctatcctt atccaggatt tgccacagat ctgcagcagc cacttactgc gttactattg
     4141 atgactacag gcacttcaga aatcatcgat acgatctatg cgaaacgtgt gaaccatgtg
     4201 ccggaactag cacgaatggg cgctgatatt accgtggaag gaaatatgat catcgtgaat
     4261 ggtccaaata aacttcacgg cacagaagtc gtggcttctg atcttcgggc aggtgcttgt
     4321 ttagtgattg ctggactatt ggcagaaggg acaacgacga tttacaatgt agattatatc
     4381 ttacgcggtt atgatcatat tatcgagaaa ctaacagcct taggggcatc aattgaaatg
     4441 atagaggaag tgggagctga atgagcgatt atttgacaat ggcagaacta gagaacaaaa
     4501 cgttaaaaga agtttatagt tatgccaaag aatttaaaat tccttattac agtaagatga
     4561 ataaaaagga attatctcta gctgtgattc gtgcacaagc agagaaacaa ggattttatt
     4621 atatggaagg aatattggat atcgttggtc aagatggtta cggtttttta cggccaatca
     4681 attatggacc aagtgcagaa gatatctata tttctgcctc acaaatcaga cgattcagtt
     4741 tgagaaatgg ggacaaagtg gcaggaaaag cccgtccacc taaagaatcg gaacgttatt
     4801 atggtttgat gcacgtggaa agcgttaatg gaaaagatcc ggaagaagca aaagaacgtc
     4861 cgcattttcc agccttaaca cctctttatc cgcaaagaca gcttcaactg gaaacaacac
     4921 cgcaaagact atctacccga atgatcgata tcttttctcc aatcggtttt ggtcagcgtg
     4981 gattgatcgt cgcaccgcca aaagcaggga aaacttctgt attaaaagaa atcgcaaatg
     5041 gtatcaccga aaactatccg gaagtagaat taatcgttct actgattgat gaacgaccgg
     5101 aagaagtaac ggatctagaa cgaagcgtga aaggcgacgt ggtatcttct acatttgatc
     5161 tgcagccgca aaaccatact cgagtttctg aattagtatt ggaacgcgca atgcgtttag
     5221 tcgaagataa acgagatgtc gttatcttga tggatagtat tactagactt gctcgggcct
     5281 ataacttagt cgttccacca agcggacgta cattgagtgg cgggattgac ccagccgcat
     5341 tgtataaacc taagaaattt ttcggggcag caagaaacat cgaagaaggt ggcagtttga
     5401 cgattttagc gaccgcttta gtcgatacag gaagccgtat ggatgacgtg atctatgaag
     5461 aattcaaagg aactggaaat ctagaattac aactttcacg tgaattatct gaacgtcgga
     5521 tcttcccagc catcgatatc aaaaaatcag gtacccgaaa agaagaatta ttgatgccaa
     5581 gtgaagaatt agaagaaatt tggaaacttc gaaaaaatat gactggagat tcattagaat
     5641 atacggaaca actgatccgc tttttgcgta aaacaaaaaa caatcagcaa tttttcaaag
     5701 aatttcaaga tgtctccttt ggtaaaaaaa atccgaccag aaatctgaaa agataattgc
     5761 aaccttgact gaaacatgtt aagatgttaa tgttgtaaat cgacaaatca actctggttc
     5821 agcaaatgga gcagggcaaa ggagcgaaaa aatcatgaaa gaaaacatcc atccagatta
     5881 ccacccagta gtattcatgg attcaacaac tggtttcaaa ttcttgtcag gttcaacaaa
     5941 aacttcacaa gaaacagttg aatgggaaga cggtaataca tacccagtca tccgtgtcga
     6001 agtaacttct gactcacatc cattctacac tggacgtcaa aaattcacac aagcagacgg
     6061 acgtgtggac cgtttcaaca aaaaatacgg tctcaaagac gcaaacgctg cggaataata
     6121 aaaacgctga taactcaacg tttagcagac tatctgatag ggatagtctg tttttttatg
     6181 ttttttgcca atttaaaact tcgtttgatg ctgaaaaagt cgtaggatca taatgcacaa
     6241 aacaatgaaa aactagtata tagttttaat tatccacttg ataaaaacac cttatattaa
     6301 cgaaatgaag aggggttatt tcgaagtaaa aaaccattta cctaaaatta gaagctaagc
     6361 tttcctttca aggaaggctt tttgttttgt tatagacttg cgattgtttg tgagctagat
     6421 cgttcatttt aatgacttat acacgcggat gatgggtctt tttcctctgt tcattcataa
     6481 tattgaacgg tacagctagc gatgctatac tgacctcagt tccaagaaaa cgttaacatt
     6541 ttttgtgggg gagaggtttt ttatgaggtt tgatcaattg atcgcagaag aactgattgt
     6601 tttcgatcca tcgatagatt caaaaggatc attatttgag cgaatagggg atgtgttgaa
     6661 aaaaacaaat aaggtaagaa atgttcaaaa agtcgttaag gcactattca aacgagagga
     6721 ggagatctcc acaggaatag aagatggatt tgggattccc catgcaaaaa gcaaatatgt
     6781 gacaaaacca gcactgtgtt tttttcatac agccaaatta acggactata gcgggataga
     6841 tgaaatgcca attgaatata tttttgccat cgtagttcct gaaaaatcag cagatatgca
     6901 tctagagatt ctcagcacac tctccagacg attgatgaat gcagatttta gaaagggaat
     6961 aaaagaagcc actacgccag cagatatcat cagacagatt agtcaagaat gaaaatagta
     7021 gagtctttgt ccgagtatcg atagtgaaga aaaaggagga aaataatcat gaaaatcgtt
     7081 ggtgtaactg cttgcccgac tggaattgct catacttata tgtcggcaga aaaattagaa
     7141 gtaacagcaa gaaatctaaa ccatcaagca aaattcgaaa cacaaggtgt aaaaacagaa
     7201 aatcagttat ctgaacaaga aatcaaagag gcagatgcaa tcattcttgc agtagataaa
     7261 gaaatcgaat tggaccgatt tgccgggaaa aaagtaaagc gtgtctctac aagccgagca
     7321 atcaaagaac cgcaagtagt aatagacgaa gcattgagag atattggcgt attcgttgtg
     7381 tctaatgaaa aagaaccagc tgcaaatgag aaaaagccga caatctataa tcatttcatg
     7441 aatggtgtga attacatgct tccatttgtg atcgcaggcg gaatcatcat cgcactgagt
     7501 tttgcctttg ggattacagc ggctgatccc aactcagcag actataatgt gttagcagct
     7561 gcattttcaa ggatcggagg agatacagcg tttgccatga tggttcctgc gttaggcgcg
     7621 gggattgcta catcgattgc tggaaaagca ggttttgccc caggaattgt tgccggatta
     7681 ctggcctcta caggtggttc aggttttctt ggcggaatga ttggtggcgt gctagcaggg
     7741 tatatctgtg attttctggc aaataaaatt agcgtaaaaa aagaattttc tgctatttat
     7801 cagctgattg tcgtcccctt tttatcgatc ttaattattg ggcttttgat ggtatttgtg
     7861 attgaacaac cgatcacatg ggtgttggat gctttgacaa attggctgaa tagcttggga
     7921 aatacatccg ggctattgtt cggattactt atcgggatca tgatggcagc tgatatgggt
     7981 gggccgatca ataaatcgac ttcgactttc tcgatcggtt tgatgtcagc tggagtaaat
     8041 gcaccgattg ctgcttgtat ggctgcagga atgacacctc ccctaggtat tgctttagcc
     8101 actttattat tcaaacataa atttaccaaa gaagaacgga cttcaggtca atcatgctgg
     8161 attttaggcg cttcgtatat tacagaaggt gctataccgt ttgcggtatc tgacccgatc
     8221 cgcgtgatac ctagtttgat gacaggttca gcagtcgctg cagggatttc catggcagta
     8281 ggagttactt caatggcacc tcatggaggg atttgggtga tgctaattcc aaatgttatg
     8341 aacaatttac ctatgtatgt cttggcaatt gcatcaggta cagtcactac agcctttatg
     8401 cttggactat tgaaaaaaac agtgaaagag aaaaaacaag acaagtggac gaaaaatgaa
     8461 aatctaacaa atgaggtgga agcgtaatgt tatatacaat gaaagagtta ttgaaagtgg
     8521 ctaaagagaa tcaatttgca gtaccagcct tcaatatttg cagctatgat atgatgaaag
     8581 caatcatcga agaagcagag agattagaag caccagtaat cttagaaatt catcctgatg
     8641 aaatcgccta tttaagtgat gagtttgttg cggcagtaag agcctatgct tatacaagta
     8701 aactgccgat cgttatccac ttggatcatg gcggtacgat agaagatgtc ttacgggcga
     8761 ttcgaaacgg ctattcttcc gtgatgatcg atgcgtcttt gaaatcattc gacgacaatg
     8821 tacagattac gaaaaaagtg gttgcgttag ctcatgcggt aaatgtctca gtggaggctg
     8881 agcttggaac gattgggaat aatggttctg ctgaaggagg tgctgaaaca atcatttata
     8941 cagatcctga tcaagcgaag atttttgtga aagaaacagg aatcgataca cttgctgtag
     9001 caattggaac agcgcatggc ttgtatccga aaaacagaac tccaaaatta aatatcgact
     9061 tactgaaaga attgaaccaa caaatcgatc ttccatttgt tttacatggc ggttcaggta
     9121 atccggacgc agaagtcagc gaagcagtaa aatacggagt tgccaaagtg aatttgagct
     9181 cagatcttaa aagcgtcttt ttcaatgctt tgagagaaat attgagtgaa aatccatcga
     9241 tgtacgagcc gaatgcaatt tatccagcag ctaataaaaa ggtacaagaa gtggtcaaac
     9301 ataaaatgga aattttgaac acagtaggca aggctagttt atactaaaaa taaaaatgtg
     9361 tggtcaagag ggtatgacaa tagtcttgtc ataccctctt aaccacacac gatgttcaga
     9421 agtagttttt tcgaaaaatt tcttcttatt tctcgaagcc aatcacttct gtcacaacct
     9481 cttttttcct gttactatat aaagtaaatg gggtgatttt atgttgaatg aagtggagaa
     9541 tagacagcag gagattgtga attatctaaa aaacaaccaa tttgcaagag tcaccgattt
     9601 gatcgagtta gtaaattata gcgaagccac gattaaacgt gaccttgttt ttctggaaaa
     9661 aagtggattg atccgtagag ttcgcggtgg cgccatgctt attgacaatc gaaaaattga
     9721 cgtgccttac ctgatgaaga tcaccaaatt agaagaagaa acagaaaaac gtaagattgc
     9781 agatacagct tcttctttga ttcgagatga tatggtctta ttcttggatt ccagtacaac
     9841 ctctttacac ttagtcagaa acctagcaag atttgatggt ttgcagatca ttacgaatgg
     9901 catcgtcaca gctgctatgt tgagtgaata tacaacggca aaagtatctg tattgggagg
     9961 ggcaattgtt ccgaaacgag caacaatcaa cggttcaaaa gcctataacg atgcattgtc
    10021 atattatgcg gatttagcga taatgagttg tcgcgggcta gatttcagcc aaggagtaac
    10081 agaaactcat gaaggagagg caatggtcaa aaaagctttt agaaaacaat cagaccggct
    10141 tgccgtttta gttacacagc aaaaaattgc acataaattc atgcatcaag ccattgcttt
    10201 tcatgaaatc gattatctgg tgacaagtaa agaactgaca aaagaacagt atgcgaaagt
    10261 aaaggaacac catatcgaat gtctgtacgg ataaattatt acgtgaatgt tctaagtggc
    10321 ttcctcattt ttagatagga ccttatcttt gctcgcttct ctcagccgat ggtataataa
    10381 tgaaaaggtt atcttgttag ataatcttac ttttttatcc aggagaaagg agtgcagaaa
    10441 atgaaacatg tgaaggcgtt ggtagtgaag gcaatcatga tctgggcgat tctgtgggtc
    10501 gtgctgacag gtttatacgg tgttagcttt atggattcaa cgattgttgg ggtaattatt
    10561 gtagtcatga tctatgtgct aggggatctg ttgatcttac gtaaagtagg gaacatcgca
    10621 gcgacgatcg cagatgctgg ttcagctgca gttatcttgt ggctctatct atattttatg
    10681 aatgatacag tcgatatttg gatgaaagta ttgattcctg ctctattgat cggtgtggca
    10741 gaatggttct tccacaagtg gttgctaagt caaggcatcg taccagatga acggaaaatg
    10801 gaataaatca acagttaaac tattcttatc gataaatgaa agctttaaaa ataaatagat
    10861 gtttaattaa gaataatcat aaactatttt tactcaaaaa accgctcaga ttattttcag
    10921 atctgaacgg ttttttttat ctattcatca agatcaaatt tcatttttca atgattcaat
    10981 caattcgccc tctgcttcat gactatgttt tcggccatta acaagataga gtgctgtaca
    11041 tatcaatgga atgactccgg caatcacata caatcctcta aatgaagtaa gagattttag
    11101 catccccatc aaataaggac caacgcctaa gcctaggtct aaaccaataa agtaggtaga
    11161 taaagcaata ctgattcgat gttcgcggac gatttttaaa caaacagcct gtccatttga
    11221 cataaacgta ccataaccta ggccgactaa tgctccagag ataagcaatg tcacactatt
    11281 cgtcgttaca ctcagtaaaa ataatcctgc tgttaagaat aaatagctag gatacatcac
    11341 gtattgttct cctttgacat cgaaaatcct tccagtcaat ggacgtgtaa atgtgatgac
    11401 caaagcatat acgacgaaga aaaatgaccc agcagcaact aagtggatta cttttgtata
    11461 agaagataaa aatgataaaa cacttgaata agaaagaccc atcaaaaagg caattcctgt
    11521 aatgaaaagt gctttctttt caataaaact attgatattc catgagcgaa gagatatttt
    11581 ttgttcttca gataatggaa tattttttac aggaaagaca aggcaggcaa tcgtaacaaa
    11641 gaagaccagt acaattgaaa accagataat gaaacgaaaa tcagtcatat tcagcaaaat
    11701 cattcctaaa aatggcccaa cagcggcagc taaactagta cttaatccat aataattgat
    11761 gccttcccct tttttagatg cagggatata tgcagtaacg atagcatttg tagctgtaga
    11821 aactgtaccg tagccgaatc cattcaagaa tcgaactaaa tacaatgccc cgatagttgg
    11881 cgtataaaga tatgctaatg ttgaaaggag atagaagatc gcgccgcctc ttaaggtaaa
    11941 cttccgacca aatagttcta actgtttccc catgaataag cgtgccagca atgttccgat
    12001 gatatagatc ccagaagcaa aacctgcttg gctcaagctg gcgtgtaagg tgtcttgcgc
    12061 aatgactgcc atgatgacca tcaacagata ataaatcagg tagacaataa aattgatcaa
    12121 tgtgattcca ataaaatatt tattaaataa tttcttttcc atgtatgtta tacattcccc
    12181 ttaagctgaa tatgctttta atttttgtat ttgaacttct acatcgtgat aattggatgg
    12241 atagcgatca acaagttggc gaataaataa atctcttgta gacataaaca gatttaaaaa
    12301 ttcttcgcat tcgtttgttg gattctcatc cactgctttg atcaattcga tcgagttttg
    12361 atacgcatca tttaaaaaga ctaaagaagc tttcatatca aatcttgttt gatatgctgt
    12421 ttctaatcga ttaatgattg tgttcatcca gtaattcatg ttttttccct ctattcttaa
    12481 aaaattttac ttctgcagta taacgaataa aaaaaaggaa aaaaatcgca taagtgatgg
    12541 aaaaaggtac ttaacaaatg ttaatatcat tagtaaaata cctttggact ttaaaaagtt
    12601 ttaaagtact gtatgatctt tttagatgaa agtttggatg ggggagcatc attggatagt
    12661 agagtcttaa aggaatattt ggataaaaat gattttccga ttatacgcaa aggatatcat
    12721 aaatatttga cattcgaagg gatggaagat tcccatacgt atatcttgaa agagggaacg
    12781 gtaaaagcaa gtgttatttc aaatgatggg agagaattta accttcgcta tatcaaagat
    12841 cttgaaatcg tctctctttt aaaagacgaa tattcacaat tcatcgattc gccgtataat
    12901 attcgagttg aatcagaata tgcagagttt tatcgaatca atcgtgtgcg cttttggcaa
    12961 aatatcaacc aagacccaaa gctacaacac tatgtaaaag agtattaccg ctatcgtctg
    13021 ctttattcga tgaaaaaaat gcagcaaatg ctattaaacg gaaagctggg agctgtttgt
    13081 acgcaacttt acgaattata tgatttattc ggtgtgcgga cagaagaagg gatgctgatc
    13141 gatttttcga tcacgaatga agaaatcgcc aaattttgtg gcatctcaac tgctagcagt
    13201 gtaaacagaa ttttaaaaca actgaaaaaa gatggtatta tcaccgttaa agaccatcat
    13261 atcgttattt tgaatatgga cttgttagaa gacaatatta tcttttgatg aacaaagagg
    13321 ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    13381 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttaccaagag
    13441 gttgtgagcc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa cagcttttca
    13501 tgtttgatga atattgtgac ttgtgaccga ggagttggta ggagaacacc gtttaccaag
    13561 aggttgtgaa aaagctgttc tgcatcaagt aataagaaga gccaagtaaa aacagtctct
    13621 tatgttttta acttggctgg acttatcacc gtagatgtaa gcttttgaac gccgtttatt
    13681 tgcaacaaaa ggggctgtga cacgattttt gtcacagccc tttttttgcg aacattcatt
    13741 tagcaatttt tagaaacttc tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt
    13801 agtacttccg ccaccgccgc ttccaggagg tgggcttttg gggattcttc ttgtcgtgac
    13861 aaaagaattt gtcagacgat cggttttttc agttaatttg atactggatt tttctcggaa
    13921 tggatatttg tagatgcctg atttcaattg ataacgcgaa acagtcacaa aataaaagac
    13981 aagactaagg ataagagcca aaacaacagc aatgatgatt tctactgtcg tcaaaacctt
    14041 atagcgtgta attttgccag tttcttcgtc tacacgataa tgtcctcctg gaactccgtc
    14101 ttccacatat gttgctgatt tagacaggta agcttttgca gcattgtaat aggcagcatt
    14161 tgtcatctga tcataaacat catctaagat cgaatcgatt ctgctatcag tcaaataatc
    14221 gatcatatta ccagaagtcg aaatgtagat ttcacgctgt cccatatcta atagcaatac
    14281 tgagccattt tgatcatttc cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg
    14341 ttcttctgaa ttagatgtag tcgttacgat caagactcgt cctttgattt tttcattcag
    14401 cttttctgcc tgttgttcca aagattggat ttgatctgtt gtgaataacc cagcctcatc
    14461 atttaccgta gctgtatctg catgagtagc caccggccag ccaacactta ctagcaaaag
    14521 aaagaataaa aaaaactttt tcatcatatc aagtaacctc cgatcagaaa gaggatcgct
    14581 agcccagcga aaatcccaag agcggtcagt cccaattttt tctggctgat cggcaggaca
    14641 ccgctgacct ttcctgtttg tccattcatt gcgtagtagt agactttttt acttgagtca
    14701 ttgcttcgat aagtaacaag ccataccggc agtaatacgt aattatttac ttcgtttgtg
    14761 attgaagcag aagttcgcac accggtcaat gttgtatatc catttgcggt atcttttaac
    14821 agcttctctg aatagtcttt taattcagat tgaatctgtg atttgattgc ctcatattcg
    14881 atatctcttt tttctgcttg gaacccggct aaatactggc ttttaaaatc aattgcttta
    14941 tctaacggga aaggttgcac gccttcgacc atcttttgtt gcgtattttt tgataaggca
    15001 ttttttacta gttctttaaa agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt
    15061 tctgtgtatt cgatgtcacc tatccgccat atgcggataa ctgtaccatt cgcctgcatg
    15121 gcaccatcta cttcagcatc tgtagcccaa taagggaaat acacacctgt caacttatcg
    15181 atttgatcct tattgaaaaa agctttggga acaaaccatt ttttcttggt ccaagcgaga
    15241 aatctgttga ttgcttcttc tttctcgaca gcaaaaggaa gtactttatt tggcaagaat
    15301 tctccgctta aacgtcccga tagtacgacg ggattatggc agtagtaaca ataagtcgca
    15361 gcagttgtcg catcagtcac gatctctgca ccacagttgg gacaaagaaa aagatctatc
    15421 gctgcggtat tatctgcaga ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc
    15481 gctttgcgct cggcttcatt catatcggca agctgttctt gggctgtaaa agtaaaatct
    15541 tcggtaggtg aaggttcttc tttgctttct tcgccagcca tacgcgcttc tttttgcttt
    15601 tgctcatatt ggctgacttc ttcttctgta tagatattca ggcagtattc gcaatgaaat
    15661 ttttggtctt tgggatcaaa agtcaacgga ccgccgcaat taggacattt atgtgtaaga
    15721 acatccatca gctgcacacc tttctaaata aattaattaa cggggattcc tttaaacggt
    15781 ttaccacagt ttggacagaa tttagggatg ccgttggaaa gatccacgat ctcattgcat
    15841 tcactgcatc gcatttgtag cttaggctga acgttttcta caggtttggc agctccgcag
    15901 ttggaacaga attttccgga attttctgtc ccgcaaactg gacatgtcca agtatccgca
    15961 tttccttgtg cagtttgttg attttgtttt tctgcttgtt gtttgatttg ttcttggtta
    16021 ttttgagctg cttgagaaag ataagaacca tttgcattca ttcccatacc tacacccata
    16081 aagccggtca ttgctcctgc ttcatttttt ccggctgctt ccataccacg tgcaatggaa
    16141 ccttgcacgt atccttcacg tacgctagga tcacctagca tcgccccttc gttacgcata
    16201 ttgatcagtt tcacagaatc atcggtgtaa gaaatgctgg ctacagccac agaaacaatc
    16261 tccatcccac gtaattcacg ccaagaatca tccaatgcag tatccatgta tttgcttaat
    16321 tccaaactct tagatggtac ataagaaatc cgttgaccat ctgcagacat ctgattgatt
    16381 gctgattgta gcgctgttag gaattcagct aaatactgct cattgatatc attgatttcc
    16441 acttgtgttt tgtttttagg gattgcgttt gtataaaata aaatcgggtc aacgacatga
    16501 atagaataag tgccatgtgc ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag
    16561 ttcaacggcg cgcttgtacc gaatttgatt ccctttattt cttggagatt gatataaaaa
    16621 acttgttgtt tctgcggtgt gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt
    16681 gcttctttca gtgttccatt gaacatggaa ggagctgcgt catttttaat ggtgtaataa
    16741 ccttcctcag cggtataatc gatgattttt ccaccatcga ccaaaagcat catcatgttt
    16801 ggatagacat ggacgactgt tccgtctgtt aaaacatctt ctgttccttt acgattggag
    16861 ccacgtttgt catctttgcg gactttgaca ccttttgtca tgaccgttgt atcactcata
    16921 ttgtctggtt cgatgacttc tagccattga tctgccagac cgccgccaac catacttgtt
    16981 gctgctttga taagacccat aaaaaattcc tcctctgatt cagctagtgc caaacaatta
    17041 ttgtttattt attataccat aggaagatgt gaaacttctc ggactagaga cggaattgaa
    17101 atggaaaatt gcgtattgtt taaatacctg aaaaagccga gaaggatagt agttttttta
    17161 cctaatatca cgtataataa gagatagtga attttttaaa aaagggggat ccttatggca
    17221 tatcaagcac tttatcgagt ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa
    17281 gctgtgacac agacactaaa aaatgctatt gtttcccaca aaacctcaca tgcctacttg
    17341 tttacaggac ccagaggaac cggaaagacg agtgcagcaa agattttcgc aaaagcaatc
    17401 aactgtccga acagtaaaga cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt
    17461 actgctggta cgcaagaaga tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa
    17521 gaaatccgat ttatccgtga tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg
    17581 tacattattg atgaggtaca tatgctttca acaggagctt tcaatgcctt gttgaaaaca
    17641 ttggaagagc caaaagaaag tgtcatattc atcttagcaa cgaccgaacc tcacaaaata
    17701 ccagctacga tcatttcacg gactcagcga tttgatttta aacggatcaa tacggcagat
    17761 attgtcgaac atcttgaatt tatcttgaaa gaatcgacta tcagctatga accagctgca
    17821 ttgaatgtga ttgcccgttc tgcagaaggc gggatgcggg atgcattgag tattcttgat
    17881 caggcaatct cgtttagtga aggaacaatt actttagatg atgccatgca agtgacaggt
    17941 agcttaactg acgaaatgat ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca
    18001 gaagcattgg aaacattgaa ccaaattctc gcagccggca aagaatcacg tcgctttcta
    18061 gaggatatgt tacaatactg ccgtgacttg ctgatgtatc agcaagcacc taacctagtt
    18121 gaggaacaat cgagtacgat cagaagtaca tttacagaat tggcacaaac tgtttcagcg
    18181 gatcagctgt tttcaatgat aaagattttg agcgatactc aaaatgaaat tcgttttacc
    18241 aatagtgccg ctatttattt ggaagtcgct acagtcaaac ttgcacagct accgtctgct
    18301 tctgttcctt cagcagatgt agcatttgca gatgatcaga tagtaaaaca gctacaacaa
    18361 gagattaagg aactaagaaa cgaaatcaag gaactacgca gtaatacagg aattgagaat
    18421 acaaaagtac agccggtaca gcaaacaaat cataaaaaac agacaagtac ctatcgtgta
    18481 ccgacggaac gagtatataa agttctgcgg gaagcgacaa gacagcatct gaaccaagta
    18541 aaagaagtat gggaagactt gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa
    18601 gcaagcgaac cagttgcagc tggacctaca ggactagtga ttgcgtttga ttatgagatt
    18661 gtttgtcaaa gagcagcaaa tgacgaagaa ttgcagcttt cggtacacaa caacttaagc
    18721 cgaatgatca aagattatgc gcctgatcct gtttatatta caagagaaag ctggccagaa
    18781 cttcgaagaa attatttgac gcaggcacaa gaagagggaa cagaaaactc ttttgcttct
    18841 gaagactcag aaggagaaat cgagttgctt cctcaagaac aagaagtacc agaagaaaca
    18901 cttgtagtaa aagcagagga actgtttggc tccgaagctg ttacagtggt agatgattaa
    18961 taaaaaggag tagataatta tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca
    19021 agtacaaaaa atgcaaaaag aaatggcaga agcacaagag ctgctaaata aaacagagtt
    19081 tacaggtaaa gccaccaatg aattggtcaa agcggttttt acaggcgatc gtcgcatgaa
    19141 agatatccaa atcaatgaag aagtcgttga tcctgatgat atcgagatgc ttcaggatct
    19201 tgtcgtaatg gcagtcaatg atgcgatcgc acaaattgat gtggaaactg agaaaaaatt
    19261 agggaaatac gcaaaaggtt tgccaggatt ttagttttaa actgaaggag taatgaatat
    19321 gcaatatcca gaaccaattg cgaaactgat cgacagctac atgaaacttc caggaatcgg
    19381 tcagaaaaca gcgacaagac tcgcctttta tacgatcgat atgaaagatg aagtagtaaa
    19441 tgaatttgct aaatctttgt taagtgtaaa acgcgatttg catttttgta gtatctgcgg
    19501 aaacatcaca gaagaagatc cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt
    19561 tatcctagtg gtcgaagagc cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca
    19621 cggactttat catgttctcc atggtgtgtt atcaccgatg gaagggacag gtccagaaga
    19681 catcaatatt tcttctctgt tgcagcggct gcatgatgca gatgtaaaag aagtgattat
    19741 cgcaacaaat gcaacaacag agggagaagc aacagctatg tatctctcac gtctgatcaa
    19801 acccgcaggt atcaaagtga ctcgtctagc acatggtctg tctgtaggct ctgatatcga
    19861 gtatgcagat gaagttactt tattgaaagc tgtcgaagga agacgtgagt tataaatttc
    19921 taattaagtg ttttttcaga tacaatataa aagcagcaca agagatgggg gcaacaatgt
    19981 gaacggacta tttattacaa tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa
    20041 tgaactttac cctcttttga aagaagtagc aaaaacagaa atcattcaaa caagagaacc
    20101 aggaggcatt ccgattgctg aagagatccg agcagtgatc cttgatccaa aaaatgatcg
    20161 gatggatgaa cgtacggaag cactgcttta tgcagcagca agaagacaac atctagtcga
    20221 aaaggttctg cctgctctgg caaaagggaa aatcgtttta tgcgatagat ttgtcgacag
    20281 ttctcttgct tatcaaggtg caggccgacg catcggcgtt accgagattg ctcggttgaa
    20341 tgagtttgct acagaaggta ctacgccgga ttttaccctt tatcttgacg tagattcaga
    20401 caccggatta agaagaatca agaaaaacag acaaaatcaa atcgaccgtt tagattcgga
    20461 aggtttggaa ttccaccagc gtgtacgtca tgcttactta aagctggcag aagagaatcc
    20521 ggaaagaatc cacaaagttg atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta
    20581 tcatacaatt attgaacagt atccgcaatt ttttgaaaac taggaaggtg aacgtttatg
    20641 aaaattattt tagcgattat ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt
    20701 gatgcaaata tccgtgccac aaaactatca tcgacaggcg gcttccttaa ggctggaaac
    20761 agtacgttta tcgtgggtat tgaagatgac cgtgtagaag aagcattgga aatcatcaaa
    20821 aaaacgtgtg aatcaagaaa gcaatttgta tctacacctg ttacactgga tatttcgatg
    20881 gatgggggcg tcccttatcc agtagaagta gaagtaggcg gtgccacagt cttcgtctta
    20941 cctgttgaag gattccatca attttaggag gagctgagct atgaatcaag aaataaactt
    21001 ggctcaaatg caaccgatcg tgtatcagca gcttcaacgg agttttgagc atgggcgtat
    21061 cgctcatgct tatctttttg aaggggagaa aggaacagga aaacatgaag taggtatatg
    21121 gcttgcacag cacctgttct gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa
    21181 caattgtcag cggatcgaaa agaaagaaca tccagatgtt ttggttattg aaccggaagg
    21241 acaaaccatc aaagtcgatc agatcagacg tttgcagacg gaattcagtc gcagcggtta
    21301 cgaatcaaga aaaaaagttt ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc
    21361 aaacagtttg ttgaaattct tggaagagcc ccctggagat tttttagcgg tgttagaaac
    21421 agatgcagta ggaagaattc tccctactat tcaatctcgt tgtcaagttc tgcattttca
    21481 agaactttcg aaagaagcac tgatccatcg attgcaagaa gagcagattc cattggaaaa
    21541 agcaaaactt ttggctttct taacaaacag tttaggaaaa gcagttgaaa tctctcaaga
    21601 tgaatggttt aatgatgcta aggacttgat ttatcaatgg ttcgtttact tgcaaaagca
    21661 agatactcaa gcctttatct atgtccaaaa aaaattggta aaatcattta aagaaaaaac
    21721 acaacagttt acaggattat ctgtcttgat gttttattat caggaagcac agcgtcaagc
    21781 agtagcaaac ggcgaatatg gcaaagtgaa acagataaac cagataattg aacggatatt
    21841 gatcgcagag cagaaactac gcagcaatgt cagttttcag gctgttgcgg aacagttcgt
    21901 tttgcatacg attttttagc ttgccgcaaa ggatgatgat caaggaggat ggaaatggta
    21961 gaagtagtag gtgtccgata tagagatgca ggtcatatct attattttgc accaggagac
    22021 tctgaatatg tttatagtga caaggtatta gtcgaatctc agcaatcctt acatattgct
    22081 actgttgtcc ttcctaaaaa agagattgat cccaatgacc ttcctgaagc tatccgtccg
    22141 atcttgcata aagcgtcaca agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct
    22201 tatgatgcat tgaaaatcgc aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg
    22261 gtcaaagccg aatacacatt tgatcgaaat aaattgattt tttctttcac agcggacggg
    22321 cgaattgact ttagagaatt ggtaaaagat ctggccgctg tatttcgtac tcgtattgag
    22381 cttcgacaaa ttggtgtccg cgatgaagca aagataatag gcggaattgg tccatgtggg
    22441 cgtccattat gctgttcttc ttttttaggc gatttcgtgc ctgtatcaat caaaatggca
    22501 aaagatcaaa gtttatcttt gaatccaaca aaaatttccg gtttatgcgg acgcttgatg
    22561 tgctgtctgc aatatgagag cgaagaatac gagacagcga aaaaagaact ccctgacttt
    22621 ggaaaagtaa ttgctacacc tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc
    22681 cgtatcgtta gtattcggct gactggacgg gaaaatcctg tcgaatatga atgggatgaa
    22741 ttgaaagaat ttatccaagt aggtgagatg aatggataag agatcattat acgacggatt
    22801 gaatcaatta gaatcagagt tgagaaatac attaacagaa cttgttgaaa tgaagcatgc
    22861 tttgcatgag atcgtagaaa aaaatacaac tttggagatg gaaaatcaac gtctgcggga
    22921 acacctgcgg gaattgaacc aagttgcaca agctccagct gaaaatgtga agcaggaact
    22981 ttctaagtct cgtatgaatc ttgaaaaaat ttatgaagaa ggattccatg tctgctacga
    23041 tttatatgga tcaagaaggg acaatgacga gccttgtgcg ttttgtctag aagtgatcta
    23101 tcgcgaaagc ggtcgatgac caataacaag aggagactgg agcggagaaa tactgctcca
    23161 gtctttttat taaggagatt gtgacagatg tataatcaaa aaagtttcaa gggaaatccg
    23221 gtatatgggg atttatatct tgtaccgacg ccaattggaa atttagaaga tatgagctac
    23281 agaagtgttc gtatgctgca agaatccgat cttatcgcaa gtgaggatac gcgtaataca
    23341 caaaagttat tgaatcattt tgaaatccat acaccccaaa aaagtttaca tgaacataat
    23401 tataaagaac gtgtccctca attgattgct ttgttaaaag aaggaaaaac gattgctcaa
    23461 gtcagtgacg caggaatgcc ttccatcagc gatcctggac atgagctagt tttagcctgc
    23521 ataaaagaag ggatttctgt gatctctatc ccaggaccta cagctgggat gacggctttg
    23581 attgcttcag ggctagtgcc tcaaccattt ttattttatg gcttccttcc acgtaagaaa
    23641 aaagaacaga aagaaacatt gaaaaagcta aaagaacaga cagttacgct gattttttat
    23701 gagtcacctt atcggatcac tgctacattg acgaattttt tagaaatttt tggaaatcgc
    23761 caagtcgttt tgtgtcggga attgacaaag gtccatgaag aataccttag aggaagtacg
    23821 gaagagctac tcgcttatat cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta
    23881 gaagggaatt tgttcgaaat gccagtgacg gaagttgatg aagagttggg aacgttaaag
    23941 gaacaagtag aagcaaagat cgcttcaggc gaaaaaccaa atgctgctat caaagcagta
    24001 gcgctgaaaa atgggctgaa aaaacaagaa gtctaccgtc agtaccatga attagactga
    24061 gatagtataa gttgtctata atgaaagact aatgaggaaa tcagctacta acttttcaaa
    24121 tctcttgaac gcgaacgaat gttcttgtat aatgaataag aggtgaggat gatggagctg
    24181 caatttcctt tacataatga tacatcaaga aaaatcattc atattgatat ggatgctttt
    24241 ttcgcttcgg ttgaagaacg agatcatcct gagtttcgcg gacatccatt agtaattgcc
    24301 agacacccca gtgacactgg tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga
    24361 aaattcggaa ttcattcagc aatgagtgca caaaaagcgt atgagctttg tccgcaagcc
    24421 atctttaagc ctggcgacca tcataaatac agcgatattt ccaaacaagt acgggaagta
    24481 ttctatcgtt atacagatat tgtggaacca gttagtatcg atgaagcgta tcttgatgtg
    24541 accgaaaata aaatcaattg caagtcagcc atcaaaatcg cccgaatgat ccaagcagat
    24601 atttgggaga gtgtccagct aacatgttct gcgggagtaa gttacaataa gtttttggcc
    24661 aaattagcat cagactatca aaaacctaga ggaatcacgg tagttgcccc tcaagacgct
    24721 gtgccatttt taaaagcttt gccaattgaa aaatttcatg gggtagggaa gaaaactgta
    24781 cctagaatgc atgagctagg gatttataca ggaaaagacc tatatgaatg cacagaaatg
    24841 atgttgattc gaaattttgg aaaaatgggc tattctcttt atcgtaaagt cagaggaatc
    24901 catgattctc cagtaaacgt tacaagagag cgaaaatctg tgggaaaaga gcatacttat
    24961 gggcagccgt tgcaaacgga agaagcagtg ctgactcagt tgcgccaact agctgaaaaa
    25021 gtagaagaag cactgagaag agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg
    25081 tacacggact attctactgt gaccaaaaga gtgacattgc cagaatatat ctataaaaaa
    25141 gaagcactct tttatcaagc aagccttata tgggaggaaa tcttgggagt agaaaaagga
    25201 atccgattgt tagggattac tttgacgaat ctggacccga tgacttatga aaacatcgtc
    25261 cttccattat gggaaaatca agaaatataa aaacagcttt gagagttgaa accggaaatc
    25321 cgttttctct cccaaagctg tttttatatc aagtcttgct ttttgatttg ttcataggtt
    25381 tctgttgcat ccgtacattt ttcccaaatc acaacttgat tggattctaa agatttttgg
    25441 acgtcaatga tccgctggtt gctacttccg cgaaattgta gttttaggtc acgtttgctt
    25501 aattcaaagc gtccgtcaac aaggatatcg atttgcgaaa ggagttctaa tttatcttct
    25561 gaatcttgta gcaattcatc aaaggcatag cctgtccatg accagatatc ttttgtcgta
    25621 ccgaactctt ttcggatacg tcgtacgact tgcagacaaa catctgtatt caaaaaaggt
    25681 tcgccaccaa gtaaagttaa tccttgcacg taatcgttgc gcaagtcagc gatgatttgt
    25741 tcttctaatt caggtgtgaa tggtttgcca tatcggaaat tttgtgcagc tttgttgaag
    25801 cagccttcgc aggcaaataa gcagccacta acgtaaagac tgttgcggac accttcacca
    25861 tcgacaaaat taaacggttt gtagtcagca atatagtttt ggctgtagtc ttttgctaac
    25921 cattcttttg gttttggatt tctcataaaa aaacttcttt catttaaggt tcaaagatat
    25981 agctattgaa gataaaatct actcgaccac ataaataaga acaaactgac ctaaatccat
    26041 tgttcaagaa agcttgttct tattttgata gagggcgttc aaagatacaa agccatcgaa
    26101 agagggtggt gaaaaaagac ttgtcatagc ctcttattct gaataaacgg tgttcaaaag
    26161 cttgcatctg cagtaataag cccaactaaa cgaaaaatat gaggaactat ttttgtttag
    26221 ctattcttat tacttgatgc aggacagctt tttcacaacc tcatttcatg tgtttcacac
    26281 gtgaggaaat ttctttatgg cggccatgta ccatcggacg agcttgcgga tttcctaaat
    26341 agccacaagt acgtttgact acatcacatg tttttggatc atgatttcca cattctggac
    26401 attcgaaccc tcgttctgtc ggtttgaaat caccttcgaa accacattga tagcaatgat
    26461 cgattggcgt attcgtacca agatacccga ttttgtcata tgcaaaatcc catacagctt
    26521 ctaaagcttt cggattttgt tggagcatag ggtattcaca gtagtggatg aatccacctg
    26581 aacaatattt tggataatcc ttctcaaatt ccaatttttc aaatggcgtt gggtttttac
    26641 gcacatcata gtggaaactg tttgtataat attctttatc tgtgatattt tcgatgatgc
    26701 cgaatttttc tgtatccaaa cggcagaaac ggtcagttag actttcactt ggagttgaat
    26761 agacactgaa atgataacca tattcatccc cccatgcatc tgcattagct tttaattctt
    26821 tgacgatatc aagtgtgaaa gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga
    26881 aagctgaagc aacttcatac aatccaatgt agcctaacga tacagtagct cgacggtttt
    26941 tgaataattc attgacagaa tcggtacgtg aaagacgttt cccgaaagaa ccatacatat
    27001 ataaaattgg cgcatttgct ggaatggctt ctttacaacg ttctacacga tagagaagcg
    27061 catctttcat gatttctaag cggtcagaca gtaactgcca gaatttattc tgatctcctt
    27121 ttgattcgat cgcgattctt ggcaaattca atgtaacaac acctaaattc attcgtccga
    27181 catttacttc ttgaccattt tcgtctttcc acccttgcaa gaatgaacga cagcccatag
    27241 gtactttgaa acttccagtt aattcaacga ttttatcgta attcagaata tcaggataca
    27301 ttcttttcgt tgcacattcc aatgctagtt gtttgatgtc gtaattagga tcttcagggt
    27361 ttaagttcac accttttttc aaagtgaaga tcagtttagg gaagatagct gtccgctttt
    27421 cactccctaa tccattgatt cgaatctgta aaattgcttt ttgaatttct ctttcaaacc
    27481 agttttctcc tagtccaaat cctaatgaag tgaaaggagt ttgtccattt gaagtaaata
    27541 aggtattgat ttcatattcc aaactttgca tagcatcgaa gatatctttt ttggtttttg
    27601 cttttgcata ttctttttgg cgttctggcg aatcgatcca ttcttccgcg tcttttagat
    27661 gttttttata gttcaattct gcaaaaggag caagcaattc atctgttcgg tcggcagaac
    27721 agccaccata ttgacttgac gctacattgg caataatctg tgagatttgt gctgtggcgg
    27781 tctggataga ttttggcgat tcgacttcgg cattgccgat tttgaaccca ttgttcagca
    27841 tgcctttgaa atcaatcaag cagcaattcg tcatcggtgt ataaggatga taatccaaat
    27901 catggtaatg gatatcgcct ttttgatgcg catttgctat atgtggtggc aacattttca
    27961 gtccgattga ttttccaacg atcccggctg tcagatctcg ctgtgtattg aaaacattac
    28021 tgtctttatt tgcgttttcg ttcacgacag tttggtcctt gttgattagc ttttcaatcg
    28081 aaaagttgat atctgttgct ttactgcgag caaaatctcg tttggtacga taattgatgt
    28141 actcttctgc taattcatat tcgttttttt ctagtaatat atgttccacg atgttttgaa
    28201 tctcatagat tttgacattt tcagcaaacc ggcttttgat ttcttggaca accaagtctg
    28261 caatcatttg aattttttca taagtaagtg gtgataatga gccgtgaatt ttcttttctg
    28321 ctttgatcag tgcttcattg atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt
    28381 tgatgacttt catctgggat gtctctggct gctgagtaac gacagttttt tcttcataat
    28441 ttactttcat tatcttcaac tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg
    28501 ggaaggagca actaaatatc ggctatatat agtattcaat gtgctgtata attggatttc
    28561 tatactatat gtagtgtata ttttattgtg aaaaaaggtg aaaacttgta gctggtggat
    28621 ttgttttttg catatttaat tttgctgaat aaaataattg aataaaattt tttattcagt
    28681 ccaagtaaaa gtgacaaaaa cataagagca aattattcgt agatatgcta ctatgcaaat
    28741 aaatagaaat gaaaaatgaa agaggaggag aagaattgga aaagatgatc gaagtgaaac
    28801 atctaagtaa aagctacggc agcaaacttt ctcccgtcaa agccttagat aatatctcat
    28861 tcacagtaga agaaggcgag tttgttggga ttatgggccc aagtggtgca ggaaagacaa
    28921 cgctgatgaa tatcttgtcc acgatcaatt tgcctacgat gggcagtgtc tcgatccaag
    28981 gaaaagaaat cacaaaaatg aaaaacagtg agctcagtga ctttcgtcga aaaaaactag
    29041 gattcatttt tcaagagttc aatttgatcg ataccctaaa tgcaaaagac aatatcctat
    29101 tgcccctagc agttgaacgg atgactaaag aggaaatgga aaagagagta agacatgtag
    29161 ctcaattgtt gaatatagaa gaactgctca aacggtatcc cgatgaactt tctgtcggac
    29221 agagacaacg aattgcagct gctagagcac ttgttgttca gccacaagtg atttttgcag
    29281 atgaaccaac tggctctttg gactcgaagt cggctacaga attgttgaac tacttgaaga
    29341 ccatgaatca aaaagaaaaa gcaacgatac tgttagtcac ccatgatcca tatacagcaa
    29401 gctactgcga tagaatttta tttattaaag atggtgtgat tttttctgaa gttgtcagaa
    29461 gagggacaag aagagagttc tttgagaaag taatagacat gcaggcgacg attggcggag
    29521 gaggaaaaat gaatgctgta taaactagcc gttaaaagtt tttttcggca gtcacgaggc
    29581 tatcttgttt attttttcag tttgacttta tccgtaatga tctattattc cttcagcgca
    29641 atgacctacg atcaatcttt agtcagaaga gcaagtcaag atacttcgat agatggtgga
    29701 ttgagttttg gtggatttat cattactgtt gttttgcttt ttttcgtatt cagtgccaac
    29761 cgatttttct tgaatagaag gcaaaaggaa ataggcatct atcagctttt tggtatgaat
    29821 aaactgcaga tatcaggtat atatgtgtta gaaatcatga taattggatt gttcgcctgt
    29881 atttctggga tattactcgg tattattttt tccaaactct tttcaatgat tttggttcga
    29941 atgatggata tggatcttac tagtccattt tttatctcca ttccatcagt agtagatact
    30001 ttatttgctt ttttcatcat ccttttagca gtatctgtat attcaatatg gaaaatatgg
    30061 cgctatccaa tgattcgaag ttttggagaa aaagaacaag cagatagttc aacaatgcgc
    30121 ttcaggacaa ggcaccgatt actcgcagtc atcgggttac tacttttaac gagcggatat
    30181 tttggagcta gtcattttag ggaaatcact tcatggatga tcagttatca ttatcttgat
    30241 acattagttt tcttttttcc ttttttaatt tttttcgttt gtgtggtcgg tacttatttg
    30301 tttttctgtt atacatttcg gttatttttg atcatattga gtaaatggaa gatttattac
    30361 tatggtatca attttttgat aatgggtaac acgcaagttc atttgctcaa aagttggcgt
    30421 acaaattctc tgattacagt cattctcgga atttctttaa tgacaattgg cggcacggct
    30481 agtatctcta ctattttatc ctaccaggag aaagtttcct ccccgatgga ctatcagttt
    30541 gatgttgaga cagagaaaaa aatcaagccg attctagcag aggaagggca gaagataact
    30601 aaagaaatca ccttgcatta taaagtcatt gggagtatct atagtcaaaa tctcgaagaa
    30661 cctatgtttc aaactgctaa tctgatcacg gaaacacaat atcaagcctt cagaaaagtt
    30721 aatccagatt tgccgaacat ttctttgaaa tcacctggtc atactgtttt gcttgatcaa
    30781 atgcaaactc tttttcggaa tatttcatta tatggatcta gtattggact gccagataat
    30841 caagaattga agattcaaca aatgcttcca agtgtattag gtgacgaaac aatggtatat
    30901 ctaagtcctg tactgattgt agatgatacc gtttttgaaa aagcaaaagg tgtggattat
    30961 caagtagtca atgtagatgt atcaggtgga aataatgaaa aaatagatga acgactgagc
    31021 aatgacctgt cgataaagtg gctcaatgct gtttattatt cttatgatat cgtaaatgga
    31081 agtctacaag gagaaattag cacaaagaaa cttccggatg accaaatgaa ggaagcacaa
    31141 tttagccaag aaagctctcg tttgaatttc tcttccagct attcgaaatt gcgtgcagtt
    31201 aatcggcgaa tcggtatcaa tatttttgtc acactatttg tcgggatgat cgtgattatt
    31261 gctactggta gtattttgac agtccggcaa ttagcagaag cagaggaaga aaaagaaaat
    31321 tatcacctat tgactaagct ggggattcca caaagaagga tcagacggat gatcttcgag
    31381 caaaacgcac ttaccttctt tccacccatg atattaggga tcactcacgc agtatttgca
    31441 atcaatgtct tcactcagta tataaaaact gccgattact ggttggctta tcttgtgagt
    31501 ggcttattga tccttatcta tcttttgctt tatgtcatta cgagtcagtt atattgtcgg
    31561 attatcgagg aatgatatac ggaataagac aagataataa atagaactaa attttgaata
    31621 ccatttattt ggaataagag gttgtgacac gatttttgtc acaacctctt tattttttat
    31681 attttttaaa agtacgagaa attataaaga aaaaagcagt gatttgatgt gctattttta
    31741 taaaagctat gtgacaaaac tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt
    31801 tctttttgtc aaaattagtt acactttgtg tgaggttata tgaggaggga atccaatgaa
    31861 caaatggcgt tctaatccaa aattgaatta ttggggaaaa ttcatcattc gaacaatttt
    31921 ttatagtgca gtactgctgt tcctgattta tctgtatcac tataaaaata ttcaaggagg
    31981 aacttttatc tataacgaat tttaggagga agagtatgga aatcaaaacg attattgaag
    32041 caatcgatga ttggggaata aatgaacctg atcgagttgc ttatcaggcg gatgagacgc
    32101 atacttttgg tgaattgaaa caagcatcag acgcacttgc ttattattta gaaaaaaagg
    32161 tagaaggcga tggaccaatt gtcgtttttg gcaatttgga gtttgaaatg attgtctcat
    32221 ttctaggggt tgtcaaatct ggccatgcct atttgccgat tgaagagcat acacccaagg
    32281 agcgtatcct atcgatcttt cgggtggcta aaccatctat ggtcatcagt atcggggact
    32341 ggatagaaga tattccttcg gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga
    32401 agataccagt aggattttat cccagaaata gcgtaaaagg tacagaaaat ttttatatta
    32461 tttttacttc tggtactact ggagaaccaa aaggcgtaca aatcagtcat gataatctag
    32521 tcagctttgt tcaatggacg atggaagact ttgggatcaa gcctggtatg catttcttag
    32581 cccaagcgcc attttctttt gatttatcgg tattttctat ttatcctgca ttagtttctg
    32641 gcggaatgct gaagccgtta aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga
    32701 cattgccagc cttgaagttg aatgtgtggg tttctactcc atcatttatg gatatttgct
    32761 tgatggaacc tacattcaat ggagaaaatg tgccggaatt ggacatgttt ttattttgcg
    32821 gagaagaatt gacgaaaaaa actgcagaaa gtctacttga tcgattccca aatgcacgga
    32881 tatataatac ttacggtcca acagaagcaa ctgtagctat ttcaagtatt cagattgacc
    32941 agcatgtact agatagttat gaccgactac cgattggcta cgtaaaaaaa gacacgaaag
    33001 tttctattgt ccaagaaggt aaacctgttt caaaaggaga aacgggtgaa atcattattg
    33061 caggaccaag cgtatcaaaa ggttacctga acaatcccga aaagacagct gctgcgttct
    33121 tttgtcaaaa tgacttagca agttaccata caggggatgc aggtagacta gatgaatatg
    33181 ggcttttatt ttatgaagga cgtatggatc agcaggtcaa attacatgga taccggattg
    33241 aactcgggga tatcgaacat tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg
    33301 taccaaaata ccaaggaaca aaagtacagc agctggttgc ttttgtcgta ttagaaaaca
    33361 aaacgaaaca gccggatttt cagttgacta aatctatcaa agaacaatta ctagaagttg
    33421 taatggatta tatgattcct cagaagttcc attatgtcga tctgctgcct cagacagtta
    33481 atggaaaaat cgatcgaaaa aaattaacag ctgaggtgaa tcctgaatga tgttacctca
    33541 tatgatccct tatgcagagc cgttttattt ttttctttta gccattgcgc ttgtaccgat
    33601 cgttctatct ttattgatat gggaaaaaag attaccagtt tatcaatcag cagtgacact
    33661 tttcttttta tttgtcagct ttggaggtaa ttattggcaa cagggagcgg cgctgattgg
    33721 gtatgttttg tggcagacga tacttgtatg gggctatttt gtttatcggc aaaggaaaaa
    33781 tcagacagga tggttttttg ccgccgttat tttagcgata attccactgt ttttaacgaa
    33841 agtgactcct ttttttgaac atggaaaagc atcgttactt ggttttttag gaatctctta
    33901 tttaaccttt aaatctgtac aaatgatcat ggaaataaga gatggcatga tcaaaacata
    33961 taagccattt cgttatatcc agtttctgct gttctttcca acaatctctt ctggaccaat
    34021 cgatcgctat aggagatttg aaaaagatca gctccatccg cctgttcgtg ataaatatgt
    34081 tgatcttttg ggaaaaggcg tccaaaatat ttttgtcggt tttttatata aattcattat
    34141 cggttattat tttggtcaag tactgcttcc tgtagtggga cgcattgcct tacagcatgg
    34201 tggtatttca tgggcattag taggttatat gtacgtgtac agtatgtatt tgttttttga
    34261 ttttgctggc tatagcttat ttgcagtcgg tacaagctac atgatgggat atcagacacc
    34321 aatcaatttc aataaacctt ttttatcatg gaatatcaaa gaattctgga atcgttggca
    34381 tatgacgctg tccttttggt tccgtgatta tgtatatatg cgattgatgt tcttcatgat
    34441 gaagaaaaaa ttttttaaaa gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt
    34501 tcttctaatg ggtatatggc atggattgac gtggtactac atcgtatatg gactgtatca
    34561 tgcttgtctg atctgtctga cagatgcatg gatccgattt aagaagaagc ataaagagca
    34621 gttgccttct aacaaattta cgcatggctt agccgtattt ttgacatttc aagcagtatg
    34681 ttttagcttc ctgatttttt caggattttt agataaactt ttttttagct aacgaatagt
    34741 gacatattaa tttatataac aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc
    34801 ttagaagaaa taacaggaac agatgaagta agaaaaaatc gtgatattga tctatttgat
    34861 gaagggttgc ttgattcttt agcatctgtt caattattag tcgagctaga cggagagctg
    34921 gatatccaag tccctgtatc tgaattcgag cgagaggatt ggagcacacc gaataaaatc
    34981 attcaacaag caactgcatt gaagggttga gtatgaaaaa gaaacttttt gctattttcg
    35041 gcccggttgt tttagctgtt ggcttactgt ttgtgttttt tacctcctcc tatcaagtag
    35101 atatctctaa tccagaaacg attagacgtg cctcttcttc catgtcagtg aacgtgttga
    35161 aaggagatgc gataaaaaat tatgcattat ctgaaaaaca atatattcct ttttttggct
    35221 caagtgaatt aagcagaatc agtccatttc atccgtctgt tctggcagaa aaataccagc
    35281 gaaactatcg tccatttttg ctgggagcac ccgggacaca atcgttaagc caatatatga
    35341 tgatgcgttc tgcaggagat gctatgaaaa acaagaaagt cgtctttatt atttctcctc
    35401 agtggttcgt taaaaatggt gtaaaaactg attactttaa tacgtattat tctgaacttc
    35461 aaacatacga ctggttgttt tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta
    35521 gaagattatt aactttctca aaagtaaaag agaatgatac gttgacggcg attctgcaaa
    35581 cgatcaaaaa agggaagcta cctttacctg aatctttgaa tcagctgcgc agccaatgga
    35641 acatgctgaa gagagaggat gaagtattca gcaatattgg gttgaaagat cgacagcaaa
    35701 aaatcgatca tgagtcaaaa cgattaccaa aacaatacca agaaacagaa ttatccattt
    35761 tagcaaatca aattggagaa cgagaaacga cgaataatcc attcgggttg aaaaacgatt
    35821 tttatacgca tcgtatccga gcccacgagc cagagctcaa acagtcacaa aaaaattggg
    35881 attatcgttt ttcgccagaa ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa
    35941 accataacga agtattgttc atcatcccac cggttaacga gaaatggagc gattataccg
    36001 gactatctca agaaatgtta caaggatttg ccaaaaagat caaatttcaa ttaaatagcc
    36061 aaggattcaa tcgaatagct gattttgtca atcaagcggg taccaactat tttatggaag
    36121 acacgatcca tctaggctgg aaaggctggt tggctgcaga tcaacagatc agaccgtttt
    36181 tagaagaaaa tcatattact gctagtaagt atcatcttga cgatgctttt tttagcaaat
    36241 cttggcaaca tcaaatacca gataaactac aattaaaata aagaaaacag gcgagtgttg
    36301 ctcgcttgtt ttctttattt ttgaatattc tgaataaaag ataaaaatac caagtttgcc
    36361 tgaacaaaaa tcataagcaa tggtataatc acaatatgaa tatagaaatg ggggaaaaca
    36421 tttggataca aaagaagaac agctacttat tgatttaact tctgcaaaag gagtacctgg
    36481 aaatgaagaa gaggtaagag aagtattcag agaatatgcc aaaccattcg ctgatgatat
    36541 tttttatgac ggtttaggca gtgtgattgc caaacatggt gctggcggag gccccaaggt
    36601 ctttatttct ggacatatgg acgaagttgg ttttatggta acaaaaataa ccgaaaaagg
    36661 attcttggaa ttccaaactc ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt
    36721 tgaaatcaaa acacgtgaag ggaaaatcgt ccacggtgtg attggttcca aaccaccaca
    36781 tgttttaaca cctcaagtaa gaaataaacc ttatgaaata aaggatatgt tcattgatat
    36841 tggcgcatca agccaagaag aagccaaaga gtggggcata cgtcctggag atatggtcac
    36901 tccatatatt gaatacaaac gaatgaatgg cagtaaatat ttactggcaa aagcatggga
    36961 taatcgtatc gggacagctg tttctctgcg agtattagag aatctatcta aagaagcaca
    37021 cccaaatgtt ttattcgcag gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg
    37081 aacaagtaca caccttgtaa atcccgatat tgcttttgct ttagatactg gtacagcagg
    37141 agacactcca ggaatgacac caaaagaagc agattcgatt ttaggaaaag gcccacagat
    37201 tttgattttt gatgcatcta tgatcccgca taaaaaatta ttgaattttg ttattgatgt
    37261 agcagaggaa atggatattc cattccaata tacagtgatt tctggcggag gaactgatgc
    37321 aggacaaatg catttgacaa gagatggtgt tccgtctgta gccattaccg ttcctgttcg
    37381 ttatctgcat tcccatacat cagtgattca tgaagatgat tatttgaata cagtgaaact
    37441 agtcacagaa ttagtgaaac ggcttgatag tgaaacagtt gaaaaaattc ggagttatga
    37501 ataatgaata atagaatacc tatttctgaa tatcggaaaa aagcacgagt ttctttggaa
    37561 aatcaatggg gaatcaatac ttggcttgtt ttcttatcta taatatttac gagcgtcatc
    37621 cagaactttt ttgacggttt cagtcctttt ccggaagaaa gcatgcagca gcacttattg
    37681 agttttctag tcgataattt acttttattc gcattcaact atgcattgtt ttacattgcg
    37741 ctagtagttg tacggggtgg cagaccaaaa agtaaccttt tattttcagt tttccaaaga
    37801 aagtattttg gttcgatact tggcatcaat ttgatcaatt cagtcatcaa ctggctactc
    37861 aacagtttaa tctttctgcc catgttttta atcagcggag tgagtactta tatgcagctg
    37921 cttttcagtt caaatgcggt acctacgggt gtcatgccag aagattttct tgatatcagc
    37981 tttgttttat cgatgatagg gatgattttt gtttcagcac tcatcatgag tattgttggc
    38041 ggtttattcc aatttgctgc atggacaaag ttcgactatc ctgatttgtc ggttattcaa
    38101 tgtttgaaat atggctggtt tttattgaaa gatagaattg gtacgtatat tttgttgcag
    38161 ctgtctttta tcggctggta tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg
    38221 gtcattgttt atgtgaatgt gtcagttgca gaattttatg aacaagcacg gattgaaaaa
    38281 ggaggtccag acgcctattt tacttagcat gtaagcagtt tattcatcaa aaaaataact
    38341 tttgtggtag gataaatctt tgagggggaa tgtacgatga aattaactgt tttaggatgc
    38401 ttaggagcgt atccttataa aaatcaaggg acaacaggtt atttgcttca atctgacggg
    38461 ttcaatttgt tgatcgatgc aggtagtgct acgctgatca aattacaaga acaccttgat
    38521 ccacttgatc tagatgctgt gatccttagc cattatcatc atgatcatat tgctgacctt
    38581 ggtgtattac agtattactg gcaacttcat cctaagagaa atccggagac attgctttca
    38641 atttatggac acacaaaaga ttccattcac tttgaagaat tgacgttgga aaatgtctca
    38701 aaaggacatc cttattttga agctgaagaa ttaaaaattg gtccttttct gattactttt
    38761 atggaaacaa tccatccagt agtatgttac gccatgcgta tcgtagaaga aacaacgggg
    38821 aaagtattcg tatttacagg tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa
    38881 gatgctgatt tgtttctagc agatacctat ctttttgaag gaaatgaaca tcatcatgcc
    38941 catttcactt caaaagaagc tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta
    39001 cttacacacc tacctcagtt tggtgattta gaacaattga aaaatgaagc gcaaaaggca
    39061 gcaggcaaag ggattgatat agaacttgct gcagttgaca aagtgtttaa catttaggag
    39121 gaagaacatg atttttgtac caaatgaaaa ccacgaccca agaatcaatt tagcaattga
    39181 acaatatttg ctgcaagaaa tgcctttaga tgaacctatt ttgctctttt atatcaatga
    39241 accttcaatc atcatcggca gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt
    39301 ggaagaaaat ggcattcatg ttgtccgtcg cttaagtgga ggcggagctg tctaccatga
    39361 ttttggaaac ctgaacttta gttttatcat gcctgatgat ggggattcat tccgtgactt
    39421 tgcgaaagta acacagccaa tcatccaagc acttcatgaa ttgggtgttg ctggggcgga
    39481 attgaaaggc cgaaatgatc tagtcattga tggcatgaaa ttttctggca acgcgatgta
    39541 tgctaccaac ggtcggatgt ttgctcatgg aacaattatg ttcgactctg atatcaatga
    39601 agtagtcaat gcattaaaag taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat
    39661 ccgttctcgt gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac
    39721 agaagaattt cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag tagaagaagt
    39781 aaaaacgtac gaattgactg atgaagattg gaaaaaaatc aatcgaatct ctgaggaata
    39841 ttatcgaaat tgggattgga attacgggaa gtctccagat ttcaatttct ctcgccaaaa
    39901 acgtttttct atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga
    39961 agcaaaaatc tttggcgatt tcttcggact aggagatatc tctgacgtgg aaaaaaaatt
    40021 ggttggacag aaatatgata aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa
    40081 gaaatatttt ggtgcgatag aaccagaaga attgcttacc ttactttatt gaaaataaaa
    40141 aatagcggaa tccacatata tggattccgc tattttttat acaggattcg ggtaatttcg
    40201 agagagttga acagtgatcc gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt
    40261 gtaacgatcc gctacgtctt tcatcatcat taagtcaaaa cgttcctgat catctaattc
    40321 ttctgtgatg cctcgactaa ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat
    40381 gacgaaatgt tcctcttgtt cgtgtatcga caaataaatt tttcgataca gtggattttc
    40441 gatggcatga gtttctctaa aggaatgctc taaagcgtgg atcaaaagtt gagccagatc
    40501 gtcatcgttg ataggaagtt cttcgggaag atctgcttgg aaggttatct gaatattccg
    40561 tttggcagct aacttctggt aacgcgctaa gataagattt gtcacctggt ggttgcaaaa
    40621 agaacacagc atttcgaaat tttttgcttc ttctaatatc ttttccacat agtttttagc
    40681 ttttgtttgg tccgtatgca ttaaatcttg cagattctcc atctcttgga caaattccag
    40741 gcgaagtgta tttacttctt ggatatgctg tcgaagctgc tcataatgtt gctccaacaa
    40801 atcatttttg atttttaaga aatctacttg tctccgatct ttgtcggtat ttaagatgtg
    40861 atcgattaga ttgtagctga taataacgat tagaatgata atatataaaa tcgtagacca
    40921 attgatcgtt gtttgataga tacctaatgc cgtttggatg taaatcaagc aatggataat
    40981 ggctaccaag acgatccaag gtgtacaagc tacataaaaa cggttattct tatatgcttc
    41041 gccaatgcat gcaagtgaag tgactaaagt gcttataaca ctagcaatat tagcaatcgg
    41101 cattaattca ggaagttctg caggagtaaa agttgcataa agcagaacgc cgataaaaat
    41161 caaaagttgg aacgtacaaa aaattccgta ccatttttta tagtaaaaca ttcggctcca
    41221 atagtagcag atgatgaaag ctgacgttaa gataataaat aaatgagata aaaaagaatg
    41281 aacgaatggt tcaaaaagaa taccacttaa aatatcttct gatacagctt caaatcctaa
    41341 tgccaaagca aagcaactta agagaataag tgagtattca agacgttttt gtttatacat
    41401 caagtagatc gtatagaaaa aggtccctat agcgataaaa acagcgatta tcatcgtcaa
    41461 tacttgcggc actgattgtg aataaataaa actaatcaat ggtccagttg ttcctagata
    41521 aactttcggt ggcagtccag catagttctg ataaggagat gagactgtaa gggtcaattt
    41581 ttttccgata taatttgctg gtaaatctat cacagccaat gagagtccgg gattgctttc
    41641 ttcctgataa tctttccgtt ggtataaaat cttttcgcct agatatacag taatccattg
    41701 atggtttcct ttgattagta aatttgcttc gtccaacggt ttttcaagtg tcgtttccat
    41761 aatgaatgtt tcgtctttat ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt
    41821 aaaatcagga tcatcttttg tatagaaaac ccacgaatct tttatttctt tcatttgatt
    41881 gctgtccgca ggagaaaaaa taaaataaaa gaaataagca attcctgtta aaaataacaa
    41941 tgataatata atgattatgc tccactttat acgtaaataa aa
//
LOCUS       DAZUMB010000013        35424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000013 DAZUMB010000000
VERSION     DAZUMB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.013"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..609
                     /locus_tag="ACG80L_000560"
     CDS             289..609
                     /locus_tag="ACG80L_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS5022844.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            763..1668
                     /locus_tag="ACG80L_000561"
     CDS             763..1668
                     /locus_tag="ACG80L_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022845.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1665..2438
                     /locus_tag="ACG80L_000562"
     CDS             1665..2438
                     /locus_tag="ACG80L_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5022846.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2619..4043)
                     /locus_tag="ACG80L_000563"
     CDS             complement(2619..4043)
                     /locus_tag="ACG80L_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5022847.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4254..4700
                     /gene="nrdI"
                     /locus_tag="ACG80L_000564"
     CDS             4254..4700
                     /gene="nrdI"
                     /locus_tag="ACG80L_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5022848.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4765..6747)
                     /locus_tag="ACG80L_000565"
     CDS             complement(4765..6747)
                     /locus_tag="ACG80L_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5022849.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6761..7657)
                     /gene="murQ"
                     /locus_tag="ACG80L_000566"
     CDS             complement(6761..7657)
                     /gene="murQ"
                     /locus_tag="ACG80L_000566"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5022850.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7660..8721)
                     /locus_tag="ACG80L_000567"
     CDS             complement(7660..8721)
                     /locus_tag="ACG80L_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5022851.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8932..9459)
                     /locus_tag="ACG80L_000568"
     CDS             complement(8932..9459)
                     /locus_tag="ACG80L_000568"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5022852.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9526..11028)
                     /gene="eat(A)"
                     /locus_tag="ACG80L_000569"
     CDS             complement(9526..11028)
                     /gene="eat(A)"
                     /locus_tag="ACG80L_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5022853.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11286..12407)
                     /locus_tag="ACG80L_000570"
     CDS             complement(11286..12407)
                     /locus_tag="ACG80L_000570"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5022854.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12533..13474
                     /locus_tag="ACG80L_000571"
     CDS             12533..13474
                     /locus_tag="ACG80L_000571"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5022855.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13624..14520)
                     /locus_tag="ACG80L_000572"
     CDS             complement(13624..14520)
                     /locus_tag="ACG80L_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5022856.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14789..16129
                     /locus_tag="ACG80L_000573"
     CDS             14789..16129
                     /locus_tag="ACG80L_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5022857.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16347..16790
                     /locus_tag="ACG80L_000574"
     CDS             16347..16790
                     /locus_tag="ACG80L_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS5022858.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17294..18712)
                     /gene="nhaC"
                     /locus_tag="ACG80L_000575"
     CDS             complement(17294..18712)
                     /gene="nhaC"
                     /locus_tag="ACG80L_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5022859.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19115..20338)
                     /locus_tag="ACG80L_000576"
     CDS             complement(19115..20338)
                     /locus_tag="ACG80L_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5022860.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20369..21208)
                     /locus_tag="ACG80L_000577"
     CDS             complement(20369..21208)
                     /locus_tag="ACG80L_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5022861.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21225..21905)
                     /locus_tag="ACG80L_000578"
     CDS             complement(21225..21905)
                     /locus_tag="ACG80L_000578"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5022862.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21907..22980)
                     /locus_tag="ACG80L_000579"
     CDS             complement(21907..22980)
                     /locus_tag="ACG80L_000579"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5022863.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23036..23274)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23317..23550)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23627..24298)
                     /locus_tag="ACG80L_000580"
     CDS             complement(23627..24298)
                     /locus_tag="ACG80L_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5022864.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24385..27033)
                     /gene="mgtA"
                     /locus_tag="ACG80L_000581"
     CDS             complement(24385..27033)
                     /gene="mgtA"
                     /locus_tag="ACG80L_000581"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS5022865.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27375..27549)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27722..28159
                     /gene="ndk"
                     /locus_tag="ACG80L_000582"
     CDS             27722..28159
                     /gene="ndk"
                     /locus_tag="ACG80L_000582"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS5022866.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28181..29047)
                     /locus_tag="ACG80L_000583"
     CDS             complement(28181..29047)
                     /locus_tag="ACG80L_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5022867.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29207..31300)
                     /locus_tag="ACG80L_000584"
     CDS             complement(29207..31300)
                     /locus_tag="ACG80L_000584"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5022868.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32049..32672)
                     /locus_tag="ACG80L_000585"
     CDS             complement(32049..32672)
                     /locus_tag="ACG80L_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022869.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32831..34228)
                     /locus_tag="ACG80L_000586"
     CDS             complement(32831..34228)
                     /locus_tag="ACG80L_000586"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5022870.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34260..35231)
                     /locus_tag="ACG80L_000587"
     CDS             complement(34260..35231)
                     /locus_tag="ACG80L_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5022871.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
BASE COUNT        10180 a         7429 c         6215 g        11600 t
ORIGIN      
        1 ataaggtcta gttactgttt tttttaaatg attaataaat aacaacttct ttctatgaaa
       61 ttgcaaagca ttcatcttct tttccgctaa cgatatatcc gtcagtgctt ggtacacttt
      121 actaaaaagt atgagattgc cgatatctgg ataggtgtgc tatactacag aaaaagatat
      181 acaatctgcc accgggtagg ataaagagga tgatcattcc attaggtctt tgaagggatg
      241 attttcctct ttttgtgtat ttttttatag ttaaggagga atgtaagtat ggcatggatg
      301 gaattaatca tagcaggaat tttggaagta ttctggtcaa cgatgatgaa atggagtgat
      361 ggtttttcta aaatcaatta ttctttttac acagtgattg gcatgattgc tagtttttac
      421 tttttgtcaa aggcaatcaa aagtttgccg atgagtctct cttatcctat ctggacaggg
      481 attggcgcag ttggttcggt cataattggt gtggtcttgt ttcatgataa gctgaatctt
      541 tccacttggt tctttgtggg gatgctactc atcagtatca ttggcatcaa gattactagc
      601 ggccactaga cacttggaat aagtactgat cagaaaaaat aagccttttt atttaatatt
      661 caattttttt atgaaaaaat aagaaataag tccaatgacc tagcgacttc tccactcatc
      721 aggtgtatgc ttgtactatc tatagaaaag gagtgaatat ttttgaaaag tctttcggtc
      781 caagaattat ccaagaactt tggtcacaaa caagccctca gcgatgtcag ttttagctta
      841 tcccctggca aaatcgttgg attagtaggt cctaatggcg ctggaaaaac aacgatcatg
      901 aaagctattt taggactgat ccattactca gatggaacaa tctgtattgg tgaaaatgag
      961 atttctccct ctagtcataa agggttggaa caagtaggag cattgatcga atacccgggt
     1021 atctatccat tcttaacagg ctatgatcat ctgaagttat tctctgaaac caacgatgta
     1081 tctgctatcg acactatcgt gaatcagctc aagatggaaa agtacattca caaaaaagcg
     1141 aaatcctact cgttgggaat gaaacaaaaa ttgggaattg ctcttgcctt actgaaccat
     1201 ccgtctatcg ttattttaga tgagccgatg aacggtttgg atccacaagc aacaagagat
     1261 gtgcgaaaaa tcattaccga cttggctact cagggcacct ctttcttgat ctccagtcac
     1321 ttactcagcg agcttgaaaa aatggtcgat gagctgatcc tgatcaatca aggaaagatc
     1381 atcaaacaat gtacaatggc tgaattgaac caaacagcgc aagatttcat cgttatctct
     1441 acaacaaatg atctagctgc tcgaacgatc ttgacagaag ctggatatac tttggaagat
     1501 cctgataaaa tcaagctttt aaaagaaaac gaagaattat taggaaccgt gatccagcta
     1561 ttgtctgcac aaaatatcca tgtgacagac gtccagcatc tgacgaatga tttggaatct
     1621 tctctattgg aaattctgga ttcagtaaag ggggaagaag aaaaatgaca ttgcttgtaa
     1681 aacaagaatt atttaaattg atcaagaaaa agtcaactgc tgttttatct gttctattag
     1741 ttgttttgct cataggaaca gcacttctag ccaaaaaata cacgacgatc attgatccag
     1801 ttgaaatgac tgctcagctt tttagtgcca catcttggat tgtctttatc atgattgcag
     1861 ctgcaagcac gatcatttcg atggaagcac aatatggtac attgaaaaat ctcctttatc
     1921 gaaagtattc tcgaggagaa atattagtaa gcaaatggat cacattagtt atttattctg
     1981 tttatttata ccttttagct attattgtca ctgtgttgat gaaactgatt cttttccctt
     2041 cgatttcctt cacggagaag gtttctactg gtcaaacact gatccaaagc ttgttcactt
     2101 atactttggg tagttatatc ggtttatggc tgattttaag tttagtgttg atgattgctt
     2161 gctttatcaa tagttccggc gcatccatct cagcaggaat cgtcttttat tttgcttcgt
     2221 caatcatatc tggtatcctt attgcactca tccaaaaatg ggagtggatc aaatggaatc
     2281 cgatcagtat gttgaatctg caaaaccaaa ttggaaacga agagatcatg aagcaattga
     2341 ctcatctttc tactaatcaa atgctattcg gtaatttagc ttacatcgtt ttgttcttag
     2401 cattaggata tctcgtattt aagcgaaaaa atgtatgatg actcatcaaa aaagaggctg
     2461 tgaaagagac gttcagcctt attgccgatt ggctgtctct taaacaccgt ttattctgaa
     2521 aaaggagctg tgacaacatt tgtgtcacag ctcctttcct agtatgtttt tgaataaacg
     2581 acaggctcac aacctctctt tatacttctt ttatacttct atacgtgaag catcccagtg
     2641 gcaaataaaa ccattgcaat cactccgacc atcgtcacac ctataagtaa aatcttatct
     2701 aagtggatat gttgtacagg ctcgttttgc tccttacgtg ctttgaggta aaaataaatg
     2761 cctggcaaga aaagaacaag aacaagcatt acttcattaa aggctacgac tagcatagcc
     2821 catacttgaa aggcacttgc taaaagccct actaaaaatt ggacccattc tttccgctga
     2881 taagaaagtt tcatttggta caatcctaag aaaagccacg tgaagaaaat agctgctgta
     2941 ccaagtgtgt aagcaaagtt ataagcactg tcagtgaata ataatgaaag taaaaagaca
     3001 ttcgtacata acgtcgtcaa tacgatagca taagttggtg ccccattttt gttgagtttt
     3061 ccccatgctt taggcaaaag tccgtcttca gccatgtttc ccattgtttc aatcggtaag
     3121 atcgtccatg agagccaaac accaactaaa gaaatgatca aaccgccgtt gatcacgact
     3181 gctccccatt taccaaccat catttcaaac acatagccca tagcaggctg cgccttgatc
     3241 gtttgcagct gattcatcgt cattgtccca tatggaagca tggaaaccaa gacgtaaata
     3301 ataatcaagc cgaatatccc tagaatcgtt gctttcccaa catctgattg tttttttgct
     3361 cgtgaaccta cgacagaggc tccttcaata ccaacaaata cccaaagcat caccatgatg
     3421 caactcaata cttgactgga aaacgggatc ggctgtccat tcgcattcaa tataatgtcg
     3481 ctagaaaagt tatcaatgaa ttgttggatc gaaaacgtgc tgatagctac tacaataaat
     3541 aaaaataatg gcactaattt acagaatgtg acgatagcat tgacgaaact agcattttcg
     3601 actccccgat tcactagcca tgcatatccc cataaaaaga ctgtactcat cacgatcgat
     3661 gccagatttt gcccaccttc aaatactggg aagaaatatc ctaatgcgct catcaacaat
     3721 gttgcaaaag ctacgttgcc taaccagcat gtgagccagt aaccccaacc gctgataaaa
     3781 ccagcgaaac ggccaaaacc ttcttctgcg taactaaaga tcccgccttt taaatctgga
     3841 cgttttttat tgatattatt gatcgacaag gagagcatca acattgctgt tcctacgagt
     3901 acccaactga aaatagctgg gccgccagcc gatccggaga tgtccgtcat aataccaaat
     3961 acgccacctc caatcgctga accaattact aaggcagcaa gtggaattaa accaattcct
     4021 tttttctgtt gctctgtatc catgtaagca tcctccaaaa taaaaccgct tttatcaaaa
     4081 agtataacaa acctttttta tttttactta attaataaaa actttaatag aaatataatt
     4141 ctaccggctt ttttctcatt atacaagcaa agcgcctagt ccctttccaa attacaccac
     4201 caaaaaactt aaaaaagatg ctatactaaa aggaaagaat tggaggatga tccatgaaca
     4261 ttttatatat atcgatttca ggaaatacgc gtgcctttgc caaacatcta gcagaatatg
     4321 ctgaaaaaat gcatgcagaa gatcctgtca atcctgaagt tactttaaaa gaaatccatg
     4381 aaaatagtga ttttacgaaa gaaacagaac cattttttac tttcgttcct acttatttag
     4441 acggcggaaa tggtttagat aacggtgata cagaaatctt gaccgaaacg atgcgtgaat
     4501 atctggaaca tgaagacaat cataaacttt gtcttggtgt agtcggtagc ggaaataaaa
     4561 acttcaataa tcagtattgt ctaacagcca aacaatatgc acaaacattt ggctttcctt
     4621 tcttggcaga ttatgaatta cggggaacac catctgatgt gacacgaatt tatcagattt
     4681 tagcagaaaa caataaataa taaaaaagaa gaagctgaaa aatagatttc agtttcttct
     4741 ttcgtttcta ttgtttctat catattaagc tttgacatcc atcacaggtg cttttgcttt
     4801 tgctttacct gttttttcca agacaaagtt ttttacacga tccatattgg ttaccaagac
     4861 gatcatggat gtttctttgc ctgctgcttt gatcgcttct aagtcagctt ctgctacaag
     4921 cgtatcaggt gtcacttgct gcccttctgt tactttcaca tcaaatggcg taccgcctaa
     4981 gtcaactgtg ttgattccca tatgaagcaa gatttctaaa ccatttggcg ttgtgatccc
     5041 aatagcgtgt ttcgttggga agacagagct gactacccca gtaactggag aatagatatg
     5101 tccgttttct ggttctacaa agtagccttc acccatcatc ttttgtgcaa aaactggatc
     5161 acttgcttta tctaaagaaa caactgttcc gtcagcaact gatgagagga tagtcacact
     5221 ttcagctgaa gtttgaggta ctactttttc aggcacttca tttactgtgg ctgtttcagt
     5281 cgctaattct tctttttcta aacggtcttt aggaataccg aagaagaatg tagcaacgaa
     5341 gccaccagca taagctgcca ataatccaag gatgtagcct aaccatttac cgtcagagat
     5401 caatgggatc aaagcggctc ctgaaggtcc aatagcgata gccccgacat ttccgatcat
     5461 tccgatcact gccccgccga tacctccacc gatacaagca gtaataaatg ggcgtcctag
     5521 aggtaatgtt actccgtaaa tcaatggttc gccgattcct aggatcccga ctggtaaagc
     5581 tcctttgatc atttctgtta attctttatc ttttttgcag cgaatccata atgccaatgc
     5641 tgcacccact tgtcctgcac cagccatcgc aagaattgga agcaatagcg tacgaccagt
     5701 ttcatcaatc atttgcacat ggattggagt caaaatttga tgcagtccga acataaccat
     5761 cggcaagaat aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc caagtaccca
     5821 gttgatccca ccgaccatgc catcagaaat gaaacctgct aaaggcatga tgaagaaaat
     5881 ttcaatcaga ccgatggcaa tcaatgaaag agttggtgta acgatgatat ctaccgcatc
     5941 aggtactact ttatgcagtt ttttctcaac gattgacaat agccaaacag cgaagatgac
     6001 acctaggatc ccaccttgtc cagctgcaag agcttcacca gtaaataagt ttttcagtgg
     6061 tgcttccgga ttcatccctg tcagcatcac aacggccccg ataactccac caagagttgg
     6121 tgttgcacca aaaacttgtg cagcattgat acctgtatag ataactaggt agctaaacat
     6181 cccgtttttc aagatgttca tcacatcgat atattgttgc catgttgctg catcaagtgt
     6241 tccggctgtg actaagtttg ctaaaactgc ggcaatccct gccacgatcc cagtaccgac
     6301 aaatgcagga atcattggca caaagatatt agaaatgtct tttaataccg atttgatttt
     6361 agatggtttt tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt cgtttacacg
     6421 gtcttttcca gatgctgatg aatttgctgt agctgtactt acagaagaaa gtttttctcc
     6481 taatttcacg cctgccaaat caaccatttg ctgtgctact ttgttcactt tcccaggtcc
     6541 gatgatgact tgaagctgtt catcttctac tacaccaagc actcctggaa tcgcttttaa
     6601 ttcagagaca tttacaagat catcattgat gatcttcata cgcacacgag tcatgcagtg
     6661 gacaatgctt tctacattgc ccatgccgcc tgccccttta taaatttgtt ttgccagttc
     6721 ttccaaagtc agttcgtttt ttgccatggt cttttccccc tcataaggtc ccttttacaa
     6781 atccgtttgc ttcagctaat tttgcttgag cttcctcttt atcactattt gtcaaaatca
     6841 tgacgatagc cagcttaaca ttttgatctg cttcttcaaa gtacttttca gctgtttcgt
     6901 aatcagcttc tgtcgcttgc atgatgattc gttttgagcg ttcgattagt ttttcatttg
     6961 ttggtttgac atcgaccatc aagttattga atactttacc tataccaatc atactgatag
     7021 tagaaatcat attcaagata agtttttgtg ctgttcctga cttcaatcga gtagagcctg
     7081 tcaaaaattc tggacctgca tcgacttcga ttgggaaatc cgcgtgttta ctgatttcgg
     7141 cttgtttatt gcaagaaatc gttcctgtga cagcaccgat cgattttgcg taatctaatc
     7201 caccgatcac atatggtgta cgaccgctag ctgcgatacc aataactata tcattttttg
     7261 tcaaattcag cttgattaga tcttcttgcc ctagctcata agaatcttcg gccccttcga
     7321 cagctacagt cattgcttct tctccaccag caattagacc tatgaccatt tccggttcta
     7381 caccgaatgt cggtacgcat tcagcagcat ctaacacacc tagtcttcca cttgttccgg
     7441 ctcccatata aatcagacgt ccaccttttt taaaggactc aatcgtgtta gcaatcactg
     7501 gttcgatact tgtcagttct ttttcaaccg cagcggccac tttctgatct tctttgttca
     7561 ttagttgaac ggcttgagaa atactcatct catctaatcc aaaagtttct tggtttctgc
     7621 gttccgttgt taggttctct aaattgattt tgctcatttt tatcttcctt cttttcttat
     7681 aaattgatag ttcataccag ctttgatttg gttgatcaat ggtagctctt ctttgataac
     7741 tttagctact acattcactt tttcatccgc tggcaaatca tatttggtta attggatctc
     7801 acccatatag cgcaaatact tttcgttatc taaagtaata gaccctttca tacgcgtagc
     7861 tgtatttctt gctgggatag gcggaatctc tcgaaaacgt gcatctacgc ttcgtatgac
     7921 atctcttgca tcgtcttgcc gattcgtatg cttccctaat atatattcgt aaaattcttc
     7981 atctacaggt tccgtatgga gcaagactgt ctgttctttt tggaaagaag caaattgctc
     8041 ctgaacgttt tctgacagac ctgcatctcc gatgtaaaca atatccgtgt tacttgctaa
     8101 tagatctaga gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag ttggcaatcc
     8161 ttgatatagt gggcctctca actctgtatc tcctggtaca aaaccttgga caaggagacc
     8221 ttgaattttg agccattgat tttttttctg ataccattct ttgtccagcc ctgtttcggg
     8281 acgtggataa taattatgcc aagcttcaat ctgtgagaaa tcagcttctg cttctttcaa
     8341 ttcatcgata tctttaggcg tgatcgtgct agcattcaaa ctgattttca attgatgtga
     8401 ccagtcggca atctgctgat tggaaatatg ataatccatt cttaaacctg ttacaccaat
     8461 tgctttcaaa ggcgttagat cttctaatga aaaacccgct cgttccaatg cctctcctga
     8521 aatatcgacc atcaattcta gtttattctt tttcgcttcg cttcctagtt cgatcaatcg
     8581 cttttgatat gccgaaacat catcttctgg tatatgcatg gaagtgaaaa taccgacaaa
     8641 gccattggat gccatttggc ggatatactg tttcttctct tctgacaaag aatcgttcat
     8701 aaatattgaa aagccaaaca tattatccct ctttttctgt atccgtcttc atttctatga
     8761 ctagagtata tatctaaaga aacaaaattt caatactttt attaaaataa ttaaatttca
     8821 tttctaaagg ttgagtttat attatcaaaa gaattgtcat aacctaaaaa aggcactgct
     8881 aagcaaaagc agtgccttga aatattttat ttaaataaca caaaaacata attactcata
     8941 agaaaccaat tctgtattat ctaccatgaa tgtaaagaac ccctcattag gcatcggatg
     9001 aaaataagca ttgatcactc ttatcaaggc attatggcaa actaacagat agacattttc
     9061 ttcgtcttcg atgcattctt tcaataaagg aacgatccgt gcatacacat ctaagactga
     9121 ctccccattt gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt
     9181 gcttggcagt ccatcataat ccccaaaatc catttctacc aatcgttcat caacggtcag
     9241 cggcaagctg agacgatcag caactgcttg tgctgtatct cttgctcgtt gaagagggga
     9301 atggatgatt ttcgtgattg gaacaggcaa atctactacc ttttctgcta gtttctgtgc
     9361 ctgcatctgt cctttttcag tcagcggcac atctgcccgt ccgcaaacta atccttgtgc
     9421 gttccaagtt gtttctccgt gtctcgttac ataaagcatg tgattctctc cttcgttggc
     9481 aaagttcccc ttaaccatac cttgttgttc ttgagattac aagggttaga ttctctcaag
     9541 agaaataatc tcagtagata ctttatccag gaaggtttga tcatgttcca ctagtaacat
     9601 ggcaggtttc acgttcaaga tcagttgttc taattgttct tgattgaaga catccaaata
     9661 attcaatggt tcatcccatg tatatagttc agctggctgt gataaagatt tagccaattc
     9721 cacttttttc cgttggccca tactcatctg ctcgatcttg ttatgaaaaa catctctttc
     9781 catcccaagc tttcggaggt tgttcaaaaa tgcttggtag tctacttggt ttttctctgc
     9841 aaattccgcc aacgttcctc gattgtcttc ataattttgg cttgcataac taatgctcag
     9901 atgttttggc tggtattttt ctcctataac cttgccgttg aatgccccca gaagataatg
     9961 gataatggat gactttcctg caccgttagg acctgaaatc gccacccgct gatgaggctc
    10021 gattgtaaaa tgaattggct cgaataacag attttcatac cctaattgaa gatcttctac
    10081 gcttaaaagt cgcttatggt gagacgcttg gctattcatc gtcaaagaat cgatatattc
    10141 gatatctttt aatagttttt ctttctcgct gatctgggtc tccatccgat tcacgattgc
    10201 tttggatcgt ttcatcgtcc gtgcagcatc agcacccact gctcctttat tcactcgtct
    10261 agattcagta tcgatgaatc cgacttgttt ctttgtttta tctccttctc gggaacgaga
    10321 ccattcggct ttttcagctg ctgttttctt tagcctactg acttcttttt tcaatttttc
    10381 attttgagcc atttcaaatt catcacgaag ttttttctgt tcttcataga tagagaaatt
    10441 cccttgataa agttccagtt gacttttttc gattgctaaa acatggtcca ctacttcatc
    10501 gataaatccc cggtcatggc tgaccacgat gaagccttgt tttttctttt tcaaataagc
    10561 cgctacttgt tttctaccag agatatccaa atgattcgtt ggttcatcga ttaacgggaa
    10621 atgagtgtca tccacaaata aaagtgccag taggactttt gtcttctctc cccccgatag
    10681 tgtgctgaat tctctccata agatttcagg atctgtttgc atcaattgga gctctctttc
    10741 gatttcccat atctcaaaat ccgtaatatc atttaacacg taataggtta aacgttcttt
    10801 atcttttgtc tgttgcggga aataggcaaa ttcttgctga tggattactt gcccttgata
    10861 aggtagtttg ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat
    10921 aagtcctagt ttccattgtg tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc
    10981 ttggctgtcg tagccgaatg tcagattttt tatttcgatt ttagacattt tccatcacct
    11041 cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggatac
    11101 acacaaaaag acccatacat cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt
    11161 ccttgtctcc acaaggtaca gtctgaatgc agctttcatc aatcgactta atttttcaat
    11221 gcacggatcc ctcactttgt cctatatttg aaattacgat agcataaaga aaatgatttt
    11281 gtcaactact tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt
    11341 ttttctaatg tttgcctgtg ttccattgct tcttctaaac ctattggacc caaatgaata
    11401 cttaagacaa ttgggagaaa atcagaaagt ccttctagct cttggtctct acttccgcaa
    11461 atagcgatta ttggttttcg atattttttt gcaagcactg ctacaccgta tggaactttt
    11521 ccgtggatcg tttgctgatc gatccgccct tctcctgtaa tgatgacatc tgcttgttcg
    11581 atcttttttt ccaatccagt gatttggctg actaaatcga aaccagaaac catcttcgct
    11641 cccaacaaaa aaagggctcc accaataccg cctgctgctc cacttcctgg aatatcatct
    11701 aaatccacct cccagaaatc ttttatttgc tgagcaatct ttcctgcttg tttatctaag
    11761 aaagctagcg tcgtatctat tcctcctttt tgtcttccaa attgccaagc agctccttgt
    11821 tgacctgtat atggattggt aacatctgca gcgataatca attttacttt agaaacaaga
    11881 acgtttcttc gatcaaaatg aagtttttta gtagataata aaggatttcc ttctgtgtac
    11941 ccttctaaag aagcacccaa ggattggagt aatcctagac caccatcgct acacccgctt
    12001 cctcctaaag tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct
    12061 cctaaggcat aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct
    12121 aaccccaatg cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat
    12181 aaatagctga tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt
    12241 cccctgagcg cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca
    12301 ttgatttttc ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat
    12361 gaagaagcac ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac
    12421 tttcttctta agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt
    12481 ccatttttct atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat
    12541 cgcaatagct ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg
    12601 aaatgaggtc ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg
    12661 gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga
    12721 agaaatcatc gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca
    12781 attagatgat atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg
    12841 tttactaaat ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat
    12901 cttatttggg atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt
    12961 aggtgatgga gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa
    13021 agttgtagaa gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta
    13081 ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga
    13141 ttgtaatatt gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt
    13201 atctgaattt gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta
    13261 ccaacatatt gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta
    13321 ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg
    13381 gcgaaaagga caaaaatatg atattgctac accatattgt gcctttttga cccaattagt
    13441 ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga
    13501 atcagcagct ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct
    13561 catgatgcca taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt
    13621 tttttatttg ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc
    13681 gttagtgata aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag
    13741 gattggctta aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag
    13801 caaaaaaata ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac
    13861 tagtttcttt tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc
    13921 ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt
    13981 aagatatcca ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc
    14041 tctttttata aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc
    14101 accaaaaaat agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa
    14161 tgaactatta tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc
    14221 atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg
    14281 attccaaaaa aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc
    14341 catatcaaat ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc
    14401 tgatttaatg aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat
    14461 ggaaggcaaa aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat
    14521 cttttccgcc tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa
    14581 gccagtaaaa cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga
    14641 aataaatcat tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg
    14701 aaaatttgtt atcattagtc attgttaaat attgctattt attcttaata taaattttga
    14761 ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag
    14821 ctccagacat atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg
    14881 aagtggcgaa gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg
    14941 gctgatttta tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac
    15001 gcatcacgga ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac
    15061 tgattgggtc tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc
    15121 ttcctttttg cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc
    15181 tgttttaact acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg
    15241 gctggctttt gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt
    15301 cggtcagcaa atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg
    15361 tgggtttact cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc
    15421 ttacggcggt tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc
    15481 gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt
    15541 gtttttgctt ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt
    15601 tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat
    15661 tgtccttgct ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt
    15721 cttccgtttg aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca
    15781 agtaccgcaa cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc
    15841 ttattttgtc ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt
    15901 attgatctgg atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt
    15961 atgggataaa ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat
    16021 cctgttcacg aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta
    16081 tttcagttat caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa
    16141 aattttattt tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag
    16201 tcttgttgca actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc
    16261 aaaaaccctc tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag
    16321 gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg
    16381 gtgtttcaac ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata
    16441 tcttcttcag tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca
    16501 tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag
    16561 ccgctagtct tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca
    16621 acgacacagc ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta
    16681 aacgagctga tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac
    16741 aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg
    16801 tccaaataaa aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta
    16861 cttctggaac gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa
    16921 cagccaaaca aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc
    16981 aagcttttga acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag
    17041 agagacaaat ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt
    17101 cagctgattg aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta
    17161 agaaaaattt tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa
    17221 actggctttt gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc
    17281 cctttttatg tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca
    17341 caaatttttt tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa
    17401 gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta
    17461 cgaaacttcc ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta
    17521 gcgttctacc taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat
    17581 taccaggcag aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg
    17641 tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca
    17701 tcacggttga gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa
    17761 tcaatgacac agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt
    17821 gttggtttcc agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg
    17881 aagtttgtgg gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg
    17941 ttaaaattgc cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg
    18001 accagattga aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa
    18061 gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg
    18121 ccgggacagt tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag
    18181 ctgctgctag atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg
    18241 aaacaatagc tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa
    18301 atgcaatccc aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg
    18361 cacacacaac aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga
    18421 ccattagagt tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa
    18481 taaaaataaa gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc
    18541 aatctgtcgg gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga
    18601 tcgtgatatt aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa
    18661 ccattaatac aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct
    18721 ctcctttttt agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca
    18781 attcatccgc gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat
    18841 gactccgcgg taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc
    18901 aaatgtatgg actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc
    18961 gactttctga acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta
    19021 ccttccataa gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta
    19081 tttttataac tttcacacac aagtttattt tttactagaa agtagcgccc tcttgttttt
    19141 ttcttgctac tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca
    19201 acagatcaag agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt
    19261 tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga
    19321 ttgctaccgt aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac
    19381 cgagacttgt tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat
    19441 gctggaagaa taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga
    19501 acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca
    19561 ctttttcata tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag
    19621 cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat
    19681 tgacatcttt tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg
    19741 cttccaaagc atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa
    19801 atttgatgtc gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg
    19861 gatggacgca taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt
    19921 tagctgatcc gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac
    19981 cagtttcatc aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca
    20041 aattatgccc gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa
    20101 ttgtgggacc gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta
    20161 cttctttttc tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat
    20221 aaaactcata gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt
    20281 ctggtaaacg atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa
    20341 aaaattcctc ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca
    20401 cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt
    20461 ctactatttt ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca
    20521 cataaggacg tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa
    20581 atccagcatc tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac
    20641 gtgctgtttg ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat
    20701 cagaaacagt gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca
    20761 ataacagaag agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt
    20821 ctttgatatc agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag
    20881 tatttccgat cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt
    20941 gatgttggaa agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt
    21001 aatctgtaaa tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt
    21061 tgtcccatac atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt
    21121 cagttgtatc tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa
    21181 tgactacaaa acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt
    21241 ttaatcagtt gattactgat aaattgactt aaaaaaacta agattagaat caataatgta
    21301 gccacaatcg ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct
    21361 aatccgcctg cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc
    21421 aacgccgaga cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata
    21481 ccgattggac tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc
    21541 agtgcgttct cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa
    21601 gctgctttcg caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa
    21661 agcaagataa tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat
    21721 agcagacggt tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg
    21781 attccaatca tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc
    21841 gcttcgacaa attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca
    21901 gtcattttat cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa
    21961 aatatttatt tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt
    22021 ttctccagct aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt
    22081 gccatataaa atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc
    22141 gttcgtctga tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc
    22201 aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat
    22261 aaaatctttg gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc
    22321 aacgattctt ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat
    22381 ctcatgagtg atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc
    22441 aaggatctga agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac
    22501 cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag
    22561 ttgtttagga taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac
    22621 tttttgacga cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc
    22681 aaaaatagtt cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct
    22741 ttcttttcgc aattctttgg gagagagctg actcagattc gtctgattga caatgacttc
    22801 tcctgctgtt ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc
    22861 tgagtaaccg acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac
    22921 tgcctgtatc tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat
    22981 taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga
    23041 ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt
    23101 caagtacgat catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc
    23161 tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac
    23221 cgaagctctc tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca
    23281 aaaaaaaagt ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac
    23341 cacctttttt cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt
    23401 tcgttttaac ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg
    23461 ccattttcag cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc
    23521 caaacttact ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag
    23581 gaaaactgaa aagtcaagta cgaaattcaa attatttttt ctatatttat ttttctgttt
    23641 ttttatttaa catttcaata acagttcgaa gatcttttcc ttccgcaaga agtttcaatt
    23701 tttccttttc ttcgacggta taatttcgga caaaactgga atcaggtatc aaaacaacgc
    23761 ctaatcctgc tcgtgaagca gctgttgctc ctactaaaga atcttccact gcaaaggctg
    23821 aactagcttc taatcctgtt cgttccaaag ctgtcagata aggaatcggt gaaggcttgt
    23881 tctccgacac ttcgtctccg aatgtaaacg tgtccaagta atcagctaat ttaaagtgat
    23941 ccaagatatc tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac
    24001 gttcttttaa gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg
    24061 tcagctgttg ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc
    24121 tacctgctgc tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt
    24181 caacgatttc ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac
    24241 gatacgtttt ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct
    24301 atacattaat cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaagaaca
    24361 gaagcaaaga accagccttc tcgtttataa gagttcttgg aatcgacgga tatagatttt
    24421 cttcacagct gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta
    24481 attgccagga agtggcatca agcctaattc agcaccaaaa cttgtaaaag gaaggacaga
    24541 accgacaata attcctaatg tagtgataac aaacatggca aatgttgcgt tgctttggat
    24601 aaatggtact ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc
    24661 gacaaaccat cctgcatgga ataatgcgat gaaaatcact cgctgtgaac tatccaatgt
    24721 atggaaactt ccgcccacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt
    24781 aatatcaaat atcgaacttg ttggaccaaa gtaaaccatg aattccccaa tactagatgc
    24841 attccatttt tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat
    24901 gcaggaaaca tcatagatca gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa
    24961 tggcaggaac gtacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt
    25021 agccttgata tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc
    25081 caaaatcgtc aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac
    25141 agctgtatct acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc
    25201 atctcctaaa aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt
    25261 ttgctgagga ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg
    25321 atcactcatt tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg
    25381 acggcacact gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc
    25441 atgttctttc agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagc
    25501 aagataacca atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct
    25561 tggattggtc ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac
    25621 ttttgcaagt acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc
    25681 aataaattta gaaatgttca gcatttcttc aatcgcgcct tttgtgatca tttgtgtttt
    25741 accagctgga tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc
    25801 atccactttt tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat
    25861 agccacatct aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg
    25921 caaaacacga tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc
    25981 ttgtgtcaat gtccccgttt tatccgtaca taacacatcg attgcaccaa agttctggat
    26041 ggcattcaaa tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa
    26101 attcgtcgta acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc
    26161 aaaaagaaaa gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg
    26221 agccatcaat gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa
    26281 gcttgattga attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt
    26341 tccaacagaa acaatgattc cttccgcact tccactgatg acattacttc ccataaagac
    26401 aagattttcg taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc
    26461 tactggatag ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa
    26521 taaacgcata tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac
    26581 gatttcttcc ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt
    26641 cacaagtgat ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat
    26701 tgtcccgctg acgataacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc
    26761 aggagctgct aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa
    26821 tgtaaatggc gtgatgtatg ctttcaatat ttctacaatg aatggtgttt ttttgccata
    26881 tgaaatcgca ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc
    26941 atttgctgat gcatggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc
    27001 atagattttt tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga
    27061 cagctgttct cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga
    27121 aaggggctga gaacagatag ataatagccc tggcctttcc tctagatcct tgtttttcga
    27181 atgactgtca tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca
    27241 atcatttttc gaatagttat ttttccaatt agaccacctc ctttttattt ttcaccgatg
    27301 gggatttgcg gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca
    27361 tattaaaaaa agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc
    27421 agatacctgc ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca
    27481 ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca
    27541 aatcttattc ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt
    27601 ttccatgttt ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt
    27661 tatttgtctc attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt
    27721 tatggaaaga acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag
    27781 catcatccaa cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat
    27841 gactcctgaa ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga
    27901 actgatcgat tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt
    27961 gatcgatatc gtccgtaaga tggttggagc aacaaaagct gccgatgctg ttccaggtac
    28021 gatccgagga gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg
    28081 tgatgctgcg gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc
    28141 tgaaaaagcg gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa
    28201 tggccagcag ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca
    28261 ctgtgaagaa ttcttggtag tctttcgtct tttcttcccc taaagcagca gaaatcaatc
    28321 tttgataata atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgcta
    28381 agctccgttt catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat
    28441 aaaacgaagt caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg
    28501 ctaatcgtcg cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga
    28561 atatccaagg aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca
    28621 tcaagtattc ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata
    28681 cttctgtttt ttcgaccatg tgaagattca aaaccaaata atacagatct tctgttttct
    28741 taaaatgcct acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc
    28801 gatctagcat tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc
    28861 attcatattc tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg
    28921 acattttttt cactgaattt gtctcaatcc cgactagaga agtcttaaaa cagattcctt
    28981 ccgctaattg ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat
    29041 catacataat agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt
    29101 aaaaaggaaa aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct
    29161 tcaagcatta gaatgttgtc taactactga aggttggttt gattgtttat tgattatttt
    29221 tttgctgttc aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattaccat
    29281 tttttgaaaa gacacttgtc gaatgttcgc ctttttgctt ttcgattgct tcattacgtt
    29341 ccgcttcatt tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat
    29401 aaaaccgcaa gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt
    29461 tttcctgatc tttggagatt tcctcatcga ttcctgctgc tttcgtatcg atgatttctc
    29521 ctgtctgatt attgtaagct tttcctccta ggacggtgta tttgggtgtc acaaagttcc
    29581 catttcgaaa agcaactact tgatcatgct gagaagataa taagtctgta ccaaaatgaa
    29641 gataatcttt atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc
    29701 caccgtattc atgattgact gtccctttag tataaccagg catatggatc atcaatggca
    29761 ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcgc
    29821 ttgctaaatc tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga
    29881 ttgaattttg gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat
    29941 ccagataatg tgccgttcga acataattat ctactgtgct attgccagta ttcaaagatg
    30001 gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt
    30061 tcgtgtaaaa cggctgttgt agatgctcaa gatattgggc agattctctg aataaatctt
    30121 tatctaatat cccataacct aacgtttcgt cggattcatc aaaataagat cgatcaaaga
    30181 aattgtcata acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc
    30241 catggaaaac agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa
    30301 agacattgtc tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag
    30361 tttccaacat attttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt
    30421 caaagctgat cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc
    30481 cgtctacctt catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat
    30541 ttttcccttt agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat
    30601 aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc
    30661 ctgttttgat tccgtcataa atagtatacg catcgattcc tagatatttg acaatatatg
    30721 aacggtcaaa tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc
    30781 ctgaaaaaat aagccctgaa agacaggtaa cagcgatagc catcggtttg ccgacagggt
    30841 tgcttttgat aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga
    30901 tatccagcca gtagatgaca tcatggggct tcatcaaaga aaaggagctt cctgacaatc
    30961 cttgcgacac tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc
    31021 gataaaaaat aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa
    31081 tagctaacgc tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag
    31141 tcgcaattgg gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa
    31201 aatctgcata gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga
    31261 ttgctaaacg attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt
    31321 cacatttttt cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta
    31381 agattttttt attctttcta aagattattc cgtccacttt tcacagcata aaaccgctgc
    31441 gatgtaagcg attaaactaa tgaagagata aaagtcttgt ggtgaccgct tcactccacg
    31501 gtattcaaaa gccagtttct cggtattcgt acaaaaaaag aggctgtgac aaaattcttg
    31561 tcgcagctcc ttatttcttg atgcaggaca gctttttcac aacttcttcc ttaaacggtg
    31621 ttctcctgcc aactcctcgg ccacaagtca caatatttat aaaacatgag aagctgtttt
    31681 ttgaaattgt tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg
    31741 tgttcaatca gctgaccctc ggtattagat tatgtcagga cgctaatttt acaacctctt
    31801 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatata
    31861 aggagctatt ttttgaaatt gctccttgtg gctcagagtt aaacggcagg ctcaagacct
    31921 cttgatacac ggtgttcaat cagctgacct tcggtattag ctcaaaaaac ggcaaaatat
    31981 gaggaactat tttgccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    32041 tctgtttttc attgtccata ggattgatat tgtccttgtg atgactcatt cgtaataatt
    32101 gaggcttgat tttcttcact tcccgttatc gtttgtccac tgccagaatc tgaaggactt
    32161 gttcctgtcg actcgttttt ttgagaagag acagttgttt tcttgacttg ttcatttgtc
    32221 tttttccatt ccgctaaacg atcggcgacc acttttttgt ctactttttc agaataagcg
    32281 gtgatcactt ttccattttt caaacgggct actttgtcgc catcatgagt caaaagcaaa
    32341 acggtcaaat gcttgtcttt aaattgatat tgtctgagag aaatcgcact aagtacgtca
    32401 tcgatattaa taaattgatt ttcttctttt ccttttggca aataaatgtc caattcgcct
    32461 ttttcgtaat tggcagttat tttatactcc gcccaccaat aagttgagaa ttcttttttg
    32521 aaattatcta ctgattcttt gcttgctacg attttttctg tttctttctg agcaagatct
    32581 gctgtcattt ttcctgaaaa tgctgcggat aaaataagca gtaagaaaaa accgaataac
    32641 caaaagagaa gaaacggtct ttttttcctc atgctttcct ccactcctat ttaattttga
    32701 ctattctatt atatacaaaa aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa
    32761 taattgaact tctttttcac aaaatggtaa gaatggagaa tttcacgcat ttgctctcaa
    32821 ctattctttc ttaagcctca aatccgtttt tatctgatac gtctttgaac caatgacccg
    32881 attttttgat tgttcttttt ccatcttgtg ctaagtctac tgaaataaag ccatatcgat
    32941 tcttatatgc attggtccac gaccagttat ccatacatgt ccacatatga tagcctaaac
    33001 aattggcacc ttcttgaatg gcttgatgga catatttcag atgagattgg acaaattcga
    33061 tccgataatc atcgtggatc atgccatctt ctttgataaa tcgtgcttcg ttttctacac
    33121 ccataccgtt ttcagaaata aagcagtcga tatttccata atgatctcgt acattgatca
    33181 aaatatcata gattccttgt tcatagatct cccatccgcg ataaggattc attttacgac
    33241 caggcatttc ataattgtcg aaataatcct caggcattgg accgttttta gtatcgattg
    33301 gtgtttcttt tgcttttacc cggcgtggct ggtaataatt gatacctaaa agatctactg
    33361 tattttcttt gataaccgtc aaatcctctg catggatcac aggcatgaga tcaagttctt
    33421 ttactatctc aatcaattca cttggaaact cgccttttac agctggatca aggaatgaac
    33481 gattgaagaa cgcatctgct atttgcgctg ctttaacatc ttcagggttt gattcgtctc
    33541 ttggatagct tggtgtcaga ttcaagatga taccaataac accaccttgc tccatttcat
    33601 ggtaagattt gatggctaac gctgaagcca acgcttcatg atagccaact tggatcgctt
    33661 ttttcatatc tgactcttct ggataatgcc atcgatataa ataacctgct tcgactggaa
    33721 cgattggttc attatgagta aaccattttt ttactttcga accaaacaat tcaaaacaac
    33781 ttttggcata tttttcatag gcttcaaccg tttctcggtt caaccatccg cctttttctt
    33841 gcagatacat cggcatatcg aaatgataca gattcacaaa gggttcgata ccgttttcaa
    33901 tcaactcatc aaaataacga tgatagaaac gaacggcttc ttcgttgatt gttccagatt
    33961 catcaggtat caaacggctc cattggatag atgttcgaaa tgaattatgc ccagtctgtt
    34021 tcatcaactg tacatcctct tgatacttcg tataaacttg cgaggttttt tcaggtccta
    34081 cttgatcaaa gaacttttgc ggttcttttt tgtaccaata atcaaaaatg ttctctcctt
    34141 ttccatcgcc tgaaatcctt ccttctgttt gtgggccact tgctgcagaa ccccaccaaa
    34201 aatttgctgg aaattgatat tttttcatca aagcacctct ttttcttttt tagttcgttt
    34261 tagttgattc catgtatgat attttccatc acaatcactt tttcattttc ttcctcttct
    34321 tttttgattc gttttaacag ctgttctgct gctcgttccc cgattttatc tgttggtggc
    34381 gtgatcgtga ttttcggaag gatactcaga tcgtttttat ctaaataatc atagtgactg
    34441 aacgcaatat ccttcccgat tctgacacct tcttgattga aacattcgat ggcaccaatc
    34501 gacatattgt aattgctgac aaaaatagca gttgcccctt tttctaataa tgcttttgcc
    34561 ccgtaatagc cgctttttct agaatagtct cctgtaaaga cctctaattg gatcgatgga
    34621 tcattttgga ccgcttcttt ggctcctgct aaacgttctc ttgctacgta gtctgtttga
    34681 gtggcagcaa taaatccgat ttttttatgt ccttgttcac catgatgagc gatcactttt
    34741 gctacacttt ctcgatcatt cactaatatt gtatcaatac ctgctggtcc ttctggacaa
    34801 ttcaatgcga taactggaat actcattttt gtgagcattt caaatccaga ccactcttct
    34861 tcggataaga agacaatcag cccatcgatc acatgcttca tcaggtattc aattttttgt
    34921 tcaatttttt ctggctgatc ggcaaacccg cttaataata agctataccc attttcttca
    34981 gctactcttt cgatccctga gacaacttcc gacccaaaac ggctggaaat cccgtttatc
    35041 aaaagtccaa tagagaagct tcgattattc ttcaaccctt tagcaatgat gttttcttta
    35101 tagtccaacg ctgaaatcgc ttctgctatt tttttcatgt tcttttcctt cagttgctga
    35161 ccattcaagt aacgggaaac tgttcccact gaaacaccag ccatcttagc cacatcacgg
    35221 atcgatgcca tacattctca ctcctgccag ttacatttga tttctctttc ttgttgttcg
    35281 attatgatga ccgcattctt tgcatagtgt tgcttgtcat caatatccat ataactaaag
    35341 tcttcagtag agcaacttcc tctcactatt aattatactt aaacaagtat ttgaaccggt
    35401 tcatttttaa tatagcattt tttt
//
LOCUS       DAZUMB010000014        35037 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000014 DAZUMB010000000
VERSION     DAZUMB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.014"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..631
                     /locus_tag="ACG80L_000588"
     CDS             179..631
                     /locus_tag="ACG80L_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5022872.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(755..1606)
                     /locus_tag="ACG80L_000589"
     CDS             complement(755..1606)
                     /locus_tag="ACG80L_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5022873.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1619..2404)
                     /locus_tag="ACG80L_000590"
     CDS             complement(1619..2404)
                     /locus_tag="ACG80L_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5022874.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(2756..3697)
                     /gene="ribF"
                     /locus_tag="ACG80L_000591"
     CDS             complement(2756..3697)
                     /gene="ribF"
                     /locus_tag="ACG80L_000591"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS5022875.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(3701..4624)
                     /gene="truB"
                     /locus_tag="ACG80L_000592"
     CDS             complement(3701..4624)
                     /gene="truB"
                     /locus_tag="ACG80L_000592"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS5022876.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(4992..7354)
                     /locus_tag="ACG80L_000593"
                     /pseudo
     CDS             complement(4992..7354)
                     /locus_tag="ACG80L_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            7519..7975
                     /locus_tag="ACG80L_000594"
                     /pseudo
     CDS             7519..7975
                     /locus_tag="ACG80L_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(8132..8479)
                     /gene="rbfA"
                     /locus_tag="ACG80L_000595"
     CDS             complement(8132..8479)
                     /gene="rbfA"
                     /locus_tag="ACG80L_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS5022877.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(8506..10812)
                     /gene="infB"
                     /locus_tag="ACG80L_000596"
     CDS             complement(8506..10812)
                     /gene="infB"
                     /locus_tag="ACG80L_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS5022878.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(10825..11136)
                     /locus_tag="ACG80L_000597"
     CDS             complement(10825..11136)
                     /locus_tag="ACG80L_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS5022879.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(11133..11426)
                     /locus_tag="ACG80L_000598"
     CDS             complement(11133..11426)
                     /locus_tag="ACG80L_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS5022880.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(11448..12623)
                     /gene="nusA"
                     /locus_tag="ACG80L_000599"
     CDS             complement(11448..12623)
                     /gene="nusA"
                     /locus_tag="ACG80L_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS5022881.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(12646..13119)
                     /gene="rimP"
                     /locus_tag="ACG80L_000600"
     CDS             complement(12646..13119)
                     /gene="rimP"
                     /locus_tag="ACG80L_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS5022882.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(13264..17622)
                     /locus_tag="ACG80L_000601"
     CDS             complement(13264..17622)
                     /locus_tag="ACG80L_000601"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS5022883.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(17834..19543)
                     /locus_tag="ACG80L_000602"
     CDS             complement(17834..19543)
                     /locus_tag="ACG80L_000602"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS5022884.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(19611..20879)
                     /gene="rseP"
                     /locus_tag="ACG80L_000603"
     CDS             complement(19611..20879)
                     /gene="rseP"
                     /locus_tag="ACG80L_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS5022885.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(21040..21840)
                     /locus_tag="ACG80L_000604"
     CDS             complement(21040..21840)
                     /locus_tag="ACG80L_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS5022886.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(21837..22649)
                     /locus_tag="ACG80L_000605"
     CDS             complement(21837..22649)
                     /locus_tag="ACG80L_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS5022887.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(22844..23401)
                     /gene="frr"
                     /locus_tag="ACG80L_000606"
     CDS             complement(22844..23401)
                     /gene="frr"
                     /locus_tag="ACG80L_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS5022888.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(23404..24126)
                     /gene="pyrH"
                     /locus_tag="ACG80L_000607"
     CDS             complement(23404..24126)
                     /gene="pyrH"
                     /locus_tag="ACG80L_000607"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS5022889.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(24262..25143)
                     /gene="tsf"
                     /locus_tag="ACG80L_000608"
     CDS             complement(24262..25143)
                     /gene="tsf"
                     /locus_tag="ACG80L_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS5022890.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(25242..26024)
                     /gene="rpsB"
                     /locus_tag="ACG80L_000609"
     CDS             complement(25242..26024)
                     /gene="rpsB"
                     /locus_tag="ACG80L_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS5022891.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            26383..26862
                     /locus_tag="ACG80L_000610"
     CDS             26383..26862
                     /locus_tag="ACG80L_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5022892.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            27079..28170
                     /gene="phnW"
                     /locus_tag="ACG80L_000611"
     CDS             27079..28170
                     /gene="phnW"
                     /locus_tag="ACG80L_000611"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS5022893.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            28163..28291
                     /locus_tag="ACG80L_000612"
     CDS             28163..28291
                     /locus_tag="ACG80L_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS5022894.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            28295..28840
                     /locus_tag="ACG80L_000613"
     CDS             28295..28840
                     /locus_tag="ACG80L_000613"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS5022895.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(29294..30985)
                     /gene="argS"
                     /locus_tag="ACG80L_000614"
     CDS             complement(29294..30985)
                     /gene="argS"
                     /locus_tag="ACG80L_000614"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS5022896.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(31405..32352)
                     /gene="arcC"
                     /locus_tag="ACG80L_000615"
     CDS             complement(31405..32352)
                     /gene="arcC"
                     /locus_tag="ACG80L_000615"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS5022897.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(32467..33486)
                     /gene="argF"
                     /locus_tag="ACG80L_000616"
     CDS             complement(32467..33486)
                     /gene="argF"
                     /locus_tag="ACG80L_000616"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS5022898.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(33577..34806)
                     /gene="arcA"
                     /locus_tag="ACG80L_000617"
     CDS             complement(33577..34806)
                     /gene="arcA"
                     /locus_tag="ACG80L_000617"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS5022899.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         9469 a         7500 c         6173 g        11895 t
ORIGIN      
        1 caggtatatt taaatataat atcactttag gagagaacga atttctttag agaaataaaa
       61 gttagaattt tctgacttga tttttacaat gaccttaatt aaaatcgttc tttctacatt
      121 tcgtgattaa gcaaatatca actaagtcag tagaaccatt cacataggag ggtgaacatt
      181 gaaagatttt tactcagtaa atgaattagc cgaacaatta ggtgttacta cacgttctat
      241 cagaaattat cttcatgaag gaaaactaaa aggaacaaag gttggcgggc aatggaaatt
      301 ttctgaacgt aatctttttg agttcctata cggagatcaa gcagaggaag cagcaaaaga
      361 aatgcagcga ttcatgctaa atgcaccaat cacgatgcgt ttcaaccttc aatatcgtga
      421 ttttacagca atcaatcaat tccgagaaca attagtccag taccataacg atgtttacgc
      481 aaataaaaaa gaccgtcttt tacaatacga tctttataaa gacaatcatg cggagatact
      541 aatcggtggt aacttcaatt atgtcgtaaa ctttagtcaa tggatcaatg aaaaattatt
      601 gatccagaca gatatttccc ttgtatctta aacaattaaa aagcgctaga aattccagag
      661 atgttggaaa ttctagcgct atttttttat aagtacttac tgtttggttc aaacaaatat
      721 ttttttaata aatgaaattt taccatcttt atcctcaaat aaagaccgtg accatgcttc
      781 ctactacgat cagcaataat ccgataatcg ttaatttttt ttcttttggt gtcttatttt
      841 catgcaggat gtacataccg cctaatgtag agataactac gtttagctgt gtaatgatat
      901 atgcagtagc aaccccattt tcatttgctg aaaaaatata agccaatgct gacacactaa
      961 agatggctcc aacgatcgtt gctttccaac tcttgtcttc agaaaatgcg gttggtttac
     1021 ggaaaatcac ataaatcact gcccctaaaa agacaccgat catttgcgga aagaaaatgg
     1081 aaagtccgct tgcatcaact agtttaggca atgcactgta tacccaataa ccaatacttg
     1141 ttgatgctaa tagactaatt ccttttgtga taccgccttt ttttccagga tcatcactga
     1201 ttgccgttaa agcagaacca aatacaagaa gaatgattgc tgctgctcca atgattttcc
     1261 ccatacttcc agaccattct ccaaaagcga agacaccaat caacgaggtt ccgactaact
     1321 gtaatgcagt cgaaattggc atcgttttag aaacaccaat caagttgaaa gatgtatact
     1381 gtcctgtttg tccaataacc caaaatgcac cagaaagtaa actaatcaaa aatgtagtca
     1441 ctgaaatacc agctggagaa gcgaataatt gaacgagtaa gccgacaagc accgctccaa
     1501 taccagttcc taatatctca tttgttggtc tgccaccaat ctttttcaaa atcagtggct
     1561 ggatccccca gccaactgcc gggatcaatg caatcaataa acccattttt attcaccctt
     1621 atccttttcc accttggaaa gaaggatata aagtcatccc accatctgca aatagtgtaa
     1681 tacctgtaac ataactggat tcgtctgaag ccaaccatgc agctgtcgcc gctatttctt
     1741 ttggttcacc tacgcgtcct agtgggatca tactttccaa gagttctttt tgttcaggat
     1801 catcaaattt tgccgcatta ataggtgtac ggatagcacc agggcctata ctattgattc
     1861 tgattccctt cggtgcatac tccattgcta ccgtttcggt aaataatttt accccgcctt
     1921 ttgaagcagc gtaatgagca aacgttggcc atgggatctg ttcatgaaca gaagacatat
     1981 tgatgatatt tcctttttta ttattctctg taaagtaatt taaagccgct ttcgttccga
     2041 taaaaacgcc agtcaaattg acttttaaca ctctttccca atcttcaagg ctaagttcat
     2101 gggttggatg ctgattttcc atcccagcat tattaaccca aacgtccagt gtcccaaaat
     2161 tttctaaagc cgtatcaagt aaacgctgta ttccttcttc agaactgaca tctgcttgta
     2221 cagagactgc ttttccgcct gcctcttcga ttgctttgac tgctttttct gctccttctt
     2281 tatccgagtt atagttgaca actacattca ttttttcttt cccgaatcgt tgagcaatcg
     2341 ctgttccaat cccctttgag ccaccagtta ctactgcgac tttgttttct aattccttgt
     2401 acatagtacc cactcctttt tcatacctaa acctgttatt acggataact tttatttccg
     2461 ccttaaagtg agtgtacacc aagatatata gtctaaagca aatgatacgc tctagcaatc
     2521 gttttcatga attgctagtg tccttttatt tctataaaga aagaggttgt gacagaagtg
     2581 gtcagcttca agaaatttcg gcttatttcc gaagaagcta cttctgaaaa accatttatt
     2641 tggaaaaaaa ggctgtgaca agacttttgt cacagcccct ttcctgggct tatttatcac
     2701 ccattttatt gtaattgcta gttatacata aacaagagat tttatgcata atgttttatt
     2761 gctgagaaaa atattcagct gttgcttgtt catcctcctt gagttgttca atcaattcct
     2821 ctacactaga aaatttcact tgatctctta aaaattgatt ccagcgtacc gcgacatgtt
     2881 caccatagat atcttgatta aagtccaaaa tatacaactc tacagtcagc tcccgtcctt
     2941 caccgaatgt atcattatgt ccaattgaac ccatggctgg ataccatgtt tccccaattt
     3001 ttatttccga cacataaaca ccttctttag gcaaatgaac cgtgctcttt actttgacat
     3061 ttgctgtagg gaatcctagt agtctgcctc gtgcatcgcc atggacaact accccttcta
     3121 cctcatatgt gcaacctaat agttttgctg cttcttttac ttgtccgctg tctagtaatc
     3181 cacgaatacg ggtagagctg attttttcac cttgttcttc ttctttagaa actgtgacga
     3241 ttttgaatct tcctttggca tattcaggaa aatgagcgac atctgcaatg tcttttggtc
     3301 catacgtata atcaaaaccg gaaacagcat attttgcatg taaccctacg atgtattgat
     3361 caacaaattc ctgcggtgct aaatgagcaa aagaagaagt aaaatcaact tcatataaat
     3421 aatctatacc caaagcggcc attttttctt ctttttgctt aaggctagtc aaatatttca
     3481 cgctgctagg atcgatcttt ttaaagacaa tagatggatg ctgattgaaa gtcatcaatg
     3541 ctaatttcaa tccttctttt tgtgcaattt ccatacctgt ctttatgact ttctggtgtc
     3601 cgatatgcac gccgtcgaaa aatcccagca ctaaaacgac atcgtcttct ggaatttgtt
     3661 ctgattggta aggatgtctg atcttgatga tattcattcg ttattcctca tttctcaata
     3721 ctttactagg ttttaatttg tttttttctt tcgggtttgg ctgataaata ctgacaactt
     3781 ttcctttata aaacaaagca atttcttctg acggcatttc tgggagcgca aatacagtat
     3841 aatcaagtct cataccgttt tttactttct gccatacttc atcagtaata tctactcgtt
     3901 tgaaccgttc cactcctttt tctattggca gcaaacaatc atcgattgtc ccatttttca
     3961 cagcttgagc tacttcttct aatgtcagag catccttctg ggacataccg gcacttgctg
     4021 ttcgggtcag atcagacatg tgcgcagggt aaccaagttt cttgcctgta tctacagcta
     4081 atgtgcggac ataggttcct ttcccgcatt ctacttcgaa gcgccaagaa atggttcctg
     4141 tcacttcgtc tcttgtcaat tcacttgtcc ggataaaacg gtaaatctga gcctttctaa
     4201 caggacgctc cactgtttca ttattgcggg catattcata taaccgctta ccgttgactt
     4261 ttaccgctga gtacattggt ggtatctggg tgatctcacc aataaatgaa gccatcactt
     4321 catctatttt tttttcagat aaagacatat ctactggttt tgtttctacc cgttctccgg
     4381 atcgatcttc cgtcgttgta ctgaaaccta gagttatctc gcctacatag attttcccag
     4441 aatctgtcaa atattcgata acttttgtgg cttttccaat acaaatgggc aacacaccgt
     4501 ctacgtctgg gtcaagcgta cccccatgtc cgattttttt cgtatgtaag atttttctta
     4561 atttgaatac gcaatcatga cttgtcattc cgcgttcttt ccataaaggt aataacccgt
     4621 ccatgtcttt tctccatttc atttttgatt taactgaatt attataacac attgcatcaa
     4681 ttactgacga atttgacatc ataatgaacg gtttttattt ttcggcaata aaaacgtaac
     4741 aaaattcatg ctcacgtaaa tagaaatcat gttatgcttt tttaaaggag ggaatagaat
     4801 ggaagatttt ttctttgcac catttgattg gatttatggg tggcttacag gtaaatagtt
     4861 tggttttcac ttcccaaaaa cgaaaaaaaa gactggccat tcttccagtc ttttttccgt
     4921 tttttttatt tattttttca gtttcttaat caatgtaaaa aattctattt tgcgggatcg
     4981 attttatcat tttattggtc aagatttttc catttccaat ccaatacttc tggcagatca
     5041 ataccatttt cacgaatata gttttgatgt tcttgtaatt tctgatccat ttttttcgca
     5101 aaatcttctg cttgacttcc tttgattttt aaagctgcgt cttttgccaa atgataacga
     5161 tcaagttcat ttactacacg catgtcaaat ggtgtcgtga tatctccatt ttctttgtag
     5221 ccatgaatca ttaaatgatg gttatgacga tcaaagaaaa tcgtgcggac caatgtttca
     5281 taaccgtgga aagcaaataa gatcggtttg tcttttgtaa aatattgatc aaattcttca
     5341 tcgctcaacc cacgcggatc ttgacttgga tgacgtaatt tcaataaatc tacgacattg
     5401 atgaaccgaa tttttagttt tgggaattct tccagcagca aagttacagc tgctaatgct
     5461 tctaagttag attccgttcc tgcagcagca atgaccaatt caggttcctc ctcttttgct
     5521 gtcgaagccc aatcaatgat tttcaagcct tcattcacta agacggctgc ttcttctgct
     5581 gagtaaaatt gagcacgcgg atgtttggaa gagatgatca aattgatttt ttctgacgag
     5641 cggaatgctt tatccatgac tgcgagcaat gtatttgcat ccgcaggcag atattcacgg
     5701 atgaactccg ccttcttttc tgccaaatgt gtcaatattc ctggatcttg gtggctatat
     5761 ccattgtgat cttgctggaa gactgttgag cttgcaatga gatttagcga aggataatca
     5821 tttcgccaag aaaggtcatg tgatttccgc atccatttga agtgctgagt caacatagag
     5881 tcaaccactc gcaagaatga ttcgtaactt gcaaaaaacc atgccgtcca gtcagaacgt
     5941 acccttctaa gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag
     6001 atggcgaaag ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg
     6061 ttttgaaaac ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat
     6121 tttctggatt tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg
     6181 ctttaacgga gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa
     6241 gcggtcttgg atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag
     6301 taggcgcgat ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg
     6361 gttcatagga tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta
     6421 caggaatcgg tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt
     6481 ttggaccggt ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga
     6541 catctgttgc tgtcttcagg cgagcttctt tttggatgga tttaattttc tcgattgctt
     6601 gatccatttt ttcagccata acaggattta atttttctga atcatttcct tcaataaaga
     6661 aaggttccca gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca
     6721 agatcgttgg gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat
     6781 ctatcactgg attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg
     6841 cttcaccatc accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga
     6901 ctgcatgtga aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg
     6961 gtgctgcatg agaagcaatg cctccaggaa atgaaaatcg tttgaataat ttttgcattc
     7021 ctgtctcatc ttccgttact tctggataga tctctgtgta acttccatct aaataagcat
     7081 tcgataccat gacttgtccg ccgtgtcctg gcccttcaat ataaaacatg ttcaaatcat
     7141 atttattgat gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc
     7201 aatgaccgat tggatgtttt ttgacatctt cgggtttcag tgtacgtctc agtaaggggt
     7261 tatctttaag ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca
     7321 ttttatcaaa gtattcttta ctagaataat ccatacataa cactcctttt cattattact
     7381 cttattctag cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg
     7441 gattttatat ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga
     7501 cgttttttac attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt
     7561 tcgctcctca tcatgcataa tttgctgaat gacgctgggc cagttaacac attgctggaa
     7621 aactggggct ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg
     7681 ttatctttgt caatatgtga attggtatcc cagctactat gctaatttct acagggatta
     7741 tccaaaattt ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa
     7801 cgtgattttt cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc
     7861 cactgatctt ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc
     7921 ttccgttatc ggtatcttga tctttatcta tatgcaaaaa tttatatact actagaaaaa
     7981 agaaagtcga attttttact tttctttttc tcttttttgg ttagctgaca cgccgcaaga
     8041 ctaaagtcat ggaactctta ggtaatacgt cttaatgaaa acaaaaaaat cccgcaaccc
     8101 ctgatttcac aggatttgcg ggagtttttg attagtcttt gttcaagtcg cgaagcaatt
     8161 catcgatatg gttgccatag acaactgatt catcaagttc aaagaacaat tcaggtgtct
     8221 tataaatact tagattgtgc cctaactctc gacgaatcaa tcctgatgct ttattcaatc
     8281 catcttgtgc tgttttctta tctgatgcta agtcagataa aatgctgtaa tagatcgttg
     8341 cttgctgtaa gtcccccgta actttcacat ctgtgatcgt tacgttctcc acacgagggt
     8401 cacggacctt tttgcgcaaa atatcattga cttcttttaa gatttcttga ccgactcggc
     8461 ggtcacggta gttagccata atgattggcc tccttttatc agtttttagt catttttgac
     8521 ttcttccatg ataaatcctt caattacgtc atctactttg atgtcattga atttttcaat
     8581 cattgcacca cattcataac ccattttgac ttctttcacg tcatctttga atcgtttcag
     8641 acttgcaagt tggccttcat agattacgat tccgtcacgg attacacgga caccgctatc
     8701 tctacgaata tatccttctg ttacataggc acctgcaatc gtaccaactt tagaaacttt
     8761 gaaggtttca cgaacgatca tttgaccggt gattttttct tcaaattcag ggtctagcat
     8821 ccctttcatc gcagtttcga tttcttcgat ggctttatag atgatacggt gaagacgaat
     8881 atcgacttct tcagtatcag cttgcacttt tgcctgcggt gtaggacgaa cgttgaaacc
     8941 gatgataata gcgttgctgg ctgctgctaa ggttacatca ctttcattga ttgctccgac
     9001 agctgaatgg acgattttta cacggacacc ttccacatca atcttcttca atgatgcagc
     9061 cagtgcttct gctgaacctt gtacatctgc tttgatgatt acgttaactt ctttcaattc
     9121 gccttctttc aggctttcaa ataagttatc caaagtcaca cggcttgtgg cagcacggtt
     9181 ttctaataat gcacgtttcg cgcgttcttc acccgctgca cgtgctgtct tttcgtcttc
     9241 gaagactacg aaacggtcac ctgcttgagg tacatcattc aagccagtga tttctactgg
     9301 cgttgcaggt cctgcttctt tatcgcgacg gccaagatcg tttgtcatta cacggacacg
     9361 tccgtaagta ttaccgacaa cgattgggtc accaacatga agtgttcctt cttggacaag
     9421 taaggttgac acaggacctt tacctttatc aagacgtgct tcgatcactg ttccgattgc
     9481 gcgttgtgtt ggatctgctt ttaaatcttc tacttctgcg actaacagga tcatttccag
     9541 caattcttcg atgttttggc cgaatttcgc tgagatctca acaaagatcg tttctccacc
     9601 ccatgcttca ggaattaact catactcact taattcttgc ataacatgtt gagggtttgc
     9661 acctggttta tcaattttat ttacagcgac gatgattgga acgcctgcag ctttggcatg
     9721 gttgattgct tcaactgttt gtggcatgac gccatcatct gcagcaacaa ctaagatcgt
     9781 gatatctgtg atacttgctc cacgtgcacg catacttgta aaggccgcat gtcctggtgt
     9841 atccaagaaa gtgattggtt taccatcaat atcgatctgg taggcaccga tatgctgtgt
     9901 gattccgccg gcttcgccgc ttgttacacg tgaatggcgt aatgtatcca ataaagttgt
     9961 tttcccatgg tctacgtgtc ccataatcgt tacgactggc ggacgtgaga ctaatttatc
    10021 tggatcgact tcttctgcct cgaagaattt atcgatatca gcaatgtcaa cttggacttt
    10081 ttcttgtggt tccatgccat agtccgtcgc taacagttca atcgtatctt tatccagtgc
    10141 ttggttttga ttgaccatga caccaagcat aaataatttt ttaatgattt ctgctggctc
    10201 acgatgaatt tttttcgcaa tatctgctac gttcatacct tcagtatact ctaatacatc
    10261 tggcaattca cggaatttgc gtggtggtac agctggttta gttgattgtt gctgtttgcc
    10321 ttttttacct tttttattat aacgattacg gttattatta ttaaatttgc cgcgattgtt
    10381 attgtttggt ttgttgttat tgttagtacg gttttgattg ccgccttggc tcgttccttg
    10441 acgattttgg ctgctggtat ttcccgtatt attttggttt gttgttggtt tttttccttg
    10501 attcacttga ccgctccctt gaccgcgatc ttgataactg cggttatttt tatttttttg
    10561 ttgattgttc gttgtttctt ttttacgttg atcgttcggc tgttgattgg ctggttttct
    10621 gcttgcttgt tgtgcagctt gttgtttcgt gcttgggttt ttgaacgctt gttgcaattt
    10681 cttctcatca ttgctagtaa tcgcacccat atggtttttc acatcgatgc caagttgttg
    10741 tgctttttcc acgacgtctt tactagattt tttgagttct tttgctaatt catagattct
    10801 tttgttgccc atgcaatcac cttcctaacc ttcaattagc tccttggtct tcttcgcaaa
    10861 tccggcatcc attattccga ataccttgcg aggttttccg atcatctgcg tgatttctgc
    10921 ttcagaaaat agctcaaagc aaggaacttc ataatatgaa ctcttatctt ttatcttttt
    10981 tcttgtattc tcgcttgcat cggttgccac aaacaccaat tttgcttttt gtttgcggat
    11041 ttcagcaatg gtcagttctt cacccgtgat tagttttccc gcgcgcatcg ctagaccgat
    11101 gagattcatg gctttttgcc gattaactgt attcattttc caaataactc tttacgtgct
    11161 ttttggtgag tgacatagtc aagaagttct tgataaaact catctgttaa tgttgtttcg
    11221 agtactcgat cgagaatttt tttatcccaa gcttgttgta cttcttccgg ctcaagtgag
    11281 acatacgctc ctcgtccagg catttttcca gttggatcga tcgatacttc cccttctttt
    11341 gaacgggtga ttcggaccat ctctttttta ggtttcattt cacctgaaac cacggatttg
    11401 cgtaaaggaa ttttcctttg tttcatctct ttgcctcctt actaaagtta agcttctggt
    11461 ttttcttcga ccgtttcatt gttgaatgca attgtttcat attcatcgtc tgttaaatct
    11521 gactgaacga ttgcttcatc atgcagttct tctgtttctt tttgttgttc ttgattttca
    11581 taaaattccg tcatgtcaga ttctgatttg atatcgattt tatggttagt caatttagca
    11641 gctaaacgtg cgttttgtcc gcgtttaccg attgctagcg ataattggta gtctggaacg
    11701 acaactgtgc aggcctttgg atttgtctca tcgaagatca catccatgac ttgtgcagga
    11761 ttcaatgcat ttgcaatgaa tacagctgga tcttcattcc attcgacgat atccatgttt
    11821 tcgcctttta attcattgac gattgcttga actcgttgtc cctttggccc gacacatgta
    11881 ccgactgcat cgatatttgg atcatttgag cgaactgcca cttttgaacg atcgccagct
    11941 tcacgggcga tgctgacaat ttcaacgatt ccatcataga cttctggtac ttcttgctca
    12001 aataagcggc gcaataggtc tggatggctg cggctaacaa atacttgcgg acctttagac
    12061 gtattttcta ctcgggaaac ataaactttg attcgatcat gtggttgata aaattcattt
    12121 ggcatttgat cttgtttaga aagaactgcc tcaattttac caagattgac ataaatataa
    12181 cgtttatcct ggcgttcgac gattccttgc atgatgtctt tttcataagc actgaattcg
    12241 ttataaataa tcgttctttc tgcttcacgt acacgctgta aaataacttg tttagccgtt
    12301 tgcgcagcga tccgcccaaa atcttttggt gttacttcaa aacggatcgt atcaccgatc
    12361 tcatatgctg gattgattaa aagtgcatct ttcaatgaga cttccaactg tgaatccatc
    12421 acttcttccg ttacttcttt taccgcatag acatggatct ttcctttttt ctgatcaaat
    12481 tccacttcga cgttttgtgc ttggccatag tgacgtttgt atgcagaaac caatgctgct
    12541 tctaaagcat cgatcacgat ttcttttgag atcccttttt cagcttctaa agcgtctaac
    12601 gcatttaaca tttctttact cattttttct gattcctctc cgtgattaaa attggatagc
    12661 taagcgagct tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat
    12721 gcgtattttc aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt
    12781 ttcgccatct actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata
    12841 gtcgctttct tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc
    12901 ttgaggaatc ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa
    12961 ggcgcattct tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct
    13021 ttttccttct tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg
    13081 tgtcaccatt tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaaa
    13141 ttaagtgcgt aattccatta aaaagagtga gcgaaaaatc cgctcactca cagttagtat
    13201 cattacctat aagaactata ccatactctg cttaaaatta caagcagaca ggaaaacaga
    13261 tgcttatagc atatcgaata acgataattg attttcatct ggtagatctt tcaatacgcc
    13321 attgtcgttc atatattcga tcaatgtttt tgaaacttta ccgcgggtgg caagatcttc
    13381 tttggataaa aatggaccgt cttttcttgc ttcaacgatt tgttttgcca cgttggttcc
    13441 caaacttggg acagctctaa atggtgcgat caacgtatca ccttcaataa caaagttaac
    13501 ggcatccgat ttatacaaat caatcattcc aaatttgaat cctcgttcca gcatttcatt
    13561 ggctaactcc agcactgtca gttgattctt ttcttttacg gaagcatcta atcctttgtc
    13621 cgtgatttct ttcattgctt gtttcacagc atcttttcct ttgcacatgg agactagatc
    13681 aaagtcgtct gcacgtacag aaaagtatgc acagtaatac aaaataggga aataaacctt
    13741 gaaataagcg acacgaagag ccatcaatac gtaagctgca gcatgggctt tcggaaacat
    13801 atatttgatt tttgaacaag aatcgatata ccagtcgggc acattatttt ctttcatcgc
    13861 cgatagatag gtttctcgca attcatcagg gatcttgttc cattgtcctt tacgaacagt
    13921 ttccatgatt ttaaatgcca tcccgctatc taatccagca tggatcaaat aaaccataat
    13981 atcatcacga cagccgatta cttccgccag tgtcgcatct cctcgtcgaa tcaattcttc
    14041 cgcatttcct aaccatacgt ctgtaccatg agatagtccg gaaatttgca gtagttctgc
    14101 aaaagttgta ggatgcgttt cttctagcat ccctcgaaca aaacgagtgc cgaattctgg
    14161 aatacctaat gtccctgttt tggaataaat ttgttcctga cttactccaa gtacttcagg
    14221 accggcaaat atccgcatca cttccgggtc atccgtaggg atcgtttgtg gatcgatccc
    14281 tgataaatct tgaagcatcc gaataacagt tgggtcatca tgccctagaa tatctaattt
    14341 caatacatta tcgtgaatcg agtgaaaatc aaaatgagtc gttttccatt ccgaattttg
    14401 atcgtctgct ggatattgga tcggcgtaaa atcatagaca tccatataat cgggaataac
    14461 gatgatcccc cctggatgct ggccggttgt ccgtttgact ccagtagcgc cttttgctaa
    14521 tctgtcgact tctgcacttc gaaactgcag attattgtcc ctttcgtatc cttttacata
    14581 gccatatgct gttttgtcgg cgactgtacc aatcgttccg gcacggtaaa catagtcttc
    14641 gccaaacaat actttcgtat agttatgggc ttctgcttga taatcacctg agaagttcaa
    14701 atcaatatcg ggcactttgt ctccatggaa tcctaaaaat gtctcaaatg gaatatcatg
    14761 accgtctttg tttaagcgtg caccacattt tggacattgt ttttctggca tgtcaaatcc
    14821 agaaccatac gtaccatctt caaaaaactc ggaatattgg cactctggac aataataatg
    14881 aggggctaac ggattaactt cagtgattcc tgtcatcgtc gcaacaaagc tagagccaac
    14941 agagccacgt gaacctacta gatagccatc ttcattactt ttatgcacta gtttttgggc
    15001 gatcagataa ataaccgaga agccattacc attaatagaa ttcaactctt tttttaatcg
    15061 tttttgaatg atctcaggta acggatctcc gtacatttgt tttgcttttg tgtaactcaa
    15121 ttctgatatt tcatcttctg aacctggaat cttcggtgta taaagttcat ctttgactgg
    15181 aatcacttct tcgcagatat ctgcgatttt attcgtattt tctactacga tctctttcgc
    15241 tgtttcttcc cctaaaaagt gaaatgcagt tagcatctca tcggttgttc ggaaatggac
    15301 atccggcaaa ctatgacgat tcaacggatt cgctccgccc atagaattga tcaaaatttt
    15361 tcggtaaatg gcatcttctt cgttcagata atgtacatta cctgttgcaa caacgatttt
    15421 atccaaactt tttccgattt ctaccaaatt ttggataatt tcttccaaat catgttcatt
    15481 ttttaccaat tcttgctcga tcaaaggcgc atagaccgct tttggcataa cttcaatata
    15541 atcgtagaat tttgctcggt ttcttgcctc ttctacccct ttttgcatca tggcttcgaa
    15601 tatttctccc ttatcacaag ctgagccgat caatagattt tcacgcatct ttttcaattg
    15661 ggagcgtggt attcgtggga cgcgttcgaa gtattcgaca ttcgacattg aaatcagttt
    15721 gaacaaatct ttcagtcctg cctgattttt tgctaaaatc gttacatgga aaggtcgagc
    15781 ccttttgtaa gaatctcctt ctccgatatg ctcatttagc tgatcatgat aaagcatgtt
    15841 atgattgtcc attgcctctt tcacaaagat ccatgctaag tgaccagttg cttcagcatc
    15901 atagattgct cggtgatgct gttccaagct gacaccaaat tttttagata acacacctaa
    15961 tccgaaacgt ttgaattgag gatacagata tctagctaat tccaacgtat caatgactgg
    16021 attggccgct tcaggaatac cataccttgc gtagctagta ttcaagaacc ccatatcaaa
    16081 agccgcgttg tgggctacca aaattgtatc ttttgaaaat tcaaggaaca tccgcaatac
    16141 ttcttcttcc gactttgatc ctcgaaccat tccatccgtg ataccagtca gatctaccgt
    16201 tgttcgtgac aaaggatgtc ctggatcgat aaattcatca aagctttcga tcacatttcc
    16261 tttatacatt ttgactgcgg ctagctcgat gatcgtgtca tatactgcag acaatcctgt
    16321 cgtttccacg tcaaatacga catatgtggc ttcactcaat gcttcatgtg catcattata
    16381 agcaattggg acaccatcat ctacgacatt cgcttccacg ccgtataaga ttttcacacc
    16441 tgcttttttt ccagcactat gtgcttccgg aaaggcttgc gcaccaccgt ggtctgtaat
    16501 ggcgatagct cggtgtcccc atttccctgc ttgtgccaca agatcagaga tgctgtttgt
    16561 tgcatccatc gtactcatat tgctatgaac atgaagctct actcgttttt ccccttcagg
    16621 agcataatct ttgcgaggtg tatgtttgac ttctatgata tcttgcgcat tcatgaccaa
    16681 atcacggaca aacgtatctt cttgaatact tcctcgtacc tttaaccagc taccgacact
    16741 gatcgcatca aatacttgtt catctttttc gccgttcgag aattttttga cgataaatga
    16801 tgaagtataa tctgtgattt tcaaagtcaa gatcttgcgt tttgaacgta gttctctgac
    16861 ttctttgtcg aatacgtacc cctcaagtgt gactcttcgt tcttcttcaa cgatactgat
    16921 cattggtgtc gttggttcat cggctggaat atttcgtcct agctggatgt ggatgggtcc
    16981 ttcaagagct ggcgattgct cttttctttc ctttttcttt tgttcgtgga caactagact
    17041 ttcagctgct tgcttcataa aggcttctgc ctgttcttgt ttgcgttctt caaaaagttt
    17101 caatactcgt tccgcttgct gctcatccac ttctggttcg atacgaaatt ttgggaatcc
    17161 gtaactgacg aacaactctt ctactaatgg caagtattgt tgcttcaaat aagaaatagc
    17221 tcctgtactg tcgattggca agatgacttt acgatctttt atcacaggta cctgcgtttt
    17281 caatacttgc tgtgctagtg gtgtatcgca ttgctgattt tccaaagctt gggcccagta
    17341 atcttgcaag agctgttcat cgaatgtctg gtcatctgct aagatattca gtttgacttg
    17401 tgcaatatct ttaaaagcga tagtcagatg ctgagtcaat gaccgatata gcataatagg
    17461 caaaattttg gaaaaatgca gcgtaaattc ccataaacgc gatttacgat ggaccactac
    17521 actatccatt tcaccagcag taagcagtgg atgatttttt tctgcttcct ctagttggat
    17581 ttgttccaac agtttttcaa ataaatctcg tttttctgac aaagatatac gccctttctg
    17641 atgagaaaca atgcaattga tcgcatctta tcctcttctt tttcagtacc ttttagtata
    17701 ctcgtttttt ctgagtttta cattaaaaaa ttggaactgc gattcaaatc gcagttccaa
    17761 ttttaaaagc gaatcatcag attgtcaaaa acagctgatt cagctatttt tattttctgt
    17821 gttttaacgc ttattattcg ccttggttca agaggattgg taaagtattc accaattctt
    17881 ctttacgtac ttcaaccatt tcgccagttt tcttgatttt cacttctacg attccatcca
    17941 ctgctttttt accgacagtg attctgattg gacagccaat caaatcagaa tccgcgaatt
    18001 tcactcctgc gcgttcattt ctgtcatcaa ccagtacttc atagccagct tttgtcatcg
    18061 cttcttctag ttcattcgtc aggtttgttt ggtattcgtc ttttagattc atttggacga
    18121 tatgcaaatc aaatggcgca attccttttg gccagttgat tccttgttca tctgaatgtt
    18181 gttctacgat tgctgataat aaacgactta caccgatacc ataacagccc ataatcacaa
    18241 atttttctcg tccattttcg tctagtacag ttgctcccat agattcactg taacgagtgc
    18301 cgattttgaa gatatgtccg atttcgattc ctcgagtaaa ggcaagaaca ccattgttgt
    18361 ctggtgaagg atctccttct tgtacaaaac gaagatcttc ataagaaagt acagtgaaat
    18421 cgcgttctgg attcacattt ttatagtgga atcctgtttc gttcgctcca gtaatggcat
    18481 tcgctaaatc ttggacataa cggtctgcat atacttttac gtcatctgaa acaccgactg
    18541 gtccgacaga accaaactca gcacctagat atttttgtgc ttcttcttct gttgcttctt
    18601 ctaaaaagtc agcagataaa aagtttttca gttttacgtc attgacttca tggtctccgc
    18661 gtaccaaaac aaggacaggt tgttcatccg caatgaacaa tacagacttg atgatttttt
    18721 gaggttcaac agaaaagaaa tctgctactt cgtcaattga ttttacgtta ggtgtagcta
    18781 ctttctccaa ttcaagctgt gtttcatgcg atttttttga tacatagtag cttgtagcca
    18841 tctccaagtt tgcagcataa tcactttcag tcgaataaca gatcgtatcc tccccgattt
    18901 ctgagatcgc catgaattct tttgagtctt tcccgcccat tgcgccaccg tctccgataa
    18961 tcgagcgaaa ttctaagcca caacgcttga atacttcggt gtaggctttt tcgtaatctt
    19021 tatatgtctc gtccaaactt tcttccgttg catggaaaga atacgcatct ttcatgataa
    19081 actctcttcc gcgaagcaaa ccgaagcgag gtcgtttttc gtcacgatat ttaggttgga
    19141 tttgataaag atttaaaggt aatttcttat atgaattgat ttcattacgc atcaaatcag
    19201 taaaagtttc ttcgtgagtt ggtccaagga tcatttttcg atcattgcgg tctttgaaac
    19261 gatacaagtt tggtccatat gtttcgtaac gcccggattc ttcccaaagt tctgctggca
    19321 agatggcagg catcagcatt tctaccgctc cgattttttc aaattcttcc cgcataatgc
    19381 gtttcagttt ttccaataca cgatttgcta atggcagata tgaataaata cccgctgata
    19441 cttggcggat atatccagct cttagcaaga tttgatggct taatacttct gcatcatttg
    19501 gaacttcgcg taatgttgga atcaacattt ttgattgtct cattctatat actcctttat
    19561 ttcctcaaaa tagttatcgt ttgttattat accctatttg tcaacttttt ctagaaaaag
    19621 aatcgttgaa tatcgttcca tgtaactagc accatcaaca acattaaaaa tccaaagcca
    19681 gctaatgtca atattccttc tttttcttga cttaatggtt tcccgcgaat cccttcaaaa
    19741 atattcaaga ctaatttccc gccatctaag gcaggaatcg gaagcagatt gacgattccg
    19801 agattcattg aaagaagcgc catcaaacta ataacagttg ttacaccttg atttgctgct
    19861 tctgaagaca attggtacat catgacaggt ccgcctaatt tatctaaact gaaacctgta
    19921 aatagtgatc ctaaggcttt gaaaatttcc aatgaaccgc tgaaagcttg acgcgtccct
    19981 ccaatgatct tatccataaa acctgtattc attggggctt ttataccaag ttggccaact
    20041 ttttcgccat ttgactctac agatttcggt gtcacgtcga cagattgcac ctgaccttct
    20101 cgttcgatct taaagtctaa tgttttatcc ggatttttcg taatgactgt tgtcagatca
    20161 ttccatgaat ggatttcttt tccgtccaca ctgactacct catcattttc ttttaagcct
    20221 gcttctgccg cagctccatt aggcatgatc tctcctacgc gattagtgtt cgtcacttga
    20281 acaccacctt gcataaatgc cagaatgatg aataacagga ttgccaaaat aaagttattc
    20341 atgggtccag caaagttggt cagcatacgc tgccacaatt tagccgattg gaactgtacg
    20401 tcttttggcg caattctgat ttctgttccg tcgttttcaa taatcgtcgc atcgtgatca
    20461 acagggtagg ttactacttc tgtttcgtct ccattcacat aaccagtgat agtcagctca
    20521 tcttctaaat cataacggct caattccatc gggatagcat ttgtcaattg gattttctta
    20581 cttaaattta tcttctctac gatgccgtca gaatttagta aaagagaaag aggcattcct
    20641 ggtgccattt ctgtttcatc atccccattg cctgccattc gaacataacc gccaataggt
    20701 aataggcgaa gcgtatatgt tgtcccgtct tttgcttgat gcccataaat tttcggaccc
    20761 ataccgatag caaattcgcg aacgaggatt cctgatcgtt tcgcaaagaa gaaatgacca
    20821 aactcatgaa caatcactaa tataccaaaa acgatgataa atgtcagaat cgttttcata
    20881 gcttttttct cctttacttg ttttttgtgt tttttataaa acaacgataa aacactataa
    20941 ttttaccata gtataccaaa gacaccaata gtatcaatat aatgaaaaat aaaaacctgc
    21001 gtcttttatg aaattcacaa agaaacgcag gtatacttac taaataatgc caaataaatg
    21061 catgatagga aatacaaaca gcaaactgtc gaaacggtct aagattccac catggcctgg
    21121 taagatattt cccgagtctt tcacatcata atgacgtttg attgcagatt cgaccaaatc
    21181 accaaattgt ccgactgctg aaaaaataat cgtcatcaga agcatgacaa acataccatg
    21241 attaaatagt tcttttgctg ggtatagcaa taaataaaga aatgcgacta caacagcact
    21301 accaatacca ccgagcgcac cttcaatcgt tttgttgggc gaaatatcag gacttaactt
    21361 atgctgacca taacgtcgac caaccatata tgctccgata tcagttgccc aaacaataaa
    21421 tagaccaaat atcaaaacag ccaagctttc tgttcgtgca ttgacaaaat tctggaaacc
    21481 tattcccaca tacaagctga caagcacagg aaaacctgct tcatcaatcg tatacgtatt
    21541 tttagaaata actgatgttc ctaaaagaat cataactata agataaaata acataaatgt
    21601 acttacattc tctggaagaa agaaaaacca tctttctttt ggcaaaacaa gtaacactgc
    21661 accaacggcg gataaaactc cttcaaaact taagagtgtc aatcctttca tccgaaacag
    21721 ctcgtaaact cctattactg ctaagattgc tgctgtaagc tcaaccgcta tgcctccgat
    21781 ccatataatc ggaataaaca agactagcgc cacggctgct gtgaccacac gttgtttcat
    21841 gactgatccc ctttctcatc ttctgttttt tttacaccgc cgaatcgacg gtctcgattt
    21901 tgataagagg cgattgcttc ttcaagatgc gcaccatcaa aatcaggcca aagcgctttt
    21961 gtaaaataca gctcactata tgcgatctgc cataacagaa aattactaat tcgctcttct
    22021 ccacttgtac ggatcatcag ctcaggatct tgtaactctt taggcaagaa acctgtcatt
    22081 aaatgcttag caatcaattc atcatcgatt tcttctgcgt gtatttctcc ttttgctact
    22141 tcttcggcaa tttcttggac agcagtcacg atctctgctc tgcttccata attcaaagca
    22201 aaattcaata ccattccagt attgtttttt gtttgctcaa ttgcacgtct tacagcatct
    22261 tgcgtatgtt ctggcaatac gttttcataa cccatgacat gaactttgac attttcttta
    22321 atcaattctg gtacgaacgt atcaaagaag tcgacaggta gttgcatgag aaaacttact
    22381 tcatcttttg gtcgcttcca gttttcagtc gaaaaggcat aaagcgtcaa cactttcact
    22441 ccaagccttg atgctttttt tgtgactttc tttactgttt ccatgccttc tttatggcca
    22501 gcaactcgtg gcaaacgacg gttttgcgcc caacgaccat tgccgtccat gatgatggca
    22561 atgtgctgag gaattggtcc atcggtatta aattgaaatt cgcttttttc ttgaatatat
    22621 ttgttttttt gcggaaaaaa acgtaacatt ctttttcctc ctatacttaa aagattccca
    22681 ctatcttaag tattatagca atatctaact gtttttccta tgaaatcatt gtttttttat
    22741 ttaaaaactg aataattttt tcttttttta atttttaaat aaaagaaata taaataaaaa
    22801 cagaggaagc ggaatgatcc gcccctctgt ttttatgatt ttattaaact tccaataatt
    22861 ctttttcttt atcagcagcg atcttatcta cttctttgat gctgttatct gtcaatgttt
    22921 gtacgtcttt ttctaatccg cgcaaatcat cttcggtaat atcaccattt ttttgttgtt
    22981 ttttatattc atcgatcgca tcacgacgga tgttacgaat cgcgattttt gcattttctg
    23041 cttctttttt aacatcttta gctaattctt ttctgcgttc ttctgtcagt tgcgggataa
    23101 ctaagcgaat gacatttcca tcattggttg gactgattcc gatatcactt gccataatag
    23161 ctttttcaac atcttgaaga atgctcttgt caaaaggtgt gatcattaaa acacgtgctt
    23221 ctggaatttg gattgatgcc atttggttca atggtgttgg cgcaccgtag tagttcacag
    23281 taatacggtc caaaaggcta gcgttcgcac ggcctgcgcg gatttgccca agttcacgtt
    23341 gaagattttg tgctgctttt ttcatttttt cttttgcttc tgtcataatt gcatctgcca
    23401 tttttatttc ccccttacag ttgttccgat attttcgcct aagattgcac ggcgaatatt
    23461 tccgtgttca tttaaattga agacaagcag tggaatatca ttatccatgc ttagtgaact
    23521 tgcagttgaa tccatgactt gtaatccttt ggcaataact tccagatgag tcagttcttc
    23581 aaattttact gcatttgcat ctactttcgg atcagcagaa taaacaccgt ctacattgtt
    23641 tttagccatc aggataacat ctgcgccgat ttctgccgcg cgtaatgctg ctgttgtgtc
    23701 tgtcgagaag tacgggttgc cggtaccgcc tgcaaagata acgatccgtc ctttttctaa
    23761 atgacgttct gctttacggc gaatatatgg ctctgcgatt tgacgcattt caatcgaagt
    23821 ttgcacgcgt gtcggtacac ccacgttttc caacgtatcc tgtaaagcta aggcattcat
    23881 aactgtagct aacataccca tgtaatcagc ttgagcacgt tccatgccca tttgtgctcc
    23941 gatttgtccg cgccagatat ttccgccgcc cacaacgatt gccatttcaa cgcccagctc
    24001 gtgaacttct ttgatttctt ggatgatttc tttgattact ggtggtttga ttccaaatcc
    24061 tgcgtcccca gctaaagctt ctccactcaa ttttaagact acacgctgat atttcggtgt
    24121 gaccatgttc attcctccaa tattttctat tgccatttta ccatacttag atgatttcga
    24181 cactctaaga ctggtttttc ttgttttgct taaaaaaagg gagcgcacaa accgtgcgtt
    24241 cccgtctgtt attcaacaga attatttttt cacttggctc ataacttctt ctacaaagtt
    24301 atcttcgcgt ttttcgatac cttcgccaac ttcgaaacgt acgaatgttt taacagttgc
    24361 gccttttgat gcaacgtatt tttcaacagt catatcagga tctttaacga atggttggtc
    24421 caccaaagcg atttctgctt tgaatttctt caagcgtcct tctaccattt tttcaacgat
    24481 attcgctggt ttgccttcgt tcaatgcttg ttctgtaaga acagtttttt catgttctaa
    24541 ttcagcttct ggaatttgtg attcgtttac ataacgagga ttgatagctg ctacgtgcat
    24601 agctacgtct cttgcaactg tttcgtcagt tgttccatct agaacagtca atacggcaat
    24661 acgtccgccc atatgcaagt atccaccaaa tgcagcattg tcgtcttttt ctacaacttc
    24721 aaagcgacgg aagctgattt tttctccgat aacttgagtt gcttcgatca agtcagattc
    24781 aattgtacct ttatctgttt tgattttcat tgcagcttcc atgtctgctg gtttgttttc
    24841 agcaacaagt tctgcaattt cttttactaa atcttggaac atttcgtttt tagaaacaaa
    24901 gtctgtttct gagtttactt caacgattgc tgcagtgtta cctttcacag caactgaagc
    24961 caatccttca gctgcaatgc ggtcattttt cttcgcagcc tttgccattc ctttttcacg
    25021 taaaagatca actgcttttt ccatgtcgcc ttctacttca actaatgctt ttttcgcatc
    25081 catcattcct acgccagtca tttcgcgtag ttcttttacc attttagctg taacgtctgc
    25141 catgtttaac tcctcctgtg tgtttttctt tttaaaaaaa gctgcttcaa aggagagtga
    25201 ggcttgtcgc ccagcctttg aaacagctcc atcattcaaa attattctgc tgaagaattg
    25261 tcgccttcaa caacatctac gatttcttcg attgatgttg ctgtgttttc ttctgtgaaa
    25321 tcttcttcaa ctacttgatc ttcaccttgg tttccttcga tgaaagcatc agccattttt
    25381 gaagtaatca atttaacggc acgaattgca tcgtcgtttg aagggattac tacgtcgatt
    25441 tcatctggat cgcagtttgt atcaaccata gctacgattg ggatatttaa tttgtgtgct
    25501 tcttgaacag caatacgttc tttacgtggg tcaacgatgt acattacatc tgggattctt
    25561 ggcatatcag cgataccgcc taagaatttt tcaagacgtt cacgttcttt gttcaaacca
    25621 gcaacttctt ttttaggtag aacttcaaaa gttccatctt cttccattgc attgatttgt
    25681 ttcaaacggc tgatacgttt ttggatagta tcccagtttg tcaatgttcc gcctaaccaa
    25741 cgatggttaa cgaagtattg tcctgcacga gttgcttctt ctttgattgc ttcttgtgct
    25801 tgtttttttg tacctacgaa taatgctacg ccgccttctt cagcaacgct cttcatgtaa
    25861 tcataagctg catctactaa tttaactgtt ttttgcaagt caatgatgta gattccgttt
    25921 ctttctgtga agatatattt cttcattttt ggattccagc gacgagtttg gtgaccaaag
    25981 tgtacgccgg cttctagtaa ttgtttcata gaaattactg ccatgtttgt ttcctccgat
    26041 ttggttttat ttttcctctt cttatcttct accttgccgg caaacttttt caagcaccta
    26101 ccgacaatcc aataagaatg tggatttgct gatgcataaa gcaccctcag ctattataca
    26161 gacaaaagat ccgtatcgca agagcaaata tacttttctg ctcaacattc aatagtttac
    26221 ctgaaagata aaaagaattc aagaaaaaca gtagatttct ctttgtttct ttattatcct
    26281 ttttccataa aataatttta ggtataaaag acgaatatta ttgggcaatc cttctttttt
    26341 ttgttatgat acatgtgaaa tctttcgtaa aggagaaaga aaatgcgcaa agctcaaaga
    26401 catttgctga ttaaacaagt cattgaagaa ttcccgatcc gtacgcaaga agaactatta
    26461 aacaaacttg ggcattatgg cgttaccgcc acgcaggcaa cgatttctcg agatatccgt
    26521 gatctaaaaa tcgtgaaggc acctgatgaa aatggcgttt cccgcttcgt attgtttcgc
    26581 gaaaaaggaa ctcctgagac taggactgag gaagaggatc ggctggttca aatgattgaa
    26641 gacattgtat tgaaagtcga acgagtccac tttttgacga tcgtcaatac cttgccagac
    26701 aatgctcatt tatttgcatc tgttttagat gagatcaaac ctccctctat tgtcagtaca
    26761 attgctggat tcgatacgat catggtcatc tcggaaaatg aggaggacgc aaaacgagta
    26821 gaagaatttc ttcaccatcc aactgaagta tcactttttt aatcagagca cgtattccta
    26881 atatggaatg cgtgtttttt tattttcaat attttcctta ttgaataaac ctttacactc
    26941 tttacaaaaa tacatcattt tttattcaaa tttttcaaca ccttgattga atcacaataa
    27001 tcatcattta tattggatgt taacaagagc agcttcttgc tctttgtgta tttcaaaaaa
    27061 gaaatgaaag gaaatcatat gtataaatta ttaacttctg gaccattgac gacttctgaa
    27121 acagtgaaaa atgctatgct gatcgatcat tgtacttggg atcaagaata caaagaaatt
    27181 acacaatgga taagaaaaaa attattggat cttgctcaag tatcggaagg aatatacaca
    27241 acggtcttaa tgcaaggtag cagctccttc ggggtagaat cggtattaag ttctgtgatc
    27301 aggccagaag atactgtact gatttgttca aacggcgcat atagacaacg aatgatcaac
    27361 atgtgcgaat gtctgaaact gaactatacc gtttatgagg tatcggaaca tcaaatcctc
    27421 caagctcatg tgattacaca gctgattgaa gagaatccgt ctatctccgc actagcaatg
    27481 gtccatagcg agactacttc cggtattttg aataatttgg aagagattgc tgctgtcaca
    27541 caagaaagag atcttttgtt tatagtggac gcgatgtcaa gctttggtgg gataccgatt
    27601 tgtgtagccg aactagggat tgattttttg atttccagct caaacaaatg tattcaaggt
    27661 gttccaggct tttcttttgt acttgctaat aaagcggaac tggaaaaacg aaaaaatgtt
    27721 agccgaagtt tgtcacttga tctatatgac cagtaccgcg tgatggaaaa cgagaatggc
    27781 aaatggcgtt tcacttcccc tacccatacg gttttggctt ttcaacaagc gctgaaggaa
    27841 ctagaggcag aaggaggaat cgaaactcga tattcacgtt atctcgccaa taatcatgtg
    27901 ctacgacaaa aaatgacaga gatggggttc cagcctttca ttcaagaata tcaggggcca
    27961 ttcatcacct cttttcttta ccctaaggac tggcgatttg attttaatca tttttatcag
    28021 tatttaaagc aaaatggatt tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt
    28081 cgtatcggaa atattggcga gatctatccg gaagatattt tggaactgac agcaattatc
    28141 aaagactata aggagaacgt taatgattaa aactgtaatt tttgattggg caggaacaac
    28201 tgtggacttt ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg
    28261 aatccaactg actgacaaag aaatccgcta accgatggga aaattaaaat gggatcatat
    28321 ccaacagctt ctttccctcc cttctgtcaa aaaacaatgg atagctattt atggacaact
    28381 acctcaggaa aaagaggtgg aggaattgta tcaacaattt gaacattatc tgtttaaaga
    28441 tttggtcaag cattctacct tgaaacccga cacattggaa acgatcgaac ggttgagaaa
    28501 cgctgggatc aatattggtt ccacgaatgg atttaatgct cctatgatga caatcgtagc
    28561 agaaaccgca aaaaaagaaa ggtattcacc agacttcatg gcaacttttg aagacgtcga
    28621 agggtatggt cgaccgtttc cttatatgat ccataaaaac atgcagtact tcaaaaacaa
    28681 gtcggttgat gaagtaatca aagtcggaga tacggtctca gatatccagg aagggaaaaa
    28741 tgccggagtg ttgacagtag gtgtaattga aggtagttcg ctaatgggga tttcttatga
    28801 tgagtaccaa aaattagaga tggggaaaaa gatcgtttaa aacagtctgt tgctcttaga
    28861 ttccaacaag ccggcgctga tatttgtgta aacaatttaa aagagttaac agaatacatt
    28921 ttgtacgctg acatgaatcc agaatgactt ttatacaaaa taagaggttg tgaaaaagct
    28981 gtcctgtatc aagtaataaa aacagccaag caaaaatagc tcctcatatt tttcgtttgg
    29041 ttaggcttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaaaggga
    29101 gctgtgacac gacttttgtc acagtcccct tatttgcaaa caaacagtgt ttaagagaca
    29161 acctctccac taaacggtgt tcattcagct gactctcggt atttgctcaa aaaacgataa
    29221 aatttgaaaa gctattttct cttttgtttc tctaatactc ggatcagaac gctgatttca
    29281 cattctcttt ttcttacatc ttatctggtg catgaagtcc tagcaaacga aggtcttctt
    29341 tcaataatac agtcactgca taaactagag ctaagcgtgc ttctttttgt tcatcatcag
    29401 ctaaaatctt cgtatgagca tagtatttgt tgaatgcttg tgctaatttg attgcatgtt
    29461 tcgcaatcac tgacggttcg tatttttccc cagcagacaa gaccgtttca gggtattttt
    29521 gaactaattt aaccacttcc cagctggtat cgtcattcaa tgcatagtct gctgtttctg
    29581 aagggacaaa tcctgctttt tccaacaggc tgactgcacg agcatgtgta tattgcacat
    29641 aaggtccagt ttctccttca aatcgaacaa cttcttccag gttaaagtca aatgtattca
    29701 gtcgatcgtt tttcaaatcg tggaaaatga ctgccccaac cccaacttgt ttagctacag
    29761 catctttgtt ttccaaatca gggtttttct cgctgatttg ttctttagca gattggattg
    29821 cttcatttaa tacttcttct aataagacga tctttccttt acgagttgat aattttttac
    29881 caccttgcgt gatcaaacca aatggaatat ggtgcatgtc gtcagaccag tcaaagccca
    29941 tttccttcaa gacagctttc aactgtttaa aatgatagct ttgttcattc cctactacat
    30001 atagagattg tttgaagtcg taagtacgtt tacggtacaa tgcagcagct aagtcacgtg
    30061 taatgtatag tgtcgcaccg tctgacttct taatcaacgc tggattcagg tcataagcag
    30121 ataggtcaac gatctctgca cctttatcct cgttcagcaa atgtttttct tctaaaagtt
    30181 tgacgatttc atccatcttg tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta
    30241 cttccagcag atcatagatt ttattgaatt ctttcatcga ttcatcacgg aaccattgcc
    30301 ataattgaat agcctcttta tcgccttctt ccagacgttt gaaccaagca cgagcttctt
    30361 catttaattc tggttctgtt tcagctactt catggaattg gacgtataaa cgaagcaatt
    30421 cattgattgg ttctgcttta acagcttctt ctgtacccca ttttttgtaa gcgacgatca
    30481 gttttccaaa ctgtgttccc cagtcaccta aatggttgat gcggattggc tgataaccga
    30541 ttttttccat aatgaaaccg atcgaattac cgataactgt cgaacgcaaa tgtcccattg
    30601 agattggttt ggcaatatta ggagaagaca tgtcgattgg tactgttcct tgattcccga
    30661 tgttgctatc cccataatgt tctttttctt tcactactgt ttgcaaaact tttttgctga
    30721 taagttcttt attcatgaaa aagttcaagt aaggtccgac aacttcgatt ttttcaaagt
    30781 tggcagaatc gattttttct gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa
    30841 cttttgctaa tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat tttggatttt
    30901 ctagtaattg ctcgacttgt tccagtgtta aatcatcttt gacagcgtca tagacagctt
    30961 ttgcgacaag atctttatta ttcatcttta ttcctcctaa agtgttccct tgattgttaa
    31021 acaaaaaaaa tctctagcga cggcatactt agtatgccat cactagagac gagatttccc
    31081 gcggtaccac tccaattgtc ctaaaaaagg accagctcca tctgatatcg gtagacgacc
    31141 gtctgtttaa aacagaattc tccaagtgcg cttcgttgta tcttcttcat tcggcttcca
    31201 ccacttccga attcgctagc caagaatgat acaagtactc tcttgttcaa caaatcttag
    31261 tcttattaaa ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt cgtttcataa
    31321 aaaaacaatc cagatggcct aaaggagaag aaataccacc tggatagcta taaatcattt
    31381 cgtaaatacg aaagacttaa caaattaatc agctacgata atcgttcctt cttcagaagc
    31441 taaaagattc tcgatatttt ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc
    31501 aacaaatgca atagctgctt ctactttcgg aagcatgctt cctggagcga attgcttttc
    31561 atcaatgtat tgtttcattt ctgaaacagt aacagtttct aatttctttt gatctggttt
    31621 ttggtaattg acatacacat tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat
    31681 gatttcggct aatttttctg aagcaaaatc tttatcgatt acggcttctc gtccttctaa
    31741 tccagtagct gtttctacaa caggaatccc accgccacca acagagactg tgataactcc
    31801 ttgatcgacc agcgtttcga tgacacgtgc ttctttaatc gagatgggtt taggggaagc
    31861 gactactttt ctccatccac gtccagcatc ttctttgaat gtcacagtgg agtcagcatt
    31921 catttgttct ttggcttctt cttccgtgta gaatggaccg actggtttac ttggattttt
    31981 gaatgaagga tcattttcat ccacaataac ttgagtgact aaagaaacca catctttatc
    32041 gatccctttt tccttcagta cttcgcccat ggcgttttgt aaccagtaac cgatagatcc
    32101 ttctgtcatt gcaacacatg tatctaaagg cattgccggt gttttctcag aatcagcagc
    32161 ttgctgctga atcaataggt taccgacttg cggaccattt ccgtgggaga tgatcaattc
    32221 atctccttgt tcgataaatt tcactaaata ctttgctgtt tccattaacg cttcttgttg
    32281 ggcttttgca cttgcatccg tagacaagat ggcattgccc cctaaagcga caactacttt
    32341 tcgtttggcc atttcattcg tcctttcttc agttctaccg aattttctag ataacaaaac
    32401 gagccagcgt tttgtgattt tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg
    32461 ttatgcttaa acgcgaggga taaacaagtt acctaatgtt gctgccatga tcgctttgat
    32521 tgagtgcata cgattttctg cttggtcaaa ttgacgagca tatttgctgc ggaagacttc
    32581 atctgttact tccatttcag tgatgccaaa gcgttctttc atttgttcac cgtaaacagt
    32641 gtttgtatca tggaaagctg gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt
    32701 tttttctacc atttccatat tgatttggta aggtttcaac aatttgatac gttcttcgaa
    32761 tttatcttct tcacccattg atacccatac atcagaataa aggacgtctg ctccgtctac
    32821 acctttatca acatcgtcgg taatcatcaa ttgtgcaccg gatttttcag caaagccttc
    32881 tgccattttg acgatttctt cttcaggttg taattctttt ggtgcaacga tacgcatatt
    32941 cgctcctaaa attgcacctg tgactaataa agagttagcc atattgtttc gaccatctcc
    33001 gcagtaagca acagtgatac cttctactgt tccaaagttt tcttggatag ttaaaaagtc
    33061 agcgatcatt tgtgttggat gccattcatc agtcaagcca ttccaaactg gaacaccaga
    33121 gaattctgct agttcctcta ccattttttg actgaatcca cggaattcga ttccgtcaaa
    33181 catacgtcct agtactttag ctgtatcttc tgtggattct tttttaccta attggatatc
    33241 atttgcacct agatattcag gatgtgcacc tagatcgatc gctgcagtag taaatgcaga
    33301 acgagtacgt gtagatgttt tttcaaataa aagcgcaata ttttttcctt ctagatagtg
    33361 atgtgggata ccacgttttt tcaagtcttt caagtgttct gaaaaatcga tcaaatattg
    33421 taattcttct tttgtaaagt ctttttctgc taataagctt cttccttgga atactgattc
    33481 tttcatatta aacattctcc tttagattgt ttttatttaa gattaaagag gaaatcccga
    33541 cggcagctga cagtaagact gccgtcagga aacaaattac ttcaagtctt cacggacaag
    33601 tggttggctc atgcagcgtg gaccaccacg acctcgtgac aattcacttg aattgatttc
    33661 taacactttg atgccatagc tacgaagcaa ttcgttcgat acataatttc ggttataagt
    33721 gactacaaca cctggagcga ttgccaatgt gtttgatcca tcattccatt gttctcgagg
    33781 tgcaacgatc tcatctccat taccagttgg gatcagtaca agatcatcta atccaagagc
    33841 agctttcatc gtttcgtgta agttatctga atgagtaatc ttgatatcat caccatctgg
    33901 ttcgattgta aagacatcca tcttaccgtc tttactttga attgctggat ggatagtgaa
    33961 tttatcgtga tctaccattg taaatacggt atccaaatgc atcattgcac ggttgttcgg
    34021 aattttgatt gccaacactt tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa
    34081 tttttctaaa gctttcgcat tagttcgttg agaaatccca acagctacta ctttatcgct
    34141 taagatcaat tcgtctccgc cttcgatatg atctggattt tcacgatcca accatacttc
    34201 gattccttta tttgcaaatc ttggatgatg tttcaaaata tattctgtaa acatcgattc
    34261 acgacgacgt gcttcaaaag tcatcttatt gactgtcatc ccatttccca ttgatgcgga
    34321 agggtcgcgt gtaaagtaca agtttggcat tgggtccatg taaaatggat agtcatcgtc
    34381 tgctgacaga tcatatagac tgctagaacg tacttcgata tcttttgtac gaaccccagc
    34441 catgatttta tctaccattt cttgcgtttc cattgataga aggaactcat gaagaccctc
    34501 tcttacagca ttcgatgcaa tatgagattc atccaacatt ttattcaaga attgttcttt
    34561 gacattgcct gcgtcaatcg cttctgccgc taatttttcc aagtaaagag tctcgacgcc
    34621 ttcattttgc aatgtcttcg caaagttgtc atgttcttct tgagcaatcg gaagatatgg
    34681 aatatcatcg aacaacagac gatccataat atctggtgtc aaattttcca cttcttgtcc
    34741 aggccgtttc agtaaaactg tctttaattt ccctatttca gagaaaacgt gaataggttt
    34801 atccatgttg tgttgcctcc ttctttttga ttacatgtat aggatatcct ccttttgtaa
    34861 tcactttcat aattttgtct cattcaaaat gagacattcc tcacattaat attcgataat
    34921 taaataatta aacgtttttc tatttttaag cgtaatttat tcatttttta ttctgtatac
    34981 actttcaaaa aaatatttat tcattgaaaa aatttttttg aataaattaa aaaaata
//
LOCUS       DAZUMB010000015        32612 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000015 DAZUMB010000000
VERSION     DAZUMB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.015"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1309
                     /locus_tag="ACG80L_000618"
     CDS             164..1309
                     /locus_tag="ACG80L_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5022900.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="ACG80L_000619"
     CDS             1446..1790
                     /locus_tag="ACG80L_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS5022901.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="ACG80L_000620"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="ACG80L_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS5022902.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="ACG80L_000621"
     CDS             complement(3485..4084)
                     /locus_tag="ACG80L_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5022903.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="ACG80L_000622"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="ACG80L_000622"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS5022904.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="ACG80L_000623"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="ACG80L_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS5022905.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="ACG80L_000624"
     CDS             7290..8408
                     /locus_tag="ACG80L_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS5022906.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="ACG80L_000625"
     CDS             complement(8468..9760)
                     /locus_tag="ACG80L_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5022907.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="ACG80L_000626"
     CDS             9910..10470
                     /locus_tag="ACG80L_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5022908.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="ACG80L_000627"
     CDS             10599..10976
                     /locus_tag="ACG80L_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS5022909.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="ACG80L_000628"
     CDS             10909..12981
                     /locus_tag="ACG80L_000628"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5022910.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="ACG80L_000629"
     CDS             complement(13192..14139)
                     /locus_tag="ACG80L_000629"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5022911.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="ACG80L_000630"
     CDS             14385..14849
                     /locus_tag="ACG80L_000630"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS5022912.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="ACG80L_000631"
     CDS             complement(14911..15954)
                     /locus_tag="ACG80L_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS5022913.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="ACG80L_000632"
     CDS             complement(16331..17296)
                     /locus_tag="ACG80L_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS5022914.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="ACG80L_000633"
     CDS             17530..18375
                     /locus_tag="ACG80L_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5022915.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="ACG80L_000634"
     CDS             18566..19753
                     /locus_tag="ACG80L_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5022916.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="ACG80L_000635"
     CDS             19759..20115
                     /locus_tag="ACG80L_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS5022917.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="ACG80L_000636"
     CDS             20117..20455
                     /locus_tag="ACG80L_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS5022918.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    20531..20787
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20850..21884
                     /locus_tag="ACG80L_000637"
     CDS             20850..21884
                     /locus_tag="ACG80L_000637"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5022919.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="ACG80L_000638"
     CDS             21877..22563
                     /locus_tag="ACG80L_000638"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5022920.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="ACG80L_000639"
     CDS             22587..23435
                     /locus_tag="ACG80L_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5022921.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="ACG80L_000640"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="ACG80L_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS5022922.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="ACG80L_000641"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="ACG80L_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS5022923.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="ACG80L_000642"
     CDS             25701..26936
                     /locus_tag="ACG80L_000642"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS5022924.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /gene="sufU"
                     /locus_tag="ACG80L_000643"
     CDS             26923..27393
                     /gene="sufU"
                     /locus_tag="ACG80L_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS5022925.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="ACG80L_000644"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="ACG80L_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS5022926.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="ACG80L_000645"
     CDS             complement(28884..30026)
                     /locus_tag="ACG80L_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5022927.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..30799)
                     /locus_tag="ACG80L_000646"
     CDS             complement(30065..30799)
                     /locus_tag="ACG80L_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5022928.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31031..31306
                     /locus_tag="ACG80L_000647"
     CDS             31031..31306
                     /locus_tag="ACG80L_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5022929.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            31404..>32612
                     /locus_tag="ACG80L_000648"
     CDS             31404..>32612
                     /locus_tag="ACG80L_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022930.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSG"
BASE COUNT        10669 a         5886 c         6858 g         9199 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    30181 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    30241 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    30301 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    30361 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    30421 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    30481 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    30541 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    30601 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    30661 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    30721 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    30781 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    30841 gtgttgcaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    30901 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    30961 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    31021 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    31081 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    31141 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    31201 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    31261 taaaaaatct gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga
    31321 ctaaaacata tagtaataaa gagtttgttt ttattatctg tatgttttag tctttaacag
    31381 aattttataa ggaggaaaca caaatggaaa catcaatgat taatattcca atttatgtag
    31441 atcaagaaaa attattagag aagccagtta caaaaaaaga aaaaactgat gtaagagata
    31501 ggtgtttaga taactataga gaagttacaa gagatgagtt tgtagagata ctaaattcat
    31561 cacaatcatt tattccaagc aagacaaaga ataaaggcaa tgacaaggag agttttgtgg
    31621 aaaccagagt aattattctg gatgtcgata acactgttaa agatgagaag gggaaggtag
    31681 tagatttgag taaagatgat agtagatact taagcgtaga aaaggtttta tctatcaatt
    31741 tagttcataa ttctgcgttt gcgatacaaa aaagtattcg gtactctgaa aatctagaaa
    31801 aatacaagat tgtctttcta ttaaaagaag caattactga ctatggtaaa atggttgctg
    31861 tttacgaata cttgaaaaag aacatccccg ggtgtgatga caatgtaaat gcttcgaata
    31921 gaatgttttt tggaggatat aagtcagatg cagtgataat aattaatgaa gacaacatgt
    31981 tagatattac tgagcttccg attgatttta ccatgacatg ttctaaaaat tgtcaaagtg
    32041 attcagggag agagtatgaa ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg
    32101 acgacaaaga agaaatgaaa cagtggttta aagactgcct gtttgattcc tccgacatgt
    32161 cttttaatga gatttatgaa agattgttaa ccattgatat gaataaatta cttaagtcaa
    32221 gtaaaaactt aagatgtctg tttcacaacg atcataatcc ctctgctagt atttttacca
    32281 gtaaaactgg gcattccatt ttttattgtc attccagtaa ttgtgatatc agtgaaaatt
    32341 ttattggagt tgtgatgcta attttaaaga aagacagtcg ggtagaaaca tttaattggc
    32401 tattagagaa cctagacttg tatcctacac attttcgaaa attgaaagag gaatcaaaag
    32461 tactttttga cactcttgaa tcaatgaaag acaaactttt caggacaata aggaattatc
    32521 tagaggaaat gcgacgtgtc tatgatgtct tgctttctga tgttaatttt tttgatgata
    32581 gtaaggaaag tgtgacttgt attttgtcgg gg
//
LOCUS       DAZUMB010000016        32129 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000016 DAZUMB010000000
VERSION     DAZUMB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.016"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACG80L_000649"
     CDS             complement(407..766)
                     /locus_tag="ACG80L_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS5022931.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACG80L_000650"
     CDS             complement(796..1137)
                     /locus_tag="ACG80L_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022932.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACG80L_000651"
     CDS             complement(1134..1808)
                     /locus_tag="ACG80L_000651"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5022933.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG80L_000652"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG80L_000652"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS5022934.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACG80L_000653"
     CDS             complement(4322..5512)
                     /locus_tag="ACG80L_000653"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5022935.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACG80L_000654"
     CDS             complement(5533..6060)
                     /locus_tag="ACG80L_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS5022936.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACG80L_000655"
     CDS             complement(6107..8896)
                     /locus_tag="ACG80L_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS5022937.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACG80L_000656"
     CDS             complement(9043..9243)
                     /locus_tag="ACG80L_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5022938.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACG80L_000657"
     CDS             complement(9696..10016)
                     /locus_tag="ACG80L_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022939.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10061..10231)
                     /locus_tag="ACG80L_000658"
     CDS             complement(10061..10231)
                     /locus_tag="ACG80L_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022940.1"
                     /translation="MTKIISQIFVKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKN
                     IIYEQNVNNGRS"
     gene            complement(10519..11637)
                     /locus_tag="ACG80L_000659"
     CDS             complement(10519..11637)
                     /locus_tag="ACG80L_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022941.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACG80L_000660"
     CDS             11870..11992
                     /locus_tag="ACG80L_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022942.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACG80L_000661"
     CDS             12108..13415
                     /locus_tag="ACG80L_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS5022943.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACG80L_000662"
     CDS             13536..14735
                     /locus_tag="ACG80L_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5022944.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACG80L_000663"
     CDS             complement(14762..16030)
                     /locus_tag="ACG80L_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS5022945.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACG80L_000664"
     CDS             complement(16200..16841)
                     /locus_tag="ACG80L_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS5022946.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACG80L_000665"
     CDS             complement(17250..17753)
                     /locus_tag="ACG80L_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5022947.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACG80L_000666"
     CDS             complement(17876..18640)
                     /locus_tag="ACG80L_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS5022948.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACG80L_000667"
     CDS             18748..19023
                     /locus_tag="ACG80L_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS5022949.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ACG80L_000668"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ACG80L_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS5022950.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ACG80L_000669"
     CDS             complement(20579..20980)
                     /locus_tag="ACG80L_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5022951.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ACG80L_000670"
     CDS             complement(20994..21803)
                     /locus_tag="ACG80L_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5022952.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ACG80L_000671"
     CDS             complement(21790..22680)
                     /locus_tag="ACG80L_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5022953.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ACG80L_000672"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="ACG80L_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="ACG80L_000673"
     CDS             complement(23348..24289)
                     /locus_tag="ACG80L_000673"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFS5022954.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACG80L_000674"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACG80L_000674"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5022955.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ACG80L_000675"
     CDS             complement(25274..26470)
                     /locus_tag="ACG80L_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5022956.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ACG80L_000676"
     CDS             complement(26488..27216)
                     /locus_tag="ACG80L_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5022957.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACG80L_000677"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACG80L_000677"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5022958.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ACG80L_000678"
     CDS             complement(28628..29086)
                     /locus_tag="ACG80L_000678"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5022959.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ACG80L_000679"
     CDS             complement(29083..30546)
                     /locus_tag="ACG80L_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5022960.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACG80L_000680"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACG80L_000680"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5022961.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32129
                     /locus_tag="ACG80L_000681"
     CDS             31602..>32129
                     /locus_tag="ACG80L_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5022962.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTN"
BASE COUNT         9364 a         6916 c         5509 g        10340 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgtt ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaat
//
LOCUS       DAZUMB010000017        31934 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000017 DAZUMB010000000
VERSION     DAZUMB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.017"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..693
                     /locus_tag="ACG80L_000682"
     CDS             286..693
                     /locus_tag="ACG80L_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022963.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="ACG80L_000683"
     CDS             707..1108
                     /locus_tag="ACG80L_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022964.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="ACG80L_000684"
     CDS             1110..1481
                     /locus_tag="ACG80L_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022965.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1517..1819)
                     /locus_tag="ACG80L_000685"
     CDS             complement(1517..1819)
                     /locus_tag="ACG80L_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5022966.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2077..2190)
                     /locus_tag="ACG80L_000686"
                     /pseudo
     CDS             complement(2077..2190)
                     /locus_tag="ACG80L_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2573..3805)
                     /locus_tag="ACG80L_000687"
     CDS             complement(2573..3805)
                     /locus_tag="ACG80L_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS5022967.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4059..4628
                     /locus_tag="ACG80L_000688"
     CDS             4059..4628
                     /locus_tag="ACG80L_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5022968.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4806..5246)
                     /locus_tag="ACG80L_000689"
     CDS             complement(4806..5246)
                     /locus_tag="ACG80L_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS5022969.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5404..6168
                     /gene="map"
                     /locus_tag="ACG80L_000690"
     CDS             5404..6168
                     /gene="map"
                     /locus_tag="ACG80L_000690"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS5022970.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6200..7123
                     /locus_tag="ACG80L_000691"
     CDS             6200..7123
                     /locus_tag="ACG80L_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS5022971.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7199..8338
                     /locus_tag="ACG80L_000692"
     CDS             7199..8338
                     /locus_tag="ACG80L_000692"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS5022972.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8331..9131
                     /locus_tag="ACG80L_000693"
     CDS             8331..9131
                     /locus_tag="ACG80L_000693"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5022973.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9131..9958
                     /locus_tag="ACG80L_000694"
     CDS             9131..9958
                     /locus_tag="ACG80L_000694"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5022974.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9963..10670
                     /locus_tag="ACG80L_000695"
     CDS             9963..10670
                     /locus_tag="ACG80L_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5022975.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            10770..11636
                     /gene="rfbA"
                     /locus_tag="ACG80L_000696"
     CDS             10770..11636
                     /gene="rfbA"
                     /locus_tag="ACG80L_000696"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS5022976.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11650..12222
                     /gene="rfbC"
                     /locus_tag="ACG80L_000697"
     CDS             11650..12222
                     /gene="rfbC"
                     /locus_tag="ACG80L_000697"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS5022977.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12244..13272
                     /gene="rfbB"
                     /locus_tag="ACG80L_000698"
     CDS             12244..13272
                     /gene="rfbB"
                     /locus_tag="ACG80L_000698"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS5022978.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13370..14221
                     /gene="rfbD"
                     /locus_tag="ACG80L_000699"
     CDS             13370..14221
                     /gene="rfbD"
                     /locus_tag="ACG80L_000699"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS5022979.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14256..16289
                     /locus_tag="ACG80L_000700"
     CDS             14256..16289
                     /locus_tag="ACG80L_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5022980.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16333..17613
                     /locus_tag="ACG80L_000701"
     CDS             16333..17613
                     /locus_tag="ACG80L_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS5022981.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17823..18629
                     /locus_tag="ACG80L_000702"
     CDS             17823..18629
                     /locus_tag="ACG80L_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5022982.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18641..19861
                     /locus_tag="ACG80L_000703"
     CDS             18641..19861
                     /locus_tag="ACG80L_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5022983.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSNGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19851..21437
                     /locus_tag="ACG80L_000704"
     CDS             19851..21437
                     /locus_tag="ACG80L_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5022984.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21476..23614
                     /locus_tag="ACG80L_000705"
     CDS             21476..23614
                     /locus_tag="ACG80L_000705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5022985.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(23982..24965)
                     /locus_tag="ACG80L_000706"
     CDS             complement(23982..24965)
                     /locus_tag="ACG80L_000706"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5022986.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            25343..26734
                     /locus_tag="ACG80L_000707"
     CDS             25343..26734
                     /locus_tag="ACG80L_000707"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5022987.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            26750..27790
                     /locus_tag="ACG80L_000708"
     CDS             26750..27790
                     /locus_tag="ACG80L_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5022988.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            27809..28894
                     /locus_tag="ACG80L_000709"
     CDS             27809..28894
                     /locus_tag="ACG80L_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS5022989.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            28927..29889
                     /locus_tag="ACG80L_000710"
     CDS             28927..29889
                     /locus_tag="ACG80L_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFS5022990.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            29882..31309
                     /locus_tag="ACG80L_000711"
     CDS             29882..31309
                     /locus_tag="ACG80L_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS5022991.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            31311..>31934
                     /locus_tag="ACG80L_000712"
     CDS             31311..>31934
                     /locus_tag="ACG80L_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFS5022992.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFF"
BASE COUNT        10454 a         5103 c         6282 g        10095 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atgaatttca agcgagtttg
     1501 tttgttattt ttttatttaa tctttgtatc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcacgata tcaaacaaaa
     1621 agtatacacc cttcttcatt ctagttttcg cagcaggaat tttgaaatag ttcttattag
     1681 aatagtagag attgattaat aagctatctt cgattgctaa aaagaaaact tctgaattcc
     1741 atactttata aaaatctttg ataaatctat tcgaagggtc aaatttaaac cataattgtg
     1801 tttttccttc catcagcata aaagttcacc tcaaaaagag tatacgaacg aatgttcttt
     1861 ttgtgaaggc ggaatttaat atactgaata atagaaaaca agtacttgtg ccaaattatg
     1921 tgccaaaaaa attcgaattc aacaaaatgt aaacaaatag aaaaccttct atattaagat
     1981 ttcctgcaat aacaaacaca acagaagacg tgtaatagtt agtcaggaac gtacaaataa
     2041 cccctgtatc ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa
     2101 actgctatgt cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat
     2161 agatgcatgc gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt
     2221 gattactaaa gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat
     2281 tcgaattaaa cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc
     2341 ttaactctag cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg
     2401 actttttatt atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag
     2461 taccatataa aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta
     2521 gatcctttat tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc
     2581 aatcgccatt tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca
     2641 tcttgtcaga tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg
     2701 ctaattcttc atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta
     2761 gtgctaagtg attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt
     2821 gagaaatagg tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat
     2881 ctgtatcaag cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt
     2941 ttccatcttt aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct
     3001 ttgtacttga aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg
     3061 tcggcatatt tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa
     3121 gatggttttt aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag
     3181 aaaattgttc tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc
     3241 atgctaagac agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg
     3301 cctcaacttg tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag
     3361 ccgcagccac gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta
     3421 gtgctttgcc attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg
     3481 catcgggatc ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg
     3541 cttgatcgat tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa
     3601 attctctttg gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat
     3661 aagcttcttg tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa
     3721 ctgtgtgtcc tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta
     3781 gaagttcatt aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac
     3841 tattagcatg ataccattta attatgattc aaacaatgag gctttaaata aaaaaaggag
     3901 gttatttttt taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga
     3961 agaaaatcaa aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg
     4021 gtggaagcgt tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta
     4081 ctagagaaca gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat
     4141 ttactgcgag aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt
     4201 ttaaaaatat ggatgacttg agacaagctc tttttgagca gatctatgat catttacaat
     4261 atgaagtgtt cactgttgag catacaggga atacgattat cgatcttgct tttaattata
     4321 tccattttgc aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg
     4381 gaggaaaaga gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc
     4441 cagcttatgt gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca
     4501 tagctactgg gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga
     4561 ttgaaaagat gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa
     4621 tcgattgaat atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata
     4681 aggaaaagtt cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg
     4741 aatgcaatga ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa
     4801 gtatattaaa tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct
     4861 tcttcagcat tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca
     4921 aaagcagcat cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat
     4981 ccgcagacac cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct
     5041 acgatctcat caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct
     5101 gcctcctcga attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc
     5161 agattttcaa atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta
     5221 gcatatacga tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt
     5281 atgaattata ccaaaacatc attgaaaaga aaataaaacc taaagacacc tggggaatgt
     5341 catttggcta aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa
     5401 aatatgatta ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta
     5461 ttagcagatg tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat
     5521 attgaggtat ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc
     5581 tttgaagggt atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc
     5641 cctcgtaaaa agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg
     5701 aaaggcggca tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc
     5761 gaccgattga tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta
     5821 gggaatcgga ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta
     5881 gcgatcgttc gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca
     5941 gttcctcatt acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg
     6001 atcgaaccta tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact
     6061 gcgtatacaa gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa
     6121 gatggtccaa gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca
     6181 ataggtacgg gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat
     6241 gcgctttatt gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt
     6301 cgtcgttgct tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat
     6361 tttaccgttt ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc
     6421 tgagaccgtt taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg
     6481 aggcttgtta tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc
     6541 tttgcaaatt gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt
     6601 ccgtttcttc tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat
     6661 cgttcttggg ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt
     6721 gaactttatc gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt
     6781 catcatgttc ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat
     6841 tccgggcacg atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta
     6901 tgctaggtat tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact
     6961 catgctttgg cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt
     7021 tgttcaagaa tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg
     7081 gttcaataaa ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt
     7141 gtaaatttat atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat
     7201 gcttgtttct tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt
     7261 aacacctgta ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag
     7321 aagaataaat aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt
     7381 tatatcgact ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt
     7441 gattgcttct ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc
     7501 taaacaaaaa agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat
     7561 acgtattgat tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt
     7621 tcccgtaacg attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt
     7681 ggatgggctg gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata
     7741 ttttttcctc catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag
     7801 tattatcggt ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg
     7861 agctctattc ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac
     7921 ttttgtttca tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt
     7981 tgctatcatt aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct
     8041 tcatcaccga ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc
     8101 gatggctttg atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac
     8161 gatcgtattg attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct
     8221 gatcaatgag aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta
     8281 ccgtacgcaa gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca
     8341 aattaaacca gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga
     8401 cactagataa ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg
     8461 acaacaattc acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca
     8521 tcgatatcta ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga
     8581 tagctagcga ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga
     8641 cagttacagc attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta
     8701 agattcttaa tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg
     8761 attacgtttt gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg
     8821 ctgtgtttga atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag
     8881 gtagttttat ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt
     8941 tcttcatgta ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt
     9001 tattgtacac acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg
     9061 caaaactgtt tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc
     9121 gttttttctg atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag
     9181 tcaatctcca gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat
     9241 tatttacgat aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt
     9301 ttatcatcat gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca
     9361 agcagatgaa ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga
     9421 ttattttgaa ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat
     9481 gattttttct gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac
     9541 agctcaagca gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg
     9601 tgttgcctgg tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt
     9661 agattttttg gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat
     9721 ccttcctgta cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa
     9781 tcgataccgt tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca
     9841 tttatccagt caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa
     9901 aaaccggcat atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat
     9961 tcgtgcgttc ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag
    10021 atagtatttt atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag
    10081 tagatgatac atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt
    10141 tttcggggcc tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag
    10201 gagatctaat ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga
    10261 tcactgacta ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg
    10321 tggacagcga gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag
    10381 gatggcttaa taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt
    10441 ctttaaaacc cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg
    10501 gtttgctggg aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac
    10561 atgatttaaa cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc
    10621 gtgttcatct gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt
    10681 ttaaatcctt aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa
    10741 tttgaaatca aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg
    10801 gaacacgtct gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca
    10861 aaccaatgat ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga
    10921 ttatttcaac gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt
    10981 taggactgca gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta
    11041 tcattggaga agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt
    11101 actatggtgg cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga
    11161 cagtatttgg ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga
    11221 atatgcaagc tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa
    11281 ctggccttta tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag
    11341 aacgtggaga actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat
    11401 ccgtagaact tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc
    11461 ttgaagcatc tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt
    11521 tggaagaaat tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac
    11581 agccattgaa aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag
    11641 tccttacaga tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac
    11701 gtctttggcg atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa
    11761 catggattga aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc
    11821 cttcgggggc ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc
    11881 actggagcag ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa
    11941 tgggacggat ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat
    12001 gcccatggat tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac
    12061 tataatgcag aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg
    12121 ccaattgatc catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa
    12181 gaatttgaag cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt
    12241 cttatgaaaa acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat
    12301 tacgtggtaa acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca
    12361 ggaaacaaag agaacttggc cggacttcct agcgatcgcg tagaattggt cgtaggagat
    12421 gttgcagatg cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac
    12481 gcggcagaat cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat
    12541 atcattggta cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac
    12601 gtatctactg acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg
    12661 gaaggcgaag gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct
    12721 tctacaaaag ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt
    12781 gcaaccattt caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt
    12841 cctcgccaag taacgagtat cttaagcggt attcgtccaa aattatatgg cgaaggaaag
    12901 aatgttcgtg actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg
    12961 aaaggcagaa ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc
    13021 gtgatggaat tgatcttaga aatgatgggc caaccaaaag acgcatacga ccatgtaaac
    13081 gaccgcgcag ggcacgattt gcgttatgca atcgattcga caaaattgcg agaagagcta
    13141 ggttggcagc ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac
    13201 gaagaacatg aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa
    13261 aatggacaat aatacagagg aaatttcact gtattaatgg aaaaacacat gtgaggcagg
    13321 cgctcgcctg tctctttttt aaaataaaaa tcaaggagcg gataaccata tgtttttatt
    13381 aactggagga aatggacaat taggtacaga attgcgccat ttattagatg aaaaaaatct
    13441 gccatatgtt tcaacagatg caaaagactt ggatatcaca gatgctgaga aaacaatggc
    13501 ttatattaca gaattaaaac ccgatgttat tttccattgc gcagcttaca cagcagtaga
    13561 taaagcagaa gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa
    13621 tgtagcactt gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta cagactatgt
    13681 tttcgatgga aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa
    13741 tgaatatggt cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta
    13801 ttatatcata cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat
    13861 gcaacgccta gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc
    13921 aacttggaca cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt
    13981 tggtatttat catttgtcga acgaaaatag ctgttcttgg tatgagttcg caaaagaaat
    14041 attgaaagat acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc
    14101 aacacgtccg cagtattcag tcatgagcct gaaaaagaca gaagcgttag gttttgtcat
    14161 accaacttgg caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta
    14221 atttcctttt tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac
    14281 gacgaaaaga aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt gacctgtttc
    14341 taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa
    14401 acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa
    14461 ctgatgtgat ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac
    14521 ctttttacct ctcacaagct gaagaggact tttcgttggt gaacgaggat tattcattaa
    14581 acggacaaaa catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa
    14641 aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg
    14701 cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga
    14761 ggaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt
    14821 ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg atgattgctt
    14881 tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg
    14941 tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg
    15001 cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat
    15061 gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg
    15121 ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag
    15181 gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa
    15241 attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct
    15301 atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata
    15361 gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct atcttttgga
    15421 ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat
    15481 taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt
    15541 atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg
    15601 catggtctta tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg
    15661 aaaccatcgc agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga
    15721 gacgcttgtt ttacctcgct tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc
    15781 cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa
    15841 tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc
    15901 caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga
    15961 agaaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag
    16021 cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca
    16081 cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat
    16141 taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag
    16201 acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa
    16261 ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta
    16321 gtagaaagga gagtgaagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt
    16381 gtcggtttct tgcttttgtt gctgacgcta gtcttttttg tttggacagc aggggtaggt
    16441 tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct
    16501 attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt
    16561 gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt
    16621 ggagacttac tgtttccttt ggttatttta ttttgtttag taattaaatg gatcgagtgg
    16681 acaaaatggg atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta
    16741 tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat
    16801 tcgctttctg atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct
    16861 ttattgatca gtggatttgc cagttctttt gaacgatggt atgttcgaat catcggtggg
    16921 atagcagtcg ttgcagcctt tttaggtatt tgggtctgcc aatctagagg tgcattagtc
    16981 gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt
    17041 gcgccattta ttgcctacac ggttacgatt cttttagctt tgccgatttc ttatctggcg
    17101 gctgtctctg aaaaagtcaa tttgtttacc gatcgagaag atatttggca taaattctat
    17161 cagacgctag gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccag
    17221 cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga
    17281 ttgataggat ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg
    17341 aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg
    17401 cagtcattta tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg
    17461 ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg
    17521 gaagaggcat atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc
    17581 gctcattctt ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg
    17641 acaaccgtcg cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa
    17701 acgttagaga aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta
    17761 ccaaaaatat ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac
    17821 aaatgtttaa agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc
    17881 tacagttttc gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat
    17941 gggcgtttat caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac
    18001 tgaaagcaca aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga
    18061 ttccctggtt cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata
    18121 cttatttagt aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat
    18181 ccaatctgta cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg
    18241 gtatatatcc ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt
    18301 tcttgacagg attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc
    18361 aattaattac gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta
    18421 atcagtttga ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc
    18481 aaggttatcg tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt
    18541 tgtatttctg ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat
    18601 tgaaaccaca tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag
    18661 actaaaagat atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga
    18721 agccctcaat ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa
    18781 tgtagaaata aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc
    18841 tacaatgttg aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga
    18901 aggaaaaatc tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta
    18961 tgaaaatatc tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat
    19021 ggtcgatgat atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac
    19081 ttattcaaac gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga
    19141 tattttgatt gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat
    19201 ggaaaaattt acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat
    19261 cagttcgatt cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga
    19321 atatggcgat acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt
    19381 taaaacagtg gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga
    19441 cgtgacgaaa gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg
    19501 agaaaaacta tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt
    19561 tttaggaatt gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact
    19621 agatggtatc aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa
    19681 gttaggtttg ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac
    19741 ggttcctttg gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg
    19801 agaaggcatc gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg
    19861 attttgaaat ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac
    19921 caggaagtaa agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg
    19981 gagcaaaaca atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca
    20041 gcgaatttgc tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt
    20101 atgctggaca gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc
    20161 ctggaaaaac attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta
    20221 cattccctaa cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt
    20281 gggcttctta tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc
    20341 aaaaagtttt tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg
    20401 taggtgatac agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt
    20461 tcaaaatgcg accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag
    20521 tagcacaaag ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa
    20581 cccagcaaac gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg
    20641 aaaatgagac actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg
    20701 cgcaacaacc tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta
    20761 tcgattcgaa tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat
    20821 taccagagtt cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc
    20881 gctttatcgg ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg
    20941 ctgaagtcac aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc
    21001 ttcttgagga aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca
    21061 cattgttgga aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa
    21121 cttcgaaaaa aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg
    21181 cagaaacaaa aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct
    21241 tgtcctataa gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag
    21301 aagaagtcaa tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa
    21361 tcctttgcga gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat
    21421 ggatcattga aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca
    21481 aaaaaaagaa attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt
    21541 gcttattgtg ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga
    21601 aaaagaaaat gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata
    21661 taatcttgat gttaagaccc aaagcggttt caaggtacgt ttatcaggga aaatgagagg
    21721 aaaagccatc ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa
    21781 cggtaaatac ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact
    21841 gaaaaaaggg ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa
    21901 attggaattg aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc
    21961 agatttgaaa gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat
    22021 acttatgcct gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact
    22081 ggatcaaacg tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc
    22141 tatccgaaag tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg
    22201 aaaagaaaat ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga
    22261 atttatagca ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc
    22321 aaaagtgcta aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga
    22381 tgcagatggc aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat
    22441 gggaaataat tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg
    22501 cggtttccgt aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga
    22561 acaaatccct gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat
    22621 cccaggatca actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa
    22681 agcattgaca gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat
    22741 ttctggattt tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat
    22801 tccaacgaag aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac
    22861 gtcttattca aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca
    22921 agaattattt gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga
    22981 cattccattc aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata
    23041 ctttttattc ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt
    23101 ttcttatgca cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga
    23161 tacaacgcag catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa
    23221 taactatgat cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt
    23281 agcagtcact gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt
    23341 tgatgaaaca ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact
    23401 gggtcgttac aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag
    23461 aggttatgaa gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca
    23521 agataaatgg ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga
    23581 cggaatcggc gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt
    23641 gattcgctcg cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg
    23701 actcaaaaaa ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa
    23761 ttcacgaaaa ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt
    23821 ctgctcccac cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc
    23881 cacgctctta ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga
    23941 accctagctg tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt
    24001 ttaaggtatt cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg
    24061 atggagataa ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt
    24121 gctgaatata tttcgttctc agaatctaat acccaccata aatagccata tctcatattt
    24181 tgtaagtctt ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt
    24241 acgatttgtt ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga
    24301 tcacttgccg ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac
    24361 cagacattgg ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca
    24421 gcataataat tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa
    24481 tcaacagttg ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca
    24541 gtttgatagt tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt
    24601 tttgtccaat cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt
    24661 gctttcattg ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc
    24721 actttatact ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc
    24781 tctaaagcaa tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa
    24841 gtatcgtctt ttttataacc attccattca tctgtataaa cttctacccc atccttatat
    24901 gcgacaattt gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta
    24961 ttcataagat attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa
    25021 ttttttcgat tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga
    25081 tggattctct gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac
    25141 ttcgacttct ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt
    25201 agagtccttg tcttgattag aggtgtataa agctttttat tagacatttt taaattccat
    25261 tacaatttgc ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta
    25321 ttttgtgatt aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat
    25381 attcattata ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact
    25441 gaagtttcgt ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat
    25501 tatttcagtg atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg
    25561 aatgatcagt gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat
    25621 catggtagtg acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc
    25681 atttgttctc ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt
    25741 acgcgtaagt cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc
    25801 aatccaaaat tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga
    25861 taactattat gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc
    25921 tcatatcgaa gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa
    25981 attgtttttg aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt
    26041 cgaagatgaa agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt
    26101 caaacgtttg tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat
    26161 gttattcaca gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt
    26221 gcggatcaca aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac
    26281 agccgaagca aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt
    26341 aggtaaattt attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa
    26401 aggcgatatg tcgatcgtag gaccacgtcc tgaacgttct ttcttcgtcg atcagttcaa
    26461 tgcagaaaac ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc
    26521 acaagtctat ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta
    26581 tatcaaaaaa tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct
    26641 atttgataaa atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga
    26701 aattgctttt ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc
    26761 gaaattcagc ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga
    26821 tattctaaaa gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac
    26881 agatcaaaca caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat
    26941 tactacgacc aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg
    27001 agaatatctc ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta
    27061 tgatcatttg ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg
    27121 tacaatggac gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta
    27181 tctaactcac gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta
    27241 tgttgtatgg aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa
    27301 aacttataat agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca
    27361 agtgatcatg aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac
    27421 taatcaatac cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac
    27481 tgtaacagac gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc
    27541 agttattttt tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc
    27601 aaagcagata ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc
    27661 ttcagcaaaa aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc
    27721 tgtaaaagtt ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg
    27781 agtatattag ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat
    27841 gggtttaggt ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa
    27901 ctatcaattt gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga
    27961 gattgaaaga ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa
    28021 atatttaata gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca
    28081 ggtttatttt ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag
    28141 aattgctcat agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt
    28201 gcgtaaattt ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca
    28261 agaagctggt gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga
    28321 tttagagctt tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat
    28381 ctcaatgagt acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc
    28441 ttatttgtta gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt
    28501 agtaggtgaa gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga
    28561 gaaaagagta aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga
    28621 tgtttttgta atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc
    28681 agcaaaatta aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa
    28741 tgtggatttt gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga
    28801 gccgtatgct aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga
    28861 acaaatggag agtatatata gtagagatca ttaaaaatgt tcaagatatt ttaggagtga
    28921 ttatttttga aaatatgtta tttgatattg gcatacaatg atattggcaa cttaaagaga
    28981 ttggtcaatc gattgaacga aaatgcagtt tttattattc atatagataa aaaaactgat
    29041 atagaaccat ttattgaagc atttaaatca tatgataatg ttgaattttt atgtaataaa
    29101 aaccgtgtta aaatttattg gggtggtttt tccattgtac aagcagaaat caatttagta
    29161 aagagagcat tacaaaatga gaaatattta aaatatgtat tattatctgg tgctgattat
    29221 ccaatcaaag ataatgagta tatttataat tattttaaaa agaatagttc agtggagttt
    29281 attcgtggaa tagatctaga tcaaattaaa cataaagaat tttattataa acatatagat
    29341 gtatatcaaa aacatgatta tcctagaatt aataggaata acacaactgc atttaaaatt
    29401 tttagagcta taattaatcg ttgtctgagg atgattaaac ttcccccaaa aattagacac
    29461 cataaatttg atttgtatca tggttctcaa tggtgggctt tgtcgaaaga atgcctaaca
    29521 gaattaatac aaatgtatga acaaaatcaa caagactacc tgaattttaa aataggtatg
    29581 tttgccccag atgaaaaatt ttttcataca ctttttttta attcctcgtt caaaaataaa
    29641 aatgttattg gaggaccaga tttaccactt gaattgaaaa atattgaaga aactacttta
    29701 caaacttcga aattagcaaa tattcatatt attgatcctt ccatgaacaa atggtttgat
    29761 gaaagggata tagatcaaat aaaagaaagt gaaaaattat ttgtaagaaa agttaaatcg
    29821 gattactctg ataaactatt aaatagaatt gataaagaaa ttttgaaagt tggaaatata
    29881 gatgaataga atagtacctg ttttagagaa gagtttatta agtcgagatg cgtttcgagt
    29941 aataaattta atcatttgtc tgctttattt tatgcctagt acttttaatg taactaatat
    30001 cccgatgaaa atagcttttg cttggggtgg aattatagtt ttgtatgagg tttttataaa
    30061 gaaaaatttt tttaaaatgc gtttttcttg gctactgatt ggagcaatga ttagttttat
    30121 cttaactata atcattaaca gagaaaattt tttaattcca tctatttata attttggata
    30181 tttgctgatt actttgatag taatttttcc tgcagacttt tcacaaagtg aagaggaaaa
    30241 aaagaaaaaa atggtgtatt ttaataatgt tttcattatc ataatttttt tagctgcgct
    30301 aatatctatc tatcaattta tttttttgat ttcttatcat gtcccaacag gaacgccagg
    30361 attacttgca agacaaggtt ttattgaaca tcggttattt ggtgtctata caagtccaaa
    30421 tgttggagct atgtttggat atacttctat tattttaagt ttaattagtg tattaatttc
    30481 aaaaataaca aaaacgattc gtgtttttta tatgatgaat tttatcgtac aaatatttta
    30541 tattacactt tcttcgtcta gaggtgccca ggtagtaatt tgttcttttt tagtattatt
    30601 tcttttgttg tttgccaatt caaaatttag aaagagcatt agagaagtac tacctgtaaa
    30661 aatcaaatgg atggtaagca tattattagt tctattggta tatttttctt ttggtactaa
    30721 ttatgctaag gattttttag caacaattcc tgtatcgatt gatagaaaag tagaagttat
    30781 agagtcaaat gaatcagata aaactccact tccagaaaaa gaagtaatat tacagcattc
    30841 tagtgataac tccgaaattt cagctggtcg ttttacgatt tggaaagctg cgcttaaagt
    30901 tataagacca actttgttat ttggttatgg agagaccgat ttctatggga atagcgatgc
    30961 aaaatttttg aaaaaagtag atcttgatac tacagatgtg aatgaactaa aaagagcaca
    31021 tggaaacatg cataatggat ttcttcaagt acttgtatca agtggtataa ttgcttttat
    31081 atgtatctat gctttctatg tgttaaatat ctttagttta ctcaaaacat tttttagaag
    31141 taaagtgaat tatgcgtttc ttggtattgt tttaattttt atactatcta tttttataga
    31201 tgaaatggtg gaggctcatg tacttttcaa taagcgcgat gtcatttcaa ttgttttctg
    31261 gtattatttg ggaagcattt cttacttgta tttaaaaaag gagaattaat atgaagacag
    31321 ctttcattgc ttggactcct ttacatttga ttaatatatt aaatgtttat caaaattttt
    31381 accgagaatc aaaaggagat ttatttattt attctgaatt tgaaaacgct agaagatata
    31441 gtgagcgtat aaaagaatta gggctattta atgatgttgt ttttgtgaat caccggaaaa
    31501 tcggttcttc ctttaaacgt aaaattaact tgttaactaa ccataatcaa tttttctcgg
    31561 atgatttatc cgtttatgat gaaattttta tacaggggga aaattatttc acaaaaatac
    31621 ttttctccaa tgtttacaaa aaaaatccag atgtaaaact aaattatata gaagatggtt
    31681 tgggtactta tttgaagaag agtatatttt caacaagtaa aaacgttcaa agagttttta
    31741 aaatattaaa taaaaatagc atcttcaaaa aaaaaattga cgattatttt gtctattgtc
    31801 cggaattgtt actttatgat gtaaaaaata taaaaaaaat tcccaaaata gatgaaaaca
    31861 gtaaattgat gaaaaacata caatcaatct ttgggataga agaagttttt gtcggagata
    31921 aatgtatctt tttt
//
LOCUS       DAZUMB010000018        31475 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000018 DAZUMB010000000
VERSION     DAZUMB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.018"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(353..2080)
                     /locus_tag="ACG80L_000713"
     CDS             complement(353..2080)
                     /locus_tag="ACG80L_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS5022993.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2073..3266)
                     /locus_tag="ACG80L_000714"
     CDS             complement(2073..3266)
                     /locus_tag="ACG80L_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5022994.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3453..4097)
                     /locus_tag="ACG80L_000715"
     CDS             complement(3453..4097)
                     /locus_tag="ACG80L_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5022995.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4191..4325)
                     /locus_tag="ACG80L_000716"
     CDS             complement(4191..4325)
                     /locus_tag="ACG80L_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS5022996.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4327..6459)
                     /gene="feoB"
                     /locus_tag="ACG80L_000717"
     CDS             complement(4327..6459)
                     /gene="feoB"
                     /locus_tag="ACG80L_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS5022997.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6456..6929)
                     /locus_tag="ACG80L_000718"
     CDS             complement(6456..6929)
                     /locus_tag="ACG80L_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS5022998.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7330..7554
                     /gene="nrdH"
                     /locus_tag="ACG80L_000719"
     CDS             7330..7554
                     /gene="nrdH"
                     /locus_tag="ACG80L_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS5022999.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7713..9872
                     /gene="nrdE"
                     /locus_tag="ACG80L_000720"
     CDS             7713..9872
                     /gene="nrdE"
                     /locus_tag="ACG80L_000720"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS5023000.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9922..10887
                     /gene="nrdF"
                     /locus_tag="ACG80L_000721"
     CDS             9922..10887
                     /gene="nrdF"
                     /locus_tag="ACG80L_000721"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS5023001.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10976..12115)
                     /locus_tag="ACG80L_000722"
     CDS             complement(10976..12115)
                     /locus_tag="ACG80L_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5023002.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12424..13137)
                     /locus_tag="ACG80L_000723"
     CDS             complement(12424..13137)
                     /locus_tag="ACG80L_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS5023003.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13391..14092)
                     /gene="nagB"
                     /locus_tag="ACG80L_000724"
     CDS             complement(13391..14092)
                     /gene="nagB"
                     /locus_tag="ACG80L_000724"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS5023004.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14326..15858
                     /locus_tag="ACG80L_000725"
     CDS             14326..15858
                     /locus_tag="ACG80L_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5023005.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16049..17251)
                     /locus_tag="ACG80L_000726"
     CDS             complement(16049..17251)
                     /locus_tag="ACG80L_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5023006.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17333..18022)
                     /locus_tag="ACG80L_000727"
     CDS             complement(17333..18022)
                     /locus_tag="ACG80L_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5023007.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18128..18496)
                     /gene="rplL"
                     /locus_tag="ACG80L_000728"
     CDS             complement(18128..18496)
                     /gene="rplL"
                     /locus_tag="ACG80L_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS5023008.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18549..19052)
                     /gene="rplJ"
                     /locus_tag="ACG80L_000729"
     CDS             complement(18549..19052)
                     /gene="rplJ"
                     /locus_tag="ACG80L_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS5023009.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19256..19383)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19462..20151)
                     /gene="rplA"
                     /locus_tag="ACG80L_000730"
     CDS             complement(19462..20151)
                     /gene="rplA"
                     /locus_tag="ACG80L_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS5023010.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20255..20677)
                     /gene="rplK"
                     /locus_tag="ACG80L_000731"
     CDS             complement(20255..20677)
                     /gene="rplK"
                     /locus_tag="ACG80L_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS5023011.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21020..21688
                     /gene="sdaAB"
                     /locus_tag="ACG80L_000732"
     CDS             21020..21688
                     /gene="sdaAB"
                     /locus_tag="ACG80L_000732"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS5023012.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21701..22573
                     /gene="sdaAA"
                     /locus_tag="ACG80L_000733"
     CDS             21701..22573
                     /gene="sdaAA"
                     /locus_tag="ACG80L_000733"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS5023013.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22664..23764)
                     /locus_tag="ACG80L_000734"
                     /pseudo
     CDS             complement(22664..23764)
                     /locus_tag="ACG80L_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(23856..24743)
                     /locus_tag="ACG80L_000735"
     CDS             complement(23856..24743)
                     /locus_tag="ACG80L_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5023014.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24820..25365)
                     /gene="nusG"
                     /locus_tag="ACG80L_000736"
     CDS             complement(24820..25365)
                     /gene="nusG"
                     /locus_tag="ACG80L_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS5023015.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25466..25636)
                     /gene="secE"
                     /locus_tag="ACG80L_000737"
     CDS             complement(25466..25636)
                     /gene="secE"
                     /locus_tag="ACG80L_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS5023016.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25660..25812)
                     /gene="rpmG"
                     /locus_tag="ACG80L_000738"
     CDS             complement(25660..25812)
                     /gene="rpmG"
                     /locus_tag="ACG80L_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5023017.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25974..26618
                     /gene="cbpA"
                     /locus_tag="ACG80L_000739"
     CDS             25974..26618
                     /gene="cbpA"
                     /locus_tag="ACG80L_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS5023018.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26674..27177)
                     /locus_tag="ACG80L_000740"
     CDS             complement(26674..27177)
                     /locus_tag="ACG80L_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5023019.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27190..27236)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27774..28739
                     /locus_tag="ACG80L_000741"
     CDS             27774..28739
                     /locus_tag="ACG80L_000741"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5023020.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28838..30010)
                     /locus_tag="ACG80L_000742"
     CDS             complement(28838..30010)
                     /locus_tag="ACG80L_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023021.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29997..30122)
                     /locus_tag="ACG80L_000743"
                     /pseudo
     CDS             complement(29997..30122)
                     /locus_tag="ACG80L_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            30230..31309
                     /locus_tag="ACG80L_000744"
     CDS             30230..31309
                     /locus_tag="ACG80L_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5023022.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT         9596 a         6510 c         5686 g         9683 t
ORIGIN      
        1 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
       61 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      121 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      181 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttggacgt ttcgaagatg
      241 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
      301 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
      361 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
      421 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
      481 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
      541 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
      601 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
      661 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
      721 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
      781 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
      841 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
      901 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
      961 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1021 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1081 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1141 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1201 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     1261 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     1321 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     1381 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     1441 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     1501 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     1561 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     1621 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     1681 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     1741 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     1801 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     1861 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     1921 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     1981 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2041 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2101 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2161 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     2221 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     2281 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     2341 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     2401 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     2461 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     2521 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     2581 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     2641 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     2701 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     2761 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     2821 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     2881 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     2941 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3001 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3061 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3121 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3181 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     3241 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     3301 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     3361 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     3421 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     3481 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     3541 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     3601 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     3661 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     3721 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     3781 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     3841 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     3901 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     3961 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4021 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4081 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4141 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4201 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     4261 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     4321 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     4381 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     4441 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     4501 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     4561 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     4621 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     4681 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     4741 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     4801 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     4861 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     4921 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     4981 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5041 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5101 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5161 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     5221 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     5281 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
     5341 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     5401 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     5461 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     5521 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     5581 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     5641 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     5701 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     5761 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     5821 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     5881 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     5941 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6001 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6061 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6121 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6181 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     6241 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     6301 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     6361 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     6421 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     6481 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     6541 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     6601 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     6661 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     6721 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     6781 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     6841 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     6901 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     6961 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7021 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7081 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7141 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7201 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     7261 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     7321 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     7381 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     7441 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     7501 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     7561 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     7621 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     7681 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     7741 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     7801 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     7861 ttctatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     7921 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     7981 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8041 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8101 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8161 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     8221 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     8281 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     8341 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     8401 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     8461 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     8521 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     8581 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     8641 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     8701 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     8761 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     8821 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     8881 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacggat atctcatgta
     8941 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9001 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9061 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9121 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9181 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
     9241 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
     9301 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
     9361 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
     9421 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
     9481 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
     9541 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
     9601 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aatggcacaa caacacgtcg
     9661 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
     9721 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
     9781 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
     9841 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
     9901 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
     9961 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10021 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10081 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10141 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10201 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    10261 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    10321 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    10381 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    10441 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    10501 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    10561 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    10621 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    10681 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    10741 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    10801 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    10861 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    10921 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    10981 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11041 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11101 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11161 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    11221 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    11281 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    11341 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    11401 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    11461 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    11521 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    11581 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    11641 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    11701 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    11761 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    11821 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    11881 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    11941 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaactgcc gaaagcattt
    12001 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12061 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12121 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12181 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    12241 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    12301 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    12361 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    12421 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    12481 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    12541 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    12601 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    12661 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    12721 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    12781 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    12841 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    12901 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    12961 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13021 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13081 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13141 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13201 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    13261 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    13321 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    13381 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    13441 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    13501 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    13561 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    13621 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    13681 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    13741 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    13801 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    13861 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    13921 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    13981 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14041 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14101 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14161 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    14221 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    14281 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    14341 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    14401 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    14461 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    14521 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    14581 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    14641 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    14701 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    14761 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    14821 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    14881 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    14941 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15001 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15061 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15121 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15181 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    15241 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    15301 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    15361 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    15421 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    15481 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    15541 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    15601 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    15661 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    15721 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    15781 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    15841 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    15901 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    15961 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16021 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16081 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16141 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16201 caataaagta cataaacata aatacagtat tatttcggtt gcttgctgct tcacctagcc
    16261 cttgtactcg tgtttgattg gcgacttgac caaattgatt cccgacgtct aataaaataa
    16321 tagctaaaag caaaatggcc aaatggcttc cgccaaccaa taaagcaata aagcttattg
    16381 tttgcataac aagtcccata tacacgattt ttctttcaga ataagaatct gccagtcgtc
    16441 ccacgatagg tgcagcaact gctccagaca aaccaaaaat cgctaatatc cctgcttcga
    16501 atgttcccca attgtaggcg ggactgctga tataaaaaat caacgtagac caaaagatcg
    16561 aaaatgttcc aaacatgaaa aatccactga ctgccgcttc tcttaataga cgttgagaag
    16621 caactaaatg cggcaaactt ttaagtgaat caaggtatga aacagctgta ctactttgct
    16681 ggacagaacg cggcattttc cagtaaagca gaagtgtaag aattccgact gcacataaag
    16741 caaataaata gatcatccgc caagaaaaaa gactggcaac aataccagat accgtccgag
    16801 aaagcaaaat ccccacgagt aaaccgctaa gcaatgtacc catgaccttc cctcttgctt
    16861 ttggtcccgc tagtaccgca ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc
    16921 ctattaaaaa agaagcgcat gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca
    16981 gagagacggc agataacgct gccattcgaa tgatcaattt tggacgatcc accacgtccc
    17041 ctaaaggaac taaaaacagt aaccctaacg catagcctaa ttgggtcaac atcgtcaata
    17101 tcccgactgc acttgtgcta acagataaac tagctgctac ttttgttcca atcggctgga
    17161 tgtaatacat attggcaaca acaacgccac aagtgattga taatagtaaa ataagcgaag
    17221 ttgttaaagg ttttttttca ttactcttca ttgatttccc ccatttccta tcttagttta
    17281 caacttatgg aaaagattgg aaatagaaaa atacaactat tcgccacact tattatttgt
    17341 gcttttgttt tccttgtttt ttcttgcgat ctttataaat gaagacaact aaccctacta
    17401 gcacagtaac acttgcaacc aacgtgactg cagttttgtt atatccgtga aacagagctt
    17461 ctccaccaca cgcatacaac acagacgaag ggatgactcc aatcacgatc gacaatatga
    17521 tttgctgtag aggcaatttc atcgcatctg cagcaaaatt gaccatagaa gatggaataa
    17581 ccggtatcat atagcctaac attacaccta tttttggatg tttcatttgt cggattgctt
    17641 gcacccaatg attcgtttcg gttttcttat ctaaaaattt taagcgatgc ataaggaaaa
    17701 ttgcagtgat gttaccaaga gcattaccca aaatgttgat gatcgcacct gtcagtggtc
    17761 caaagctcaa accagcaatg actccaacta ctgaagtagg aatacctgga acagcacaca
    17821 tcaccgctgt caatacgatc aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg
    17881 cgagtagctc tgccttactc gctctcggat ttaaaaataa ttggatgtct gtctgatatt
    17941 ctaaatacaa ccgatagccc actaccagta tcagaatgat tcccaacgag atcaatatca
    18001 atcggattat tttctttttc atatttcctc ctgaaaaaaa tctatattca ttaaataata
    18061 gtttaacata aaaacaaaaa agcaccgaaa tccaacatag gatctcagtg cttttaaata
    18121 gtaaaaatta tttaacagtt acagatgcgc caacttcttc aagtttagct ttcaattctt
    18181 cagcttcttc tttagatacg ccttctttaa ctggtgcagg agcgccatca actacagctt
    18241 tagcttcttt caagcctaaa ccagttgctt cacgaactgc tttgataact ttaacttttt
    18301 ggtctccagc tgcagttaat tctacagtga attctgtttg ttcttcagca gcagcagcgc
    18361 caccagcagc agctacagct acaggagcag cagcagatac gccaaattct tcttcaatag
    18421 ctttaactag gtcgttaagt tctaggatag ttgcttcttt taactcagca acgatgtttt
    18481 caatgttcaa tgccattata atttcctccg tttaattgtt cttttttatt taatgcttta
    18541 tgcggcaatt aagctgcatc tccatcttct ttgtctgcca ctgctttgac agcgtaagcc
    18601 acgttgcgga ctggcgcttg tagtacagat agcaacatag atagaagtcc ttcgcgattt
    18661 ggcaattttg ccaatgctgt gatttcttcc aatgaagaga ctttaccttc gatgataccg
    18721 cctttgattt ctaatgcttt tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg
    18781 actacatctt cgttactgaa tgcaacagct gtaggtccag tgaatacttc gtccatgcct
    18841 tcaaggccag cttttttagc tgcacgtgaa agaatagaat ttttgataac tttcatttct
    18901 acgttagcat cacgaagttg tttacgtaaa cgtgttactt catcaactgt taatccgcgg
    18961 tagtcaacga ctactactga tgcagctgct gtaaattttt cgcttacttc atcaacgatt
    19021 gctgcttttt ttgcgataat tgcttcactc atttttgagt ttcacctcct gaattttgat
    19081 ggaagagttt tttgcactga aaaggcaagt cacttttata gttactttgc tcaaccagta
    19141 ttattcatca cctattcact tcgttcatag tgattctact cgctttgctc gaaaggaaac
    19201 tcctttcgct ttgctcaacc agtattgttc atcatctctt cacttcgttc atagtgattc
    19261 acaataaaaa actctatgcc accgcagaca tagagggact attgaatata ttcaataaac
    19321 gtcctcggta ggaaattaag gcttgatgcc acctactgtc ttcggtacaa gttaattatt
    19381 aacctcaact agcttagcat gctaaactag ttgagtcaag aattattttt cagttggatc
    19441 aatcaacatt ctaatattta attagaaaga agcttggtca acatggatac caggtccaaa
    19501 tgttgttgtg atcgttacat ttttcatgta ttgaccttta gctgctgatg gtttagcttt
    19561 tagcaataca tcatggattg ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac
    19621 ttttccgatt ggtacatgga tatttccagc tttgtccaca cggtaagtta ctttaccagc
    19681 tttcacttcg tttactgctt ttgttacgtc cattgtaact gtaccagttt tagggtttgg
    19741 cattaagcct tttggtccta atactcgtcc tagacgacct acagtagcca tcatatcagg
    19801 tgttgcaaca actacatcaa agtcaaacca tccgttttgg attttttgaa ccatgtcgtc
    19861 gtcacctacg aaatctgcac cagctgcttc agcttctttt gctttgtctc ctttagcaaa
    19921 aactaaaaca gtttgtgttt taccagttcc gtttggtaat acaacagcac cacggatttg
    19981 ttggtctgct tttttagggt ctacgttcaa acggtatgca acttcaactg ttgcatcaaa
    20041 ttttgcaatg tttgtttctt ttgctaaagc tactgcttcg gcaacaggat aaactttact
    20101 tgaatcaact ttttttaatg catcttgcat ttttttgctc tttttagcca ttttggttcc
    20161 tccttgattg tggttataac ggttgtacct cccacgtgtt ctatatcttt caatatagag
    20221 ccaactgaga agctgctgtc ttagggtgta tagattattc tacagtgatc cccatgcttc
    20281 gtgcagttcc ttcaaccatg cgcattgctg cttcaacgtt agctgcgttt aggtcttcca
    20341 tttttaattc agcgatttct cttacttgat cgctagaaac tttcgcaact ttatttttgt
    20401 ttggttcgcc tgaacctttt tcgattttag ctgctttttt caatagaaca gcagctggcg
    20461 gtgtttttgt aacgaatgtg aatgaacggt cttcgtatac agaaataaca accggaatga
    20521 tcaaacctgc ttgatcagct gtacgagcgt tgaattcttt tgtgaatccc atgatattga
    20581 tacccgcttg acctagtgca ggacctactg gtggagctgg agttgcttta cctgcaggaa
    20641 tttgcaattt aacgattttt tctactttct ttgccacgag acatacctcc ttaagtccgt
    20701 gatgtggtta attggagatg catatttctc ctcccacgtt ttatctgatc tgaaaaaatc
    20761 cagacaaata atgtgcaaaa caatgcacac tggtacagta tagcaaattt atttgttttt
    20821 tcaagatttt tttctgctat ttttacattt tgcttgaata aacgctaaat ttattctaaa
    20881 aaaacaacaa tttttcgttc tcatttcttc ttttatgtta cacttgcgct agcatggttc
    20941 aacagatgaa agcaaacaat aacacggttt tttagaccct tgtacaaaga ttcctacata
    21001 acaaaatgag gtggagaaaa tgaaagattt aaggtataga agtgtctttg atattattgg
    21061 acctgtgatg attggtccaa gtagctctca cactgccgga gctgctagaa tcgggaaaat
    21121 catacgtagt atatttggcg aacagcctga ttctgtagat atttatctat atgaatcatt
    21181 tgcaaaaacc tatcgggggc acggaacaga tattgcatta gttggcggcc tgttagggat
    21241 ggaacccgat gatgaacgtt tagctgattc tttgaaattg gcatatgaag ccggtatgga
    21301 agtcttgttt gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa agatgcttct
    21361 aaaaaaagga acacgaaagc tttcagtcac tggcatttcg atcggcggtg gcaatatcca
    21421 gatttctgaa ttgaatggat tcaaaatttc tttaagcatg ggtactccta ccttgatcgt
    21481 tgtccaccaa gatgttccag gtatgattgc taaagtgact agtatacttt ctgagaccaa
    21541 tacgaatatc ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat
    21601 aattgaagta gatgatcctc aagtagaaga aacggtaaaa agactcaaac agctgccaaa
    21661 tatcgacagc gtaaattatt tcgattaagg agctaaaaaa atgtttttat cgattgaaga
    21721 attagtagat caagcaaaag cttatccaag tgtagcagaa ttgatgattg cagttgaaat
    21781 ggaaatgagt ggccgttccc gtagtcacat catcgagatc atggaaaaaa acctctcagt
    21841 aatggaacaa tcaatcaacg agggaaaaga aggtgttacc tccgtcacag gaattaccgg
    21901 tggtgatgca aaaaaattag atgattattt aaatgaggca gactttttaa gtggagaaac
    21961 aatcttaaaa gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt
    22021 gatttgtgct acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt
    22081 ccgagatcgg cttgatttga cacatgaaca gcaattggaa ttcttattta ccgctggcgc
    22141 tttcgggtta gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg gatgtcaagc
    22201 agaagttggt tctgcaagtg caatggcagc agcagctttg gtttgtgcaa aaaatgggac
    22261 tcctcatcaa gcagcacaag cagtagccat tacattgaaa aatatgatgg gactgatttg
    22321 cgatcccgtt gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc
    22381 tcaagctttt atctctgctg atatggcatt agcgggtatc gaaagtgtca ttccgccgga
    22441 tgaagtcgtt gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac
    22501 agcagaaggt ggcttagctg ttacgccaac tgctaaacga cttgcggaag agattttaaa
    22561 taagagggcg tgataaagct ccttaatttc gagtattaag agattagtta tcttcataac
    22621 aaaaaacgaa gagaggacta attgcccttc tcttcgtttt ttattaatcc atcaatttcg
    22681 tattttttac ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc gacaattctt
    22741 tgttcaggtg ttcaaaaaca gatgttactc cttctgcccc acctagattc aacccataaa
    22801 tcactggtcg gccaatcgca actaagtcag caccacttgc cagagctttg aagacatgct
    22861 ctcctcgacg gacaccagag tcgaaaatca ctggtacacg tttgttgact acttcggcaa
    22921 tttttggcaa tacttcaaag gacgcaggac ctccatctag ttgacgtcca ccatgattgg
    22981 atacccagat accatctgcc cctgcagaga tagcgattac tgcatcttct ggcgattgta
    23041 ttccttttac gataactgga agatgggtga tttctttgat tgttttgatg tcatctggtg
    23101 tcaagccttg ttttgcagct gcataaattt ctgcgattcc ttttccttcg ccattaccac
    23161 ttgcactttg ttcgctatac gcagctaagt taggcattgg taatggaaat tggaattgat
    23221 tgatgacgtc ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa atgatagctt
    23281 tagcccctgc ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg tctttactca
    23341 tatataattg gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg
    23401 tatttgcata agtgctgatg gaaaagattg atccagcagc agctactcct ttagctgtat
    23461 cagcttcccc tttttcatgt gccaatcctt gggccgctga tggcgcttgg ataatcggtg
    23521 tatctaattc aattccgaaa acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata
    23581 tccgtggcat gattttcttt gtattaaaag aagtcgtatt ttctttcatc gtccactcat
    23641 cttcagaacc accgcgaata tagccaaaag ctcctgcttc catgcgctct tttacacgac
    23701 cttctagtta ggcaatgttt acaatatcga ttggatgttc ttcattactt gtatgataag
    23761 tcatagtaga tccttctttc atcttcttga ttacatataa atcatactaa acttacaaaa
    23821 agtaagcaaa catttactct cagatacttt tatcgctatt ctttccaaat aaatcgactt
    23881 acaagctgat ctacttcttg gttttcatga agcatactat gagaggcatt ttcacctgtc
    23941 actacttttt cctctatttc atttccgcgt tgcttcaaca acgcatagac cgctaatgca
    24001 ctgtttaacg gtactgttcc atcactcaag tctgtttcat ctagttgacc agcaatcaag
    24061 aaaaatcgag tggttatagg aatattaccg atcatttgct ggtaatcttg atagcggtta
    24121 gaaacgacag caggcccctt tgctaactca tctgtcagag actgcgcact atcatcgaca
    24181 aaatcattaa aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc
    24241 tgtccatacg ttcctaaata gcgtagtgca ctaactcctc ccatggaatg ccccactatg
    24301 ttgactttat ctatgccgta gttttctttc agatacaaaa gacaagtttt gatccattct
    24361 gcttgattcc actcgttatt tttattatct tcaaacaata cttggaccat cggattgtct
    24421 gcttttccag acaaatctcc tgttgcttgt atttgtccat ctgttcctac tgttaaaacc
    24481 aattctttta ctgcttgctg atttttttcc aaacgccgca acatcccttt aaaggaattt
    24541 tctgtcccgc tgtagccgtg gaaaaaaagt gttggaatag tggttttttc tatcacttta
    24601 ctggattctg ccgttttttc tttatcagaa acttcagtag tatttatctt cgtcgtctgg
    24661 caaccactta acgcaaacaa actgataagg actaaagctg ctgcagttgc acgtttgcat
    24721 aaatttttga ttggttctgt catacacctc ttcctttcca tatagaaaaa agaaagactt
    24781 gaaaggccaa tcatatgatc gacatttcaa gtctctttat taatcgattt tatctacttg
    24841 atcgaaatca agttctgtac tggtttcacg tccaaacata tcgatgttga ctttcaattt
    24901 ttctttttct ccgtcaactt ctgttacttg tccttctaat ccagcaaatg cgccttcgat
    24961 gattttcacg acttcgccaa cttctacttc tagatcattt tgtcgcaggc tcataccgat
    25021 agaacgcaag atatgattga cttcttctgg aagtaatggt gctggtttgc ttccggctcc
    25081 gtgtgaacca acgaatcctg tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc
    25141 tgtcatgatc atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga tttctttttc
    25201 tttcccattt ttgacttcag tttctttttc ttcaggaaca acgacacgga aaataaagtc
    25261 ggccattttc atactttgcg cacgagattc gatatttgct tttactttgt tttcatatcc
    25321 tgaatatgta tgcaagacat accagttttt ttcaaaagat tccattgaag gctcctcatt
    25381 tcattgtaaa ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt cattatagca
    25441 ctaaaagtac gttgtggcta gcgagttatt ttaaaatcca gccaaataag ctttggatgg
    25501 ctgtatccat tacgtaaaac atcaatccga aaataataga agtttcgatg actactaacg
    25561 tatctttacg gagttgtttt ctgcttggcc aagttacttg tttgatttca tcttttacgc
    25621 tttttagaaa tttcatgaga caacctccct tgctactgtt tatttcgttt ctttatgtaa
    25681 tgtatattga ttacaatatt tacaaaactt attgatttcc aaacgttcgc cgctttttcc
    25741 ttcactgact gattttgtgt agttgcgaga cccacaaacg gaacaagcga gtgctgcttt
    25801 tttctttgcc atttacttct gcccccttgc tgagaatcag acttatactt cataacagta
    25861 tcacgtaact ttttacttgt caatttgtga aataaactat ctattgtttt ggataaaatt
    25921 atgatatgat taatttgaaa ttaagctaaa atgagacaat tggaggtata gcgatgctat
    25981 taaaaacact tgtatataag aaaaaagatc tgacaaccgt tacagagact agtacattag
    26041 aagaagcatt aaaaatttta gaaacttccg gataccgttg tgtgccgatc ttagattctt
    26101 caggtaatat tttccgtgga aacatttaca aaatgcatat ctatcgtcat aaagcaaatg
    26161 gcggtgatat gacgcttcct gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc
    26221 tggatacttc tttcttcaaa gtattcttta cgatcaaaga gcttccttat attgctgtcc
    26281 ttgacagtga aaatcatttt tatggtattt tgacacatag tacgttgctt aatatgctct
    26341 ctcaatcttg gagcgtagaa caaggaagct atgtactgac aattgcttcc acaggaaagc
    26401 aaggagattt ggctgctatt gcaaaaatca tttcaaaaca cagcagcatt gccagctgta
    26461 ttactcttga tatcgaaaaa gacgaataca ttcgacgtac attgatcacg ctaccggcta
    26521 acactgaagc gaaagtatgt gaaacgatcg tcaaccactt agagaaaaag aattataaag
    26581 tggtagaaat tgaaaatttg caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac
    26641 agctaatctg ctttcttttt tcgcttttta taattagaca cgcttttcaa aatcaagcgc
    26701 gcgatctaaa taatacatca atggtgctga aacagccatg acgataaact cacttaatgc
    26761 aagcgtgcca taggttgtcc aaaatgcttg gccacctgtt ggaactaaca tataagcaat
    26821 cagtcccatg ctgatggtaa agaagatcgt gttcaatact aaacgcatct ttacattttt
    26881 cactttgttt tgcaataaag ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa
    26941 aaccacatcc atgataccaa aaccaaagaa ggcattatag acgatgaccc cgcctaaaac
    27001 tccccaaagc aatttacggt taaagacaac taaatgattc aaactttctg aaatacgaaa
    27061 ctggatcggc cctgaggata caggcgcaac taaaatagtt aacgctagat aaagagccat
    27121 gatgatgcca tttacaacga ttacggataa ccgttgtctg cttttcgttt tttgcatttt
    27181 ctctcctctt ttctcctagt ttttttaacg cgggatggtt gatgaaccgc ggttatgtga
    27241 aacaaacaat aattataaca gacttttcat ttatttcaat ccgtcttatc tgcttttaaa
    27301 aaagcaaaag cttaaacaat gtaaacgttt gctaaaaatc gtgggatatg gtataaagta
    27361 agtgtaaggg gtcataagaa ttcccagtaa agcagacgaa gtaggatctg caagaacaga
    27421 attgttgtga aacttaaata aataaaaaag gatgtgggaa tttgagcaac ctcttactag
    27481 agaacggtct agcaagcagc tgaagatcta agtggacatg ccgaaccttg gaacttctcc
    27541 ttacgtaagt aatggatcat gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc
    27601 tttttcattt tcctagactt ctgctataat aaccaagaat taattttgta acagattttt
    27661 ttgaaaaaac aagtttcttt cattcaaaaa atagaaaaag cttgctaatt attggttaac
    27721 cagcaaacag gagtattaaa atatcttgtt ttattgaata gcaggaggaa atcatgaaat
    27781 tccattatca tatattgatc aatccatcgg caggcagcgg aaatggctat aaagtagcag
    27841 aaagaatttt accggtatta aaaaataagc atattgacta tacacttcat tatagtgaat
    27901 acaaaggaca cgatgctgag attgctgaaa ctttggcaaa agaaacattg attccttgga
    27961 tagaagaaga acagaaaaca atcgatactt tccccctttt gatcatcgta ggaggtgacg
    28021 gcaccttaca tcaggtactt gatactttct atcagatgga agtagaattc ccagttgctt
    28081 atattccagc aggttctgga gatgattttg ctcgaggggc aggccttcca aaaaatccca
    28141 aaaaaggact tcagctgatt ttagcagctc aatcaccaga aaaagtccac ataatggctt
    28201 atgaagaaaa aataagtgaa aaaaaaggaa tagctgttaa taatttcggt ataggattag
    28261 atgcagctat cgtccatgcg acaaatcatt cttccacaaa aaagcgattg aacaaataca
    28321 acttaggatc attttcgtat ttattctcta ttctccgtgc tttattcact caaaaaggat
    28381 ttccaattct ggtagatgca ggaggaaaac aattaagttt ttcaaacgca tttttatgta
    28441 cagcaacgaa gcatcctttt tttggcggag gaatcgcgat tgttccaact gctgatgcgt
    28501 ctaaaccagt cattgatttt gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc
    28561 tcattctttt acttatacag aaaaaacaat tgaaatcgaa atatttccat cactacacaa
    28621 caaacagatt acgtatcgtt tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa
    28681 tggaaaaacg accttttgat atcacaataa ccacaactaa acagttgttt tggtgttaat
    28741 aaaagaggtt ttgtcagaag ctacatctgg aacaccgttt attcggaaaa aaggactgtg
    28801 acacgacttt tgtcacagtc ctttttttac tatgcagtta attctttgcc caattgggtt
    28861 gccatcgata gtttctttct ttacccaaaa taaattggat cattgaaaac accgcagcaa
    28921 aagcaactaa gacacaacca ttgaaccaaa aagattctgc aaatgtttga ttgtaactga
    28981 gtgtttcact gatcaagtta ttattttgaa caaaccaata tgttggtgtg aatgccgcta
    29041 ttttgctaac catttctggc aagtattctg ctggtacaaa caccccaccg ataaagcaac
    29101 tgcctgtgat aaaaatatct ttgatcccac cgctggtttc tgagtttttt accaatgaag
    29161 tgatacaagc cgaaaagcta atgatcggca ataaaaacaa gaaagtattt aaaatatgat
    29221 accataccgt tatatcccac gaggtgtgga tcactaacag aaccattaga agtaagaagc
    29281 cactgaacaa cagcagcgag taactaatca aactaaaggt tactttgcga ttcaagtttc
    29341 gtttggtaat gggcgcactc tggtttcgtt tactgatctc tttgcgatta aatgctagat
    29401 tgatgacgct gaaaccgcta aaaatggata gaaacaaccc ataagacaag aaattaaagg
    29461 ttgtgcccgt caacagcttt ttcatccgct gactatatcc cgcacttagc tcaacttctc
    29521 cagtaaccga gagtgccatt tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt
    29581 cttgaaacag aatcagtgtt tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag
    29641 aaaaggcatc tgggcgactc tgtatcttga tcgtcggcat ctttcctact tcgacttgtt
    29701 cggtaaagtc tgcaggcaga tgtaagataa tgtttacacg attaaaataa agagcatcat
    29761 cgatttcttt ttgtgaggtt ccatccaatg taacgactgt ttccttgtca ttcaaatatt
    29821 tacgaaggcc atcgactaca ggcgactctt cttcactaat caaggcgatt ttcgcatctt
    29881 gcagagaggt attttcaaca tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga
    29941 taattacacc tatcagtaaa ttcgatttat ttgctttaag caatgtccaa atcgttttat
    30001 aaagcgtcat aacgcacccc tctttccatc caagcatttc ctaacgcaaa aaggaaaata
    30061 aatccgatca accatagaag attggtataa aaactagcca aatcagtgta gaaataaagt
    30121 ttaggcggat tttataatga acttagccat ttaaaataaa aaaaacgcca cgtgaattcc
    30181 atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca tgacgcaaac
    30241 ccatgataac acagtttcac gcaagggaaa acatctttcc tactcagaac gttgccaaat
    30301 cgctatcctg aaaaaggaag gctattccaa ccgtcaggtt gccaaagtgc tgggacgtgt
    30361 tccgcaaacc ataaacaatg agattgatcg cggaaccatc acccagttaa aacgccaaac
    30421 gcaaaatgga aaagtgtatg acacctacta ttctgtttat gacgctgatg caggacaggc
    30481 cttctatgaa aagcaacgac tgaactgtgg cagacgcccc aaatgggcga acacaaatgc
    30541 cttcattgat tgggcagatg acaaaatgtt aaatgaaaaa tggtcgccgg acgtcgtggt
    30601 cggatttgcc agaaaacacg agctgttcga ttgtgccatc attcctggca caaccgctct
    30661 ttatcaatgg attgaccgcg gaatcatgcg aacaaagaac atggacttgc ttgaaaaact
    30721 gtcccgtaaa atcaaagcgg tccgcccagg acaccgtccc aacaagcgtg ttctcgggac
    30781 gtcaattgaa gaacgcccta aagaagttga aacacgggaa acgttcggtc attgggaaat
    30841 cgacacggta gtgggaaaca agaccaagtc ggatgccgtg ctgctgacgc tggctgaacg
    30901 gcagacacgc tttgaagtca tcctaaagtt gaacgggaag gacaccgaat cggtggacca
    30961 agcggttcaa cagctaagag aacgggctgg cgctaacttc gatcaattat ttaagaccgt
    31021 tacatccgac aatggctcgg agttttctgg gctacacgat gctctgcaag atgtagctga
    31081 cgtctatttc agccacccct atgcttcttg ggagcgtggg acgagcgaaa accagcacaa
    31141 attaattcgc cgtttcgtcc ccaagggaag cccgattagt gaggtgagcg agaaccagat
    31201 tttgcggatc cagcgttgga tgaacgatta cccgcggaaa attttgggtt atgcgacacc
    31261 acatgataca ttcgtccagg cattcaagca agaacggttg gtggcttgac ccattattcc
    31321 ttgataacga tcctcactcg ggacatggct tcatgtctgg aaagctggcc tggggccaga
    31381 atcgtctgaa gactatcata cctcagttca ttatcaaggc ctcactgatt tagaataacg
    31441 aggcagttac ggtatcaaac aaatgagtta cctgt
//
LOCUS       DAZUMB010000019        31118 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000019 DAZUMB010000000
VERSION     DAZUMB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.019"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..376)
                     /locus_tag="ACG80L_000745"
     CDS             complement(<1..376)
                     /locus_tag="ACG80L_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023023.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFV"
     gene            643..2343
                     /locus_tag="ACG80L_000746"
     CDS             643..2343
                     /locus_tag="ACG80L_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS5023024.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2313..2531
                     /locus_tag="ACG80L_000747"
     CDS             2313..2531
                     /locus_tag="ACG80L_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS5023025.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2551..3441
                     /locus_tag="ACG80L_000748"
     CDS             2551..3441
                     /locus_tag="ACG80L_000748"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS5023026.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3459..3743
                     /locus_tag="ACG80L_000749"
     CDS             3459..3743
                     /locus_tag="ACG80L_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023027.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4230..4844
                     /gene="gmk"
                     /locus_tag="ACG80L_000750"
     CDS             4230..4844
                     /gene="gmk"
                     /locus_tag="ACG80L_000750"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5023028.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4849..5154
                     /gene="rpoZ"
                     /locus_tag="ACG80L_000751"
     CDS             4849..5154
                     /gene="rpoZ"
                     /locus_tag="ACG80L_000751"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5023029.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5407..7815
                     /gene="priA"
                     /locus_tag="ACG80L_000752"
     CDS             5407..7815
                     /gene="priA"
                     /locus_tag="ACG80L_000752"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS5023030.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7830..8321
                     /gene="def"
                     /locus_tag="ACG80L_000753"
     CDS             7830..8321
                     /gene="def"
                     /locus_tag="ACG80L_000753"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5023031.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8314..9252
                     /gene="fmt"
                     /locus_tag="ACG80L_000754"
     CDS             8314..9252
                     /gene="fmt"
                     /locus_tag="ACG80L_000754"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS5023032.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9252..10610
                     /gene="rsmB"
                     /locus_tag="ACG80L_000755"
     CDS             9252..10610
                     /gene="rsmB"
                     /locus_tag="ACG80L_000755"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS5023033.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10623..11363
                     /locus_tag="ACG80L_000756"
     CDS             10623..11363
                     /locus_tag="ACG80L_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS5023034.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11360..13429
                     /gene="pknB"
                     /locus_tag="ACG80L_000757"
     CDS             11360..13429
                     /gene="pknB"
                     /locus_tag="ACG80L_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS5023035.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13594..14493
                     /gene="rsgA"
                     /locus_tag="ACG80L_000758"
     CDS             13594..14493
                     /gene="rsgA"
                     /locus_tag="ACG80L_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS5023036.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14506..15156
                     /gene="rpe"
                     /locus_tag="ACG80L_000759"
     CDS             14506..15156
                     /gene="rpe"
                     /locus_tag="ACG80L_000759"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5023037.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15157..15795
                     /locus_tag="ACG80L_000760"
     CDS             15157..15795
                     /locus_tag="ACG80L_000760"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS5023038.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15950..16804
                     /gene="mreC"
                     /locus_tag="ACG80L_000761"
     CDS             15950..16804
                     /gene="mreC"
                     /locus_tag="ACG80L_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS5023039.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16808..17317
                     /gene="mreD"
                     /locus_tag="ACG80L_000762"
     CDS             16808..17317
                     /gene="mreD"
                     /locus_tag="ACG80L_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS5023040.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17572..19146
                     /locus_tag="ACG80L_000763"
     CDS             17572..19146
                     /locus_tag="ACG80L_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS5023041.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(19214..21013)
                     /locus_tag="ACG80L_000764"
     CDS             complement(19214..21013)
                     /locus_tag="ACG80L_000764"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS5023042.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            21136..21642
                     /locus_tag="ACG80L_000765"
     CDS             21136..21642
                     /locus_tag="ACG80L_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5023043.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            21721..22443
                     /locus_tag="ACG80L_000766"
     CDS             21721..22443
                     /locus_tag="ACG80L_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5023044.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(22521..23921)
                     /locus_tag="ACG80L_000767"
     CDS             complement(22521..23921)
                     /locus_tag="ACG80L_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS5023045.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(24091..25065)
                     /locus_tag="ACG80L_000768"
     CDS             complement(24091..25065)
                     /locus_tag="ACG80L_000768"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5023046.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            25418..25978
                     /gene="pth"
                     /locus_tag="ACG80L_000769"
     CDS             25418..25978
                     /gene="pth"
                     /locus_tag="ACG80L_000769"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS5023047.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            25995..29516
                     /gene="mfd"
                     /locus_tag="ACG80L_000770"
     CDS             25995..29516
                     /gene="mfd"
                     /locus_tag="ACG80L_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS5023048.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            29531..>31118
                     /locus_tag="ACG80L_000771"
     CDS             29531..>31118
                     /locus_tag="ACG80L_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5023049.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
BASE COUNT        10073 a         5726 c         6689 g         8630 t
ORIGIN      
        1 atacaaaatt attctgtagt ttataaaaaa acggcgaatg aagatgtgtt ttcatatgac
       61 caaaatataa taattctgaa atttctaaag gtgtcaattc tttcagcatc gttaaatcag
      121 taaagtcgat ccacttcaat tcatcgaatc cacgcctgtt acgagaagag gaaaagaaac
      181 gctgaatatt ttctgtacca cggattactt tcagccctgt atgggattca tattctccag
      241 cagaagcaga tggatcaagc aagagcaagt tttgaggaaa atgcacgata ctgtttgcaa
      301 agtcagtagc agtcagccct tttgttaaaa cagcattact tgtggtatca atatgtacat
      361 aaactgactc agtcatcatg ttcctcctct tatttcctct taatctcttt tattttacaa
      421 catttgcatt aaaaaagctt gtttctttta ttttaaaatt ccattacaaa atcacgtttt
      481 cattgaaaac actctcattt tcaattaatt tttgtgattt ttgtattttt ttatttaaag
      541 gaggttttat gaattgtttt cttatggaat ctacaaagtc gaatacgaga ctcatgacct
      601 ttttatgacg tactggtata cttgaagaaa aaggagagcg cgatggagat aaaaaaagtt
      661 tacttttcac agaaaatgtc gctgtattcg cctatccagc aagcaaaaca accccttcca
      721 ttttttcaaa gtcatcaaga aagtagagac gctttttggc gttctgcaaa aaagcaatgt
      781 gcttttaaag cagtatcgaa aaaatatacc gtctatttta ctattgctaa aagtagagga
      841 gatgttattt ttaataatct gttgattgaa ggtgaaactt ttgaatggaa aaaatttttt
      901 cggtatatgg aagcttgcgc tcgttttttt attaaagaaa actgttgttt tttatttcaa
      961 tctcctctat cagaagaatg gttgcgtatt tttcataaaa acggctatca aggaaccaca
     1021 caattaaata aaaagctggt ctatcataca gcacttgttt taggtggagg aggagcgcac
     1081 ggtgcttatc agattggtgt gtggcaggcg ctgaaggaac atgatatcaa ctttgagatt
     1141 atcacaggta cctcggtagg tgcgttgaac ggcgcattga ttttacaagg agatatgaaa
     1201 aaagctgtga atttatggaa aaagctgtcg acaagacaag tgcttgcgtt accagaaatg
     1261 gctgcagttg aagaccttcg tgaacgcttc tatcgagaaa gacgccaaat gacgaaaaca
     1321 gcactaattg aaggcggggt ttcttctgct ccattggaaa agatggtcaa agacaatttg
     1381 gatttgtcct tactcaaaca taatcccaaa atacgtttat atacggtgtc tactaaactg
     1441 ccagaactag cggaagtagt gactgatatc caacagacaa aaacagaaga aataccagat
     1501 tggatactcg cctctgcttc attttatccc gctatggctt atcgaaagat cggaaaaaat
     1561 aaatatgcag acggtgggta tcgcaacaaa atacctattg atattgcgat taacaaagga
     1621 gcaacagagg catttgtcgt agatgtgcaa gggccaggcc cagcgaaaaa aattcgtatg
     1681 ccagacattt ttatccattg gaaatgtcaa acattgtgga cactaggcag ctttctgctt
     1741 tttgatagcc aaaggaacca attaaattta caattaggct atctggaaat gaaaaaacga
     1801 ttaggctatt actatgggaa ctggtataca tttcattctg ttaaacctgc aagaacatac
     1861 tggcgaggat ttttgtctta cttaacaaaa gaaattcagt tagaattatc ttttttcaaa
     1921 agcataaaat tttggcaaaa actgcgtaat ttatataaga acagggtcgt accagagaca
     1981 tgcggattgg cgatgctaga acttttagca aaaaaacatt ttttactgcc caatgaaatc
     2041 tatcaagtag acatgatgat tcaaatgatc tgccaacaag atataaaaag tatgccggat
     2101 gatttattgg cacaaattgg tcaattaagt actgaagaat ggcgaaggta tcgaaaatac
     2161 cagcaaaaaa tacaaaatga gcgcacgaaa actgtttatt ttgattatct gctacaagga
     2221 aaacgcaagg atcgtttgca gcatgaattg cttaagcaac cagttgacac attactgatc
     2281 ctatatttat attatctcaa ggaggaacaa ccatggcaca agaattttca tatgaaatcg
     2341 tagaagaaat cgctatattg tcagaaaaca acaaggggtg gcgtaaagaa ctgaacctag
     2401 tcagctggaa tggtcgcccg ccaaaattcg atcttcgaga ttgggcgcca gaccatgaaa
     2461 aaatggggaa aggcctgaca ttgacgaacg aagagtttga acaactgcaa aaagcaatag
     2521 aaaatatgta atgaggaagg accctgtcga atgaaaagta tgactggttt tggcaaagcc
     2581 atccgcgaaa caaaagcgta tcagttagat attgaaataa aaagtgtcaa ccagcgtttt
     2641 ctagatgttc aaatacgaag tcctaaaatc ctgaactttg ttgaaaacga ttttcgtcag
     2701 cttattaaac agcagttaag ccgcggtcga atcgaagtat ttgtgaatct agcttatatt
     2761 ggcaaaaacg aaaaacagat cgtcatcaac tgggaattga ttgattcact tatcactcaa
     2821 ttgacagacg gaattcaaca acgatacggt agtcaaaatc aagtgtcaat tagtcggatg
     2881 gcggaacgct tcgtgctgga cgaaaacttc gtaacggtag aagaaaaacc ggaagagagc
     2941 atggaagaat tggcacagtt agctctagat acattgaaaa gcgcacttga ttctattgag
     3001 gcgagtcgag aaaaagaagg tcaagcttta gctttagtgt tgaaaaagaa tactgaagaa
     3061 ttcaaagaag tactaaaaaa actcgatagt tttgtagaaa tctatgaaaa ggaatttcaa
     3121 ataaggtatc aaaaaaagct ggaggaattt cttggagcga aagtcgatga gcaacggttg
     3181 ttgacggagc tagccatctt gttggaacgc ggagatattc acgaggaact ggatcgtctg
     3241 gcgatccatg tacaaaaact agatgaactt atcgaagcac aatgtcctgt aggtcgggaa
     3301 ttagatttcc tgatccaaga aatgaatcgg gaaatcaaca cgatcggttc caaatcaaat
     3361 gcaatcgaaa ttaaaaatca agtcgtccag tctaagacga tcttagaaaa aatcagagaa
     3421 caagtacaaa atattgaatg acgaaagagg tagagataat gaaatattat gacgtaacgt
     3481 tccacgaatt aagtggaaaa actgtggtta aacgggatat cccttctgaa aaaaacggct
     3541 ttgacgtatg gaaagatgcc tgtgcagact ataatgagaa cgaactgttt attttgatca
     3601 acgacggtgc ttacgtgacg atgaatcgga aatttattgt tcgaattgac acagaagagg
     3661 tagaagatcc gacagaaaaa gcacgttctc gaaaagatga gatcatgggt gtagtgaata
     3721 ctttgtccaa tatgggcttt tagcaaggat gctgaacaaa gaaatacaat aaataaacag
     3781 aattggattt cttcgcttag cttctttgct ctaggaatac atttaaaggt tgtgacaaaa
     3841 ttcgtgtcac aatctttttt tattttgaat aagtgatgtt caaaaaccat tttctcggta
     3901 ttaagccaaa tttatgcaaa aatgtgagaa actattttca caaaattttt cttcatactc
     3961 gaaactaatc ggcttcttca cgacctctta tacacggcgt tcaaaagctt gcatctgcga
     4021 taataacccc aaccaaacga aaaacatgag aagctgttta tgtttggctg ttcttattac
     4081 ttgatgcagg acagcttttt tacgacctct tggtacaggg tagctttttt accaatttct
     4141 agctataatt aaagataacc ttgaaagttg cagaaaaaaa gtgcaaaata aacgataagt
     4201 acaaaaagac tagttgaaag gacgtcgtca tgtcagagcg tggattatta atcgtgttgt
     4261 ctggtccatc cggtgtagga aaaggcacag tacgaaaagc aatttttgag agtgaggata
     4321 atgattttca gtattcgatc tcaatgacta cccgtaaaat gcgtgaaggg gaggtcgaag
     4381 gcgttgatta tttcttccga agcaaagaag agtttgaatc aatgatcgaa gctggagaga
     4441 tgctggaata cgcagaatat gtcgggaatt attatggaac tcctttgtca tatgtcaaca
     4501 agacattgga tgaaggaaaa gatgttttct tggagattga agtccaagga gcaaaacaag
     4561 tcaaagaaaa agtgcccgat ggtgtattca tctttttgac tccgccagat ttagcggaat
     4621 taaaatcacg gatcgtaggc cgaggaacag atgcagatga agtaatcgaa aaacgaatga
     4681 aagttgcaaa agaagaaatc gaaatgatgg ctctttacga ctatgctgtt gtaaatgatg
     4741 aagtgccgct agcagttgaa cgaatcaaaa acattattgc cagtgaacat tttcgagtag
     4801 atcgcgtaat cggtaaatat attaaaatgt taaaggagat gtagtcttat gatgttaaaa
     4861 ccttcaattg actcactatt agatcgtgta aattcaaaat attctctagt gattttagca
     4921 agtaaacgcg cgcatgaatt ggatgcaagt gcacagccta ctctagattc ttttgattca
     4981 gtaaaaagcg tcggtcaagc tttagaagaa atcgatgcag gaaatgtagt caatgatcca
     5041 catcctgaat taaaacgtga acgtttgaag atggaagaag aggaacgtca agcacaaaaa
     5101 gagcgtgaac aacacgaatt ggaatcacgt atccgtgaag agcaaaaaat gtaataaaga
     5161 aaaatagagc gaacatctaa aaagaaaagc aaacccagct gtttcctttt caaacattcg
     5221 ccacttattg tctggtagaa aaggctgtgt ctgaactttt tgacacagtt cttttttttc
     5281 ttatctgctg ttaatttttt gttaggattt ggcagaagat aagacaaatc catcgatctc
     5341 tagtaaaata ggcaaggaac tttttgtcgc ttgataagaa aagacaaaaa aaggaggaaa
     5401 cgaaccatga cattaacagc tgaagtcatt gtggacgtac ctactatgca gaccgatcag
     5461 ccattcactt atttggttcc ctctgaaatg gaaacagctt tgcaagtcgg gatgcgagta
     5521 gaagtccctt ttggtaatgg caatcgtcat gtccaaggat ttgttatggc tatccaaaaa
     5581 tcagaagaat cagctaatcc atctttgaag gcagttattc gtttgctaga ccttgctccg
     5641 gtggtcaatg aagaattact ttcattagct gattatatga aaaaaattac ttacgcattc
     5701 aaaatcactt gtctacaaac gatgcttccg agtgtgatga aagcagaata tgataaattg
     5761 atctatcctt tggcagacac accagaagtt caggtattgt ttgatcagcg agaagtgatt
     5821 tcctggaagg aagcagaaga agaaggcagc ctttctcaat tgattcgctg gcgccaagag
     5881 caattagtag atatcaaata tgaagtccat acacgaaata aagtaaaaac gattcgtttg
     5941 gtccgttctt tgttaactga aaagcaaatc gaagaagaat gggcaaaatt acggcaaaat
     6001 gccaagaaac aaaaagaact tcttttgtgt ttatctgaac tgagccaaga agaaccaatt
     6061 gcttatttca aaaataaagg aatcagtact gctgtcttga atcaaggaaa agaaaagggc
     6121 tggctggagt ttatagaatc agaacgttat cgcgaccctt ataaagaccg agtatttgac
     6181 caaacgacgg cattggaact gaatgcagaa caaaaaaatg ctgtcgaaca aatcatcaca
     6241 gcaggtcagc agcaaaaaga tcaggtcttt ttgctggagg gaatcactgg cagcgggaaa
     6301 acagaagtat atcttcaggc aatcgctgat gttttgtcag aaaataaaac ggctattatg
     6361 ttagtgcctg aaatcgcatt gactccgcaa atggtcgagc gattcaaagg acgcttcgga
     6421 gaatcagttg cggtcttaca tagcggactt tcccaaggtg aaaaatatga tgaatggcgc
     6481 aagattgagc gggaagaagc gcaagtagta gtaggcgcac gatctgctat ttttgccccg
     6541 ctgaaaaata tcgggctgat cattatcgat gaagaacatg aatcaagtta caaacaagat
     6601 gagacaccgc gttatcatgc gagagatcta gcaatttggc gcagtaaata tcatcattgc
     6661 ccaatagttc taggaagcgc gacgccctct ttagaatcaa gagcacgagc tcaaaaaggc
     6721 gtttatcagt tattgcagtt aaaccaccga gccaaagcag ctgcacagct tccagctatt
     6781 gagatcgtag atatgcgtga agagtttcag aatcatcgga cctctacttt ttctgctaat
     6841 ttgcaggaaa aaatacaaaa tcgattagac aaaaaagaac aaactgtcct actgctcaat
     6901 cgtcgaggat attcgtcttt tgtgatgtgt agggactgtg gatttgttct tccatgtcct
     6961 aattgcgata tctctcttac actgcatatg gatacacgtt caatgcgctg ccattattgc
     7021 ggacatgaag aacctattcc caatcgctgt cctaattgtg gcggcaataa aatccgctac
     7081 tatggaacag gtactcaaaa agtagaagag gaactccgag aactttatcc gcaagctagg
     7141 attttgcgta tggatgtaga taccactcga agaaaagggg cacacgaaca gattttacaa
     7201 aaattcggtg caaaagaagc agatatatta ttagggacac aaatgattgc taaaggtttg
     7261 gactttccag aagtgacatt agttggggtg ttgaatgctg atacctccct gaatttaccg
     7321 gattttcgtt ctagtgagca tacattccaa ttgcttaccc aagtttcagg acgagcaggg
     7381 cgagcggaaa aagcaggcga agtcgtgatc caaacattca atcctcaaca ttacgccata
     7441 gaattggcta aaaaacaaaa ttatgagcag ttttatcagc aggaaatgca tgtacggcat
     7501 cgtggaggct atcctcctta ttactttact gtgaaaataa ccgccagcca tcctgaagaa
     7561 caagtagcgg caaagaaaat ctttcagatt gccaatcagc taaaagaagt gttaagccct
     7621 caaagcttgc tattagggcc aacgcccagc atgattttga gagtgaaaaa ccgatattat
     7681 tatcaactaa tcatcaaata taaacatgaa ccgaatcttc ctcatgtttt ggatgaaatt
     7741 ttgaatggaa gccagaaaga acagcgacaa ggtctttttg tggcaattga caacgaacca
     7801 ttaaacttta tttaagaaag ggccaaatta tgcgctatcc tatattgatc caccctaatg
     7861 ataaattaaa aagaactgct cagccaatcg atgtaatcac ggacgagaca atcgctttat
     7921 tagacaattt atatgagaca atgattgcga atgacggaat agggatcgct gctcctcaag
     7981 taggacaaaa caagagaatt gctgtgatcg aagtagatga aggcgagaaa tttgaactga
     8041 ttaacccaga aatcatcgaa gcaaaaggtg aaagccttga cgtagaaggt tgtctcagta
     8101 ttccgcatgt ctatggaacg gtcaagcgag cagatgaagt gaccgtgcgt tattatgatc
     8161 gagacggaga agagatagaa gtgacggctt ttggctattt agcccgtgcc ttccagcatg
     8221 aaatcgatca cttagacggt atcttgttca ttgagaaaat gatacagcag atcccggaag
     8281 aagaacttga agaatatatg gaggaacatc ttgatgacta agctagtatt tatgggcaca
     8341 cctgcttttt ctgtgccgat tcttgaaggg ctgatagaat caggttacga gatccaggca
     8401 gtggtaacac agcccgaccg tccagttggg cggaaacgag taattacgcc gacaccggta
     8461 aaaaaagcgg ctctgaagca cggtatccgt gtgctgcagc cagagaaaat ctccggttcg
     8521 ccagaaatgg aagaaatcat tgaattggtg cctgatgtga tcgtcactgc ggcatttggc
     8581 caattcttgc ctgaaaagtt attgcaagta ccaaaattag gggcaatcaa tgtccatgct
     8641 tctttacttc ctaaatatcg gggaggtgcg cctgttcatt atgccatcat gaatggggag
     8701 aaagaaactg gtgtaacgat catggaaatg atcaaaaaaa tggatgcagg cggaatctat
     8761 gcgcaagaaa gcataccaat cactaaacaa gatgatgtag ggacgatgtt tgaaaagctt
     8821 agccttttag gaagaaagct attgctagaa acattgccca atattttgga tggacaaaaa
     8881 ccagtacctc aagacgagtc agaagcaact ttttcgccaa acatcacacg ggaacaagaa
     8941 gcaatcgatt ggcataaaac tgcagaagaa attgacaacc aagtacgcgg aatgcgtcct
     9001 tggccaattg cattcacgac ctatgagggc atacgctgga aattattatc ggttgaagta
     9061 ttggcagaaa ctacagacaa agcacctggt acgatcatcc ggaaagacaa aaaaaatcta
     9121 tggattgctt gcggtaaaca gaccgtctta gccatcaatg aattacagcc agctggtaaa
     9181 ggaaaacagg ccgtacatga tttccttaac ggaagcggtc agcaagttca gattggtcag
     9241 caggtgaaat aatgaaaaaa atccctaaaa aaatgaaaaa atcagtacga tatatggcat
     9301 tactgacatt agagcgagtg aacaagggtg gcgcctactc gaatcttttg ttgaatgaga
     9361 tgatcaaaca aagcgagcta aatcaaaaag atgtgggttt atttacagaa ttgatctatg
     9421 gtacgattag ccatcagcga ctattggaat tttacgttgc accattgatc gctaaagcaa
     9481 aaaaagtgga tgactgggtc aagatgcttc tttatctttc tgtataccaa ttagaatttt
     9541 tagataaagt ccctgcacat gctattttaa acgaggcggt cgaaatcgca aaagtaaaag
     9601 gaaatcctgg gacagggaaa tttgtaaatg gtgtcttgag gaattatcag cgtcaaggag
     9661 caccagatct gcaaggcatt gctgatccta tcgaaagact ttctgtcaaa atcagcctgc
     9721 ctctatggat gacgaaacga ctggtggatc aaatcggata cgaagaaact gaaaagctcg
     9781 gtttatcgct atatcagcct agccatgcta gtgcccgagt agatacacgc cgactttcca
     9841 gagatcaggc catcactgtt ttgcaagaag aagagatcac agctgaaaaa agccaagtca
     9901 gtccatacgg tatagtagcg aaaaaagggc atttagcttc tagctcattg ttccacgatg
     9961 gcgtcatgac gatccaagat gaaagttcga tgcttgtagc gccgtctatg caaatcgagc
    10021 ctgaacatca tgtcttagat gcctgtgcag cacccggagg taagacgacg catattgctt
    10081 cttttttaga tccgtcatgc ggtggagaag tggtcgcttt agatgtacac gaacacaaag
    10141 tcaaactcat tgaagataat gcagcacgtc taggtgtctc ttctgctgtc tacgctcaaa
    10201 agatggatgc tagagaagtc agtaaacagt tcgaagcaga gcagtttgat cggattttag
    10261 tagacgcgcc ttgttcaggt cttgggctgc ttcggcgaaa accagatatt cgttataaaa
    10321 aacagccgga tgaattagcg aatttaccaa aaatccaatt ggaaatttta gaaagctgcg
    10381 caccaacttt aaagtcttcc ggtattctga cttatagcac ttgtacgatc ttaaaagaag
    10441 aaaaccaaga cgttgtttct gctttcttgg aaagacaccc tgattttgag aggattgatg
    10501 ttttgaccaa tcctatgcta caatcatcaa tcgaagagaa aatgttgact ttgtatccgc
    10561 atcagtttta cacagatgga tttttcattt gttgtttgcg caaaaagtaa tgaggtgaaa
    10621 aaatgcagat tgaatatcaa tcagatgtag gaagacgacg taacacaaat caagactatg
    10681 ccagtgtatt taccagccgt tcaggtctca agcttgctgt attagcagac gggatgggtg
    10741 gtcatcgtgc cggagacatt gccagtcaga tggcggtaac gaatctaggc gaagcttggg
    10801 aaaaacaaga agctgttgac gatgaaaaaa tcgcccaatg gttcattcaa tcgattcaag
    10861 aagaaaatat gctgatttat cagcgtggac aagaacagcc ggaatataac ggtatgggaa
    10921 caacaatcgt agcggccgct ctgtcagatg actggttcac gattgccaat gttggggata
    10981 gcagagctta tttgattcgt gaagataaaa tcatgcaatt gacggaggat cattctttag
    11041 tcaatgaact agtcaaatca ggagaaatca gcgaagaaat ggcagtgaac catccaagga
    11101 aaaatatttt aacacgttct gtcggcatgc ctggaacagt cgaagtagat gtttctactc
    11161 atctttggca actaaaagat cgactgctgt tatgttcaga tggcttgacg aacatgctgt
    11221 ctgaagaaga aataggaacg atcatcaatc aagaaggccc attgtctcat aaagtaactg
    11281 agctgattga tcgtgccaat gaagcaggcg gagcggataa tatcaccgtt cttcttatcg
    11341 aatataagga ggacagtgta tgatcgagtt gggcaaaaaa ttaaatggcc gttatcaaat
    11401 cactggaaat atcggtagcg gtggtatggc aaatgtattt ttggcgcacg atttaatctt
    11461 ggatcgtgat gtagcagtta aagtcttacg atttgacttt caaaatgacc agactgcgat
    11521 tcgacgattt cagagagaag ctttagcagc aacagaactc gttcatccaa acattgttag
    11581 tgtttacgat gttggtgaag aagacaacat gcagtatctg gtgatggaat acgtcaaagg
    11641 aatggacttg aaacgatata tccaaacaca ttatcctgtt ccttatgaaa cagcagtaaa
    11701 tattatgcag cagatccttt ctgctatttc tttagctcat aaccatcaaa tcatccaccg
    11761 agatttgaaa ccccaaaacg tactgatcga taatgaaggt gttgtaaaaa tcacggattt
    11821 cgggattgct atcgctttat ctgaaacatc gatcacgcag acgaatacga tgctaggatc
    11881 tgttcattat ctgtcccctg agcaggccag aggaagtatg gcaacgaagc agtcagatat
    11941 ttatgcttta gggatcatcc tttatgagat gttgacgggt agcgtaccgt ttgatggtga
    12001 atctgctgta acaattgctt taaaacattt tcaagatgat cttccatcta taaaagcact
    12061 tgatccaaat gtgccacaag cactggaaaa cgtgatttta cgggcaacgg ctaaagaacc
    12121 tgcagaccgt tataagtcag ccgaggaaat gtctgatgat ctttctacag tattatcacc
    12181 agctcgtgca aacgaagaaa aatggcagcc tcatgtgatg gataatgaaa caaaagttat
    12241 cacacccctt acggaagatg caccaatgcc tgattcattc aaatctatgc ctctgccaaa
    12301 agacaagcca gaaaacgaaa ccttaccaga ggaggaagaa gttctgcctg atcctaaaaa
    12361 gaaaggacag aaaaaatggt ggctgatcct attagctatt cttgctgttt taggaatcgg
    12421 aacagctgta ttctttgctt ctggcgggcg aggagaagtt gttgttcctg atgtttctga
    12481 tctttctgaa gcttctgcta gagaaacact cgcaaaagcg aaattagaag tagcaacaaa
    12541 aacagaagaa attgcagatg acaaaatcga agaaggtaat gtagtgaaga cagacccggc
    12601 agcgggaacg acagtcaagc aaaaacgcga agtcactctg tatattagta gcggcacgaa
    12661 aaaaatcaaa ttagatgatt acagtggtaa aagttatgaa gatgccgaag caagtttgaa
    12721 agaattaggg ttttctgctg acttgattca aaaagaagag gaattcagtt cagaggtaga
    12781 aagcggaatg atcatttcgc aatctccatc agaagataca gaagtcgatc ctaaaacaga
    12841 cacgattact ttcacagtca gcaaaggacc agaaacagtg actttgagta gtcatgtagg
    12901 agaaaactac gaaactgttc gtaattggct tgttacacaa ggattcaata tcaataatat
    12961 tagtgctact tatgactata gcgacactgt accatcaggt tcgatcatca gtcaggatcc
    13021 agcctcgggt gaagttgtag cagaagaaac ttacatcaat tttgtcgtaa gccaaggaac
    13081 agagcctaaa ttgaatgata tctccggcta tacaaaaagc gaagcacaaa gttatttagc
    13141 tagtgtcggt gcagaatata tcgggcatga gacttccgaa tattcaaaca cggtggataa
    13201 agataaagtg attcgtacag taccaggggc aggaacgaca attaccaaag gaatggttgt
    13261 gaacgtaatt tactctaaag gaccagatcc agcgtcttct agttctgaaa aagactcttc
    13321 agacagttct agcagctcaa gtagctccag tagcacaagc aactcttcaa aagaagaatc
    13381 ttccagctcc acaagttcat cagctagcac ctcagaaagt tcaaaataag tgctgaataa
    13441 aatatatcag aagggaatgc gacattgatt tgttgcattc tcttttttgt gtttctatct
    13501 cttttgatca attttcaagc catatacttt gagaagtttg catggaataa cacctacttt
    13561 cctcttcgta tggtaaaata aacaaaaacg gaggtggcac ttatcaaagg acaaatcaga
    13621 aaagcaatca gcggttttta ctatgtatac gcaaacggag agacctatca aacaagagga
    13681 agaggaaatt tccggaatcg gaaaatcacg ccgcttgtcg gagatcaggt gatctttgag
    13741 agtgaaaatc aaacagacgg ctatcttctg gaaatcttgc cgagaaaaaa tcaattagtt
    13801 cgcccaccag tcacaaatgt tgatcttgga atcattgtca caagtatcgt agagcctgca
    13861 ttttcttata atctacttga ccgctttcta gttaccttgg aatacgagtc gatcgaaccg
    13921 attatttttt tgacaaagac cgacttgtca gacgacccag aaaaaatcga agagatcaag
    13981 caaacctata ctaaaatagg ctatccggtg attgtaccca atcatacggg tgatacgaca
    14041 gaactgatcc gttacttccc agaacggcta tcggtcttta tgggtcagtc tggagcagga
    14101 aaatcgacgc tactgaatca aatatcaccg gaactgaatt tagcaacagg agagatttct
    14161 gatgcacttg gaagaggacg gcatacaaca cgtcatgtgg aactgattcc tatctatggc
    14221 gggttagtag cagatacacc aggatttagc tcgattgact ttttgacaat tgaagcagga
    14281 gaactgccga gacaatttcc tgaatttgta gaagcttctg tccattgcaa atttagagag
    14341 tgcatgcatg ctaaagaacc tggctgcgaa gtaaaacata gagtagaaac aggtggtatc
    14401 gcacagacac gttacgacaa ttatttgcaa ttcttacaag aaatcgaaaa aagaaaacca
    14461 atgtatgcaa aaaaagaagg taaacgaaaa taggaggaaa caagaatgaa gatagcacca
    14521 tccattttga gtgcagattt tgccaattta caaagagaca ttgagttagt ggaaaaagga
    14581 ggcgcagatt acatccacgt agatgtaatg gatggccgat ttgtcccaaa tatcacatta
    14641 gggccaaata ttgttcaggc aatccgtcct gttactaaat taccgttaga cgtccatctg
    14701 atgatcgttg atccagaaaa ctacattcca gcatttgcaa aagctggagc agatatcatt
    14761 actgtccacg tcgaagcaac tcctcatatt catcgggcac ttcaaatgac aaaaagctta
    14821 ggagttaaag ctggagtagt tatcaatcca ggaacaccag tctccatgat caaacatgtt
    14881 ttgcctttgg ctgatcaagt actagtcatg actgtcaatc ctgggttcgg cggacaaagc
    14941 tttattgaag aaacagtaga aaaaattacg gaactcgctg atctacgtag agagaacaat
    15001 tggacttatg caatcgaagt agatggcgga atcgttccag aaacagcaaa aatctgtaaa
    15061 gatgcagggg cggatgtctt cgttgcagga tcatatatct atggagccga aaatccagtc
    15121 ggacaaatcg aacgattaaa agaggcatta gactaaatga aaattttatt agctgctgga
    15181 ggtaatccgc aaaactggcc gcagtctctg ctgtccaaat acgatgccta tgtcgggata
    15241 gaccgtggtg cgctgtacct gcttcaagca ggctacccag tagatctggc agtgggtgat
    15301 tttgattcat tgtcgaacga agagcagaag gaagtatttg caaaagccaa agagtatacc
    15361 cgttcccctg cagaaaaaga cgatacagat acacagttag gtatcttaaa aacatttgaa
    15421 cgttacccgc aagcggaagt cactttgatt ggagctacgg gcggaagatt ggatcatctt
    15481 ttagccaatc tctggttagg tatggaacct cgcttcaaac cctttgccca tcagcttatt
    15541 atcagagatt gtcaaaatta tttgtcttat tatttgcctg gagagcatat agtaagacag
    15601 ataccagata tgaagtattt agcttattgc tgcttgactc ccgtctcgaa tcttacatta
    15661 aaaggcagca agtatgtatt ggaaaataaa gaagtagaat atccgacatc ttatgcaagt
    15721 aacgaatttc tttcagatac cgcagaattt tctttttcag atggtatgat tgcagttatt
    15781 caaagcaaag attgatccat aaacaaataa aaaaatttgt ctttcaggct aaaaatgaca
    15841 gaatctatta tttgctaaaa aaaagcttaa gaggagcaac ttaccttttg tttcacggca
    15901 tttagtgata aaattgaaca caagagtcta attaattcga ggtgtctttg tgaagaagtt
    15961 caatcctaat aaaaatatta ttattacctt ggttattgtt atcatcgtgg tgacgatact
    16021 aagtgtgacc gctgcgcagc gagcgaatga aggaaagccc aatttcttcc aatccatcgt
    16081 taatgacagt gttgcaatgg ttgataaaac aatctctgcc ccagtacggt gggtacgaaa
    16141 tggagtagat tctgtccata atcttttttc aacttatgcc gaaaatgagc ggctaaaaga
    16201 aaaaatcgat agctatgatg acgtttcaca gcaaaacaag aattacgaga gagaaatcga
    16261 agcgttgaaa aaagaacttg agttgaatca gacgctgact agttacgaaa aagtaacggc
    16321 aaatgtcatt actcgttcgc ctgacagttg gcaggacatg ctgatcgtag acaagggttc
    16381 taaagacggt atcgaagcaa atatggcagt catgtcgcaa aaaggattga ttggccgagt
    16441 gatcgaggtc aatacggctt cgtctaaaat cgaattactg tcatcctcta atgaaagctc
    16501 caatcatttt ccagtacggg tatcttcggc taatggcgaa gcgtttggtt tgcttaaaaa
    16561 ctatgatgaa aagctccatg ccttagtggt gacccaatta actggtgata cggatatcaa
    16621 agaaggggat gttgtccaga catccggtct tggagggaat tctccagcta acttgccgat
    16681 cggtacggtt attaaaacga aaccagatag ttatgggctg gatcgggaag tttatgtgaa
    16741 accttatgca gaaatgtatg acgtgtcagt tgtgacgatt gtccaacggc tagcggagga
    16801 ttagtccatg ttgaagaaag aaacaatgaa gtactatctg ccaatcgttt tgttcttttt
    16861 gatgttgata gatggtcatt taacaagaat gctaggggag tggtcgaaag gcacctatat
    16921 gtcaaatgcc cactttctga tattggcatt attatgttgc agtatggcgt ttgaaaaacg
    16981 ttatttactg attaccacga ttgttctcgg ggctatctat gacgcttact atattggcgt
    17041 tatcggtatc tatgcagtag ctctcccttt aattgtatgg ttgatgtatg taatgaaaga
    17101 cgttatccat gtcaacatct ttactgaatt tttcagtatg atcatctttg tcacgggtta
    17161 tgaattgttt acgatggtgg tccagttgat ttttaaatta gcagtagtaa ataacacgta
    17221 ttttattaca aggtttttag gacctacact gctgttgaac atgattatat ttgtattatt
    17281 catttttccc tttaagaaat tattcagtga tgaataattt ctttttttat ttttaggtcg
    17341 aaagcgaaaa agaatcgttg ttatcttaag aaatgctaga cttaacgcgg ctttttcatg
    17401 atcattttct gaaacgaaca gttttgtcat ttttaaaagt tgttttgaaa catttttgta
    17461 acataactat tatatcgttg acacactgct atggtacaat ttttatatcg ttaagaaaga
    17521 cttaaatata tttagatatt tattaaatta agccagtcgg aggaatgaag agtgaaaaag
    17581 agtttaatat cagcagtaat ggtatgttca atgaccttga ctgccgtagc atcaccgatc
    17641 gctgccgcgg cggacgattt tgattctcag atacaacagc aagatcaaaa gattgcagac
    17701 ttgaaaaatc aacaagcaga tgcacaatca caaatcgatg ccttagaaag ccaagtttct
    17761 gaaatcaaca cacaagcaca agacctatta gcaaaacaag atacattgcg tcaagaatct
    17821 gcacaattag taaaagacat tgcagattta caagaacgta ttgaaaaacg tgaagataca
    17881 atccaaaaac aagcacgcga agcacaagta agcaacacaa gctcaaacta catcgatgct
    17941 gtattgaatg ctgattcatt agcagatgct attggacgcg ttcaagcaat gacaacgatg
    18001 gtaaaagcaa acaacgactt gatggaacaa caaaaacaag ataaaaaagc tgtagaagac
    18061 aaaaaagctg aaaacgatgc taaactaaaa gaattagctg aaaaccaagc tgctttggaa
    18121 tctcaaaaag gcgatctttt atctaaacaa gctgatttga acgtactgaa aacttcatta
    18181 gctgctgaac aagcaactgc tgaagacaag aaagctgact tgaaccgtca aaaagcagaa
    18241 gctgaagcag agcaagctcg tattcgtgaa caacaacgtc tagcagaaca agctcgtcaa
    18301 caagctgcac aagaaaaagc tgaaaaagaa gcacgcgaac aagcagaggc tgaagcacaa
    18361 gctactcaag cttcatctac tgcacaaagc tcagcaacag aagaatcatc agctacacaa
    18421 tcttcaatga ctgaagaatc atcatcagct acacaatctt cagcaacaga agaatcaaca
    18481 acacctgaat cttcaacaga agaatcaaca gcgcctgaat cttcagcaac agaagaatca
    18541 acaacagcgc ctgaatcttc agcaacagaa gaatcaacaa cagtgcctga atcttcagca
    18601 acagaagaat caacaacagt gcctgaatct tcaacaacag aagaatcaac aacacctgcg
    18661 cctacaacac catcaacaga tcaaagtgtt gatacaggaa acggcacagg aagtagtact
    18721 ccggctccaa cgccaacacc aacacctgaa caaccaaaac ctgtaacacc tgctccagct
    18781 ccttctggaa gtgtgaatgg tgcagcaatc gtagcagaag cttacaaata tattggtact
    18841 ccatatgttt ggggcggtaa agatccaagt ggctttgact gctcagggtt cacacgctat
    18901 gtttacatgc aagtaactgg tcgtgacatc ggtggttgga cagttcctca agaatcagct
    18961 ggaacaaaaa tttcagtttc tcaagcaaaa gctggagact tgttattctg ggggtcacaa
    19021 ggcggaactt accacgtagc gattgcttta ggcggaggac aatatatcca tgctcctcaa
    19081 ccaggcgaaa gcgtaaaagt tggttcagtt caatggtttg cccctgactt tgctgtcagc
    19141 atgtaagaat gattaatcaa aaagagttag cccattctgg actaactctt tttttattgt
    19201 gataaaatag aaatcagata aattcttctg tcaattccat gaacgtttgg atatcttcag
    19261 tcaccatgtc gatacctgct tgccaaaaat ctggttgggt aagatcgaca ttcaaatgtt
    19321 tcttcgcaag atcttccgtc gtcattgagg cagtatcgcg tagtaaatgg atatattctt
    19381 cttcaaaatt tgctcccttt tggttggcgt aagcatagat gcccaaacta aagaggtagc
    19441 caaatgtgta tgggaagtta taaaacggta cgtcatcaat aaagaaatgc aattttgcag
    19501 cccagaaata tggatggtaa gtagctaaag ctccttgata gccttctttt tgcgcctcaa
    19561 ccatcatctc tgtgatctta tcttctgaga ctaatccttc ttgccgtgct tcatagaatc
    19621 gattttcaaa aatgaatcgg gaatggatat tcatgaacat agctaaagca ttttgcagtt
    19681 ttgtatctag caaattgatt ttttcttctt ttgtttttgc gacttttaat gtagcatcgg
    19741 ctacgatcag ttctgcgaat gtacttgctg tttctgcgac attcatggca tattcgcgat
    19801 ttaaagaagg taaatcccac atcgtgctgc tatggaaagc atgtcctagt tcatgagcaa
    19861 gtgtcgcaac ctcattaaca gaattactgt aggtcataaa gatccttgat tcttttgttt
    19921 ctggtaactc agtacaataa ccaccaggac gtttcccagg acgatcttct gcttcgatcc
    19981 agctcttttc aaaagcagat tgggcaaatt tagccatttt tggactgaat ttgttaaagt
    20041 tttcgataat aaatgctgct gcttcgtcaa aactaaaggt tttttctttc aaatcgccta
    20101 aaataatagg tgcatcttga tcttgccatt ccattttttc tttaccgaaa agattagctt
    20161 ttctagtcaa gtaattaaca aggggttgtt tgtttttctg gattgtatcc cacatgacag
    20221 acaatgtttc tttcttcaac cgattgtact ctaatggttt ttgtagaaaa tctgtgacac
    20281 catgaagttc atagtcagaa agtctaaaac catctaaatg attcaaagta tccgtgaaga
    20341 ggggtgcctt ttctttccag gcttcttccc atgctgcgaa aagtgtttct ctgacttttg
    20401 gatctggatc gcccatcatc ttattgaagg cttgtccagc agataattcg actacttgtc
    20461 catcttgttc gaagggaatc gaaatgctgg ctacaatcgt atcataatga ctgctccaag
    20521 catttagacc atctaaagaa agcgtattta taatgttttc ttcagcttct gataataatt
    20581 gtgagccatc acgacgaatc tcgtttaaac gaaaagcaat tgtttcaaac gaagattgag
    20641 aaagcatctg attccaatca ttgtcagata tttctgcaaa tttctttgac aatacagttt
    20701 cggctgattg cagacgaggc agcattgcgt aaagcttgcc tgataagacc tttgcatctg
    20761 aatcattgac attggcagaa agcaatgcag taatatagct gctgcattgg gtaaaaccat
    20821 tcgtaatcgt ttcttgcaat tgaagaatag cgtttaactg ctctgcattg tcagaactag
    20881 gtgaccattt tgttacgcgg tgataatact cgttcatttg gtcttccaat tgcttcatcc
    20941 gttggttcaa agcatcagaa tgactgcctc cggaaaaaat agaatctaga ttccaattta
    21001 atgaataact catgaaaaaa ctcccttcat attgtaatac aaaattaagt atagcacttt
    21061 tttatggaag aagtctctat gaaaatgatt gttgtttctt caaaaaaagt ctaagataaa
    21121 atacagaggt gacttatgaa ttcttcaaaa aaacaaatga aaaacggaaa tatttattta
    21181 gtaatcgcac tgatagtcgt tataatctta ttttacagtt cttctcaaac ttacggacag
    21241 caatcacaag tttcgcgatt agaaagctgg ctgcctaatc agccatttaa agacacatta
    21301 gcgggtatac gttttaatta tggtggaagt atggtgagta tcaaccatct gggctacgcc
    21361 aaatttttag agttcttttt aaggaagggc gcacatttcg gtacctattt tattttggga
    21421 ggcagcttgt ttttaggtgt tttccccaag ctaaaaattt ggtggctgac agcgatcctt
    21481 gcttggcttt cagcaacagg gtacgcaggt ttggacgaat tccatcagat gatgaccggt
    21541 ggacgaacac ctatgttcca agatgtcatg ttggattcga tcggtgctct gactgctgtg
    21601 atattatgtg tgctttttgc ggcattcaag aagcgtttat agattggctg tggctttgtt
    21661 tttttgtgaa atagtactag aatagaagtg tattctcaaa agaacaaggg aggaaaaatc
    21721 atgtcaggac atagtaaatg gcataatatc caaggaagaa aaaatgccca agatgcaaaa
    21781 cgagggaaag tctttcagaa actttctcgt gaaatttata tggcagctaa agcaggcggt
    21841 cctgatccat ctatgaatcc agctttgcgg cttgttgtgg acaaagcaaa agctgcaaat
    21901 atgcctaacg acaatgtgga acgggcgatt aaaaaggcta cgagtgccgt agatgaaacg
    21961 aattacgatg aaatcacgta tgaaggatat ggtccgggtg gagtaggaat cttagtccat
    22021 gcattgacag ataaccgtaa tcggacagcg acaaatgtcc gagtagcttt tactcgaaac
    22081 ggtggatcac tgggagaaac gggttcagtc agttacttgt ttgaccgcaa aggatatatc
    22141 gctattgaac gcgatgggtt gactgttgat gaagagacga tgtttgagga tgtattagag
    22201 gcgggggcag aagatctgca aacttctcct gaagtattcg agatttatac tgcaccagaa
    22261 gattttgctg ctgtacgaga tgagttggaa aaagagttca ctttggcgca agcagaattg
    22321 acgatgattc cacaaacgac gattgattta aatgaggaac aaaaagagca attggaacgt
    22381 ctagtagata agctagaaga cgatgatgat gtttcagaag tctttactgc tgctgaaatg
    22441 taagtagcac attgttagat aataaaaaga aagcacctgt accaatcgta cgggtgcttt
    22501 ctttttatat attatttttg ttagaagacc aatacaggcg ttcctttttc gaccatcccg
    22561 aataattcct tcattacact tggtggggtg ttgatgcagc cgtgtgaacc gcgggttttc
    22621 cataagtctc caccgtattc aggttgccag tctgaatcgt ggataccaac acctgtccag
    22681 tcaatcggca tccagtagtt taccggactc tcgtaaggtg ttccatcgtc atttgttcct
    22741 tttaaagttg catcttcttc tttattccaa acatagaaga ctccagcagg tgtaggtgta
    22801 gttggctttc cggaaacgat atctgtttca agggccactt ttccgtcttt gtaataccac
    22861 atatgctggt tctccagatc aacttcgata tacgtatctt cgatcaatgg atgatcagcg
    22921 gttgtgccac cttgcacaat gggtgaacga gtaaaatctt ggcctgccag aatcgctttt
    22981 ttcaatgctt ctgtctcact gtctgtttgg atcgtccaac tgtaagtgcc aactgggacc
    23041 gtaacttctc ctctttttgt acttttaaat tttgtgtcat tcgtacttgt gttatacttt
    23101 gtgcctaaat ctgttacata ttgacgtact tgttctgtat ctaaatcgac ttttccatca
    23161 ttgtaggtca gccaactcat aatatcactt gatgggatct ggaaagtctc accgtttata
    23221 ctgtaagtag cttttacatt tgcgatagca ttcatggatg cgagttgttc tttcagtgag
    23281 ctgtcttcag atgttacttt tggtttttct ttgaattcag tcaattcaat tgtatcttta
    23341 ccagagttta ccgcactttt taattgtttg accgcagcat cgacgtcgat cgtatttcca
    23401 ttgacttcag gcttgataac gaaagagtca cctgcttttt cgatcgttgc atcagtagta
    23461 ggtgtccggt ctttgttcaa atcagtcagc ttagttgtca atgtctgaac ggtagtgtct
    23521 agtttttggt ggttttgact gacaggatct atttcttgtt tttctgccgc tgagacataa
    23581 gtcattcccc aagtccatcc gttttgttca tttaggatcg gagaaagctc cgaagtgaaa
    23641 tcatctttgt agccgagctc ggattttggt acttcctgcc agacttttcc atcttcttta
    23701 atagccagtt ttttatcaga ataagcttct tttaattttt catttgcttt ttcgactgtt
    23761 agattgctga catttattcc atttatttct gtattcggca aaaaatgctt tgtgtagtat
    23821 gtactgcgat aggtataaaa gctgatcagc actaagagaa caccaagaac tgaaagcaaa
    23881 gcaacatgtt tcgatttttt tcgatgcgaa gatcttgtca tatcgacaac tccttaagta
    23941 aaagtttagg gtggaacggt ctcatgatag cataatttag aggaaaagac gaaggttatc
    24001 tctactttat gaaaaaattt agaaaccata agggtgataa aataaaaaaa cggaaaacac
    24061 cgaatagatg ttttccgtca tgttcaaaga ttattcagcg tctagtttag caaaagcttc
    24121 atctagaatt tcttttaatt gcttagcaga agcagccata cggtcttgct cgctgtcact
    24181 taaaggaatt tcgattactt tttggatacc ttgacggttg ataacagcag gtgcaccaat
    24241 gtagatgtcg ttcaatccat attcgccatt catgaatact gataaaggaa gaacagcatt
    24301 ttcatcatct aagatggcac gagtgatacg tgctaatgct actgcgatac catagaaagt
    24361 agcgcctttt ttctcgataa ttgtgtaagc agcgtcacgt acgccaaaga ataggttaac
    24421 catagcttct tcatcgattt ctgggttgtt ttttacccat tcatagattt gcaagccggc
    24481 aacatttgca tgtgaccaaa ctgggaattc tgaatctccg tgttcaccta agatgtaagc
    24541 atggacgtta cgtgcatcaa cgtcaactaa ttccgcaagt gcttgacgga aacgagctga
    24601 gtcaagagag gtacctgaac cgattacgcg ttctttaggg aaacctgaga atttccaagt
    24661 tgagtaagtc aaaatatcta ctgggttagc ggcaactaaa aagataccgt taaatcctga
    24721 ttcaacgatt tgtgtgacaa catcgcggtt gatttttagg tttttgttta ctaagtctaa
    24781 acgagtttca cctggttttt gtggtgcgcc agctgtaata acaactaagt cagcatcatg
    24841 tgcgtctgcg taagtagcag aatagatttt tttaggtgaa gtaaatgcca aagcgtgaga
    24901 caagtcaatt gcgtctcctt cagttttagc agtattgata tccacaatac ctacttcttg
    24961 agcgatattt tgagtaacta gagcaaaggc atagctagaa cctacggcgc cgtctccaac
    25021 aagaataact ttttgatgat cttttttttc tgcagctgca gtcatttagt atcaatcctt
    25081 ccattgattt gttttttccc atgcttatgc taacatttct caagccccat gtcacgctat
    25141 ttggctataa aatataacag ataacaaggt tgtattatat gtaaaagctt acatgtgcgt
    25201 tatttcacta tttttatata tcaattattt cattaaactt tgaagaaatt tttaatttct
    25261 ttatcattta ttaacgattc tcttttagcc gaaacgcctt gttttgtgtt atactatgca
    25321 ttgacaagtg taaaaaagaa aaaagctcga tggactggac cgctaattag cagtccagcc
    25381 ttttgtgctg ctttttagaa ttaggtggaa aacaaaaatg aaaatgatcg tcgggttagg
    25441 aaacccaggt accaaatacc aatatacaaa acacaatatc ggctttatgg tcgtggataa
    25501 aattgcacgc gagcatcaag ctactttcaa aaaaaatccg ttcgaagcag aagttgccga
    25561 attttttcat aatggagaaa agatcctttt agtcaaacca caaacattca tgaatgaatc
    25621 tggtcgtgca gtcggcccac taatgactta ttttgggatt tatccagagg aattagtcgt
    25681 aatctacgat gatttggatc tggcagtggg aaaaatccgg ctccgtcaaa agggaagtgc
    25741 cggcgggcat aacggtatca aaagtattat ttcccatttg aatacgaatg tctttgatcg
    25801 aatcaaggtg ggaatcggca ggccagaagg gaaaaaaaca gtagtccagc atgtgcttag
    25861 tcctttcagc aaagaaaatc agcctttgat agaagaaagc atgtgtcaat ctgttaaagc
    25921 agtcgaatat ctcatagaag ggcattcgtt cgtggatgcg atgaatcgat tcaattgagg
    25981 aaggaggaaa aggaatggat attattcaat tagtcggaaa agatccgcaa gtaaaagaat
    26041 gggtcagcaa tttagaagaa aaaatgccta gacaactaat cacaggatta gcgggctcag
    26101 cgaaaacact tctatttgct cgagcgttta agaaaacaaa taaaaacatg attattctga
    26161 tgccaaattt gtattatgcg aatcagttag cagaagatct gcaacatgtg attccgcaag
    26221 atcagatcca tttatttcca gtggatgaag tgctttctgc tgaaatggcc ttttcttctc
    26281 cagaagcgcg aggcgagaga gttgcaacgc ttaacgcact tcaagaaaaa aaagcaggta
    26341 tctatttgtt gccagtcgca tctttgcaca aacgactgcc gaataagaaa acttggctac
    26401 aggcgcaatt gaattggcat attggtgatg agatagacgt ggaaaatctt cctcgccaat
    26461 tgactttgat gggctatgta cgagaagaga tggtcgcaaa accgggagaa ttcagtattc
    26521 gaggaagcat tatcgatatt tacccgttaa cgacaaccta tcctgttcgt gtagaactat
    26581 ttgatgtaga gatagattct atgcggtatt ttgaagcaga aacacaacgc tcaatcgaaa
    26641 aaacggaaga aatctgggtt tccccaacga gtgaacaagt ctacggacca gaggatctag
    26701 aacatggaat agagcaaatg caaaaaatgc tagaaaaacg gcttgctgcg accacagaag
    26761 aagcagaccg cgagtttttg caagattact tcggtcagct gacttcaagc tggcaagcag
    26821 gtgtcccgac agaagaagca aaattctact cagatcttct ttaccaagaa tctgtgacga
    26881 tcttggatta tttcccttca aatagtttac tagtcgttga tgattatcag cgaatcatgg
    26941 aaacaaatcg ggaaatcgaa agggaagcgg cagaatggca gactcaaaaa atcagcgaat
    27001 tgcgggtgtt ttctgagcag acattttcag cagatataca aaaaatcgtg cagaaggaaa
    27061 agttcaccac aacctttttc tcactcttcc aaaaagggat gggaaatctt cgtttccaag
    27121 caatccataa ttttcaatat cggacgatgc aacagttctt cggacaaatg ccattgctga
    27181 aaacagaaat ggaccgttgg cgcaagcaaa aacagactgt tgtgatcttt attccgacaa
    27241 aggacagaat acgcaaagca gaacaaatgc ttcgagatga agacatatta gtagtagaaa
    27301 cagaaaaaaa tgcattgatc agaggacagg tacaattagt tgaaggagct ttgcaggctg
    27361 gttttgaact tcctcaagaa aaaatagtag ctatcacaga aaaagaaatt ttccaaaaaa
    27421 cgacgaaaaa acaaacgaga cgtcaaacag taacaaacgc agagcgattg aaaagctata
    27481 atgagttaaa agcaggagat tacgttgtcc atgccaatca tggtatcggg aaatacattg
    27541 ggatggaaac gctagaagta gatggcgttc accaagacta tatgactatt ttgtatcaaa
    27601 acgacgacaa actgtttatt cctgttactc aactgaatct gatccaaaaa tatgtcgcct
    27661 ctgaagccaa agcgcctcgc atcaataagc taggcggaag tgaatggacg aaaaccaagc
    27721 gaaaagtctc ttcaaaaatc gaggatatcg ctgacgatct aatcaaatta tatgccgcaa
    27781 gagagtcaga aaaaggatat gcttttggac ctgatgacgc ctaccaaaaa gaattcgaaa
    27841 atgccttccc ctactctgaa actgatgacc agttgagaag tgcagcggag atcaaacggg
    27901 atatggaaaa agaaaaacca atggatcgac tactcgtagg tgatgttggt tatgggaaaa
    27961 cagaagtagc gcttcgagcg gcctttaaag cgataaagga aagcaaacaa gtagcctttt
    28021 tagttccaac aacgatcttg gcacagcagc actatgaaac gatgctggaa agatttgaag
    28081 gtttccccgt aaatatcggg ctattaagtc gtttcagaac aagaaagcaa caaaaagaaa
    28141 ccatcgaaca actgcgtaca ggtcaagttg atattgttgt aggaacccac cgaattttgt
    28201 caaaagatat tgaatttagc gatttgggtc tactgatcat tgacgaagaa caacgttttg
    28261 gtgtaaaaca taaggaacga ttgaaacagt tacgtgcaca agtggatgtt ttgactctga
    28321 ctgcaacacc gattccgaga actttgcata tgtcgatgtt aggcgtgcgg gatctttcag
    28381 taatcgaaac accgcctgcg aatcgttatc cgatccaaac gtatgttatg gaaaaaaatc
    28441 ctggggctat tcgcgaagca atccatcgag aaatgggacg aggtggacaa gtattctatt
    28501 tatataatcg ggtagaaacg atcgaacaaa aagtagaaga aatccaagaa ctcgttccgg
    28561 aagcacggat cggttatgct catgggcaaa tgacagaggc gcaattagaa aatacgcttt
    28621 ttgaatttat tgaaggtcaa tatgatgttc ttgtcactac aaccatcatt gaaacaggag
    28681 tagatatccc gaacgcaaat acgttgttcg ttgagaacgc tgattatatg ggattatcca
    28741 cgttatacca attgcgaggg cgggtaggaa gaagcagccg tgtcgcatat gcttacttta
    28801 tgtatgagca gcagaagatc cttaacgaag tcagtgaaaa acgtcttcag gcaatcaaag
    28861 attttacaga actgggatct ggattcaaga ttgcgatgcg ggatctttcc attcgcggtg
    28921 caggtaatct cttaggtgct cagcagcatg gatttattga tgcagtcggt ttcgatatgt
    28981 attctcaaat gctaagcgaa gctgtttctc gtaaacaagg aaagaatagt caagtggaga
    29041 agaccactgt tgagatcgac ctcggtgtcg atgcgtatct acctgaaaca tacgtggcag
    29101 atcaacggca gaaaatcgag atctataaac gaattcgtga acttgattcg caagaaatgc
    29161 tagatgaact agaagatgat ctgctcgacc gttttggaga atatccagaa gaagtagcac
    29221 atctcttgac aattgggcag atcaaaatgg atggcgatcg agcgctccta gaaacgataa
    29281 ggaaacagga ggcgcatata gtctttactc taagtaaagt tggaacaaaa cgctatagtg
    29341 tggaacagtt attcgaagca ctgacagcga caaaactaaa agccagtcta ggcgttgaaa
    29401 aagaacagat ggtgatacgc ttgacgatcc ccaaccaaat ggaagaagca gtatggcttc
    29461 aggaaatcca aaagtttgtc aaagcgttgc gtgagcaaaa atatctgtca gcgtaggaag
    29521 gagcaatgat ttggatcata aagaaatgcg gcgcgtcatg caaggagcat ttgtattgac
    29581 gattgcttct ttcatagcaa aagtattaag tgccctttac cgaattcccc tccaaaactt
    29641 agtcggcgac gaaggttttt atgtttatca gcaagtttat ccaatttatg ggatagcaat
    29701 gactttagct ttatccggtc ttccgcagtt catatcaaag tacgttgcag aaagaaagaa
    29761 tcctttcgag cgaaagcaag ctttgcaaaa tctttatccc ttagtatttt ggacaggtgt
    29821 ctttttgtgg ttgtttgtct ttgcatttag cagctggatt gcttggatga tgggagacat
    29881 acaattgaaa ccgctgatcc aagtagtttc ctttaccttt ttgctgatgc ctggtctatc
    29941 tttttatcga gggaatttcc aaggaagatt cttgatggaa ccaactgctg tttctcaagt
    30001 agttgagcag ttcgttcgtg tttttgtgat tgtctgtagt gcgtttgctt ttagaaagct
    30061 tggatggacg gtgtaccaaa cagggacggc agcaatgagt ggagctgttt ttggcggttt
    30121 atgcgcatat gggatccttc actactatga acaaaagatt catggaggag cgttaagctt
    30181 ccgacggttt ccattgttca aacagccgtc aaagcccttg atccggcgtt ttctggtaga
    30241 aggcggactg gtttcgattt atagtggatt gcttatcttt ttccagctga tcgactcttt
    30301 ctttgtaaaa aatgctttgg aagtttacgg actgtcagaa tacggtgcga agatagcaaa
    30361 aggggtctat gatcgaggtc agcctttagt acaattaggt ttagtgattg ccactgcatt
    30421 aagtgcgaca ttcctgcctg cgttgactcg tcatttgacc aaccggatct atcgtcagtt
    30481 tttacagaca gccaaaatct atcttagatt gacgaccgct ttagcattag ctgcgtcttt
    30541 gggattagcc ttgctgctcc cttatatcaa ttatgcgttg tttaaagatt atgcgggaaa
    30601 tgcagcgctt gtattatttg ttttttccat tgctttcact gcagtcatcc aagcttatca
    30661 aagtattgct caaagcaaga actcgtttcg cccttcttta aaaggcgcag gatggggact
    30721 attggtaaaa ggattgacta cttcttttct gaccggttta ttaggaacag caggtgcaag
    30781 cctgtctacc cttttaggat taggtgtgac gctttggtat tttgttcgaa ttgaaacaaa
    30841 agagctaaat gctttttgga aagaacggca ttttggacgg aagttgatgt tcagccttct
    30901 ttgtatgaca gtagtactgc tgctgtatta tggtgtattg aatatagtat ttggtcctgt
    30961 aactcatcgt tccactgctt tttttgtcag cgtgatagga gttgggatcg gggcttctgt
    31021 gttcatttat gcagtgattc gtcttcgtct gtttacagta cgagaatggc taatgttgcc
    31081 ttttggtaag aaaatcctgc gattaaataa aaaaatta
//
LOCUS       DAZUMB010000020        30528 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000020 DAZUMB010000000
VERSION     DAZUMB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.020"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..278)
                     /locus_tag="ACG80L_000772"
     CDS             complement(<1..278)
                     /locus_tag="ACG80L_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023050.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFV"
     gene            complement(453..2588)
                     /locus_tag="ACG80L_000773"
     CDS             complement(453..2588)
                     /locus_tag="ACG80L_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5023051.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2671..4326)
                     /locus_tag="ACG80L_000774"
     CDS             complement(2671..4326)
                     /locus_tag="ACG80L_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5023052.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4328..4642)
                     /locus_tag="ACG80L_000775"
     CDS             complement(4328..4642)
                     /locus_tag="ACG80L_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5023053.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4812..5072
                     /locus_tag="ACG80L_000776"
     CDS             4812..5072
                     /locus_tag="ACG80L_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS5023054.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5086..5400
                     /locus_tag="ACG80L_000777"
     CDS             5086..5400
                     /locus_tag="ACG80L_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS5023055.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(6130..6684)
                     /locus_tag="ACG80L_000778"
     CDS             complement(6130..6684)
                     /locus_tag="ACG80L_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS5023056.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6810..8738)
                     /locus_tag="ACG80L_000779"
     CDS             complement(6810..8738)
                     /locus_tag="ACG80L_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5023057.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(9044..9760)
                     /locus_tag="ACG80L_000780"
     CDS             complement(9044..9760)
                     /locus_tag="ACG80L_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5023058.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9761..11584)
                     /gene="glpO"
                     /locus_tag="ACG80L_000781"
     CDS             complement(9761..11584)
                     /gene="glpO"
                     /locus_tag="ACG80L_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS5023059.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11598..13094)
                     /gene="glpK"
                     /locus_tag="ACG80L_000782"
     CDS             complement(11598..13094)
                     /gene="glpK"
                     /locus_tag="ACG80L_000782"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS5023060.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13230..14690)
                     /locus_tag="ACG80L_000783"
     CDS             complement(13230..14690)
                     /locus_tag="ACG80L_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5023061.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14680..15975)
                     /locus_tag="ACG80L_000784"
     CDS             complement(14680..15975)
                     /locus_tag="ACG80L_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5023062.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16307..16984)
                     /locus_tag="ACG80L_000785"
     CDS             complement(16307..16984)
                     /locus_tag="ACG80L_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS5023063.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(17014..18105)
                     /locus_tag="ACG80L_000786"
     CDS             complement(17014..18105)
                     /locus_tag="ACG80L_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS5023064.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18206..18640)
                     /locus_tag="ACG80L_000787"
     CDS             complement(18206..18640)
                     /locus_tag="ACG80L_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5023065.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19224..20681)
                     /locus_tag="ACG80L_000788"
     CDS             complement(19224..20681)
                     /locus_tag="ACG80L_000788"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5023066.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20678..22573)
                     /locus_tag="ACG80L_000789"
     CDS             complement(20678..22573)
                     /locus_tag="ACG80L_000789"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5023067.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22631..24406)
                     /locus_tag="ACG80L_000790"
     CDS             complement(22631..24406)
                     /locus_tag="ACG80L_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5023068.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24456..25952)
                     /locus_tag="ACG80L_000791"
     CDS             complement(24456..25952)
                     /locus_tag="ACG80L_000791"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5023069.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(26146..27768)
                     /locus_tag="ACG80L_000792"
     CDS             complement(26146..27768)
                     /locus_tag="ACG80L_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5023070.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28217..29530)
                     /locus_tag="ACG80L_000793"
     CDS             complement(28217..29530)
                     /locus_tag="ACG80L_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5023071.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29775..29999)
                     /locus_tag="ACG80L_000794"
     CDS             complement(29775..29999)
                     /locus_tag="ACG80L_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023072.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(30019..30441)
                     /locus_tag="ACG80L_000795"
     CDS             complement(30019..30441)
                     /locus_tag="ACG80L_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5023073.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
BASE COUNT         8757 a         6565 c         5067 g        10139 t
ORIGIN      
        1 acaaatgagg taatcaggaa actgaaaaaa atcaatacaa aaaagccgat taagaaatac
       61 agcattttct tttctgataa aaaagcgtac aaggaaaaat ccttggctct ttctggtctc
      121 gttaatggga aaaagaccca tataaaagga agaacaacga tcatccatcc taaaagagcc
      181 actaacgttt gccaaatctt taaccagtaa ttaccttttg aaaaatactt atctttcacc
      241 aaaattgatg caacttcttc tcttgttact tttttcatat cctcacctca cgtttttttg
      301 ttacgacgta ggagtttacc atacatttca tttcttttca cataaagtgt tcctaaattt
      361 ttcctaagaa aaagaacaaa acatttctca tcagcaaatg aaaaatattt tgttcttttt
      421 atcagttgtt ctttttattt tttatctctt aattatgttg ctctttccaa tacgacaatg
      481 cttttcattc ctctagatat cgccgtgtac atttgtctgc gtcttctttg ctcatctcca
      541 tcgttttcaa atggatataa gattacatga tcaaattcca gtccttttgc tagactcaaa
      601 ggaatgattt ggcagttttc taatccatat gaattttttt taatcgattc ttgtagcgtt
      661 tgggtttcct cttctgtttg ggtgataact gccgcttttt ctccttttag agaaaccaaa
      721 atctcagcta gtattttcaa caattcttca gaggatcgaa aagagcggta ttcgggttct
      781 tttccttgcg gtcggatcga tacgatgtcg atcttctctt gatccacagc ataggttttg
      841 aaaagctctg tgattgctcc gctagaacgg taactatttc taagatcata ccgatggatc
      901 ggcagatggt agtcgtcgaa acaacgcata atatcagcaa aagtagtgga agaattaaag
      961 attgcctgat tctcgtcacc tactaaagta aagtgagttt ttggaaataa ttcgatcaat
     1021 agacctaatt gtgccagggt ataatcttgt acttcatcaa tcaatagata gcgaaatgct
     1081 ggtacattta ccttttcaac aagtaaatga cgaatagcta atataatgac tgcttcatca
     1141 acagtacgtg tgggttgcca agcataagct gagccatatc gtttttcata aattctttca
     1201 aacaactgat gctcatccac ccatgccatt tcttcgattt gtctggttat ttttcgatat
     1261 tttttttgta aaagtttcct tgcataagct acaatacttt gttccgaatc atcagaaatc
     1321 aatttaccga ataattccaa ctgcctatct tctgtcaacg agagtacctg attctgcaca
     1381 gcaggacttt ttgcttgttt caaaagatga ttcttccatt cgctcatcag tgcctttttc
     1441 gttgcttgaa gcctatcgat caatttagga tgagcaggaa ctttctcata tattttttcg
     1501 atcgtttttt tagaaaggac tgtttttcca tttttagtaa gtccacggat aaaattcgga
     1561 tgatctaaaa acaaaggatc tgattgtttt agattatcga taaacttttt gctacgcaaa
     1621 cgttctgtct gctccgatac attttctccc gtgatacgtt tgaaataagc ctcttcacct
     1681 tctatttcct ctgctgaaag ctgtgaaacc agctgtatga tggttagatt cagtggattt
     1741 ctttcaccta atgatggtag gacattcgaa atatagtcga taaaccgatg attaggtgat
     1801 agaatcagca agtcatcgga tgtcatcgta tctcgatata aatagagcag atacgcaata
     1861 cgctgcatga tagcagaggt tttaccactt cctgctacgc catttaccaa gatattttga
     1921 cttttcacat cccgaatgat tgtgttttgt tccgattgga tcgtagcagt gatagccttc
     1981 atttcattgg tctcatcttg gcctaagact tctaataata cgtcatcttg aatagctaca
     2041 gacgtatcaa aaaacttgac caaacgatct ctttccaaaa gaaattgtcg tctttcttta
     2101 ataaccgtat cgattttatt ttgacgaacc gtataagaag atggaccaat catgttattg
     2161 taaaaaagtt cggcaattgg tgaacgccaa tcataaacaa gattttcatc tgcatcatct
     2221 gtgaaatttg ctgtaccgat ataaaaggat tcttttggct cattatccag aaagtctaca
     2281 acgacttttc caaagtaggg acttttcaat aaacgttcga tctttgctag ctgattttct
     2341 gcgtttttct gtttgatatt catctgatcg atttctctat ttttcatttc aatcatcgaa
     2401 aaagtatcca ggttatctaa gtaactgtca aaattcaatc gaagctcgcc agccatttct
     2461 ttcaacgact ggtttccttc tatttttgcc gtatccatcg attcccgcag ttcgtttttt
     2521 ttatcaatta gcttttgata cacgttcttc agatatgctt cttctaactg tttttgtgtg
     2581 ttgaccatac acattctcct ctttttttaa agtggtggaa ccgattagtt tacctctttt
     2641 aagagtcgaa tgcaagcttt ttaacgagta tcatttgtta ggatatatca gttcttcagg
     2701 tcggacagtt tgatataaag aaaatgcacc atcaaggttt tttacgtggt agccagactg
     2761 cttcaagatt ctttccgcta gatagctacg taatccgcta tgacagctga cgatatattc
     2821 ttgttggctg tctaattcat ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc
     2881 ttccttgaat ttgccctttt ccaattcttc tgctgttcgc acatctaata aaatttttcc
     2941 ttttgctagt tctgtcggca attcatgcca ttgtacggtt tcactaagcc cttctgcaat
     3001 gttcattgct gcatatccta gcatgtttac tggatctttg gctgagccaa atggcggtgc
     3061 atacgtcaat tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc
     3121 taaaatatcg atccgtttat ccacaccttt tgccccaaca ccttgtgcac cataaatctc
     3181 gccagtctct ggatggaaga tcaacttcag taaaatatct gttgcttctg gataatagct
     3241 tgcatgatct tttcctgata catgaatgac ttcaaaagac agttcgtttt ctttggctgt
     3301 tctttcactt aaaccggtgg atgcggcagt cattccaaac gttcgtataa ttgctgttcc
     3361 aataccgcct tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg
     3421 acgattggct ggtgaagcaa gagaaataag tgcatctttt ccagtgattt gctgttttac
     3481 aacgatagca tcccccaccg cataaatatc aggaatattc gtttgataac gttcgtctac
     3541 taggataccc ccgcgtatgc ctaattcgat tccagcggtc actgctagct ggttatcagg
     3601 acgaacacca atggataaaa taactaaatc agcagggatg gtacgcccat catccagtcg
     3661 caatcgtttc ccgtggtctt caaatccagc gacagcatga gaagtaatca cttctactcc
     3721 ttgatgagac aattcttttt ctataaaagc tgccatttct tcatccagtg ttggaagaat
     3781 atgtggtgct tgttcgatca ccatgacttc taaacctctt ctttttaggt tttctgccat
     3841 ttccaatccg atgaatcctg caccaacgat cacggcacgc tttggctgtt tttctaatgc
     3901 atgcatcact ttatcgatat ctggtacatt tctaatagaa aaaacattgt cggcttctgt
     3961 tatccctgga atcgatggaa caactggttt tgcaccagga gataaaacca atgcatcgta
     4021 ctgttcaatg tgtttttgac cctcatgtac gacttctacc gttttgtttt cgggaaagat
     4081 ggctgtcact tcatgatttg gacgcacatc taaacaaaaa cgagcggata atgattctgg
     4141 cgtttggaca agaagatttt cccgttcact gatttctccc gaaagataat aaggcaagcc
     4201 gcaatttgca aaagaaacat acggtccttt ttcaaaaaca acgatttctg catcttccat
     4261 taatcggcga agccgtgtag cagcagacat tccacctgct acacctccga caataacgat
     4321 tttcatctta tttccctcct actgttcgat caaaccattg attcatgcca ccacgtacat
     4381 tcactacatc ataaccagat ttctttaatt ctttagctgc ttgtttactc ctcatccctg
     4441 attgacaaat cacatagacg gtcttttctt tgtctccatc atatctgttt atactttgta
     4501 atggaacatt tttagctcct tttatatgcc cgccccggta ttctgcaggt gttctaacat
     4561 caagtaacgc tggattttcc aaaagctttt cctttaattc tgggactgtt atcgatttta
     4621 ttgattgaaa tagcaaattc attttctgca cctcatttac atttttataa atacctcatg
     4681 gggtatatta aacaggagct ttattaatat gtcaaccaga atgactcttc atttttttca
     4741 ctacttttta tatttgcata aaataccgat aaggggtatt atctaattat aaaaatagag
     4801 gagaatgtgt tatggagatt gataataaaa atatcctgaa ccgtttgaaa cgtacagagg
     4861 gacaaattcg tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca
     4921 ctcaattaag cgctgttcgt tctagtattg atcgcgtaat gggcatgatc gttgcagata
     4981 atctgaaaaa ttgctttgag aaacctgata gtaatccaga agaacaagcg gctaaactag
     5041 aacaagcgat caagatgatt attaaaaagt aaaggaatga tttcaatgaa agtcgttttc
     5101 catattgatg aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa
     5161 gcttctccag aaacgacgat tgtcgtatct gtaaatggca tcgcgattac cggttatcta
     5221 gactctgcca atgcagaatt cttagatttg caaggagtcg tttttcacgc ttgcgccaat
     5281 gctatgcgtg ctaatcatat ttcagaaagt tctttacctg aacaagtaat cgttgtacca
     5341 gcaggtgtcc ttgatttagt tgaactgcaa tcccaaggtt atgcttatat caagccttag
     5401 tcagtagagg ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga
     5461 agctacttct ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat
     5521 aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttgggct tattaccgca
     5581 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aatcagcggt ttgacctaag
     5641 tattagagaa aaaaacggga aaatagctcc tcatattttg ccgttttttg agctaatacc
     5701 gaaggtcagc tgattgaaca ccgtttaagg aagagattgt gaaaaagtcg cttagtttcg
     5761 agtattaaga aaaattttgt gaaaatggat ttctacattt ttgcataaat ttggcttaat
     5821 accgagaaac tggcttttga ataccgttta aggaagaggt tgtaaaatca gcggtttgac
     5881 ctgagtatta gagaaacaaa cggaaaaata gctgctcata ttttgccgtt ttttgagcta
     5941 ataccgaagg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac
     6001 tccgaatcac aagaaataat ttcaaaaaca acttctcctg tttttggttt ggttggctta
     6061 ttatcgagaa gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac
     6121 accccttttt tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga
     6181 ttgtaaaaga ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc
     6241 tgggaaaagc agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata
     6301 ccacttcgct ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat
     6361 aaagccttct tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc
     6421 catagacaaa atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc
     6481 taagtctgtt acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc
     6541 tgagacaaca atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata
     6601 aatcggcagc aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt
     6661 acgccagtct gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt
     6721 attttagctt acacggggaa taaaaccaat gatttcactg tttttatttt tctttacagc
     6781 cactgatatt atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt
     6841 tcttcctgtc gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc
     6901 tctaggtcac gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa
     6961 cctgtcgggt acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg
     7021 attatttttt tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac
     7081 gcttcttttt ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctggcgttct
     7141 tttttattct ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc
     7201 cgataggctt tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc
     7261 attttatgag caatccacaa agcttggctt tctcctgttt ctccaacccg caactgatat
     7321 ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct
     7381 ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca
     7441 tacatagatt ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg
     7501 ccaatctcat ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg
     7561 ttttgcatat gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca
     7621 tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata
     7681 ccgaacatcg ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg
     7741 gcatttgctc cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt
     7801 accgcatgct caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta
     7861 ttgattctag gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata
     7921 tctaacaccg tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca
     7981 ccagtcaatg ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga
     8041 tagtgttcat tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt
     8101 gattcatcga taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta
     8161 tagtattcgc ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga
     8221 cgcaagaatt tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt
     8281 actttacgca aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca
     8341 atcaactctt ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt
     8401 gtctggtatt gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc
     8461 gcatattcga tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt
     8521 gcatcgatcc tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt
     8581 gcttctcttg tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata
     8641 tcgctgattc gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc
     8701 cgatcaaatt gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag
     8761 atacctgtcc caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa
     8821 aaaagtcatg tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt
     8881 tcataagcac accaaataaa ctttgtttat tggctattcg acttactgat tagataccag
     8941 ctacactcac aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag
     9001 caagacacat tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat
     9061 agacacctac cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg
     9121 cccaatctga agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa
     9181 agtcccgagc agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta
     9241 agatcaatcc acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag
     9301 ctagcaaccc aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat
     9361 aattccgtat agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg
     9421 cattccaatg tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa
     9481 cttgcgcaat gatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag
     9541 ctagagagac tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca
     9601 ttgttacagc gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac
     9661 ttttgtttaa attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc
     9721 caataaactc tcccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt
     9781 cccgcagatc tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct
     9841 cttctgtcca ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa
     9901 gctgatctcg ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca
     9961 aaaccatttc ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag
    10021 gataaggtgc catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat
    10081 cagcgatata agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt
    10141 ctgtcactgt ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt
    10201 tagagtcgat ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc
    10261 gcaaagctcc ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca
    10321 aaccatctgg ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat
    10381 cacggctgtt ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt
    10441 ccttatattc acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat
    10501 tatctccacc attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc
    10561 agcttgcttc gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat
    10621 ccaatagata atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat
    10681 ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc
    10741 catcttgctt tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta
    10801 caactaaatg gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca
    10861 agtttcgtac tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt
    10921 cgatcacttc atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat
    10981 ataaaaagcc aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt
    11041 tgatattatc aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc
    11101 cttttagccc ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg
    11161 tataattctc atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta
    11221 ctttgaccga gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca
    11281 ttggatctgg ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg
    11341 tgtcagctac tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt
    11401 tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga
    11461 ttcctgatgc tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca
    11521 acagatccaa ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa
    11581 acatatggat ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg
    11641 ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca
    11701 taaaattcgc ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat
    11761 gctgccccta gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac attcaagata
    11821 tctgtttgga attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca
    11881 ggaatcttga ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca
    11941 atcgactgta aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac
    12001 atagcccctc gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca
    12061 acataaacct catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt
    12121 tgcaacattt gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt
    12181 ccttctaaag catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta
    12241 tttttggata attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa
    12301 gtattcttga ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct
    12361 cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca
    12421 taaacttctg agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct
    12481 aaaatctcct gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc
    12541 gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc
    12601 gttccaaaca tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga
    12661 atccagcgta tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg
    12721 aaaaattctt gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat
    12781 tgccaaacaa ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt
    12841 tcacgctggt tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg
    12901 aacgctccgg caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc
    12961 caactttctt gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct
    13021 ttcttgtcaa agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac
    13081 gttgattctg tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta
    13141 tcatactact gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga
    13201 cagtttttta ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt
    13261 cttcttttca atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac
    13321 tgttttttgt ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa
    13381 cttccaatct caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca
    13441 atacgataaa tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa
    13501 aaatcgatca tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct
    13561 tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt
    13621 gttaaggaat gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact
    13681 tcgatctttc caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact
    13741 gccttttctt ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga
    13801 atgtaagtat ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa
    13861 aatatttctt cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg
    13921 tatacattca gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct
    13981 tgataaagcc ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt
    14041 ttttcattca atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct
    14101 gatgccgtaa acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct
    14161 ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa
    14221 taaaaatatc gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca
    14281 aattcctcta agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata
    14341 ttaaacgctg tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc
    14401 atcttaaact tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct
    14461 tcgtccaaca caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc
    14521 attcggccaa tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg
    14581 acatgctctc tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa
    14641 atcaggatct gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt
    14701 cgccaccttt gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg
    14761 ttcttctaac tgttcaaacg tccatccttc ttctactgca attgtcagtt ggtcaagaag
    14821 gtggaacatc tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa
    14881 tagaaatttg agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc
    14941 tgtttcaatt ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa
    15001 gaaatagcca aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga
    15061 gccactaatt gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac
    15121 atacattttt tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac
    15181 gtctttttga gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt
    15241 taattgttct tcccacatct gattgtcggg gcgagaatga ttagccagca aaacaagatc
    15301 atagttctcc tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac
    15361 aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc
    15421 tgtcatttca ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat
    15481 ttgttttttt cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat
    15541 aattgcaatt ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt
    15601 tttaactggg tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc
    15661 agtcgctaga atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt
    15721 ttctttcaat cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag
    15781 ttcttctttc gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa
    15841 attgaacccg ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact
    15901 tgttggcatc aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac
    15961 aattacaatg ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa
    16021 tcagaaaaca cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga
    16081 atcaaccctc tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc
    16141 taattgaaga gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa
    16201 aaactcgcct tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt
    16261 tttagcgtgt gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg
    16321 cttcaacaac tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc
    16381 gcccatcaat ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt
    16441 ctttcaaagc atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt
    16501 cttcattact gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg
    16561 taactggcaa caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat
    16621 agatttgtga agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt
    16681 ggccaccagc ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg
    16741 tatttcccaa ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat
    16801 ccatattcaa aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat
    16861 ctgcatcgat cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca
    16921 aacctgtagg acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt
    16981 tcattttccc atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt
    17041 atataatggg cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa
    17101 ttcatcctca cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg
    17161 tccaattgct gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat
    17221 cccaccgaca ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat
    17281 cgatttatcg ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg
    17341 cagataatct agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg
    17401 gatacctggt gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt
    17461 agcatattgt tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg
    17521 attttccaag ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg
    17581 ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc
    17641 tctacgtgtg gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc
    17701 tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc
    17761 tccagataaa gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt
    17821 tgcgccttct ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg
    17881 agcgatgctg agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt
    17941 aataacagca ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat
    18001 cactccattg aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa
    18061 ttgaactcct gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat
    18121 aattcgctct gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac
    18181 atgcaactaa tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca
    18241 ttgtctgggc taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa
    18301 gttcttccca gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa
    18361 ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa
    18421 caaaacgagc aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta
    18481 ttccatcttt ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc
    18541 ttctaaacgc ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga
    18601 aaagacattc atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt
    18661 acatatcata gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc
    18721 tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa
    18781 aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg
    18841 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa
    18901 aaactggctt ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt
    18961 aataagaaca gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg
    19021 atggtcagct gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag
    19081 tcacaaggag caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat
    19141 cgaggagttg gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg
    19201 tcacagtccc ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg
    19261 taccaatcaa atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga
    19321 tcaacataga tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca
    19381 atgcagcccc acgacgtata accaaataaa tctactccat ctttgatagc ttccttcatc
    19441 tgctcaatat gctgtttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct
    19501 tctactttgt ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta
    19561 tcatataatt gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca
    19621 cttgatggaa gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc
    19681 caattttctg gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca
    19741 acagggtagg ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt
    19801 tcttcaaaca ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag
    19861 aacaaagaag ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac
    19921 ggaacaagaa gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga
    19981 caagcttttg tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct
    20041 tgttccaaat ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt
    20101 acacagttga tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat
    20161 actgtttcag cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat
    20221 tctgtaacca aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca
    20281 tattttagac attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt
    20341 tcttcatcac catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa
    20401 cccatttcag caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt
    20461 cttttaggat aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc
    20521 atcatatgcc attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca
    20581 tcagacacag ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct
    20641 gtcgctccgc cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg
    20701 ctagttttct tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac
    20761 tatttgtaac aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat
    20821 ctacttccat caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa
    20881 agtatttgcc atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt
    20941 cttcactgac taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta
    21001 caggactgta gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac
    21061 tagcaaatgt tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac
    21121 ttaacactat atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag
    21181 ttagcataca ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt
    21241 caccaatata aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc
    21301 ctgcggcacc agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc
    21361 gtttatattt aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga
    21421 taaatgcaga taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa
    21481 gtactgctcc tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac
    21541 cgtagaaaga aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca
    21601 ctaacacttg ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa
    21661 cagctaaata tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta
    21721 ataaagtaat tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac
    21781 tttcgtgcaa gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa
    21841 ttgctggaat aaccgtactt gtataccttg ctagaatcac gggcatccct agaaatcgca
    21901 aggttatgct atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa
    21961 ccatagcgat gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag
    22021 gcaagaaata gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag
    22081 tagttaataa attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg
    22141 ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa
    22201 tgcctttttt ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt
    22261 tggataatac gtcatacact tcatttactg catttcctac tacgacttga aacattcctc
    22321 cagcttcgac tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct
    22381 tattatcttt tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt
    22441 cttcgcctcc gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt
    22501 cccccttgtt aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa
    22561 acggtttctc cattcaatta gtacccctta taatagcgaa cgaaactgta caatagtgtg
    22621 tatggtttac ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc
    22681 tcttatagtc aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg
    22741 gtatctcaag ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca
    22801 acaaagtctc ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct
    22861 gaatggaatc tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa
    22921 gcagttttgt tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa
    22981 tctcccaaga ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa
    23041 aaataagctc ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat
    23101 ttttgaaaag atacactggt tctatccggc attctttgat ccacatgttc atactttgtt
    23161 gaatctgttt ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct
    23221 ccccttgttt tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa taagcgcttt
    23281 cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt
    23341 ctaaaggata acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat
    23401 ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata
    23461 acgaaacagg aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct
    23521 ctatttcttt gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt
    23581 caattgcttg tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg
    23641 tgagggtctc taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt
    23701 ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa
    23761 caatataatt atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat
    23821 tggaaaaaat tgtactgatt tgttgaggca accttgcgtg ttggatcacc gtttctgttc
    23881 caacaagaga taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg
    23941 tcgacgaaac ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta
    24001 tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga
    24061 catacccttt ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg
    24121 aatagatttt atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg
    24181 attctaaaaa ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag
    24241 caagcatatt tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta
    24301 attcttcgct tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc
    24361 gtttatttaa gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc
    24421 tcaaatgaca atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca
    24481 aggtttttac cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat
    24541 ctcctaaagc ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc
    24601 ctcatttccc ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg
    24661 atttggcaac catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca
    24721 caacgttttg gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca
    24781 ttttcaacta tgaataatgg taattcataa cggtctgtca attcattcat gacataacga
    24841 aaccctagag gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga
    24901 ttatcgaaac catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca
    24961 gcgctacgat aataactaaa tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg
    25021 tctccatcta acattactgg tgcacagtct tcttccttcc atgtcctgta aatataagct
    25081 ggataatgcc ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt
    25141 gaaccaaata cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta
    25201 cacataatac ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg
    25261 ttcgctagaa acatatgatg acaggcttga taaatcactt tttttgttag tgtagcaatg
    25321 ggttcaagtt tatctctagc agggaatgta accaatccag cagctgcata aggcatacga
    25381 aaagcattat tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca
    25441 aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca
    25501 tactctccta ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata
    25561 tcatatcgat ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc
    25621 ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt
    25681 aaacctaact ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct
    25741 tcatgagaaa gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca
    25801 ttccactgaa tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt
    25861 aatttgccgt cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat
    25921 aaaaaatttt ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt
    25981 cttacttgaa gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga
    26041 gaagaaaaaa attgaatgct actcttcatt tttaatattt atatataaca aatacataag
    26101 acgatccccg aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa
    26161 cacaaatgct tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata
    26221 atttgacaca ataaccctta aagcttcacg tggaacagtg aacgtctgct tttcactgga
    26281 catattacaa ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg
    26341 ctcaggcgtc ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg
    26401 caaatcgatc aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc
    26461 tgcattgatt tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc
    26521 agcatgttca cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcatt
    26581 gattgattcc aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt
    26641 gattgtctca atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa
    26701 ctcttctcct tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat
    26761 cttggcactt tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag
    26821 atcgtgatta ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt
    26881 ggataatact tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc
    26941 ttcttgctga tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt
    27001 aggattggaa taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat
    27061 cagatttttg tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg
    27121 tccattactt gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg
    27181 aaagccccca atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac
    27241 ctgtttattt ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg
    27301 ccccgttttt tcatccaact gccaagcaga accactgaat attgagcgca atctattagg
    27361 ctcttttcca ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt
    27421 tttacgggcc tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat
    27481 gatgatacgg atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt
    27541 accgaattct gccatgattg cttgataatt actgatatca tacccattgt catcattcgg
    27601 agactcgtat actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa
    27661 cctggaaata atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct
    27721 tggatagatt tgataaacaa ctgaatggtg ccaccatttt tgttccatga ttttcctcct
    27781 ataaatggat gtttctattt tttgcttaat catttatttt agtctctctc actaaaaatt
    27841 gtaacgcttt caaatagcta ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca
    27901 gctgaggaaa ttccctcaac tgttttttga aaggattgat ttatgcaggg aagaatgctg
    27961 atttcacaac ttctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa
    28021 acggcaaaat atgaggaact attttttcgt ttttttctct aatactcagg ccagaacact
    28081 gatttcacaa cctctttata aacggtgttc actcagctgt ccctcggtat tagctcaaaa
    28141 aacggcaaaa tataagaaac tattttttcg tttttttctc taatactcag gccagaacgc
    28201 tgatttcaca acctctttat ttcacattca atatccacat gaatcccaca aatacaaaga
    28261 acagaccata catgattgga gatacttctt ttcctctttt agctgctaac atggtaattg
    28321 gatagaagat aaaacctaat gcaatcccat ctgaaatact ataagtcaaa ggcatgccga
    28381 tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag
    28441 attgtgccat caatactcct accataatca atgctggtgc agttacttgt gaagtaacaa
    28501 ctgatagtaa tggagaaaag aacaagccta acaagaagaa aatccctgta gtaatagctg
    28561 tgatacctga acgtcctcca acggcgatac cagctgatga ttctacaaat gcaccaacag
    28621 gagaagtacc aaataaggaa cccgcaagca tagctgttga atcagaagct aaagctttcc
    28681 ctacacgagg catcttgttg tctttcatga aacctgcttg gtttgctaat ccaactaaag
    28741 ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag cactacgacc cacatttgaa
    28801 gagaattgat gtcccctaca tgttttaatg caactaaaaa tgttggtttc aaacttggtg
    28861 ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt gaccagacct aaaatcgttg
    28921 tagctgctat cccgatgaag atgccacctg gtacacgccg aacaagcaag atagcagtta
    28981 cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct aatcctacta
    29041 atgtagattc attcgaaacg atgatcccac cttcgctcag accaaggaaa gcgataaata
    29101 gaccgatacc gccagaaatc gcatatttca aatctgctgg gatcgcatca atgatcattt
    29161 cccgcagctt gaagattgtg atcaaaataa aaatcaaaga agcaacgaat acgccggcta
    29221 atgctgtttc ccatggaatc cccattccta aacaaacaga ataagcaaaa aaagcattga
    29281 tcccaagagc aggtgccgtt ccaattggat aacgggctaa tacccccatt aaaacacagc
    29341 cgatcgcact ggctaatgca gtcgcagtaa acactgctcc ttcatccatt ccagaagcac
    29401 ctaatacagt tggatttaca aataagatat aagccattga gataaatgtt gtaaatcctg
    29461 ccagtacctc acgttttacc gtggtgttca atttttctat ttcgaagtag gaaagtattt
    29521 tttccttcat cttccatccc cctaaatccg aacattataa ataaagtccg ttaaaatttc
    29581 gatttattaa taaaacaacg tgttttatca tatactaaaa caaaacataa gtaaacaaaa
    29641 aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt atctcaaagt
    29701 tcatgaacat tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat
    29761 tctagtctca aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg
    29821 atgaattctt ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc
    29881 atttttcccc agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc
    29941 tcactatgtt ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact
    30001 gtttttcctc ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc
    30061 ttcagggtca gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc
    30121 ttccatgttc tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg
    30181 tcctaatacc tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa
    30241 ttcttttgag atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga
    30301 aatttctgca ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata
    30361 aagcatcaca cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc
    30421 ctgtctataa actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat
    30481 tcaagaaaaa taaagcaata gtttctagga ttgctttcct ccacaaac
//
LOCUS       DAZUMB010000021        29977 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000021 DAZUMB010000000
VERSION     DAZUMB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.021"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..477
                     /locus_tag="ACG80L_000796"
     CDS             139..477
                     /locus_tag="ACG80L_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023074.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="ACG80L_000797"
     CDS             complement(682..1257)
                     /locus_tag="ACG80L_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFS5023075.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="ACG80L_000798"
     CDS             complement(1989..2570)
                     /locus_tag="ACG80L_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5023076.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="ACG80L_000799"
     CDS             complement(2753..3379)
                     /locus_tag="ACG80L_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5023077.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="ACG80L_000800"
     CDS             complement(3401..4732)
                     /locus_tag="ACG80L_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS5023078.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="ACG80L_000801"
     CDS             complement(4887..5288)
                     /locus_tag="ACG80L_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5023079.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="ACG80L_000802"
     CDS             complement(5374..5580)
                     /locus_tag="ACG80L_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023080.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="ACG80L_000803"
     CDS             complement(5675..7177)
                     /locus_tag="ACG80L_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS5023081.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACG80L_000804"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACG80L_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5023082.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="ACG80L_000805"
     CDS             complement(7748..8365)
                     /locus_tag="ACG80L_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5023083.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="ACG80L_000806"
     CDS             8586..9959
                     /locus_tag="ACG80L_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5023084.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="ACG80L_000807"
     CDS             10034..11146
                     /locus_tag="ACG80L_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5023085.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="ACG80L_000808"
     CDS             complement(11218..12855)
                     /locus_tag="ACG80L_000808"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS5023086.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="ACG80L_000809"
     CDS             complement(13023..13631)
                     /locus_tag="ACG80L_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5023087.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14015..15166)
                     /gene="queG"
                     /locus_tag="ACG80L_000810"
     CDS             complement(14015..15166)
                     /gene="queG"
                     /locus_tag="ACG80L_000810"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS5023088.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15185..15937)
                     /locus_tag="ACG80L_000811"
     CDS             complement(15185..15937)
                     /locus_tag="ACG80L_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023089.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16078..17028)
                     /locus_tag="ACG80L_000812"
     CDS             complement(16078..17028)
                     /locus_tag="ACG80L_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5023090.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17029..17868)
                     /locus_tag="ACG80L_000813"
     CDS             complement(17029..17868)
                     /locus_tag="ACG80L_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5023091.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17865..18611)
                     /locus_tag="ACG80L_000814"
     CDS             complement(17865..18611)
                     /locus_tag="ACG80L_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5023092.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18849..20219
                     /locus_tag="ACG80L_000815"
     CDS             18849..20219
                     /locus_tag="ACG80L_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5023093.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20274..21170)
                     /locus_tag="ACG80L_000816"
     CDS             complement(20274..21170)
                     /locus_tag="ACG80L_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5023094.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21284..21454)
                     /locus_tag="ACG80L_000817"
     CDS             complement(21284..21454)
                     /locus_tag="ACG80L_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023095.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21451..22128)
                     /locus_tag="ACG80L_000818"
     CDS             complement(21451..22128)
                     /locus_tag="ACG80L_000818"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5023096.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22219..23124)
                     /locus_tag="ACG80L_000819"
     CDS             complement(22219..23124)
                     /locus_tag="ACG80L_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS5023097.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23299..24753
                     /locus_tag="ACG80L_000820"
     CDS             23299..24753
                     /locus_tag="ACG80L_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS5023098.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24769..25800
                     /locus_tag="ACG80L_000821"
     CDS             24769..25800
                     /locus_tag="ACG80L_000821"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5023099.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25807..26994
                     /locus_tag="ACG80L_000822"
     CDS             25807..26994
                     /locus_tag="ACG80L_000822"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS5023100.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27150..28532
                     /locus_tag="ACG80L_000823"
     CDS             27150..28532
                     /locus_tag="ACG80L_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5023101.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28504..28890
                     /locus_tag="ACG80L_000824"
     CDS             28504..28890
                     /locus_tag="ACG80L_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023102.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29090..29758)
                     /locus_tag="ACG80L_000825"
     CDS             complement(29090..29758)
                     /locus_tag="ACG80L_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS5023103.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8403 a         6140 c         5480 g         9954 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga
    13741 attatttaat atttgtaaca tttaaacaaa tatttttttg atacaaaacc ataaaagcct
    13801 tgttgtataa gcaaagtaat cgtttttcta tgttttttta tttttgaatg aaaaacgcag
    13861 attctgtaac attcactgca ataatccata aaaaaaagca aaaaaatttt tgatttttat
    13921 caaattctct taaaaaaatg taaaaaatac aaaaaaacca gtaattgtta caacaattac
    13981 tggacaaaaa ctttgaataa tgttaactat ttttttattt taactttctc cgcaataatt
    14041 tctgggcatg ttccaattcg tttataacga cttcttctgt ttctttagct aatatttcat
    14101 ctaatacatc aagccatttt tccggttctt ttgttcctag acgtccaata gcccaagctg
    14161 ctgttcctct gatgacaggg cggacatctg ttaaacattc tgaaatctca ggcaatgccg
    14221 ttcgatcacc tagattagct aaagcaatca atgcgtttct ctgcaaaggt ttcttcccac
    14281 gccatgaacc agctaaatgc ccaaattgct gtttgaagtc tttattagac atcgaaagca
    14341 ttggctttaa ttttggatat acttcgtcta cttttggttc catctcttca tgaaaatgaa
    14401 aatcttttcc ttgattgtaa gggcagacta actggcagat atcacatcca taaatgacat
    14461 ttcgcatttt tttacgatat tcttccggca tcatcccttt tgtctgagtt tgataagaca
    14521 aacatcgctg ggcattcatt cttccgtcac caagcaatgc ttttgtcgga caagcagtca
    14581 cgcagcgcat acagtcaccg caaccaaatt ctcctggttt atcaggctca aatacaatat
    14641 tagtaacgat ctcaccaaga tagacaaatg aaccgaactc ttcagtgatc aacaatccgt
    14701 ttcgtccaat aaatcctaaa ccagctcgct gtgctacagc gacatcgatc agttctccag
    14761 tatctacttg cggagctaga cgccattcct cttctttttg ccatttttct gctcttgttt
    14821 ggatgaattc gatcaatcgg ttcaaacggt ctctgagtat atcatggtaa tcgatccccc
    14881 aagaagcacg agcaaactgc ccgcgttttt catccctagg cacttcttca tggatcctag
    14941 taggatacgc aagtgcaatg gaaatgatcg atttcggctg atcaaatgtc atttctggat
    15001 aaagacgctc atcgatattt ggatgttcaa agccagaagt atggccagct gctttttgtt
    15061 cttccaacga ttcttttaat gaatcaaaag gttccgccgt ggtgaacccg attttgtcaa
    15121 ttcctaatcg tttgctttct tgtatgattt cttcttttaa tgtcatggtc ttcctcgctt
    15181 gctgttaacg gctgtataac ccataatata gtttttcttt tttcagctgg acaaactgga
    15241 tctgctgatc ggcaatcttc ttttcttcca tccatttttt tcttgcagca aatacttccc
    15301 aataatttgt tgaaattaat tttcttttgt tatctaatgc attaaaaaaa ttcaatgaaa
    15361 ctggttttgt acttaacagt tcatggagcc ttacctcttc ccatggcgaa aactcagaca
    15421 gatcgcggga atacaatact tcttcataga gatttttttg ctgtgtgtac gtgaatgttt
    15481 gcgggatttt cccaggccta gcaatttcta ctagacgatt ataaaacata ttcattaatt
    15541 tgctgttggc aataaattga cttttataat aggccgactg gatctttttg attactcctt
    15601 ctgctttgat gactgaaaat aatgcaggcc atgctttgac ggttatttcg tcgttttgtt
    15661 taactaatac gccaggtgtg aagccgactg gagaaaggaa cacaaaataa ccgataccgg
    15721 tattttcatc taaaggctga aattcatcaa acaaccattt gcctatgtac agctcttcat
    15781 ctggttctgt tgtctcattt tgatttgcta tgaataattt agctaattct gcttcaagaa
    15841 attgaaggtc ttgatccttg tcggtatttt cctgctggct gatcaatcga aagacttctt
    15901 ctttaatcgt atatttatct ttacgtgtaa aattcatagc cacaccttcc ttccttactc
    15961 taactataac ggaatttctt caaaaaaggt atctgtacga cgaaaaacag cataattttt
    16021 tttcatatct cttataaaaa atgcaaggtc tgctcctcaa gctagacctt gcacttttca
    16081 gctttctttt gtttgtgaca aaccttcatg gatcgtttca atattccatt tcatcatctg
    16141 atagtaagaa tctccttgtt ctccttcttt tgctatggaa tcagtgaaca gtttatcata
    16201 gattttcaaa cccgtttctt ttgctacccg ttccatgctt cttgagtcta cactggtttc
    16261 cacgaataaa accggtactt cttttgctct tatctgatca atgatcgctt tcatttgatc
    16321 aggcgtacct tgactttctg tattgatctc ccatatataa gctgctggca aatcatatgc
    16381 tcgtgaaaaa tacttgaatg ccccttcact tgttactaat aattttttgt tctcttcgat
    16441 agaagcaaaa ctttccttcg cttgtgtatc taattctttt agtttttcca catatgcttc
    16501 tgcatttttt ttatagagtg tcgcattttc tgcatctttt tcagagaata tacgtgcgat
    16561 ttcctccaca tattttattc cgttagatag gtctagccaa gcatggggat ctgcttttgt
    16621 atgttcttca ccgctagtta aataaagagg ttctacattt ttgctaactg caaaataatc
    16681 ttttccttct tcttttttag ccgtttccat caagttatcg aaccagctgt tacctgtttc
    16741 aagattcaaa ccgttgtata aaataacatc tgcatcgctt gcctttttga tgtcttctgg
    16801 tagtacttca tattcatgcg gatctgttcc gactggtacg atactgtgga tatctatttg
    16861 atctgttccg acctcttttg ccatgtccgc caagatcgaa ttggtagcca cgactgacaa
    16921 cttttcttta cttccttcat ccgcttggtc agaagcctgc tttccgcaac cggctaatac
    16981 caagaaacta actgccaaga ttaaaaagag tgattttttt gttttcatct atctgcttcc
    17041 tttctgaaat aaaattcctt ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt
    17101 acatgctaac acgatcgtag ctcctgatgg ccaattgtaa ctataactga aaaacacacc
    17161 gacaactgca cttagcatac cgattcctgc agctgtaatg atcattttca gtaaatggtt
    17221 cgttagcaag taagccgtgg cggctggtgt gatcaacatg gcaatcacca agattgttcc
    17281 tactgtctgt aaactgacta cagccactaa tgtcaaaaag aacatcaaaa gataatggat
    17341 cagcgatgtg ttcagcccat aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa
    17401 ttctttataa aagaaaaaga caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa
    17461 caaatcgctt tccctaacag ctaagacatt cccaaataaa atatgataca aatctgtcgc
    17521 actctgggca tatgaaatca agataatccc taatgcaaaa aaggagctaa agacaattcc
    17581 aatcgctgta tcatttttca atcggctccg ctgagtcacg aaaccaatca ctgctgctgt
    17641 tagcatccca aaagcagtgg ctccaaagat gtaactaaaa ccaaacatat aagaaatggc
    17701 tacacctgga agaactgcgt gagagatagc gtctcccatc aaagacatcc ctcgtaaaat
    17761 aataaaagaa ccgatgactc cagaagcaag tcctacaagc atggaagtga ccaaagcgta
    17821 ttgcaggaat tgataattca ttaatccatc aataaaggat tggatcattt tttctctcct
    17881 ccttttacta aatgtctgat gatctcacca taggccaatt gaaggttctt cgatgtaaag
    17941 acttcttgta ctggaccgga agcgatcagc tgtttattta aaagaataac ctcatcgaaa
    18001 tattcttcta ctttatgaag atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc
    18061 aattcctgca agatgtcaaa gatctttcgt tcacttaacg catcaatccc tacgaatggt
    18121 tcatctaaaa agatccattc tgctccttgg gctagagctc ttgcaataaa aactctctga
    18181 agctgtccac ccgatagttc gctgatctgt cttttggcat attcttccat ccctactttt
    18241 ttcaaagctt gtcttgcacg ttctttttct tttttcccag gtctttgtcc aattcgtaaa
    18301 gatggatatg ttcctaaaag tacaacgcct aatacatcaa ttggaaagga aagatccaat
    18361 tcacttcgct gttccacgta ggctattttt ttacggactg agtttaccgg ctgtccagaa
    18421 aaggtgactt taccagtagt agctggtatc aactctagca aagatttcat aaatgttgat
    18481 tttcctgcac cgtttggtcc gataataccg gtaatttttt gtggatttat cgtcaagttg
    18541 atagttttca aaacagtttt cccttgataa gcggctgaaa ggtcttgaat tacaatggcc
    18601 gtacgttcca aatcttacac tcctttttag gtttacctaa ctttttattt aaatatagtt
    18661 taactaaaat ggaaagcatt ttcaagtaga atgatgaaaa agtttttttc gaattttcct
    18721 cgcattttat gaaaattttc tgtagaatca catcaagagt tcaataaggc taacctaata
    18781 atgaaaaaca tgtgttgaaa cgtttgatta ttttttcttg ttgactcaac aacgaaagga
    18841 gtttttgtat ggaagaagaa aagttaactc gaagtcttag tgcccgtcat atccaaatga
    18901 tcgcactagg tggaacgatc ggtgtcggtc tgtttatggg tgcctcctca accatacgtt
    18961 ggacagggcc ctcagtgatg ggagcctatg cgattgcagg attctttttg tatttgatta
    19021 tgcgtgcttt aggcgaaatg ctttacgttg atccttcgac tggttctttt gcaaattacg
    19081 cgagcgagta catccatcct gtggctggct acttgaccgc ttggagtaat atttttcaat
    19141 atattgtcgt aggaataagt gaagtgattg ctgtaggatc ttatatgaat tattggtggc
    19201 ctgatttgcc ggcaattatt cccggaatca ttgtcgtctt gtttcttatg ctggcaaatc
    19261 ttatttctgt caaggcgttt ggcgaattag aattttggtt ttcgatcatc aaagtcataa
    19321 cgatcatttt gatgatcatt gcaggtcttg gggtaatctt atttggattt ggtaatcatg
    19381 gtcaggcagt agggatttca aatctttgga aaaatggtgg attcttcact ggaggcgtca
    19441 aaggattttt ctttgcatta tcgatcgttg ttgcttccta tcaaggaatc gaactgatcg
    19501 gaatgactgc tggtgaagca gagaatccta aaaagacgat cattgaagct gtccagtcca
    19561 ctatcggtcg aatcttgatt ttttatattg gtgctatttt tatcattgtg agtatctatc
    19621 cgtggaatca attgagtgag attggttcac cttttgtaca aactttttct aaagtcggca
    19681 ttacatttgc tgcaggactg attaattttg tcgtgattac agcggcatta tcaggttgca
    19741 attcagggat tttcagcgca agtcgtatgg tctatacact tgccataaat ggaaagatgt
    19801 ctaaaaaatt tctgaaacta tctcgtcacg gtgcgccttt ttatcctgtc gtagcaatct
    19861 caggtggaat cttaattggt ctgatcttaa attatcttct accaatgttc atcaaccagt
    19921 cagaaaatat tttcgttttc gtatacagct caagtatttt gcctggtatg gttccttggg
    19981 tagtgatttt gatcagcgag attcgcttca aaaagaagca cgcagataaa atgcatgatc
    20041 atccttttaa aatgccacta tctccttata cgaattatgt gacacttgct tttttagcta
    20101 tcgtattgat tttcatgttt atcaatcctg aaacgagaat ctcattgatt attggactcg
    20161 ttttcttgct ttacatgacg atccattact ttgtacgcga aaaaagaacc ggaaaataaa
    20221 aaaagaacga agcttcacct tagtggaagc ttcgttcttt tttattgctt ttatcaattc
    20281 aattttagcg tctcaccggg agtcaggaaa taattctcca tgctcatccc attcagcgac
    20341 aagatcgttt ctactggtac gccagttcgt tcggctattt gatttggacc ttctccttcc
    20401 aaaactacgg ttgtcgcagc ctgtgaagtc gcgctgttgg tattagtttc agaggaatct
    20461 gttgtgtctt gtgttgttcg agcttgcatt tcagaagctt ctaccgactc tgctgtctca
    20521 gaatcagaag atgttgtgtc ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc
    20581 tgcaaataaa tatcggaaat cttagcgaca tgattcgtat aattagccga taattgattg
    20641 atcaaagtat ctaagtctac accgtcttta ttttctgctg cctgatacgc tgcacgtaat
    20701 tttctgatcc cttcgtcgga gattgcttga agctcacgtg ctttttggtt agcaactgct
    20761 gataaaccag ctattccctc ttgatttccc tgaatttctg tctggtattc ttcaatcaat
    20821 gcaggcgtct tttcttcgat ctttttcgaa taatcattta cgattgcttg aagttgctct
    20881 ggcaaaccat ttgctgtttt ttcttcagaa gtttgtgtct cttttgtttt agttgatgac
    20941 tcacttgttt gtttcgtctc tgttttcttt gtcgttgttt ccgttatggt cgtacttttc
    21001 gcttctgacg aattttttcc atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa
    21061 aacacggcaa ctgctgcccc aatcatgatc atccgcttac gagctgcctg tttttgctgt
    21121 cttcttcggg tcatccgaac attttctccc cactcatttt catcatacat ttttttcacc
    21181 accaattttt atgtataata gcgttatcaa acgcttcata ttcttattgt agcaaataat
    21241 tgtcgctgtg cacattttta tctttgttat ttataagaaa atttcaccag ttgagatgat
    21301 tccaagattg tccgtcgatt tctccttgca gctgcattgt tcgttcgtct atctcagcca
    21361 tcagtttctt tatttcatat aagagcgcac ggtcttgata ttctgatttt tcgcttaatt
    21421 catctaattg ttggatcagc caatcatttg tcataatgaa gttcctttca tttcttgcag
    21481 tgtaaaggtc aagtcttcga tctgctcttt tgcctcagtc agtgcttcta cgatatatgc
    21541 ttgtttttca gattcttctt cctgcatttt ctgattttcc tgtactcttt gaataaacca
    21601 gtcaatccgc tgagtcagcg atggtttgcc ttccaatgag agatactgct gataagcaat
    21661 tagcttctcc caacgttttt cctgggataa atacataaat tgtgctaaaa cgaatggcgg
    21721 aagataggtc atatggaatt tatttgcgat tcgctgatac ggcctcaaaa attcggaaat
    21781 cgtatattct tcttttcctc cagcttggta ttctttttcc gccacacctg ttgtcaatac
    21841 gattcctagt tctttccctt ttaatacatg tgctgctcgt tccaagacat tatcttgcca
    21901 aatttttaaa tgagcaggag aactatacca gtaaaacgga aactggaaaa tgatccgatc
    21961 atgctgggcc aagagctgta gttcagtctc tatatctatt tttccgtctg gataacttga
    22021 ttccaaatga tgccaagtag ctggtaaacc agcagaagct tctttgaaaa attgctgatt
    22081 taaagattct tcgatcgttg gatggctaat gatgataagt gtacgcatcc cttttcctcc
    22141 tttttagtct tcgctttaca gtgtagcata accgccacaa gtttcttttg ccgatgcttg
    22201 tcattttaac cagactaatc aggttgtcca agaaactgcg gtttgttttt ccaactgttt
    22261 tttcaaggta tccaaagcaa tattcttaaa attttcgttt ttcatacgtt tacgatagtt
    22321 cgtagccgtt tctccggtac tctttttgaa cattcggcta aatacgacta gactattgaa
    22381 ccctacttga taggcaatac tttccacgga ttcatccgtc agagaaagaa gaaacgcccc
    22441 tcgattgatc cgtaccccga tcaaatattc cttgatactg gtacccatat gatttttgaa
    22501 tactcgcgac aaatggctcc gattgaccgc taaatgctgg gcgatttcct caacagacaa
    22561 tttttcagca taatgtgcct cgatgtattt gactgtttca acagctaaag aggaatgaac
    22621 cttttgtaca ccaccgctga cctttccgcc atcttttaaa aggactgata aaaaacggta
    22681 ggttaatgct gttaactgta attcattttc caaggtttct tccgtatctt tcatgcattc
    22741 taaaataatg tcgatatatt tttgtatgtc agaagagacc atcgtgtatt ggtcttctct
    22801 gaaaagcgac tgccgaatga tttcttctgc agcactccca ccaaatgaaa gccagcaata
    22861 catccacgga tcttctccat ctgataagta aaacgtcgag tctcctggac gaatcaagaa
    22921 cagatcacct tttttcaatt ggaattgttg atttttcaca tgataagatc ctttgccttc
    22981 catcacgaca tgcaggatgt aatcattgcg aatagcagga ccaaaagaat gaaacggcat
    23041 cgttttagag aaaccacaaa aatcaaaata caattctttt tctacttgtt gatgttcctt
    23101 ttttaaaaaa atcgattgct ccacgtctat ccctccttac tcctttagta taatgcaaca
    23161 aaaagttaaa taaaagtcac agaaatatat taaaaaggaa gcgatttctt gttaagttta
    23221 agtcattaaa agatctttgt tattcgttgt aaccggttta cttagtatta tatattgaac
    23281 aggagaggaa tacagattat gaaaagcaaa atgatccaac gtgtgtccta cgctttaggc
    23341 gcttttggac acgacacatt ctatgttaca ttaagcactt attttatggt attcgtgaca
    23401 agccagatgt tcgcaggaac agataaggca acgaatgcaa aaatgattgc tacagttaca
    23461 agcttagttg taggtatccg cttagttgaa attatttttg atcctttgat tggtggcatc
    23521 atcgataata ctcgtacaaa atacggtaaa ttcaaaccat ggttagttat cggcggcgtt
    23581 gttagttccg tactcttggt agcaatcttt accaatttct ttggcttagc tactcataat
    23641 acgactttat ttatcatatt atttgcaatc gttttcatct tattggatag tttttattca
    23701 tttaaggata tcgctttttg gtcaatgatt ccagctttga gtacggaaac aaaggaacgt
    23761 gaaaaatttg ctacgtttgc tcgtttcggc tcttctctag gtgcaaatgg tactactcta
    23821 gtcgttgtcc ctatcgttac tttctttacc tatcttgtta ctcataaaca tattgaaggc
    23881 gcttcaggct ggttctggtt cggtgtgatt gctgctgtta tttcaggcgg tactgcgatt
    23941 atcactgcct tgggcacaaa agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct
    24001 gggatcaaag atgtgttcaa agcagttgct caaaatgatc agttgatgtg gcttgccctt
    24061 gcttatatcg cttatgcgat tgccaatgta gcaacaactg ccgtattatt ctacttcttc
    24121 aaatatgtac ttggccaacc aactgaattt tggatcgttg gtctagttgc tgccttactt
    24181 ggtttagtag ctgttccact attcccaatg cttacacgcg tcatctctcg tcgctacgtt
    24241 taccttggag ggatcgcaat gatgatttct tcttactttt tctttacgat cgcaggttca
    24301 agtttgccaa tcgttatcat cgggctagta ttgttctact tccctcaaca attgattttc
    24361 ttatccgctt tgatgacgat cacagactct gttgaatatg gtcaatggaa aaatggtatc
    24421 cgtaacgaag ctgttactct atcaattcgc cctttactag ataaaatcgc tggtgctttg
    24481 tctaacggga tcgtagggtt tgtcgctatc gcttctggga tgactggtaa tgctactgct
    24541 gctgatatca ctgctcatgg cgtaacgact ttcaaagctt atgcattcta tgctcctgct
    24601 gctttgatga ttttctccgc tattattttt gcttggaaga ttacattgac agagaaaaaa
    24661 catgcacaaa tcgttgagga attggaaaac aaattggctc cagctgatac aatggaagat
    24721 gaacttcacg atgcagtagt tccttcccat taagaaagga tcttgaaaat gaagattaca
    24781 gaaaaagatt ttggccaagg ttatcacttg ataaccttgg agaacaaaaa caagctagca
    24841 ctttctatct ctgatttagg tgctagaatc gtcagtttaa aatcaaatga tcgagaatta
    24901 gttttgggat ttgacaccgc tgaagaatat atcgaaaaag atccgtatat cggtgcttct
    24961 atcggtcgca ccgcagggag aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac
    25021 caattagcca cagatccaaa aactggacat aatctgcatg gtggtgctcc tggatttgaa
    25081 ttaaaaaaat ggtcttatgt cattttgaat ggcgaaaatg aagcttctgt catttttact
    25141 actacaagtc ccgacggaga acatggtttt ccaggtacaa tggatgtgga aatccgctat
    25201 actttgacaa aagataatat ctggcgagtg acctctcgcg gcaccagtga ccaagatact
    25261 ttattcaatc cgacaaatca tgtttacttc aacttgacgg gagatgcttc tcagtcaatc
    25321 gatcaacatg agctttggct caatagcgaa gcttatgctc ctttgcgaac agatagtatt
    25381 cctatagggg taaaagaaaa tgctgcagga agtgctttcg attttcaaat acctaaaaaa
    25441 ttagcgtccg tttttgctag tgatttggat caaaaaaatc ttgttgacgg aatcgatcat
    25501 ccatttttct taaaagaaac cggcttgggt aaagaagccg ctcgcttgac tagccccgat
    25561 aaaagaatcc aagtaggtat tgctacagat gcttctagtg tggttatttt cactgcaaat
    25621 ttcggcacag aaacaccaga aatgcgcaac cgaaaactgg cacatcacgg tggcatcaca
    25681 tttgagactc aaacagctcc aggtgcagag cgcttctcag catttggttc gattcattta
    25741 aaagctggat cagtatttga aacagtgaca gaattcaaaa tcaaaacaag aaaggaataa
    25801 ctcatcatgg aaatcaaaag aagcttaaac acaaaatttg ccgaaacatt cggacctaaa
    25861 aaaactacac aatacttctc accaggacgc atcaatttga ttggtgaaca cacagattac
    25921 aatggaggcc atgtcttccc cgcttccatc acttatggaa cgcaaggtgt agcagcacct
    25981 cgagacgata ataaaatatt agtttattcc acaaactttg acgatatcgg tgtcattagt
    26041 tttactttag atgagctaga atatgataaa aaagcaaact gggcaaatta cgtaaaagga
    26101 atggtcctta aattaaaaga agctggacac tcaatcgatc atgggtttga gttgttagtg
    26161 gaaggaacaa tccctaatgg tgcaggactt tccagtagcg cttcattaga attattagta
    26221 ggtgttgtat tagaagatct gtttgattta aaaacagatc gcttggaact agtccaaaca
    26281 ggtaaaaaag tagaaaatga attcatcggt gtcaattcag gtatcatgga tcagtttgcc
    26341 atcggcttcg gtgagctaga tcatgcaatc ttattagata caaatacgct gaaatacgaa
    26401 atggttccag tgaaactaga tggttatgca atcgtcatca tgaatacgaa taagcggcgg
    26461 gaattggcag attcaaaata caatgaacgc cgtagcgaat gcgaagaagc cttggcacgt
    26521 ttgcaaaaaa ctttgcctat ctcttcttta ggagacttag atgaagaaga atttgaatca
    26581 actattgacc aaatcggcga tgatacattg atacgcagag ctcgccatgc cgtttctgaa
    26641 aatcagcgga ctttgaaggc aaaagctgaa ttagaagctg ggaacttaga agcatttggt
    26701 caactgttaa atgattctca ccactctctt cgctatgatt atgaagtaac tgggatcgaa
    26761 ttagatacac tagtagatgc tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca
    26821 ggagctggat ttggtggctg tgcgattgct cttgtcaaag aagaaaatat acctgccttc
    26881 aaaaacaatg tctatgatga atacatgaaa gttgtcggct atgctccgga attttatgtt
    26941 gcacacattg gaaatgggac aacaaagatc gatccagaag ctgttcatgt ttgataaaaa
    27001 aatcaacgat ctcagctaaa aacaaataga taaaaataac ctctcagcaa tttccaaatg
    27061 tgagcctttt gctcgtattt gaaaattgct gttttttatt gacacggaaa gagaacgtgt
    27121 gttctaattg tgttgaagag gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt
    27181 atgaaaaaga accttcaaga gatattttat gtattgactg caaatctttt tacgcctccg
    27241 tagagtgcgt tgaaaaggat cttgatcccc ttacaactaa attagtagtc atgtcctatc
    27301 cgtccgattc gacagaaaca cggggtagcg gattaatctt ggcttccagt cctgcagcaa
    27361 aaaaagctta cggcatcacc aatatcagcc gtgccagaga tcttcccttc ccctatccaa
    27421 gtgatttata tattgttgcc ccaaggatgg cctattatat ggaaaagaac agagaaatca
    27481 attctatcta taaaaaatat gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa
    27541 gcttccttga tgtaacaaat agcatggcac tctttggtaa aacaaatgcg tacgacatgg
    27601 ctaaactgat ccataaagat gtgtttgagc agaccggaat ctacacgact ataggaattg
    27661 gcgataaccc gctacttgct aaactagcct tagacaatgg ctcaaaagac ggaaagcaaa
    27721 tgtttgctga atggcgttat gaagatgtac cagaaaagtt atggaaaatc tcagatatca
    27781 ctgacttttg gggaatcggc aaacgaacag caaaacgtct gaaacaaatg ggtatacatt
    27841 ctgtttatga tttagctcac gcggattatt atcacttgaa aagcaagcta ggggtcatcg
    27901 gtactcagct ttatgcccat gcttggggca tcgatcgaag ttttttggga caagaatata
    27961 caccaaaatc aaaaagtatc ggaaatagtc aaatcctaga tagagattat acaagacgag
    28021 aagaaatcga gatcgttatc aaagaaatgg ccgaccaagt cggcactcgc ttgagacgat
    28081 ctgcagcaaa aacacaagta gtcagcttat gggtaggata ctctttaggc tatcttgatc
    28141 caaatggcaa gaccgggttt caccagcaaa tgaaaatcga agcgacaaat gctagcagag
    28201 atctagcaga tgctttattg accattttcc accagcatta ccactaccag gatattcggc
    28261 gtgtaggcgt gaactgttcc gacctcgtat acgcgacagg tttacagctt aacctttttg
    28321 aagatcctga aaagcagata aatgaagcga aagttgattt tgttgtcgat acgattagaa
    28381 aaaaatacgg ctttcaatca atcatccatg cgcatagtct cttgcaagga ggacgcgcca
    28441 tcgcacgtag ttctctcgtt ggcgggcatg caggtggtat gagtggatta gaaggtggaa
    28501 aagatgagac ggacagcaaa aaaattcatt gactataacg agtatcgaga tcggccattt
    28561 ggattaaaat gggggactgc ttttgcgatg gaagaactag tcaacggtat caaaaaaaat
    28621 gaagaagaag cacgcaaaca gcctcttcct aaacaacaaa tgacattgca agaaatcgat
    28681 agtatactga cagaagcctt tttatctcgt aaagcagtaa tcattcagct caatttaaaa
    28741 gatgaatttg gaagactctt ggatgaggtc gaagggcact tcattggtga agcctatgaa
    28801 gattacttta tgattgacca tcagccgata ttgtgggaag acgtacggta tattgagtta
    28861 aaggaaacaa aaaaatggtt tgtgacatga gaggttgtga cagaagtgat caacttcaaa
    28921 aaataagaaa ggatttttcg aaaattgctt cttaaatttt tgtggaattt tggcttattt
    28981 ttcgaagaag caacttctgg aacaacgtat attagatatc agaggttgtg aaaaagtcgc
    29041 ttagttttga gtaacaagcc aaggcgctga tttcacaacc tctgtctatc tatccatcta
    29101 ttcggtaaga ttcatgcatg acgtcttctt tatttagtat gtagagccct ttttcttctg
    29161 accattcaac ataaatcagc tgttttacat ctgcatagcc ttctttcttc aagttctcca
    29221 taagccatgc ttcagtcaat tgattggact gaagcacttt atgctgaatt tgcccttcat
    29281 ctacaagcag gatcgataaa gctttgtctt catctgattt tttcaataca ctcatttgac
    29341 cagcattctc taggataaca tgtttggcat tttcaagaga aaagcattct tgctgtctta
    29401 agattgctcg caactgctcc atctcgatat ggttatttgt caactctgtc atgttgatga
    29461 cgccgtcttt gattagaaca gatggctcgc ctttgaccca acgattgacc ttttcatttt
    29521 tttggacgat acgttcgata ccataaatca ttactgccca aagcgcaatg gcaaatagga
    29581 catgccctac atggacattg tcatcatagg ttgattcttc taagatccca cctaaaacaa
    29641 gcgtatagac taaatcaaac ggtgtgattt ctgacatgga tttcttccca agcaaacgta
    29701 ccactaataa taaaccgatc aacccgaaca ccagtttcat tgtaattgaa aaaatcattt
    29761 tttctccccc taactatctg ttaattaaat tatatatgct ttctcacgaa cacgcatgga
    29821 atatacaagg aagaaatgta tccttttact aaaaaaagga ctgtgacact gtagtgaccc
    29881 ccaaaagtta gacttttttg gagtggagaa atccactcct tttttttatg ctctaaaatg
    29941 atatttttac acaaccgtta cggttgtttt acgaaat
//
LOCUS       DAZUMB010000022        28421 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000022 DAZUMB010000000
VERSION     DAZUMB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.022"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1654
                     /locus_tag="ACG80L_000826"
     CDS             17..1654
                     /locus_tag="ACG80L_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023104.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1799..2539)
                     /gene="truA"
                     /locus_tag="ACG80L_000827"
     CDS             complement(1799..2539)
                     /gene="truA"
                     /locus_tag="ACG80L_000827"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5023105.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2730..3365)
                     /locus_tag="ACG80L_000828"
     CDS             complement(2730..3365)
                     /locus_tag="ACG80L_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5023106.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3512..4732)
                     /gene="sstT"
                     /locus_tag="ACG80L_000829"
     CDS             complement(3512..4732)
                     /gene="sstT"
                     /locus_tag="ACG80L_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS5023107.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4951..5949
                     /locus_tag="ACG80L_000830"
     CDS             4951..5949
                     /locus_tag="ACG80L_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023108.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6145..7980
                     /locus_tag="ACG80L_000831"
     CDS             6145..7980
                     /locus_tag="ACG80L_000831"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5023109.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8040..8756)
                     /locus_tag="ACG80L_000832"
     CDS             complement(8040..8756)
                     /locus_tag="ACG80L_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5023110.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8983..9525)
                     /locus_tag="ACG80L_000833"
     CDS             complement(8983..9525)
                     /locus_tag="ACG80L_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS5023111.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9530..9757)
                     /locus_tag="ACG80L_000834"
     CDS             complement(9530..9757)
                     /locus_tag="ACG80L_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS5023112.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9836..11716)
                     /locus_tag="ACG80L_000835"
     CDS             complement(9836..11716)
                     /locus_tag="ACG80L_000835"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5023113.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11905..12519)
                     /locus_tag="ACG80L_000836"
     CDS             complement(11905..12519)
                     /locus_tag="ACG80L_000836"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5023114.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12661..13221
                     /locus_tag="ACG80L_000837"
     CDS             12661..13221
                     /locus_tag="ACG80L_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5023115.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13526..13840)
                     /locus_tag="ACG80L_000838"
                     /pseudo
     CDS             complement(13526..13840)
                     /locus_tag="ACG80L_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14094..15729)
                     /locus_tag="ACG80L_000839"
                     /pseudo
     CDS             complement(14094..15729)
                     /locus_tag="ACG80L_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16284..16847)
                     /locus_tag="ACG80L_000840"
     CDS             complement(16284..16847)
                     /locus_tag="ACG80L_000840"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5023116.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16862..17905)
                     /locus_tag="ACG80L_000841"
     CDS             complement(16862..17905)
                     /locus_tag="ACG80L_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023117.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(18038..18442)
                     /locus_tag="ACG80L_000842"
     CDS             complement(18038..18442)
                     /locus_tag="ACG80L_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5023118.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18675..19310)
                     /locus_tag="ACG80L_000843"
     CDS             complement(18675..19310)
                     /locus_tag="ACG80L_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5023119.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(19576..20628)
                     /locus_tag="ACG80L_000844"
     CDS             complement(19576..20628)
                     /locus_tag="ACG80L_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5023120.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(20641..21579)
                     /locus_tag="ACG80L_000845"
     CDS             complement(20641..21579)
                     /locus_tag="ACG80L_000845"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5023121.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(22030..22680)
                     /locus_tag="ACG80L_000846"
     CDS             complement(22030..22680)
                     /locus_tag="ACG80L_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023122.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(22780..23289)
                     /locus_tag="ACG80L_000847"
     CDS             complement(22780..23289)
                     /locus_tag="ACG80L_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS5023123.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(23490..24359)
                     /gene="scrK"
                     /locus_tag="ACG80L_000848"
     CDS             complement(23490..24359)
                     /gene="scrK"
                     /locus_tag="ACG80L_000848"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5023124.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(24379..25500)
                     /locus_tag="ACG80L_000849"
     CDS             complement(24379..25500)
                     /locus_tag="ACG80L_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5023125.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(25502..25897)
                     /locus_tag="ACG80L_000850"
     CDS             complement(25502..25897)
                     /locus_tag="ACG80L_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5023126.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(25916..26713)
                     /locus_tag="ACG80L_000851"
     CDS             complement(25916..26713)
                     /locus_tag="ACG80L_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5023127.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(26715..27503)
                     /locus_tag="ACG80L_000852"
     CDS             complement(26715..27503)
                     /locus_tag="ACG80L_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5023128.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(27517..27993)
                     /locus_tag="ACG80L_000853"
     CDS             complement(27517..27993)
                     /locus_tag="ACG80L_000853"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5023129.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(28065..>28421)
                     /locus_tag="ACG80L_000854"
     CDS             complement(28065..>28421)
                     /locus_tag="ACG80L_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5023130.1"
                     /translation="NNILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFD
                     NNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVD
                     IPIHEVKYVYDYVKRA"
BASE COUNT         8948 a         5705 c         4377 g         9391 t
ORIGIN      
        1 taaataggag aaaaaaatga aaaaaaacaa aaaccaaata atagatatca tttttatgtt
       61 atttctaagt tttgcttacg tcatccctct attcatgtct aagggaatct atcatagtgt
      121 caatcaggat acttactttc atttatctcg aatcatagga ctagataatg tatggagttc
      181 tccagtaaac ttcaataact tcgatcatca tggaacaatg atgaatattt tttatccatg
      241 gcttactctt tatccagctt ttttatttta taaaatgatg ggaaatcttg ttttaagtta
      301 taatatctac tattttttta tcactttttt aacaatggtc gtttcttatt tttctatgaa
      361 acaaataaaa aacaatcgtt atatttcgct gttattttcg attatttata cattttccgc
      421 ctatcgtgcc attgacatat tccatcgtgc ttcattggga gaagctgtcg ctttaacatt
      481 tttgcctttg atactaatgg ggtgttacga aatttatatt agagattacc aaaaatggta
      541 ctggttgtct attggcatga cgctagtagt gtatactcat ttactttctg tcgctatggt
      601 ttctgtattt attggaggaa cgttgtttct ttctttttat ttttgggacc aaaaaatcgc
      661 tagactctta agtcttttaa aagctaccgt cctcaccttt tttttaagtg cgggattctt
      721 aattcctttt attcaacaat ctagagcaca agaattgaaa gtcccactgg gaaaagaatt
      781 aagtggtatg gctcctagtg atatgctgac acatatttta aataataact ataacaacta
      841 tactattgga ttattcttat ttctcggatt gattggtgct tttattttta taaaaaaatt
      901 aactgctgat gatttattta ttttctttct tggtgtattt gttctttttt gttcaaccaa
      961 tttatttcct tggcaacttt ttaaccatac accagttaag agtctacaat ttgtctggcg
     1021 gttaaatggt ttttcttctt tatttatagc ctatacgatg agtatagtga tctattatat
     1081 tttttctact aaaaataatt catggaaaat aatggtcttt acgtttcttg cactaatatt
     1141 gcatctctca ggagtaagca attctatcca tgctaacaaa gattcattaa cacttatagc
     1201 acctgaagac gctgttgcta tagctgaaaa ttacaatcat actgattatg caaacaaaga
     1261 gtcaatctat catcctgata tgattaataa tgatcaatat ttactaggta accaggaaat
     1321 aaatccaaca actcatttta tgagtaacaa acttacaatt gaattagata attcgaataa
     1381 taaaaatact gtcttgacga cacctattta tcgttataaa gggcaagtgg cttctattaa
     1441 tggcaaacta gtacaaacaa aattatctaa gtttggcaca actgaattaa ctattcctcc
     1501 aggtataaat aaagtcgtta ttacttatca gtacacaaaa ctagcaattg cttcaagata
     1561 tttatctatc gttactttga ttttattttt actttatcga tttacttttt ctaaacaaaa
     1621 acaaccgaga agagaagtac aacattctca ctagattttt atttaagaaa gactttttag
     1681 ctgtacacat aaacaggcta aaaagtcttt ttttatattt ctttaatttg cttcatacat
     1741 tatttaacat cgatatttat actgtgaaac atgcgtttca tccattatca aagtattttt
     1801 atcgataata aacttcatcc aaaaacagac cttgtgcagg gacggtttct cctgcatgtt
     1861 gccgcacttt gctttcaaat atttcatcga ttgtttctat cggcatggca cctgtgccga
     1921 tttctagtaa tgtccccatg ataatccgca ccattttata taagaatccg tctccgacaa
     1981 aagtaaaatg aagcatttct ccctttcgtt cgatagataa ttcatcaatc gtacgcactg
     2041 tggatttttt tgattttttc aatgcagaaa aaccaatgaa atcatgtgta cccactaatt
     2101 tttcgcaagc tgcattcagc ttgtccatat ccaactcttg agggaaataa aagctgtggt
     2161 ttcgctcaaa tgctgaagga atcacgttgt tccaaacata atagctgtat tttttgccca
     2221 ccgcattata tcttgaatgg aagcgctcag gcatttcttc gattcttttg atgacaatat
     2281 cccgcggaag gtaacgattc ataaagtcga tcatttctgc cagtccaatt tctgtgtttg
     2341 ttttaaaatt tgctacttgt cctcgcgcat gggtgccagc atctgttctt cctgatccaa
     2401 taatttctat ctcttcattt gtcatctgat gcaatacttt ttcgattttt ccttgaatgg
     2461 ttttatcgtc atctccaagt ctttgccatc ctgaatatct ttttccgtca tattcgattg
     2521 tcaacttgat gtttctcata ttttagtggc tcctctgtct tccatttggt cttctctcta
     2581 cctttttaag attgtagcac aaaatatagc tccttgcttc tctaagacag aagctataca
     2641 agaaaactgt ggaaatatat atttcagaaa aacaaaaaac agccaccgga tgaagtatcc
     2701 gctgactgtt caatttgacg gctcaagttt tactgtttga gatgcaagat actcaacctg
     2761 gcaatgggca cataattaaa gaaaaaactg attctttggc aaaaacctat tttattcagt
     2821 aatggccaag aatttataga aggaattctt gccagtccat aaatcgccgc aaaaagcaaa
     2881 ctgatcacag acagtccaag gtataagtgg cttacgtcca tttgcccatt ttttcgatag
     2941 atcaagaaaa gaagaactgg aaacaataaa aaaccactgt aacctaaacc tgatccgata
     3001 ttatggaccc atgtcgagac attccacgaa gattcatggg tattgatact gaacaaacct
     3061 gtaaagatac aatcacctat accatagaac gcgatggctg ctgtaactag ggtagctaac
     3121 acacgagatg tactaacaaa tgcagcatac aatgcaggca atgacaggat gaaaaagatc
     3181 cctgaaacca cagaccaaat aagaaacgct tttcttacag gactggaaac ctctccaaat
     3241 acactgatca cgctcttcat ttgatctaat tcaggataat acaatcctaa tacgtacgga
     3301 gtcaaaaaat cactcagcac gcctaataat aaaaagaaaa aaccaaattt acgtaaaaaa
     3361 tccatgatac gcctccaata acaattacct atgcttttaa ttgttattat aaacgacttt
     3421 atcttgattt aacagaaacc tctatttaca cgaacatcta tataactcat taaaaactcg
     3481 aatcatttta aaaatactaa atatctaacg ttcaacgttc acttaatcca tctaaacaat
     3541 ctttcaattt gattttttcc ccacttcttt tacgcgcgcc tagctctcct attgcagtaa
     3601 acaacagatc acttgacgaa tttaaggcag tttccattga atcctggata acaccgataa
     3661 taaatccaat accgacgatt tgcatcgcga catcattaga gataccaaac aaactacatg
     3721 ctaatggaat caataacagt gaaccgcccg caacacctga agctccacaa gcagctaaag
     3781 cagacaaaat acataaaagg actttcaaaa caaaaggtgc ttcaatccct aaagaatgta
     3841 cagcagtcaa cgtcatcaag gaaatggtaa tcgcggcacc gcccatattg atcgtagcac
     3901 ctaacgggat cgatatggaa taagactctt ttgtcaattt caatctttca gccaacatca
     3961 tgtttatcgg gatatttgcg gcagaacttc gggtaaagaa agcgggaagt gcgctttctt
     4021 tcaaacaaaa gaaaatcaat ggatagggat tttctttaat aaacaaaaac gtgaggaatg
     4081 ggtacacaac taatgcgaca aacagcatcg taccgatcaa aacaagtaaa agctgcagat
     4141 actctgccaa tccagcgatt ccagtcgtag caattgattg ataaaccaat cctaaaatcc
     4201 ctatcggagc aaatgcaata atcacttgta cgacttgtga caacgctata gatatctgat
     4261 ccaccaactc ttttgtcgat tctgaacgtg ctcgaaaagc tagtcccaac aatacagccc
     4321 agaataagac gccaaggtaa ttcgcatcaa taagagattg aacaggattt tgtgtgacat
     4381 tcgtcaacat agtagataaa acactttcca aactttgcgg tgctttttca gatactgttt
     4441 cttgtaaagt aataggaaca gtaaaaattg aacttacaat aactgctaca agtgcagaaa
     4501 gcaatgttcc cactaaatat aagatcaaaa taggcttcac gaatgtctta ttcccaattt
     4561 tatgcttggc aattgacgcc atcgtcaaga aaaaaaccag aataggtgcg attgccttca
     4621 agcaactgac aaacaacgtt cccaatattg aaataaatgt ccatgtgggg aaaaagatac
     4681 caaacaatat tccgactaaa ataccaacaa caatacgttg aactaacgac atatccaaaa
     4741 acttctctaa caaatgacca ccctttcaca attttaacaa tcattgtatc attttatgca
     4801 aagaaactaa aggtataatt ttctaaaaat tctaacaata caaacaacca tttcttgcta
     4861 attttgtact ttttctgtct acacgttgtt ttttgagata ttctaagtta ggatgttatt
     4921 aatgggcaac ttatcaggaa acgttttctt atgtttttgt ccaatttttt tgtgaggtgt
     4981 ttattcttga aaaaaataaa gaaggagagt tatggcttcc tgacaatctt tgtcgcttgt
     5041 ataatgatgg ttttacagcg tatattgatt tcagcagtca ctattcctac atatgcaaca
     5101 aaccctggtc gattctacat ctatacattt ttacaaactt ccgttgttat tggtgcaaat
     5161 ttgatcccac tttacatggg ttaccaatat caaaaaatcg cccgtttaaa agttttacat
     5221 tatttaagtc gttttgcctt tatattccta atagccacac tagtttgcaa tatctttttt
     5281 tacgtacaag ctggcagctt gaatattcgt gattattggc ttatcctaac acctatcagt
     5341 cagaattact ttacttatgc tgtttcttgt actttgcttt tttgtagtat tccttataca
     5401 gttggatttt taaataaatt atcgaaaaca acaattaaat atctactatt atttttaaca
     5461 attggattag ttggctgctc tactctattt aataaagacc cgtttgcatt acagaatgga
     5521 cagagtattt tatggatttt ttatttattt ttaatgggtt atggtttgaa agaatggaac
     5581 tggaaagaaa aggtccgcta taagcttcct caagtattta tcagcttgat tgttttattc
     5641 ggtttgatta ttttgatgac acaaatttca ctcatcatca gaggaaatac agatacagcg
     5701 ctgaggtttt ctaaaccata ttctctcttc gcaatgtatt atactttctc ttcgttttta
     5761 tttttagaaa tgctatctga taaattaggt ataaaaggaa gaggaaattt tcttgctgta
     5821 tttttgattg tcactcaagt tatatgtaat ttccctttag tttcttatcg cgtatctaca
     5881 tttatgaaac gtgaatttcc aaactcaggg aaggcatggc tgataaatat cgggcagttt
     5941 tttatttgat atttattagc agtgtccctc ctagttatct ttttatttta tatacaaaaa
     6001 atccgtttca ttcatcgttt catcgaatac ttcgcttttg attcgattga acaattaact
     6061 cagcgttgga aaaaaagaat tcattggctt tttgttcata aaagttattt tttagttgca
     6121 gcattttttt actgctttac cttcgtgcag attttcgttt tagaaccaaa agaaggttgg
     6181 aaagaaacta tccaggttgc cttaaaaata tttactcagc gacaagcacc catgatcctg
     6241 acaatcatca ttattatggg atttttcttc ttactattgt tgatttccaa tcgtttttgg
     6301 tacgccttga ctgctacctt gatcattaac ttactgctca ctatttcgac agtaatcaaa
     6361 atggaaatgc gggaagagcc tgtctttcct tcagatttaa aaatgctgac cggattgtca
     6421 gaactattat cgatggttag cccagtctta ttaatcgtgg gcggattgat ccttctccta
     6481 ttattgatta ctagtattat tgtacaacgt agactacaaa aacagtattc cttgaagatt
     6541 cattggaaac gtcgatttat cagtatcgct gtattattag gatgtttttc gggtgtcttt
     6601 ttcattaatc acaaaaattc gccttctttc ttgctgttta atttatttaa agtaaacaag
     6661 actttcttta accagcaagg cgccgtaaaa gataacggac cagtcattca attcctaaac
     6721 aatatcgaca ttaaaatcat ggaagaacct gctggctatt cgaaagaaaa aattaatgga
     6781 ataatgaaaa agtacaacac aaaggcagat gaaatcaacc aaacaagaag tgactggtta
     6841 aacaatcaga cgatcgttct caatttaagt gaaagcttca gtgatcctgc acgtgtccct
     6901 aatctgacag tacctacaga ccctattcct tacatcaaaa gcattataac agaaggaaca
     6961 ggaggattga tgttatccgt cggttatggt ggcggaacag ctaacatgga atgggaagga
     7021 ctgacaggct tgagtatcag taacctgtct gcttcgttag ttacacctta tacccagcta
     7081 gtggagcgtc agaaaataag tccaaatatt accgatctat tcgatgaaaa aatagccatc
     7141 catcctttta cagctgctct ttatcgcaga aaacaagtgt ttgataaatt tggttttgac
     7201 aggttctatt atttagacag tccggataaa ttaacttata cggataaaat agaacaaagc
     7261 ccacgcatat cggatgattc agcatacaaa gaaaccttga aagctttaca aagcaatacg
     7321 gaaacgtcac aattcatcca attatccact atgcaaaacc atatgcctta tgatgattac
     7381 tatagtgaat tgaactacac tgctgaagga actgctgtca ttcctaatag aagaaaagaa
     7441 ttacaaactt atatgcaagg ccttcactat accgatacag cagtaaaaaa attcatccaa
     7501 gaaatcgaca aaatcgataa accgattacc tttgtttttt atggtgacca tttacccgca
     7561 atctatagtg ggaatgattc gaaaaaatat ggattagaac agcaccagac agattatttc
     7621 atttataaca acaagtatag tcgtgaacaa gcagctacgt taaataaaga agttgttgct
     7681 cctaataact ttggtgctat ggctcttgag catgcgaata tcaaagtaac tccttactat
     7741 gcgttgttga cagaagtatc taatcatcta cctgcattaa caattgatcc tacagaaagc
     7801 ttgtctaatc gttttaatgg aaaacaggtc tttgttacag aggatagtca aattatccaa
     7861 gaaaaaaatt taactgagaa acaaaaagaa attctagaag attatcgtct gatacaatat
     7921 gatcttgttg caggtaacca gtacagtgca acatgggcga cacaaaaaat aaaagactaa
     7981 caaataaact gagcaagggg ttgtgataaa agacatgtca caaccccttg tttatctcat
     8041 taatttgctt gtccataaac gatttgatta aagccatttt tcaattcatt attcgctgtc
     8101 tcgttgaagt aaagataatt atcgggattc ccctgatttt gttgtgtcac caatactttt
     8161 ggctgatttt gctgaaaccc gaatagatag acgtgctttt tcagatatgg ttctgtctct
     8221 gcatcggaat agacggctgt gatttgatag tctgcttgac ctgtcccact ttctgaccat
     8281 tctgcagaaa caggcactcc accaatagcc atcttccact ccccgttcaa aatagcttga
     8341 gggactttaa gaccatataa gtccacactc atttgattcg tataactttt gtattgttgt
     8401 cccattgttt ggccccattt ggtcataaat gcctctaatt gtgcggattt gtctgcattc
     8461 cacagtgttt gttcaacttg ttcgctgctt tctgaagagg aagatgaagc ctctacagaa
     8521 gaacttgaag tagatgtttg agttgtttca gtcgttttgg aagactctga cgaagtagag
     8581 gtatctgctg tttctgtact tgctgcagtt gacttgctct ctgtgctagt tgttgtagta
     8641 gttgcaactg ttgctttcgc tgtttctttt gttgattctt ttttcgattc attcccgttg
     8701 gtacaaccag ctaacaaaaa agctagacca attgttacta aaatcctttt tttcaattaa
     8761 aaaactcctt tcgattactc ccacttcttt attctatcaa ataagcaatg tgattttatt
     8821 gatttttcgt attttctcat caatctttca aatcttctaa aaactgagaa gcacccgctg
     8881 atgttgtctg taatacatca gcgggtgctt cttttatttt aatcttcgtg attttccaat
     8941 ttacctcacg ctttatattt gtatttgaag aaacatagac ttttagtcat catcgtcttc
     9001 ctcatccaaa tcttgtcgga cagtttttcc ttggagtctt tgtacttggt agatgatttc
     9061 tttattgaaa cttaaaagtt caacaagcaa ttgttgcaag ccatattttt cttctttttc
     9121 agctaatttg attgcccgtg tgataaagac attttggaaa tcaaaatctt ttgcaaagtt
     9181 ttccaacatt gttgaggctg gtaaatattt gttttgacca tgttcgtaaa tttttccata
     9241 acgcatgact tcttctaaag tagacggaac gatttcattt tcaatcacta actcatgaga
     9301 gattttattg aaccatctcg cttcttgatc agcatattct gaaaaaaggg tacttaattc
     9361 ttgcgaagat gtacccaaaa catacaattg tgcttggatc aatttcattt cttctaattt
     9421 aaaatttgct aatacatgcc ccaccatggc gccagcagtt ggtttatgat ggtcgatatc
     9481 tgcttgtttt gtttcttgaa caaatttttc ttccactata ttcattgatt tacgcctctt
     9541 ttacttttgc tgattggact tcataaccta atttttccac aactgcagct aattgttcag
     9601 ggtggacttc atctggatca aagtttgctt tgattttgct cgcattaaac aaaactttcg
     9661 tattgcttac cccaggctgt tgagaaaccg catgttcgat tttttgcata cagcttggac
     9721 aagctagtgt atctaattgc ataataattt ttttcatcgt agttcttcct tcctttttct
     9781 tgatagtctt attctagatt acagcttcca taaaaaaatt gattcttatc aagttttacg
     9841 atttttcttc tttgctttcg tgtccaaaat acaataaacg catcgcgtta aagataacga
     9901 tcaagatact agcttcgtga acgaacatcc cactagccat gtaaataaac ccggcaaata
     9961 agccgttgag taaaaataag acgacaccaa tggcaatcac tacattttca cgggtattta
    10021 aaacagtttt cttagccaag cggtaggcat gaaccaacga ttcaaaatta gattgcatca
    10081 agacaacgtc actcgtttcg atagcaacat ccgttcctga tcccatagca atcccgatat
    10141 cagccgcagc caaagaggga gaatcattga ttccatctcc aataaaagca acgcgcaatc
    10201 cttgttcttg gtattttttc acaaattgaa ccttttcgtc tggtaataat tcagcatgga
    10261 cttcatcaat gcctaacata tcagctacat aatcagcagt catctgatta tcacctgtca
    10321 gcatcactaa ctgttttgct cctgcttgtt tcaacttagc taattgttca gcgacttctg
    10381 gtcgaatcac atctgacaca cctatgattt gagtgatttt tttgtcgatt gctacaataa
    10441 tgaccgagct gcctgtttgt tgtaattgat gtaaatcttg tacttgcttt ggtgtgagag
    10501 aaagtttgtg agcggcaatc aatttggtgt tgcccgcaca aactgtatga ccatcaatct
    10561 cagctgtgat tccttgtccc ttgattgtct gattatcttt gactgctaat tgttgaaaat
    10621 caatgtcttt ctcctgagca taatggatca cggcgcgccc cagaggatga tcggataacg
    10681 tttctaatcg agccactaaa gctaagctgt ccccttcatc ctctgtatag ttcttgatag
    10741 tggatacagc agtagttcct ttggtcaatg taccagtttt atcaaagaca aaagtatcga
    10801 ttttggaaaa agtatccatt gcttcaccgc ctttgactaa gatgccccgt ttcgctccat
    10861 taccgattcc tgctacattc gaaacaggcg caccaatgac taaagcgcca ggacaaccaa
    10921 gaaccaaaac agtgattgct aaacgaaaat cacgactaat cagaccaaca accaacgcaa
    10981 taagtaaaac ggttggagta taatactgag aaaatcgatc aatgaatttt tccgcatggg
    11041 atttggtatc ttgtgcttct tctactaatt cgataatttt ggcaaaggtc gtatcatctc
    11101 cgaccttagt tgcttcaatc gtcacgtagc catctgctaa aatcgttcca gagaagactt
    11161 catctccact gattttgacg acttgtcgtg attcaccagt cactgcggct tcatccgcat
    11221 accctttccc gtcaatgact cgtccgtcca caggaatagc tccaccagtt tttactaaga
    11281 cgatatcgcc ttcttccacg tcatcgattt ccacttcttc agtcgttcca tcttcttgga
    11341 caacactagc agtagatggg gccatctcgg tcaaagcctt gattgactcc cgcgttttgg
    11401 tcaatgtttt ttgttccaga tagctaccaa acaaaaacaa gaatgtaacg atagctgact
    11461 ccttgtattc tccgatcata aaggcaccaa gcacagcaat agtaacgagt aaatcaattg
    11521 agataacttt gactttcact gcctgatacg catggatcat aataggtatg accgcaataa
    11581 tagaagctat ggctagagca atgttataga acaagacaaa accagtaaat tttccgataa
    11641 aaccaaaaat aatcaacaca gctgataagg cggtgatatg atttcgatgc tcttggatcc
    11701 acttttgtaa tttcatcttt ctccctcttt tcctttcatt cttacgagac taagtataga
    11761 tacttatcca tcataaaaaa ttgaccacta tcaagtttag tcaaaaaggc ggttttcttt
    11821 tggtagaata cactacaaaa aatagttagc aagaagactc actaaaagtg atcttgccaa
    11881 ctattgtcgt ttctatcgaa attttcattg aatgattttc ctgtacagca tacatcggag
    11941 atctatttct ttatctaaga tttttatctt gctaattttt tcctttagtc tttcaaatcc
    12001 acgtcgttca tagaattgat acgtacagcc actatctgta aaaaggaata tttcttttcc
    12061 tttctctctt ttttcaaatt ctgttagcaa cttggtaccg atgccttttg ttttcaaatc
    12121 aggatttgca gctaaaaaaa tgatctctcc atctggcgaa tgcttcttta aatatgctgc
    12181 aagcatttgt tcatttgctg tctcgtaagc tcctttactt tctttagcaa atatattttg
    12241 taaaaaatcg aatatcttaa catatatttg ctgtccaaaa gaacgatgtt tctttttttc
    12301 tccttttatc tccgccagca aaacacctgc caactcgtcc tctgcataca aagcaagaat
    12361 ttgagttgct cgattcaact ccatatacaa aaaatatctt ccgtaaagat tcagcagaag
    12421 ttcattttcc atatatctct caaagttcat tccttcaaca gcaaaataga tagctttttt
    12481 gtaatctttc ttttctaagg gtttaaaagt tagtttcatc atttcgcctc ctcaacttca
    12541 atgattgtgt tatacttaca ttataataga cacagtgtct actatctata atcaatcatc
    12601 tataagtttg tcgattaagc gacagctttg ctttgatatg tcggataagg agataggtaa
    12661 atgaaaaaga cagatagacg cgtcaaaaaa acagaaaaag ctttggctga aacactatcc
    12721 acattattag taaataaaaa gattcaagca atcactatac gcgaactaac agaaacggca
    12781 gatgtacatc gttcgacttt ttatacacat tataaggata tctatgatct atatgatcaa
    12841 ttagaaagca acttttttcg ggatctaaat gaaattctct cttatgatcc agcccatagc
    12901 tatgaggaac tatacactcg cttgattgac tatgtttacg caaatacttt tttatatcgc
    12961 ttatttatcc tcaaccatgg tgatatttcg tttcagaaga aagttaaaca agttattgaa
    13021 gaaaactatc tgaacatatg gctatatgaa gacaagaaaa cgattatcac agaagaaatg
    13081 agattcttta ctacgtacca cgtccaaggt tgtctatccg ttctcaatcg gtgggtagaa
    13141 agcaattaca agtactccaa aaaagaatta ttagaactct tacagaaact gaatgatcac
    13201 atagagaaaa tcatgccttg aataaaaaca tactaagctt agttaagaag aaccgtaacc
    13261 ttctcgtata acaactgaat cttatttagc caaattgttt actgaagatt ttttgagtaa
    13321 aatttctata attcctactt attgaaaagt agtttgtagt gctgcttctc cctctctaag
    13381 tgatatatct tgttgtacca atcaaattca cctcatcatt aataaattac ttttaggcta
    13441 atttattaca acttataggt tgaagctgta accaatttat agtcatatta tatttttatc
    13501 atccagttta ttgaattctt ctcgtttacc tcaattcatt ttcaagggga atcagctcat
    13561 catctaacat tttacgccat gctcgcttca tttcttgtgg gtatttaaat ggtttcgctt
    13621 ctggatgttt ggctttatat ccttcgtaat ccttactgtt gataattgct ttcatattag
    13681 gaattggatc ttcacctggc atatattgca gattagaagc atgtaatttt ttctcagaaa
    13741 caaaccactc attggaaaag acatgaatca aatcatcact tacttgtgta aaatagcgac
    13801 gtttaatgac aaagatatct tcattgctat ctagtgtacc ttcatttctt tgtgcttaaa
    13861 ttcaaagata attttctcaa taaagatttt tgattccagt aagattcctt tactcctttt
    13921 acaacatagc gttgtccttt gttttgaaac atcttcttcc cctctttgcc attaaaaata
    13981 gcccaattct ctataaagaa gattaagctt cattattata atataaagtt taaatttggt
    14041 ttattactta ctttttctta ttctctagta agctaatatt gaaaaaaatg taatcagatc
    14101 attattttta tatgttgtaa cttcctgata agaaaactaa ttaaaagaaa gaggtaacta
    14161 cataaactaa ttaaaaaagc taacttcata gttggaggat aaatggaaga aacttcaatg
    14221 attgtttctc cttgtgttcc ttcaatcgac actagtccta ttatcaatct ctcttttttg
    14281 tccatttact tttgcgactg ttccccaata atcaaataca ggaagcgtta ttttttgtcc
    14341 tttagattca tgtactgtat agactatttt cgaatccatc acttgataag gcatagtatg
    14401 ccattccttg ccaattctta cttcatgatt ctgcaaatgt tctttgtatt gtaatgcttc
    14461 cgtgggtgca tagtcataaa gtcctccggg aaggagtgtc gtgtaacgaa taagattttc
    14521 ttgattcatt accaagtgat ctctctgagt gacaacttta tgttcatagt ttgcttttgt
    14581 tgcagcagat acagccaaca gtaataaaac aattgccgtt atattaacta cttttctttg
    14641 cttagctgat gtgctcttca tgaaaacgaa cgataaaatc aacgagccaa acagtacttg
    14701 aaagcctaag attcgccaag gaaattgaat catttgaatg ggtgtatttt gtaataaaga
    14761 ccatggaaaa aatttggtgg ataagagcca agagactacc gcactaaggt aaatcacttt
    14821 ttctttgcga ttaaatcctt taaaaaagaa gagtggacag atcaaagaaa taataccaat
    14881 gattcctacc gtaaaggttg tcaggtcatt attcatcgca ccaatcaaac tatccgagac
    14941 atttaatgct tggtgttgta aggtcgtttt atctggctca ttaatcgatt gaaccatgat
    15001 ttgtcggaac atcggatacc aaaagtatgc tgttaaaaga atcgtcattc ctactgcctt
    15061 tgacaactcc acaatcaacc ggatgctttc tttttttctt gtttcactta ctacaaagcc
    15121 gcctaagact agtaagccaa cggctattac actcataaaa gtggaaagaa catgtgtata
    15181 aatgagtaag ctcatactga ctgctaatgg aattgcacat ggctttttct cataaatcag
    15241 tgcgtaagca taatacataa ctatcggtat aaccgtaagt gcaatcgctt cagcaatagc
    15301 agaacgatga taaatatcaa tcgtacggta cccataaaat gtatataaac agctaaatat
    15361 aaaagccgca tcgattcttt ttaaaaattt tagtccataa tgaaacatca gcatgatagt
    15421 acaaacattc aatagccaaa catataagat ataactaacg attaaattat tacttatcca
    15481 ataaaaaatg actgccggaa aaaaggtcaa gtagggataa aaataattca ctccgctccc
    15541 ataatgatta aacgtcgtaa aattgattgg accagaaaaa attgaagata accctttgac
    15601 tctagcaaaa tggaaggcaa tatcccccga tgtagaaata gtatgaacat ttctaaaata
    15661 attgattgaa taaagtccag caataattgc taggagaatg gacaatatta tttttttctt
    15721 tacttgcatt acttaattat atactccttt catggattgc tttatctagt ataacttaaa
    15781 ttttaaataa atcgttattt atttttactc tcacttaaaa aagcactaaa attataagag
    15841 agggaggtaa tggtctgaac accaagaatt gttccaacat cgaattgctt aactaatgag
    15901 ttcaaagctt aattatgtgg ctcttatatt taccgagcgt ggattgcgat ctctttagcg
    15961 aaacaaacat ctttcataaa aaaaacagcc atatccgctt tcttggatat gactatttac
    16021 tactacttta ctatctttca ttcttcttgt ctcacttatg ataaggcgct gcgtatcatg
    16081 agtgaatgat attaggaaaa cttcataaaa taaatctttt atacatttcc acttctagat
    16141 ggtcattcat gttagaagaa tcaaactatt tttttactgt cgtaataata ttaaaacact
    16201 agaacaaatc aattaaattc cataaaatat aaataataga cctagcatca tctggtataa
    16261 aatggatgct aggtccgtta tatttactca aaattgattc tacgaataga tcttcttatc
    16321 atttctattt tagtagtcat gcgttgttgt tcttctaaag gtggtaatgg tataagtagt
    16381 ttagataaat ttgtaccatt tacatttggt tgcccagttc cgtaagaaat tttttctaat
    16441 aacttccaat ataatggcga gtctaagaag cagtctacgt attctgataa taatttctca
    16501 actaacctaa ttcgaatcaa gtaagaagca aaaacagatt cctcacttat ttccttaacc
    16561 aagaaacttt ttcccatagt tcctcctgtc cttgctatca aaatgtcatt ttcttccaat
    16621 cttaaatcaa ctagtttaga attactaata tcacagtaag gcacactgga ccaatttaca
    16681 cgtccctctt gtatatctgt gattctcaaa aacttaacat tgccttgttt tttcgccgaa
    16741 tctgtatagc catattgaat cttagtagaa atacttccta aataaaccca tagtcgaaca
    16801 tcgctagatg atttttcttg tcgttcttta tgaacaacag tgatcatctt gagttgtccg
    16861 actatttttt agttgcagca atctttttgg gacgcttatt tgcatgaagc aacgttgcta
    16921 agtctagtaa taaaacattg gctaacacag gattggcttt ctcgcgatac gctgcggcgc
    16981 cactttcaaa caatactgct agacgatctt ctacaacaac gatttgttcc aaatacggtg
    17041 gaaatttgat tcgttttagg atccggctag gagagtcccg ctcggacact aagagttccg
    17101 aggtataagg accataagaa cgactggcta agagataatg gccataaaaa gccacacctt
    17161 gaatcttatc agatgttttt ctgctcattg tcacggttgg ttggattttg tctttgcggc
    17221 gaattgctgt tttcatttca gctatcgtcg gcaatagata gaaagtcaac tgtccttcac
    17281 cttttagtgt gaagttgcca gataccagtt gatcattgtt ttgagtcaaa tacgatgctt
    17341 gtggaatctc tgagagatca gttgtcagcc aataagcgat tggctgtccc gtctcttcag
    17401 atgtatctgc taagatatcg atcaaacgta aagcggctac tcgtccagta gcagatcctg
    17461 tgattgtcaa ccataaaagt tcccgttttg tatcatatac taagccccct acatgtggcc
    17521 gtttaggtaa aatcagcagt ttgatcggct gtcctgtttc tctatccagt acatgaataa
    17581 tagaatgatg ctgatggtca tagcagtagg cagaaataaa cacaaaatct tgagaaaaag
    17641 ccaacccttg cggcgtcatc cattgacaaa cctcattttc gccggtttcc ggcaccacca
    17701 ttcttgtttc tgttaatcca ggaatgttat attgttttgc ttcagccttt tcccaagctg
    17761 cttgaacaat aggataccgt tttaactttt tttcaatatc ctgatgtatc cgttgctgct
    17821 tccaatgctt aaactttagg acatagtaaa caagaaggcc aatgaccagc aacactaaaa
    17881 tcattattgc tacccgctgt gtcatttttt tgcctccttg ttctattcga tcaatcgtcc
    17941 atataccgct attaattacc gtaactacat gtttctttta ctagcgtttc tttataggat
    18001 ctttcataat ctcatttata gtaacattaa gaatccctta ttctactata gtcacttcca
    18061 catcaatatt gcctcttgtt gctttagagt atgggcaaaa tgcgtgggct tcttctgcga
    18121 ttttctctgc ttggtccttc tcaagtcctg gcaaagaaac ttctaaaacg accccgattt
    18181 ggtagccccc atcttctaat tgaaataaag atacccgtgc tttgacttca gaatcagact
    18241 caagattatt ttcttccagc atgtgttgaa ccgcgccgtt gaagcaagat gcgtatccag
    18301 ccgcaaaaag ttgttccgga tttgtccctt cactatggat tcctgggcgg tcgatcttca
    18361 tgtgcatact tccgtttggt gcctcaactt cgccatcacg gcctccacga ttgatcattg
    18421 ctgtttcgta catttttttc attgagatca tcctttcttt cgtcgttcta tattaagtgt
    18481 aacgagtatt ttttaatctg acaaaggtaa tgcttgcatt cggcgacatc agttagacaa
    18541 catcgatgat cagtgataga agcagtttat ctatacaaaa aaagaagggt tctatgatat
    18601 ttggtgtcct tactcgcctt gagtaacaac accaaatatc atagaaccac ttaatcagct
    18661 tcacactgct ttttctactt tcgtagttga aaaaaactta aacaaatcat cggcaaatag
    18721 ttaaagaaaa aacttaatcg ttgaaagaag cccaattgct taaagaaggc aaactgacta
    18781 atcgacggaa tccgtgctaa tccgtaaaga cctgcaatta gtaaactaat aactgttaaa
    18841 acaagataaa aatggctaag cgtaccttga ccactttgac gataaagcag aacaagtaac
    18901 aatgggaaaa gtagaaagcc tgcatagcct agcccagaac ctatattatg gatccaagtc
    18961 gatagattac aagagctttc attcgtattg atactgaata agccagtaaa aatacaatca
    19021 cctatcccat ataaaccaac tgttgcaaca acaagtattg caagtgtttt tgatgttcca
    19081 acaaataggt gatacaacgc aggcaacgaa agaacaaaaa aaagtccaga tacaactgac
    19141 cagaccaaga aagcacgtct gactggacta tctacgtcac caaacacact aattacggta
    19201 gtcatttgat tcagtttggg ataaaaaagg ccaaggatat acggtgtcga taaatcactc
    19261 atcacaccta ataataaaaa ataaaaacca tatttttgca aaaaaatcat tttcttcccc
    19321 tactttttag tgattattcg atacatccaa caatcaaatt ttttctcatt ttaacacata
    19381 tttttattct agttgtcttt tgaacaatca ataacataaa ggtgagtttc ttctacattt
    19441 taaaaaatcg aatggcactg aagagttcag ttgtcagcat caatctccta ctcaaaaagg
    19501 caaaaaaacg taagtcagat gttgttgaca tctcacttac gcatccattg attgaaaatt
    19561 tcttagtaaa atctactagc ctaaaaattt gaagacttct tctcgatcat acagtttaaa
    19621 tactttctcg cataaaaatt gagtaattag tgccgcaaaa acagggatga ctaaccaaca
    19681 aagaacaaca agaaagccat ttagccctgc atctaaagaa gccaacggac caacaagtcc
    19741 gaccacgccg aaaccagcag aagcaggcgt acctgaaata gaaaatagag cgacaggaat
    19801 agctgtaatc actgcactaa ctaaacaagg aataagaatc acaggttttc taaatagatt
    19861 aggcatcatc atcttcatag cgcctaaagc aatcgcaatt gtcacacctg acttattaac
    19921 cttccaagag ttcactacta aaacaaaagt ggttgcagct acgcccatcg cagccgcacc
    19981 agcagaaaca ccatctagtt ggattgccat tccgattgct actgttgaaa taggagagat
    20041 aatcaaaaag gcaaaggaac aaccaatcaa gatagacatt aacagcggtt gtaaggtagt
    20101 gaagttatta atcaatgatc caattgcaga agtaattgct gttacaaaag ggaataaata
    20161 aaaaccaagt aaacctgcac ctacacctac aacaatcggt gttaacacaa tcgacactga
    20221 cccaaaacta tttccaatca gtaaaatcag cccgacagca atcgatgctg tcagcatcgt
    20281 attaataata tcgcctgtac cagcaccaac gtaaacaccc gtttcaccca aatcaggtgt
    20341 gaatttgact acaccagaac caacaaaaga agctccggct acaaccatca ttctcatcgg
    20401 atcaaatcca aattgtaagg caatcaaacc accaatgatc aaaggtgttg ctagttgaaa
    20461 aataactgct aactgtgcga tcgttaccgc aaagggatac gcactaaaat attttaaaat
    20521 ggcacctaat actgcattag gaatcaatcc aataatgatt cctgttgctg taccagcgag
    20581 gactttattc agaaaaattt tcggtgatat tttttgtgaa gtttccattc aatctctcct
    20641 ttattttacg ttcaaactat cttcttttgc atggacaagc tcagtcaaga aatcacaata
    20701 aggcgtcgca acaccatatt tcttcccttt tctagaaatt gcgccattga tataatcgat
    20761 ttctgttaat cgatggttct taatcaagtc ttgatacatt gaaggataat gtaacccaat
    20821 tgtttccggg tcaaaacaag attcacaatg tgcaatgact tctgggacat ctagttcaat
    20881 cttctctact gctgctactt tggcaaactc attgacaatc gtcgccacca ttttatgagc
    20941 agtcgatgtt tttcctagct cagccatatt aacgtctaag attgtgcata gtccgttcat
    21001 tgttccgttc acacatgctt tgcgataaat agaatagtga atattgtcag aaaaatgagc
    21061 atttaatcct gattcagaca atttgtctgc taattttttt gcagctgctt cttttccatc
    21121 acctaaattt tgtaattcta ctgaaccact tccaaataat ttgacctgac caggaccttc
    21181 aagacctgcc gtccacatgg tatttccaat atagatattt tccattggta caaatttctc
    21241 aataatatct tcatgcccga taccatttaa tagacataag acctctgtat cttttttgat
    21301 taatgattgg atatcctgca gcattttttc cagctgcatc gctttggtaa atagaataat
    21361 cagatcaact tgatcttctt tttctacttc ggattgaaga acgattggta gttttgcttc
    21421 tacctcttta ccattaaaat ttgcttgtaa tccatgttct ttgatttgtt ggacatgttc
    21481 tgcccagcca tcgattaata atacttcgtt cccactttgg tgaagcatca acccaaagcg
    21541 acttcccatt gcgccagcgc ctgcaattgc tattttcatt gattcttcct cctttttctt
    21601 tctattatac tcatttgaga atcttttcac aacctcgttt tgttattctt ctcttattta
    21661 tcatgtttat atccaccatt caagttattt tatcagcaaa tcaataaaaa ctggaataga
    21721 atcccaattt catcgtaaga gaaactagac tagcaataag aaaatagaaa cattcataga
    21781 aaaaatctca ctcctctgat tataaaagag ggggcattaa cctcgtgtat actaaaacct
    21841 tccattcatt cgatggatga ttttcttttt gtgaaaaaat attcattgta gtacgatgtt
    21901 gcgcaacaca gacggatggt attgcttatt atttattaaa atcgaacaat acacttattt
    21961 aaaaacttct ttcccagatt ttcttccatt tttacagtta taactagtat tatcatgaaa
    22021 aatagccttt tattgtctat gggaataata atttaaaatt ggtacttttt tattaattag
    22081 ttcatcgccc aataatctct ccagtttctt agcatctcct gactcaaccg gaattatcca
    22141 agcccagtct aaataatttt ttaaaatctc atcatatttt gcaaatattt cattgccata
    22201 ttccgtcctt aaaattatat tttcagtgcc agacaagtga tttatattga gttgataaaa
    22261 aagatctcca tctaaaaaaa tatcattaga agaagatttc cccacagtaa tgaccattcc
    22321 atcagctcta tgtgttacat acaaataatt gtatgacctc atttcaccta atgaatcaat
    22381 tatttcttgc cctttagctc taatatattg atctctatta cctatctcac tcttatttaa
    22441 caaaagtttt ttcatgtcaa atttaatcgg ctgttttatt tttgtaaatt cgcttctact
    22501 actaatccaa tccgagatta atttttcaac atgtcccaga gtaaactctg atttattcgc
    22561 taaatacttt ttttgttgta ttaaagacat ctttccccga agctcaaaat tgagcctaca
    22621 tcgattctct atctcttcaa aaagcatctt atcttcatca ttcttatatt catcattcat
    22681 aaaaccaatc cagctcctcg attaagataa tattttaatc tcatacaaaa actagctctc
    22741 atcaaaacaa ccattgtttt gatttctcat gaaatttctt tatacttttc tccgattata
    22801 aattcctaac cccttgagaa aattacgcat atatttatct ccgcaaggct taaaatttcg
    22861 ttggccttct tttctcaaaa atgccccgat ttcgcctttt gatggataga tttctgcctc
    22921 agcaaaaacg tctaaaatat catcagtggt catagacaag gcgattttta atttttttag
    22981 caacacattg ttgatatatt tgatttcctt gcttttttgc ggcgtgtcta ctatcggctt
    23041 atctttttgc ggaccacgcg ccaagagaat caggccattt aggaatgcgt caaaaacttg
    23101 gttgttgatg gttttttcat acacattatt ttctgcatta tctttgttct tatcctgtac
    23161 tttggtcagt accgcctgag cttcttcttt agtcagtggt acaccgccta gctcaaaagc
    23221 tttaatcaaa tccgtatctt tcaaatcaat cgcataacgc aatcgcaata acctatcatt
    23281 gttattcatt tgttcctccg aagtatttag tctttacttt cttacgtact ttttgtcttg
    23341 ctaagatttt taggatcaat gataaaaacc gcatcactta tgataaggcg ctgggtatca
    23401 tgagtgaatg atattgctga atacaaatag tccgaccaca ttaagttgat cggacttcat
    23461 aagcatttta ttgactaata cttttttgct taagaattga ttctaacatc ttttgctaaa
    23521 gttaagcatc ctatagttcc tgcattatct gctaacttag gtgtgacaat gtattcttcg
    23581 atatttggcg tatcgacata gccgttaatt agttctaaaa aacgtctttg tactttcttt
    23641 ttcagatgtt cttgttgcat gacaccacca ccaaaaatta taatatctgg tgaaagcatc
    23701 agggtcgtat tacaggcaca ttgtgcaata taatccgcct cgatttccca gaacggatct
    23761 tctatagaaa gatcttgtcc cttggttccc gtacgcatct cgatagcggg tccagatgcc
    23821 aacccttcga gacaatcatg atgaaaagga caagtaccag agaaagtatc tcctgaatgt
    23881 ctcttaacta acgtatgccc catttctgga tggctaaacc cttcaacaaa aatcccatct
    23941 tgtatagctc ctccaccaat tccagttcca acagtgtaat aaacaacact ggataaacct
    24001 tttccatgac ctgaaacata ctccccgtaa caagcagcat tcacatcggt tgtccaggca
    24061 attggaattt cgaaatgctt attcaacgtt ccaacaaaat caaaattttt ccaggcagtt
    24121 tttggagtag aggtgatatg tccataggtc tttgattcac gatggatatc aatcggtcca
    24181 aatgacccta ctccaatgct ttctaattgt tctttatagt gattaaagaa atcaattacc
    24241 aaactcattg tttcattcgg tgtagttgta ggaaaactca ctctttctaa aacctttaaa
    24301 tcatcattgc caatggcaca aacaaattta gtcccacctg cttctattga gccatacatt
    24361 tcttactcca ccgtcctttt atctaggaat tcgttcttcc ctgtctaata gtttttcgaa
    24421 aaactcatgt ggttcctttt ggtaccaata ggcaacagaa gaaatatcgt cttgacgctc
    24481 ataaagcttg atgtcatcat ttcctagggt ttggaaagta acacgtaaat tttctttgaa
    24541 actaatgggg tcgggtaaat gccagcggta caacccatgc atcggtaaag catcatcgcc
    24601 aaaaccatga actggatttg gattaggttt tccagcacta aaataatccc gggtgtgatc
    24661 tcttttagat tgaaaaggat agcccataaa tagcgtatta aaattttgag catatgccct
    24721 ttctccaaag ttctttttat ggaaagccca agcgccgcca aaataatctt ctgctcctgt
    24781 agatgttatt gtagggaaat cttcatctcc atctatataa aacttaaatt ctccttcccc
    24841 ccaccagtat ctttccaatg cgcacaaagc aaagtaggtt cctacataat acccagtgcc
    24901 tttgattcca tcaataaggg tgtaatcttt tccttttgtc gtgatgggtt cacggttcca
    24961 tttagcatga aaatacatcg catcatccgg tacttcagtc aatgcataat taaatgtata
    25021 aaaaatgtgc ttaataaact caggatgttc attcgttaaa gtaatcttag ctttttttcg
    25081 aaatggcatc tcaaaatagc aattcatccc tgatgttgga ttcactacga ttggtaagga
    25141 attcacttcg catcttgtcc caaacccatt acaaaaaaaa tctcctaatg gactttctac
    25201 agaaggagtg gattcatcat cccaataaat acgcaaaatt aaatcacgta agacgaaact
    25261 cccagcgatt gttttgtctg tcactgtcat ccaaaaatga cgtaaaattc cagggccttc
    25321 catttctgct aaagtaattg tctctccagc atttatcgga acagaaggtg acccttttct
    25381 cccaattcct agatgactcg ctgtcattgc cgctttccct ttttcaccat ttttattttc
    25441 tggtgaaata gttctacttt cctctccctt gtggagccat atattattta aacaattcat
    25501 tttattcgtc ctctatcctt tcttcacttt tttgattatt catatagatg atttgttgtt
    25561 gtgcctccgc aataattgct ccaatatcta aattatcatt tcctaactgc aaagcgattg
    25621 acattgccaa gggaagattc attcctgcaa ttacgtgcac tctgtcagaa atataggtaa
    25681 aaaacttttg atttacactt cctccagcca tgtcagttaa aacaattatt tcgtctgtag
    25741 tatccactaa ctctttaatt tgttcatcta tatcatcaat ttcttcgaca tatgcacata
    25801 agctactaat ttgattatgc atacctgtta aaaactctag tgtctcttta aaaccctcag
    25861 ctagtattga atggctagca atgatcaatc ttctcataat tcaaatcctt ttcctttacg
    25921 ctaatattcc aaatgcagcc ataaccatcg caaaaacaat aacgagtaga atcaaaatgg
    25981 tcatactgat ttttttctta ctgagtagat aatatgctgc agcagtaata attactggaa
    26041 ttaatgttgg taaaattttg tccaacattg gttggatttt catactaact tcaccaaaag
    26101 tgaatttaag tggcgttgaa acactgataa ctgaagcaat taatgatcca actactgtta
    26161 gccccaatac tgaagcagct tcagttaaat aattaatctt ttcaccgtat tcagtaacaa
    26221 tcttcattcc ctgcttataa ccaaattcaa agcctctcat tcggaaaaag aagattacca
    26281 tgattaaaat gaaccataaa aagattccta caggattatt ttctaaagcc atataagccg
    26341 caattgatcc catgatggtt gggatcatcg tcatcaaaag agagtcccca ataccagcaa
    26401 ttggacccat cgtaccaact ttaaagtcat taatcgcatc ttttgcttct aatccttgct
    26461 tttcctccat tgcaacacaa gcacctaaaa taatggctgc cagccaaggc tgtgtattgt
    26521 aatactgaaa gtgattattt aaagcttcga tgtattcatc atcatctttg tataattttc
    26581 ttaaaatagg tgacaatgcg taagtcacag atgcaccttg ctgtgtttga taattaaagg
    26641 tacaaactga catcaaccaa cgatagttcg ctttcctaag atcctttttc gttacttgat
    26701 atttttcgct cattttattc atcctcccca ataaacccaa catcagaagc tcccgcagaa
    26761 gcagtgattt gtgcatcttt ttcagctcta tgatacacat taaatgctaa agcgaatcct
    26821 atgatcgccg ctccaataat aggtaccttc aaaaaagcgg ctaagaaaaa tccaacaagt
    26881 aaataactaa tgtatcgacc tgtaggcata aagcgtaaca acatcgcaat accaacagca
    26941 ggcaacattt ttccggctaa tgttagccca tccataaacc aattaggtaa aacatccaaa
    27001 acgtaattaa cagcagactg tcctaaagta acggcaataa atactggaat tgctgcagat
    27061 aaaccaaaga aaattggccc caaccacaaa acactattca ttttcttaaa ttctcgttta
    27121 ttcgcatatt cctgtgattt gcgaacaaca aaaccattta agattttgac aattacatct
    27181 agttgcaccc ctaacatacc tacaggtagt cctattgcta atccggcttc tactccaagc
    27241 tggctaccaa atgccgttgc aataattgcg cacgttccat agtccggtac agatgagcca
    27301 ccaaaaccgg caactcctaa tgacatcaat tgaactgtac cgccaactat caaacctgtt
    27361 tttatatcac ccatcaaaat tcctgtgaat aatccccaaa aaactgcgta atttacaaag
    27421 atcatcggaa tcccataata atcaacatgt ttaataaatg ctaatatagt cagcataata
    27481 atttgaaaaa tagtaaaatg cattatcctt actcctttat cctaattttg attgaatttc
    27541 ctgtgcctta tccgccggaa caaattgtgt aacaatttct acaccagaat ccaacaattt
    27601 tttatacgtg ctgatatttt catctgatgc ataggctcta gaagatagct gtaatgcatt
    27661 atctttaaca acagtaccgc caacaattag tcggtcaatt ctaataccgt tatctactaa
    27721 atgaaggatt gtttccggct ctttcacgat gacaaatact ttttcctgat cgtatttccc
    27781 ctttttaaaa ttcgcaatcg ctgtttcttc agtgataatt gatacagcca ttccagcagg
    27841 tctagctaaa cgcatacttg atttttggat gtcgttattt gcgacagaat catcgatcac
    27901 catcactctg tttgctttag acattggtgc ccattgcgtg acaataatcc catgcaataa
    27961 acggttatca actctcgaca taacaatact cataaatact tcctccttta tctttcattt
    28021 tcttacaagc aattaccatg ccaaaacaaa aagaacttga taaatcaagc tctttttaca
    28081 taatcataaa cgtatttaac ctcatgaatc gggatgtcga cactataata atgttcaacg
    28141 acactgaaag attgcttaaa atgtgtctga aatttttgaa attcctcact cgtttcattg
    28201 aaataaattt catcttccaa agtattttgt tttactaatc gttcaattaa gcaactaatg
    28261 tgaacatata atccgaaaca cgtattatta tcaaatgaaa ttcctagatc attttgtaag
    28321 atgtatatag catctgaaac ccgttctaat aatttagtcg gatttaatat ggtcaattgc
    28381 tccattaaat tcgttagact aaaattcttc agaatattat t
//
LOCUS       DAZUMB010000023        28326 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000023 DAZUMB010000000
VERSION     DAZUMB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.023"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..737)
                     /locus_tag="ACG80L_000855"
     CDS             complement(27..737)
                     /locus_tag="ACG80L_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS5023131.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(755..1252)
                     /locus_tag="ACG80L_000856"
     CDS             complement(755..1252)
                     /locus_tag="ACG80L_000856"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5023132.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(1289..1741)
                     /locus_tag="ACG80L_000857"
     CDS             complement(1289..1741)
                     /locus_tag="ACG80L_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5023133.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(1876..2481)
                     /locus_tag="ACG80L_000858"
     CDS             complement(1876..2481)
                     /locus_tag="ACG80L_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5023134.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(2931..3551)
                     /locus_tag="ACG80L_000859"
     CDS             complement(2931..3551)
                     /locus_tag="ACG80L_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5023135.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(3553..4422)
                     /locus_tag="ACG80L_000860"
     CDS             complement(3553..4422)
                     /locus_tag="ACG80L_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5023136.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(4445..4852)
                     /locus_tag="ACG80L_000861"
     CDS             complement(4445..4852)
                     /locus_tag="ACG80L_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5023137.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(4871..6160)
                     /locus_tag="ACG80L_000862"
     CDS             complement(4871..6160)
                     /locus_tag="ACG80L_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5023138.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(6187..6489)
                     /locus_tag="ACG80L_000863"
     CDS             complement(6187..6489)
                     /locus_tag="ACG80L_000863"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5023139.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(6514..8607)
                     /locus_tag="ACG80L_000864"
     CDS             complement(6514..8607)
                     /locus_tag="ACG80L_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5023140.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(8588..8722)
                     /locus_tag="ACG80L_000865"
     CDS             complement(8588..8722)
                     /locus_tag="ACG80L_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023141.1"
                     /translation="MAYFLFALLTFFPLQVAEISTFYYVSGTIYLKSEGDHFYENKNN
                     "
     gene            complement(9096..9860)
                     /locus_tag="ACG80L_000866"
     CDS             complement(9096..9860)
                     /locus_tag="ACG80L_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS5023142.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(9971..10840)
                     /gene="mdcB"
                     /locus_tag="ACG80L_000867"
     CDS             complement(9971..10840)
                     /gene="mdcB"
                     /locus_tag="ACG80L_000867"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS5023143.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(10865..11941)
                     /locus_tag="ACG80L_000868"
     CDS             complement(10865..11941)
                     /locus_tag="ACG80L_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5023144.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(11965..12558)
                     /locus_tag="ACG80L_000869"
     CDS             complement(11965..12558)
                     /locus_tag="ACG80L_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS5023145.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(12562..14238)
                     /gene="mdcD"
                     /locus_tag="ACG80L_000870"
     CDS             complement(12562..14238)
                     /gene="mdcD"
                     /locus_tag="ACG80L_000870"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS5023146.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(14228..14527)
                     /locus_tag="ACG80L_000871"
     CDS             complement(14228..14527)
                     /locus_tag="ACG80L_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS5023147.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(14629..16275)
                     /gene="mdcA"
                     /locus_tag="ACG80L_000872"
     CDS             complement(14629..16275)
                     /gene="mdcA"
                     /locus_tag="ACG80L_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS5023148.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(16282..17193)
                     /locus_tag="ACG80L_000873"
     CDS             complement(16282..17193)
                     /locus_tag="ACG80L_000873"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5023149.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            17313..18170
                     /locus_tag="ACG80L_000874"
     CDS             17313..18170
                     /locus_tag="ACG80L_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5023150.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(18357..18719)
                     /locus_tag="ACG80L_000875"
     CDS             complement(18357..18719)
                     /locus_tag="ACG80L_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS5023151.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(18908..19090)
                     /locus_tag="ACG80L_000876"
     CDS             complement(18908..19090)
                     /locus_tag="ACG80L_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023152.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            19065..20504
                     /locus_tag="ACG80L_000877"
     CDS             19065..20504
                     /locus_tag="ACG80L_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5023153.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(20511..23063)
                     /locus_tag="ACG80L_000878"
     CDS             complement(20511..23063)
                     /locus_tag="ACG80L_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS5023154.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(23177..24508)
                     /locus_tag="ACG80L_000879"
     CDS             complement(23177..24508)
                     /locus_tag="ACG80L_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5023155.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(24474..25952)
                     /locus_tag="ACG80L_000880"
     CDS             complement(24474..25952)
                     /locus_tag="ACG80L_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5023156.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(26257..28092)
                     /gene="tdc"
                     /locus_tag="ACG80L_000881"
     CDS             complement(26257..28092)
                     /gene="tdc"
                     /locus_tag="ACG80L_000881"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5023157.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         8141 a         6026 c         4849 g         9310 t
ORIGIN      
        1 atgccgctct tcttatttcg attcttttat aattctgctt ctgattcatc tttggttagt
       61 gtgtgtctaa cactagttac gccaaatgtt tgatcgattt tttccttcaa cgcttcgatt
      121 gcctgtgcgc cacgtgccgc ttgttcagca ttgatggatt caatgaccag caccgctgct
      181 tgtgtttctt ccgtcagcat tgttctttgg gcttcacgcc agttgagaca gcgacagatc
      241 gctccttgtt catcatagta gatgatttct tccggaagtg ccggagcatc ggaatctgca
      301 cctaaaggca ggaatgattc gccaccttgg gcttttccta aatgcagatt tcctgcgatc
      361 ttttcgatat cttctccgcc gcaaggtacg ccaaaagaca aggaaatact attatagata
      421 tctacaagcg gattgattgg gaaaaattcc cgttcttgat ttgcacgttt cagtaaagct
      481 tcaatagaag aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg acgccactga
      541 gcaatgactt cgttttggct gaatgtttct tctgtaaggt aatttcttgc ttcttttgct
      601 gcctcattca ataattgatg atacgtttct tcactttctt ttgttacgtg gttatcgata
      661 ccatttacgg ataagatttc gatggttgct tcaggaaata cttcccagaa attggtatcc
      721 actattactt ttttcatctg ttcacacctc ttgattagaa taattgcttg atgaatagat
      781 agtctgtttg gggatcatct cccatataaa aaacatgttc gctgtatttg ataaatcctt
      841 ttttacgata aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc caaatggctg
      901 ttttttgctt ttcttttgct ttttcaatag ctgtatctag taaaaatgta ccgatacctt
      961 gatgtttaaa tttttttcgt acatagattc gctcgatttc taaaaaatga tcagccattt
     1021 cctcagattg actcgttcct gtatttagtt tcaggtaacc cgcaggctct tgatcgatac
     1081 tcactaggaa aaaatcggtt tctttatctt ttagctcttc tgtaagtttt tctagtgcat
     1141 aagcttgtgt caagtaatca gctaaatgct ccggtgaatt atcttttcca aatgtatctg
     1201 taaatgtttc gatactaatt tgttgtagta agtctacatc tgaaggcgtc atttttcgaa
     1261 tttcgtattt attcacggct tcttcctttc aatagatcct tttattgcct tttttgacaa
     1321 aattccaatc ttcgctaaca ttcttgcata ctttatttaa aagatattcc aattgttttg
     1381 cttcttcgtc agaaagacca tttaatgcga ctgcattgga gtagttgttt tctcgaatga
     1441 tgaaaggata gatttctgct ccttttttag tcggatagag tttcttgatt tttttatttt
     1501 ctttatcttc caaacgttcg atcatcccat tggattctaa tttcttgatt gctcgggcag
     1561 tcgttgtacg atctactttg atcatttctg ctagtttttc ttggatgatt ccaggatttt
     1621 cgcaaatacg tactaaataa agatactgtc ctcttgttaa atcgacttct ttgaattcga
     1681 tattgctaat agaatctagc gctcgggcaa tcattccgat gtctcgaagg atttcagcca
     1741 tttcatccca cctttatttt gtttgtaaga ctagtttacc tctttttgtt gcaaatgcaa
     1801 ctattttcaa taaaaaaatc aagttctttt ttaataacta gtaaagtata ttcttttctt
     1861 cttttacgaa aaagctcaat cacaatagat ctggtttttg ttttctaaaa taagtaactg
     1921 tttcttcgtt gttcgctttt ctgatagctt tggcaactct tctatactaa aaaatcccat
     1981 gttggaagtt tcattattct caataaactt ggcctcttca ccgtggatcg cacaagcaaa
     2041 aacaagttta taatactgaa aaagctgtgg aatatctttt tgtttattcg tatcaaaaac
     2101 tgctcgcaac tgagtagttt ctactgttaa tcctgtttct tcatagactt ctttacgcac
     2161 attttcttca ggagacaatc ctatttcagc aaatccacca ggaagtgacc attctttcgt
     2221 ccgtttatct tcgattagta ggaccttgcc atcttttttg atataggcac gaacatctac
     2281 ttttggtgtt ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt cgaaagtttc
     2341 ttttcctatt tcactgatca gttccaatga aatcgtccgg atctcctcaa aacgctcttt
     2401 atcaaataca tcttttccat aatatagacc agcttgagaa agtgcgagta gtcttttgta
     2461 ttgagccaac ttcttgttca ttctcttccc ttctttttta gcaaatgtat gactatgtaa
     2521 tcaagtatag aagactggtt taattacggc aaataaatat aatgaaatct gcaataaata
     2581 aaagaatgtt aaaaaactcg agaacaaaag acattcgact tctctggggc tgtgaataaa
     2641 tggcgttcaa aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa tatgacgagc
     2701 tattcttgtc tggctgttct tattaattga ggcaggacag ctttttcaca acctccccac
     2761 taaacggtgt tctcctacca actcctcggt cacaagtcac aatattcatc aaacatgaga
     2821 agctgttttt ttgaaattgc tccttgtgac tcggagctga acgacaggct cacgacctct
     2881 cggtaaacag cgttcaaaag ccaatttttc gatattaagc catttctttg ttacttattg
     2941 tcattgattt ctttttctaa tttatccaat agtggagtgt aatccacttg agtatagtct
     3001 gttacatcaa attcatatag tgttcctaac tggaatttgt catattttcg gtcggcgatg
     3061 atctttttga atgcttcttt cgtaatatga ttggtccctt tcgttcgatc ttccaatacg
     3121 tcaccatagt gataatgatc catgatatag ctcaaatgac gatctgattc acgatcacgt
     3181 ttgtaccgac gttgacataa cacttcaaag tctgctgtta ggcgaatcgt gatcacctga
     3241 tatccgtatc tatctgctaa ttcttggagt cgtgcttttt gcttatcact aaaaggatac
     3301 tcggacacaa tcactttctt tcctgcttcc atatacaaat caagtgcttt gtaatagaaa
     3361 ggataaactt tcgtcctttc ttgttccgcc cgttcttcca aattgttaaa cccgatggat
     3421 tctgcatagt attccttgat ttcatcgagc gtgatggcat acatatctgg aaatcgttcc
     3481 cgtagtttat tcataagata tgtttttcct gtaccaggac taccagccag aagaatgaaa
     3541 tactttttca tctcatttat ttcctaagtt aattggactt tccaagttca taaacagtcc
     3601 aatcttatgc ttgacaattt cttttactgc ttcatttgct ggaatcacat tatggcgaag
     3661 agacttgttg atttctgttt cactaaaggt ttctttagct actttcgtcc agttgactaa
     3721 aagctccgtt ccaacattga atttgcggat cccgttattg atcaattcag gataatcttc
     3781 ttctttcaca cctgttcctc catggataac tagggggaca tcaactactg catcgatttc
     3841 tttcagcaag ggaatgttta cttctgtttt tgatttgaat tgtccatgat tcgtaccgat
     3901 agcaatagct aatgcatcca cgcctgtagc ttctacaaat ttcactgcgt cttccggtct
     3961 tgtatatacc ttgtcatttt cagcgacatg gattccttct tctgtaccgc cgattgttcc
     4021 aagttctcct tctacggaaa caccgtgcgc atgggcatat tctactactg cttttgtctt
     4081 caggatattt tctttgaaag gcagatggga cccatcatac atgacagaag tatatccgct
     4141 gtcgattgct tctttgatat catcaaagtc tcgggcgtgg tctaagtgca agactgcatc
     4201 aaccatttct tcttctgcaa tagctgtcat tacattgact aacacatgca tgccgatata
     4261 tttagcggta tcgacacttg tttgaataat gatcggcgaa cccatttctt ttgctgcgcc
     4321 tagcatagca ggaagcattt ctaggttatg cgtattaaaa gctccaactg taaaattcaa
     4381 ctcttctgct gtttttgtta cttctttcaa tgttgtatac atcttttatt gccaccttat
     4441 ctttttattt gtttcctttg atcgttactt gttttgcaat ttgtcgcatc tcatccattt
     4501 tgcccatgta gtaatcgatc ccttgatcat caccagcacg agcagcatct gcacgcttct
     4561 ggttatagag gctcatcagt tttttatacc catctccgat cgtctgttta ttttccaaac
     4621 tcttttctaa atattggatg gcttgatcgt tcacatacaa ttcagaaaat ttaagtccaa
     4681 cttgttcata aagtttcgct aatttatgct tatcttgggt agtggaaatc tcttgtttca
     4741 attgtgtaat ctctgattga atcttttcgg tcgcttctgg cgatagggct ttttcttctt
     4801 ttgccagaac ttttttcggt tctttttctt ttttcttaaa aaatccaaac atcattttcc
     4861 ctactttcaa ttaaaatgct ttcaaaactg ggcttaagat ccaggcatat aagagtgctg
     4921 cggctactgt atctacatcg gttgctgtag cattgatgaa tcccatcgaa ttaaagatcg
     4981 ttactagcaa agcgggcaat aatgtaataa agaaaccatg agcaataccg ccaatgatcg
     5041 ctcctcggcg tccgccaact gcgttgccaa atatccctgc tgtccctccg gcaaagaaat
     5101 tggttagcat accaggtaaa atcatcgcta agccgaacat gggcaaagta aacatggcaa
     5161 taatcgtacc aatcgttgtc gtaatgaaac ctaaaatgac agcatttggg ctataaggaa
     5221 agaaaactgg acaatctaag gcagggatcg catcaggaac taatttcata gctatccctc
     5281 gaaatgccgg cacgatttca cctaacagta gacgtacacc agctagcaat acatacacac
     5341 ccactacgaa ttggatggct tgtaaaaagg caaacatgat atagttttga tctcctgata
     5401 attcagaagc gaatggttcg cctgcaaata aaacagtgat gatatatagc gggaccatca
     5461 cgaccatgac agacaaataa gtatcttgca gaaattcgaa atgtgtcggc aacttgatat
     5521 cttcgatcga ctttttattt tcgcctttct caccaaaaag cttagcaaca ccggcttcaa
     5581 ataagtaccc gatcgtacaa aaatgaccta aagcaatatc atttgatcca gtgattttac
     5641 gaatgatcgg ttgcgctact gccggcatag caatggcgaa acaagctccg ataaaaccac
     5701 cgaccaagat caatacgatc cctcgcagac cggcaaaata gccaaagact gtcgtcatcg
     5761 ttgccatcca taaaattgct tgaccagtta aaaaaatgta cttccatttt gtgaaacgag
     5821 ccaaaataat attgaataca aaaatggcta agaacgtcag tgcaatatct ctacctaaac
     5881 ccaaatcatt cattgcttga ccattgatag attcaataga agggatgatc ccttgcatat
     5941 gaaatccttc agtaaagatt ttcccgaaat aagttaaact tccaacgatg atgctcgaac
     6001 cggcactcaa cacttgaaat cctaggagtg tctttaacgt gccagagatg acttgtcctg
     6061 ctgatttctt ctgcagtagt agacccaaca tagcaatcaa tgcaatggtg atcgatgcct
     6121 gagtcaaaat attttcgatg ataaaattga taacgcccat gtttttaccc tcttttcttt
     6181 ttgtatttag ataactccct tatcttgcag aactggggtg attttttctt caatttcatt
     6241 ttttgaaaca atatttttca aatagatcat cgccgtttta ggatcaactg cgaacttttc
     6301 gaactgcgaa cgaaaatttt ctgctgtgat gatcacatct ggtttcatgg aaactgcgga
     6361 agaaatatca caatggtcca gtttggcttc tactcctaat ttgttcaaaa catcctcagc
     6421 agacatctgt gctgcaaaac tacttcctaa acctgcaccg catacgaata aaatgttcaa
     6481 tttgttcatc tttgttctcc cccgtatcat ttattatttc tcaactactg aaaaaagcag
     6541 ttgtttgaaa tcttcgatat ttgtgcattc aatcaatcga tccaacttct cttcgttgtt
     6601 gatcaattca attagttctt tcatgatgtt cagatgcgag taggagtcta ctgctgccaa
     6661 acaaaaaata atctttactg gatccatttc aggattaccg aaatcgattg gttgctcaat
     6721 cgtagctacg ctgactccta atttattgac cccatcttct ggtcgggcat gggctagtgc
     6781 catgtgtttg ccaatcacga tatacggtcc gtaatcattc actgcacgaa tcatcgcttc
     6841 gacatagcgc tcttcgacaa tgttttcttt aaccaatggt ctagcagcta gttcaatcga
     6901 ttcctcccaa gtagtggctt tttctttgat catgatattg ctgtccgtca aaatatcttt
     6961 taacataggc tggatctccc ttttgttgat ttctaaattg tttgtttcga ataatttttc
     7021 taacttgcca tagatgattc gatctactcg gccaccactt tctttcatga catctattat
     7081 ttgagtgaat aactccgtcg attcatgact gttaggctct aaccgctgta gttcttcatt
     7141 tcttttcaaa aagtcttcta taaccaagat attcgtctca gttaaaatcg gatcgacaac
     7201 taaaagcggc ttatttcgat aattcaattg aaaggtgctg aaaaccagat tgacatccaa
     7261 tttatctact aattcgattt cttttgaact taaaactgca gtaatctcga tctgaggaaa
     7321 cttttctttc agattctctg ctaacagatt tccagtagcc atcccatggt tacagacaac
     7381 gactgattta taaacataat tcaagtctcg cttgattaca ctggcgatcg tcgaaaagtg
     7441 gatgacaaga aaagctatct cgtcctccag tatcttgcca ccaattactt cttcgatcgt
     7501 aggaacaaaa tgagtgactg cgcggtagat tgggaaataa tttgttcgga tgttttcttt
     7561 caatggattc atgacttgga tatgattctt gattcttaca atcagtgcag ccatgtgctt
     7621 atagagattt tcgaataaag cttcttcctt tagatggaaa ggtacgtgtg tctcgttctc
     7681 tacaaattgg atcaattgga tcgttagtaa ttgagcttgt acccactcga ttgaattgga
     7741 gatatctcta gcattgaacg tctcaatagt gaaacgaaga taatttcttt ctacttgcgg
     7801 gacattgtcc aattgaaatt cctcgatcat gtggtcaata aatgcagaaa agctactgtc
     7861 ttttatttct gtatttttcc aattaatggc agagataaat tcatgtttcc tgattcgctg
     7921 gatccagatc aatgtaaaaa gcaggatttg ttttttataa atatcatctt cttgtttcaa
     7981 caacttattg gaatagatct cctgtatttt tttgatttcc accaaaggaa aataatggag
     8041 aaagattttt tgagccggtg ttaattgtgc atcttgattc ataaagaagt cggtcattcc
     8101 cagattctga ttgataaaat catagatcat cgtacggata gatcgttctg aacctttata
     8161 aaccagtcct tgctttccat aactgacgat ctctatccca taactgacca gtgttgctcg
     8221 taaccgtctc atatcttcat ctaatgtact tttagagatt tgaaattctt cttctttttt
     8281 gtttagatgg atcgggattc ggctgtaagc aacggctaaa actagatcaa aaatacgttc
     8341 gcgccgattc aaaaagccgt ctttcacatc ttttatttca gtttctaatc gagcttgctt
     8401 ttccttttcc agcttcaaca aaaaaacttt gttgcgaatt gtacggatct ccggaagctt
     8461 tttactgctc aaccagatat tcgcttcaag aacttcctga cggatcgtcc gctgactgac
     8521 attgaactca cttgctagat gttttgcttt tactggatat tgggctgtca tgattctgtg
     8581 aagtatttca attgttcttg ttttcataga aatgatctcc ttctgatttc aagtatatgg
     8641 ttccgcttac ataataaaaa gtagatattt ctgccacttg aagtggaaag aaagtaagca
     8701 aagcaaacaa aaaataagcc atatgctttt tcatctcctc aacgaaaaag acatacgcct
     8761 attattacaa aaattttgcc catctataca aaaaagaggt tgtgaaaaag ctgtcctgca
     8821 tcaagtaata agaacaacta aactaaaata gtgcttccta tttttcgttt ggttgttctt
     8881 attatcgcag atgcaagctt ttaaacacca tttagtggag aggttgtgac agagatgttc
     8941 agccttgagc gataagaagc gattttgaaa aatcgcttcc catattttac aaaatcgtgc
     9001 cttattgccg ataggctatc tctggcacac tgtttattca gaaaaaggag ctgcgacaag
     9061 gcttttgtca caactccgtt cataaatatt tcctgtcaat ccagcataat cggctgaata
     9121 aaatcggctt gccatctttt ttctttctca gaaaaatcaa aataccatat ctgagaaaaa
     9181 tactgtcgtt gtgtcttact tcctgagatc aatcgcccat cttttcgatc gactgaataa
     9241 tcacagcatc tgaaatcaat tcggatggca aaactatttt cgtggatctg tcgataattt
     9301 cctacttcct gaatagttat ttttttagta tgatttctta caccgtctgc tttgtttttt
     9361 tgcagaactt tctgatgttc ggcaaacagc ttgtctgtat aacagcttct ggctttttca
     9421 ggagtttctt cttcccaagc ggattggatc ttcatgaata tcttttggat ttccagaaac
     9481 tgtgctttct tttctttagc cgttcctgac atgcgggaca atatctccgc tttcagcctt
     9541 ttcttttgtc ttttattctt gacgatagaa aaagcagaaa cccctcctac tgacaaaaca
     9601 gctaatgcaa gaaatcccgt ttgctcataa ctagaaggac ctgtacctgg accataatac
     9661 ccataagaat ctctgccgta agaacgactg ccaaaataac tgcccccata tgaacgacca
     9721 ccaaatgaac ctctgcccga acttcgaccg ctagatgtac ctccaccatg accacccgcg
     9781 acagcgaatc cttgggtcgg cagagcaaac aaacataggc ccactaacaa aatgattgct
     9841 aaataattga tttttttcat tgtcttcctc gttatctttt attacaaaaa actattttta
     9901 tgatagcctt gctaaaagga agagtcaata accagatgca taaactgttt ttttagcaat
     9961 tttgtcttat ttagtcattg agtaacatcg gacaaatgta tgtgtcggca gtccataatt
    10021 cctctatctt cagcatgaac aagcttgcag acaagagatc tgcgcttcct ccaggagaga
    10081 tttggttttc ctgacaaaag tgcaacagtg ctgtaaatgg acgatttggt aaagcacttt
    10141 gactagcagc atgagcaagt ttttgcattt ccactaacgt tttctgatca ctgcgatgca
    10201 atacgcatgt gtcatctagc gaactcatca aaactaacaa catatgcatt tgtttctgta
    10261 catatgaatc attatctagc tccttagctg cttggattgc aagcctgata tgcggatacc
    10321 ctaatatcgc ttcttcatat gcaccattta cgccatattt ttgcttcacc ctatgaccat
    10381 gagtggtttt tttatgacga tattttgtat caggaaaagc agcaagcttg ctgacatcat
    10441 caagtatttt cgataaagaa aaaggctgtt ccatactgat tttttgtgca atgacactga
    10501 tcaatagccc cattgcccaa atcgctcctt tatgggtatt gatgccattt gtagcaatca
    10561 gcattgcctt ttccgctttt cgcccaatct cagcaattct ttctcttaac tcctgatcaa
    10621 tagcatgatg ccatgctatt tgagccattt cttcaaaaaa aggagttagc gtcttagcag
    10681 acttaagaaa taaagcatag tccatatcgg aatgtgcacc cgcatccagt gcatccacta
    10741 atccaggttt tggacttaat gttacttctg cgatcaatgc ttcctctacc aacgcagcga
    10801 gttgctgagt cactgctttt tttcgttcgt catttatcat gacactcact ccttcttcct
    10861 actattactt gctgaaacct tttttgatag ctacccaaat gatatgaagt aaccagctta
    10921 aagcaaatgc acaacttgcc gtcattagat aagctacaag aatcatgact cctgaaggta
    10981 actgcagcca actatcttta aacaataata acagtacttg gaaaacaaat acattggcta
    11041 aatacgtacg ataagcataa acagacaacc atttgaacaa tggcaatttt ggcgaatcgt
    11101 ttacaatcat tttggaagcg cctatgaaga cagcaaagat gatgactaaa ctgtataaac
    11161 tttggatcgt attcaagtaa ggtgcattcg caaagctcaa gtctcctgga tgtgcgagca
    11221 atgaacggac agaaaagaac gcgataacta aggcaacagg taagaaaaga taacgtactc
    11281 tgtccaaata ttgatagact ttttcgtgat aaacaaatgc ggctacccca taaatcatga
    11341 aactagcaac aaaccgatct aataagtacc agctgtcatg ataaggccca gtcaatactt
    11401 gtgtttgata gaacagatac catgcgacat ataaaatcgt tgaaccaatc aatactggcc
    11461 atacatattt tttattagca aatactttgt aaccgagcca aacaaaaaac ggcatgaaca
    11521 attggatctg cagcatcata acggtatacc ataaatgagg cgctccatca cctgtgacat
    11581 atttcagtaa aaagcttgca gtatccgtat acggagtttg ttgttggact gctggaaata
    11641 gccaaagata agccgtcgtc catagaagat acggaacgac tagttcaaaa aacttttctt
    11701 tcaaaaattc ccaataagac aaatgctgac ttgtttttac catattataa acaattgcaa
    11761 aaataaacat tggtgctgtg tatttaacga tgacgtaaaa ggtaccaaga aaagctgtat
    11821 ttgttgcgct gtggctgttt gccaaagcaa agctcaatat cgtttgcatc ataacagccg
    11881 ttacagctga tactttctgg aagtcactta cgttgatttt gatttttttg ttttcaaaca
    11941 tctctttctc tctctttttc tttattacca aagttctttg gttaatttag gtccttcgac
    12001 tgttttcaat agaatttttt tctctggctc tctcaaatac tcttttagtg aaaaaccgcc
    12061 ttgccgagtc actacttggg tatcgaattg ctcaaaaaaa gcaggatgcg attgaattac
    12121 ctgaattggc aattcaatcg gtgaatttgc gtacagcagc aaatcaaaat cactggtttc
    12181 ttttactgcc ggaatcccag tcgataattc aaatcctaag ctgccgccga ttccccattt
    12241 acatcctgat aatatgtttc tagcagcatg aaattgctga taaacaggat aatccatgat
    12301 tttctcttgt ctatacgatt cttgttcgat gatgtcccac ggatgaatga cttgtttgac
    12361 ggcatgtttt gagatttctg ttgaccatct ctgattgcgg gcaggtccac gtaaaccaat
    12421 cggtagtatt tctgcgtgat ccgtcgacgc acgacgaact gttccgaaca tcttttcagt
    12481 tagaacgacc cattcaggca aggtgaaccc ttctatttca tgtttatcaa aaaggacaat
    12541 atcatgtgct tgaatcattt tttatgcatc ccattcttcg tccattttgg cacggacttt
    12601 atttgtttcg acacgtccga atgttttggc tgtttcgttt gtgtatcgat ttgtcagcat
    12661 cgtgtcattt gtattgcgga catcttgaat ggcttcattg attgcttcga tgactttgct
    12721 gacattttgt tctgaagcag cttggtcggt gatatcttct aggaaatgat agatcgctcc
    12781 tagttttgta taattattga tatcgtaagc gatagctgga actttttcag cagctgcttc
    12841 gatttcagca accgaacgtt ttgtcacacg tgcagcactt tctttagaca tcgcttggac
    12901 ggtaatttct ttattgttta aagcaatcaa gcggcttgat tgtaatccat gtgctaaaaa
    12961 tgcacctgaa atagcatttc ctgcaatcaa tccaataact ggatgtccag cttgacggag
    13021 ttttgcataa gcagcagcac tattagccaa agccacatgg ataccgatca attcttcgtt
    13081 atagccgtat gcttggcttg gtacatcgat cacaacgatg attggccgtt tcactttttt
    13141 gtctttatct tcttcgtaaa cgtgatccaa aatttgtccc atgcggaaac cttcaactaa
    13201 accaacttca ccgtttcgta cacggtacat cgggttttta ggatcaggta cgattgatgt
    13261 cacgatggct tcttctatga atttacttga tttaccataa tacacagtac taatagaaga
    13321 agttggattt ttcatgccag ttagtgccgc aaaccaatcg taaccaacac ttgctgcagc
    13381 tggttcattt ccttctgcta cttctggtaa gtcaattgat tttgcttgat ggttagcaat
    13441 tgcttcattg taatcgtcaa tggccattgg ttttgaaaga tctaaagaat caagtaatga
    13501 taaataaaat gctgagcttt cactgcgatg cgtgtctttt cgttcgtcca ttgctgcgat
    13561 gacagcagct ttgattgctt ctacactatc agtcactaat tcatcaatga tttttgattc
    13621 tacccgttga cgagatccta atgtattcca aatcaagtct ttgtcactag aatcaaattc
    13681 tcgaacgcct gcttcttgtt caataacttc tggtccattc aaaccgactc tcgctttttt
    13741 tgttgcgatt aaataactca ttaatgctga agtaatcgac ataccgccaa atgatcctac
    13801 acgtcctgga atcaaaccga tgactggtac atattgtttt agagcaatga ttacatttcc
    13861 gatttctgaa atagataaca gtccgtagtt tgcttcttgc aaacgaacac cacctgtatc
    13921 tagtacaatg actggaaaga tctcatttcc ttttttattt tcttctaaca catgttcaag
    13981 tgcagcaacg atttttgcac cggaaacttc accgataccg ccaccttgga attttccttc
    14041 catagcgatt actactgctt gtttgccatt gatcgtgcca cggctgacaa cgatcccgtc
    14101 atcactttca gggacgattc cttgtgctgg taaacttggc gcgatgattt gatcgaatgg
    14161 atcaatcaat tcacgtccat tgtcaagtag agcgttcact cgttctctgg cgtttaattc
    14221 tacaaaacta tttttcatct gaaagttcct ccattgcttg cgtcaatcgt aggtacacaa
    14281 ctcctggtgt agcaccgaag tcattgatca cgatatctgc tgaaattgga taacgatcaa
    14341 aaaatctcgt taatacattg ttccatactt cgccgaagcc atcgcttcct gtacaaattt
    14401 ttacagtaga ttctgttttt tctgttggaa acatcaagat ttccaaatct cctgaaccga
    14461 ccactcctac atggattttc ttattgatcg gctgtttcgt tggatattta taagctaatt
    14521 gttccatgga tctctcccct ttttgtaaaa cagagcatac acgttctgct tattcgtctt
    14581 tcttcttcca tctgggtaaa tggcgacaaa aggtttttct cgccaatttt atgaccaagt
    14641 tctgaatttc attggtggtt catacaggcc atctgaccat tcgatcaaat catcgatggt
    14701 ttgtgcagct aataatgaac gtttcgcttc actacgtttg atgcctagat cttctggtaa
    14761 tttcacgatg ccggctgcac gcaagctatc tagtgattct ttcgtgctag tcattccaag
    14821 aggtgtaaca ccgccaattg ctgcaattgc tgcttgtcgt tcttcttttg tttttgcttt
    14881 gtacaaataa gcaattcctt cttctgtcac gatatgtgta gtatcttcgc tataaatcat
    14941 cactggtgca ttggctaagc ctgcttgttt ttgaacagct agtgcatcta attcttcgac
    15001 aaagactggt tttttatttg aaccatatgt ttcaaccatt tgaacaacta atttttggcc
    15061 tttccctaat ggatcatcat tgtcgcgaag tgattgccat gctggtgtag aatgacgacg
    15121 accgccagga ttatgtccca tgttaggcgc accgccaaaa ccagataaac gtccttttgt
    15181 tacggtagaa gagtttcctt cgtaatctaa ttgcaaggtt gaaccgataa acatatcgat
    15241 ggcatattgt cctgcagctt gtgataatgt tcggttactg cgcatcgtgc cgtctttacc
    15301 gacaaaaaag acatctgggc gagcagcaat atatttttcc ataccaactt cgccaccaaa
    15361 actatggatg ctttcaaccc aacctgattc gatggctgga atcatcgttg gatgtgggtt
    15421 caatacccag ttcttcgcga ttttcccttt taaccctagt gattcaccat aagttggcag
    15481 taacaattcg atagctgctg tattgaatcc aataccgtga ttcaaagatt gtacgccgtg
    15541 ttctgcgtag attcctttga ttgccatcat tgccattaga atttgcaatt ctgtgatgtt
    15601 ttgaggatct cgtgtgaata aagcttctaa ttgataaggt ttatctgctt tcacgacaac
    15661 gtctacccag tcaccaggga tatctacacg tggtaaagaa tcgacaactt cgttgacttg
    15721 aacgatcacg ataccatctt tgaaagctgc agcttcgatg attgttggtg tttcttccgt
    15781 attgaaacct gtgtacaagt tgccgtcttt atctgcttta tctgcagcta ctaaaacaac
    15841 atttggaatc aaatcgatga ataagcgtcc ataaagttcc aaataagtat ggatatcacc
    15901 taatttcatt gtgccgtctt caatcatttg ggcgacacgc aagctttgtg caccagcata
    15961 agagaaatcg atcttgcttg cgattccttt ttcaaaaata ttcaaatgtt ctggtcgtga
    16021 gatactagac atgatcaaat gcagatcatt cactttttct gcatctactt tttcaagact
    16081 ttctgaaaga aaacttgctt gtttttgatt gtcgccttct aatacaactt tgtcgccggg
    16141 agcaatcagt ttttccatta cttctacgat tttttcagtt tctacatact tgccattgag
    16201 caagttgctg attttttcca atcgctgcgc ttttttttcg cgtttagaag cccagttgcg
    16261 gtttggtttt tccataatta gtcatctcca tttttgatat agaaacgtgg cgtcatcaat
    16321 accatgttgg tctagattga tcgtacggat ccctttatct acaccaaatt ttgtatgaat
    16381 cagctttgtc aaagtattgc cggggggaaa ttcaatcgtc agatccattc ctaattcttt
    16441 tgccaagtcc atcatttggt tccactgcac agggaaagct aggttttcca acaaatcttt
    16501 acgaatctct tctgcgtcct gagtggctct tccatccgta ttttttatat acaaacatct
    16561 agcatcagat agatggacag actgcatata cggcttgaat ttcttgaccg tatcattcat
    16621 caagagacag tgagatggca cagggacttt cagcagcttc gctgtttgcg cttggtgccg
    16681 tttggcttct gccaacgtaa cggataaacc ttcgatactc ccagataacg tatgttgcat
    16741 cggacaattt tcatttgaca gataaactgg atggtcagga tcaaatgttt ttgctacaat
    16801 ttcctcagct tcgcttctcg tcacacctac tactacgccc attgcataac cgcttggata
    16861 agcttctttc attaagactg cgcggtgata gactagttcc gctgcattct caaatctcaa
    16921 agatcgacaa gcaacagctg cacaaaaagc accaatcgaa tgtcctgctg caatatcagg
    16981 ctgaataccg tttttttcca tttcatctaa ataaaatact gcttttgtca gtaatgctag
    17041 ctggataaaa accgtatctt gatagttttc ttctttatca actaaccgat accccgtcac
    17101 attttctaaa cgttccatgt acatttcagg gatatccgct aacatcccta acttctgact
    17161 gccttgtcca ggaaataaga aacctgtact catgagcctc gcctcctcag cacttacttt
    17221 ttccactgct atcttagtca atttctgcta taataaaaaa tgccaaaaaa agaaaaaacc
    17281 ataacaaaaa ggtatggatg ggatggataa ttatgaaatt gcttcatctg cagtatttta
    17341 aaaaagtatt cgaaacaaaa aatgttaccc aagcagccaa agaattattt atttctcaac
    17401 ctgccttgag ccgtgcgatc aaacatttgg aaaacgaatt aggtgtccct ttgttttacc
    17461 atagcggcag aaacattgag acgaccgttt atgcagaaga attttatcca tatgcagtca
    17521 agacattaga cacattagaa gaaggaatca aattattgtc ttcggtcaat gagaagatcg
    17581 ttacttccgt catattgtat ttagaggtag cctctgtttc tattccgaac ttggtccgaa
    17641 tttttatgga aaagcatcct gatatccaat taacgatcat gcaacatcat ttaccagatg
    17701 atattaccga tcctgtactg tacatcactt cggagatgaa gcctggcatg accaatattc
    17761 ctatcatgaa agaacctgtt tatgtcgcca ttcctaagaa acatcctctt gctaaaaaag
    17821 aacatctgca attagaagat atccaacgca cccccatctt gatgctgtca aagagcaacg
    17881 ccttccgtca tacactggat gcagcacttg aagcgaaaag tatcgaattg acgatcagtt
    17941 cgacgacaga tgatcctgcg acattacgtt ctattgtcaa gcaaggatta gctgttagct
    18001 ttttcccaaa agtttcttgg tcttacgata aaaatgatcc tttcgtcttg cgggaaatca
    18061 aagatttgcc cttgacacga acaatttatt tatcttctag cttcacagaa gatcatcctt
    18121 tgatcaaaat gattgcaaaa acgatgcggg aattttattt aggaaaataa aaaaaaaaga
    18181 gctgggacaa aattcttgtc gcagctcttt tttcgaacaa acggtgttcc agaagtaatt
    18241 tcttagaaaa taagccgaaa tttcccaaaa atttgagaag caattttcgt aaattccttc
    18301 ttatttcttg aagctaaaca tttctgtcac aacctcttct ctattgtcct agacattcat
    18361 tgcgttgatt taaaacttcc atcaatcaaa tctgctagtt catctagctt ttctatttta
    18421 cgtagatcaa tagagatcca atgttcttta ttcatgtgat aggctggaaa atacccctct
    18481 tttcctcgca aaattccgcc aagttcagga tcgaccttca aatcaagaat attgatgatc
    18541 ctatggtcac ttgcatcatc atagattttt tcttctggaa cagacatcag gaccccaaac
    18601 cattttttgc ttttttgatg tctgaatacg caataatcag gaaatttttt aaaaatatat
    18661 tctggagaca aattgtatgt ttcattgatg tgatcgatta gttctttttt cgttagcatc
    18721 tgcttccatc tctttcttat tgattcgctt tattcaaaga taccacgtaa gaatagaaaa
    18781 atgaatacaa gtagttgcta atcaaatact tcgatatttt tcttttttat caactcttaa
    18841 atgttcaatc cgcctgtgtt ggtcttgcta ccatctacta ttaaaatgtt tattattgtt
    18901 tgcccaccta ttttttgatt ttagtggtat catttttcaa gttcatgatg aaattcttat
    18961 catcagtaaa tgctccagaa aaataaacaa tgattgaaaa atcagttgaa acttgaccac
    19021 cgtttatttt ctccgtattc ttagaataga agggagtctg attgatgtat tcactaatga
    19081 aaaaaatcat taccgaaaaa gatattcgac gtcatgtttc tttagtagaa caattgctga
    19141 atcatacaaa gataaccgta aatgaattag ctgaaatcat agggacaacc gaacgaacga
    19201 ttttttctga ccttcaatct attcgctctc agctgcccga aggctgggac atcatcagcg
    19261 atcaagcagg aatcagtttg caaaaccagc aaaatttatt aacaaatgac ctttgggaga
    19321 tttttttcaa acaatcagtc agtgtcgaac tgctgaaaaa tctgcttttt acaaaaaaag
    19381 ttgctgtccc tgattttcta gccgaccatg ggctttctta tgggacattg aaaagacatg
    19441 tgactaaaat caatcagcgt ttagcaagtt acgacttgca gatcgatttg acgaaatata
    19501 cagcttgtat tcttggaaaa gagcgtgcga ttcgtacttt ttatcatcga ttgctgattc
    19561 ctttcactca taacaactat tttttcgagg attattcgat ccatgaatcc cattattttc
    19621 aatttttaag aaacctcagt caaactgaac tagcggttga aacagaagag attttcggaa
    19681 catgttggtt ctttatcaat acgatccgta taaaagcgaa ttgccgatta gattcttcta
    19741 tccataccaa cagcacactc tcctccctgt atgactctgc attgaaaaaa ttgtatttaa
    19801 cggaaggtat ttatcttaaa gacactgagc tttcttttgc ttctttctgc tttctggaaa
    19861 gttggaatta caacaataac tatggacaag aaaccgctcg ctgcctgcat cattctccat
    19921 ttttagaggt cctcgaaacg tttgtagagg aactagcttc agagctgtct cttgatcaac
    19981 taaaaaaaac atctctgact gataacttgg ctctcctcat attaaaatat catgaatcgc
    20041 cgatattgat tgaagcattg gaccgtcaat accatcgttt tttagaaaca tatgatcagc
    20101 aatatgcaca cttgtatcaa cagaaagaga atttactgaa cgatctaaaa aaagaaatag
    20161 aaatcaatga accaaattac tttctccaac tcttgtctct actgatccaa caaacgattt
    20221 tttctatcaa accgaatttg ttcaatgtct attttatttt ccaaggagaa ccttcatgga
    20281 aggcgttctt gcagcaggaa ttaaaagatt acttaggaaa acgtgtctgc ttcttaccta
    20341 tcgaactaac cgatctttcc actatttctt ttgaaaaaac agatatcttg atttccaatt
    20401 tccctctaga ttctctagat atccctattg tatacatttc ttctattcca acgaaaaatg
    20461 agctcaatcg tttgactgaa ctcactttca aaactttttt atagtaacgg ctagcgactg
    20521 tattctctag ctgttttttc ttttttgcga aacagtttgt tcatgatccc tttttctatt
    20581 tctacaacta agaatagaag aatcacattt gctccaatca ataactgttg tgtcatcgtt
    20641 aaacttactg taccgatcag ctgctggccg actggtagaa aaataaccat cccttgcaat
    20701 aaagcaagga tacctaatga aagaaaaagc gctttgtttt gcaaaagacc ttggttcaat
    20761 gacggcttgg acaactctct gcaattgatc atgtaaacag cttgagctaa gacgatattt
    20821 tgcagaagaa tggtttgctg taagctttgc ccttcaaact gcatagccaa taaataggca
    20881 ggaaccatga tcagtaggga gacatagatg atccgaaaca aatgataacc agataggatt
    20941 ccttgctttg gatctcttgg tgggcgcttc atgatttctg gatctggcga ctcgaaccct
    21001 agtgcataag ataaagtgat tgtcgttacc atattgaccc ataaaatctg tactggggac
    21061 aaaggtagcg ggcgattcag cataagtgcc caaatcaaga tcaatccttg agctaatgca
    21121 gttggcaaga agaaattgat ggttttttgc aaattatcca aaatccgccg gccctctttt
    21181 acggcttttg caatcgtatg gaaattatca tctcctaaaa ccatatcagc tgcctgtttg
    21241 cttacttcac tacctttgat tcccatagca attcctacat cggatctttt caaagcaggt
    21301 gcatcattta cgccgtctcc ggtcattccg acgatttctc catttttctg caaagcttta
    21361 acaattcgca gtttatgttc tggtgtcgtt cgagcaaaga catcgactat tggcacttgc
    21421 tgaatcaact cttcatcaga cataagatcc aaatcgatcc cttctaaaac tttggcagta
    21481 tgttttaagc caatttgttc cgcaatcgct tttgctgtgt ctttgtgatc ccctgtaatc
    21541 attttcactg agatccctgc ttgttggcat tcctttacag ctttgactgc actttctttt
    21601 ggcggatcga tcaatccggc gatccctgca aaagttaatt gggttaaagt ctcatgcgtg
    21661 agttcctgtt tcgaatctac ttttttataa gcaaatccta aaacacgctg accttttctc
    21721 gcaaattctg cagctgtctg actccatgct tcttgttctg cagaagaaag agttgattta
    21781 gcaaataata cttctggtgc acctttgacg taaatcaccg ctcctttttc ctccgcatgc
    21841 atcgttgcca tatatttgta ttcagaactg aacggaatct ttgcttctat ttttttcaaa
    21901 ggtagattgg ctgtttctgt gtgtcgaatc agcgcaactt ctgtcggatt gccacttaaa
    21961 ttttctgttt tctgttcttc ttttgttttt aattcctgac agtttttcat aatggacaac
    22021 acgcaggctt cttcttttgc agctacatcc acaactgtca tttcattttt tgtcaatgtt
    22081 cctgtcttat ctgaacagat cactgtcata gaacctaatg tttctaccga aggaagtcct
    22141 ttgatgatcg ccttttcttt tgccatttca tgtactccaa cggatagaat catggtcaaa
    22201 acagctggca atccttcggg taccattgac accaccaaag caatcgtagc cgaaagaagg
    22261 atatgccaat ccataccata tcgaaatgtc gtaaaaataa ctaagaatag gctcaaacaa
    22321 agaatgcctt gaaaaatttg tttgtttaat cggttgattt ttttaatcag aggcgtcgtt
    22381 tgttgttcga ccgattgcaa tgcttgattg attttaccga tttctgtcgt gtttcctgtt
    22441 tctacaacga caccgactgc agaaccagat tgtacaagcg ttcctgaaaa agccatattt
    22501 ttctggtcgc cgatcgattc ttcttctgtc aaaacggcat gacttttttc agatgcttct
    22561 gattctcccg tcaaaatcga ttcttccact aataaatcat gtgcttcgat taatcggata
    22621 tctgctggaa cgacatctcc ggcacgtaag acaatgacat ctccttttac cagttctttc
    22681 gcagggattt tttcttgcat cccgtttgat agtactatcg cttcttgtcc catcagctgc
    22741 ttcaaaccat tcaatgattc ttccgctttt ctttcttgcc agtaactaac gagtccattg
    22801 acgatcacta cgaataaaat aatacttcct tcaatatatt cactagaagc aaatttcaaa
    22861 aaagaggcaa tgatcaacac gatcatcagc agatcagtaa aatgctttgc tatttttttc
    22921 catcgttttg tctgctgcct tgcttttatt tcattatatc cttgatcagt caggcgtttc
    22981 tgtcgttctt cttgcgacaa gccttctgtt gatgtttgat agtttttcaa tacatcttct
    23041 accgtcaatc tgtaccagtt catcctatcc cctcctgttt aattgatact gttattatac
    23101 gcagggaagt tttttcaggc gtatcgatta tttcaggggg gtctgaaata attcgttttg
    23161 caaaatgtat ctgggcctaa ctgatatttc tattgcttat tcttttatat acaaaattta
    23221 agaatacaaa ccagataacc aataaaaaag gaataaagcg tgtcgtgctt tcgaataaca
    23281 agctgaaagc aattgaagta aaaaacacca acaaagcaag atctttatat ctttgtgtag
    23341 tgttccgttt cttttcttta ataaattgaa catgtgcaca caagatcgaa gaccacacaa
    23401 atagaaaaca actcgttgtc atgcctgcca aaagctgaaa aaacaatcga ctatcagcta
    23461 caaaaaaatg tagacaaaga ccggcaaaaa ataacattcc tgtaattaaa ataccgtgta
    23521 aaggtacgcc tttcttagaa agcgcacaaa accaatgggg caaacttttt tcttttgctg
    23581 tttgatataa aatcctgcta gtactgtaga ctgcactatt tgctgaggac aaagaagcaa
    23641 ccaaaatcat tagattgaca attgctgctc cgccttggca atggatcact tgcagcatag
    23701 tcactaaagg actttctttt aaatctaaac aatgccatgg aacaacaaat aaaatcgaca
    23761 aaatcgttcc tatatagaaa aagagaatac gccaaggcag acagttgatt gcttttttca
    23821 aattcttttg cggatcactt gtctcgccaa cggttaagcc gatcatttca attccagcaa
    23881 aactgaataa aaccatagga aatgagacta ccattccagc aaagccaaac ggtagtaatt
    23941 catcgatctg tccaaaattt tctttgattc cgatagactg ggtatgaaaa tctgagacac
    24001 tcaatcctat acccaataag ataaaaagga aaatccccaa gatcttgatg attgagaaaa
    24061 aagattccaa ttcaccaaac cacttgactg caacaaggtt gactgccatt aagatcatta
    24121 gtgcgctacc tccgcttatc aaagctggta catgtacaaa ccagaaatgg atatacaatc
    24181 ctattgctgt taattccgca agtccagctc caagccagca aagccaataa gtccaattga
    24241 caaaaaatgc ccactttttg cctaaataat agtcaacgaa atcactgata gagttaaatg
    24301 tactatttgc taaaaataaa tcacccatgg cccgcatcaa accataaata gccaaaccgc
    24361 atatcaaata aacgagaata acagctggcc ctgctgatgc aattgccttt gctgaaccta
    24421 aaaataatcc tgttccaatt gcgccaccta atgcgattaa ctggatattt cgattagtta
    24481 gagtccgttt cattgccggt ttttccatca agttcacctt tttctttttc aagcagcatg
    24541 gcgacttcct gttcccaaga aggtttcttt attttttcaa tacagagtgg tactacaaac
    24601 aaaatgacac tgatggctgt gactgcaatt agattgatcc atgttccagc tttcatcgtc
    24661 agtccgacag aaaatccgat tgccgcaaat aaaacaataa cgacagtcca aactccaaga
    24721 ttgctctttt tctctttttt tccaattcta aatggtcgat tcaactttgc atgtgtataa
    24781 cgcaatctca aaactgcgat ccccatgatg acatagacga tacagtagat aacagtagtt
    24841 gaatttacga gcatcaaaaa tgcttgattg acgccaggaa tcagcaaata aaaaagtgca
    24901 aagattgaga taatgacaga ctgagtcaaa ataacggatt tggatacgtc aaattgattc
    24961 gtcttccaaa aattgaattt tggtggatac gcaccttttc tagcactttc tgtcactgtc
    25021 tttgctggtc ctgaggccca agcagataat tggactgcta caccaatgaa gacaagtaaa
    25081 ctaaacagat tcacaataag atgtggcaaa ccaagaattt ggcaataaat cgaaatagat
    25141 tgcgcgatat tattcaattc catttgtcct tttggtacga catttgctac aactaaagca
    25201 ttcaaaatat tgacgaaaaa tataaaaatt aacgcggcaa atactccgcg aatatacgtt
    25261 ttaactggat tttccaatcg agtgatatac acagaggaca tttcgatacc agtaaaaata
    25321 aacatgatcg gcgcaaaata aacaaatgag gatgcagtcg tagtatctgg tattaatttg
    25381 tcccatgaaa aactgcctaa tgttccggtt ggtacgatcc caactttgat ccacgcagca
    25441 aaccctaata agagcatcat cgtcaaaggc acgtacaatc ctaaccaaac accgactttt
    25501 ccgccgattt ttgccatatc aaatttcaaa ttcaaaagag tgatgatcca ataaacgact
    25561 aaaatgacaa ctaatgtaaa tttgctgttc aaaccaagcg gcacattatc gataatatta
    25621 ccaaacaacg gagcgagtgc tgaagcgacc atcaccatcc caggaaacat ttggacccat
    25681 aacagccatg agacaacgaa cccccatttt tttcctagag cattcgatac ccaaagttct
    25741 ggaccacctt tttggggaaa cattccagca aattcaccag agatcaatga gattggaaaa
    25801 gcgtacaata atactgcaac cagcatgtaa aaaatcatcg tccagctcgt tgctgctaca
    25861 tctggaatat ttcgtacact tgctacaagg gcgcaggtca ttccgataaa ggtcatggtc
    25921 gtgatttgct ttgatgaacc agtatctttc atctttattc cttctttcaa tagcgacata
    25981 tgtttttgtt ttcttttatt tttgttcaag aacagaaatg acaaggtttc atcgattcag
    26041 tacaaaaccc agtatcaccg aaacatccat tgaacgatct tagtgaactg ggtttgatac
    26101 atgaaacgaa cgatccaact tatagaacgt tttataaaaa agaatatctt ggcgaggaat
    26161 atatgaaaaa cagggggaaa tacaattgtt ttgatgaagc aaataattgt ctgttctcca
    26221 tatgtctctt gttattcttt cacacaaaaa tcagttctat tggatgattt cgtttagttt
    26281 cttttccatt gctttggcga ttttttttgt ataaaaattg aaaattcgat catcattcaa
    26341 ataaggagtc atgatagccg cacgtagcag agtgattttc tgttcttttt tccattcttc
    26401 tttcgttaga cccattcttt caacaaaacg aatcggagaa tcaccgtaat cttcttgtgt
    26461 aaatgtagta tgagaagtaa taaaccgttc accataaaca tcgccatcca tataagaaga
    26521 ccgatcgaac attttttcat ttaattcatt gatagcgttc aaatctgtac agccttgttc
    26581 tttgaatacc caattgacca tattaaaatc aggatgattc aatggataga cttcgattgt
    26641 ttttccttta acagtaaaag tcagatgatc gagaaattct ctgaatcttt gagctgcctc
    26701 gattgaagcc ccaatcaatt gaccatatcc ggtcacattc aatggcaata cacgatgtgc
    26761 tgtccagact gcggcggcgg ttgctccagc ttttgatcct tccaaaatat aagctcctaa
    26821 catatctgga gcctttactg atttttcaaa cacatatggc gcgaaataag aaatgatatt
    26881 tctcatattt ttatgtttga ttacgattcc tccagcagca taagggacat agcccatctt
    26941 atgaggatct atcgtgacag aatccgcttc tgaaatagct ctgaaccctt catatacctc
    27001 tttgtcaatc gttacgcagt ggtggaaaac atgatgttct tcaaagaatt cagctaagga
    27061 agcatacggc acaaattctc ctgcttcgtt tagaaacagt gaacgcgcat agccgccata
    27121 tgcggcatca acatgtaaat agaaatagat cccttcatct tttaaccttt ctcttaattg
    27181 gacgatttta tctacactgt ctacttgacc ttcttctgtc gttcctacga cagcaaccac
    27241 acctagaatt gggatttttt gtccagctaa ttcacgaatt gttttttcca aagcattaat
    27301 atccattcga taatcctctt gaactgggat agcaaccatc tgatctaagc cgactccgca
    27361 aatatctaac gccttcatcc aagaatagtg ttttgtttga gggaccagcc atttccctaa
    27421 tctttggata ttttttccgc tacgggaaga agctgctttg acctcatcca tttcttcgtc
    27481 cgtaaagctt tcggtcattt ccaaaatttc ttcaacagat aaattcaaaa gttcccactc
    27541 agacattttt ttcacttttt caggcagcac ttccttgaca gctaaaggaa tcgatttgat
    27601 gcaacgggcg taccataatc cttcaagatt agcaatagaa ccatctgctg caatgtgtcc
    27661 ccatccgtct gtcatattga ataatgaagc aaaatcctgt cctacttctg cttccatttg
    27721 cgaagttgcc atagaagatt ccaatgcaac attatttggg ttccacagca tcgcatagtt
    27781 gtaagctaat aaagcaggca tcaatgtttc agcattcatt tgtccccagt aacggcctgc
    27841 tgaatgccat gggatcgatc ctgcgcgtaa tcgctgactg acttcatcca agacctctat
    27901 catatggtcg cgggtggcta gataatctgg agtgagctta tctccttcgc taattgcagg
    27961 catatctgaa ggaatataat tttctctcca tccaagatgt tcatctacca tcttgttcaa
    28021 caacatttta taaactggtc cattttcagc tttgtcgcct ataaagacgg ccttgatatc
    28081 catatctttc atttttcttc cctccatatt ttgttggtca atgtcttgca gatacatgtg
    28141 tttatctgta acacctcttc actatagcgc caaaaatgag aaattttttt ttataacatc
    28201 aaatccacgt agatttttac aaacaaaaat aaataaagtc ggatttttct gataattcaa
    28261 cgcaaaaaag cgaacaattt ttcaataaat cagtcttcta aaatagataa cctaaaaaaa
    28321 gcgaac
//
LOCUS       DAZUMB010000024        28067 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000024 DAZUMB010000000
VERSION     DAZUMB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.024"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACG80L_000882"
     CDS             <1..649
                     /locus_tag="ACG80L_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023158.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACG80L_000883"
     CDS             708..1148
                     /locus_tag="ACG80L_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023159.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACG80L_000884"
     CDS             1160..1828
                     /locus_tag="ACG80L_000884"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5023160.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACG80L_000885"
     CDS             1892..2515
                     /locus_tag="ACG80L_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS5023161.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACG80L_000886"
     CDS             2571..2957
                     /locus_tag="ACG80L_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS5023162.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACG80L_000887"
     CDS             3170..4561
                     /locus_tag="ACG80L_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS5023163.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="ACG80L_000888"
     CDS             5172..5540
                     /locus_tag="ACG80L_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS5023164.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="ACG80L_000889"
     CDS             5720..6661
                     /locus_tag="ACG80L_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS5023165.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="ACG80L_000890"
     CDS             6730..7461
                     /locus_tag="ACG80L_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5023166.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="ACG80L_000891"
     CDS             7605..9785
                     /locus_tag="ACG80L_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS5023167.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="ACG80L_000892"
     CDS             9805..10287
                     /locus_tag="ACG80L_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS5023168.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="ACG80L_000893"
     CDS             10303..11064
                     /locus_tag="ACG80L_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5023169.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /gene="murC"
                     /locus_tag="ACG80L_000894"
     CDS             11195..12529
                     /gene="murC"
                     /locus_tag="ACG80L_000894"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS5023170.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="ACG80L_000895"
     CDS             12836..13321
                     /locus_tag="ACG80L_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS5023171.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="ACG80L_000896"
     CDS             13314..13997
                     /locus_tag="ACG80L_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS5023172.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="ACG80L_000897"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="ACG80L_000897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS5023173.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="ACG80L_000898"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="ACG80L_000898"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS5023174.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /gene="coaE"
                     /locus_tag="ACG80L_000899"
     CDS             17613..18242
                     /gene="coaE"
                     /locus_tag="ACG80L_000899"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS5023175.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="ACG80L_000900"
     CDS             18294..18626
                     /locus_tag="ACG80L_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5023176.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="ACG80L_000901"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="ACG80L_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS5023177.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="ACG80L_000902"
     CDS             19452..20825
                     /locus_tag="ACG80L_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5023178.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="ACG80L_000903"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="ACG80L_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS5023179.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="ACG80L_000904"
     CDS             21880..22320
                     /locus_tag="ACG80L_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS5023180.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="ACG80L_000905"
     CDS             22464..23165
                     /locus_tag="ACG80L_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS5023181.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="ACG80L_000906"
     CDS             23162..24283
                     /locus_tag="ACG80L_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS5023182.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="ACG80L_000907"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="ACG80L_000907"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS5023183.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="ACG80L_000908"
     CDS             25662..26570
                     /locus_tag="ACG80L_000908"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5023184.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="ACG80L_000909"
     CDS             26605..26877
                     /locus_tag="ACG80L_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS5023185.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="ACG80L_000910"
     CDS             26888..27934
                     /locus_tag="ACG80L_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS5023186.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9429 a         4850 c         6109 g         7679 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
    28021 tatacataca atcaattttg tttttttaag atttatataa gtcgaga
//
LOCUS       DAZUMB010000025        27706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000025 DAZUMB010000000
VERSION     DAZUMB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.025"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(253..1197)
                     /locus_tag="ACG80L_000911"
     CDS             complement(253..1197)
                     /locus_tag="ACG80L_000911"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5023187.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1493..2437)
                     /locus_tag="ACG80L_000912"
     CDS             complement(1493..2437)
                     /locus_tag="ACG80L_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5023188.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2658..3872
                     /locus_tag="ACG80L_000913"
     CDS             2658..3872
                     /locus_tag="ACG80L_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5023189.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3894..5909
                     /locus_tag="ACG80L_000914"
     CDS             3894..5909
                     /locus_tag="ACG80L_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS5023190.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5992..7365)
                     /locus_tag="ACG80L_000915"
     CDS             complement(5992..7365)
                     /locus_tag="ACG80L_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5023191.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7391..7675)
                     /locus_tag="ACG80L_000916"
     CDS             complement(7391..7675)
                     /locus_tag="ACG80L_000916"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5023192.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7677..9692)
                     /locus_tag="ACG80L_000917"
     CDS             complement(7677..9692)
                     /locus_tag="ACG80L_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5023193.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9871..10173)
                     /locus_tag="ACG80L_000918"
     CDS             complement(9871..10173)
                     /locus_tag="ACG80L_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS5023194.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10262..11293)
                     /locus_tag="ACG80L_000919"
     CDS             complement(10262..11293)
                     /locus_tag="ACG80L_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5023195.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11500..12375
                     /locus_tag="ACG80L_000920"
     CDS             11500..12375
                     /locus_tag="ACG80L_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5023196.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12527..13369)
                     /locus_tag="ACG80L_000921"
     CDS             complement(12527..13369)
                     /locus_tag="ACG80L_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5023197.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13461..15338)
                     /locus_tag="ACG80L_000922"
     CDS             complement(13461..15338)
                     /locus_tag="ACG80L_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5023198.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15335..16756)
                     /locus_tag="ACG80L_000923"
     CDS             complement(15335..16756)
                     /locus_tag="ACG80L_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5023199.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16759..20148)
                     /locus_tag="ACG80L_000924"
     CDS             complement(16759..20148)
                     /locus_tag="ACG80L_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5023200.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20405..21796
                     /locus_tag="ACG80L_000925"
     CDS             20405..21796
                     /locus_tag="ACG80L_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5023201.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21869..22516)
                     /locus_tag="ACG80L_000926"
     CDS             complement(21869..22516)
                     /locus_tag="ACG80L_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5023202.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22524..23210)
                     /locus_tag="ACG80L_000927"
     CDS             complement(22524..23210)
                     /locus_tag="ACG80L_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023203.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23216..24289)
                     /locus_tag="ACG80L_000928"
     CDS             complement(23216..24289)
                     /locus_tag="ACG80L_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023204.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24511..25185
                     /locus_tag="ACG80L_000929"
     CDS             24511..25185
                     /locus_tag="ACG80L_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5023205.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25190..26530
                     /locus_tag="ACG80L_000930"
     CDS             25190..26530
                     /locus_tag="ACG80L_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5023206.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26679..27665)
                     /locus_tag="ACG80L_000931"
     CDS             complement(26679..27665)
                     /locus_tag="ACG80L_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS5023207.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8071 a         5241 c         5036 g         9358 t
ORIGIN      
        1 tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc acagataaac
       61 attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc atatgtacgg
      121 atacattttc ggaccgatta attttaacca ttctgtttat ttgcttacct attagatatc
      181 aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt tttagttcat
      241 attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg ttgatttgct
      301 gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa caccattttc
      361 gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac cagttaaata
      421 agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga tacgtgcagt
      481 actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat aagcagcatt
      541 acggacttta tcagcaatca catctaattc atcttgggca atacgatggt ctttttctac
      601 aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa cttctgaatc
      661 accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta atttcaatgc
      721 gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa cacgtgatgt
      781 tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt ttgaagcaac
      841 aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc gcatgattga
      901 cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga taccagctgt
      961 gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata cagccacatt
     1021 ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg ctttttcttg
     1081 attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg cataagcgat
     1141 acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt ttttcatgaa
     1201 taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc ttgtttattc
     1261 attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga aacgaattcc
     1321 tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga taatacaacc
     1381 aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata ataagacagc
     1441 aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct tcttaatgtt
     1501 tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg aataaagaag
     1561 tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc gtttcttgaa
     1621 tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag gaagcatcaa
     1681 aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga gctgctgttt
     1741 cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta agcatctcat
     1801 tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga gtcaattcga
     1861 tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat gtatcaaaat
     1921 aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg ccgcgaaaat
     1981 gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa gaagaaagta
     2041 gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct tcttttaata
     2101 agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta gcttctttat
     2161 caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata atttgtaaac
     2221 atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg ggaacttccg
     2281 catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc cctcggtgga
     2341 tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc attccttgtt
     2401 cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa ttcagctagt
     2461 tccaattgac tccctccttt ttgtaagcgg tttctttcgc ttattagttc acgctgtact
     2521 gctattatat gcaaagctat ttttggtttc ataaagactt tctatggttg ggacatgaac
     2581 ttcctgtgat tccgctattg agagaataac cacttcatac tatatttttg ataagcgctt
     2641 acaaaaatga aaggaacatg cttatgaata aaaagatgat gaaattttct ctgctgtcag
     2701 tctctcttat tttgacttct gctggtgcag tcacgtctgc aattcccagt atgtcagcta
     2761 cttatgccac gcgttcttta tctgctgttg aattattaac gactgctccc tcttttatgg
     2821 tcactatttt tgttttgatt agtagtttta ttgctaggaa aatcggacaa aaacaaactg
     2881 tgatcatcgg attagtgatt gcatcgattt gtggtatcct ccccctcttc tcacaaagtt
     2941 acagtattgt cttagcttca agagctggac taggtattgg ctttggattg atcaattctt
     3001 tggcagtcag catgattgct gccttttatc acggagatga acgagcctct atgattggtt
     3061 ttcaaagtgc atttcaagga tttggtgcag ctttaatgac atttgttgca ggacagctag
     3121 tacagttcgg ttggcaatat acctttttag tctacgccat cagcttacct attcttgtct
     3181 tgtttgtttt gtttgttcct acacctgaaa caactgaact tcaatcgcat ggcacagccc
     3241 caacaagaca agtaatcaaa acgaatccta aaatgattag ttatggtatt tttctcttgc
     3301 tggtcatcgt ggtatataat gctgtatcga ttaaattagc cacagtcctg acgacttatt
     3361 ctctaggaaa cgaaactaat gcagcgaata tcttaacaat catgcaattg gcaagtatgg
     3421 ctgccggagt ttgttttggc tggcttcaga caaaagcaca tcgctatatg cttacacttt
     3481 ctttactact gatgggaatc ggctttgtat tgattgccac tgtgataaac ctgtatgtga
     3541 ttgggtttgg tgcgatccta acaggaattt ctttttcgtt attcattcct tacttattca
     3601 accaagtaag tatccaagcg cccaagtacg cggagagttt caatacttca atcttattgg
     3661 ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc tcttttgctt
     3721 ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg ttgattagcg
     3781 cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata agagtagatg
     3841 aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa
     3901 atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc cgccatggac
     3961 tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac tggtacatgg
     4021 atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa ccttccatgt
     4081 ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa catccatgtg
     4141 gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta ggacatacat
     4201 cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt ggctggacag
     4261 caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc gatatcgcaa
     4321 aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact gaaacggaga
     4381 ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta gaactaatat
     4441 ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat gtagaagcta
     4501 tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg aaagctcgta
     4561 aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt caacttcaag
     4621 tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat tcttctttac
     4681 tgaatcagat ctggtcagta tcagaagaag ccttcaagtt atgtagtgat ttgtttttta
     4741 tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa tcattcatga
     4801 tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta gcattaagag
     4861 gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc ttgtggttga
     4921 ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc atcgtctacc
     4981 ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta gggtttattt
     5041 atgggagagc aaaagattgg atatttattg attggtctga aatggataaa gaaggaacgc
     5101 ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact tgcggagata
     5161 tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat caagaaaaat
     5221 atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt ggcgctttta
     5281 tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt
     5341 tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc ttattgaatg
     5401 acactatcac acagattact actccatatt tcaaattcta tgaacaagat gctttatgca
     5461 aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga gggatgttat
     5521 ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga aatgaaaagt
     5581 acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa
     5641 tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt tatgaagaat
     5701 ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta cctatcaaag
     5761 atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct gatagaacag
     5821 gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag ccacttgtct
     5881 taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca tatcagaaga
     5941 ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg cttaattctg
     6001 ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata atacgacaag
     6061 taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc ctagtttccc
     6121 aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag cgattttaag
     6181 atctcctaat actggaagga taaataatgg cagccaacta atcaataagc tattgaaagc
     6241 agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac cagaagcagc
     6301 tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca agcctatcat
     6361 agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta atacagcatt
     6421 aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg gtacgagttt
     6481 tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa ccccttgaag
     6541 gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt
     6601 gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag caagtgcgac
     6661 atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa agctgaaact
     6721 tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca
     6781 tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat tgtttccagt
     6841 gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg ttccttcgaa
     6901 gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac
     6961 tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga atttatatct
     7021 tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca aagcagtagg
     7081 tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa ctactccttg
     7141 agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac tgattaaacc
     7201 agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt ggatcacttc
     7261 tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa cgaggatgga
     7321 gggctgactg acgattgaca caaacaaatt caacaattta tccattgata gttcctccct
     7381 tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact tcttcgctgt
     7441 caatgatcga ttgcaaaggt acgagctttt catcaggtat agaagttagc gtatctttca
     7501 aagaatgctc aatgaagaaa taatcagcca tatcgctaga tacacttcca acatccgtat
     7561 gacttacttc aaactcgtcc acatctgctc caagatctgc aagtgcttgt ttgatgttca
     7621 tctctaccat aaaacttgtt cctaatccac tttgacatac tgcaaaaatt ttcatatcat
     7681 tcgtctcctt ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg taataaatct
     7741 tgaagatttt gtttattaga aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc
     7801 tcattatcga tagcagctaa acagatgaac aaactaatgg gatgggctgg attatcaagc
     7861 agatccacag gttgttgaag tttcagcaaa gacatcccta cttcgtttac gccatcttct
     7921 ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc atactgctcc
     7981 acgcgctcga tcatagcttt gatataactt tcttctattt tattttctga taatagcggc
     8041 tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt gatccgataa ttggatcatt
     8101 tcttctgtta ataattcaga tagcatagga cgttgatcct ccttcttttc taaaagtttg
     8161 ttcattttac gatttaatac ttgatagagc ttttcttcgt ctacgccttc ttttaattcg
     8221 atataaggca gtagcgccga caacaattct tgggcagaag gcattgaaaa tcctggtatc
     8281 agccaatctc tttgtacttg attgatcagc tgattttttt ccaagtcagt catgattgga
     8341 tgcagaacat agagagactt tttcttatca gtaagtgtga gaggtacagt agaaaaaata
     8401 atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa
     8461 aagatcatcg ggaataattg ctgaagttca ctttttaaga tcaatgaaga gctaatgcca
     8521 cttggacaga cgatcacggc tcttattttt ctatttcttt cttctgcgtc cgcttttctg
     8581 atctctccgc caaatagtat agtgaaaaaa ccaatctctt cttcagggat gctcttccct
     8641 attcgtcttt ctaacggaag taatgctttt cttgtcattt cgtagatgga agcatattga
     8701 tgcttaattt gatcgatcat cacattaggt aaataaaaag aataggaaat acgaaaatag
     8761 gcagggacca agtgataaaa gacattcatc aataattcac gaaacgaagc aaattctacg
     8821 gccatcagga attccatgcg atggatgact tctgaagaac attccagcaa aaaatcaagt
     8881 gcaggctcct ggacatctcc ttcaacgatc gatgctaagc aaagagagaa aaatataatc
     8941 tccgaatttt gatgttcgga atagccaagg ccagtcaata cttgtcgact aatgtcagtg
     9001 actgcaagag aagccaacag ttcagcatct tttaccagtt ccatggatgt ttttccaatt
     9061 cgaatggttg tcaaactcaa ataataagct aattcatcca ttcgatttgg tgcgaatcga
     9121 agatctgata gtgaaatcgt tttatctagc aaatctctaa ctttggcgta ataatcaatg
     9181 gaaagggttg cggttaaata cgtgagccca aatcttccag caagtgactg catcaaacga
     9241 gcagtgcgat tttttgcttc tcttcttaat attgcttctg accctagcag cctaaaacca
     9301 gttttcctag tatacgacag ctgacaatct tccacttcta attcatttct tatctttttg
     9361 atatctgaca ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa
     9421 acagacgttg cagtcgaata aaccatcaaa taaagaagat ctcttcgctc ttgttcggta
     9481 tacacagctt caaatcgagt aatatccaaa aatttttctg cccagtcgat tgctcggaat
     9541 ttctttatac ggatacgggt atcatccaca tattcggcaa aaggagttaa ttgtttgatc
     9601 gcctcgcttg tatttacttc gatgatctct gctgcttttt ctagactgat ttctcttggt
     9661 gttttcagga gataactaat tttgtagttc acagcgctgt tcacctccta cttatattct
     9721 agtatggtag cgcttttaaa tcgaggggtg tttggtacaa cttacagttg gatttttttt
     9781 acgactgtac cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt
     9841 ctcctgcttt tatgaattga tttcgtccga tcaatgatga tcatgtttat tttttgaggt
     9901 cagttcttca aatttttctt tgacatcttc aaccgctttt tcgatatctt tttcaaagtg
     9961 attgggctca ttaggctttg tttcctcttc tacagaattt gaaccagata aagctgtttc
    10021 tttattttct gtttcaccag agtttgtgcc agctaatgat tcttctttgt cttctgtttc
    10081 accagaatta gttcctgcta aagccacttc ttctgtttta ggggccattg aacctcttaa
    10141 tcgtttgtgt ttttcaccgt cataatttgt catgttggtt cctcctagta gttctatact
    10201 ttttcatcgt acaataagac agtttatgcg tcaaaaaaca aacccgattc ttacaaatat
    10261 gtcatatttt tccttctgat tcttgaatgc ttttgattcc ttcttccaaa attttcattt
    10321 gtaagattgt ttcttcatct ttgatgactt tttctgcgcc attaactaat gtctcgtaaa
    10381 tcccttcgta gacacggctg taatcaccaa cctcggaaat tactttttct tcatgataac
    10441 tcccttgttc atctatatag gtcaatacac cataatgttc gggcaaatcg ataccaaagt
    10501 cttcatgatc aggcatgtaa aacattttca gatgttcttc ttgtcgatct tttgtttgtt
    10561 tcacgaacat cccttttttt ccgtaaacga cgaaacttgg acgttctttg atgcggaaat
    10621 aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg aagtaatcat
    10681 tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct ggttcgccga
    10741 aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag ctgttcgcag
    10801 tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa tgcatttcta
    10861 cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc aagaagtcag
    10921 aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt gctaatgcaa
    10981 ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt acattcttac
    11041 catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta gtgatgacaa
    11101 ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat tcgatgtctt
    11161 cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg attttgttcg
    11221 taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg ccaagataag
    11281 cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc ctagaaaagc
    11341 gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac ttgttatcat
    11401 tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata gtagcggaac
    11461 caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga agaaaaacta
    11521 acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat ccgtaggaat
    11581 atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta tacttcccat
    11641 tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga attccgtttc
    11701 gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat tgatcctaat
    11761 tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc ggatttatcg
    11821 atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga tcaaatcggt
    11881 aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc tcaaatcacg
    11941 gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat ggctgaagaa
    12001 tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc catttttatt
    12061 agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa gcatgtagag
    12121 gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca ggctgatatg
    12181 tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt ctcctctcaa
    12241 attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca aaattttgaa
    12301 gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct tcttaaagaa
    12361 tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg tttgataggc
    12421 tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct cagcctgttt
    12481 gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa cttctgtttc
    12541 ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt atacatgcag
    12601 ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact tctttttgaa
    12661 cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga ctgttgacca
    12721 tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt ctcagatctt
    12781 ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt gtccgtccat
    12841 tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg aacaactttg
    12901 cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc cccgtaggat
    12961 aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc gtttcatcaa
    13021 aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta tgtttctcaa
    13081 aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct gcacctggat
    13141 ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg attttagcca
    13201 tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc tgctgatcca
    13261 gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt cctgagagaa
    13321 gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt tgtgttctcc
    13381 ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg ctgctccttt
    13441 ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa gtacagtcca
    13501 gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt accgcctgtt
    13561 gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa ctgtcctgct
    13621 gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg aacatatttt
    13681 tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt cacatcgatt
    13741 aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac ccagctgacg
    13801 gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg atcacgaacg
    13861 acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac tttaacaaat
    13921 cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt atgcccgtta
    13981 tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt caaatgcata
    14041 tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt cggatcaact
    14101 gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc gatttcttta
    14161 tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa tgttaaagca
    14221 gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc ttctttatca
    14281 gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc gattgcgaca
    14341 tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt tttgatggtc
    14401 ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt ttgcttttca
    14461 atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc aacttctaaa
    14521 tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt gttgccgatg
    14581 tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt ccaagtgtac
    14641 aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc ttctttagaa
    14701 ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt tgtaactttc
    14761 aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc tagttcttct
    14821 gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg gaaagctaaa
    14881 accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt gtagactgca
    14941 tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc atctgtcgtt
    15001 cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc tgcatccacg
    15061 gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac tgaaaatgaa
    15121 atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct gttttggatt
    15181 aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc tgctgtctcc
    15241 tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat aaggaaaagg
    15301 actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct gttctttttt
    15361 tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct aaaagtgtat
    15421 ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta ttgactactt
    15481 tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta aacggaattt
    15541 ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga taacttcctg
    15601 ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga agcgcatctg
    15661 attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt cctgctttgt
    15721 ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc tcgattttga
    15781 caaattgctt actccctgta tatacagaga ctttctttgt actgacacca aaaccaaaat
    15841 ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt agctgcatct
    15901 gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc gaaaataaaa
    15961 catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt tctccattcg
    16021 atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta tctgacaatt
    16081 cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa taagtgattt
    16141 tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa ggtggatcag
    16201 ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt tgattgtttt
    16261 gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg acttctggta
    16321 ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta gcggccagag
    16381 aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct gcggtaaaca
    16441 taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc gttttgccct
    16501 tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg acaccattta
    16561 atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga tttttcggca
    16621 gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct gtttctgttt
    16681 ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac atactaagcc
    16741 aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa gataccaaat
    16801 agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa ttcctcccgt
    16861 ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt tgtttttggc
    16921 tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc catcttcgtg
    16981 tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa aagtttcggt
    17041 cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt cttgtccatc
    17101 ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc cttttaatgt
    17161 ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc gattttgatg
    17221 agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa tgatttttcc
    17281 atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg caggaccagc
    17341 tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa gacctttttc
    17401 attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt tctgtaggga
    17461 aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat acttacgtac
    17521 tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat catcggaagt
    17581 agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga tttcccaagt
    17641 agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg tataggtcat
    17701 ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat gatctgttag
    17761 taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat ccttttcacc
    17821 tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag tatttttcca
    17881 tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt tgattgtttg
    17941 gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct cttgataact
    18001 ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat ttgttgtatc
    18061 tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat ttgtcgtatg
    18121 ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt tgtcaaattc
    18181 ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg gtataccgaa
    18241 ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt tcatttgata
    18301 ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt aatggatttg
    18361 gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc cttctatgga
    18421 aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa gtgtaccagg
    18481 ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta cagttgctga
    18541 gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat cagctgattc
    18601 atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag actctacttc
    18661 tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac catgtatttc
    18721 gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg tactgtcgat
    18781 tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta gagaagtatt
    18841 tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc ttgattgtgc
    18901 gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca aaacgatcac
    18961 ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg ctttttgtgt
    19021 aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt tcactgaatc
    19081 aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg agtagccgac
    19141 atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga caaaacgatc
    19201 gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg accagtctac
    19261 gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag cattcaaata
    19321 aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg catagtctgc
    19381 ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag gatctccatt
    19441 ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat gatcaagaac
    19501 atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat catctgttgt
    19561 atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg ataatgtagg
    19621 caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt ctatttcgtc
    19681 tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa taatgtcata
    19741 ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg gatcgattgg
    19801 aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa ttttcccgct
    19861 atttacctga taagcatagc catctccttc ttttctttcc acatcactta agtaattttc
    19921 gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat ttagcttgat
    19981 gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg tttctaaaac
    20041 tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg ataaactcaa
    20101 tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt ttcccctcct
    20161 ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac aggcgttaca
    20221 taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg gaatcacacc
    20281 aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat gagcgtttca
    20341 ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaag gaaactatag
    20401 aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga ctaaaaaaga
    20461 acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg aagagatcaa
    20521 aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt atcgtcttga
    20581 acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa gttctacaaa
    20641 cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt attattgtga
    20701 aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca atcaaatttt
    20761 agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta gccttaaaag
    20821 tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg gaagacgcac
    20881 gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga ataattgtca
    20941 gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta tgtattttgt
    21001 tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg gagactctta
    21061 tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc tcttaggaaa
    21121 attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga ttttttcaga
    21181 attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata tgtcctactg
    21241 ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc aattattaac
    21301 gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga cgatccgtgc
    21361 aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg gatttcgtgc
    21421 agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt tatctagcta
    21481 tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg ataaaaaaat
    21541 actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat atatcagtga
    21601 tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag atcagctgtc
    21661 gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt tgaatgccca
    21721 tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag aactcatccg
    21781 cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg ataaaaattt
    21841 caggacaaat acacatagct gtttcttttt attgtattcc ttcagcaatc caacgagatt
    21901 tatagcgaaa gtcttttaac aactcttctt ttgtccaatt atttgcaatg agatccgtca
    21961 aagtgttcat tacgagggtc atggaaaagt ataggaaact aactgcttct tccgacgatt
    22021 gaatggagaa atgattgatt cttaataatt ccagccattt ttcgttcatt ggagaatcag
    22081 ccgatagttc tctacgtttg gttgtctgct gatcatttcg ctgggttaac aagtataacc
    22141 tttgccaata gatactttcg tgatctaagt cgcagcacca ctctaaatat ttttcgatcc
    22201 ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa gatatcttga tggactatgt
    22261 gagaactgct tttgatcgta tacaaataag catcttccaa atcttgaaaa tatttataaa
    22321 aagcaccgcg cgacatcccc atcttgtcaa cgatttctga gactttgact tggctgatgt
    22381 gctgtgagca aaatatctcc agtaataggt catcgattct ctgtttcttt gcgttcgata
    22441 aattgaaaaa agtttctttt ggcatccaca tctctccttt atctctgatt atacgtggat
    22501 agcccttcat tgtcatgaag gattcattcc ttctttttct cttcttctgt caagtaccca
    22561 tcttccattc gataaacgcg atcactccat tgaatcatac ggacatcatg agtaaccata
    22621 atagtcgctt tttgtttctc atgagcttct ttaactagta atttgacgac ttcatatgca
    22681 tgatctgtat ccaaactagc tgttggttcg tctgctaaga tcaagcttgg ctcattatat
    22741 aaagcgcgag caattgccac tctctgtctt tctccaccag aaaggtcttt aggataacta
    22801 tttttaagtg actggatatc taaagattgc agcaaggaat cgatttttgt tgaatcttct
    22861 ttttttgaag taacttggtc aactagttca aattgttcct ttaccgttaa aaaaggaatc
    22921 aaattagagc tttggagaat aaatcctatt tctttaaagc gtaaatctgc tcgttttttt
    22981 tctggaagct cagttagtga gtgaccgttg atcactattt ctccggaagt cggtgtttgc
    23041 aatcctcctg aaatggtaag gaaagtactt ttacctgaac cagaaggacc aatgatgctg
    23101 acgaattctc cttcctttac ataaaaatca attgctttca aagctgagac ctttgtatga
    23161 ccagaaccaa atgttttggt aacttttttc atcgttaata tttttttcat ttttattaac
    23221 ctcctattgc tgtaattggg tctactttgg ttatcgtacg tatcgaaaac aatccgccta
    23281 gcattgcgac aatgatcaaa atcccactat atagcaacca ttgtgaccaa aaaacagcaa
    23341 aaggcatcgc gcttggtaaa atcagactag tcaaataagc aaacaaaaga gcaagcagaa
    23401 cgcctaccac cccaacaata aaggactgtg ctactaaaga tttagcgatg aagaaatttt
    23461 tgatcccttg tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc atcacgtaca
    23521 tgaaaatacc gaccacggca gcaaccacta agaacaaaaa gtatatcatc gcatttaagg
    23581 tcatattttg ggcagaatag ccaggaatac tttcaatgaa tgtcccaata gttaataact
    23641 gtagaccctt cgatgctttt tctttcgaaa ttttagctga atcctttatt acgatcccat
    23701 tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa gcagtccacg
    23761 tatctaggga cgcatagatg acgggagaaa ctgtgtaata agtttcagga aagataccga
    23821 caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt atctccaatt ttatagcctg
    23881 aatcagccag attttgagag atgatgattt cattctttgt tttaaattca tgccctttcg
    23941 tgagttttgg taataaaaat gcttctttcg ttgtgccgaa aactgtaaca ttttctttgt
    24001 tttttccttt gattgctccg ctgtacaatc cgattggtgc tttctctcct ccttcaatgt
    24061 atttcagatc tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca tcctccgaca
    24121 aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt aaactcttct gaaagtccat
    24181 tcgctagtcc tgacaacatg aatacgacat acgcaatcaa gaacataata ccgacgatca
    24241 agccatatcg taatttagaa tagcgaattt ctttgatagc tagaaacatt tgtttttcct
    24301 ccctatgata gattgatagt gacaaaatgt cacttgttga atagtgtaac tctaaagtga
    24361 caatttgtca cttgtgttgt ttttttctta tcaataaatt gtctaagaca tctttcgata
    24421 aaaccttaac atgagtcata aaaaatttat ttaagcctta tttatgcttt ccatgataaa
    24481 ctaaagtgac caaatcaatg aggtgggaaa atgattaaac tattagtagt agaagacgat
    24541 aaagtattat ccaataatat aaaagaaata ctcgctgcaa tcggcgagat aacacaagtt
    24601 tatgatggta gtgaaggact gttcgagttg gaaagtggag tttatgatct tgcaatcttg
    24661 gatttgatga tgccggaaat gaatggatat gaagtgttag caaaaatacg gaaagaaaaa
    24721 atacagactc cagtattgat tttgactgca aaagatagtt tggaagataa gattgaagga
    24781 tttgaacgag gtgcagatga ttatctaaca aaacctttct atcgagaaga actgatcatg
    24841 cgcgtgaaag cattattgaa acgctcgcta ggtctctttg atgaaaatgt tctgaagtat
    24901 aaggatatca gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca agttttgccc
    24961 atacaaggaa aagagtttga attgctcgtt tattttttac aaaataaggg aatcattttg
    25021 acaaaagagc agatatttga ccggatctgg ggatttgatt ctgatacgac gattacagtt
    25081 attgaagttt atatgagcaa cttacgaaaa catctgcgaa gtactggatt ggataaagaa
    25141 atcaaaacct tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa tgaatggaga
    25201 tattacacga aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat ttgcttttat
    25261 cttttttctt ttgggattca ttactttaca gatattaaac caatctgcat acagagaaac
    25321 agatctgtca ttagagaaaa taaattttga ttctcgaaca gtacaattag agatacaaag
    25381 atatcagcaa ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca gaacaaacat
    25441 ggaacagcca ttaatggaac catcaaatga ccgttttaac actcaagtga tcttgtggtc
    25501 taaaaatggt gagatattga ataaagcagc tattggtggg cgtttcaacc aaatcaaaga
    25561 attaaaaatg gatacgaaaa atctgaatac catccaaaat attgaattaa atgaagacag
    25621 tggctcatct gccctctctt tccgttcgat tacgaaaaaa gcggagacga atcaagatgg
    25681 cgttgcctac atccaaattt tagaaaatac aaatcaaatc aagaattcat tagcgacttt
    25741 tcgcacgatt atgattttgt gtatgattgt tttttggatt ctctctattg gtatcagtta
    25801 ttacttgtct agtctaagca tgcgccctat tattctttct tggagaagac aaaaagaatt
    25861 tgttgagaat gcttctcatg aattgcgcac accgttaacg attattcaaa atagtttgga
    25921 acatcttttt accaagccta atcatactat tattgaagaa tccgaaagca tcgctcaagc
    25981 attgtcagag acgaggcgcc tcactggatt gacttcggat ttgctgacaa tcgctcggaa
    26041 tgattctgat cagcagctgc tttcaaagca aatgatcaat acacaagaat atatcgaaca
    26101 acttgttaaa ccatttcaag agatggccat catagatgga aaagagttta ttctccataa
    26161 cgatgcaaca ttagaagtac caattgatga aaaaaaaatc catcaagttt tggttatttt
    26221 gctggataat gcattaaaat atacgaatcc agaagataag atcattttgg attcatctgt
    26281 taaccataaa gaatggctga ttcacgtaaa gaatactggc ccttctatca gtgaagaaga
    26341 tcaagcaagg atttttgatc gattttatcg ggaagatcaa tctcgggcga aagagactgg
    26401 cggatacggg cttggcttgg caattgcaaa acaggtaatc gaacagcatc atggacagat
    26461 ttctgtatcc aattatcttc cacaaggagt cgattttaaa gtccaattgc cgttgaagcc
    26521 taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca cagccctttt
    26581 ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcttt
    26641 aagctgacca cttctgtcac aacctcttta gcttacgctt acgatttttt attgcggtct
    26701 tcaatgtgta catcaccgct tgtgtcaata tccaattctt ctcggctaac atcttctgtc
    26761 actttttcaa catcttcatg ttcttcttta tgaatcccaa cttcttcacg aataactgta
    26821 tgtttgctta catctacttg ctcttctttg attgggatag taatcgtgtc atcttccaat
    26881 ttttcgtctg ttccctgtga agattgatct gttactggtt tgcgttcgat gacgatttct
    26941 tcacgtttga ccggtacttc taccgtttca gtatcgttga ccacatgttt gtggatgtcg
    27001 acttctccag ttgtcacatc atgtgtattt acatctaatt tttcttcttt tagttgaatt
    27061 gtatcatcat ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt acccattgta
    27121 tcagtctcgc caggtacgtc catcggatca gttcctggtg tcatattcaa tggatccgtt
    27181 gtattttgaa agcctgtcgt gccacctacg tccattggat ctgttccagt gccagtttct
    27241 aatccagtcc ccattgtgct tgctgggcta acagtatctt gtgtatccat tgtatcttct
    27301 gttgtttctg gctgacgata gccttttact gtgatgacaa attttccatc agaaatatct
    27361 cctctatatt gatataaagg atcattttct ggtgtagccg tctcagaatc ataggtatca
    27421 aaagaaaaag tatcttttat tttttcccat aaagaacggt cttcttcacc atccatccga
    27481 ttttcatctg tttcaacatc tataccagac aatccgtcaa atcctagtct agatgctgct
    27541 tcatcagtag tataaataac gatatcatct cgttcatacc cttcgttacg caaccgttca
    27601 attactgata atgcttcttc cctcgtacta tagcttccga tgaccgttaa atttttctct
    27661 tccatgtaaa ttcctcctag caaatagatt tatgattggt atattg
//
LOCUS       DAZUMB010000026        27568 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000026 DAZUMB010000000
VERSION     DAZUMB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.026"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1210
                     /locus_tag="ACG80L_000932"
     CDS             <1..1210
                     /locus_tag="ACG80L_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5023208.1"
                     /translation="NGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKAENDMKK
                     KIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITISNTKDIF
                     RLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAEILNYIE
                     FNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYT
                     LFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEV
                     VMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRR
                     HPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFE
                     AFQRGLNKCD"
     gene            complement(1298..1690)
                     /gene="rpsI"
                     /locus_tag="ACG80L_000933"
     CDS             complement(1298..1690)
                     /gene="rpsI"
                     /locus_tag="ACG80L_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5023209.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1706..2134)
                     /gene="rplM"
                     /locus_tag="ACG80L_000934"
     CDS             complement(1706..2134)
                     /gene="rplM"
                     /locus_tag="ACG80L_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS5023210.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2668..3135)
                     /locus_tag="ACG80L_000935"
     CDS             complement(2668..3135)
                     /locus_tag="ACG80L_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5023211.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3255..4793)
                     /locus_tag="ACG80L_000936"
     CDS             complement(3255..4793)
                     /locus_tag="ACG80L_000936"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS5023212.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4873..5559
                     /locus_tag="ACG80L_000937"
     CDS             4873..5559
                     /locus_tag="ACG80L_000937"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS5023213.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5760..6287)
                     /gene="msrA"
                     /locus_tag="ACG80L_000938"
     CDS             complement(5760..6287)
                     /gene="msrA"
                     /locus_tag="ACG80L_000938"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5023214.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6371..7048)
                     /gene="pgmB"
                     /locus_tag="ACG80L_000939"
     CDS             complement(6371..7048)
                     /gene="pgmB"
                     /locus_tag="ACG80L_000939"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5023215.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7045..8175)
                     /locus_tag="ACG80L_000940"
     CDS             complement(7045..8175)
                     /locus_tag="ACG80L_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5023216.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8175..10358)
                     /locus_tag="ACG80L_000941"
     CDS             complement(8175..10358)
                     /locus_tag="ACG80L_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS5023217.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10355..11380)
                     /locus_tag="ACG80L_000942"
     CDS             complement(10355..11380)
                     /locus_tag="ACG80L_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5023218.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11425..12252)
                     /locus_tag="ACG80L_000943"
     CDS             complement(11425..12252)
                     /locus_tag="ACG80L_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5023219.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12249..13316)
                     /locus_tag="ACG80L_000944"
     CDS             complement(12249..13316)
                     /locus_tag="ACG80L_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS5023220.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13342..14178)
                     /locus_tag="ACG80L_000945"
     CDS             complement(13342..14178)
                     /locus_tag="ACG80L_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5023221.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14175..15044)
                     /locus_tag="ACG80L_000946"
     CDS             complement(14175..15044)
                     /locus_tag="ACG80L_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5023222.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15114..16397)
                     /locus_tag="ACG80L_000947"
     CDS             complement(15114..16397)
                     /locus_tag="ACG80L_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5023223.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16411..18096)
                     /locus_tag="ACG80L_000948"
     CDS             complement(16411..18096)
                     /locus_tag="ACG80L_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS5023224.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18371..19396
                     /locus_tag="ACG80L_000949"
     CDS             18371..19396
                     /locus_tag="ACG80L_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5023225.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19991..23644)
                     /gene="rpoC"
                     /locus_tag="ACG80L_000950"
     CDS             complement(19991..23644)
                     /gene="rpoC"
                     /locus_tag="ACG80L_000950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS5023226.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23723..27340)
                     /gene="rpoB"
                     /locus_tag="ACG80L_000951"
     CDS             complement(23723..27340)
                     /gene="rpoB"
                     /locus_tag="ACG80L_000951"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5023227.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
BASE COUNT         7671 a         5917 c         5112 g         8868 t
ORIGIN      
        1 aaatgggaaa tataaaacta atctgagata tccaaaaagg tttaaagaaa taacaggaat
       61 tgcatcagaa aagtatcaaa aaacatttcc aaataggcaa ttggcgatta aagccgaaaa
      121 tgatatgaag aagaaaatag aaaaagtttt gagagaggag aatgcaaact cattagaatt
      181 aaaaggaaaa ataacgttta aaaaatttta cgaatctaaa tggcttccta gatatgaatt
      241 aggtcaaaca attagatcaa acaggcctcc tagtgatata accattagta atacaaaaga
      301 tatatttcgg ttgcatattt tacctatgtt tggtgaatac gcaatgaatt atctaaatat
      361 caatactgag ataatatcag atgaattaac caaaaaatcg aaagaatatg caaatataaa
      421 aatcataaaa ggatatgttc gttccatgtt cgatattgca gaaatactaa attatatcga
      481 atttaatcga actactaaaa ttattcaaag tatcactgca cctaaaaaaa atgctttaga
      541 agaaaaaaga atacaagaag gtaagcaagc actatcatca aaagaactta ctaattggat
      601 agaagcagta aatgatgatt ttaataatca tttacttact tttcatgatt acacactttt
      661 tatgattacc ctgtatcttg gtgatagaaa aagtgagact tacgctttgc aatggaaata
      721 tattgatttt gaaaaacaaa cggtccgtct taaacatact ttagataaat atcaaagaaa
      781 aaagtttaca aaaggtcgaa aagataccgt cattcaagta ccagaagttg taatgacatt
      841 gctaagtgaa tggaaatcgg tacaagctga tcaattgttg aaattaaaaa ttaagcaaac
      901 gttagatcag tatctcttta cttatactaa accatctgga gaagtaaatt gtcctgttca
      961 tgccgattac ctaaactatc gaatcaattc aattaaaaga aggcatcctg acttagttca
     1021 tttatctcca cacaaactcc gtcatactta tgcaactata gctagacaag gtggagcaga
     1081 tatgaatcaa atttcaaatg ctttaacaca ctcagatatt tctacaacaa agatatatgt
     1141 gaacacacca gatatagttg ataaggctgt ttttgaagct tttcagagag gattgaataa
     1201 gtgtgattga tatgtgttct taaatgtgtt cttaaaaaca aaagaagcac tttccaaata
     1261 attgaaaagt gccagaaacg ttgatatata aaggatatta acgttttgag aattgtgaag
     1321 ctttacgtgc tttcttaaga cctggttttt tacgttcgac catacgtgcg tcacgagtaa
     1381 gtaagccagc acgtttaaga gctaaacgga agtcaggatc aacttctagc aatgcacgag
     1441 cgataccatg acggatagct cctgattgtc cagcgtatcc tccgccgttt acgttaacaa
     1501 atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt acttcacgca
     1561 agtcagcatg tggaatgtat tcttcaacat cttttttatt tacagtaatt ttgccagttc
     1621 ctggtactag acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg atatattgaa
     1681 cttgtgccaa tgaaattccc tcctattaga ttaagtttgt gatgtctaaa acttctggtt
     1741 gttgtgctgc atgtggatgt tctgctccac cgtatacgtt caattttgta aattgtttgc
     1801 ggcctaaagt gtttttagga agcatacctt ttacagaagt ttcgatcaaa cgacgagaat
     1861 ttttagcacg caattcgcca gcagtgattg atttcaatcc tcctgggtag ttgctgtgac
     1921 ggtaataaat tttgtcagtc gcttttttac ctgttaattt cactttgtct gcattgatga
     1981 cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt tggtttattt ttaccacgta
     2041 gtacagatgc aacaactgtt gaaagacgtc ccaatggaac atcagttgcg tctactacat
     2101 accatttacg ttctacttcg cctggtttgg ccatatatgt tgtacgcacg ttattttcct
     2161 ccaatttaag ttctgatgtt tgacatacac ttcgagtttc cggggctctt tgtggggcaa
     2221 acaataccat ttaacatatt acccgctaat ctagtcaatg tcaacgtttt tctgcttgat
     2281 tgaaaaattt atatgaaact ttacttcttt tctttctttt atataaaagg gtcgtttcct
     2341 gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc cttattttga
     2401 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat aaaacataag
     2461 aagctgtttt ttaaaattgt ttcttgtgac tcggagctga acggcaggct tacaacctct
     2521 tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga
     2581 ggaactattt tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
     2641 tttttcggtc aagttcaaca acagttttta cgcgtcgatc tctggcgctt tgcccaaatg
     2701 agcttggatc atccagatac gtttttctgt ggctgttttg aattcgataa agatatcttg
     2761 tgttggatcg tcgccttctt ctccagaaac ttcgattcct ttttggtaaa gatctgctag
     2821 ataacgatag cctgcaacaa gtgtttccaa acgttctgga atagtacgat cccagcggcc
     2881 aatttcatca gggatttttg tgttatctgc aaattcacgc aatgtagaat aaggtgaacc
     2941 gtctaatgtg atcaagcgtt cggatacttc atctaactga gcgttcaaat catccatgaa
     3001 ctcatccatc attgggtgca atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg
     3061 gtgaacaaca acagacattt ggcttagatc tgcaactaat tgatttaata cttctttggt
     3121 ttgttggaat ttcatctttc ttgcctccta acattttctt acacttaaaa taatagtaga
     3181 ctttcaatga aaagtacaac aaaatcattt acctgagaac gattttcagt tcgctttttt
     3241 atccgtaaag ccaatcattt ttcagaatca gctaattctt ctcgcagttg agcaaattgc
     3301 tgataagtct gtgctaaatg cttttgtgtc gaaacaaata atggatagat tttctgatat
     3361 tttttatgat cagtttcttg tactccaact tgctctttca attctatcaa ttcctgtgct
     3421 tcactgatac tttggatctt tccgatgctt ctccagccta aaatcacagc tcctaatgcg
     3481 gatgcttctg tgacagtagg gaaaacaagc gggtaaccca gtacatcagc catcatctga
     3541 cgaaaaatcg gagattgagc aaacccgcct gtagcacgga tctctgtgag ctgattttcc
     3601 tggcaaactc cttttaaaat ccgataaagg ttgagacaga tcccttctag aatcgccttc
     3661 atcatttctt tttctgtatg ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg
     3721 tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa aaatcagacc attggcaccc
     3781 gctggcacaa aagcgatagc cgaaagcagt gcatcatatg gattttctcc tttttcttta
     3841 gcagattgaa cgacttcctg taaaaatgta tgccctgccc aatcaaagat gccagcacca
     3901 ttgctagtcg ctcctcccac tacccaatgt ttctgatcca gaacataaca aaacgtctcc
     3961 atttctggat ggaactgagg ctgagtagta ataaaacgaa gtgctccgct cgttcctacg
     4021 gtcaaagcgg cgatcgtatc accaaaagca cctaacccta gattagaaag cggcccgtct
     4081 gcccctccca aaacaaatga tatatcattt ggcaaaccta gttctgctgc tgtttttgat
     4141 ggtagacctg ttacttgata ggtggaagga aagacttgcg gcaattgctc tattcgaatg
     4201 ttcaagtagt caagcacttc ttctgcccat tggaattcat ggatatcaaa atatccagtt
     4261 cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca atctataaaa gatatagtct
     4321 ttgataccga caaaataagc agtttttttg aataattcag gttggctgtt ttgcagccac
     4381 ttgattttgt tcaatggtgt cattggatga atcggcgtcc cagtctgccg ataaaattct
     4441 ttgccaagca cggtatattt cagtttttct gcttcgtccg ctgctcggtt atctgcccaa
     4501 gtgatcattc gagataacgg tgttttctct tcatccaaca atatcaggcc atgcatcgca
     4561 gaggagaagg agacaaattg aatgattgtg tctgaaggca actgtttaat tacttctttt
     4621 ataacggtgc agacaccttg aaagatcaat tcctgatctt gttctgccat cccttgtgtt
     4681 tcttggatca aagggtagcg aaaataagcg gatgctgctt cttgggcttc atctgtgaaa
     4741 gcaactgctt tggtttgtgt ggttccgata tctaatccga ttgaaacgtt cattttttca
     4801 cctcaaattt tatgtatgta caaaaattta gtttaagctt gcgtatcttg gttttgaaaa
     4861 gggggagatg gcgtgttact ttattttatc attggttgct gtatcggatc atttttatgt
     4921 ttaaccgccc agcggattcc gttaggtcac tctattattt atcctagatc tcattgtgtc
     4981 aaatgctgtc gttcgctttc ttggtatgaa ttgattccga ttctctcgat catcgtccaa
     5041 cgatttcgct gccgctattg caggtgtcgg ttgcccttct actatttgtt ggcagaagcc
     5101 ttatgtggtg gtctttttgc ctggttcttt actttttctt ccgcaagaaa tttttcgacg
     5161 ttcatatgga tgctttcggc tttgctattt tcgttgatgg atttatttta cttcatggtc
     5221 cattctcaca cgctattctg ttcttgggca attttatgga ttttttggtt gcaggccggc
     5281 gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat
     5341 ggtcaatcct atctcggagc tggcgatact ctgttgatgc tttcttggag tggtggcttg
     5401 tctctagaag agttgcttca gatcttgttt cttgctagtc tgtttggaat atccgttttt
     5461 tctgtttact ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat tccttttttg
     5521 agcagtgcgt tgctgattgt ccttcatctt aggtattgaa aaaaggttgt gacaaacgac
     5581 ttgtcacaac ctctgatata tagtgttctc ttaccaaatc cttgtgactt ggagctaggc
     5641 gacaggttca caacctctcc actaaatggt gttcaatcag ctgaccctca gtattagctc
     5701 aaaatgctgg ttttacaacc tctgatatac ggtgttccat aagtagcttt ttcggcttct
     5761 tatttcttga cgccccaatg tcgacgaagg aaacctgcac gtccagaacc ttctcgataa
     5821 agattgtaat ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc tgcaggataa
     5881 aattcgcttc ttggttcgat ttttgtcacg attggcttat cgaatcgccc gctgtcttgc
     5941 aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt catcattata gtagatcact
     6001 ggacgataag aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc
     6061 tgccaataga tttccaccaa ctgatcataa gaaataactt ctggatcata ttcgatctct
     6121 actgcttctg tatgccctgt tgtgcctgtt gttacttgtt cataagttgg atttggtaca
     6181 tgcccgccag tataaccgga tgtcacagaa atgatccctg gctgctcttc aaatggtttg
     6241 accatacacc aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata aaatccccct
     6301 tttttctcat cataccttga tttccaaaag gaggataacg ttttgcttgt gacagaaaaa
     6361 acggaaacta ttatatacgt gatacggctt ctgataaatg ccaaaacgaa cgtacttgat
     6421 caaaaacggt taaatcagga atcacatatt cagcagaagg aagatttatg ttttcaccta
     6481 ttcccaatac atgcatgtta ccagccaaag ctgcttgaca accagcttct gaatcttcaa
     6541 tgaccaagca tgcatttggc ggtacattca attgctgtgc tcctttgaga aaaacttctg
     6601 gatcaggttt tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa taacttgtca
     6661 aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga
     6721 tgtttgtttt tttcaagtac tcaagaacag ctattgcacc aggcaaaacg gatgtttcat
     6781 ccattttttg aatcgcttga agatatagat tgtttttttg ctcagcaaag gctgatttct
     6841 ctttttctga gaatttctgt tccatttttc caaatgacag caatcgctct agtgactcgg
     6901 ttctggatat tcctttcagt tgctcgtttt ctttctcagt gaatggaatg gacagctggt
     6961 tagctaaaac cagccacgct tcgtaatgat aacgagctgt gtctacaaga acgccatcta
     7021 aatcgaatag tgctgctttg attttcatcg attacctcct tgaaaatgaa cgacttgaag
     7081 taaagatgcg atgtttttag ctgtgcgatg aagattatca gtggcttgct gtaaagcttc
     7141 ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc
     7201 tttttcgctt cctttaccaa cacttccgca aatagctatc acaggaacgc cctctggtac
     7261 tcttctagca atcccaactg gcgcttttcc ttttagactt tgtccatcta gacgtccttc
     7321 gcctacgatt acgaggtctg cttgctgtac ccgtttatcg aactccagta aatccaatac
     7381 gtagtcgatg ccagagacta ttttccctga agcaaatgct aacagtcctg cacccattcc
     7441 accaccagca ctactgcccg atgcctcgat tacttgaggg gcaaatctca tataaaaatc
     7501 atgcatggct tggtcagcca ctgctaattc attttctttc aagccttttt gtggtccgaa
     7561 aacaaaagaa gcgccttctt ttccgcataa aagattagta acatcagtaa tgacagtgat
     7621 tttggcttta atcagctcaa taggcacgtt cgttttatca actgtagcaa ttttacctag
     7681 attttctccc agtgcttcta ctttttgatt atcttgatcg taaaaagcat aacctagacc
     7741 ttctgccatg ccgatgccgc catcgttggt actacttccg ccaacgccga tgatgatgtg
     7801 tcgaacacct tgttgcaaca attgtcgtat cagttctcct actcctcgcg tggataacct
     7861 aagtggctgt tgttggttta gtgggacatc tttcaatccg acgatttctg ccatttcaaa
     7921 aatcgcagtt tgaccgtctg aagcgtactg gatttctttg tttttgccaa atgcattagt
     7981 aacgactgct gtttgccgct tcaaccttaa actgtctgct aatgatgaaa ccgttccttc
     8041 tcctccatcg cctataggta gcagatcata ttcagcctga acaaaaactt cttgaaaacc
     8101 tgattgaatg gcttgtgctg cctctaaggc tgtcagactt tctttgaaag aatctggcgc
     8161 aatcagaata cgcattattt tcccctccgt tccaagatca gttctttttc cagctggtat
     8221 tggtctccat tgacaaaaat aggtaatgtt tctttcgtct ctttttttat tactatttgt
     8281 ttttttgtca aaacaaatag caacctgtga tttttccaaa taaaaggaaa agaaattttt
     8341 tcccatgctg aaggaagtct tggagaaata gaaagagttc cattcgttac tgtaatacct
     8401 gaaaaaccaa atataactgc tagccatagt gctcctaatg aagcagcatg aattccgtca
     8461 tctgatgaat gcggatctgg tcctaaatca atcaggcacg cttcttggaa taaatgatac
     8521 gcccattcca tttcttggat acggcaagcc tctatggcgt gaatcgcttt actcaacgat
     8581 gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat tggttgagaa
     8641 aaatactcag gtaataagta taacagcatg acaagatctg cttgcttgct gacttggagt
     8701 tcatttactt cgctacgggc ataatcaagt aaaatagact ggctgccagg acgttctttg
     8761 taagtggtca aatcaataac tggcttactg agatacgtgt catcttgcgg aatcagctgt
     8821 tctttgttag gcactggcaa atataactta tctaaaaaat tttgacattc tttcaaaaaa
     8881 agcggatcgc cagcttgata tttaacagct aaggccacat tataatgcgc cagatagttc
     8941 gtatatgcat tattgtcgat atattctgtg tattcatctg gaccaatgac tctgtgtatc
     9001 tctaaatgat catcgacttt tacagctctg ctcagccaaa aagctgctgt ttcatgaatg
     9061 agcgtttttc cctttgtttc taaaaacgat tgatcctctg ttgcttggac atactcttga
     9121 acggcataag caaggtctgc actgatatga tgttctgcgg cagctgaagc aacttcttgt
     9181 cttgttccgg ttttgatatt caatgcagca aatttgggtg tctcttcctg accagtgtgt
     9241 gcgctttccc atgggaaaag tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg
     9301 ccttctaatc gatcataacg gtacagcagc aaattctttg ctacttcagg aaactgatat
     9361 aaaaagaaag gcaggataaa aatttccgta tcccagaaaa catgcccttt ataaccttcg
     9421 ccagttagtc ccttcgcccc tacactaaca cttggatcat tgcggggagt cataatctgt
     9481 aaatggtaag cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt
     9541 atccgatgct gatgccaaaa atctgtccaa taagacatcg actgctctaa aatttctgta
     9601 taactaacgc ttttctgggc ttgggcaata cttttcttaa acgggtctgt ttgctgaaga
     9661 gaggtagaga gcacgatgat cttctccagc gtaaaggacg tattttttct gatcttttct
     9721 gaaaaggaag cggtgatccg gcgatttttt gcttggaata cacctgcttt attgaattta
     9781 gctgcaatcc caatatcaat tttactttct gttgtctggt aggatgcact gatcaaatcg
     9841 tcaccaaata ctcgcagttc tttttcaacc aactgctgtg taccaaaatt ggtttgctgt
     9901 ccatcgatac ccgttttcac atctacttgg cagtcttcgg tcaacggctt gatgaccact
     9961 ttcagggctg cctgatggag attattttga tcagcaacac gacaagtaat gacttccact
    10021 ttttttcctt gagcagtttc aatgagcgca tagcgtttaa gctctcccat ttctaaatct
    10081 agtgcccgct cgtatgcaag aactttcatc gtctcaagag aaaaatgttc accgtcgatt
    10141 tttatttccc atctgatgac atctgggaga ttcactaact cggctgattc tgctcctaaa
    10201 ggtcggttat atacaccagc tgcaaacatt ccccttactt gattccgata gtcttcctcc
    10261 tggcttcctc tgataccaaa aaatccattt ccaatggtca tctgactcgc atacttattg
    10321 atatactcta aatcaaactg tttttcttct actttcataa ggtaataact tctcctgttt
    10381 ctgccgaacg atataaattt tccaccagat tctggatgac atatccttgc cagccatctg
    10441 caatcatctg agtctggtca ccgagcgccc gattcacaaa atgagccata cttagttcat
    10501 gtgggtcatt aactggcgtg gtcttttgta aggaaaccag ctttccatct acatcttcga
    10561 atagttcgcc tggatacaga ttagcacctg cctgatcgcc acaaaaagcc acattcagtt
    10621 tgtcttctct gatattcaaa gcaaacgaag tattcagttg gatgatcttg ccgttttcta
    10681 attccagtgt gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc catttcccga
    10741 atttccctga attttttttc gtaccaattt tttggaagct gtacgcccga acgcttttta
    10801 ctttaggaaa tccagtaata tacatcgctg tatcaagcat gtgaatgcca taatcgatca
    10861 aaggcccgcc tccttgaagt tctttgtcga taaacgttcc ccagcctgga acaccagacc
    10921 gacgaagcgc attcgcttct acgtaataaa tatccccgag aacatcaaga tgattcttga
    10981 tcattgttgc ttcgtcagaa aaacgatgct ggaatccata agccaaaatc tgcccgtttt
    11041 tatcggctgt ctctgccatc tttcttgctt gatcagctgt catagctggt ggtttctcgc
    11101 acatcactga agcattagct tcgagggcag ccaatacatg ttcgaagtga aagcgattgg
    11161 gtgtacagac actcacgata tcgggagaaa tctcattcag catttgtgta actgatgtat
    11221 acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg actggatcaa
    11281 ccacagcggc caattctaag acatctgatc ttttttgata ataagcagca tgtacttttt
    11341 ctgctacttg tccagcgcct atgattgcta ctttcttcat ttatttctct ctcctttgct
    11401 tatttactta ttgttattct ttctctattc tttctctaaa tcatttaaat gacgcgctgt
    11461 ttccagacag tttttcaagt attgcaatga ttcctgataa attttcactt cattttctcc
    11521 tcgcaaacga cattcatata ccatataacc ttcatactca ttttgaagta gcgtttgaaa
    11581 atggcgagta aaatcaattg cgccagatcc tggttggtag cgatgattat ctgctaaatg
    11641 gatatgaccc acatactctt tttgcgtagt caatgctttt gaaatatcat cttcttcaat
    11701 attcatatga taaaaatcag cgatgatctg cacatgtttc aattgattag agtcaatgta
    11761 atgacgcgca tccgctaaca aattgatcat atggtcttgg tatcggttca aaggctccaa
    11821 ataaataacc gtacctgttt cttcggctac tttatctaaa taacgaagtg attcgctgac
    11881 tgctttatag tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa
    11941 cattccccaa gctgctggga taacgattcc tttgcctcca acctcgctta gtgcacgcaa
    12001 aatctcagca atttgacgta caccattaag acgccgttct tcgataaagt caccgatcca
    12061 cccatcgtag ccgttacaag ctgtcactac tgggatccct gtttctttga ttgcctgttt
    12121 gactgtgtcg atattttcta ttaacaactt accgtcgatt tcgtattctt caaatccaca
    12181 gtctttgata aatctaaatt tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt
    12241 accgaatttc attgttctcc tcctcggact tgaacgccca ttttgatact ttcttctggg
    12301 tgctggtcta cataacgcat gtaagcttct gcgctttctg caaatggcac aactggatca
    12361 atgatttctt cacaatccaa gtaaccgttc atcaataatt cccaacatgt gtcctcgatt
    12421 cgtttacggt tccatcttgg gtaatctggg tttggttcac tagatgctcg agcaaagacg
    12481 atttttgcat tattgaaatg tgcttcgcga ccaagattaa acccagctgg aaaaggttta
    12541 gcaaaggcaa cataagaaat gatcccgcca tagccgatcc ctattaaaga agcttgtaat
    12601 gcggcagcac tgccactcgt ttcaataact gaatctactc cacgtttatc tgtcaattcc
    12661 tttagacgaa aaccgacatc tgctttcata ggatctagcg tgtaatcggc accgtgtttt
    12721 tctgctaatt ctctacgctg cgctatggga tcgatcccaa cgatggtagt agcacctgct
    12781 tttttagcta attgaatcgc aatttgcccg atagcgccta aaccgaagat tgctacagca
    12841 tcacctgctc gaacatgtgc ttcccggata ccacctaaag cgaattgagc aggatcgtaa
    12901 catactgcat ttttccattg atccaaccga tcaagtttac gtagtttata attatcaaca
    12961 ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc agactaactc tcctttttga
    13021 tactctgtca ccaaactgcc cgtttcttcg atcgtaccga caatcatatt tcctaattga
    13081 aattcgccaa ataccacacc gcgttggctt ccttcttctc ttggcaagaa taggcgccat
    13141 tcttctgaaa attcttcatc gataaatgga cttaatcctc taaagtcgac aatttctgtt
    13201 cctggttttg gagtagcgaa aattactcgg atacgtactt cgttcttttc gatctcacgg
    13261 tcttcgtaat ctttgaatcc ggctattctt ggttctaatg caactaattt tttcatcttt
    13321 tctttcctct ttcttttctt tttatccttt tacgccgcct tcgactaaat cacctttaat
    13381 aaatcgttca gataacgcat acataatgat gactggtaaa gcggtcacca atgaagcggc
    13441 catcattcgt ccccaaatgt agtctggtgt actgaataat gtgttcaaac cgattggtaa
    13501 agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat aaataatcat tccacgaaac
    13561 cataaagcaa taaacgaaga cagaaatgat tccagaaata gccaatggta taatgatccg
    13621 aataatgatc tggatccggc taagaccatc gatcattgct gcttcttcaa tatcagtcgg
    13681 aatcgtgtca aaataacttt ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat
    13741 cgtgataatc agtgctgtat tggtatcata caaacctaga ccggaaatga ttttaaataa
    13801 tggaacaacg atcaaaatac cggaaaacat ataaacggtg taaaagctag cattgatttt
    13861 cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc caatcactac
    13921 tgcagttacg gaggctatca aggagatcac taaactattt ttgaaataca tgatgaatgg
    13981 aaaaatctca gcattgaaaa tatcctgata atgttcaaaa gtccattctt tcggaaaaat
    14041 agtttgtgtc gttgcaattg cttcttgtga atccttgaat gatgtcatca gcatgatcaa
    14101 aaatggaaac aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata agattttcct
    14161 gctcttttta tttttcattt caagtccacc ttctttcgtg agagcaagac gaccgctgca
    14221 ataatgatga acaatagaat cgatatagct gctgcttttc caagatcatt gaacgcaaag
    14281 gctgtctgat acaaataaac gcctaagata ttgactttat tcgtcagtag atagacatct
    14341 gtaaacatat agaacatcca gatagatcgt aaaacaatca cagttgctag tactggtttg
    14401 attgccggca gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc catttcagct
    14461 gcttcataaa gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat aaacgcatac
    14521 ggatagtatc tccagatagc aaacaaaacg accataaaga agctagcaac tggctggtcg
    14581 aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc attgacgatt
    14641 ccataactat tattgaacat atatttccaa gcaaaaatca aagaaataga tggcgtaaca
    14701 taacttaata aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa
    14761 atagctacgg ctaatcctaa agctgtactg cctgctacaa ctaagaatgt gtaagcaact
    14821 gttaaaccta aagaacgata aaattcacta tcacttaaaa tttcaagata attgcttaat
    14881 ccgataaaaa cggcgtctga gttaggattc agcggcatat cgaaaaaact gatctgaata
    14941 ttggaaagca ttggaatcag cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt
    15001 cccatggcta attttaagtc agaatgtttc cttggacgat tcattgaaac ctccccctga
    15061 taatctgcca atagctatct acttttttcg aacaccattg gcagaacttc ttgttatttc
    15121 gtattttttt gtgcgtcagc taaagcttga tcgacatctt cgtttccgac agttacttgg
    15181 ttcactgctt ttccgatcac acctgagctt gtgatatctc ccattttcgt gaaattcttt
    15241 tccccgacta gtccaaagac ttgtacttca tcaaaagcac tagcaatctc agatggcagc
    15301 tcgccaaaag acttgatgac ttcattttct ttgtaaccat ccagttcgac tacttgttta
    15361 ttcactggtt gagcaccacc aggagacatc aagacccact tttccatgtt tttcggttga
    15421 gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag accagaagaa
    15481 atcgtcaatc cagatactgt gccatagaca gcttgatctt tttgtgttgg gatagcaaaa
    15541 ccgatatttt ctgagttccc cgcttcataa acagaaggca aaatgtaagt tgaatagatt
    15601 gccattggtg ctgttccatt catgaaagca tctttgattt ctgtcacatc attagagcct
    15661 ggcatcgtgt attgggataa gttttgataa aacgacaatg cttctctcat cttttccgta
    15721 tcgatcgtta cctcgccttc atcatccaaa acatttgctc catttgacaa agcaaattga
    15781 gagaacgctt gctcactcat tgttccttca gcagtaggca tagcaattcc atattgttta
    15841 ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt ttctggttct
    15901 gaaaaaccag cttctgataa tttttctttg tcataccaaa taccttgaac ccagccgcta
    15961 attggtgcag caatatagct ttttccatct tctgaacgaa caaggttttt ggctccgtca
    16021 taataattat cttctccagc ttcctcgata acgttttgga ctgccgtctg atcaatcagt
    16081 tcatctttat ccatcacttt agcaaaatcc tgactgactt ccattacttc tggtaattta
    16141 ccggaacgag ctaacgtaac gatttttgta ttgaacgctg attcttctac cggcacttgc
    16201 ttgattttga tgtctggatt ttctttttca aaatcagcaa ccagttcatt gatcacactt
    16261 aatcgttcct gttcgacaga agagtgcatg aattgaatcg ttgtctgttg tgattcgtta
    16321 gagctttgac tttcaccaca agcagtaaga agaaatccag tagctagcag acaggtagta
    16381 acaataattt ttttcatttt tttccacctc ttattttatt attttcagcc acatatattc
    16441 atacggctgg aatatcaact ttcgttcttg actgcaatgg ataatttgtc cagaaaacag
    16501 gtctaggtaa tctccttttg gtaaaagata acctgctgat tgattggaaa aattatggat
    16561 aactaagact ttctcttcct tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc
    16621 cagtaagtaa atcaaactat tcggatggaa ggctttttct ttttttcgaa tagatagcat
    16681 ctgggtcaat tcaccatata ctgctgttct taatggatct gtctgcaact ctttctcaat
    16741 atctgctaac aaaaattttt gccgattgat ttttcgattc tctcctgttt tttctacgcc
    16801 tttgtaatca tttcgacttc ccagcacact ttgaatataa attgccggca cgcctggtaa
    16861 cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt
    16921 tggagaactt aacgcatcta gatacgtagc gtttatctca tatggactgg tcgttccatc
    16981 tgggtttttc ttgtagttta ctcgggcacc ctctttcttc agctgttcca ctaattcaag
    17041 gatcgttttt tccggcagaa tacctcgtac cggattcaat ccgataccgt catgggaggc
    17101 taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg atggttttta accattcttt
    17161 caacttactt gtattacccg aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg
    17221 atagaccatc tgtgcttcat tctcgccatt cccaaaataa ctgatattat ctttgtgtgg
    17281 cacatttgtc tcagtgatca ataccgttcc tggagctgct tcatcagcga ttgcacggaa
    17341 taattggatg attttatgcg tttctggcag gtgtatacaa gaagttccta gttctttcca
    17401 taaaaatccc acagcgtcca agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag
    17461 aatatcgatc attttcaata aaacttctgg atttttaaaa ttcaagtcta cttgatcttc
    17521 gctaaaagtc gtccatacgt gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa
    17581 cgggctggtc cttggtctag tcacgctgct tgtatctgta tccagtggca aatcaataaa
    17641 aaaatctttg tacgtatcat tcaagtttaa atattcctga aaccaagtac tttcggcaga
    17701 catatgattg cagacaaagt cgaacattaa ccgtgtttcg cttctcattt cttctatatc
    17761 ttcccatgtg cccaaacggt cgttgattgc ctgataattt acaacggaga aaccatcatc
    17821 agatgtatat gggaaaaatg gcaagaaatg tacaactcta aatgtatctg ccaaatactt
    17881 ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc
    17941 ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt
    18001 ctgctgataa acactgactt tttgttcaat catagaggcg gttttctcag acaaatctga
    18061 gtcgcgataa atcgcttgta aaagttttgt atacatcgga ttcccctttc ttttttggga
    18121 gcgttcccaa cgatttgatc atattatgga aaacgcttac tgtcaatagc atttttttaa
    18181 ataattttga tatacacaaa aatacaaata agaaagctta tgtatcaata attaatagct
    18241 tcttaaaaaa atatggtctc tcatgatcat gcaagctatt cctaaatttt caaaatcata
    18301 gacaaaaaag aatgtctatt gtactatgga attattctaa aacgctccca aaaaggaggt
    18361 gcatataaaa ttgaaaccta ctatttatga cattgcgaaa aaagctaatg tctcaaaatc
    18421 aactgtctcc agagtattaa ataaccagcc aaatatctct gataaagcaa aagcaagtgt
    18481 acttcaagca atcaaagaat tgaattatga accgagcaaa attgcaagag gactgtcagg
    18541 aaatggtttt gacgcgattc tagcattatc tcatcgtact tctcatacga cctatggcaa
    18601 tccttttttt tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa ataacttcga
    18661 cttgattcta caaacggcaa aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat
    18721 ccaagaaaaa atgatccgag gaattattat tttaagctct acgatggatg aaacacttct
    18781 ttttgaattg gatcaatata atattcctat tgtggtaatc ggaaaagtcg gaaacgacta
    18841 cacgaacatc tactcagtgg acacggataa tttccaggat agttacgatt tggtggaaac
    18901 actgatcaag aatggacacc gtcatatcgg ctgtctccat gctccgacag aagtttatgt
    18961 atccaatgat cgtctcaatg gttaccgtca atgcttattt gatcaccacc tagatatccg
    19021 taatgaatgg attatagacg gcggctttcg cttagaagat agcatggcag caatgaatga
    19081 tttaatgaag ctacgagaaa gaccaactgc gattttttct accgatgcat taaaatcatt
    19141 gagtatttac aaagtggcag aacaatctgg aaagcagata cctgatgatc tttcgatcgc
    19201 aggattcagt gatcccttat tgtcctccct attttcgcca cagttgactc ggatagacat
    19261 tccgacaaat cagttaggaa tcaaagccac agaactttta ttccaactaa tccaccacca
    19321 ataccctgaa gaaaaaagaa tcattctttc aaccgagatt actctcacag attcgatcca
    19381 acaacaagaa atgtagaaca gaattataaa agaacagaaa cactgaggat aagtcagtta
    19441 ctctgttctt ttttcaatca ctcatccctc gttaccccct cccggtattt tagcgaattt
    19501 ttcttatttt ccatctgttt tagatagctg tggataacta taatgtttgt gttaaactat
    19561 tttttccaca gggaagaaaa aactattttt aaccacgttg tgtataagtt tttatagttc
    19621 gtttttttaa acacatttac ccttatttca atccatgtta tttggggata cttatctcta
    19681 aaacataaat agaataacag acatgtctac tggttttgtt ttattcgagg aattattcaa
    19741 taatagctag caaaatatta aaaataaccg ctctatcttc ccatcaattt attttgcatt
    19801 caaaaaatga tctatcttta cttttgttga ttgacttctc ttggaagatg atcgttccga
    19861 taccttaatt ggatacgaac cgatgttgtt aagatccttt cccacttttc ttctagttag
    19921 acaaattatt ttagacaaac caaaaaagag gttgtgacaa taatttgtca caacctcttt
    19981 tttaaaatat ctattttgtt tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa
    20041 tatctgaaat actgtagaca ttttcgcttg ctacaccgac ttcttttggt tccatgtcac
    20101 gataacgttg cataccagta ccagctggga tgatcttacc tataataaca ttttctttca
    20161 aaccaagaag aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt
    20221 cttggaatga tgcagcagat aagaaactat ttgtttctaa tgatgctttt gtgataccta
    20281 gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg tagttttgat
    20341 ctttaaagtc tgcgatgtct aataatgtac ctggtaagat atctgtatca cctggatcca
    20401 ttacacgaac tttacgcagc atttgacgaa ccattacttc gatatgcttg tcgccgattt
    20461 ctaccccttg catacggtaa acacgttgta cttcacgaag caagtagttt tcaacagata
    20521 atacatcgcg aacttgcaac aattgttttg gatcgattga tccttctgtc aatggtgcac
    20581 cacggtggat catatcgcct tctgctactt tcatacgtgc tgtataagga acagtatacg
    20641 tacgtgtatc tgtttttcct ttgattgtta cttctttggt acgtgttgca ggatcttctg
    20701 agatatcgat gacatcccct gttacttctg tgatcactgc ttgccctttc ggattacgtg
    20761 cttcaaagat ttcttggaca cgaggaagac cttgagtgat atcgtctccg gcaaccccac
    20821 ctgtatggaa tgtacgcatc gtcaactgag taccaggttc accgattgat tgagcggcga
    20881 tcgtaccaac tgcttctccg acttcaacgt cagagccagt tgcaaggttg cgtccgtaac
    20941 aatgtttgca gacaccatgt tttgtattac atgtgaagac agaacggatt gtcactgttt
    21001 cgacacctgc atcaacgatt tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa
    21061 tgatcaattc atttgtttca ggatgacgta cttctttacg ggtgtaacgt cccactaaac
    21121 gatcttctaa tgattcgatg atttcatttc cctcgcggat ggcttggatt tcaagacctc
    21181 ggtcagtacc gcaatcttct tcacggatga ttacatcttg ggccacgtca accagacgac
    21241 gagtcaagta acctgaatcg gcagtcttca aggctgtatc ggtcatccct ttacgagccc
    21301 cgtgagttga gataaacatt tccaagacgg atagtccttc acggaagttc gaaatgattg
    21361 gcaattccat gattcgacca ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg
    21421 taaagttgga aatgttacca cgagctccag aatcagacat catgaagatt ggatttttcg
    21481 cttccaaacc ttccatcagt ttttgttgga tgctgtcttt tgcagcattc caaacagcaa
    21541 tgacacgttc ataacgttcg tcatctgtga tcagaccacg acggaattgt tttgtaatag
    21601 tttcaacttg tttatgggca ttttcaatga tttcttgttt ttcattcagt acactgatgt
    21661 cagcgatccc tactgtgata ccggcatgtg tcgaatgttt gtaccctaag tctttcatac
    21721 ggtcaagcat cttagaagtt tctgttactt tgaatcgttt gaagacttct gcgatgatgt
    21781 ttcctaagtt tttcttcttg aacggtaata ctaattcttg ctctttgata tgtgcaggaa
    21841 tatcagtacc agcttcaaca aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg
    21901 ttggttcatt cagataaggg aattcaggag gcatgatctc gttgaagatg atcttaccaa
    21961 ctgttgtgat catcgtacgt tctttttgcc attcagtaaa tggtttttca cctaatgagt
    22021 tagggttcac accgatacgt gaatgtaaat gaacatatcc gttgcgccat gcgataacgg
    22081 cttcgttttg atctctgaag accattcctt cgccttcgcg tccttcttct tccattgtca
    22141 agtagtagtt acctagaacc atatcttgag aaggagtaac aactggttta ccatctttag
    22201 ggttcaagat gttttgagca gctaacatca acatacgtgc ttctgcttgt gcttcttcat
    22261 tcaatggaac gtggacagcc atttggtctc catcgaaatc ggcattataa gcttcacata
    22321 caagtggatg caaacggatt gcgcgtcctt gtactaaaac tggttcgaat gcttggatac
    22381 caagtcggtg aagcgtaggt gcccggttca gcaatactgg gtgttccttg attacgtctt
    22441 ctaaaacatc ccatacttcg tcttcttgac gttcgatttt acgtttcgca tttttgatgt
    22501 ttgaagctag ttcgcgttga acaagctcac gcatcacgaa tggtttgaat aattcaatgg
    22561 ccatttcttt tggaagtcca cattggtaca tcttcaagaa aggtccaaca acgataacgg
    22621 aacgacctga gtagtcgaca cgtttaccta gcaagttttg acggaaacga ccttgtttcc
    22681 ctttcaacat atgagaaaga gatttcaatg ggcggttacc tggtccagta actggacgtc
    22741 cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg cagcatacgt ttttcatttt
    22801 gaacgatgat tccaggtgca ttcaagtcta acaaacgttt caaacggtta ttacggttga
    22861 ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa
    22921 ccattgggcg taaatctggt ggaataacag ggataacgtc cataaccatc cagcttggtt
    22981 gatttcctga agcgcggaat gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt
    23041 tttgtccttg tgcagatttc aattcttctt tcaattcggc tgcttctgca tctagatcaa
    23101 cattatcaag taattgttta atcgcttctg cacccatagc agcttggaat tcttgtccat
    23161 attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg
    23221 tatttcctgg ttcgatgact acatatgaag caaaataaat gatttcttcc aaagcgcgtg
    23281 ggctcatgtc taatacaaga cccatacgtg aaggaatccc tttaaagtac cagatatgtg
    23341 aaacaggagc tgctaattca atatgtccca tgcgttcacg gcgaactttc gaacgagtaa
    23401 cttccactcc gcatcgatca caaacgattc ctttataacg gatacgtttg tattttccgc
    23461 aggcacattc ccagtctttt gtaggaccaa aaatccgctc acagaacaac ccttcacgtt
    23521 caggttttaa ggtacgatag ttaatcgtct caggtttttt tacttcaccg taagaccagc
    23581 ttctgatttt ttcgggagaa gccaaaccta tttgcatact ttcgaattta tttacatcga
    23641 tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc aagacggctg atcttcgacc
    23701 gccttgcagc tcatagaccg gattagtcta aattatcttc tgcggtttca aagttttgga
    23761 tgacatcgtc tttttcatct tccacttgtt cagcagcttt cttttctaat tctttggcag
    23821 tttgctgttc ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt
    23881 ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa gacacgcatg tccagaccta
    23941 gtgattgcaa ttctttcacc aatacgcgga atgattccgg tacacctggt tttggaattg
    24001 gttcaccctt gacgattgct tcgtatgttt tcacacgtcc gactacgtca tctgatttgt
    24061 atgtcaagat ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc catacttcca
    24121 tttcaccaaa acgttgtcca ccaaattgag ctttacctcc caatggttgt tgtgtaacca
    24181 atgagtaagg tccgattgaa cgtgcatgca atttgtcatc gaccatgtga gcaagtttga
    24241 tcatgtacat cacaccgaca gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt
    24301 acagaacagt tttcgcatcg ctggccatac cggcttcacg aactgtttcc catacgtctt
    24361 catcacttgc accatcaaat actggcgttg ctacgtggat acctaactga cgtgcagcca
    24421 tacccaagtg taattccaat acttgcccga tgttcatacg tgaaggcacc cctaaaggat
    24481 tcagcatgat atcgattggt gttccatcag gtaggaatgg catatcttct tccggcataa
    24541 tacgggatac aaccccttta tttccgtgac gaccggccat tttatcccct tcatggattt
    24601 tacgtttttg aacgatataa acacgaacta acatgtttac gcctggtgac aattcatctc
    24661 cagcttcacg agtaaagatt ttcacatcgt gaacgatacc gccgccgcca tgtggtacac
    24721 gtaatgatgt atcgcggact tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt
    24781 cttctgcaga taattctgtt accccttttg gcgtgacttt ccctacaagc aaatcgccat
    24841 cttggacttc tgcaccaata cggatgatac ccatttcgtc taaatctttt aatgcgtctt
    24901 ccccgacatt agggatttcg cgtgtgattt cttcaggtcc taattttgta tcgcgggctt
    24961 ctgattcata ttcttcaata tggatagaag tgtatacgtc atcttttacc aaacgacggc
    25021 tcatgatgat cgcatcttca tagttgtacc cttcccaagt catgaaacca actaaaacgt
    25081 tttggcctag agccatttcg ccttgttcca tagatggtcc gtctgccaga gtatcccctt
    25141 tttcgacttt ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt ccggagtttg
    25201 aacgacggaa ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga
    25261 tttcagaagc atcaacgtat tcaacgacac cgtcatgttt acataacaaa gcagctcctg
    25321 agtcatgcgc tgatttgtat tccataccag taccaaccca tggtgcttga ggattgatca
    25381 acggcactgc ttgacgctgc atgttggcac ccatcaaggc acggttggag tcatcgtttt
    25441 ccaagaaagg aatacatgct gtcgcaacgg caactacttg tttaggtgat acgtccatgt
    25501 agtctacttt atcaatagat acttctaagt tttcactttg agcacgagcc ataacaacgt
    25561 cttgagcaaa tgttccatct tcattcaatg ggctgtttgc ttgggctacg atgtaatggt
    25621 cttcgatatc agctgtcaag tagtcgattt ggtcagtaac acgacctgtt tcacggtcta
    25681 cacgacgata aggtgtttcg ataaatccga atttatttac tttcgcataa ctggacaaac
    25741 tattgatcaa cccgatgttt gggccttcag gagtttcgat cggacacata cggccatagt
    25801 gggagtagtg aacgtcacga acttcataac cggcgcggtc acgagtcaat ccgccaggtc
    25861 ctaaggctga aagacgacgt ttgtgtgtca attcgccaag agggtttgtt tgatccatga
    25921 actgtgacaa ctgagaagaa ccaaagaatt ctttgatact tgccacaact ggacggatat
    25981 tgatcaattg ttgaggtgtc aatgtttcag tatcttggat cgacattctt tcacgaacca
    26041 cacgttccat acgtgctagc ccgatacgga attggttttg taaaagttca ccgactgaac
    26101 ggatacgacg atttcctaag tggtcgatat catcgacatt gccgatacct tccatcaagt
    26161 tcaagaaata gctcattgaa gcaatgatgt cggctggacg aaccgttttt acagctgctt
    26221 ctggatagcc gtttccgatc acgtttactt cacgttcagg gtctcttggt gaaaatactt
    26281 tgatgacttg gacagtcatt ggatctgtga ccacaccgtc ttctgatgga tagtaagtca
    26341 ctgaatttag gccattatca atgaaaggag ctagtgtttc catgacttgg tgagtcagaa
    26401 ctgttccttt ttcaacgatg atctcgcctg tttctggatc aactaatgtt tcagctaatg
    26461 tcagattcaa tagacgtgtt ttcaaatcta attttttgtt tactttgtaa cgaccaacgt
    26521 ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt
    26581 ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg
    26641 ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc
    26701 tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa tcctaacgca cgaactaata
    26761 cggttaaagg aatcttacgt gtacggtcga tacgtacata agagatgtct tttgcatcag
    26821 tttccatttc tagccaagct ccacggttag ggataacagt agaaccgaat ccttcttttc
    26881 cgtttttgtc gacttttcca tggaaataga cacctggtga acggactaac tgagaaacga
    26941 ttactcgctc tgccccgttg atgatgaatg ttccttgttc tgtcattaat gggaaatcac
    27001 cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta
    27061 catgtaatgg tgcagagtag tttgcatcat gcgcacgagc ttctgctact gtatactttg
    27121 gttctttcaa ttcataatct acaaattcca aagatagatt gccattgaag tcatcgattg
    27181 gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa gaaccattgg taagagtctg
    27241 tttgaatttc gatcaaattt ggtaattcca acacttcact gattcttgcg aaacttctac
    27301 gttcgcgatg ttttccgtat tttactacgt gtccagccaa gctcttcacc cctctaaaat
    27361 gtcattcaaa aagactaccc gatcaattgt tacaataata tttcaaatat tatttttagg
    27421 taacaacaga cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag aacattctct
    27481 tgaaacctta cttcgtgagc ttctactttt gtttccttat attaaagccg aaacggacaa
    27541 tatttcgttt ctgccttttc ttcatcag
//
LOCUS       DAZUMB010000027        26290 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000027 DAZUMB010000000
VERSION     DAZUMB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.027"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..530)
                     /locus_tag="ACG80L_000952"
     CDS             complement(162..530)
                     /locus_tag="ACG80L_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS5023228.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            680..1162
                     /locus_tag="ACG80L_000953"
     CDS             680..1162
                     /locus_tag="ACG80L_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS5023229.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1199..2209
                     /locus_tag="ACG80L_000954"
     CDS             1199..2209
                     /locus_tag="ACG80L_000954"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS5023230.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2324..3037)
                     /locus_tag="ACG80L_000955"
     CDS             complement(2324..3037)
                     /locus_tag="ACG80L_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023231.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3210..3817)
                     /locus_tag="ACG80L_000956"
                     /pseudo
     CDS             complement(3210..3817)
                     /locus_tag="ACG80L_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4301..4528
                     /locus_tag="ACG80L_000957"
     CDS             4301..4528
                     /locus_tag="ACG80L_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS5023232.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4544..5710
                     /gene="hemW"
                     /locus_tag="ACG80L_000958"
     CDS             4544..5710
                     /gene="hemW"
                     /locus_tag="ACG80L_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS5023233.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5944..6987
                     /gene="hrcA"
                     /locus_tag="ACG80L_000959"
     CDS             5944..6987
                     /gene="hrcA"
                     /locus_tag="ACG80L_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS5023234.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7081..7644
                     /gene="grpE"
                     /locus_tag="ACG80L_000960"
     CDS             7081..7644
                     /gene="grpE"
                     /locus_tag="ACG80L_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS5023235.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7695..9524
                     /gene="dnaK"
                     /locus_tag="ACG80L_000961"
     CDS             7695..9524
                     /gene="dnaK"
                     /locus_tag="ACG80L_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS5023236.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9675..10841
                     /gene="dnaJ"
                     /locus_tag="ACG80L_000962"
     CDS             9675..10841
                     /gene="dnaJ"
                     /locus_tag="ACG80L_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS5023237.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10923..11552)
                     /locus_tag="ACG80L_000963"
     CDS             complement(10923..11552)
                     /locus_tag="ACG80L_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5023238.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11564..11737
                     /locus_tag="ACG80L_000964"
     CDS             11564..11737
                     /locus_tag="ACG80L_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023239.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11782..12519)
                     /locus_tag="ACG80L_000965"
     CDS             complement(11782..12519)
                     /locus_tag="ACG80L_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5023240.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12521..14023)
                     /locus_tag="ACG80L_000966"
     CDS             complement(12521..14023)
                     /locus_tag="ACG80L_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS5023241.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14282..16276
                     /gene="uvrB"
                     /locus_tag="ACG80L_000967"
     CDS             14282..16276
                     /gene="uvrB"
                     /locus_tag="ACG80L_000967"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS5023242.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16286..19102
                     /gene="uvrA"
                     /locus_tag="ACG80L_000968"
     CDS             16286..19102
                     /gene="uvrA"
                     /locus_tag="ACG80L_000968"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS5023243.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19365..20630
                     /locus_tag="ACG80L_000969"
     CDS             19365..20630
                     /locus_tag="ACG80L_000969"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS5023244.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20634..21365
                     /locus_tag="ACG80L_000970"
     CDS             20634..21365
                     /locus_tag="ACG80L_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS5023245.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21500..22384
                     /gene="rapZ"
                     /locus_tag="ACG80L_000971"
     CDS             21500..22384
                     /gene="rapZ"
                     /locus_tag="ACG80L_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS5023246.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22381..23379
                     /gene="yvcK"
                     /locus_tag="ACG80L_000972"
     CDS             22381..23379
                     /gene="yvcK"
                     /locus_tag="ACG80L_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS5023247.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23413..24348
                     /gene="whiA"
                     /locus_tag="ACG80L_000973"
     CDS             23413..24348
                     /gene="whiA"
                     /locus_tag="ACG80L_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS5023248.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24891..25541
                     /locus_tag="ACG80L_000974"
     CDS             24891..25541
                     /locus_tag="ACG80L_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023249.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25728..>26290
                     /locus_tag="ACG80L_000975"
     CDS             25728..>26290
                     /locus_tag="ACG80L_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFS5023250.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8678 a         4439 c         5592 g         7581 t
ORIGIN      
        1 cagtgatttt tttcagcaca agaaagactc cttataaaaa tagcagatac tttgtcaatt
       61 aagtgtttaa ccagggatga taaaaaggat aggattgaac aaaacctgta taaggtgatg
      121 aatagtttcc tcgccagtaa aaggtttatt cgtataaagc tttatcgcca aaatttatca
      181 tgaccttttt tatcttcaat attaaatgaa atcaattgtt caatcaattc ccagttgatt
      241 acacttttcc aaggaacttt gatgacgtta tccgtatata cgtagcctgc ttcttggata
      301 tttttttcaa gggcacgaat cgctgctttt tccggtgcga tagaaaaatg atttttagca
      361 aaactaaatg caataataaa ggtaccatgg tcagtaaaca taggttggtt ccattttaca
      421 gttgtgtcta gctggggata cgtttctgtt atcttagcaa aaaggtcttt gatcttttgt
      481 tgatgttctt tttctttgat ttgattgatg atcggctgaa atttttccat tctaaaacgc
      541 ctccattaag tggtttgatt tattttacca gaaaaacaat agttatgtat aaaaaacgaa
      601 caaaacacaa gtaatgttgt aaccgctata tttatatgat aagatgtctc aaataataaa
      661 atagaaaaga gacataaaaa tgattgactt ggcaaagaaa ttcttacacg aaaatatata
      721 ttttgagttg tatcatcatc gagccattta tacaaatgaa gatgctttgg taataaagaa
      781 agaacaagga tttacaggta cagagaccaa aagcttattc ttaaaagata aaaaagataa
      841 taactacgta tatctgactt ttactacgaa acaaacggat ttcaaagcat tagggaagat
      901 cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg gaagaacaaa cgggacaaaa
      961 gcagggcgct gtttctccgt ttggttatga aaaacagatt ccgattatta ttgatgaaga
     1021 gctcttatca aatgaaaaac ttgtttttgc accaggtaga ccagatcaaa cgatggtcat
     1081 ccaaacggtt gacttaaaca aaatcatcca gctacttgag atgccgatat ttttgatgcc
     1141 ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat acttggaggt gaagcaacat
     1201 gataagttat ccattattag aaagaaatgc aacgattggg gttactgcac catcctcagg
     1261 cgtatcagag gaacaccatt cgttactcga atcagcttta aagagaatgg aagaaaaatc
     1321 tttccaagta gttactggaa agacgctttg gacgcaagat aaggaaaaat caggtccagc
     1381 aagaaaacga gcagatgaat tcaatcaaat gatgcaagat gaaaatatcc aaatgatctt
     1441 tccgacttgg ggaggagaat tattgattga gattctcgat tttgtcgatt ttgatgctct
     1501 acaaccaaaa tggatagtag gttattcaga tattagtacg ttgctacttg ctgtcacatt
     1561 aaaaacaggg atagccactg cacatggaac taaccttatt gatttaagag gagagtactt
     1621 ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca agagaaggag aatcgattct
     1681 acagcaatct tctgaaaagt atcaaaaagt gtggccccat gagcattcat catcttctgt
     1741 tttccatctc actgaaccaa caaattggca atcagtcagc ggagatgcag taaggatgaa
     1801 gggacgatta ttaggtggtt gtattgatac gatcaggcat ttaatcggta ctccttatgg
     1861 agatgtccaa tcttttaaaa gaaagtataa tgatggaaag ccgatcttgt ggtttctaga
     1921 aaactgtgag ctatcgtcag ttgatttaag aagatcactt gttcagatga aactggcagg
     1981 ctggtttgat ggcagctcgg ggatcatgtt cggtagaagt gcggcagagg atttgttaga
     2041 ttataaaatg aaggatgtat acgaagatct gtcaaaggaa ttaaaactac cgatcttgta
     2101 tgatattgat tgtggtcatg taccgccaca actgacattg attaatggtg cctatgcaga
     2161 agtagcggta gaagatagaa aagcgactgt tttgcaaacg tttatttaaa aaattttttc
     2221 ctgccttgga gtctatcaaa gaccaataaa tctaaataga aaagaaagtg aaagaagctg
     2281 acaaaatgct gtcagcttct ttttgtataa catattagaa agtctaatta tctataactg
     2341 tcacatcgat taactgtttc acgtcattgg gatttcctgt aggaatagct actacagatg
     2401 cattatcaat gatttcttga gttgtttctc taatgaacgc tggataagat gtttgatctg
     2461 caggcatttg ccaatgtgaa ggaactggat aactagtgat tgtcccgtca tggttacttg
     2521 aatacactaa aactccctgg taactgtatt tcccagtgag cataaccaca tctttcggat
     2581 agactgcact ccctgcatca taaggggcga taggtgttcc tgcaggataa tgaataacat
     2641 gtaattcgaa atcacccgat gtatctctaa aagtattacc tctaatagct agccaaacac
     2701 gtgcgtactc aatttgtaga tcagaatatc ctgctaacgg atctattgac tgagaagact
     2761 gtgaggaggt tgtgtctatt gttgaagaag aaatatttgt ctcaactgta ctactagatg
     2821 tagaagaagt attgggctct gagactttac tgcttgttga agatgtattc gtttcagtag
     2881 aagctgattt ctgagtactt gatttttcag ctgaactggt ttgtctgatt gaacttttgt
     2941 ctgtggtcgc agatggatca ctattttctt tactaggaga cgtacatcct acaaggaata
     3001 aagagaccaa caataaaata aaacctaact ttttcatata aaatacctca ttattttttt
     3061 aataccagta tagcataatc ctgaattttt gaatccgtaa aaccttgtct atgaatataa
     3121 atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataatc atcagtaaaa
     3181 ttacttgtca cactattgat aaggagtatt taattgaaga gaatgattag gagaaaggat
     3241 ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc gttttttctc
     3301 ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat tctataggag
     3361 ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct ataaaatctt
     3421 ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg gtgtgtttgt
     3481 aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata gcagctactg
     3541 gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt tttatgtccc
     3601 atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt tcgattaagt
     3661 aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata gaagcagttt
     3721 tgttttgcca tttctcattt tggtttagtg tgctagtaat ataagcacct tcccaatcgg
     3781 catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc tttactatct
     3841 ataacaagga caacagtttt aacctacaga cattcattaa aattgggtag cgacagcgaa
     3901 aacaacaatt cttaagtttt agacagagta tacagatagt caacttaatt aaatacgaac
     3961 taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt tgcatacatg
     4021 gatgacaaaa gaaccaattc ccacttgctt gagtacaata ttaaaagaaa ctggaaggca
     4081 aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg aaaacaaccc
     4141 tagagatata taactaggta accagtttaa tgaaagaaaa aatcatccaa gaagtcgatt
     4201 cttggatttt ttagtggatt tgttcgccaa atacgtttag tatgcgtata tgagaggttg
     4261 taagcaaagc gttgtggtct gagcacttaa ggaggaatct gtgaaaatag cttttcatat
     4321 ttttgcagat tttgacttaa tgccgaaggt cagctttgcg attaccgttt atcgagaggc
     4381 tgtaaaagca ccgtgtagct ccgatgacaa taagcaagca tttttagagc taggtttcct
     4441 tgattctaaa tatgcggctt atttcaatga gttggtgctt gaacgcgatt ttaagaaggt
     4501 cgaaaacagg cacgactttt cgacgtaagg agaaagaatt tcaatgatag aaacaataaa
     4561 tcccgcagaa aacaaggttt ctgcgtacat tcatatccca ttttgcgagc atatctgcta
     4621 ttactgtgat tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat
     4681 gctgctgaat gaaatgaaaa taatgctaga aagtcatccg acagcgaatt tagaaacact
     4741 ttacgtaggg ggcgggacac caacttcttt atctgctaaa caacttgatc gactgttatc
     4801 aggtgctaga gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc
     4861 aggtgatttg acccgtgaaa aacttcaagt aatgaaaaac tatggcgtca atcgtttatc
     4921 catgggagtg cagacatttg ataatcgctt attgaaaaaa attggacgta aacacaccgc
     4981 agaagatgtg tatcaaacta tgcaattttt agaagcagaa aattttacca atgtaagcat
     5041 cgatttgatt tatgcattac cgggacaaac actagaagga tatctggata ctttggaaaa
     5101 agctttggct ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac
     5161 aatgttcatg aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac
     5221 cagaatgttt gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc aatatgaaat
     5281 aagtaacttt gggctctccg gacatgaatc tcaacataac ctcatgtact ggaacaatga
     5341 ccactatttt gggtttggag ctggtgctag cggttatctt ggagatattc gttaccgcaa
     5401 caaaggacca atccaacact atttacgccc attacgagca aatgaacgcc cggtattgga
     5461 agaagaaaaa ttgacacgaa aaaatcaaat agaagaagaa atgttcttag gactaagaaa
     5521 aaaaacaggg attactaaaa aacatttcta tgatcgctat caaaaaacaa ttgaatcggt
     5581 gtacggtgat gtgctaaggg aattagagca agaagggtta cttgttaatg aagaagaccg
     5641 tgtttatttg acaccaagag ggaccttttt ggggaacaat gtgtttgagc gctttttgtt
     5701 tgaaaaatag tttaaagaat atttgttttt tatctgaatt taaataaaaa aagttcaaaa
     5761 atacaataaa ggactattga aacgttgata taactggatt tacatcaatt atttagaatt
     5821 agcactcttg tgtttagagt gctaaaaaat ggagtctttc tcttgacagg agaagggctt
     5881 cttgctatat ttataagtgg attagcactt agaatatatg agtgctaatg agaggtgagg
     5941 aagatgttaa caaagcgaca aaacgacatt ttacgtctga tcatccaaca ctatacgagt
     6001 agtggcgtgc ccgttggttc caaaacattg atgtctgagg gagtcaaggc cagctcagca
     6061 acgattcgca atgatatgaa agcattagaa gatgaaggat tgctgttgaa gactcactct
     6121 tcttctgggc ggattccatc tgctttaggc tatcgttatt acgtggatca tttgttaaaa
     6181 cctgctcgcg tagcagaaga tgatttgctg ctgattcgtc agtctctagg cagagaattt
     6241 catgaaatca atgagattat taagcaatct gctgagatat tatctgagct gacaagctat
     6301 acgacttttt cattgggacc tgaaataaaa gaccgccgac tgactggctt tcgtatcgta
     6361 cccttgaata atcgacagat tattgctatc gtcgtaacag atatggggaa tgtagaaagc
     6421 caagtgttca ctcttcccga atatatggga agtcaggaac tagaaaaaat ggtacagatt
     6481 gtaaacgacc gtctagtcgg agatcctctg atgacggtgt accataaact tcgtacggag
     6541 attccgatga ttcttcataa atatttccaa acgacagaag ggatgtctca gttatttgac
     6601 accgttcttg gtcatgtttt tgaagataag gtttatgtca gtggacaaat gaatctcttg
     6661 gattatgagc cccgtcaaga tcttgatcag ttcaaatcga tgttttcttt tatgaaagac
     6721 tcagacggac ttacacaaat gatcactccg atggatagca atattcatat caagattggt
     6781 gctgaattag gcaatgattt attgcagaat atgagtatga ttcaagcaag ttacgagata
     6841 gatggtcatg gaagaggtac aatagccttg ttagggccta ctagtatgcc ttattctcgg
     6901 atgttcggtc tagtcgatgt ttatcgaaga gaactagcag ctaaacttgc tgattactac
     6961 cgttcgcttg acttgtcaaa ctcgtaatgt gaataacgaa gctatttgca gctgatcgtt
     7021 ctctctttct tagtaagagc aatcaaacag ctgactgaat aatgaaagga agactgtatc
     7081 gtgaaagaaa atcaagaaga actagataaa gaaatgacag atgctcagcc agaaccggaa
     7141 atcgatgtcg aagctgcaga agattcgggt atttcagaag ctgaagcgga agaattcgaa
     7201 acagcgaaat taaaagctga gttagaagaa atggaagata aatacttgcg ggctcgtgct
     7261 gaaatcgcaa atatggcaaa tcgaggaaaa aatgagcgtg agcaattaca aaaataccgt
     7321 tcacaagatt tagccaaaaa attacttcca tcaattgaca acctggaacg tgcattagcc
     7381 acagaagtgt cagatgatca aggggcaggt ttgaaaaaag gcgtggaaat ggtcttggaa
     7441 agtttgcgta acgcactgga agaagaagga attgaaaaaa tccctgctaa aggagaagcc
     7501 tttgatccta atcttcatca agcagtacaa actgttccag cgactgaaga tacaccagcc
     7561 gatacgattg tagaagtact acaagaaggc tacaaattac atgatcgcgt gttaagacca
     7621 accatggtca ttgttgcaca ataattgtgc ggatataaaa cttacacttt caattaaaga
     7681 aggagacgtt aattatgagt aaaattatcg gtattgactt aggtacgact aactctgcag
     7741 tagcagtatt ggaaggaaac gaagcaaaaa tcatcgctaa cccagaagga aaccgtacaa
     7801 caccttctgt tgtatcattc aaaaatggag aaatccaagt aggggaagtt gcaaaacgtc
     7861 aagctgtgac aaacccaaat acgatttcat caatcaaacg ccatatcggt gaagcaggtt
     7921 acaaagtaga tgtagaaggt aaatcatata caccacaaga aatctcagca atgatccttc
     7981 aatatatcaa aggattcgca gaagactatt taggtgaaaa agtcgaaaaa gcagttatca
     8041 ctgtgccagc ttactttaat gatgcacaac gtcaagcaac aaaagatgct ggtaagatcg
     8101 ctggtctaga agtagaacgt atcgtcaatg aaccaactgc agctgctctt gcctatggtt
     8161 tagataaaac agatcgcgac gaaaaaatct tagtattcga ccttggtggc ggtacttttg
     8221 acgtatctat ccttgaacta ggagacggtg tctttgatgt attgtcaaca gctggcgaca
     8281 accatctagg tggggacgac tttgataaca aaatcatcga ctacatggta gcagaattca
     8341 aaaaagaaaa cagcattgac ttgtctcaag acaaaatggc tttacaacgc ttgaaagacg
     8401 ctgctgaaaa agcgaaaaaa gatttatctg gcgtatcaag cacacaaatc agcttgccat
     8461 ttatcacagc aggagaatct ggaccattgc atttggaaat gacattgaca cgtgcaaaat
     8521 ttgatgaatt aacagctgac ttagtagaac gtacaaaagt accagtacgt caagcgctaa
     8581 aagatgcagg tttatcacaa tcagaaatcg acgaagttat tttagtcggt ggatctactc
     8641 gtatccctgc agttgtagaa gcagtacgta aagaaacagg aaaagaacca aacaaatcag
     8701 ttaaccctga tgaagtagtt gctatgggtg ctgctatcca aggtggcgtg atcactggtg
     8761 atgtgaaaga tgttgtttta cttgacgtta caccattgtc attaggtatc gaaacaatgg
     8821 gtggagtgtt cactaaattg attgaccgca atacaacgat tccaacaagt aaatcacaag
     8881 tattctcaac tgcggcagat aatcaaccag cagtagacat ccatgtatta caaggtgaac
     8941 gcccaatggc tgctgacaac aaaactctag gaagattcca attgacagat attccagcag
     9001 ctcctcgtgg tattccacaa atcgaagtaa cattcgatat tgataaaaac gggattgtaa
     9061 atgtatctgc gaaagatcta ggtacacaaa aagaacaaaa aatcacgatc aaatcttctt
     9121 caggtctaac agacgaagaa atcgaacgta tggtaaaaga tgcagaagca aatgcagaag
     9181 cagataaagc tcgtaaagag gaagttgacc tacgtaatga tgtagatgca ttgctattct
     9241 ctgtggacaa aacattgaaa gaattagaag gcaaagtgga cgaagaagaa gtgaaaaaag
     9301 cagaaacagc tcgcgatgaa ttgaaagccg ctgttgaagc aaacaacatc gaagaaatga
     9361 aaactaaacg cgatgcattg aacgaaatcg ttcaaaactt gactgttaaa ctatacgaac
     9421 aagcggcaca acaacaagct caagaaaacc cagaagcagc tcaaggcgga gcagatgatg
     9481 tcgttgatgc tgattttgaa gaagtcgatg gcgacgataa ataatcagtt cactgaattt
     9541 cgagagaagg ccaaagttgc tactttggct ttttcttttg aaaaatagtt tgaagtatcg
     9601 gtttttgttt caaacaaaat atgatatgat aacaagcgac gtgaaaatga tgagaaccaa
     9661 taggaggaaa gcctatggca acaaaacgtg attattatga agtcttaggg ctctcaaaag
     9721 gagcatcaga agatgaaata aagaaagctt atcgtaagct ttcgaaaaaa taccatcctg
     9781 acatcaataa agaacctgat gcggaagaaa aattcaaaga agtatcagaa gcatatgaga
     9841 ttttaagtga cccgcaaaaa cgcgctgcat atgaccaata cgggcatgca ggcacagatc
     9901 caaattacgg tgctggtgga gctggtggcg gatttggtgg attcggcggc ttctctggtg
     9961 gcggcttcgg tggttttgaa gatatcttcg actctttctt tggaggaggc ggtcggtcag
    10021 tcgatccaaa tgcgcctcgt caaggtgctg atttgcaata tacggtcgac ttgtcttttg
    10081 aagaagcgat ttttggtgta gaaaaagaaa tcaagtataa tcgggaagaa atctgtcata
    10141 cttgtggtgg gaacggtgca aaaccaggca cacaaccaac tacttgtcat aaatgtcatg
    10201 gtgcaggtac gattaatgta gaacgccaaa caccacttgg acgagtgatg agccgtcaaa
    10261 cttgtgatgt ttgtcatggg acagggaaag aaatcaaaga accttgtcca acctgtcatg
    10321 gctcaggaca tgagaagaaa gcacattcag ttaaagtgaa tgtaccagct ggtgtagaag
    10381 aaggacaaca aatgcgttta gctggtcaag gagaagctgg agaaaacggc ggaccatttg
    10441 gtgatttgta tgtggtcttc cgtgtagaag aaagtgatat ctttgatcgt gaaggttctg
    10501 aaatttacta tgaattgcct ttaagttttg tacaagcagc tttgggggat gaagtccaag
    10561 taccaactgt ccatggagat gtgaaattga agatccctgc tggaacacag acaggtacga
    10621 acttccgttt acgtggaaaa ggcgctccga agttacgtgg tggtgcaaca ggcgaccaac
    10681 atgtcaaagt aaaactgatc acaccgaaaa atctaaatga acaacaaaga gaagcattac
    10741 gtgcattcgc tgaagctggc ggcatcaaag ttgaagaaca gcaagaagac ggtttcttcg
    10801 ataagtttaa agatgctttt ggcgggaaaa agcggaagta gtttttctag taaaaacgct
    10861 aagtcattaa taattaaaaa ccagacgaac aatcatatgt tcgcctggtt tttttgtagt
    10921 tgtcatgttt gtaccaacaa aaaagccagc tttagaatat gataatcgtc atagtttcta
    10981 ggggtataac cagtgagaga atggaacttg ttcaatcgat attgcaaggt gtttttatga
    11041 ataaataatt cttctgagca atttttgatg cttccgttat ttttactgaa gcatgccaaa
    11101 gtcttcttga acaaatgtac ttcttcttct gtcaggcttt tcaaaatacg ttttacatat
    11161 aatgttcgtt gatctgacgg gattgctggt aaaacgatcc cgaggtctaa atcttcaaaa
    11221 aagcggatct cgttcacaga ttgatggaaa gctatccatt cgcaaacttg tttggcatga
    11281 aggaaagatt gcggtgtctc ttgctctgta taacaaagcg acccaattcc aaagtttagc
    11341 tgtaactgat acattttatt aaactttgtc tggattttat caagcaatga tagcaatgag
    11401 gctctggaaa tccgattgac taggataatg atttgttgtc tatgtacagt gagcaaagcc
    11461 tgtccgtaat aagagaagac cctttcgagc aactccaata aaggattgat ccgttcttga
    11521 cacaattgct gattgtcaga aagttgtccc acaactacat aatatggaga agaaaaatca
    11581 agctcaatta agtgagggtg gtatcgacta tgtacttgat atcatcaatg aaccgatggt
    11641 actttcttat gcgatggaac aggtagagcg attgcttgtt tgtgcaggaa aacaagcgat
    11701 gcggctgata cgattaggtg agaaaataag aagataaaaa tacacggtcc cttcatggcg
    11761 accgtgcatt tttggttttt attaaatatt taatacttta ttcaagaaat cttttgtccg
    11821 ttcattttgc ggattttcaa agatttgttg aggcgttcca tcttctaaaa agtttccgtc
    11881 gtcaatgaac atgacgcgat tggccacttc tttagcgaac cccatttcgt gcgtaacgat
    11941 aaccatcgtc ataccttgtt ctgctaactt tttcatgacg ttcaagacat caccgaccat
    12001 ttcaggatcg agtgcagaag taggctcatc aaaaagcatg atgtcagggt tcatcgctag
    12061 agcacgagca atagccacac gctgtttttg tccaccagaa agcatttcag gatacatatc
    12121 ttttttatct tctagaccaa ctgtctgcag taatttgatt gcttttttct ctgcttcttc
    12181 tctagattct ttcttcaggt ccatcggtgc taaaatgatg ttttccaaaa tagaaagatg
    12241 agggaatagg ttaaagtgtt ggaataccat tccgatatgt tgtctcacca agttgatatc
    12301 tgtgctttta tccatcaagt gggcgccgtc aataataatt tcaccgtcag aaggttcttc
    12361 taaacgattc aagcaacgca agaatgtact ttttcctgaa cctgaagggc caatgataca
    12421 gacgacatct ccttcgttga tggatacatt gatgtcgttt aatactgtgt tatcgccgta
    12481 ttttttgact aaatgctcaa ctaagatttt ttctgccatc ttacttcacc ttcttttcta
    12541 aaacttttgc taattttgtc aatgctgtga tcaaaatcaa gtacatgatc gcaatgatca
    12601 agtaaacata cgtactttgt gttgtacgtg caacaatgat cttaccagtt tgcagcaatt
    12661 cgactacacc gattgcggaa atgatcgttg tgtcttttaa tgagataacg aactgattga
    12721 cgaatgatgg gatcatgatg cgtatcgctt gcggcaaaat gattttttgc attgtccgat
    12781 tataagaaag acctaaactt ctggaagctt ccatttgtcc gacaggaacg gcgttgatcc
    12841 cgcctcgaac aatctctgcg atataagcac ttgcgttaag agtcaaggtg ataacaccag
    12901 caaggaagtc aggaatcgta aatccaacga taccaggcaa cccaaagaaa ataaagaacg
    12961 caagaaccat cattggaatt cctcggatga tatcgacata aatactagca attgtgcgaa
    13021 gagcgcgtat tggtgctaca ctgaataatc caaaaatcac accgattact atagccaaag
    13081 caaaggagat caaagttagg agaattgttc tccaaagtcc tttcaacaat gcttgatagt
    13141 tgttttggat aagtccaaga aatgttgatt catcagcagt ggaagactca ctggaagttt
    13201 ctgaaccatc cgctacatat tctttaataa tcttatcgta ttcgcctgtt ctttttaatt
    13261 ctttcaatcc ttcattgaac atattaagaa gctcaggatt ttgtcctttt ttaacggcaa
    13321 atgcatattt tccgccagat tcttttttga tcggtgtttc caattcttgt ccttgtttga
    13381 tcgcataacc gattaccgga taatcatcca tcattgcatc gattgcgcca acttttaacc
    13441 cgtcgtacaa ttgatcagca gcatcaaaat atttgataga atagccatat tcagattcat
    13501 ggtcgcttaa aaattcagca ctttcggtac caacttttgc tccgactgtt tttcctttca
    13561 aatcttcata agacttgatc gaagtatctc cttttttgac agctaattgg atcccgctat
    13621 caaagtaaga atcagaaaaa tcatagcttt tctttcgttc atctgttaca gtcattccgg
    13681 caatcattcc gtctgcttgt cctgattcca aagcttgcaa agcagcagag aaaccacggt
    13741 agtccattcg gatgttaaat ccttgcagtt tggcgatgtt ttccatcaat tttacatcga
    13801 tgccttcgta ctcgcccttt gcgttttgaa attcaaatgg agcaaaagca gagtcactgg
    13861 caattacgta tacgtctttt ttgggctcta ttttttgcat tgtctcgctt gtgcttgact
    13921 ctgccgttgt agtgttgtca gcttgtgcaa catgaccaaa cgcaaatgtc acaagtcctg
    13981 tcattaagaa aacaaatgag tgaaataaat gctttctttt cataatcaac ctcctaaatt
    14041 tccaatattt tattattata ggcgaaaagt gctgaatcag acaagactgt aagctcgaaa
    14101 aaatgaaaaa aatgcgagtt tctaacattt tatgttataa aacataacat gtataataag
    14161 ataaaaaagt ggcgaaagta tgttcggtgt ggtataattt tttagtaagg aatgcctttg
    14221 ctttcctttt ctttttattg gtcgtaaaat gaaaaggaat gaatgaaaag gaggacgaag
    14281 aatgattgag agagatacct cccgacactt tgagcttgtc tcgaaatacc aaccggctgg
    14341 tgatcagcct gaagcgatta accaattagt ggatggcgta gtcggcggga aaaaagcaca
    14401 aatcttatta ggtgcgactg gaacagggaa aacatatacg atttccaatc ttattgagaa
    14461 agtgaataaa ccaacactga ttattgccca taacaaaaca ttggctggtc agctgtacgg
    14521 ggaattcaag gaatttttcc ctaacaatgc tgttgaatat tttgtttctt attatgatta
    14581 ttatcagcct gaagcttatg tgccttcaag cgatacgtat atcgaaaaag attcaagtgt
    14641 aaatgatgag atcgacaaat tacgacattc ggctactagt tcattattag aaagaaatga
    14701 tgtgatcgtc attgcatccg tttcatgtat ttttggatta ggctcgcctt ttgagtacca
    14761 aaaacaagta gtttcgattc gacagggagc agaactagat cgcaatcagt tgattcgtga
    14821 cttagtcagt atccaattcg aaagaaatga tatagatttt cagcgcggtc gttttcgagt
    14881 gcgcggtgat gtcgtggaga tttttccggc ttcaagagat gaacgtgcct tacgtgtgga
    14941 gttttttgga gatgaggtcg aacgtatccg agaagtcaat gcactgacag gtgaagtttt
    15001 aggagaaacg gaacacgtag caatcttccc ggcgactcac tttgtgacga atgatgaaca
    15061 tatggagcat gccgtagcga atatcaaagc ggaattagaa cagcgtttaa ctgtcttgcg
    15121 aaatgaaaac aaacttctgg aagctcaacg tttagaacag cgaaccaatt acgatattga
    15181 aatgatgtta gaaatgggct acacgtcggg aattgaaaat tattctcggc atatggacgg
    15241 acgtaaagaa ggtgaaccgc cttatacgct attggatttc tttcctgaag attttttgat
    15301 cgtggcggac gaatcgcacg taaccatgcc tcaaatcaga ggaatgtata atggtgaccg
    15361 agcgagaaaa cagatgctag tagattacgg tttccgtttg ccatctgctt tagacaatcg
    15421 gccacttcgg ttagaagagt ttgaaaaaca tgtcaatcag atcatttatg tctctgcgac
    15481 gccaggacca tatgaacatg aacaaacaga taccgttatc caacaaatca tccgtccgac
    15541 agggctgctt gacccagtga tcgaagttcg tccaatcatg ggacaaatcg atgatctagt
    15601 aggcgaaata aatgaacgag tcgaaaaaga ccagcgggtg tttgtaacga cattgacgaa
    15661 aaaaatggca gaagacctca cagattattt caaagaatta gggatcaaag tcaaatattt
    15721 gcatagtgat atcaaaacgc tggaacgaac agaaatcatt cgagatcttc gattaggtga
    15781 atttgatgtg ttagttggaa tcaatttgct tcgtgaagga ttagacgtac ctgaagtttc
    15841 ccttgttgct atcttggatg cggataaaga aggcttttta cgtagtgaac gttccttagt
    15901 ccaaacgatt ggacgagctg ctcggaatga agaaggtaaa gtaatcatgt acgcagacaa
    15961 agtaacagat tctatgcgtc tagcgatgga tgaaacgtca agacgtcgta cgattcagca
    16021 gaagtacaat gaagaacatg ggatcgttcc aaaaacgatc attaaagaaa tccgagattt
    16081 gatttctatt acgaaagaat cggaagatga tacaaaagaa gcggtacaag tctcgtacga
    16141 agaaatgacg aaggaagaaa aagacacgtt attgatgaaa ttagaaaaag aaatgaaaga
    16201 tgcggcaaaa gctttggatt ttgaaacagc cgcgaatgtg cgagatatga ttttagaatt
    16261 aaaagcttca aagtaggagg acaacatggc taacgataaa attacgattc atggtgcacg
    16321 tgcccataac ttgaaaaata tagatgtaac gattccacga gataaattcg tggtcgttac
    16381 aggtttgtcc ggttctggaa aaagttccct agcatttgat acgctgtacg ctgaagggca
    16441 gcggagatac gtagagagcc tttctgctta tgctcgtcag tttttaggac aaatggataa
    16501 gccggacgta gatagcatcg acggattgag tccggcgatt tctattgatc agaaaacaac
    16561 aagtaaaaat cctcggtcga cagtaggtac tgtgacggaa atcaatgact atttacgctt
    16621 gttgtttgca cgtgtaggac atccgatttg tcccaacgat catatcgaga ttaccagtca
    16681 atcagtagat caaatggcag atcaagtaat ggctttgcct gagcgcacaa agatccaagt
    16741 gttggcaccg atcgtggtaa agaaaaaagg acaacataaa aaaatattcg aacgaatcca
    16801 aaaagaggga tacgtccgcg ttcgagtcga tggtgagatc tatgatttaa gtgaagtgcc
    16861 tgagctggag aaaaacaaaa aacatgatat cgcgatcgtc atcgaccgga tcattgtgaa
    16921 agaagggatt cgttctcgcc tatttgattc tttagaggcg gccctgcgat tagcagacgg
    16981 ctatgctctg gttgacgtga tcggtgaaga agaaatgttg tttagcgaac actatgcctg
    17041 tccttattgc ggatttactg ttggcgaact agaaccaaga ctgttctcat tcaatgcgcc
    17101 ttttggagct tgtccagact gtgatggttt aggtgtcaaa ttagaagtcg atcaagattt
    17161 agtgattcca gatagaacga agacacttag tcaaggtgct atcgttccat ggaatccaat
    17221 cagctctcag tattatcctc aaatgttagc gcaggcttgt gaaagtttcg gtatcgattt
    17281 agatacacca ttcgaagaat taccggaaga gcatcaggag atcatcttga atgggacaga
    17341 tgatcttttc catttccatt atgaaaatga ttttggtggc gttcgcgatg tagaaacgac
    17401 tttcgaaggg gtattgaaga atatcgagcg tcgttatcat gatacgaaca gtgatttcac
    17461 gcgtgagcaa atgcgtcttt atatgacaga attaacttgt cagacatgta aaggttatcg
    17521 attgaatccc caagctctat ctgttcaaat caatggaacg aatatcgggc aaacaaatga
    17581 gttgtctatt caaaaagcag tagatttctt ctcaaactta acgttatctg aacaagagaa
    17641 agtaattgcc aaaccgattt taaaagaagt aaatgacaga ctttcattct tggaaaatgt
    17701 gggactagac tatctaactt tgagtcgcgc ttccggaaca ttatctgggg gagaagcgca
    17761 gcgaatcaga ttggctacac aaattggatc gaatctttct ggagttttgt atattttaga
    17821 tgaaccatcg atcggattgc atcaacgcga caacgatcga ctgattggct cattaaagaa
    17881 aatgcgtgat cttggcaaca ccctcgttgt agtcgaacat gatgaagata cgatgtgggc
    17941 agcagattat ttgattgacg tcggacctgg ggccggagaa caaggcggac aaatcgtggc
    18001 tgcaggcact cctgaagaag tagctaatga tgaaaattct ttgacaggga aatatctttc
    18061 agggaaaaaa tcaatccctg taccaaaaga acgccgtaaa ggtaatggga aagcaatcaa
    18121 gatcactggt gcttcagaaa acaatctgaa aaatattagt gttgaatttc cattaggaga
    18181 atttgtagca gtcacaggtg tgtctggttc tggaaagagt acattggtca atagtatctt
    18241 aaagaaatcg ttagcgcaaa aattaaataa gaattctgct aagccaggta aattcaagac
    18301 aatttccggc tacgaaagta tcgaaaagat catcgatatc gatcaaagcc caatcggccg
    18361 gacgccgaga agtaatccag cgacttatac aagtgtattt gatgatatcc gtgggttatt
    18421 tgctcaaacg aacgaggcaa aaatgcgggg ttataagaaa gggcgtttta gtttcaacgt
    18481 aaaaggcggt cgttgtgaag cttgtcgcgg ggatggaatt attaagatcg aaatgcactt
    18541 tttgcctgat gtctatgttc cttgtgaagt atgtcatggt aaacgatata attctgaaac
    18601 attagaagtg cattacaaag gaaaaagcat tgctgatatt ttggaaatga cagtagagga
    18661 tgctgtagaa ttcttcaagc acattccaaa gattcatcgc aaactgcaaa cgattgttga
    18721 tgttggctta ggttatgtga cgatggggca accagcaacg acattgtccg gtggtgaggc
    18781 acaacggatg aaacttgcca gtgaattgca caaaatctct aatggaaaga atttctatat
    18841 actagatgaa ccaacgacag gacttcatag cgatgacatc gcccgcttgt tgcatgtatt
    18901 acaaagatta gtagatgctg gtaacacagt tttagtgatt gaacacaatc tagatgtaat
    18961 caaaacagca gattatatca ttgatttagg accagaaggt ggagaaggtg gaggaacgat
    19021 ccttgcgact ggaacaccag aagaaatcat taacgtaaaa gaaagttata caggtcacta
    19081 tttgaaaaaa ataatggtat aaagaggata gatagctgag atagttaaaa aaacataaaa
    19141 aaatgaattt ttgcttgttt tattaatatc agtgttctag aatcattaca tggtagttct
    19201 ttctacactg attggtatag ataaaaactt cctataagtt caatccgttc aaaaaagaat
    19261 tgagcaatcg gaggactttc ctttgattgc ttttttcttt atggtatgat attccttgtg
    19321 tagttggaaa agaaaaacaa gagattatga gtaagtgaga gataatgatg aacagtattg
    19381 aaaatgaaga atttatccct atattattag gaagcgacat gaacgtttat ggtatggcac
    19441 gttcttttaa tgaagcatac ggcaaaatct gccaagcata tgcttcggat cagttagccc
    19501 ctacccgcta cagtaaaatt gtcaatgtag aagttattcc aggatttgat aaagaccctg
    19561 tatttatcga gacgatgctt cgtttggcaa aagaaagata ttctgataag agtaaaaaat
    19621 atcttttgat tgcttgtggt gatggatatg cagaattgat ctcccaacat aaacaagagc
    19681 tgtcagaata ttttatctgt ccatatattg attattcttt atttgaaagg ctgatcaata
    19741 aagtaagctt ttatgaagtt tgtgaagaat atgatcttcc gtatcctaaa acattgatcg
    19801 tcagagaaga aatgttagta aatggacatt tggaacagga attgcctttt gaattcccag
    19861 ttgctttgaa acctgcaaac agtgtggagt atctttctgt ccaatttgaa ggaagaaaaa
    19921 aagcttttat tttagagact agagaagagt tcgatttaat tttaggccgg atatacgaag
    19981 ccggttataa aagtgaaatg attgtacaag attttatccc gggtgatgat agtaatatgc
    20041 gtgtgctaaa tgcgtatgta gatgaagatc atcaagttcg gatgatgtgc cttggtcatc
    20101 cactattaga agatcctaca ccagcatcga ttggtaatta cgttgtgatc atgccagatt
    20161 ataacgaaaa aatctatcaa accattaagg catttttgga aaaaatcgaa tatacaggct
    20221 ttgccaattt cgatatgaag tatgatccta gagatggaga atataaatta tttgaaatca
    20281 atttgagaca aggtcgtagc agtttctttg tgacattgaa cggcttgaat ctggctcgtt
    20341 ttgtcacaga agatcgagtg ttcaacaaac cctttgttga aacgacctat ggaacgaatc
    20401 aatcagataa agcaagatta tggatgggtg tgcccaaaaa aatcttttta gaatatgctc
    20461 gggaaaatga agacaaaaaa ctagctgagc aaatgatcaa agaaaatcgt tacggtacta
    20521 ctgtatttta tgaaaaagac cgatcaatca agcgctggtt gttgatgaaa tatatgttcc
    20581 ataactatat tccaagattc aaaaagtatt ttcatgtgaa agaaggctag ttcatggaga
    20641 attttttcag tattttaggc ggaatgggca cgatggcgac agaaagtttt gttcgcttga
    20701 tcaaccaccg aacgaaagct acaaaagatc aagaatattt gaattatgtc ttattcaatc
    20761 atgcaacagt tcctgatcgt acagcttata ttttagatcg atcagaagaa aatccaatgc
    20821 cgtttctgct ggatgatatt gagaaacaaa atttattgcg accaaacttc attgtgttga
    20881 cttgtaacac cgcacactac ttttttgaag agttacaagc ggcaacggat attcctatct
    20941 tgcatatgcc aagagaagca gcaaatgaac ttgtccgtca gcatacgaca gggagagtag
    21001 cgattctagg aacagaagga agtatgaaag ctggaatcta tgaacgcgaa gtcaaaaatc
    21061 ttggtttcga aacagtgatt cctgatactg cgcttcaaga aaaaatcaat tacctgatct
    21121 atcatgagat caaggaatcg gattatttga atcaagagct ctattacgaa atcttagagg
    21181 aagcggtaga gcgcctaaac tgtgaaaaag tcatcctagg ttgcacagaa ttatctttga
    21241 tgcatgaatt tgctgaagat aatcattacc cagtgatcga tgctcaatct attttagcgg
    21301 atcgaacgat cgaacgtgca ttagctgaaa gaagcgaggc attggataca gcatcggaaa
    21361 agtaaaaaaa ttggatttgg ggcaaatatc ctaaaagata tttggtccaa atctttttgt
    21421 aaaagaaaaa gaaaactggt aatgatacgc ccgggatgat ataattttat tatgcacaat
    21481 gtgagagggg ttcgtaaata tgacagataa tttgcaatta gtcatcataa caggtatgag
    21541 tggtgcaggg aaaacagtag ctatccaaag ttttgaagat atgggctatt tttgtatcga
    21601 taatatgccg cctcgattga ttccaaaatt ctgggaattg atcaaagaat caggtaaagt
    21661 cacaaaaatg gctttaggag tggatttgcg ttctcgttcc ttctttgaag aaatccaaaa
    21721 catgctgatc gaaatcgaaa atacgaactt catcgacact tctatcttat ttttagatgc
    21781 ttcagacgaa gaactagtgg ctcgctacaa agaaacaaga cgagcacatc caatggcgat
    21841 ggacggtttg atcactgaag ggatacgtaa agaacgggcg atactagacg acttgaaagc
    21901 ccaagcttcc gtgatcattg atacgacaac attgacacca cgacaattac gtgaaaaaat
    21961 caatgagtca ttcaaatcaa agggagacca aggatttcgg atcgaatttg tatcatttgg
    22021 ttttaaatac gggttgccga tcgattcgga tattgtgatg gatgtgcgtt ttttgcccaa
    22081 ccctcattat atcccggaac taagaccatt gaccggaaaa gatcagccag tctatgatta
    22141 tgtgatgtcc tttgatgaaa cgcaatcatt ctaccataaa tttttggatc ttctggaaac
    22201 tgtattgcct ggatacatta aagaaggaaa aagcagcttg acagttgcaa tcggctgtac
    22261 aggcggtcaa caccgttctg tggcattgac tgaacgtttg gcaaatgctt tagggaaaaa
    22321 ctataaagtc aatgtgacac atcgggacaa ggataaaagg aaagagacgg tgaaccggtc
    22381 gtgaaaatga aaacctaccg gataagaaaa ccaaagatcg tagtggtcgg cggtggcact
    22441 ggtctgccag ttatcctgaa aagtttacga aatcaaggag cagatatcac agcagtagtg
    22501 actgttgcag atgatggtgg aagcagcggt gcaatcagag aatcgatcgc tatggctcct
    22561 cctggtgact tgagaaatgt cttagtggcg ttatcggata tgccacaatt ctatgaagat
    22621 attttccaat accgtttcaa aaaggaagat cagtttttag ccaatcatac gatcggtaat
    22681 ctgatcattg ccgcagtttc cgagatgcgg ggaagcactt atgaggcgat tcaattatta
    22741 gcaaaaatga tgcatgtaga tggacatgtt tatccttctt cagaaactcc tttgaccttg
    22801 catgctgttt ttaaagacgg aacgactgca gtaggagaat ctaaaatcgc gatcaaccgt
    22861 aaaaccattg accgtgtgta tgtctgcaac acaaatgata aaagtgaagc caaagcggct
    22921 cgaaaagtcg tttcagcaat tatggacgcc gacatggtcg ttttgggacc cggaagttta
    22981 ttcaccagta tccttccaaa tttagtgata ccagaaatcg gcgaagcaat gctcaagaca
    23041 actgctgaaa ccgtctatat ctgtaatatc atgacccaaa aaggggaaac ggaacatttc
    23101 acagatgccg atcatatacg tgttttacac aagcatttga atcagccttt tatcgataca
    23161 gtattggtca atacggaaaa agtgcctgaa gattatatgg acccagagat ctatgatgag
    23221 tatctagtac aagtcgcaca cgacttcaac ggattgagaa atgaaggatg tcgagtgatt
    23281 tccaccgact ttttagaatt gaaagatggt ggtgtcttcc acgatggaga caaagtagta
    23341 gaagaattgt tccgtttagt tttcggttca aaatattaag caatttattg ttggagaaag
    23401 gaggagaata ggatgtcatt tgctgcagat gtgaagaaag aactaactgg tttggaagtt
    23461 catcgagaac atgcgaaagc ggaattagct gctttgctgc gaatgaacgg ctctttaggt
    23521 attgtgaacc agcagtttat tttgaacgtc caaacagaaa atgctgcaat tgccagacgt
    23581 atctattctc ttttgaaaga tcattatcag gtacgaagtg agctactcgt tcgtaaaaaa
    23641 atgaaattaa aaaagaacaa tgtctatatc gtccggttga agcaaggcac aaaagatgtg
    23701 ttgatggatc ttgatatcat ggatggtatg atgttcaatg gacacgtttc ggacgagatt
    23761 atgggaaatt cgcaaaaaat gcgttcttac ttacgtggtg cttttatggc ctcaggttca
    23821 gtcaataatc cggaaacgag tcgttatcat ttggaaatat tttctattta tgaacagcat
    23881 aaccaagaca tttgcgatat gttaaattat tacggtctac atgctcgaac gcttgaacgg
    23941 cgcaatggct atatttcgta tttaaaagga gcagaaaaaa ttgctgattt cttaacattg
    24001 attggtgcaa ccaactcaat gctccgattt gaagatgttc gaatcgtacg ggatatgaga
    24061 aattcggtca atcgtctagt caattgtgag acagcaaatc tgaacaagac gattgatgcg
    24121 gcttctaaac aaatcgaaaa catcgaattg atcgaagcaa aggtcggtct acatgccctt
    24181 cctgaaaaac ttcaggaaat cgcagaactt cggcttcaac atccagaggt cagcttgaaa
    24241 gaattagggg aaatgattcc ctcaggtgca atatcaaaat cgggaattaa tcaccgaatt
    24301 cgcaaaataa atgaatttgc ggaaaaactg cgtgaagaag cagtttaatc caaagaaaaa
    24361 actgatacat gaagcaggag atgcgaatgt atcagttttt tttaaaagct atacttgctt
    24421 ttcttccgtc aaactataat tccaacttta ctggagtaac ataatgttga aaggcttcta
    24481 ttgtttttta atacaatcgt ataaaaagcg gggagacaac tgaatgaaga ttttagtttt
    24541 atacataaac ttttgcttaa tatagaagtc ctgatataga taggatcaag tatttatcta
    24601 taactggaga ttaattaaaa actggaagga actgctcgat caaccttgag aactgttttt
    24661 cttacttctg gtcaagattc tcgtggttgg tggataagga tggttgataa gttcattgga
    24721 ttagctagtc gaaaacaacc aggtgtaatt aatgtagaag ttttaacaag gggatggagt
    24781 gatttattca gtgataatca taagttcata aatagtaatt gggatgtatg gttggtatac
    24841 gttatacctg aacttgaact atcaagattt ggtttagaat ttgagaatcg atgtttataa
    24901 agggagggca aaaaatgtgc ttttttacaa cttttactat cattatgatt gcagtacttt
    24961 ttatttttct agatatagca aagcgaaata cagcattttt attatatcgg gttttacttc
    25021 gagcaggtct cataacattt atatcgatcg ttggattttt tctattcaca gttattgtat
    25081 ttatttggag aactacagca ccaccattgc cagagattac atatggagaa tttccgttta
    25141 gtcttgaata tgaaatagat gaggaaagaa ttttgattga agatacggtg attgcagaat
    25201 ttataggaag ttttagaggg aatgtgacaa ctcaacctag acgaagttgg agtataaaac
    25261 ttttaaataa tagtggagag ttgaaagcat ttcattatga attcttaata atagaaagtg
    25321 aagatgtaac aatcacttta agtagtggct ctgctggata ttttatgaat gatggtggaa
    25381 gcaagccaca gctcacagtc cgtgatcgta ttgcaggttt tatgcatttt tctcactatg
    25441 acttagaaga agctcgtaaa atacttgcag aatatgggat cacattaatc aattgggaat
    25501 atgcgccacc gataagaaat tttttcaatg attattatta gtcttataaa aaacagaatg
    25561 taaaatttat gtgcgcgagt atttagtttt ccattttttg tttgatagat atatagcatt
    25621 gttgaagcat gggatagtgg tgccaatttt atcaatgagg ttggtcaggc agttaacata
    25681 attggtgatg ctataaatcc atttaattgg aattggagga attaggtgtg gaaacagtga
    25741 gattatggat attcatgaaa aattttcgtt cggctttgag atatgttatt ctggaatcaa
    25801 aatcaggtta ttatctgatt gacacagatc actatacttt ttggggaggc tacctttttc
    25861 cgatttttaa ttggataatc ccccataagg ttatgaaaat taacttgcct gcagaagaaa
    25921 taaatagatt aatactcaac aaagatttac aaaggaaaag agagaaagaa cattctaatg
    25981 ttgcttggat aggtccagca tttgcagtag tgacttcttc aattgtcggt cctatgctag
    26041 agagtattta tagtgatttt tctattggaa tacgttattt atttgttact ttaagtatag
    26101 ctgttctttt gtgcataaag ggttatgtta gcaagaagaa taaagatatt ttaacactaa
    26161 tcggtgtaaa caatttatct agtactaaaa ttatactgtt tcctgaatct ataaaagaag
    26221 gatcaaggtt gttgataatt tttataatgc ttatactgtt ttctgtttta tttggggtga
    26281 tgtttataga
//
LOCUS       DAZUMB010000028        25656 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000028 DAZUMB010000000
VERSION     DAZUMB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.028"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1295)
                     /locus_tag="ACG80L_000976"
     CDS             complement(<1..1295)
                     /locus_tag="ACG80L_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5023251.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQL"
     gene            1500..2801
                     /locus_tag="ACG80L_000977"
     CDS             1500..2801
                     /locus_tag="ACG80L_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5023252.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            2804..3655
                     /locus_tag="ACG80L_000978"
     CDS             2804..3655
                     /locus_tag="ACG80L_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5023253.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            3668..4483
                     /locus_tag="ACG80L_000979"
     CDS             3668..4483
                     /locus_tag="ACG80L_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS5023254.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            4634..5800
                     /locus_tag="ACG80L_000980"
     CDS             4634..5800
                     /locus_tag="ACG80L_000980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5023255.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            6030..6677
                     /locus_tag="ACG80L_000981"
     CDS             6030..6677
                     /locus_tag="ACG80L_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5023256.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            6761..7705
                     /locus_tag="ACG80L_000982"
     CDS             6761..7705
                     /locus_tag="ACG80L_000982"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS5023257.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            7840..9291
                     /locus_tag="ACG80L_000983"
     CDS             7840..9291
                     /locus_tag="ACG80L_000983"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS5023258.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            9312..10148
                     /locus_tag="ACG80L_000984"
     CDS             9312..10148
                     /locus_tag="ACG80L_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5023259.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            10148..10807
                     /locus_tag="ACG80L_000985"
     CDS             10148..10807
                     /locus_tag="ACG80L_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5023260.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            10933..11622
                     /locus_tag="ACG80L_000986"
     CDS             10933..11622
                     /locus_tag="ACG80L_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023261.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            11628..12584
                     /locus_tag="ACG80L_000987"
     CDS             11628..12584
                     /locus_tag="ACG80L_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS5023262.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            12628..13140
                     /locus_tag="ACG80L_000988"
     CDS             12628..13140
                     /locus_tag="ACG80L_000988"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5023263.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(13396..13944)
                     /locus_tag="ACG80L_000989"
     CDS             complement(13396..13944)
                     /locus_tag="ACG80L_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5023264.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(14033..15214)
                     /locus_tag="ACG80L_000990"
     CDS             complement(14033..15214)
                     /locus_tag="ACG80L_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS5023265.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(15450..16958)
                     /locus_tag="ACG80L_000991"
     CDS             complement(15450..16958)
                     /locus_tag="ACG80L_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS5023266.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            17117..17308
                     /locus_tag="ACG80L_000992"
     CDS             17117..17308
                     /locus_tag="ACG80L_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023267.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            17511..18428
                     /locus_tag="ACG80L_000993"
     CDS             17511..18428
                     /locus_tag="ACG80L_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS5023268.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            18421..19494
                     /locus_tag="ACG80L_000994"
     CDS             18421..19494
                     /locus_tag="ACG80L_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023269.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            19642..20160
                     /locus_tag="ACG80L_000995"
     CDS             19642..20160
                     /locus_tag="ACG80L_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023270.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            20141..20533
                     /locus_tag="ACG80L_000996"
     CDS             20141..20533
                     /locus_tag="ACG80L_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS5023271.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            20609..21544
                     /locus_tag="ACG80L_000997"
     CDS             20609..21544
                     /locus_tag="ACG80L_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5023272.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            21677..22456
                     /locus_tag="ACG80L_000998"
     CDS             21677..22456
                     /locus_tag="ACG80L_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5023273.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            22449..22766
                     /locus_tag="ACG80L_000999"
     CDS             22449..22766
                     /locus_tag="ACG80L_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5023274.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            23010..24620
                     /locus_tag="ACG80L_001000"
     CDS             23010..24620
                     /locus_tag="ACG80L_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5023275.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            24777..25625
                     /locus_tag="ACG80L_001001"
     CDS             24777..25625
                     /locus_tag="ACG80L_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5023276.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8125 a         4614 c         5132 g         7785 t
ORIGIN      
        1 agttgggtag tgttcttagt tttcttgaaa tattttcctc gatataaaaa gattcgttct
       61 cggtagtttt gaaagtttct aaagccatag gcaattcgtt ttagtacttt gatatggttg
      121 ttcaaacatt ctatagggcc gttggaatag ggaactcgca acgctcgttt gatttctgtt
      181 cgatatttct tgaaggtcgt gaacactggt tgatagtgct ctggcaactc cttgtagtct
      241 tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgatagcgga aagaaaaacc
      301 tggtagactt cgtagcctcg ctttaaggag tcatcataat ataatagccg atcgactatc
      361 tccgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg accattcaat
      421 tcccacgcat tcttttgaag tagtctccag tatttcttta agtgcttcga ccgacgatcc
      481 ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaggcccg accgatgtgt
      541 tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc tttcaccaat
      601 gtgcgatagg gtgtatagaa atctgaaacc actagacgta cttgttcacg gacttttcta
      661 gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac aatatcaatc
      721 agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac gtttttcacc
      781 gatttgaatt catcgaaaca caccacttct ggtaacggct ggttcaaaga aacggtttta
      841 ggccgaaggc tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt catacgtgca
      901 atcaaagaca tagaaatagg ctcacttaat agctcaagaa tggcttgttt gactctgcgg
      961 gcaatggagc agcgacgatc gctaactgaa tcttctgcta aaaaagtctt tccacagtgt
     1021 ttacatttga agcgctgttt gttaatccgc acaatggtct tgtagttact gacatcattc
     1081 aataagatgg atactttttt ccagccccat ttaatgatcg tttgcttatt ttgatagtgg
     1141 caatggaagc attcctctgg ttgataggtc aatgtgccat caaaaacaaa acatgtttcg
     1201 tcattaatgc gctctttgcg aaaggcatct tcatgaaagg taagatttag gtctagaata
     1261 tctagtaact ctttggtaaa ctctgaatag gacatcttga aacactcctt tgaatggttt
     1321 tcgtcgactt tattctaaag gatttcaagg tgtctgtttg cgtttattag aaaaaagaaa
     1381 aagtattggc aacagaaggc gtcaccaaca ctagatatta tagatccaaa ttatcttgct
     1441 cttgttgcca ttatcttaaa agcagtttgc ggtttagcaa gtttagaggg ggcattcaga
     1501 tgcagcaaga taaaacaagc aatatcaacg aaaaaaaaat cacgctttgt tcacttattc
     1561 cagggatggg gcagtttgta aatggccaag cttttaaagg tatcgttttt ttcgccatat
     1621 tcgtcctatt cattggtcag atgcttctgg gcggatttca tgcattggta ggattggtta
     1681 ctttgggaag tataccaatg gaagatcatt cattatttat tttgattgaa ggcactttgc
     1741 aattcatcat tacaatcatt ttcttgattt tttacggttt gaatctttta gacgcttatc
     1801 gagtagcgaa gatgcgtcaa gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac
     1861 ataatatagg tgatgaaggt tttccttatc tattgacact tcccgcttat cttctcatga
     1921 tttttacgat cattttccca gtcctagtta cattgttcac tgcatttact aattatgact
     1981 tcaatcatat tccaccagcc agtttaattg attgggtagg ggctaaaaac ttttttagta
     2041 tcttcttttt aagctcatat cgtaatactt tttttgcagt cttttcgtgg accgtgattt
     2101 ggactttatg tgcaacaact ttgcaaatca cccttggtat tctcacagcg gttgttgctc
     2161 accagaaatt tattaaattc aaacgtattt tcggtatcat tttcttatta ccatgggcgg
     2221 tcccagcttt tattactatc atgagttttt cgaatatatt caacgatagc gtaggtgcca
     2281 tcaatactca agtgattcct ttcataaacc agcttccttt tgtagatatc agtgcaatct
     2341 cttggaaaac ggacccgaac tggacgaaag tagcaattat cttagtgcag ggctggttag
     2401 gatttcccta tatctatgtg atggttacag gaatcttaca atcgatttca gaagacttat
     2461 atgaagcagc aaaaatcgac ggagcaaatg catggcagcg cttttcaaac atcacgcttc
     2521 ctgctgtctt cctagtggca gcgccaacat ttattacaca gtatacgggg aatttcaata
     2581 atttctccat gatctatctg tttaacaatg gcggtcccgg aagcgtaggc ggaggagctg
     2641 gttctactga tattcttatt tcctggatct ataaacttac gactggaact tctccgcagt
     2701 attctatggc ttcagcaatc acgttgatta tctccgtcat cgttatctgt gtgtcattgc
     2761 tggtatttaa aaagacaaat gcgttcaata tgggggatta acaatgaaaa aatttcattc
     2821 gagtgctcga ttccaaaaac gactttctca cttttttact tacttttttc ttttaatact
     2881 gtcagtcatt atcatctatc cgcttttgat cacagcaagt actgccttca agtcaggaaa
     2941 tattgcagct tttagtttag atctttcaag tgactggact ttaggaaact ttactcggct
     3001 attcagtgaa acactttacg ggacttggta taaaaataca ttgatcattt ctgtcctaac
     3061 aatgatcgta caagtgactg tagtaacttt ggcgggctat acttatagtc gctatcgttt
     3121 tatcggccgc aaatcaagtt tgatgttctt tttagtgatt cagatggtac cgactatggc
     3181 agcattgact gccttttatg tcatggcttt cttattagaa gcgctcgata agtactggtt
     3241 cttgacaatg atctatatag gcggtggaat tccaatgaat acttggctga tgaaaggata
     3301 ctttgacact gtaccaatag atttagatga atctgctaaa cttgatgggg cagggcattt
     3361 caggattttt ttccagattg tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt
     3421 atgggctttt atgggacctt ttggggattt catgttagct aagttcctgc ttcgaacacc
     3481 tgaaaatcag actgttgctg tcggcttaca aacatttatt tctgatgtga caaaccaaag
     3541 agtctctctt tttgctgctg gggcaatttt gattgcacta ccgatttgtc tattattctt
     3601 ctttttacaa aagaactttg ttaccggcct gacatctggc ggaacaaaag gataggagca
     3661 gagtatcatg caaacatttc cgttaaacta tttttccgca ttacgtacgc caactcaagt
     3721 gtttgcggga cgcaaactat tatcatggcc taaatttttc ttgatatttg tatttttagt
     3781 ttccttgatg gtcatgcctg tgactctttt ttatgccaat cagatccaag cgattccatt
     3841 agaacaattt ctatccgttc attcgctgat cgatgaacaa ggaacccaaa aattttctga
     3901 acttgaatta tctgaaactg ggttacaagc atcgcaacaa acgatagctg tgacccctga
     3961 aattttggta ggtgtgtctt tatctgagaa acaacaatcc gatcatggaa cttttattga
     4021 ttttgagaag gaacaatggg tgatccaaca aaaggataaa tctggtatac gccgctatac
     4081 gatgaattat agcccatctt tccaaccaga ttctgtacgt acgcccgaag atttccaacg
     4141 atttttagaa agagaatttt atgccagtaa tcgtccaaca atcattttat cttatagttt
     4201 aagtcttggt cttgtcctat ttgtcatgac gagtctcatt ttattcgggg cttccttttt
     4261 cttgtggatg acaagaaaaa gccaactttc atccattcat acattcaaag agagtgcaaa
     4321 tttgatgctg aatgttatgg gaataggttc catggcagca gcagttgtag ggtttatcca
     4381 ctttgacttt atcttgatgc taagtgtcca aacaacgata actgtcttgc tgcttttatg
     4441 ggtttttgtc aaaacaagat tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta
     4501 tgcgacaatg aatgtgaaaa aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa
     4561 attaaacttc ttagttgatt cttttctttt ttagtattaa cgtaataggg tagttttttt
     4621 aaggaggaaa atcatgttag agaaaacttt atacaatcaa ttattttcac gttctttctc
     4681 gctccctgtc gaagtaaatt attgggacgg tacaacgaaa caatacggag atacggacac
     4741 tcctccccaa atcaaaatca atatccatga agaaatccca atgaaagaga ttacaagtaa
     4801 cgcatctttg gcactaggag aagcctatat ggatcatcga atcgagatcg agggaagtat
     4861 ccaagcattg atcaacgatg cttatcagca agcaaacagc tttttacgag gaaaagatta
     4921 tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca
     4981 tgcgcattat gacttaggta acgactttta taaactttgg ctagatccaa ctttgactta
     5041 ttcttgtgct tatttcaata cacctgaaga tacgttggaa caagcacaaa tcaataaagt
     5101 ccatcatatc ttagacaaac tctttatcaa agaaggcgaa acgttgcttg atatcggttg
     5161 tggctgggga acattgatgt tcactgctac caaagagtat aatgtcaagg caactgggat
     5221 cacactaagt gaagaacaat acgatttgat taccaaaaga ataaaagaag aacatttaga
     5281 agataaatgc cgtgtattat taatggatta ccgcgaacta aaaggcgaaa cgtttgatca
     5341 tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag gttatttcaa
     5401 agtggtaaaa gatttattga aaccaaaagg aacagctttg atccatggaa tcagccgaca
     5461 acaaggcggt gcgacaaatg cttggatcaa taaatatatt ttccctggcg gttatgtccc
     5521 tggtgttact gaattagttg ataatattac aaaaaacgat ttgcaactga ttgatttgga
     5581 aagcttacgt cgtgactacc aattgacatt agaacattgg acaaagaatt ttcatcaagt
     5641 ccaagatcaa gtcacgaaag aaatgggtga ttccttctat cgaatgtggg atctatattt
     5701 acaagcttgt gcagcttcat ttgaatcaag taatatcgat gtgattcaat atttgcttgt
     5761 tcaacctgga aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt
     5821 aaagactatt taaaaagaag ctaagtttta tgatgctcca gaagtgaact tttttgtcta
     5881 acttctagga ttgtctctaa agcttagttt ctttttcttt atattttcat aataaaagtt
     5941 tggttcttta atttttaaac atgacagaaa attttttctt cgttatactt gtaatagaat
     6001 actattttat aacaaagggg atcgaatcaa tgaaaaaaac tgttatttat actagtttat
     6061 ttgcagcttt gtttttagct gcttgtcaat caaataccaa tcaaggcaca tctgggaata
     6121 cgtcttccag tagcgaacag cgctcttcac aggtatcgga ctcctcaaca aaagcaagca
     6181 catcttcgtc tagccagaca aaaagttcta gtgaagcaag tactcagtca gatatcaaag
     6241 tgtctgcagc tcgtgcgatc gaattgttcc aagataaata cccagatact gcaattacta
     6301 gtttggaatt agatagtgat tggggatctt acttctacaa gatagaaggt gtcgatgatc
     6361 aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa aatggagaca gagaatccag
     6421 aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa agaagatggt ctggatgttg
     6481 ataaattgat cacgattgag gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta
     6541 cggcaacaga ttgggatttg gataaagatt taggtactac ttattgggaa gtcaaagtaa
     6601 aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa
     6661 ctgaacaaga tgattaagaa tttcttcgga aaacaagagt tgatttttct tttagtcatg
     6721 atacactaga caaaataact tgtttgaagg agcgatttac ttgagtttag aaacgacctt
     6781 tgccaatcat atctttgcca atcctttgat gaatgcatct ggtgtccact gcatgactac
     6841 ccaggaatta gatgaactgg ctcattcaga agcaggagca tttatcacta agagctgtac
     6901 gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt gatgttcctt tagggagtat
     6961 caattctatg ggacttccaa acttaggttt ttcttattat ctagagtatg ctcttgcgta
     7021 tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt tccattgccg ggatgagtgt
     7081 ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt gggtttaatg ggatcactga
     7141 attaaattta tcttgtccca atgtgcctgg aaaaccacaa ctagcttacg attttgaggc
     7201 cacttatgaa acactgaaag aagtattcag catcttttct aagccgctag gaatcaaatt
     7261 gcctccttat tttgattttg ctcattttga tcagatggct gatatcttga atcagtttcc
     7321 attgacctat gtcaatgcta tcaacagtgt gggcaatggt ttgtatatcg atacagaaca
     7381 agaagcagta gtcatcaaac caaaagaagg gttcggcggc attggcggag agtatatcaa
     7441 gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat
     7501 cattggtaca ggtgggattc ggacaggaca agatgcattt gaacatctgc tatgtggcgc
     7561 aagcatgtta cagattggta ccgagcttca taaagaaggg ccggaaattt tttctagaat
     7621 cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat acgtcaattg acgaatttaa
     7681 aggcaaatta cgcaccattt catagcaaag agacgaaggt catgattgat cttcgtcttt
     7741 ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg tactcattgt atgttagacg
     7801 aacttgtggg aaaataacag aggcaaaaag gaggataata tgaatgatgc aacagaatta
     7861 gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac tagttacgga tatacagaaa
     7921 aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt
     7981 aattcccacg agctattgat tccccagtca gaaaaagggc cattggctgg tatcccattt
     8041 cctttaaaaa tgctcggtca agaaaaaaag ggatggctag ctacttcagg atctcgttta
     8101 tttgaaacac accgagcttc tcatacatct aattatgtgc ggcaagcaga agcgataggt
     8161 ctagttcctt ttggacaaac caatgcgcca gagtttggtt tcaaaaatat tacagaccca
     8221 gtcatctatg gcccagcaag aaacccttgg aaccttgaat gtacaccggg cggatcaagt
     8281 ggaggagctg ctgctgcagt ttcatcagga atcgttcctt tagctggtgc tagtgatggc
     8341 ggtggttcta ttagaatacc cgcttcgttt tgcggattga ttgggctgaa accttcgcga
     8401 gggacgatgc cagttggtcc atatgcttgg agaggatggc aaggagctgc tattgatttc
     8461 ggcttgacag tttctatgag agacacggag gctttatttt atgggatgag aagtatccat
     8521 tccggtgcgc catatcaagt atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg
     8581 ttgaagatag ctttttgtgt tgattcaccg atccactcac agattagtga agaggcagta
     8641 cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag ggcatgattt gacagagatt
     8701 ccttacccac tagatggtcg taaactgatc acgtcttatt atcagatgaa tggcgcagaa
     8761 acagcagcaa tgatccgctc gattgaaaca ggcatccagc gagctgtcag aaaagaagaa
     8821 cttgaaccca tttcttggac tttgttccaa tatggtcaga agctctctgc cgctacttac
     8881 atcgattctc tgcatgtatg ggatgaagca gcaattgtca tggaaaacct acatcaagaa
     8941 tacgatctat tcctttcacc aacgactgca tttaccgccc ctaaaatcag cgaagatttg
     9001 caaagtgatg cgataagagc acagatggaa cacatcagtg attgttctga agtagaaggt
     9061 ttagacgttg tttcttccat gtttgaggag agcttgaaga tcacacctta tacacagttg
     9121 gctaatctga ctggacaacc agcaatcagc ttgcctactc atttgacaaa agaaaacatg
     9181 cctctaggaa tccaatttat ggctgccaga ggaagagaag atttgctatt tcaaatcggc
     9241 tatctatttg aagcaaatca tcagttccat ttaccaaatc catggaaata gcagaaacaa
     9301 ggagaagaag aatgaagaaa aaaagattgt ttgctctaat aactagtgcc ttttttattt
     9361 taagcgcttg cggtacagct gccgctaatc aggaagataa cagctggagt aatgtagaaa
     9421 aaaagaaaga attagtagta gcgacttctg gcacactgtt tcccgcatct tattataatg
     9481 atcaaaatga attagttggt tatgatgtag atttggcaaa agaggtggct aaacggttaa
     9541 atgtcaaaat ctcctttaaa gaatacaacg tggatggaca aatcacttct gtttcaaaaa
     9601 gagaatcaga ttttgctgcg aacgattttg gtatcacaaa agaaagagaa aaaaaattct
     9661 ccctttcaga gactttcaaa tattcatttg acagtatgat cgtacgaaaa aaagatgatt
     9721 caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc agctggcgaa cccaatacta
     9781 gctatatgcg tttagcggaa aaacacggag ctaaattagt cacatatgac aatgccacga
     9841 atgatcagta tttaagcgat gtagcaaatg gtcgaacaga tgttattcta aatgattatt
     9901 atctgcagaa aatggccgta tctgctttac cagatatccc tgtaaaaata ctggagaacg
     9961 tttatttcaa tgaaaacgaa agtggttttt tattgaaaga aggaaatgat gctctgaaaa
    10021 agaaaatcga tggcgtatta aaagagatga aagaagatgg gacgacaaag aaaatatctg
    10081 aaaaatattt tcaaactgat gtttcagtca aaccgaaaga aacgatggct gagtcggtga
    10141 gtgagtgatg tactttcccc taattgattt ttccctcatg tggacttcac ttcctttcgt
    10201 actgcaaggc ttggtataca cattagggat tggcttcgtg tcgtttcttt taggaaattt
    10261 ggtagggttg cttttgacag ttttaggtct tttggattgg ctgcctctga atgtatttat
    10321 tcgtttttat ttatcgttct ttagaggcat tcccgcacta gtcctgctgt ttctgttata
    10381 tttcgggtta ccttatcaat taagtgcctt aacggcttca gtcatctgct ttacgattac
    10441 cagcagtgca tttattggtg aaatctatcg aggatcacta gcaggcgttt cttcaggtca
    10501 gtgggacgct ggtttagctt ctggtttttc tttctttggg gtattgaaat atatcgtttt
    10561 gccgcaagct tttcgaatct cagtaccggc actcagtaac gtagctatgg atctagtgaa
    10621 aggtacttcg ttagcagcaa caatcacagt gccagaaata tttcaaaaag caaagatcat
    10681 aggcggtcgg acattcgatt atttttctct ctatatttta gttgcactga tttattgggt
    10741 catttgtatc gggatcgaaa aagtgcaaca gtatttagaa aaacgttcta tccatcagtc
    10801 aatctgaaaa atagaaatgg tgctagcgtg gctggcacca tttctatttt ttgtttcttg
    10861 tatattctgc ctagtatttt aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg
    10921 gaggtagaaa acatgaagac cccgctaacc atcgaaatag aatcctcgtt ataccaatat
    10981 tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt
    11041 gtagatacgc ttagctgctt gacaaaattt gatggaagcc gagaatggcg atgctatgag
    11101 ctgaaagtga caaaagcaga ttttcgctct acagccaaac tgtcatttgt tggacattat
    11161 aattattttg ttttaccaag taaactatat gaagaagtaa aaactgaaat ccctacagga
    11221 ataggtgttt tagtgtatcg tccgtacacg gttgaagagg agatgcctgc ctctggtaca
    11281 tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg taccggaaga agctttgaca
    11341 aaccgcttca tggcttcttt attcagggaa gtaagaaaag caaagcaaat ggagcgaggc
    11401 gtaaaatatt ttccaaaaga tcaattgtac aaagaattga aaagacgttc agaaaatcaa
    11461 gatccatttt ctaacgaacg atattatgaa cggtttttag aggaaatagc tagtcaggca
    11521 atcaatgagt tgcaagaaga agttatcgcg cttcagcatg attatgaata tttgaggcaa
    11581 caacagcaga tccgtcggtt gcctacagag ccattggagt gaaaaatatg tactatcctt
    11641 atttaagagg aaaacaattt gacctattag cattaaaaga agcactgtcg cgaggattgc
    11701 tctccaataa aatccaacca gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg
    11761 tgatcgaatt atttcaaaaa aagcaccatc caatcgctgt gatccaaaat ccccaagtag
    11821 gacaattcaa attattcgat cagcatgtgc acagttggac agtcaaagaa aatagctctg
    11881 tcgtccctgc acagatctta acgccagaaa atctcagtga agtactggat tcacctccag
    11941 cgtttcttgt tttcgatgga cagcactacc caaaagatac agaggtgtgg aaacagttgg
    12001 caggagttga ttcgaaattt ttgataccag atactagtcg ctttcgcatc tggctgcccg
    12061 aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa gcatgttgaa agttatgcag
    12121 ataaaacaga tgactttttt agtgatgact atcttttttt ccacgcagac ggatatattg
    12181 gattttctga tttcacgatc gaaggtagcc gttatttcga taaagggttc cctagccgag
    12241 cgcttgctat ccatctaacg tatattgatg cttacggcaa tatacgcgtg aaacattttg
    12301 tctctgacag taacgactct gctaaagacc aagcattgaa gtttcttgaa gcaggagata
    12361 aaatgaaaaa atggatcatg cgtcaccatc atcaactctt gattacttca ggaatgattg
    12421 aattattggc tctttaccag caacaaaaat tccctggact gggagtgttg aaaaagtggt
    12481 cacttatgca tcatttggaa cttattagtc acctgctaga tcatccaaaa gattggctta
    12541 aatattattc aaaagttcag gaactatatg aagtgatcca atgattagat aatgtaaatg
    12601 aaaaaggaat agaaaagagg aaactggatg tatattcggc aaacaaggaa agaagaagta
    12661 gaagaagtgg tagaaatcat taatgccgca gcggatttca ttgcttcgca aggctctcct
    12721 caatggcaaa acgggcagaa accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa
    12781 gaaagctatg tattaatagt ggaagatcaa attgctggaa cagctgcatt agtctcaggc
    12841 gtggatcccg tttacacaaa aatagatggt agttggaaag aatcaactgg tccttattta
    12901 tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa aaaaactagc aaaaccgttc
    12961 ttagaacatt tgttgacgat tgcagccagc aaaaatatcc aagatatccg tatcgatact
    13021 tatccagcta atgaaccgat ggaaaagacc atctattctg ctggattcac ttatcaaggg
    13081 atgattgaat ttccttttga acacggcgaa agaaaagcat atcaaataat catcaattga
    13141 ctataaaatc atctgagcaa agataaagac attaacacta ctgaaaaaaa caatcatatg
    13201 atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa
    13261 atgcttctca aatttttgtg gaattttagc gtattttacg aaatagctgc tttctgaaac
    13321 tcccttcatc gagaggtcgt gacatagctt ttgtcacgat ctctcggctt atcctacgag
    13381 tattattcga tacatttata tcaataatcc tttttttatt ttatctgcag cttcttggaa
    13441 cgctccatcg tttgacacga ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc
    13501 taaatcttct acataaaccg ccagtctggc tgaaatctgt tcagatgaat ctccacgttt
    13561 tttcaggcgc gattgcaatg tttctttaga gatcgtaata aagataggac ggactaccgt
    13621 cccgaaatac tcatataaat gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc
    13681 tactgtcagt ttctttttaa tttctgcttt actggaacca tatttatgtt gagcatattg
    13741 gtcccactct gcaaactcat ttcccttgat catctgattg aatgtattgt cagaaacaaa
    13801 ataataatcc tcttgatctt tttccccagg tcttggtgat cgattcgtgt aagtaatgat
    13861 cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt tttccagaac cagaaggacc
    13921 gataaataca tagcaataat tcattgtcat ttctcctttg atgattgaac aagtgaaaaa
    13981 gctttggaaa atatttccaa agcttttaaa agtaactttg cttttttgta ttttatacca
    14041 gcaagccagt cacaatagca cttaaaaagc tgactaaagt cgctgttagc aaaattttca
    14101 atccgtgttt tgcgacaaga tttccttttt cttcatttaa tcccttcatc gcaccagaaa
    14161 tgatcccgat agaagagaag ttggcaaatg aaacgaggaa gacagatata attgctgttg
    14221 tacgctcagt aaaatggaaa ttaccatttg ctaattctgt catagcaaca aattcatttg
    14281 taaccagttt agtcgccatg atactaccgg catccactgc ttctttccat gggattccgc
    14341 tgatgaaggc aagaggggca aagatgtaac ctaggatctc ttggaaagtg atgccgaata
    14401 tcccgtggaa gatagcattg atcatggcaa tcaaagcaac aaaaccgata agcatagcag
    14461 caaccgtgat cgccacatgg aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg
    14521 tctgttcttt gttttcttca atgatcagct catcttcttt ttcttctaat acatagggat
    14581 ttacaattga agccagaata taaccgccaa ataagttcaa aacaagggca gtgatcacat
    14641 atttagaatc gatcagtgcc atataagatc cgacgatcga catagaaact gtagacatag
    14701 ccgatacact catggtgaac aaacgtttct cagataggaa gggtaattcc ttttttatgg
    14761 aaataaaaac ttccgattgt cctaaaatag cagaagctac tccattataa gattccaatt
    14821 tgcccatccc gttgatttta cttaatccta gaccaacagc tttcataaat aatggaagaa
    14881 tcttgatata gcgtagaata ccgataatag cagaaataaa tacaattggc atcaatacgg
    14941 aaagaaagaa aggtgcactt cctttatttg ctacaccggc aaaaacaaag tcaacacctt
    15001 gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa tacagaaaca accgctgtac
    15061 caaaagttgt acgtaataat acgaaaccaa gaacaagctg taaaacgaac atcacaatta
    15121 gtggtttcaa tttgattgct tttttgttac tgctgatcaa aaaagcaagc cctaatgtga
    15181 caaataaacc aataatacct aataaataac tcaaataatc actcctattc caaattccgt
    15241 tttattttac ctgttttgca aataaaatac taatctttag tgaaattttc attacttttt
    15301 tagagtgaaa cgcattcatt ttccattaga aaataaagcg aacaaaataa aaggtttaaa
    15361 aagtgtaacg tcttagtaaa gtctataaaa aaacgaaact atcgattaag ctaatagttt
    15421 cgttttcaac atttataagt aaactttttc tattggtaat atagattttc ggaagggtag
    15481 acaggtgcgc aggtaatatc gattcctagt tttttcaatg tttgttcatc atttttattg
    15541 accatcacag tagagtgggc ttgtgctccc tcaagttcag ctagtttatc ataagcaact
    15601 tgagcgattg gattcgttac tgcactgatt gataaagcga tcaagacttc attgatgctt
    15661 agtgcatcca aagaggaatg aagtgtcttt gatttcaatc cttgtattgt ttcaagaact
    15721 aatggtgaca ataaaggaat gtcatctgcg atatgggcta aatgttttac tgcatttaag
    15781 ataactgccg ctgacgcatc catcaatgaa cttgtacgcc cggtaatgat gacttcatct
    15841 ggtaattcga ttgcaatcac agcagaagga tcttttgtag gactttgcgc ttgaatctta
    15901 tctgcatagt catgggcagg gactaccacg tttcggtctt caggacgtaa accgacttct
    15961 tccataatca gtttgattcg attaactgct tcttcgtcga tcaatccttt tttgaagtcg
    16021 cattctgttt ggaaataacg acggatgatt tcttgtttag aggcttcttg gacaacggta
    16081 tcatccacga tcccaaatcc tacacgattc acacccatgt cagtaggaga ttgataaaca
    16141 gattctttac cagtaatttt ttcaataata cgtttgatta caggaaatgt ctcaacatcc
    16201 cgattatagt tcacagctac tttattgtag gcatcaaagt ggaaggaatc gatcatattg
    16261 acatctttta gatcgactgt ggctgcttca taagcaatgt tcagcggatg tttcaacgga
    16321 acattccata ctgggaaagt ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt
    16381 tcatgataca gttggttcaa gcaagtagcg agctttccac ttcctgggcc aggagctgtc
    16441 actacaacga ttggtttagt tgtttcaata tactcatttt ttccaaaacc atcttctgag
    16501 acgatttttt ctacgttgac tggatagtct tcgatttctg agtgtttata gaccttgatg
    16561 tttcgtcttt ctagtttatt gataaagact ttcgtggcag gctgtccact atagcgagtg
    16621 ataacgacac tgttgattaa taagccataa ccacgtaatt catcgattaa ccgcaagata
    16681 tccatatcat aagtaatccc ataatcacca cggatcttat tacgttcaat atctccagca
    16741 tagacgcaaa tcagtatttc agcttgatct ttcaattttt gcagcaattt gattttcgca
    16801 tcttcttcaa atcctggaag tacacgtttc gcatgcttgt cgtcaactaa cttcccacca
    16861 aattctaaat ataatttgtc atagtggttc actcgttcca aaatatacgc tgactgctct
    16921 tcaatatatt tttctgaatc aaaaccaatt tttttcacta ggttccctcc ttaagtacta
    16981 aagcattttt tcattttttt gaggattcgt caactggaag agcgaaattc ctcaaaaaaa
    17041 caaagaaaat ggtttaaacc aacttgtcta gatagttatt cattggtatg atagtttttg
    17101 ataaggaagg tgctacatga aaaaaagaca gaaaaagaaa aacgcatata aacactatat
    17161 ccgttcgatt tttaccggtt atgaaagaat gcttgaagat cccgaattgg aacaattgac
    17221 gtttacttat ctaaacgaag aaacgcagct aactcgtgat gagcatcagc gcattcattt
    17281 cacaacaaga gatctgccta gtaaataaaa aataagataa aggtcatcgg attcgattct
    17341 gtcctatcag atggaaagac tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag
    17401 cctgttttat ggagcattgc ccgctctttt ttataacaat tttttacaga aaatggtact
    17461 attgaagtat taataagtga aaaacagatg agtaagagga ggcaagtagt atgaaagagc
    17521 cagatacaaa tgcaaattat gagccagacg aactagagga gacgaacgcg gatcaggata
    17581 caggacaaga atcgtcttct ttttacgata tcaaagaaaa agcaagcatg aatctttccc
    17641 aattattaga agacttacag gcttttgaaa aagctgtccg cgaagaagat atccctgaga
    17701 tctaccgttt atacaatggc cgattgaatg aagaattaaa aagaagctca aatgaaaatc
    17761 acgagatcga tcaattgctg atgcgtaaga tccatgatcg ttttttacag gaatttcctt
    17821 ttatggaaca agtagaaaac atctcgccaa cgatgagcta ttacaaaatc ggcacttatt
    17881 accatgatcg gcccacggtc ggtattgatg caagtctgcc agaaattttt gttttgccta
    17941 agatcgatga agagtgggag aaatattccc aaccggagtc aacggactat gacaaaaaaa
    18001 tcaatgagct agacgcaaag gtgatcactg ctcagacaga aattgaacgt ttagatgaac
    18061 aaataaaaga aatcaatcga caaatgactg atttgaacga caataaagga ttcttgaatc
    18121 gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa gaagcaagta ctatctaatg
    18181 aaaaattagg ctggcttcct tatattgaga cgcctgaaac aatccagcag caaaaagaag
    18241 cattgaagca agaagcaaga gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa
    18301 ggcaaatcag acgctatttc ggtagcaaag agggattcgg tcaagcaatc catgaattct
    18361 tgatgaatta tcttggcaat gagaatccta ataatcaatc taatgaagga ggttccgagt
    18421 atgaatgaac acgatgaagc agaatggaca aaaaatgaag aacaaacaga agatcatgaa
    18481 gaaacgaaca cttcattgga agaacaaaca agtaatcctc tggatgacac agaagaaatc
    18541 cctatgctag aggaaacaaa aatacatgaa aaagaaaaag taactccgac gaatacgaat
    18601 actgcagaaa acacccatgc ttacgaaata actgaaaatc ccgggtattt agaaggaaaa
    18661 gagcaagatc atcaagatat cgtcagtttg attgaaacag cggaagcaga attagcggat
    18721 ttacgtatga gaaaagattt gatcgaatca gattttccac aattattaga atcatatgat
    18781 cgttttatat tgaatgaaga accaatctcc acgatcggaa agacaagcct gatcgaatac
    18841 ttcacgtatg aattactgaa accgttgaat gatattggtc tggaacggtc aaattcagct
    18901 tatgacacat ggaaaacaag acatttttcg atcaattata cgttgaatgg acaaaacgaa
    18961 ttagtctttt cctttgtatt accgaccatc gatcaacgat accaagtgga atcgatgagt
    19021 ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc aagatgaccg ggtgctttct
    19081 ttgatccgac actggtcagt ggatcgagta ttctctgcag ggcagatcac gatattcaat
    19141 cacaaattga atcaattatt agcccatgca agaacacttg gttttaccgt taatcagaca
    19201 ttactagaca atacgaagcc acttcgtttg aatcttcaga gcgaatttga attgacgaat
    19261 gaagtattgg atgatatttt tatcgtggca atgaaaaatc catcctacga tatggaaaag
    19321 atcaacgaaa ctacttatca agtacgatta gataaaggac agtctttaac gataacaaca
    19381 aacgaaaaag atcaaacagt cttgtcgatc tcatctggag actacccacg atctgtcatt
    19441 gatttcttta tcaattacga attcttggtc ccattaatgg tgagaaagtc ctgattggat
    19501 aagttggatt accagatgtc attcagataa ttctgttcca aaggacttgc ggcagttatc
    19561 cagaataatt gtcacaagtc ttttctgtat gtactaaatg gacgtgaata acttgctgat
    19621 gaaggaggga tttcattagg aatgtccaca aataaaatag cacagcttag cttattgtct
    19681 gctgcttgtg tagcaggtag gatcgctttt caatttattc cgaatttcca gccaatgact
    19741 gctatttttt tatttatagt actttactta agcttaaaag atgcgttaat tgtcatgagt
    19801 ctttctattg cgatcagcag tttttatttt ggtgttggac catgggtcat cggccaatgg
    19861 atcagctata ctgttgttct gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa
    19921 aggaatctat ggttaaaggg agtctgtttt tttctggcgg gcatggttta cggaataggt
    19981 atgaccgttt ttgatacgct gttatatcgt ttgcctcaac cttggatcta ttaccttcaa
    20041 ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg tgttctttct tgtttttctg
    20101 ccagtcgtca aacgttttta caatcattca ggaggaaaaa atgaaaaaaa taatttatag
    20161 tttgcttgca atgagtgtca gtatactcat tttgacaagt tgcagtacaa cctatacaca
    20221 atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca
    20281 agaaaatggt caaaactttt cggaaaaaga agtggtcttc aaaaaaggcg atgatttact
    20341 gagtattttg aaacgaaatt ttgaagtcaa agaagataac ggcttcatca cttcgcttga
    20401 agggcactca caggatgaga aaaatcaact ctattggatg tttactgtgg acgggaaaag
    20461 tgctactact ggtgccaaag agatcaaatt gaatgatgga caatcggtca ttttcaattt
    20521 gtctaaacta taagtttcat aaaaaactgt taaagggcga atattgccat aattcagtga
    20581 gttcagttga tgaaagaagg aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc
    20641 cgtaattgct gctctagggg ctaatattct agttgctatc agcaaatttg tcggctatgc
    20701 aatcagtggc agtgccgcca tgctaaacga aagcatccac agtatcgttg attgcagcaa
    20761 tcagattttg ctgttattcg gtgataaaca agccaataaa ggacagagcg agttgcatca
    20821 atttggtgaa ggacgagcaa aatacttttt cagtacaatc gtcgcaatga tgcttttctt
    20881 cggcggtggg gcacttgggg tgatggaagc ttttgaaaag ttgacgcatc catcccatga
    20941 agtgggtaat ccgatcattg ttatagcaat actgttattt ggtttggtaa tcgaaggaag
    21001 ttcgttacgc gtggcaatga aagaaatcaa agaattgaat actgaaaaac tttccacgat
    21061 gcgctttttg cgcgaaagtc gacacagtga aatactgatt atttttacgg aagacttgtg
    21121 tgctgtaatc ggtttggtct tggctttagc aggaacaata ttgacttatg caaccgggat
    21181 tgctgcattt gatgcgatca gcggattgct gatcggtttc ttgctgatgg gagccgccat
    21241 tttcctagca aaagaatttt atagtctgat catcggtgaa agcgtgacag caagcgattt
    21301 atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt cggctgatcg atataaaaac
    21361 agttcattta agtccaacgg agattttagt tgcagcaaaa gcagatatcg ttggtaacaa
    21421 agagaatgaa gcttactcgg tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc
    21481 tgacaagaag atgtatatat acattgaaac agataaatat gacccaaatt attctcgtaa
    21541 ataaaacgtt tattcatgaa ttgaagcaaa aatgagaaaa atataatctt gtttttaaat
    21601 aaaacggcta caatttagcg aatttatgtt acaatgagtc cgctgtttat ttgttgtaat
    21661 taaaggagat attttaatga acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc
    21721 agtgatgcca ctttgtataa gctatatacc agtaggatta gcttgcggag tattgttgca
    21781 aaaagttgga tttgatcctc tcttatcagg actgttatcg attctggtct tttccggtgg
    21841 cgcgcagttt ttagttgcat ctatgcttac gacgcaagca tcatttgcaa cgacattatt
    21901 gatggttttc tttttagaat tacgctatac attattagga gcaagtcttg ccggttttat
    21961 gaaaaaagaa aagcggacgt ttttggctgt cttttctcaa tcactgaatg atgaaaacta
    22021 tgcggtgaac tacttaaagt tttcaactga cccttcctgg aataaaagaa aggctttata
    22081 tgtcaattgg ttctcaatga ctgcatgggc cggtagcaat atgatcggta attttttcgg
    22141 ctcggtgatt cgagtagatg ctgaccttgt ccattttgct ttgacggcca tgttcatttt
    22201 catgtttatc atgcagatga aaagcgcact actgatcttt acaggtttat tctcaggcgt
    22261 attaggggtc gtattcatgg tattattcca aaatactttc ggattgattg cagcaacagt
    22321 cattgcttca tttgccggat ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa
    22381 actaagagac cgcggtaaag atgtcaatga gccgctcttc aacttcccgg aacaggaggc
    22441 caatcaccat gactaaacta tatttgctac ttttagttgc ttgtctgttc atcgtggcat
    22501 atatcccgcg cttgttcccg atgctttatt ttactcatcg aaaagtgcca tcctggttca
    22561 gtgactggat gaaatatgta ccggtggcat tatttgcggc gttagctttt aaagatgttt
    22621 tcattactca tgaacatctg gatattgctt ggaatatcaa aatagctgca atgattctag
    22681 tagcaggcgt ggcttacaaa acacgttcga tggcgttgtc agttttgaca ggacttgcgt
    22741 ctgtcttttt gctgtctatg ctttaaactg ttttatttta ttaaaaacag agattgcgac
    22801 ataagttgtt caaactgatc tatccgaact gcatttgcaa cagtcaattc tggatgcaag
    22861 tttattcgta gggataaaaa agattagtgt tggcttatga tgcaagctct tttttatttt
    22921 aggaattaga gagggatttg tccttctatg attttttgct ataataggga agatgtttat
    22981 tttatgaaag aatagttgag ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt
    23041 ggctaacctt tgctaatctt ctatgtaaag ttttaggggt agtctatttg attccctggc
    23101 taagtatgat ggggaacaat caagatggta tgcttgctac gacattgtat aatgttggct
    23161 atcttcctta tggattgttc ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa
    23221 aaaaagtagc tgtagccaca aaagaaggag ataacgctgc ttgtcggctg atttttagaa
    23281 gtacgatcaa tatcatgttt gtcatcggta tcgcctctgc tgcactgatg tatttatttg
    23341 ctcctttact agccggaatc agtccgattg ctaacgttaa taatgggata ctagctatca
    23401 gaagtttatg cccatctttg attgctatcc caattcttag tgcaatgaga ggatatttcc
    23461 aaggaaaaaa tgatctgcgg ccttatggaa cttctttgat tatcgaacag gctgtgcgag
    23521 tgatcgttat cttggctggt acttattact tgcgcgtatt gactgacgga accatattag
    23581 aagctgtatt gatcagtaca gtcgcttctt tcttcggtgg gttagctgcg attatccata
    23641 tgtatatcgt cggacgcaga aaagacttct tcgaacttaa agattttttg atttccagcc
    23701 gttatttcga aagagaaaac cgatctgcct ctgtctctat cattcgggaa acactaccgt
    23761 ttatttttgt cggctcagtc attactatcg ttcaaatgat cgaccaagta acgatgaaac
    23821 cactgcttca ctttttccgt ccagagatcg cagatcagca actggaattc ttattcagcc
    23881 gtgcttctgt caatccaaat aaattgacat tgatcctgat ctccatggta ggaacagtag
    23941 ctataagcag cttgccaata ttaagcacga tgaagaaaaa agatcggata caaatcgaaa
    24001 aaacagtagg agatagtttt tcgattgcgt tattgattct tcttccttca ttgactggaa
    24061 tgtctttatt agctggacca ctatacacat tgttttttgg ctatgatcct gaaagtgtcg
    24121 gatatttcca aatggctttg ctagcttctt tgtttttctc tttattcacg atcctatcta
    24181 cgatgcttca atctttgaat caccatcgtt ttgcgatccg attaactgta gaagcaatta
    24241 tcctgaaagt catcttccaa gtaatcggat taggattagt tggcggatac gggatgagct
    24301 tatcaaatgg cttagcattc ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag
    24361 aataccgaat agcaccttta gcaaagatca gcaatttttt cttaaaaact ttccgctcca
    24421 ccctgattat gcttgcagta tgttttgccg ttttcttttt gctgaatcag cgattgtcaa
    24481 tggaatcaaa aagctatgca atgatttatt gtgtggctgt aggaagtatt ggcggcttag
    24541 tatttttatt ttctcaattc ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc
    24601 atagcagaag caaagaatag gttaactaat cgttttttgc tgtcacttga aaaacggcaa
    24661 ctcgtatgat tacgcttgtc gtttttctat tttccaattt tagtttcata tctaatctta
    24721 cttatgatat agttataagg tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga
    24781 aacttgcagt agttacagat agttcggcat atctgccgga acgtatcaag aaccatcccg
    24841 acttattcgt tattccaatc cctttgatta tggatgggaa aatttataac gaaggaatcg
    24901 atatcgaagc ggatgaatat tatggacttt taaaaaacag taaagatttt cctactactt
    24961 cacaacctgt cgtaggggaa gttttgggtt tatatgagga attgaaagag aaagggtatg
    25021 acacgatttt aagtatccat ctttcttcag ggatttctgg ctttgtcaac acattatttg
    25081 cgatgaaaga tgacatcagc ggagtaacgg tcattcctta tgactctaag atcactagta
    25141 tgccgatggg acacatggta gaagctgcct tagacttaaa tgatgaaggt cagtcccttg
    25201 atgaaatact tgcacatatc gatcgaatcc gagataatac ctatgcttat ctgattgtag
    25261 atgatttgaa caatttagta cgaggaggac gtttgacaaa tggtgcagcg ctgattggca
    25321 gcttattaaa aatcaaaccg atcctgacat tttctgaagg gaaaattgtt ttattcgaaa
    25381 aaatccgttc aagtaaaaaa gcatttgctc gagctgaaga aattattggt agaagaaata
    25441 acgaaataca accgccagtc aaactttacg tgatacatgc aaataattta gaagtagcga
    25501 ttgcggagaa agagaaattg cagaaaaaat atccaaatgc agaaatcgaa attggtcatt
    25561 tcggaccagt cattggtaca catttaggtg aaaaagccat cggcattgcc atttcagcgc
    25621 aataattcct tttattcaaa aaacatacca aaaata
//
LOCUS       DAZUMB010000029        25513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000029 DAZUMB010000000
VERSION     DAZUMB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.029"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..308
                     /locus_tag="ACG80L_001002"
     CDS             <1..308
                     /locus_tag="ACG80L_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5023277.1"
                     /translation="IRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLI
                     EGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            360..530
                     /locus_tag="ACG80L_001003"
                     /pseudo
     CDS             360..530
                     /locus_tag="ACG80L_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(703..1176)
                     /locus_tag="ACG80L_001004"
     CDS             complement(703..1176)
                     /locus_tag="ACG80L_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5023278.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1188..2135
                     /locus_tag="ACG80L_001005"
                     /pseudo
     CDS             1188..2135
                     /locus_tag="ACG80L_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2406..2841
                     /locus_tag="ACG80L_001006"
                     /pseudo
     CDS             2406..2841
                     /locus_tag="ACG80L_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            2857..3690
                     /locus_tag="ACG80L_001007"
     CDS             2857..3690
                     /locus_tag="ACG80L_001007"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5023279.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            3935..4960
                     /locus_tag="ACG80L_001008"
     CDS             3935..4960
                     /locus_tag="ACG80L_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5023280.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5098..5223
                     /locus_tag="ACG80L_001009"
     CDS             5098..5223
                     /locus_tag="ACG80L_001009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023281.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5248..6363
                     /locus_tag="ACG80L_001010"
                     /pseudo
     CDS             5248..6363
                     /locus_tag="ACG80L_001010"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6446..7342
                     /locus_tag="ACG80L_001011"
                     /pseudo
     CDS             6446..7342
                     /locus_tag="ACG80L_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7358..8182
                     /locus_tag="ACG80L_001012"
     CDS             7358..8182
                     /locus_tag="ACG80L_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5023282.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8214..9524
                     /locus_tag="ACG80L_001013"
     CDS             8214..9524
                     /locus_tag="ACG80L_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFS5023283.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9672..10931
                     /locus_tag="ACG80L_001014"
     CDS             9672..10931
                     /locus_tag="ACG80L_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5023284.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10941..11813
                     /locus_tag="ACG80L_001015"
     CDS             10941..11813
                     /locus_tag="ACG80L_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5023285.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11825..12655
                     /locus_tag="ACG80L_001016"
     CDS             11825..12655
                     /locus_tag="ACG80L_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5023286.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12695..14878
                     /locus_tag="ACG80L_001017"
     CDS             12695..14878
                     /locus_tag="ACG80L_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5023287.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14939..16159
                     /locus_tag="ACG80L_001018"
     CDS             14939..16159
                     /locus_tag="ACG80L_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS5023288.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16156..17616
                     /gene="gtfA"
                     /locus_tag="ACG80L_001019"
     CDS             16156..17616
                     /gene="gtfA"
                     /locus_tag="ACG80L_001019"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFS5023289.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17693..18799
                     /locus_tag="ACG80L_001020"
                     /pseudo
     CDS             17693..18799
                     /locus_tag="ACG80L_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19121..19330
                     /locus_tag="ACG80L_001021"
     CDS             19121..19330
                     /locus_tag="ACG80L_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5023290.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19323..19472
                     /locus_tag="ACG80L_001022"
     CDS             19323..19472
                     /locus_tag="ACG80L_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS5023291.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19626..20291
                     /locus_tag="ACG80L_001023"
     CDS             19626..20291
                     /locus_tag="ACG80L_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5023292.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20575..21660)
                     /gene="xerS"
                     /locus_tag="ACG80L_001024"
     CDS             complement(20575..21660)
                     /gene="xerS"
                     /locus_tag="ACG80L_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS5023293.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            21994..22227
                     /locus_tag="ACG80L_001025"
     CDS             21994..22227
                     /locus_tag="ACG80L_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFS5023294.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22224..22631
                     /locus_tag="ACG80L_001026"
     CDS             22224..22631
                     /locus_tag="ACG80L_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5023295.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23768..24286)
                     /locus_tag="ACG80L_001027"
     CDS             complement(23768..24286)
                     /locus_tag="ACG80L_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS5023296.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            24653..24901
                     /locus_tag="ACG80L_001028"
     CDS             24653..24901
                     /locus_tag="ACG80L_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023297.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            25404..>25513
                     /locus_tag="ACG80L_001029"
     CDS             25404..>25513
                     /locus_tag="ACG80L_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5023298.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         8680 a         3931 c         4739 g         8163 t
ORIGIN      
        1 aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt aaattgctca
       61 tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga actatttatt
      121 caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa agaaaagagc
      181 aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac aactataatc
      241 aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca ttagaatcaa
      301 tgttttaact tgatcaacag aatgggcttt tccatttaca caaaattatt gacgctccca
      361 cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga gacattgcta
      421 ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac attggtataa
      481 ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa tttaaaaatt
      541 tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat tttatttcat
      601 aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt gttatataaa
      661 ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa agagaatcaa
      721 tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact caacgtttag
      781 cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt tgaccttctg
      841 caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa ttgccgtttc
      901 ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt taatatctgt
      961 atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga gcagactaca
     1021 atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt taatgatgat
     1081 tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg atgaaaacaa
     1141 aagttgttca ccatctttta taggtttgat tctcattttt agatattatg cccgcacgta
     1201 ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt tatcataata
     1261 tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc atgtatcacc
     1321 tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt gaagtacaag
     1381 aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta aaagttcctt
     1441 ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt tgccattatg
     1501 gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat gcaacacgat
     1561 tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg tatgtcacag
     1621 accagatccc tacagagcat gccattttag gaattgatgc cattcaacta cgcgtacaat
     1681 attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa gaatacgtca
     1741 gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact cagttctatc
     1801 aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg attggtagcg
     1861 tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc gacaaacaat
     1921 tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt gtatcacttt
     1981 attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga aacctagatg
     2041 caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt ttaccgcagt
     2101 acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc catgacgaag
     2161 agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc cattctaaag
     2221 aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta ataaaactac
     2281 ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt aaactaaaca
     2341 tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat aaaggggagt
     2401 accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa aaatgtcagt
     2461 ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc aggaaaatca
     2521 tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga cacctatgat
     2581 agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga gattgataat
     2641 ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg ctcaacgtta
     2701 gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt tgcaacacct
     2761 agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg ttctgtctgt
     2821 cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc ggctattgat
     2881 aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg aattgaggaa
     2941 caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc agaagatgtt
     3001 aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt acgtgtaaaa
     3061 gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga tacgcatctt
     3121 tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt aaatacaaat
     3181 gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca tgagaaaata
     3241 gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga acgacatcaa
     3301 gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg gatatataac
     3361 aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat tgttgttact
     3421 ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa acgagtgatg
     3481 gaacaattat cgattattgg atttgatgat attacttttg cagaattaat ggatcctgcg
     3541 ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca ccaactgatt
     3601 ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg agaattgatt
     3661 tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt atagttaaca
     3721 ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc gtcgcaaatt
     3781 cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt ttttaattat
     3841 gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga ttatatgtta
     3901 gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg atgttgcaaa
     3961 acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc cgacactttc
     4021 agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc attatgtcaa
     4081 aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag tttctgaagt
     4141 agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag tcgaagcaga
     4201 acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt tggagcaaga
     4261 cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt ctgtgattga
     4321 agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta ttgaagctga
     4381 agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag atcgattgta
     4441 taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt tagatagcca
     4501 aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga cgtggatgaa
     4561 acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca cacttgacgg
     4621 aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc cgactgctat
     4681 catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa aaaatggtct
     4741 acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg ctgaattttt
     4801 aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac tagcagttcg
     4861 catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga ttgtttctaa
     4921 taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac atccaaaaaa
     4981 gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa aacattaagt
     5041 aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg aattaagatg
     5101 aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac attactcgca
     5161 gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag ctatgcgagt
     5221 tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga aaaacaaaat
     5281 cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt taaattagaa
     5341 gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag cgaatcctat
     5401 aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga aaatgatatt
     5461 gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga cgaactatac
     5521 gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt atttgataat
     5581 gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca agctgcaatt
     5641 gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg aacagagact
     5701 ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga caatacaaaa
     5761 tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga tattgcactt
     5821 gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt aagtcgtttt
     5881 caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg atttttgaaa
     5941 aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa agttaatgca
     6001 acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc agaagaagtg
     6061 aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc ggaaacgggg
     6121 gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc agatacaatt
     6181 gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata caataaagat
     6241 ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc tggtgaagta
     6301 actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt gagcggaaat
     6361 taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg tatattaatg
     6421 gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat atcgtaaaga
     6481 tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa ttttattaaa
     6541 tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag ggttcggtga
     6601 tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg aagtttttca
     6661 gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg ttgtattatc
     6721 cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt atcggacaat
     6781 ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc gctggatgtt
     6841 caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg atggaccagc
     6901 atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag tttggagtgc
     6961 tattggttat aacatggtgt tattactagc cggcttataa gaaattccta aagattatta
     7021 tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca ttacgttacc
     7081 attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg ctttccaagt
     7141 atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta aatcacaatc
     7201 attagcctat ttattctata aacattcatt tattttaaat gacaagggat atggttcggc
     7261 aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa tgaagttgca
     7321 gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat ttttttcaaa
     7381 tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac cttttatttg
     7441 gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac ctacaatttt
     7501 ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac cattcatggc
     7561 tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg ttttattttc
     7621 agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata tccttttcag
     7681 cttagtactt attcaaatga tgattcccgg ttccattttt attactccac agtatttatt
     7741 agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag gggtagttag
     7801 tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg aattggagga
     7861 agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta tgttgccgct
     7921 tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat ttaaagattt
     7981 aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc cgggattagc
     8041 aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt cgttattagc
     8101 aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg aaggaattgc
     8161 tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag tacatgacaa
     8221 aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta ggcgatgcga
     8281 tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat gaaaaacgac
     8341 taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa aatcgtgctg
     8401 atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt gttatcaatg
     8461 ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt cctaaaaaat
     8521 atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt agaaccctac
     8581 ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt ccaaatgctt
     8641 ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta aaagcgacga
     8701 gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa ttgtttgagc
     8761 atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt gcaggcatta
     8821 atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat ccaacaattc
     8881 gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt gaattgttga
     8941 aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac catccatact
     9001 ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg gatgaatact
     9061 tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac attgtaaata
     9121 atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg gattctatta
     9181 cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta atcacaaact
     9241 taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc gtgcaaccaa
     9301 cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat atcaatgttc
     9361 aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat caagcagcgt
     9421 atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat ttagtggatg
     9481 atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa gctgaaagag
     9541 taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat attgacaaaa
     9601 cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa ttgtataagg
     9661 aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca ctaggagcaa
     9721 cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag gtagaaatag
     9781 agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct aaagattttg
     9841 aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct ggtgaagtaa
     9901 ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat cctcaaagta
     9961 tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa gaaccgtatt
    10021 tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt gtatatagca
    10081 taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt gaggaaatgg
    10141 ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag attaaagatc
    10201 aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat ggttatcacc
    10261 aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt tggcgtttct
    10321 cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt aatcgattag
    10381 atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt tataacgata
    10441 cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca tgggcaatgc
    10501 cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt ccagcagatg
    10561 aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt tctgcaactt
    10621 ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct gaagcgatgc
    10681 agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc gagaatactc
    10741 aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat ttagtttggt
    10801 tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat tatttacaca
    10861 atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca atgaaagctg
    10921 acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac tgggcaatca
    10981 tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca atggttcaag
    11041 gtgcttttta cagtttaact aattggactg gtctgactta taattttgat tttgtgggtt
    11101 tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt ggcttcactt
    11161 taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg attgcgcggg
    11221 cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt tttccagcgg
    11281 tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat ggtttaccaa
    11341 aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga acaacatggg
    11401 gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg ccagtcatca
    11461 ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct agaatggatg
    11521 gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta ccaagtattt
    11581 caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa atttttgctt
    11641 taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt tataattatg
    11701 cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta tttatcttaa
    11761 ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc taggaggttt
    11821 cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat tattaggggg
    11881 cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa caacacccga
    11941 tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt tccgaagatt
    12001 gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa tcattgcagt
    12061 agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa aaatgactcg
    12121 tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg ttcctttcca
    12181 agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa atatgtgggg
    12241 attaatctta ctctatctca cttatgccgt accacagaca ctatttttat atgttggttt
    12301 tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg gggctagtca
    12361 ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg cgacgactat
    12421 tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa ttttaaatcg
    12481 tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac aatattttaa
    12541 cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta cactagttta
    12601 tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga aataaccaat
    12661 aaattattct cattataaag gagttcataa acttatgatt atatttgaca atcacacaca
    12721 aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag aaaataatta
    12781 tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt atcgctaccc
    12841 ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt tattatcttt
    12901 agacacatta ccattagaat ttccaggata tggacatggt gattttagag aaccggcgca
    12961 taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata gctacgaaat
    13021 tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag atgatgatga
    13081 agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt tacagttgtt
    13141 ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag taaataatag
    13201 tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc caaatcgtcc
    13261 actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga ctagagaaaa
    13321 aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc atcaacaaaa
    13381 tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg catatggatt
    13441 taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat ttgaccaaac
    13501 acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa ctggcgactc
    13561 atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata cgttatcaca
    13621 gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt taaaagaacg
    13681 tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag aaaaaatcat
    13741 gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag atgatggttg
    13801 gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa cagaaggcaa
    13861 acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga tgaaatttgg
    13921 gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag cacatccaga
    13981 ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat atgttcttga
    14041 tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga tttttgatcg
    14101 tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag tctattctgt
    14161 cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag ggttatatga
    14221 atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt gttcgggtgg
    14281 aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga cgagcgacaa
    14341 taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt atccaatttc
    14401 aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag aaacgagttt
    14461 aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag atctaacgca
    14521 attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata aaaagcatcg
    14581 ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag gcaatgatac
    14641 tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt tccgagtatt
    14701 agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag aagaagcaac
    14761 ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg gtttttatat
    14821 tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac agatatagta
    14881 gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg aagaaaatat
    14941 gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa atgaagaagt
    15001 acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag gcggatttag
    15061 aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg aatctgatgg
    15121 agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca ttgaagtgtg
    15181 gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg tagcagaagc
    15241 cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt attcgatggt
    15301 ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt ttaaagatag
    15361 ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag ttacgcaaga
    15421 taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa ttaataaata
    15481 tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc tttttatgtt
    15541 actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca acatagaaat
    15601 ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac gggcattaga
    15661 atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc gggataattc
    15721 tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt ctgataatgt
    15781 atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt taaccaatca
    15841 acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata gtgtatacaa
    15901 agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac atattttgct
    15961 ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta cagcaacaga
    16021 acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt atgaagatgt
    16081 tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg tattagctaa
    16141 ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta tgcagatagt
    16201 ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca aggtgttatt
    16261 ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt tgcgccgagt
    16321 gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc attaggtgaa
    16381 gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc aaaatttttc
    16441 caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat ccgtattcat
    16501 gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta caagcgtaaa
    16561 gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga ggtttggaat
    16621 acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa agaatttatt
    16681 cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt agatgcgttt
    16741 gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga tatttgggaa
    16801 cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt accagaaatc
    16861 catgagcatt attccattca aatgaaaatt gccaatcatg attattatat ctatgacttc
    16921 gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg tttagccaat
    16981 tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga tggaattggt
    17041 gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc tgaggaattg
    17101 tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa taatttagat
    17161 atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg tagctatctg
    17221 ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta cgttgggtta
    17281 ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg taatattaat
    17341 cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt gattcaacaa
    17401 ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga tggaacgatt
    17461 gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa aaacggtagt
    17521 gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga gtctaaagat
    17581 ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa caaaaaatcc
    17641 tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat acatggataa
    17701 gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag gaaaactttc
    17761 tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac tgtcttttga
    17821 agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt tgcgtgattt
    17881 gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat cgattagtca
    17941 tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag aaatttttca
    18001 acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc atctgtatct
    18061 gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga ctttccgtca
    18121 aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata ctacgatgta
    18181 tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac taccagtatt
    18241 ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact ttgaaaagat
    18301 ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa acaccaaagt
    18361 tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag acgagctggt
    18421 tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg ttcaagataa
    18481 gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa aagaaatctc
    18541 tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca aacaacacat
    18601 gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat tgattttaac
    18661 gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg aaaaatcagt
    18721 gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat gttgtctaga
    18781 aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt tttcagttga
    18841 ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag ttttttttga
    18901 cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca acactagtga
    18961 tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc aagtatacaa
    19021 agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact atttttagta
    19081 gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta taacaaaaaa
    19141 tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac gacaggcaaa
    19201 agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag gactagtacc
    19261 tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg attttcagaa
    19321 tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct agtttgggat
    19381 tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa cagttaaaga
    19441 agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga aacttctcaa
    19501 agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta tcaagggaaa
    19561 gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt tcttatttct
    19621 ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa aataatttat
    19681 tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag atgtcattag
    19741 atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt ggaaatgtga
    19801 agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa gttatttcaa
    19861 ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg ttacttttta
    19921 tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt gattttgaaa
    19981 ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc tatgagtttg
    20041 tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat acaattacct
    20101 ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt gtactttctt
    20161 ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga catgcaacga
    20221 tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct tctttgctta
    20281 tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct gatgtacaaa
    20341 cgactttagg aacttatggt catttgtatc taaattcatt gagagagata acaaatgatc
    20401 ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt tacgataata
    20461 aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag aaaaactata
    20521 atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt atactcataa
    20581 agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag ttgttgtatt
    20641 ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct gttggtagag
    20701 tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa agccttttga
    20761 atattttgct accatccgtt cgattgcaat accggatata cgcttggctt ctccttggta
    20821 aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt tccgaatagc
    20881 tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat ctcttttatt
    20941 tccttttcta gttactacca cattattctt atctaaagat aggtcatcta aattaatatt
    21001 tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg ccaggtctct
    21061 ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt tatattcatt
    21121 ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt tctcaatggc
    21181 attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat ccataggatt
    21241 attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat atttaaatag
    21301 aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta agttattatt
    21361 atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc gacgtttcaa
    21421 atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg tgatagaagt
    21481 atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat tttttagata
    21541 ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa aatcttttat
    21601 ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg tatttttcat
    21661 gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga ctataccaat
    21721 tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc aaaaaaagca
    21781 tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata gggaaggaaa
    21841 tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga aagaacaatc
    21901 tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca agcgtatata
    21961 ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag aaaatttagg
    22021 gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga aaatgggtta
    22081 aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat tacaaaaaaa
    22141 caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga atatgatact
    22201 acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa gatattataa
    22261 aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt gtaaaggatg
    22321 cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt gaagaggttt
    22381 atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag aaacatcctt
    22441 ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg aaaatcaatg
    22501 gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat attgctacgt
    22561 atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag acacatacgc
    22621 agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct atagcgaaat
    22681 aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa acgtaaattt
    22741 tctatatagt tggattaaga taacgactat ttattttatc atatttttac tataccctat
    22801 acttttactt ggtatattta tgaataatcg caaatttacc cctatactac tagtttgtaa
    22861 gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc tacaacttat
    22921 aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag taaagtttgt
    22981 tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc tgaaaactta
    23041 tgaaatgaaa gaccttttac atcttgcaga aatcggtact gggattaaac taagcaataa
    23101 aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat tctgataaat
    23161 gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga tctcttcagt
    23221 atcagtaata ggataggcga taggccacat accctttgat ggttagaata aactgctcaa
    23281 aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa ttataaacca
    23341 taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca gaagatataa
    23401 ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta aagggaattg
    23461 tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc ccctgctagt
    23521 ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga agtgagtcat
    23581 ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc tacggcattc
    23641 taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga acttatgtag
    23701 tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct tagcagtaaa
    23761 cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa gtcccaaatg
    23821 gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt ttggttgcct
    23881 cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt gaatcaatca
    23941 tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc aatggtagat
    24001 gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat ttaaattgat
    24061 cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt agaattaaat
    24121 tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact tcctcaactc
    24181 ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta gtataagtcc
    24241 tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata ttacctccat
    24301 aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt ttaatccgtt
    24361 ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga tttataaaat
    24421 atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa aatacgaata
    24481 tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta aatatcaaaa
    24541 tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta agaactgtta
    24601 agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga aagtggtaaa
    24661 actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg cgttgggtaa
    24721 aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc agatgaaaca
    24781 gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc gattgccata
    24841 ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc atccttttta
    24901 ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga tgttttcacc
    24961 agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga aaattcaccg
    25021 cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt ccaaaattaa
    25081 gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt ctttttaaac
    25141 aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt ttatgacttt
    25201 cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg gacgtttacg
    25261 agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg aactaccccc
    25321 acttagcttg gataggctac aattaactag acagaaaaat taaggtgtgt agactagaag
    25381 aaaacatacc aggaggaatt tttatgtcta agagaacacg aagaactttt tcacaagaat
    25441 tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa atcattcgag
    25501 aatatgaact aac
//
LOCUS       DAZUMB010000030        25224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000030 DAZUMB010000000
VERSION     DAZUMB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.030"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..776
                     /locus_tag="ACG80L_001030"
     CDS             129..776
                     /locus_tag="ACG80L_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5023299.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    905..1121
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1169..3814
                     /locus_tag="ACG80L_001031"
     CDS             1169..3814
                     /locus_tag="ACG80L_001031"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS5023300.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4124..5437
                     /locus_tag="ACG80L_001032"
     CDS             4124..5437
                     /locus_tag="ACG80L_001032"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS5023301.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5427..6080
                     /locus_tag="ACG80L_001033"
     CDS             5427..6080
                     /locus_tag="ACG80L_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5023302.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6135..6686
                     /gene="radC"
                     /locus_tag="ACG80L_001034"
                     /pseudo
     CDS             6135..6686
                     /gene="radC"
                     /locus_tag="ACG80L_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6817..7902
                     /locus_tag="ACG80L_001035"
     CDS             6817..7902
                     /locus_tag="ACG80L_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5023303.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7899..9791
                     /locus_tag="ACG80L_001036"
     CDS             7899..9791
                     /locus_tag="ACG80L_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS5023304.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9798..10175
                     /locus_tag="ACG80L_001037"
     CDS             9798..10175
                     /locus_tag="ACG80L_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFS5023305.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10329..11111
                     /locus_tag="ACG80L_001038"
     CDS             10329..11111
                     /locus_tag="ACG80L_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFS5023306.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11237..11968)
                     /gene="erm(A)"
                     /locus_tag="ACG80L_001039"
     CDS             complement(11237..11968)
                     /gene="erm(A)"
                     /locus_tag="ACG80L_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFS5023307.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(12026..12085)
                     /locus_tag="ACG80L_001040"
     CDS             complement(12026..12085)
                     /locus_tag="ACG80L_001040"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS5023308.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12477..13139
                     /locus_tag="ACG80L_001041"
     CDS             12477..13139
                     /locus_tag="ACG80L_001041"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5023309.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13374..13517
                     /locus_tag="ACG80L_001042"
                     /pseudo
     CDS             13374..13517
                     /locus_tag="ACG80L_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            13667..13867
                     /locus_tag="ACG80L_001043"
     CDS             13667..13867
                     /locus_tag="ACG80L_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5023310.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14121..15800)
                     /locus_tag="ACG80L_001044"
     CDS             complement(14121..15800)
                     /locus_tag="ACG80L_001044"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5023311.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15802..16014)
                     /locus_tag="ACG80L_001045"
     CDS             complement(15802..16014)
                     /locus_tag="ACG80L_001045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS5023312.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16407..18137
                     /gene="efrA"
                     /locus_tag="ACG80L_001046"
     CDS             16407..18137
                     /gene="efrA"
                     /locus_tag="ACG80L_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS5023313.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18134..19894
                     /gene="efrB"
                     /locus_tag="ACG80L_001047"
     CDS             18134..19894
                     /gene="efrB"
                     /locus_tag="ACG80L_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS5023314.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19989..20186
                     /locus_tag="ACG80L_001048"
     CDS             19989..20186
                     /locus_tag="ACG80L_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023315.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20339..20824
                     /locus_tag="ACG80L_001049"
     CDS             20339..20824
                     /locus_tag="ACG80L_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS5023316.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20928..21521)
                     /gene="clpP"
                     /locus_tag="ACG80L_001050"
     CDS             complement(20928..21521)
                     /gene="clpP"
                     /locus_tag="ACG80L_001050"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS5023317.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21701..22228
                     /locus_tag="ACG80L_001051"
     CDS             21701..22228
                     /locus_tag="ACG80L_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023318.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22319..23056
                     /locus_tag="ACG80L_001052"
     CDS             22319..23056
                     /locus_tag="ACG80L_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5023319.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23060..23977
                     /gene="miaA"
                     /locus_tag="ACG80L_001053"
     CDS             23060..23977
                     /gene="miaA"
                     /locus_tag="ACG80L_001053"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS5023320.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23979..25208
                     /gene="hflX"
                     /locus_tag="ACG80L_001054"
     CDS             23979..25208
                     /gene="hflX"
                     /locus_tag="ACG80L_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS5023321.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8397 a         4111 c         5303 g         7413 t
ORIGIN      
        1 ccgcagatgc aagcttttga acaccgtttt atttgtctca cacccttttc cttcctactt
       61 gtaaccttgc tcccgtgtta gaaacatgtt acactaaata tgtgaaatta ttaacaagga
      121 gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc aactgcgaaa agattacctt
      181 tatatttacg ctacttgaag atgctggatg actcaggtat aagtagaatc aaatcaaatg
      241 agttcagcga aatcacccaa attccttctg caacgatccg cagagacttc tcgcaattag
      301 gcgagctagg cagaagcgga tatggctatg atgtcccttt tcttatcgat gtattcaata
      361 atatccttaa tactaaagaa gaaaaacgaa tcgcattagt aggatatgga aatcttggca
      421 aagcattgaa acacaacaat tttagaagaa atgaaaatct aaatatcgtg tgcgtctttg
      481 ataatgatcc agcactgatc aatcgagtaa ttgatggaga aatgatctat ccgattgatc
      541 gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc aatctctacg gtaccaagta
      601 aatattcaca atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt
      661 ttgcgcctga cagagtaact gtcccagctt atgtcaatgt tcaatacatt gatttgacaa
      721 ccgagttgca gacgcttatt tactttgatg agaattactc agaagttttt tcttaggaaa
      781 atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag
      841 tactaaatga agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga
      901 atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta
      961 tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat
     1021 ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta
     1081 ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac
     1141 gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata
     1201 taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa
     1261 accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac
     1321 tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca
     1381 aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc
     1441 gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg
     1501 tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg
     1561 cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga
     1621 tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat
     1681 ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat
     1741 cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga
     1801 tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc
     1861 gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct
     1921 acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg
     1981 gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg
     2041 tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg
     2101 aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga
     2161 aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac
     2221 aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc
     2281 gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt
     2341 caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt
     2401 atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg
     2461 gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg
     2521 gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga
     2581 ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg
     2641 ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt
     2701 cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa
     2761 tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt
     2821 atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc
     2881 ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg
     2941 aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct
     3001 tactcgtttg aatgaaacga tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg
     3061 tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga
     3121 aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat
     3181 cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac
     3241 agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc
     3301 tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga
     3361 attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat
     3421 tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag
     3481 ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc
     3541 agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt
     3601 gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga
     3661 agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga
     3721 agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa
     3781 agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca
     3841 gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca
     3901 ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc
     3961 tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag
     4021 ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat
     4081 gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc
     4141 aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga
     4201 agctttatta gaagtggtag ggcatccaga gagaaaagta aaaacgatcc atatcgctgg
     4261 aacaaacgga aaaggttcta ctgtagctta tttgagaagc ctattagaag aggctgggct
     4321 gacagtaggt acatttactt caccttatat cgaaacgttc aatgaacgga tcgcaatcaa
     4381 tggcaaaagc attccagaca atgatttgat ccagcttgta aaaaaatatc agccactgat
     4441 caaacaactt gatcaatttg aagcagtagc tggtattact gaattcgaga cattaacagc
     4501 aatggcattt gattattttt tgatgaagaa agtggacgta gcgattatcg aagttggctt
     4561 aggcggctta ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat
     4621 tggacttgat cataccgaaa tacttggaga tacaattgaa gaaatcgctg cacaaaaagc
     4681 tggaatcatc aaacaaggcg ttccagtagt aacaggaaat attgccccag aagcttttac
     4741 tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta
     4801 taaaataaac tatctgcatc ctgatgagat gtggggagaa gtatttgaat tttttaatga
     4861 aataggaaag atccctcaat taaccactcc gcttttaggg caacatcaag ctgaaaatgc
     4921 aggagtagcc atcgaattgt attttgttta ctgtcaattg gaaaagctgc ctttccgaga
     4981 aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag
     5041 taacgagcca ttagttgttt tagatggtgc ccataatacg catgcaatga accggcttgt
     5101 tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac attttgtttt ccgccttacg
     5161 aactaaaaat gttagagaaa tgctagaacg attgcagcaa gtaccaaatg tccatattta
     5221 tttgacgacc tttgaacatc caaaagccat cgaattaagt gaagtatcag atttagcaag
     5281 tgagagagta tcaatcgttt cattgtggca attcggatta gcagatatat tggaaaagat
     5341 gacaaatgaa gacgtattag ttgtgaccgg ttcactttat ttcatttcag aagttcgcca
     5401 tttattaata caattaggag gagatcatga agtataatgc agtgattttt gacatggatg
     5461 gtttgctgtt cgacacagag ttagtgtatt acgaagccac acaagtagta gcagacaaga
     5521 tgggttttcc ttatgataaa gacctgtatt taaaattctt aggtgtttca gatgaagaag
     5581 tatgggcgaa ttatcatatg attttctcgg aatttggcaa aacaagtgta caaagattca
     5641 tagatgattc ataccaggaa acgcttgaac gtttttcgca aggagcggta caaatgaagc
     5701 ccggtgtgtt ggaactcctc tcttttttag aagaacatca gattcccaaa gttgtcgctt
     5761 ccagtaacca acgagcagtc attgaacttt tattgaagaa gaacaatctg tttgaacgat
     5821 ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa accagaccca gaaattttct
     5881 tgttagctaa tcagaagcta ggtacagcga agaaagaaac attgatttta gaagattcac
     5941 aaaatggtat tttagcagct tctgcggctg agatcccggt agtcatggta ccagatctgc
     6001 ttccaccatc ccaagaattg atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa
     6061 tccctgcatt gttaaaataa aaatccatct cttttcgtat cttattttag ataagataga
     6121 aaggagatgt ttttttggga aaacgattga tcaaagaagt acctactagc tctttaccaa
     6181 gggaacgtat ggaaatttat ggcgcggaag ctttatcgga tcaggagctt ttggcaatct
     6241 tactgcgtac cggtcaacac ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa
     6301 cgtttggagg actggcctct ttacgacagg ctactttgca tgagttagaa gagattcgag
     6361 ggataggacg cgtgaaagca atcgaaatca aagccttgat cgaattaggt cgacgaattc
     6421 agacggatca tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg gcccagcaat
     6481 tgattctgga aatgaaagat cacaaacaag aacatttggt ttgtatttat ttggacacga
     6541 agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc
     6601 atcctagaga aattttccat tatgctgtac gttattgtgc agcaaggatc gttttagcgc
     6661 ataatcatcc ttctggtaat gttttaacca tagtataata aataatagta gttaaagtag
     6721 tttaagagga gatattaaag ggatatttta ctttaaataa aaattaacaa ggtttataat
     6781 ttgtagtagt taaagtattt aaagaggtgg gaacatgtga aggttcaaag gatagaagtg
     6841 gagaataagc cgtatccatt gtatttatta ctagataaag aataccagct aatagaacca
     6901 gtaatgaaat ttattaaata cttagataat actggtaagt ctcctaatac cattaaagca
     6961 tactgctatc atttaaagtt gctgtacgag ttcatggaac agagaggtgt tattcttaat
     7021 gatattaact ttgagttgtt agcagacttc gtaggttggt tgagatatcc ttcagcatca
     7081 aatgtaattg atcttcagtc aaaaaaagcc ataagagaag aaacgacagt gaatacaatt
     7141 ttaaatgtag ttatgagttt tcttgattat ttaagtagat taggagaatt taaatcaatt
     7201 gatgtattta aacaagcaaa gggaagaaat ttcaaaggat ttttacatca tgttaataag
     7261 ggtagatacc aaaagaatgt cttaaagtta agggttaaaa agaaacagat aagaacattg
     7321 agatcagagg aagttaagca aattattgat gcttgtcata cgaaaagaga taaattaatt
     7381 ttaatgctta tgtatgaggg tggtttaaga atcggtgaag tgttatcgct taggcttgaa
     7441 gatattgtca cttgggacaa tcaaatccat ttaacaccta gagatgttaa tgttaatgaa
     7501 gcttatatta aattaaggaa ggaaagaaca atacatgtga gtaaagaact tatgtcactt
     7561 tatacagatt acttgatata tgagtatagt gaggaattgg agcatgatta tgtttttatt
     7621 tccttaaaag aaggctattt tgggaaacca ctaaagtacc aaagtgttct tgatctagtt
     7681 agaagaatag ttaaaaggac tggaatagaa tttacatcac atatgcttcg ccacactcac
     7741 gcaacgcagc taattaggga aggatgggat gttgcgttcg ttcaaaagag attaggtcac
     7801 gcacatgttc agacaacgtt aaatacctat gttcatcttt cagatcagga tatgaaaaat
     7861 gagtttaata aatacctcga gagaaaggag cataagaaat gaatgcttct agtaaaagga
     7921 aaattattag tcagagtgag attagcaaaa aaatagctgt aatgaatgaa gaaatgcagg
     7981 ggttttgggc taataatagt tgggatataa gaaaatgtcc acatccttct gccatagaat
     8041 taagtaagaa tcctgcttta aggaatcgtt gggttcgttt tgaacgtgtt aaaaatctgt
     8101 ggttaagaac agaattgaaa tatttttatt tttaccattt aaacaatgga atatggaatg
     8161 caaaaactgt ctggattaga aaaggaacag taattaataa aatgttagat ttcttagatt
     8221 taaagtatcc tagcattact tcaattactg aagttcctat tgaaaaagca atgacggagt
     8281 atagaactta tttgacaaaa cggggtgtta gaattaccac tactaattat aagattactg
     8341 ctaatcaaga aaaaacacct gtaaaagcta attcctacta tgttactaat ctaaaacaat
     8401 ttatggagtt ttatgagaac ttttattttg atggagagga gtgggataaa gacgtttggg
     8461 atagacgtaa cttacctttg ccagatgata aggttaaccc aacacaatat gaatatacaa
     8521 ttaactttaa agggtttcgg aatacatatt ttaaacaact tgtaaaaaga tattgtaagt
     8581 tgagattgaa catggatagc ttttcctatg taagtgatat tgcccaaaga cttaaagagt
     8641 tctttaattt tctggacatg aaatttaaac aagttcagag agtacaccaa ttaacgagag
     8701 tggaaattga agcatattta agtgaactaa acatgatggg aataaaacct agtacaataa
     8761 ctgggaggat ctctatattg gaaggactat ttagtaccct tcttaggcta gaatgggatg
     8821 atgttccttc caaaatatta atttattctg aggactatcc gaaaatacca agagcaaaac
     8881 cacgctttat agatgaattc gtcctagagc aattgaacag tcatcttgat aaattacccg
     8941 aatatatagc tacgatgact atgattgttc aagaatgtgg aatgaggata agtgaattgt
     9001 gcaccttgaa aaaaggctgt ctattagagg acaaagatgg agatttcttt ttaaagtatt
     9061 atcaatggaa aatgaaaaag gagcatatag ttccaatatc taaagaggta gctttactta
     9121 ttaaagttcg ggaagataaa gtttcagagg aatttccaga tagtgaatac ctctttccaa
     9181 gaaaagatgg atcgccatta aaacaagaaa catttagagg tgagttaaat aaattagctt
     9241 atgagcaaaa tatagtggat aaatcaggtg agatttatag attccatgcc catgcctttc
     9301 gccatacagt aggaacaaga atgattaaca acgggatgcc ccagcatatt gtgcagaaat
     9361 ttttggggca tgaaagccca gaaatgacaa gcagatacgc tcatatcttt gatgaaactc
     9421 taaaaaatga atttactaaa tttcaggaaa aactggttac caataatgga gatgtgcttg
     9481 atctagatga agataatgaa gtcgatgatg tagagcttca atggttcaag aaaaatataa
     9541 atgcacaagt gcttccaaat ggttattgta gattgccagt agtagcaggt ggttgtccac
     9601 atgcgaatgc atgcttagat tgcactcact tctgtaccag taagcaattc ttaccacagc
     9661 acgaagaaca gttagagcgt acagaagagt tattagccat agctaaggat aaacaatggc
     9721 aaagacaagt agagactaat agccgtgtta aagagcgttt agaacaaatc attggaagtt
     9781 tgacggggta attatcaatg gataaacaag ttagaaatac aacagaaatt gtacgtttgg
     9841 cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa agcgatttct aaattttcga
     9901 ttgaaggtaa agttattaat tttaattcaa tagcaaagga agctaatgtt tctaaatcat
     9961 ggctttataa ggaacacgat attaggcaaa gaatcgaatc ccttcgtgag cgtcaaataa
    10021 cagcaaatgt agtctcaaaa cccaagaaaa gttctcgttc ggaggaaatc cttattaaaa
    10081 ccttaaaaag aagagtaatg gaattagaaa aagaaaataa aaaattacag aaccaaattc
    10141 aaaaattata tggagatctg tataataaag aataattatt aatctgtaga caaattgtga
    10201 aaggatgtac ttaaacgcta acggtcagct ttattgaaca gtcatttagg tatatgtcca
    10261 atctagggta agtaaattga gtatcaatat aaactttata tgaacataat cagttacgag
    10321 gtgaaatcat gagcaatttg attaacggaa aaataccaaa tcaagcgatt caaacattaa
    10381 aaatcgtaaa agatttattt ggaagttcaa tagttggagt atatctattt ggttcagcag
    10441 taaatggtgg tttacgcatt aacagcgatg tagatgttct agtcgtcgtg aatcatagtt
    10501 tacctcaatt aactcgaaaa aaactaacag aaagactaat gactatatca ggaaagattg
    10561 gaaatacgga ttctgttaga ccacttgaag ttacggttat aaataggagt gaagttgtcc
    10621 cttggcaata tcctccaaaa agagaattta tatacggtga gtggctcagg ggtgaatttg
    10681 agaatggaca aattcaggaa ccaagctatg atcctgattt ggctattgtt ttagcacaag
    10741 caagaaagaa tagtatttct ctatttggtc ctgattcttc aagtatactt gtctccgtac
    10801 ctttgacaga tattcgaaga gcaattaagg attctttgcc agaactaatt gaggggataa
    10861 aaggtgatga gcgtaatgta attttaaccc tagctcgaat gtggcaaaca gtgactactg
    10921 gtgaaattac ctcgaaagat gtcgctgcag aatgggctat acctctttta cctaaagagc
    10981 atgtaacttt actggatata gctagaaaag gctatcgggg agagtgtgat gataagtggg
    11041 aaggactata ttcaaaggtg aaagcactcg ttaagtatat gaaaaattct atagaaactt
    11101 ctctcaatta ggctaatttt attgcaataa caggtgctta cttttaaaac tactgattta
    11161 ttgataaata ttgaacaatt tttgggaaga ataaagcgtc ctcttgtgaa attagagaac
    11221 gctttattac tttaatttag tgaaacaatt tgtaactatt gaaaatagaa agaaattgtt
    11281 ccttcgatag tttattaata ttagtgacat ttgcatgctt caaagcctgt cggaattggt
    11341 ttttagtgaa aagaacacga tattcacggt ttacccactt ataaacaaaa gatcgatact
    11401 ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc aagaacaatc aatacagagt
    11461 ctacacttgg cttaggatga aaatatagtg gtggtacttt tttgagcatt tttatatcca
    11521 tctccaccat taatagtaaa cccaaagctc gttgcagatt ttgcaatctt ttcgcaaatc
    11581 ccttctcaac gataagatag ctatatttag cctgactttc aaaggtaatt cttttgacaa
    11641 tatccgtact gatgttataa ggaatattac catatatctt atagtttata tgttttggga
    11701 aggaaaattt tagaatatcc gtttgaatca cttttatatt ctcagagggg tttaccgctt
    11761 ctttagtcac ttgacataag cctccatcaa tttctatagc agtaactgat cgactcattt
    11821 tgactagctc tttggtaaaa tgtccttttc ctgatccgat ttctattacg ttgtcttgtt
    11881 tactgatatt cgtgtgattc aatatttctt ttacatgctt tttagaagta ataaaatttt
    11941 gcgtgtcttt agggtttttc tggttcatta taaccttctc cttactggtt ataatgaact
    12001 gacattaagc gttcattata accaattaat tttgatttgg ttgataacga actttattaa
    12061 taacaaatat agaaaaagta cccatacttt tatctccttt ccattttttt cattatgaat
    12121 gagataaagt aatttctact actgcgatac tggtgcacat aattgaaacc tccttatgta
    12181 actgaatatg attgtatcat cttttcattt tagaataaac ctttatatgt aattttgcac
    12241 ttgacacttc agatggacgt tttcttcaaa ataatatgtt ttgaaacttg ataagagaag
    12301 ccactgtctt tactaaaaag taaaggctgc gataaaggac agtgacttta ataaagttgc
    12361 actgttgtat tgtctttgga taaaattgtc taaagggaag ttttatcccc aacgaaaaaa
    12421 catctaaagt ggtaagtaat gaaagaatgt cgataaagta ggaatggggg atctttttga
    12481 atacaataga atcacagttt gataaggttg cagaagatta cgatttcgtg aatgagcttt
    12541 tgaatgatta ttcattcttt gtgtctaata tgtctccaaa gaaaggcaga gcattagata
    12601 tcggatgtgg ctcgggtttg ttagtggaga aattagcaag ttattatgat gaagtggtag
    12661 ggattgatat ttctaatcaa atgctcgatc ttgccaaatc taaacgtcaa ctaacaaata
    12721 cggtctatct gaatatgaat gcagaacaac ttaattttaa tgagaagttt gattttattg
    12781 taagccgaac aacctttcat catttggatg atatagccag cgtaatacag caaatgaagg
    12841 aactgttgaa tgaggaagga agaatagtta ttcttgataa tgtatctgaa gtcgagacac
    12901 cgcctaccta tgtttataaa ttaggggcaa tccaagagtt tttaccgcat tgtttcaaat
    12961 ttggaataaa aaatgcgatt agaatttata atcacaacac atctaaatca tggcttgagc
    13021 atttagcttc agataagtac ctatctgaac aaaattatta cgatttatat gaaaagttgt
    13081 tgcctggatg ccaattccat aaaatgggtt gggcaatggg ggttgtctgg acaaaataac
    13141 ttaaagctgt ggctttgaaa agttgaattt gtgattgttt tcagagctat cgggtgcttt
    13201 agtataaata acaatgaata aaaaatcagc ctgtatctta tgttactatt aagtaattta
    13261 agatacaggt tttactattt ttgaaataag taccttccac ttcaaataca tgtattcaaa
    13321 gtgaaagtat tgatttaaat gattcttttg taatttactc ttgataatat gaaattccat
    13381 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
    13441 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
    13501 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
    13561 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
    13621 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
    13681 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
    13741 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
    13801 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
    13861 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
    13921 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
    13981 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
    14041 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
    14101 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
    14161 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
    14221 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
    14281 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
    14341 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
    14401 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
    14461 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
    14521 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
    14581 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
    14641 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
    14701 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
    14761 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
    14821 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
    14881 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
    14941 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
    15001 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
    15061 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
    15121 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
    15181 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
    15241 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
    15301 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
    15361 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
    15421 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
    15481 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
    15541 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
    15601 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
    15661 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
    15721 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
    15781 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
    15841 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
    15901 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
    15961 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
    16021 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
    16081 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
    16141 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
    16201 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
    16261 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
    16321 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
    16381 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
    16441 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
    16501 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
    16561 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
    16621 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
    16681 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    16741 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    16801 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    16861 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    16921 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    16981 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    17041 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    17101 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    17161 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    17221 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    17281 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    17341 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    17401 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    17461 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    17521 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    17581 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    17641 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    17701 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    17761 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    17821 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    17881 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    17941 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    18001 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    18061 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    18121 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    18181 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    18241 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    18301 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    18361 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    18421 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    18481 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    18541 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    18601 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    18661 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    18721 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    18781 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    18841 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    18901 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    18961 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    19021 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    19081 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    19141 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    19201 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    19261 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    19321 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    19381 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    19441 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    19501 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    19561 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    19621 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    19681 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    19741 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    19801 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    19861 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    19921 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    19981 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    20041 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    20101 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    20161 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    20221 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    20281 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    20341 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    20401 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    20461 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    20521 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    20581 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    20641 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    20701 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    20761 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    20821 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    20881 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    20941 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    21001 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    21061 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    21121 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    21181 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    21241 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    21301 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    21361 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    21421 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    21481 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    21541 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    21601 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    21661 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    21721 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    21781 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    21841 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    21901 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    21961 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    22021 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    22081 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    22141 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    22201 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    22261 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    22321 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    22381 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    22441 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    22501 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    22561 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    22621 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    22681 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    22741 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    22801 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    22861 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    22921 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    22981 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    23041 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    23101 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    23161 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    23221 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    23281 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    23341 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    23401 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    23461 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    23521 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    23581 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    23641 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    23701 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    23761 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    23821 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    23881 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    23941 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    24001 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    24061 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    24121 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    24181 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    24241 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    24301 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    24361 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    24421 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    24481 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    24541 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    24601 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    24661 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    24721 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    24781 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    24841 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    24901 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    24961 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    25021 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    25081 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    25141 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    25201 accaataaaa ttttattttt ttaa
//
LOCUS       DAZUMB010000031        25212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000031 DAZUMB010000000
VERSION     DAZUMB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.031"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG80L_001055"
     tRNA            12..84
                     /locus_tag="ACG80L_001055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG80L_001056"
     tRNA            88..160
                     /locus_tag="ACG80L_001056"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG80L_001057"
     tRNA            168..249
                     /locus_tag="ACG80L_001057"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG80L_001058"
     tRNA            267..339
                     /locus_tag="ACG80L_001058"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG80L_001059"
     tRNA            351..422
                     /locus_tag="ACG80L_001059"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG80L_001060"
     tRNA            439..524
                     /locus_tag="ACG80L_001060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG80L_001061"
     tRNA            535..608
                     /locus_tag="ACG80L_001061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG80L_001062"
     tRNA            618..691
                     /locus_tag="ACG80L_001062"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG80L_001063"
     tRNA            711..783
                     /locus_tag="ACG80L_001063"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG80L_001064"
     tRNA            811..884
                     /locus_tag="ACG80L_001064"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG80L_001065"
     tRNA            898..971
                     /locus_tag="ACG80L_001065"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG80L_001066"
     tRNA            995..1084
                     /locus_tag="ACG80L_001066"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG80L_001067"
     tRNA            1097..1170
                     /locus_tag="ACG80L_001067"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG80L_001068"
     tRNA            1189..1264
                     /locus_tag="ACG80L_001068"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG80L_001069"
     tRNA            1266..1336
                     /locus_tag="ACG80L_001069"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG80L_001070"
     tRNA            1350..1423
                     /locus_tag="ACG80L_001070"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG80L_001071"
     tRNA            1448..1535
                     /locus_tag="ACG80L_001071"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG80L_001072"
     tRNA            1557..1628
                     /locus_tag="ACG80L_001072"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG80L_001073"
     tRNA            1651..1740
                     /locus_tag="ACG80L_001073"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG80L_001074"
     tRNA            1753..1826
                     /locus_tag="ACG80L_001074"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG80L_001075"
     tRNA            1845..1917
                     /locus_tag="ACG80L_001075"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG80L_001076"
     tRNA            1921..1993
                     /locus_tag="ACG80L_001076"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG80L_001077"
     tRNA            1998..2068
                     /locus_tag="ACG80L_001077"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG80L_001078"
     tRNA            2081..2154
                     /locus_tag="ACG80L_001078"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG80L_001079"
     tRNA            2166..2237
                     /locus_tag="ACG80L_001079"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG80L_001080"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG80L_001080"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS5023322.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG80L_001081"
     CDS             3826..5298
                     /locus_tag="ACG80L_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5023323.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG80L_001082"
     CDS             5381..6169
                     /locus_tag="ACG80L_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5023324.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG80L_001083"
     CDS             complement(6236..6904)
                     /locus_tag="ACG80L_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS5023325.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG80L_001084"
     CDS             7019..8731
                     /locus_tag="ACG80L_001084"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023326.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ACG80L_001085"
     CDS             8734..10503
                     /locus_tag="ACG80L_001085"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023327.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ACG80L_001086"
     CDS             complement(10708..11274)
                     /locus_tag="ACG80L_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5023328.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ACG80L_001087"
     CDS             complement(11274..12230)
                     /locus_tag="ACG80L_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS5023329.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ACG80L_001088"
     CDS             complement(12230..12886)
                     /locus_tag="ACG80L_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5023330.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="ACG80L_001089"
     CDS             13256..15274
                     /locus_tag="ACG80L_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5023331.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /gene="leuS"
                     /locus_tag="ACG80L_001090"
     CDS             15701..18115
                     /gene="leuS"
                     /locus_tag="ACG80L_001090"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS5023332.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="ACG80L_001091"
     CDS             18463..20016
                     /locus_tag="ACG80L_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS5023333.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="ACG80L_001092"
     CDS             complement(20397..22181)
                     /locus_tag="ACG80L_001092"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023334.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="ACG80L_001093"
     CDS             complement(22196..23917)
                     /locus_tag="ACG80L_001093"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023335.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="ACG80L_001094"
     CDS             24017..24778
                     /locus_tag="ACG80L_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5023336.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7801 a         4631 c         5365 g         7415 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tc
//
LOCUS       DAZUMB010000032        24951 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000032 DAZUMB010000000
VERSION     DAZUMB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.032"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..547
                     /locus_tag="ACG80L_001095"
     CDS             191..547
                     /locus_tag="ACG80L_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5023337.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            557..1456
                     /locus_tag="ACG80L_001096"
     CDS             557..1456
                     /locus_tag="ACG80L_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5023338.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1853..2932)
                     /locus_tag="ACG80L_001097"
     CDS             complement(1853..2932)
                     /locus_tag="ACG80L_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5023339.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            3168..3758
                     /locus_tag="ACG80L_001098"
     CDS             3168..3758
                     /locus_tag="ACG80L_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5023340.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(3886..5235)
                     /locus_tag="ACG80L_001099"
     CDS             complement(3886..5235)
                     /locus_tag="ACG80L_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5023341.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(5403..6143)
                     /locus_tag="ACG80L_001100"
     CDS             complement(5403..6143)
                     /locus_tag="ACG80L_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5023342.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(6156..7748)
                     /locus_tag="ACG80L_001101"
     CDS             complement(6156..7748)
                     /locus_tag="ACG80L_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5023343.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     regulatory      complement(7886..8060)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(8316..8612)
                     /locus_tag="ACG80L_001102"
     CDS             complement(8316..8612)
                     /locus_tag="ACG80L_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023344.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            8788..9513
                     /locus_tag="ACG80L_001103"
     CDS             8788..9513
                     /locus_tag="ACG80L_001103"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS5023345.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            9506..10348
                     /locus_tag="ACG80L_001104"
     CDS             9506..10348
                     /locus_tag="ACG80L_001104"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS5023346.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            10351..10872
                     /locus_tag="ACG80L_001105"
     CDS             10351..10872
                     /locus_tag="ACG80L_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS5023347.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(11125..11688)
                     /gene="def"
                     /locus_tag="ACG80L_001106"
     CDS             complement(11125..11688)
                     /gene="def"
                     /locus_tag="ACG80L_001106"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5023348.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            11939..12208
                     /gene="rpsO"
                     /locus_tag="ACG80L_001107"
     CDS             11939..12208
                     /gene="rpsO"
                     /locus_tag="ACG80L_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS5023349.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            12396..14522
                     /gene="pnp"
                     /locus_tag="ACG80L_001108"
     CDS             12396..14522
                     /gene="pnp"
                     /locus_tag="ACG80L_001108"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS5023350.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            15032..15298
                     /locus_tag="ACG80L_001109"
     CDS             15032..15298
                     /locus_tag="ACG80L_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5023351.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            15442..17433
                     /locus_tag="ACG80L_001110"
     CDS             15442..17433
                     /locus_tag="ACG80L_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS5023352.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            17787..19088
                     /locus_tag="ACG80L_001111"
     CDS             17787..19088
                     /locus_tag="ACG80L_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5023353.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(19218..20588)
                     /locus_tag="ACG80L_001112"
     CDS             complement(19218..20588)
                     /locus_tag="ACG80L_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS5023354.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(20770..21093)
                     /locus_tag="ACG80L_001113"
     CDS             complement(20770..21093)
                     /locus_tag="ACG80L_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS5023355.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(21418..22026)
                     /locus_tag="ACG80L_001114"
     CDS             complement(21418..22026)
                     /locus_tag="ACG80L_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS5023356.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            22356..23072
                     /locus_tag="ACG80L_001115"
     CDS             22356..23072
                     /locus_tag="ACG80L_001115"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS5023357.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            23085..23780
                     /locus_tag="ACG80L_001116"
     CDS             23085..23780
                     /locus_tag="ACG80L_001116"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS5023358.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            23864..24493
                     /gene="udk"
                     /locus_tag="ACG80L_001117"
     CDS             23864..24493
                     /gene="udk"
                     /locus_tag="ACG80L_001117"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS5023359.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT         7818 a         4824 c         5167 g         7142 t
ORIGIN      
        1 caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag ggaaagttgt
       61 ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat aaattgagaa
      121 cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc tatgacatga
      181 ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga agaataaatt
      241 agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat ttgcagaaag
      301 ccaagtcagt tgccaattgg atacggcatt tcgattgatc attgtcaaaa aagatacaca
      361 aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa
      421 agacagtgca atactttaca tggatgatcc atccacactt gctttagaac caacttataa
      481 tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa
      541 agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg tggacatcaa
      601 taaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac
      661 tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag ctgttgtcga
      721 atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg agttggcaga
      781 tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa atgaacggta
      841 tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg actatcagtt
      901 ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg
      961 gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa
     1021 aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca ctcatttcgt
     1081 tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgttgga acaaattaaa
     1141 tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa atatcaaaca
     1201 agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc acacgattta
     1261 cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt ttgtcttgaa
     1321 agaacagttg ctagacagct atgatcctat gaaattacgt gtcctcttgc gtgcccatca
     1381 cccagctttt caggcctatc aaacacaaca tttgcaagag gaatttgaac aagcgatgac
     1441 aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata
     1501 aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt tgcggaaaat
     1561 aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta gatgcaagcg
     1621 tgtattcttt ttttgaacga taatcacaaa aaaaaatata atcgtaaatt tcaagtttaa
     1681 gttagccacc catggtgacc cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg
     1741 agtgggccct tgacaatgag ctgaggcatg gtagtctccc gactggctgg ccccggccag
     1801 ctttccagac acaagccatg ccgagggagg ctcgttgtca agggcccacg tgtcaagccg
     1861 tcaaccgttc ctgcctaaat gcctggacga acgtgtcatg cggcgttgca tagcccaaaa
     1921 tctttcgcgg gtaatcgttc atccaacgct gaatccgcaa gatttggttt tcgctcacgt
     1981 tgccgattgg gctgcccttg gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg
     2041 tcccgcgctc ccaggaagca taggggtggc tgaagtagat gtttgccaca tcctgcaagg
     2101 catcgtgcag tcccgcgaac tccgagccgt tgtccgaggt aaccgtcttg aacagctggc
     2161 cgaagtacgc accagcccgt tctctcagct gtcggaccgc ttggttgacc gattcggcat
     2221 ccttcccggc cagcttcaga atgacctcga agcgtgtctg ccgttccacc aacgtcagca
     2281 gcacggcatc cgacttggtc ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg
     2341 ttttccgggt accaaccgcc tccgggcgct cttcaatgga tttccctagc acccgtttat
     2401 tcgggcggtt gccatgacgg gacgatttcg gtttgcggga cagtttttct agcaggtcca
     2461 tattcttcgt tttcatgatg ccacggtcga tccactgata gagggtggtg gtcccaggga
     2521 taatggccgg gtcgaacaat tcgtgttttc tggcaaagcc caccaccacg tccggggacc
     2581 atttttccaa cagcatcatg tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata
     2641 agggccgtct gccgcagttc aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt
     2701 cgtagacgtc atgataatag tcgtagacct tcccgttctg attctggcgg ttaagctgag
     2761 taacggtccc gcgtttgatt tcgttgttta tggtctgcgg cacgcagccc aggacatccg
     2821 caacctgacg gttggaatag ttttcctttt tcaggatggc gatttggcaa cgttctgagt
     2881 aggttaggtg ttttcccttg cgtgaaactg tgttatcatg gttttgcgtc atgtgaattc
     2941 atccttgtct attgagagtt gtggtaactt caatataaca tggaattcac gtggcgtttt
     3001 tttatttcca aatggctaag ttaattataa aatccgcctt tttttaaaag taacaaaatt
     3061 tcgaaaaata tgcaacaatt ttcagaaaat tccctatata aaacgatggt tatttaataa
     3121 cgtaagtgat aaaataaaag cgctgaatca tctgaggagg aatcatcgtg acaaagtcga
     3181 gattagaagc atttactgat ggagtggtag ccattgtttt gagagttatt gtattagaga
     3241 tcaagatccc taatacatct agctttcatt ctttatgggt catccgaaat acattattag
     3301 cttataccat cagctttata tttgttgcgg tgatctgggt gaaccatcat cgtatatttc
     3361 agatggcaga acgaattaat taccgagtgg tctggtcgaa tatctttttg ctcttttggc
     3421 tgactttatg tccttctgtt actagttggg tcgggcgtaa ccctgaagct ttttggcctg
     3481 agatggctta tgtcttagta tacacgatgt ggagtttttc ttttggtatc ttgatgaggc
     3541 aaatcatgaa agcaaataaa ccagattccc atgtcgtgag agttttgtca aaggaccgtc
     3601 gaagtcttat ctccatgatg attaacttat gcttgattgc cggtgtcttt tttgtaccgt
     3661 tgatcgggtt attcggacgt ttttttgtat ctggtatttg gattttttcc tataaaaaag
     3721 cagatgccta ttatcagcgg ttgtttccgg ggaaatagaa aagaggctgg gacagaagct
     3781 tttatctcca agaaataagc cgaaatttca caaaaattta agaagcaatt ttcgtaaatt
     3841 ccttcttatt tctcgaagct gaccacttct gtcacaacta ctttcttact tcttacgttc
     3901 atatttcata tgatagatta gtaatagaag caagaaccac acaggtgtta gtaataaagc
     3961 gattctcgta tcttttccta ggaacaagac gacacacaca aaggccatgt aaagaaatac
     4021 cagataatta ctaaatgggt acaacggcat tttaaatgaa tgagcttttt tgccgaggat
     4081 acttttacga tatttcatgt gcgtgtagac aagcatactc cagataaaca agaaacaagt
     4141 agtggcaata ctgcttacta gtgtgaatac ttcactaggc atcacgtaat tcaagataac
     4201 agaaatgaaa atgacgatag tagaaaatgt caaagcattc cctggcacat gatgtgttgt
     4261 taactttttg aattttttag gggcactgcc ttcttgcgct aaagaacgaa gcatacgtcc
     4321 tgtactatat attgcactgt tgcaggcaga agctgctgca cttaatacga ctaaattgat
     4381 caaggaagca gcaatcgtga tcccaatttc cgaaaatact tctacaaatg gactgttatc
     4441 tggattcaag ctgttccatg gatagattgc cataatgaca aaaagagatc ctaagtaaaa
     4501 taagatgatc ctgataggaa tattattgat agcttccggc aacacttttt caggattttc
     4561 tgtttctcca gcgactaatc caactaattc tatcccagca aaagcaaagg ttaccatttg
     4621 gaaagctaag ataaatcctt ttgcaccagt tggaaagaat ccgccatgag accatagatt
     4681 cgtgatactg gctgtgccaa tatctgtcgt atagtgggtg aggatcatat aagctcctac
     4741 gaggataaag gcaatgatcg ctacaacttt gatcaatgca aaccaaaact ctaactcacc
     4801 aaacaatgag acagccacca tattcaagca aagaagaaga cctaatgcaa tcagttcagg
     4861 aagccactga ggaacaccag gcgcccagta gcgtacatac attccgatgg ctgttaaatc
     4921 ggccattgca atcgataccc agcagaacca atacgtccag ccagtgatga aagccgcttt
     4981 tttgccaaga tattctgcaa caaaatccag aaaagaatgg ttattggtgt ttgataacag
     5041 aagttcgcct aacgcacgca taatgaaaaa acagatgcat ccagtaataa gataggcgag
     5101 caatatcgaa gggccagcta gctggataga ctttccggaa cctaaaaaca accccgttcc
     5161 aatcgctccg ccaatagaaa tcagttggac atgtctattt ttcagtccac gctgcagttc
     5221 ttgctcttca ttcattttta aatcatccta actaaaaaag tttaattact tccatattaa
     5281 gagagttttc tgaaaaacac aactaagatt ccaaaatttt cagaaattta ccttttatga
     5341 gtaattcata aaaaagaact gcggtaaaca ccgcagctct ttgtcccaat cagctattga
     5401 tcctaagcat ttaaaatacg tgaaaggaat tcttttgtac gttcttcttt cggatggaca
     5461 aagatatctt ctggtgtacc ttcttctgca atcacacctt tatccataaa aatgacacgg
     5521 tcagatacat cttttgcaaa ttccatctca tgcgtcacga taagcattgt caagccagta
     5581 tgtgctaagt ctttgatcgt attaagcact tcgccaacca tttcagggtc taacgccgaa
     5641 gtaggttcgt cgaatagcaa tacatctgga ttcattgata atgcccgagc aattgcgaca
     5701 cgctgtttct gtccaccaga taattgtgct ggtttggctt cgatataacg atccatacca
     5761 actttttcca gattttcgat tgctactttt ttggcagttt ctttatctcg tttcaggaca
     5821 gttgtttgac cagacataca gttttccaag acattcatat tgttgaacag gttgaaggat
     5881 tggaaaacca tacctagatt cgttcgatat ttaggtaaat tatatttagg agctaataca
     5941 ttttctccat tataaatgat ctcgccgcct gttggttttt ccagtaaatt gatacagcgc
     6001 aagaaagtag atttaccaga accggaagaa ccgatgatcg tcacaacttc acctttattg
     6061 actgttacgc tgatatcttt caatacttca ttttcaccga agctttttct tagatgtttg
     6121 atttcaataa ttttttcatt catgattctt cctccttatt cctgcaaatt tgaattgtcg
     6181 atttcttcaa gcttggtata agctgaagga ccatcgattt tcttttctac cgcccgcagg
     6241 attctggtga tcgtaaaggt catgactaag tagatgatcc cgacgatcgt aaatgtttgg
     6301 aagaattgga aattcgcacc ggaagcagag tttccttgga agaacaaatc agctacgccg
     6361 attacactta aaactgctgt atctttgata ttgataacaa actcatttcc tgttgctggc
     6421 aagatattcc gtaaaacttg tggaataaca acttttctca tcgtttggcc atgggtcata
     6481 ccaatggctt gcgcagcttc gaactgccct tcatctactg caaaaatccc cccacgaacg
     6541 atctctgtca tgtaagctcc agtattgaga gagacaataa acaaagcggc aatcgttcgg
     6601 ttcaatgaaa tgccaaaagc gagtgccaga ccgtagaaga tgaccattgc ttgtaccatc
     6661 atcggtgttc cgcggaatac ttcgacatag atagaaagaa gtgcatttcc gattttttgg
     6721 aagaagcgag tcagtgcatt tcctgattca ggtaccgaac ggaagacacc aatcagtaat
     6781 ccaagtgtag taccgacaac tgtcccgatc aatgcgatga acaaggtaag tccagcacct
     6841 cgtaagaaca tattgccgta ttgtttccaa atcgtagtga aatcagaaat gatgccaggt
     6901 ttttcttcac tatcagttgc agcaggctga tctttgactg cttggtccat aattttggtt
     6961 cgttcatcgt tagagatgcc agaaagaatc tggtttactt tttggatatc cgggtcgcct
     7021 ttacgcatac caacagaaat ctgaacatct tccgggtttg tctggaagcc atctgcaccg
     7081 ctgaattcaa gcatttccaa gtctttgttc acactagttg ccgttacacc ttccggacgt
     7141 tcgctgacat aaccatcgat cattccagaa gctaaagctg ttctcattgc tgagaaatta
     7201 tccattgctt gttgtttctg cacatctggt atctgatcga tcacagtata gtggaatgta
     7261 tttaactgag cagtgatctt tgctccagaa agatctgcta aactttttgc ttttgcatat
     7321 ttgctatctt tttgaactac tactactaat tgtgattcgt aataaggatc ggtaaaatca
     7381 atttcttttc gtcgttcagc agtagggctc attccggcga tgatcgcgtc gatcttgcct
     7441 gattgaaggg caggagccag accatcccat ttcgtttgga cgatgactaa ttttttccct
     7501 aaaccatctg ctactttttt agcgatttga acatcataac cgccggcgta actattttgg
     7561 ccttgaattg gcaaggcatt attctcatct gtttgttgag accagttgaa gggtgcatac
     7621 ccagcctcca tacctacgcg aaactcatcg ctcggtgttt tttcatctgc atttgccaga
     7681 gatcccacac tgaatagtga gaagacaaaa gcaaatagta cggcaactga aaccatattt
     7741 tttttcattt ttttctccct ttctgattat atgatacacg agcggtctag atgaaatgcg
     7801 gtaaagcttt atctgaaaaa ataaaaagcg accgatttat agatataaaa aggccgctaa
     7861 aattccataa ggtgataaac ctctcacaaa acatagcgca acttagcatc actgctaaga
     7921 cagtccgcaa gctcttaact tgcgtcccaa catacactcc aagcaaaaaa gtgcacattt
     7981 cggcgatcct ccctattcct gcttctccat gtttgcttca ctccacaggg ttaatgattc
     8041 tcgcgacctc tacctcattg attgaggcat ctcgtattca gttatttata caaaaaaagt
     8101 ttacgatact cgagaggctt tgtcaatcta taactgctgg tttttgaaaa aatactgata
     8161 aatatagaaa tctttacttt taccgaaaaa acaatcaaaa tgtgaatcat ctatccattg
     8221 caaacgatag tataattata cacaaaaatg tatgtaatac aataaaaaaa gaactagaac
     8281 agaaaatttc ctgtgtccag ttccatctcg atcatttaag ctgccgcttt ctttggctca
     8341 cgtcctagga tcgcttgcaa aagaacaacg acgccataaa tggctaatat tagataaatc
     8401 aaacttgagt attgggtgat tccccagccc catgttcgat atacggctaa tgtaatggct
     8461 aaaacaattg cggcaatcac gttgaataaa gcaaaagccc aaagatttcc attttctttg
     8521 cgtacaagat aaaaaacaga gaaataaacg cttaacgcta accaggcgat acaagccaga
     8581 ataacgcccc aataaataaa gaatggtaac atatcgattc acctcacttt tatacagcag
     8641 tattgtaaca cgttttcatt cttttgtcag tgaacattgt gataaattgc tcaaatgccc
     8701 ataacacttt tttattccca tttttctgac ttttcaacaa taaatttgtt cgagtatggg
     8761 ctataatgga aagtgaggtg acaaaacatg aaacaattaa cagtagacga agcagcaaaa
     8821 gagatattag cttatcagca aattacattt ttgaccggtg caggggtatc cacaccttct
     8881 ggagtgccag actatcgatc tttgtcagga atctaccacg ggatggagca gccggaatac
     8941 ttgctgagtc atcaagcaat ggtgaatgaa ccggataaat tctatcaatt tgtcaaaaag
     9001 atttatcatc ctgaagcaaa acccaatatc attcatttgg aaatagcaca tctagcgaaa
     9061 gaaaaaaatg tttgggttgt ttcccaaaac atcgacggtt tgcacgcaaa ggctaaaagc
     9121 ccgcaactcg tcaattttca tgggagtctg tatcaatgtc attgtcgaaa atgtcaacaa
     9181 acggtagact ggaaagaata tcttcacagc gacaaacatc aagcatgcgg aggacaaata
     9241 cgcccagaca tcgtgttata tggtgaaggg tttcaagatg gagtcatgga gcaggcggct
     9301 tatgccgtca gccaagcaga gctgatcgtc attgttggaa cgagttttca agttcatccg
     9361 ttttgtgatc tggtacagtt tcgccagccg tcagccaaaa tcttagtaat caatcaaacg
     9421 cctgtttatt tagcggagcc ttatacgttt attcaagaag acggagcggt tgttttcaaa
     9481 aaaatacaag aattaggagc agattatgac tgaattcaaa accgaaaaag aaaaaatgat
     9541 tgcaggcgag ctttattttg ccaatgatcc tgagctggtt gccgaccgta tgtttgcaag
     9601 aagtcaaagc caaatcatca accaagcaga atcagcggaa ctgcgatccc aattgttgaa
     9661 agagactttt ggccgtacag ggaaaaaaat ctatatggag ccagttatca attttgatta
     9721 cggatataat atatttgttg gggagaattt ttatgcaaat ttcaattgta catttttaga
     9781 tgtcagcacg attgaaatcg gtgataactg catgttcgct ccgaatgtcc agctttacac
     9841 ggcgacgcat ccactacatc cagtgaaacg taacagcggt ctggagtacg caaaacccat
     9901 taaaatcggt aataatgttt ggttaggcgg aggagttatc gtcacgccgg gtgtcactct
     9961 aggggacaac gtagtagtcg gcgcaggttc ggttgtgacc aaatcttttc ccgacaatgt
    10021 agtgattgca ggaaatcctg ctcgtatcat caagacagta gaggaagaac taacagaaga
    10081 gtctcttgaa acattacgtc ataagatcga tatgatcgat cgagagttgg ttgctttatt
    10141 agaaaaaaga atggatacgg taactaaaat aggacaagtg aaaaaagaag aaaaccaagc
    10201 agtttatgat gcaaaaagag agcagcaagt attggataaa gttgttagtc tgctgaaaaa
    10261 taagggatat aaggaaacaa taacagacac gtatgttgat ttgatgaaac actcgcgcga
    10321 ataccaaaac aaaatgaagg aggaataagt gtgatcgaag gagagacacg gcgaaaacaa
    10381 atcatgcaaa ccttggcaaa tacagaaaaa ccagtgagtg ctagcaagtt tgcgaaaact
    10441 tttggagtca gccgtcagat cattgtaggc gatattgctt tactacgagc tgcaggtaaa
    10501 agtatcgtgg caacagcaag aggttatatc ctcgaaacag aagaaaccaa tactgggcac
    10561 atcagtaaaa tcgctgtcca gcatggaaaa gatcaaacag aagaagaact gagactaatc
    10621 gttgaaaatg gtggtgaaat cattgatgta attgtggaac atccccttta cggtgagtta
    10681 gcaggaactt tgcacatcaa aacgccacaa gatatcgatt cctttatgag acgttatcaa
    10741 aaaagtaatg ctactttgtt atccgaatta acaggcggca tccatctgca tacgatccgc
    10801 tacccagaaa agcatatgct gaaaaacatc aaacaaaaac tagctgaagc aggaatcctc
    10861 tacgaaggat aacatcgtag ctgctacttt ttatcctaga aactattcta tcatctctga
    10921 gataaacacc catatggaag aaataagaaa aaataagaaa agatgcgtat ccaatacaca
    10981 gaattttttg gaattgcgat ttctagatgt ctatctaaat ttcgattagt ggagaaacat
    11041 aaaaaaggga agtgcagctt ttgagcactc ccctttttta taaagctttc ttgtagcata
    11101 gtatcgaagg aacctgccaa ttctctactc gatcaccaat acgccttctt ttagtgcaaa
    11161 tggattttga tcgttgatgt ggtcatagaa catcacaccg ttgatatggt cgatttcatg
    11221 ttgaacgacg atcgattcat aattcttcaa acgaatcttg tgtttttcac cattcatatc
    11281 ataataagac acagtgattt tggcatggcg aacaacatat cctggtactt ctcgatcaac
    11341 agataaacag ccttcacctt cccctaaaca agcatcttga acagaatggc tcaaaatttt
    11401 aggattatac ataacagtac ttagagaagg tccatctgcc tctgggtcag gacttggtac
    11461 gtgaacagca atgattcttt tagaaatatc taattgagga gcagctaagc caacaccgcc
    11521 tcgtaagtgt agttcttcag ctttgattgg atcttggctg ttttttaaga actcaagcat
    11581 ctcttttcct aaagagatat cttcttcgct taatggaaga gaaacttctt ttgcaacttc
    11641 gcgtaaggtc ggatttcctt cacgaatgat atcatccatt gtaatcatgg aatatgtacc
    11701 cctttcttaa aagactgtgt ttcaagtcta ttttcgcatt atagtttacc acaaaaatgg
    11761 aaaagtagta gtaagattgt ttgaaaacta ccttttctta atgattaact acttgatttt
    11821 caacccggtt tcaggtaaag tatagctgtg tgctctaggc acatgacctg ccacttggtt
    11881 aagcgaatca ccaacgtttt actcagtcgc aggggaataa tcgaaagagg tgaaagaaat
    11941 ggcaatttca aaagaacgca agaatgaaat catcaacgaa tacgctcgcc atgaagggga
    12001 tactggttct ccagaagtac aaatcgctgt attaactgaa gaaatcaacc acttgaatga
    12061 acatgcacgc gtgcacaaaa aagaccatca ttcataccgc ggcctaatga aaaaagttgg
    12121 tcaccgccgt aacttgttag cttacttacg taaaactgac gttcaacgtt accgcgaatt
    12181 gatcaaacgt ttaggcttgc gtcgttaatt ttagtcaaaa agagtgggat tcacatgaat
    12241 ctcacttttt ttactcgtat atcaattaag taaaattccg tgatcaagct agatggactc
    12301 ctactaacca aatgaaagct gctcaaaaga cggctttcgg cagttttcat ttgtttagta
    12361 gcgtcttgct ttcacgagaa ggaaaaggag aagttatgtc agaaaaacaa gtattcaaaa
    12421 cgacatgggg cggccgtccc ttacaaatcg aagtcggtca gctagcaaaa caagcaaacg
    12481 gagccgtttt agtccgttat ggagatacag tagtcttaag tgcagctgtg gcctctaaag
    12541 aagcaaaaga tacagacttt ttcccattaa cgatcaatta tgaagaaaaa atgtatgcag
    12601 cagggaaaat ccctggaggt ttcatcaaac gagaaggccg tccaagtacg gaagcaacat
    12661 tgactgcccg tttgatcgac cgtccaattc gcccgatgtt tgcagaaggc ttccgtaatg
    12721 aagtccaagt aacgaacatc gtgatgagtg tagaaacaga ctgctcgcca gctatggcag
    12781 caatgctagg ttcttcttta gcactatcaa tctcagatat cccatttgac ggcccaatcg
    12841 caggcgtaga agttggtcgt gtaaatggcg agtacgtctt gaatccaaca gtagaacaag
    12901 cagaacaaac agatatcgaa ttgactgttg ccggaacgaa acaagccatc aatatggttg
    12961 aaagcggcgc gaaagaagtt tcagaagaag acatgcttgg tgcattgctg tttggttttg
    13021 acgctatcaa agagttagtt gctttccaag aagaaatcgt tcaagcagta gggaaagaaa
    13081 aaatggaagt gactttactg caagttgatg aagtattgaa aaaagaaatc tttgatgctt
    13141 cttacgctac aatgaaagca gcagtaatga ctgaagaaaa acttgctcgc gaagataata
    13201 tcgagcaggt gaagatcgat atccgcgaag cttatgctga gaaatttgca ggtcatgaag
    13261 atgaagatca cttgttgaaa gaagttaaac aaatcacaga agatcttgaa aaagatgtcg
    13321 tacgtgaatt gatcaccatc gataagatcc gtcctgacgg gcgtaaattg gatgaaatcc
    13381 gtccattgtc atcagaggtc agcttattgc cacgtgtaca tggatcaggt ttgttcacac
    13441 gaggacaaac acaagcttta tcagcttgta cactcgcacc tctaggagag caccaaatca
    13501 ttgatggttt aggggtggaa gtcagcaaac gattcatcca ccactacaat ttccctcaat
    13561 tttctgttgg ttctacagga cgtgcaggct ctccagggcg tcgggaaatc ggacatggcg
    13621 cattaggtga acgtgcgtta gcacaagtaa tcccttcaga agaagaattt ccttatacga
    13681 tccgtttagt agcagaagta ttggaatcaa atggttcttc ttcccaagca agtatctgtg
    13741 ctggcacatt agcattgatg gatgcaggtg taccaatcaa agcgccagtc gccggtattg
    13801 cgatgggact tgtttctgat ggtgaaaact atacgatcct aactgatatc caagggctgg
    13861 aagatcatct tggcgatatg gactttaaag ttgccggtac aaaagatggt atcactgctt
    13921 tacagatgga tatcaagatt caaggaatca cagaacaaat cctaactgaa gcattaactc
    13981 aagcaaaaca agcacgtatg gaaatcttag aagaattgac ttcaacgatt gctgctccac
    14041 gtgaagaatt aagccaatat gcaccaaaaa tcgaaatgat ccagatcgaa cctgctaaaa
    14101 tcaaagatgt tatcggtaaa ggcggcgata cgatcaacgg aatcatcgat gaaactggtg
    14161 tgaaaatcga tatcgatcaa gatggaaaag taagtattgc ttcagctgat gcagaaatga
    14221 tcaaaaaagc tatcaagatc atcgaagatt tgaccaaaga agtcaaagtt ggtgaagttt
    14281 atctagggaa agttgtccgt atcgaaaaat tcggtgcatt cgtcaacttg atcaaaggaa
    14341 aagatggact tgtccatatt tcccaattag caaacgatcg tgtcaataaa gtcgaagatg
    14401 ttgttaaact aggtgatgag atccttgtga aagtaactga aatcgataaa caaggacgcg
    14461 taaatctgtc aagaaaagca atgttgaacg aagatggttc aacgaaaaat gaagaaaaat
    14521 aatttaattc ataggaataa tgtacttgtg atcgtgaagg tgacaggggg acaataatta
    14581 ttcgaaaaaa ggggactgtg acaaaagccg tgtcacagtc cttttttttc gaataaacgg
    14641 tgttcaaaag cttgcatctg cggtaataag tccagccaag ttaaaaacat taggagctgt
    14701 ttttatttgg ctgttcttat tacttgatgc agaacagctt ttttacaacc tctccaataa
    14761 acgatgttca atcagctgtc cctcgatatt agctcaaaaa acggcaaaat atgaggaact
    14821 atttttccgt ttgtttctct aatactcagg tcaaaccgct gatttcacaa cctctgattc
    14881 acggtgttcc agaagcagct tctacggaaa taagctgaac acttttgtta caacttcttt
    14941 ttttctggag tttcttggcg catatttttg gagtccttct gaataaagcg taaaataaaa
    15001 gtgagaaata tggataaagg agggaaagga gatgctgaat tttttatggt cgttatttat
    15061 cggcggtata ttaggtttga tagctggcgc aattttagga aaggaagtcc cgggaggcgt
    15121 atctggaaat atcattatcg gttttttagg tagttggctc ggcgaatttt tactaggacc
    15181 actgggaccg gaactcggcg gtttttattt gataccagca ttattgggtg caattttttg
    15241 ccttgctctt tattcatttg tggcaagacg tatcagagaa cgtccaaaga aagaataatt
    15301 ttattcaaat aagttgacac ttcagtgtgt tacttggata attagaagca atttaggaaa
    15361 gcgggtgtca tggataagaa tatcatctat cattcaagct tacctaaagg atatccatat
    15421 aacaagaaat gagggaaaaa cgtggcaaca acgcacaata ccaaaaagat atcaatgact
    15481 ggcctgcttg tagcgatggg agtcgtctac ggagacatcg ggacaagtcc gctttacgtt
    15541 atgaaatcga ttgttgaaga taatggcgga ttgtcaggaa tcaatcctga ttttgttatc
    15601 ggatcagtct cattgatttt ttggacattg actctactga caacaatcaa atatgtcgtg
    15661 attgcattga atgcagataa ccatggtgaa ggtggaattt tctcactcta cacacttgtc
    15721 cgaaaaggtg ggaaatattt gatcattcct gcaatgatcg gaggagctgc cttactagca
    15781 gatggggtat tgacccctgc tgtgacggta acgactgcga ttgaaggctt gcgaggaatt
    15841 ccgcagtttt ttgaccggtt cggtaataac cagaacgtga tcgtcattat cactttagtt
    15901 attatttttg ttttattcat ggtccaacgc tttggtacag atattgtcgg aaaagcattc
    15961 ggaccaatca tgttcgcttg gtttactttt ttaggcgtaa tgggattaat gaacttcagt
    16021 caagactgga cagttatccg tgcactgaat ccttattatg ctatccagct gctttttagt
    16081 gcagataaca aattaggctt gttcattctt ggaaatattt tcctggctac gacaggtgca
    16141 gaggcgcttt attccgatct tggacatgtc ggcaaacgaa atattcgtct gagctggcct
    16201 tacgtcaaaa tttgtttggt tatcaattat tttgggcagg ctgcgtggtt gttgaatgtt
    16261 tatcaaaacc ctgaagcaca gcaaatcaag aacctgaatc cattcttcca aatgatgcct
    16321 caaagttgga tcgtgatcgg cgttgctttt gcaacagtcg ctgcagtcat tgcttcgcaa
    16381 gcattgatta caggttcgtt tacgctagtt tcagaagcaa tcaagttgaa actgctgcct
    16441 cggttaaaga tcatgtatcc tgggaacagt atcgggcaaa tgtatatccc agcagtcaat
    16501 ctgattttat ggatcgcctg ttctctagtg gttgtcacat tccgaacgtc tcatcatatg
    16561 gaagctgctt acggtctatc gatcactgtg acgatgttga tgacaacagc actgctttac
    16621 ttttatctta tccaaaatgg gcattctcgt tggcttgcat atttagttac ctttttcttc
    16681 ggtgcgatag aagtcgtttt ctttatctca agtgttgtca aattcttccg aggcggtttt
    16741 gtagccgttc tgattggttt gatcatctta gtcgtgatgt ttatttggga acaagggaac
    16801 attattcgcg aatcgtcaac cgaggatgtg tctttgagag attatatccc gcagcttaga
    16861 gaattacgtg atgatacttc tttaccaatc tatcagacaa atgtggtctt cttggttcct
    16921 gatatggctg aagataaagt tggccgacag ttcatctatt cgattttaga taaacggcct
    16981 aaaagagcac gagtctattg gtttgttcat gtggaagtga cggatgagcc atatacgaaa
    17041 gaatatcaag tagatatgat ggacactgat tttattgtcc aagtgaatct gttcttaggt
    17101 ttcagggtcc aacaggaaat caacgtatat gttcgccaag tcatccatga tttgatgaaa
    17161 caaggccgtt tgccaaaaca gccacaaaga tactctctga caccagggcg agaagttggc
    17221 gatttccaat ttgtcctgat tgatgaagta gtttccaatg tgacaactct aggaaaatgg
    17281 caacgtcaaa tcatgcaggc aaaactggca atcaaaaaaa tcgctacctc gccagaaaca
    17341 tggtttgggc tagagtacag cgaagtaaaa catgaaactg ttccgcttat cattggaaat
    17401 actcggaaaa catggttgaa agagcggttg tgaaagtagc gctttgacct gaactaatgt
    17461 cgaatgtcag ctacttgaac accgtttatc agaggtcatg aaaaagctgt actgcaacaa
    17521 gcagtaagaa cgagcagata aaaacagctt ctcatgtttt tcatctgttt gggcttaatg
    17581 ccgtagatgc aagcttttga ataccgtttt tatcagaggt catgaaaaag ctgtttagca
    17641 acaagtaacg caatgatttt gaagtgataa ggagatgtgg ctgaaaaatg ccgcatctcc
    17701 ttttttattc ctaaatatga atacgcatta ttttcaatct ttttgtagaa aaagggtaca
    17761 atagaaaaag agaaaggcgg gagaatatgg ttatcaattt cattatttta tttttgatga
    17821 ttgcaattac ttcatttttt gtagcaagtg aatttgcttt agtgaaaatc agacgatcaa
    17881 gattggaaca actagaaaag gaaaatgtaa agaacgccaa gctagctctt catgttacac
    17941 accatttaga taactattta tcagctagtc aattaggaat tacgctaacc ggtttgatca
    18001 tcgggtgggt cggtgaagga tctgtcgcgg ctttactaga accagtgatc ggtaaactac
    18061 cttttagttc ggctattagc agtacaatct ctgtggcgct gggatttatt ttagttacgt
    18121 atatcgatgt ggttattggc gaactgcttc ctaagagcta tagtatcgtc aatacggaga
    18181 aggtcgtctt gttcgtagtt aaaccacttc attattttta taaagtaatg tttcctttta
    18241 tttgggtatt gaatcattca gctgcagggc tagggaagct attaggtgtg cgtctcgtat
    18301 ctgaaggaga agagacattg tctcaagaag agctgacatt agtcgcattg aattcttatg
    18361 aaaaaggaga gttgaccaaa gaagaatatt attatttgga aaacgtgttt gaatttgatg
    18421 ataccttggc aaaagatata caagtagacc gtacgtccat gcaagtattc gaagcttcag
    18481 aaaccgtcaa agaagcgata aaagcttcga ttgaacaagg acatactcgc tatccggtta
    18541 tcttagaatc aaaagacaat gttttaggtt atgtcacctt gccagatttg atcaagcaat
    18601 cttataaaga tgaccagctg acagtagaac agttgattga agagccgatc gtaacaactg
    18661 aaacgatccc tataaaaaaa ttattgacga tcatgcgaaa aaagggtaag catattgcta
    18721 tcttaaaaga tgaatacgga gggacaagcg gcttagtgac gattgaagat attttagaag
    18781 aaatcgtcgg ggaaatccga gatgagacag atttggacga agcattgata gcggaacagt
    18841 cagatggctc ttatatcatc tccggcaaac taacattaga tgatttccag cgttattttc
    18901 atgtagagat tccagagttt gaagaaacga attttactac acttgcgggc tttgcttcta
    18961 gccgatataa agaaataaaa gcaggaacaa tcattgagat tgcatcattc cgtttcacgg
    19021 tattagaata ccagcatgca catatcgatt atttcaaagt agagtccacg gaaagaaaaa
    19081 cagaataaaa gaaaaaaggc tgtgacattt tttgtcacag cctttttgtt atctaaaaaa
    19141 cctgttttcg gtaaacgaag aagatatttt caataaaagt tttcattttt agccgcaacg
    19201 atttctttta atcaaggcta tcttgcttct gcatgttcta atgtgaaatg ttcctcttca
    19261 cccatgtgtt tcaattgttt catcgaacgc atataagagt aaagcaacac ggcaacagaa
    19321 ccgatccaag ctaaaggaga agctgctgct gctcctgggt atccccacat tcctgtaaga
    19381 acgatagctg caaaagaacg cataagcaat tccatcatac cggcgatcgt agggatcttg
    19441 ctttgtccaa gcccttgaag tgtgtagcgt aaaatgaaca ggactgctaa gatccagtaa
    19501 agagacccat tgatattgaa gtaggtttgt gctaattcaa atacacgtgt ttctgatggg
    19561 ctaacaaaca attgaacaaa gaaatgaccg aagaaaatga cgatagcacc tgctaagaga
    19621 ctaaatccga cactcatcag taaagtttga cgtacccctt gcaagattcg gccatattct
    19681 ttcgcaccat agttttgtgc agtgaaagtt gccattgcaa taccaaatga catcatcggc
    19741 tgatttgcaa attggtcgat acgaccagca gcggcttgag cagctacgac atctgtccct
    19801 agactattta gtgctgcttg taacacgata gcaccaacag cgatgatcga agattgaaac
    19861 gccattggca aagcagcatt taggtgtaca gcaaactctt ctttttcaaa tttgaaatct
    19921 tttttgcgta actgtaacaa aggcattttt ttcttgatat acactagaca cagtacgctg
    19981 gaagaaactt gtgcgattac tgtagcaata gctgcacctt ccacacccat gccaaaatag
    20041 ataataaaga tcaaatcaag tacgacgttg atgatgaccg caatgatcag aaagaatagt
    20101 ggtgtgcggc tgtcaccaag tgcgcgaatg acattagaaa gcaaattgaa actgactgat
    20161 gcaaagatcc ctaataaaat aattgaaata aatgtttgtg cttgatcaag tatttcagga
    20221 ggagtttgca tcaattgaag catcggacga ataaacagga gacttaggac agttaaaatc
    20281 acagtgacga caagactaat gacaacacta gcagcaaaac ttttcttcag accccgataa
    20341 tcttttgcac cgtaacgttg tgcggtaata atggctaaac cagctgttaa accttgggca
    20401 aagccaatga tcaaaaatgt caggcttcca gttgcaccaa ccgctgccag cgcattttta
    20461 cctagcgtct gacccacgat gatcatatcg atcatattgt agaactgctg aaaaacattt
    20521 ccgatcagta aaggaacagt gaacatcaaa atcagtttag cggggcttcc ttttgttaaa
    20581 tcgcgcatct tttaaactcc ttatatcttt aaaatttagt tagtagtata tagcgtttat
    20641 cctgagaata ccaattagaa atcaatgata atttatgaaa ctccataaaa agaagccaag
    20701 aattgagtca aagaatcaac aacttggcct ggtctgtaaa ataagggaca tccacgttta
    20761 tctatcacct taatattgtc taatctcact tggtaaaagg atcgtttctc tcgtaccgtc
    20821 atcataggcg tatacttgag caggaaattg gttcgaatag gcaaacggca aatcaatatc
    20881 catttccaca ttgcttcctg cttgagaaaa atgcatcgtc acttttccac gattttcgat
    20941 caaatcaaag gttaatatat tttctaatgg aatgactccc ttaagatcta ggtcaataat
    21001 aaaccatata ctgtcaatca attcatctgg caaacttgat acgacaccaa ctgaagcgta
    21061 acggttgcgg ctgctatcaa aactttcaaa catctgttta acctccttcg aatcaacgaa
    21121 tgttttattt ttataattaa tcctaggata aactagaaat acttacttca acgagggttc
    21181 attctaataa agaatcagga aaaatgcaac aataattgaa tacttttttt caaagtataa
    21241 atttttataa aggtacgata aaagtatgcg aggaaaagct tggtaaatta ggatttttcg
    21301 tttgtaaagc catttacaaa agtggaagcc ccatatgaaa ataaacgatt agaaataaaa
    21361 aaggcgtgtt aaaagctgct gacccgccgc ttctaacacc acctgattgt ttcagtatta
    21421 ttcttctgtt gaaccgatca cttctggttc gccaccttca cctgtgcttt cttcttcttc
    21481 actttgagcg actacagcag cgatttgctc ttcactgtcg gtcacgaatt tgtaatcgtc
    21541 gttttttgga agatcggcta ctgtaataga atcacctaat tccaagtttg tcacgtcaac
    21601 ttcaattcgt tcaggcaatt tgtctggtgt agcagatacg actactgtat acaagttttg
    21661 agctaacact ccaccagatt tagcacccac tgattcgcca acaagaacga cttctgtttc
    21721 gacttctgtt tcttccgaca tgtttacaga tagaaattct gcatgttcga aatgattggt
    21781 aaaagtgttc gtttggatct tgtgaaccaa tgtattgact ttttgtccat cgatgtccat
    21841 cgtgatgact gtgttcgctc catgttcacg caacaatttg cttagtgtca aaccatcgaa
    21901 tgagataggg gtactttcta ctttataccc ataaacaaca gctggaactt tcccttcgtg
    21961 acgaagtttg tttcttaatg aacggggacg tacttctctt ttacttactt ctaatgatac
    22021 tgacataacc aaaattcctc cttaaaatgt gactgtctgc tttggtaacg gacagatttt
    22081 atttaagtga attctaaagg ctaatctcgt gcacatcatc gaaggcaggg cgcttaaaaa
    22141 agcgcaaaag gagagatgcg cgcatctccc cttagaaagt caggattcaa cttttctcca
    22201 ccaggtcatt acgctgtgta tgagaaatcc aattcacttt actatttaac cgtaacgtga
    22261 actgttaatt aagtcaagga ataactatga tgatgtattt gcaagcaaga atattcaagg
    22321 tataattaat tagtagtaat aagaaaggaa acagcatgcg tttagataaa ttaatagaag
    22381 aaaaattaga aacgacacgc aaggaaatga agcggctgtt tgctttgaag caagtgatga
    22441 tagatggcac agtcgaattt cgacaaaatc gaaatgtaga tagtttactg catcaaatcg
    22501 aagtagcagg tgaacactta gaaacaaatg agtcctatta tcttctcaat aaacctgaaa
    22561 aagctgttac agctgtatca gatccagaaa agacgacagt aatcgatctg attgcaagtg
    22621 ccgatcgttt ccaaccatta tatccagtgg gaagactaga tagagataca acaggactat
    22681 tgctgatcac ttcgaatgga caattgggat atgaaatgct acttccagaa aaaaaggttt
    22741 caaagactta tcaagtagtt gtgaacgaaa gggtgacacc ggcagacgta acagcatttg
    22801 aaaatggaat cgtattcgct gatgggtatc aatgtaagcc ggctgattta aaaatactca
    22861 aagcagacaa gcaccaaagt acggttttat tgacgatcca agaaggaaaa ttccatcaag
    22921 tcaaaaagat gttcttagcc caaggaaaga aagtgaccgc attgaaacgg ctaagcatgg
    22981 gaccattgga attagatgaa cagctaggag taggcgagta tcgatcgttg acattagcag
    23041 agatgaaaaa gttaagtatt tattttaaat aggaggtcaa aaagatgaaa tataaaactt
    23101 tattgtttga tgtagatgat acattgttag attttcaatt aactgaagcc aatgctttaa
    23161 gtgctttgtt tgcagaacaa ggaatcacat tgacaccaga aatcgaaacc agctataagg
    23221 ctttgaatca tcatttatgg caggagtttg aaaaaggcag actatcacga gaagaaataa
    23281 taggcagtcg ttttgggtta cttttccagc aatttgggaa aacagtagat agtgatcaaa
    23341 tggaaaaacg ctaccgccac tacttgaacc aaggtcatga tcttgttcca ggaagtctag
    23401 aaatcctgaa aaaggtccat ccgcatgctg atctttatat tgtgacgaac ggtgtgtcgc
    23461 acacgcaaca tcggcgatta acagatgcag gaatgcttga ttacttcaag gatatctttg
    23521 tctcagaagc tgtcggagcg caaaagccga tgaaagaatt ttttgatcat gtcttttccc
    23581 acattcctaa ttttgataaa aagcaaactg tgatcattgg tgattctttg acttctgata
    23641 tcaaaggagg aaatatggca gggatcgata cgatttggtt caatagaaat aaattaccag
    23701 aaatcgaaga aatccagcct acttatcgaa tcgattcatt agaggaactt tattcattat
    23761 tggaaattga aaaggattaa ttttcagaaa aatatagcat tttcgtgttt tttcgctata
    23821 atagattaga atgaaaaaac ggatacgaaa gaggttttag tcaatgacaa aaagcaagcc
    23881 aattatcatt ggtgtgacgg gtggatccgg aagcggaaag acaagtgtca gccgtgcgat
    23941 tttcaacaac ttcccggacc attccatcat gatgcttgaa caagattctt attacaagga
    24001 tcagagccat ttgagttttg aagaacgatt gaacacaaat tacgatcacc catttgcctt
    24061 tgataatgat ctgctgattc agcatgtagg cgacttactg aactataaag ccattgaaaa
    24121 acccgtgtat gactatgtag cgcatacaag aagtcaggca acgatcattc aggaaccaaa
    24181 agaagtgatc attctagaag gaatcctgat tttagaagac gaacgtctgc gtgacttgat
    24241 ggatatcaaa gtatacgtag atacagatga tgacattcgt atcattcgtc gtatcaaacg
    24301 ggatatggaa gaacggggac gcacattgga ctccgtgatc gaacaatatt tgacagttgt
    24361 taaaccgatg tatcatcaat ttatcgaacc aacgaagcgc tatgcggata tcattgttcc
    24421 agaaggcgga gaaaaccacg ttgctattga tttgattaca acaaaagtag ctagtttctt
    24481 aaaccataaa tagtgcgcag cgaaacagtt gaacggactg cgaccgtttg aaaagcaagg
    24541 attctgcttt ttgaacagtc accagccaac tgttttttta tttataacaa gaggttgtga
    24601 aaacagcatc ttgacctaag tattaaaaag aagaaacgga aaatagctgt ttatatttta
    24661 ccgttttttg agctaatacc gaaggtcagc tgattgagca ccgtgaagta gaggttgtaa
    24721 aaaagctgtc tttgcatcaa gcgataagag catccaaata aaacagctcc tcacgttttt
    24781 cgttgggttg ggcttattat cgtagatgca agcttttgaa caccgtttaa cgagaggttg
    24841 taacagaagt ggtcagatgc aataaataag aagaaactta cgaaaattat ttctcgaatt
    24901 tgtgtgagat ttcgacttat ttttttgaag aagctctttt gtcgcacccc c
//
LOCUS       DAZUMB010000033        24346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000033 DAZUMB010000000
VERSION     DAZUMB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.033"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..780)
                     /locus_tag="ACG80L_001118"
     CDS             complement(<1..780)
                     /locus_tag="ACG80L_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5023360.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKN"
     gene            complement(1004..2944)
                     /gene="thrS"
                     /locus_tag="ACG80L_001119"
     CDS             complement(1004..2944)
                     /gene="thrS"
                     /locus_tag="ACG80L_001119"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS5023361.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3281..3460)
                     /locus_tag="ACG80L_001120"
     CDS             complement(3281..3460)
                     /locus_tag="ACG80L_001120"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS5023362.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3507..5330)
                     /locus_tag="ACG80L_001121"
     CDS             complement(3507..5330)
                     /locus_tag="ACG80L_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5023363.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5608..7887
                     /gene="helD"
                     /locus_tag="ACG80L_001122"
     CDS             5608..7887
                     /gene="helD"
                     /locus_tag="ACG80L_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS5023364.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(7980..8627)
                     /locus_tag="ACG80L_001123"
     CDS             complement(7980..8627)
                     /locus_tag="ACG80L_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023365.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8806..9525)
                     /locus_tag="ACG80L_001124"
     CDS             complement(8806..9525)
                     /locus_tag="ACG80L_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5023366.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9656..10807)
                     /locus_tag="ACG80L_001125"
     CDS             complement(9656..10807)
                     /locus_tag="ACG80L_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS5023367.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11024..11764)
                     /locus_tag="ACG80L_001126"
     CDS             complement(11024..11764)
                     /locus_tag="ACG80L_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5023368.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11768..12091)
                     /gene="rsfS"
                     /locus_tag="ACG80L_001127"
     CDS             complement(11768..12091)
                     /gene="rsfS"
                     /locus_tag="ACG80L_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS5023369.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12148..12741)
                     /gene="yqeK"
                     /locus_tag="ACG80L_001128"
     CDS             complement(12148..12741)
                     /gene="yqeK"
                     /locus_tag="ACG80L_001128"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS5023370.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12725..13375)
                     /locus_tag="ACG80L_001129"
     CDS             complement(12725..13375)
                     /locus_tag="ACG80L_001129"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS5023371.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13385..13696)
                     /gene="yhbY"
                     /locus_tag="ACG80L_001130"
     CDS             complement(13385..13696)
                     /gene="yhbY"
                     /locus_tag="ACG80L_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS5023372.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13712..14821)
                     /gene="yqeH"
                     /locus_tag="ACG80L_001131"
     CDS             complement(13712..14821)
                     /gene="yqeH"
                     /locus_tag="ACG80L_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS5023373.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14818..15345)
                     /locus_tag="ACG80L_001132"
     CDS             complement(14818..15345)
                     /locus_tag="ACG80L_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS5023374.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15525..16310)
                     /locus_tag="ACG80L_001133"
     CDS             complement(15525..16310)
                     /locus_tag="ACG80L_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS5023375.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16303..17172)
                     /gene="accD"
                     /locus_tag="ACG80L_001134"
     CDS             complement(16303..17172)
                     /gene="accD"
                     /locus_tag="ACG80L_001134"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS5023376.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17173..18552)
                     /locus_tag="ACG80L_001135"
     CDS             complement(17173..18552)
                     /locus_tag="ACG80L_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS5023377.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18560..18979)
                     /gene="fabZ"
                     /locus_tag="ACG80L_001136"
     CDS             complement(18560..18979)
                     /gene="fabZ"
                     /locus_tag="ACG80L_001136"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5023378.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19004..19480)
                     /gene="accB"
                     /locus_tag="ACG80L_001137"
     CDS             complement(19004..19480)
                     /gene="accB"
                     /locus_tag="ACG80L_001137"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS5023379.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19483..20718)
                     /gene="fabF"
                     /locus_tag="ACG80L_001138"
     CDS             complement(19483..20718)
                     /gene="fabF"
                     /locus_tag="ACG80L_001138"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS5023380.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20737..21474)
                     /gene="fabG"
                     /locus_tag="ACG80L_001139"
     CDS             complement(20737..21474)
                     /gene="fabG"
                     /locus_tag="ACG80L_001139"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS5023381.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21486..22403)
                     /gene="fabD"
                     /locus_tag="ACG80L_001140"
     CDS             complement(21486..22403)
                     /gene="fabD"
                     /locus_tag="ACG80L_001140"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5023382.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22510..22734)
                     /locus_tag="ACG80L_001141"
     CDS             complement(22510..22734)
                     /locus_tag="ACG80L_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5023383.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22787..23749)
                     /locus_tag="ACG80L_001142"
     CDS             complement(22787..23749)
                     /locus_tag="ACG80L_001142"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS5023384.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23759..24199)
                     /locus_tag="ACG80L_001143"
     CDS             complement(23759..24199)
                     /locus_tag="ACG80L_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5023385.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6667 a         5423 c         4240 g         8016 t
ORIGIN      
        1 gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg ccaaaagtga
       61 attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta cgagtacatc
      121 ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt cgatattcat
      181 gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt ttgaaacaag
      241 ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt ggatatcttt
      301 ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt ctactactaa
      361 agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac actctacgac
      421 tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta cttgtgcatt
      481 gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat cactaaaacc
      541 ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg tcagactttg
      601 aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg aagcttctgc
      661 taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt agttggccaa
      721 ctttttttct gctgaagaat aatcttccat catactttca atacgactaa ttacgctcat
      781 aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga cccttattca
      841 cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat cttatcccat
      901 caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta tctattgttc
      961 ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt tctctgctgt
     1021 aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct acagctgttt
     1081 ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg tctccaacaa
     1141 ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc attttttcat
     1201 tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg atttcataag
     1261 cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata ggtgccaacc
     1321 atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt tccattgtag
     1381 aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct tctccaacat
     1441 atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat aatgtttctt
     1501 ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag gctgcctcgc
     1561 cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc attgcttggg
     1621 ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga tcgcgatagc
     1681 tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc atcaattcaa
     1741 gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga ccatcattca
     1801 acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca tagcgatgca
     1861 tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc gtgtttttgt
     1921 agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg ccatctccca
     1981 tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat gttttgtaca
     2041 actcaacatc tcccattatt ggtgtatata catgttggta acctagacta acttctttgt
     2101 ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc cagaatggca
     2161 aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct agtttacgat
     2221 ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct ttcttgtcaa
     2281 aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca cgccaataag
     2341 cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga acatgtggtc
     2401 cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct tcttctggca
     2461 attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat gcttcctgtt
     2521 ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc atttctgctt
     2581 cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta tcgtagtaaa
     2641 accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg cgacgcatgg
     2701 cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct tcatggtctg
     2761 gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca acgagttttc
     2821 cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt tcagcaattg
     2881 ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa gtaattttta
     2941 tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct gaaaaacaaa
     3001 aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt tccacctaat
     3061 gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa
     3121 gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc tagcttttcc
     3181 tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta tgtgtttatt
     3241 taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc cattccccat
     3301 ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt tcttttggtg
     3361 cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct aattgttccg
     3421 gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct cctctttctg
     3481 atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac tgaatcagga
     3541 aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt atccacgact
     3601 tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat taacatcaaa
     3661 aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat cgtatctctt
     3721 gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac cgcatctgca
     3781 ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc tgcagcagca
     3841 agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg atctttaggg
     3901 atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac agaatctaat
     3961 tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat catcatcaaa
     4021 tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg gcgaatctga
     4081 tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat tgctgtattg
     4141 gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac ttcatgatta
     4201 ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac aggatacagt
     4261 tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga ttcctgttct
     4321 gcactagctg catacagaag tagctcttct ttttttattc cattgactgg ctgaatcgcc
     4381 tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat cgtttttgta
     4441 cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt acttgaacgg
     4501 ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg tgatgccact
     4561 accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat accaccgatc
     4621 aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac aaatcgggca
     4681 ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct gtctgcggca
     4741 cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga cataatctga
     4801 tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc atcgaccgaa
     4861 gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt gactagtaac
     4921 tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc tttgaatcga
     4981 tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc tcgtttattc
     5041 gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa actagcccag
     5101 tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc tacgccatat
     5161 cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag aatccctcca
     5221 gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc acttaataaa
     5281 gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat tgttttttct
     5341 cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct ttcatcttaa
     5401 gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac aactatcact
     5461 atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag gattactttt
     5521 tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt ttaattcgtt
     5581 atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca aacgcacgtg
     5641 gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa acaacaagaa
     5701 ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat tcgagctggt
     5761 tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca ggaattgctt
     5821 ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa
     5881 gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga aacattatat
     5941 ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga ctggcgcgca
     6001 ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga aactgaaaca
     6061 gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga aggacgtatt
     6121 ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga gatattggat
     6181 gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc acaaaatgca
     6241 atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg aagtggaaag
     6301 acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa gtggctcgag
     6361 gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat ctcgactgta
     6421 ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta tttggtgcag
     6481 ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt tttgctaggg
     6541 gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca gattgatcgc
     6601 tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat aaatggccgt
     6661 acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga acaattacct
     6721 ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg cggtctgcaa
     6781 aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct ccaagaacta
     6841 tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa
     6901 ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat catgaattat
     6961 caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc actttctctt
     7021 ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac tgttcgtaca
     7081 cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt actgatgcgc
     7141 cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga tgaaatgcag
     7201 gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc aaatttgact
     7261 ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa
     7321 gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta tcgctccaca
     7381 aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt agaaacaaca
     7441 gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat caaattgaat
     7501 tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg tacagcaatc
     7561 atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga cgagttgaaa
     7621 caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat tattttactt
     7681 ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga tgtagatgca
     7741 gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac acgtgcgatg
     7801 catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa tatggatatt
     7861 tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt aatgttagca
     7921 gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt taaggtcatt
     7981 tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag cgaaataatc
     8041 attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct atgacggcca
     8101 atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg gtcaattgat
     8161 tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg aaatgctagg
     8221 tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct ttcgataaga
     8281 attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc ctccatccga
     8341 tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga
     8401 taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa ataacggttc
     8461 agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa tcttgcgtat
     8521 ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac tttgcggtaa
     8581 ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt cttccccgtc
     8641 catcttccac atacttcata tcatttcaca tgatacttat gctctcatca taccacaggt
     8701 tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag tgagattgtc
     8761 ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt cttctaatgc
     8821 cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa tcattttttc
     8881 aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca tttggatctc
     8941 agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac tatggaaatc
     9001 cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc gaacatcgat
     9061 gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa aagcaaagat
     9121 tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc cgccattttt
     9181 cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac
     9241 aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt atgtttcatc
     9301 tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta cattataagt
     9361 tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag gatcacctga
     9421 ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat tattagcatc
     9481 tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt cacttcgctt
     9541 tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg tgaatcacga
     9601 ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca attatctata
     9661 ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat tgatagattt
     9721 gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt cgggaataaa
     9781 tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat ccaagtagtc
     9841 gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc agcaagacat
     9901 aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc tttgacacaa
     9961 aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct attccttcgg
    10021 tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga taacgcaaat
    10081 acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct tctgtctgag
    10141 gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg gctgttgcac
    10201 tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta cgtcggatcg
    10261 gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt aatcccaaga
    10321 tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga aaaacctcag
    10381 tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc tcttctatcc
    10441 tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa gtctcttctt
    10501 ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact gcacctttag
    10561 caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact aaatccacac
    10621 cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt tcgcctcttt
    10681 gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt tttcgtgcca
    10741 tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata atcccgcagc
    10801 ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac aagtaaaaaa
    10861 aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac acaatacttc
    10921 tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata taatttatgt
    10981 tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta
    11041 caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt
    11101 cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat ggataggtac
    11161 gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt tcttcaacga
    11221 aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta tcccacaaaa
    11281 aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca tctacttgat
    11341 aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga tagacttcat
    11401 caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca gaaaaacagg
    11461 taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc acaaattgga
    11521 tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct tctgaaaggt
    11581 ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct ccagtaccgc
    11641 atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc
    11701 attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat gtttcataag
    11761 ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac catagttttt
    11821 ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca tctccaagat
    11881 cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca tatttgtttt
    11941 cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg
    12001 aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa gcgataatgt
    12061 cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct ttactatcta
    12121 tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat tatatgtttc
    12181 gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt
    12241 ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc
    12301 aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt caaggagact
    12361 catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg catcggtgat
    12421 tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg cattattata
    12481 attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact cctcatctgg
    12541 tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag gagaagcacc
    12601 gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct tgaagcgttg
    12661 ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag cagtatattc
    12721 attactatag tccatattca tacaaccctt cattttgaat ataatcaatt actttatctg
    12781 gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc gtggaactga
    12841 tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt gtgtatcctg
    12901 gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta taccattttg
    12961 gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc ggattgtttt
    13021 gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct aattcaatcg
    13081 tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg agtcgatgtt
    13141 ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc atcaaaaata
    13201 cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt gcaaggtgaa
    13261 cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag aattgaggca
    13321 tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc atcatggtca
    13381 ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt gtggaaggtt
    13441 tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt tctttttcta
    13501 atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat actttgatca
    13561 gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg atcccacctt
    13621 ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt
    13681 ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat
    13741 ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg atccagccta
    13801 gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga acaagctccg
    13861 gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca tgtttttgat
    13921 aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt gacacataag
    13981 caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa
    14041 gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac ggtgcaatga
    14101 tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata attcccggtg
    14161 tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag gtcgttcctg
    14221 gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg atttgattga
    14281 taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt ccatcgcggt
    14341 attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca ctagttaata
    14401 atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat tgtgttaatt
    14461 tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa agtactggat
    14521 tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca aaaatatcca
    14581 ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt
    14641 catccgtcaa tgagacatct tgggtctcat tataatggcg taatctgaaa caacgctgac
    14701 aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt
    14761 caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat tcttctgtca
    14821 ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg ccataattct
    14881 tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa tgggttttac
    14941 aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa tctgatcgcc
    15001 taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt tgatccctct
    15061 tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa tcgctcgcgc
    15121 gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat ttttcatttc
    15181 taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa tcaacgtatt
    15241 gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag gtgtaatctg
    15301 atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt ctcctcatct
    15361 gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg catatcagca
    15421 aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta caaaatgacc
    15481 aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata ttggcggaac
    15541 ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc cgtcaatctt
    15601 tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg ctcgccgttg
    15661 aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg cgcagtaatt
    15721 ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat
    15781 ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc atccgctaaa
    15841 cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat gatcggtacc
    15901 tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc gcgctcttct
    15961 gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg tctattgaac
    16021 ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg gggagaacca
    16081 aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat cactgttacc
    16141 ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc aaataaacga
    16201 tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa gcttgtccat
    16261 cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac gatctatcca
    16321 tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt ccatgcgggg
    16381 aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga aatcttctgg
    16441 aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg
    16501 gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc ctccggtcgt
    16561 cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt tgacagcagc
    16621 agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg cgccacctga
    16681 agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata atctggtgat
    16741 cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca tcactccaat
    16801 aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat gaagtcctgt
    16861 tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg gatctttggt
    16921 tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag ctagacgctg
    16981 ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt caccgatgtc
    17041 tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat caggaacaga
    17101 tggcgagtta gcaggtttgc tctctctatt aggattgatc cgaatatatt tctttttctt
    17161 aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg gtaaaaattc
    17221 ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac taatcaaatc
    17281 catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta aggctcgctg
    17341 catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg caatcattga
    17401 atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc gcagtcccat
    17461 cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa aatggaaagc
    17521 aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta cttcttcttg
    17581 acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta aatcgattcc
    17641 tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt ccatgaaata
    17701 aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat aattgactgc
    17761 tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg agatcactac
    17821 agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc
    17881 taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat ggcgagcagg
    17941 ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg cttcttgctg
    18001 agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa tgcctttccc
    18061 gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg ctatttccaa
    18121 tgcttcttct actgtagata atacaccagt acttcctgga atgactggga catttgcttc
    18181 ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg cacttttagg
    18241 accaataaat gtgatattgc actcttcaca catggaggca aatcggctat tttcagataa
    18301 aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc
    18361 ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg cttcatctgc
    18421 taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg tttggatccc
    18481 tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag ctatcaatac
    18541 tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg gctacttttt
    18601 taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact ttcaatatct
    18661 caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt gctttgtcca
    18721 atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat gcaactgctc
    18781 ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct tctggaaaat
    18841 gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc cgttctcctt
    18901 caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga atgatttctt
    18961 ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt tgctgaatcc
    19021 taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt tctgtgatca
    19081 ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata atgcagataa
    19141 cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt
    19201 gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat actgccgatt
    19261 ctttttcttt cattatttct ggagcagttg ttgcttgttc aggtatgaca ttgactggct
    19321 ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt gttttatttt
    19381 tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc gaggattgat
    19441 caaattgact gactaattct ctgatttcat tgatatccaa aattattcct cccagcgttt
    19501 catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg cataagtaaa
    19561 acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat cttgtttttc
    19621 tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg ccaaggcttc
    19681 gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt
    19741 atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct ggtcattggc
    19801 tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta caccagcttc
    19861 ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag gagctgtcat
    19921 atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt tggcaccgcg
    19981 agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac ccatcacaaa
    20041 accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg attctgagag
    20101 tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt ctgttcctcc
    20161 agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc caattgaatt
    20221 cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac ctacacgcaa
    20281 agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg gagaaactcg
    20341 ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa gaccaccgat
    20401 cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat ctaaaccgct
    20461 catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat ccattcgttt
    20521 gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac ccgctaaggt
    20581 aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg tcccctcttt
    20641 taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc catagccagt
    20701 aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga ccaatccgcc
    20761 atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa ctgctgtttg
    20821 tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg cttgttcctt
    20881 gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg caatggcatt
    20941 acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc ctactacgcc
    21001 agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag atacactaga
    21061 gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt gctgcgtcat
    21121 attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg catccattcg
    21181 catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga tcgatccaag
    21241 cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga tgtcaccgga
    21301 aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt ttggaatctc
    21361 tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg ccatcgcttt
    21421 gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat ccataacgtt
    21481 cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac acgcagaact
    21541 ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg gccgacttcg
    21601 atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa acgaacaggt
    21661 tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat ttccgttgtt
    21721 gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag gtccttcgcc
    21781 agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac atttagaggg
    21841 atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac tgctttttct
    21901 tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac gatgccgtac
    21961 ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc
    22021 attttcccgc ttccagctgg ggcagcttct gtcatataag cgcctctttt tgctactaac
    22081 gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata ttctcctaaa
    22141 ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg ataatacgcg
    22201 atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg ttcattttct
    22261 gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc aaatgtctgc
    22321 tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc accttggcca
    22381 ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata tgtttgattc
    22441 ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc cccaacctaa
    22501 ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa ccgttttgat
    22561 tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga tgatttggaa
    22621 taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc
    22681 agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata ccataataat
    22741 atattcctcc gatattgttt gctttttatt tttataattt aacagtctac aactccatga
    22801 gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc tgctgttttc
    22861 ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg cttgcagcag
    22921 atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc tttacttttc
    22981 gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa tcaactggtt
    23041 tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa tcaaaaatat
    23101 ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag aacggatttt
    23161 tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct gctaattttt
    23221 ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct ccatcgccaa
    23281 acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt tcaccaccaa
    23341 tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt aacgcataaa
    23401 caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct cccaatttcc
    23461 cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt gctactaaaa
    23521 taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc gccactaaaa
    23581 tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata cccgtccttg
    23641 acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta gtaataatct
    23701 tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt aaatatcctc
    23761 acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt
    23821 cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa tgctgatgga
    23881 cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg cggtcatctt
    23941 cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca gtggtcaagg
    24001 tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc ttgtacatcc
    24061 cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga gattttttca
    24121 gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa tcatttactg
    24181 tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc aaatcttttg
    24241 atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca tttttttact
    24301 atatgagctt tttttaagat tttactcatt taaaacaact gataaa
//
LOCUS       DAZUMB010000034        24012 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000034 DAZUMB010000000
VERSION     DAZUMB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.034"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..410
                     /locus_tag="ACG80L_001144"
     CDS             135..410
                     /locus_tag="ACG80L_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS5023386.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            416..1183
                     /locus_tag="ACG80L_001145"
     CDS             416..1183
                     /locus_tag="ACG80L_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS5023387.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1351..3180
                     /gene="typA"
                     /locus_tag="ACG80L_001146"
     CDS             1351..3180
                     /gene="typA"
                     /locus_tag="ACG80L_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS5023388.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3441..5213
                     /gene="recQ"
                     /locus_tag="ACG80L_001147"
     CDS             3441..5213
                     /gene="recQ"
                     /locus_tag="ACG80L_001147"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS5023389.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5327..5629
                     /locus_tag="ACG80L_001148"
     CDS             5327..5629
                     /locus_tag="ACG80L_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS5023390.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5632..6795
                     /locus_tag="ACG80L_001149"
     CDS             5632..6795
                     /locus_tag="ACG80L_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5023391.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6821..10249
                     /locus_tag="ACG80L_001150"
     CDS             6821..10249
                     /locus_tag="ACG80L_001150"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS5023392.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10270..11394
                     /locus_tag="ACG80L_001151"
     CDS             10270..11394
                     /locus_tag="ACG80L_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS5023393.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11375..11821
                     /locus_tag="ACG80L_001152"
     CDS             11375..11821
                     /locus_tag="ACG80L_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5023394.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11805..12143
                     /locus_tag="ACG80L_001153"
     CDS             11805..12143
                     /locus_tag="ACG80L_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS5023395.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12262..12822
                     /gene="rsmD"
                     /locus_tag="ACG80L_001154"
     CDS             12262..12822
                     /gene="rsmD"
                     /locus_tag="ACG80L_001154"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS5023396.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12819..13310
                     /gene="coaD"
                     /locus_tag="ACG80L_001155"
     CDS             12819..13310
                     /gene="coaD"
                     /locus_tag="ACG80L_001155"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS5023397.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13300..14343
                     /locus_tag="ACG80L_001156"
     CDS             13300..14343
                     /locus_tag="ACG80L_001156"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS5023398.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14502..15230
                     /locus_tag="ACG80L_001157"
     CDS             14502..15230
                     /locus_tag="ACG80L_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS5023399.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15346..15591
                     /locus_tag="ACG80L_001158"
     CDS             15346..15591
                     /locus_tag="ACG80L_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5023400.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15588..15806
                     /locus_tag="ACG80L_001159"
     CDS             15588..15806
                     /locus_tag="ACG80L_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS5023401.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15942..16481
                     /locus_tag="ACG80L_001160"
     CDS             15942..16481
                     /locus_tag="ACG80L_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS5023402.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16474..18747
                     /locus_tag="ACG80L_001161"
     CDS             16474..18747
                     /locus_tag="ACG80L_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS5023403.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18785..19831
                     /gene="holA"
                     /locus_tag="ACG80L_001162"
     CDS             18785..19831
                     /gene="holA"
                     /locus_tag="ACG80L_001162"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS5023404.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19920..20174)
                     /gene="rpsT"
                     /locus_tag="ACG80L_001163"
     CDS             complement(19920..20174)
                     /gene="rpsT"
                     /locus_tag="ACG80L_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS5023405.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20574..20843)
                     /locus_tag="ACG80L_001164"
     CDS             complement(20574..20843)
                     /locus_tag="ACG80L_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023406.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21105..21233)
                     /locus_tag="ACG80L_001165"
     CDS             complement(21105..21233)
                     /locus_tag="ACG80L_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023407.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(21313..21768)
                     /locus_tag="ACG80L_001166"
     CDS             complement(21313..21768)
                     /locus_tag="ACG80L_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS5023408.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21906..23834)
                     /locus_tag="ACG80L_001167"
     CDS             complement(21906..23834)
                     /locus_tag="ACG80L_001167"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5023409.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8002 a         4119 c         4994 g         6897 t
ORIGIN      
        1 gaaaaatcag ttgcgttttg ttttttagaa ttatcaataa tattattatg taaacttaaa
       61 aatatgacaa cttcttggtt tattaccact tcctatgcta cactgaagag gaatatgaaa
      121 taggagagtt gaagatgcaa gaatatcagt atccacttga tttagattgg acaacagacg
      181 aaatggtcat tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa aaagggataa
      241 acaatgaagc ttttttgaag acttatcagc agtttaaaac tgttgtcaaa tccattggtg
      301 aggaaaaacg attgggacgg gaatttgaaa aagcttccgg ttattcttta tatcgtacag
      361 tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga gataaatgga
      421 acagatgatt gatcatatca agtcctggct tgaagaagct ggtgagaaaa tcaaatcttc
      481 tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa caaatgtcga
      541 tcgaaacatc cagaattttc taatcgaaaa aatccattcc tttgatccag aggcgaaaat
      601 attggcagaa gaagaagggt gtaatcaatt atcggatctc gatggaagag tctttatcat
      661 cgatcctatt gatggcacct tgaattttgt gattgaaggg gaaaattttt gtattatgct
      721 cgctgtttat gaatccggcg ttggaaagct tggtttcatt tatgacgtga tgagaaatga
      781 acttttttgg ggtggaaaag gaataggcgt gtataaaaat gatgagccat tgcctagacc
      841 agcaaataaa tccttatcgg aaggcttact tggtgtgaat agttatcttt tcggtcataa
      901 taaatatgga atccgagaaa ttgcggaaag cagcatgggg acacgtatgt acggatgcgc
      961 tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt gcttatattt caaaccttag
     1021 cccttgggat tatgcagcgg gaaatgtctt attggaagag tttggtatgg taacaagcgg
     1081 tctttttggt actcctttga catttgaaaa aagggaatac tatttagcag ggacttcttg
     1141 tgcatatgca gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag gtagaaatca
     1201 ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg aaaacatttt
     1261 actatatttc atgaaaaaag tctgctataa ttagcagtcg agtaaaattt gccctgtcat
     1321 aaagaaccaa taaaaaagga gttagttaca ttgaattata gaaacgatat ccgtaacgtc
     1381 gcaattattg cccacgttga ccacggaaaa acaacattag ttgatgaact attaaaacaa
     1441 tcagatacat tagacgcaca tacgcaattg caagaacgtg cgatggactc aaatgcgcta
     1501 gaaaaagaac gtggtatcac gattttagcc aaaaacacag ctgtagacta taaaggtatt
     1561 cgcgtaaaca tcatggatac tccaggacac gcggacttcg gtggcgaagt agaacggatc
     1621 atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac aatgcctcaa
     1681 acacgttttg tattgaaaaa agcactggaa caacatatca caccaatcgt tgtcgtaaac
     1741 aagattgaca aaccttctgc tcgtcctgag catgtcgtag atgaagttct tgaactattc
     1801 atcgaattag gagcagatga tgatcagtta gactttcctg tgatttatgc atctgcacta
     1861 aatggtacgt caagcttgtc agatgatcca gcagatcaag aaccaaccat ggcacctatt
     1921 tttgatacaa ttattgaaaa aatccctgct cctgtcgata acagcgacga accattacaa
     1981 ttccaagttt cattacttga ctacaacgat tatgtaggtc gtatcggaat cggacgtgta
     2041 tttagaggaa caatcaaagt aggagaccaa gtagcattaa ttaaattaga cggaacagtt
     2101 aagaaattcc gtgtaacgaa attatttggt ttctttggac taaaacgtct agagattcaa
     2161 gaagcaaaag ctggagactt gattgctgtc tcaggtatgg aagatatctt tgtcggtgaa
     2221 acagttactc ctgtggatca tcaagatgcg ttacctatcc ttcacattga cgaaccaaca
     2281 ttgcaaatga ctttcttagt taataattca ccatttgccg gtcgagaagg taaattcgta
     2341 accgctcgta agattgaaga acgtttgatg gcagaacttc aaacagatgt atccttacga
     2401 gttgaaccaa caaactctcc agatgcatgg acagtttctg gtcgtggaga attgcatttg
     2461 tctatcttga ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc tcgtccagaa
     2521 gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt gcaaatcgat
     2581 acaccagaag aatatatggg tagtgttatc gagtccttga gcttacgtaa aggtgaaatg
     2641 caagatatgg tccatacagg aaacggacaa atccgtttga cattcttaac acctgcccgc
     2701 ggtttgattg gttattcaac tgaattcctt tcaatgacac gtggttacgg aatcatgaac
     2761 catacttttg accaatatct gccaatgctt cctggtcaaa ttggcggacg tcaccaaggg
     2821 gcgcttgttt cgatcgatac aggaaaagca acgacttatt ctatcatgag tatcgaagaa
     2881 cgcggtacag tcttcgtaga accaggtaca gaagtatatg aaggaatgat tatcggggaa
     2941 aacagccgtg ataatgattt gactgtcaac atcacgaaag caaaacaaat gacaaacgta
     3001 cggtctgcta ctaaagacca aacatctgtc atcaaaaaac caaaacaatt gacgttggaa
     3061 gaatctttgg aattcttgaa tgatgatgaa tattgcgaag taacaccaga aagcattcgc
     3121 ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa gaaaaaataa
     3181 tctgtaaaag gatgaagaga gaaaccagct tgttataaaa aatagctggt ttcttttttt
     3241 gtccaactct tttctgtgga gaagtcatct ttgtcaatgg acataaattc agaaaatacg
     3301 ccgaaagacg tttaaaagac ttttaaaaaa cttatttcta gagagttttt gtttcataat
     3361 aagataatgt ttcacgaaag gagatcaact tgtccttcga agaaaatcga agtaaaatgg
     3421 caagagaaag gagcgggaag atgaacgact tattaaaaca atactttggt tatgatgagt
     3481 ttagacctgg tcaaaaagaa attatccaga aagtcattga tcaggaaaat gtattaggaa
     3541 tcatgcctac cggaagtggg aaatcaatct gttaccagct tccagcttta ctattagatg
     3601 ggcttactgt ggttgtgtcc cctttaatct ctttgatgaa ggatcaggtg gacgcggcga
     3661 atcaattagg tatacccgcc acttttatca atagctcgtt agatggttac gaaacagcta
     3721 gacgcttcca agaaatcgat agacagcagt accgtttact ttatatcgct cctgaacgtt
     3781 ttatcatgcc ggattttatc caagcaatga agcgatggaa cgtccgtatg atcgctattg
     3841 acgaggcaca ttgtatctct cagtgggggc atgatttccg cccaagttat ttacagatgg
     3901 cgaaccagct agatcaactg ccaaatcgtc ctgtaattgt tgctttgaca gctacagcga
     3961 ctgtgcaagt agctgctgac ataaaaagac ttttaaaaat tccagaaaac aaccatattc
     4021 agactggttt tgaacgagaa aatctacgat ttcaagtaat taaagatcaa aaaaaagaac
     4081 aatatctaat cgaatattta aagatcaaca aaaatcaatc tggtatcatt tatgcagcaa
     4141 cgagaaaaga agtagaccgt ctctatcatt tgcttaaaaa attcgatttt tccgtgggta
     4201 gatatcatgg tggtctaaat gagaatgagc gaacagaaat gcaagaagcc tttttatacg
     4261 atcgcttgca gctgattgta gcgacaaatg catttggtat gggtataaat aaaagcaatg
     4321 tccgttttgt tatccattat caaattcctg gctctctaga agcatactat caggaagctg
     4381 gtcgggcggg aagagatggt ctttccagtg aagccatcct gcttttctct cctcaagata
     4441 tccaagtcca aaagtttttt atccagcagt cccaaagaga agaagggcaa aagcaaaaag
     4501 aatatgaaaa attaaaagcg atgacagaat acgtgtatat tgagtcctgt cttcagcaat
     4561 acatcctaaa ctattttggc gagacaagca gtccgtgcaa ccgttgtggt aactgtttag
     4621 atgacagaga aatagtagaa gtaacgacag aagcacagat ggttctttct tgcttaaaac
     4681 gtatgggtga gaactatggt aaacaaatgc tgatgaaagt cttagcagga tcgaaagaac
     4741 aaaagctgca agctttaggt tttggacatt tatctaccta tggactgatg aaaaaccaat
     4801 cgcaaaaaga aacgatgcag ttaattgaat atttgatatc gaatggctat cttctgacca
     4861 tcaatggtga gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta ttgaaaggac
     4921 aagaatctgt ttatcgaaag gaacccaaga aagtccagca gctatcggat gaagaaaccg
     4981 atacactatt tgaagtgctg cgggaactgc gaacggatct agcatcagaa gcaggcgttc
     5041 ccccgtatgt cgtattttca gattccacat tgaaagaaat gagccgaata cgtccatcaa
     5101 gcagattgga gatgcttcag ataaaaggag ttggccaaag caagcttgat aaatatggcg
     5161 aagccttttt atctcggata aaaaatgcgg actctaacgt tctggataag tagaaagaac
     5221 gaatacgtaa aaatgaataa aaacaactgg ttaaatctgt tttttttgct ccttatgaaa
     5281 atcaaatatg ttataattaa ggaattagaa tgggaggtaa gcacagatgg attctttgtc
     5341 gaaaaaactt gcgatccaag tattaaatga tgaagcagaa cggatcaaac gattaatccg
     5401 aaatcaaaac aacagtctct gtatctcaca atgcaaagcc ttcgaagaag tggtagatac
     5461 acagatgtac ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag taatcggaaa
     5521 gaacgagggc cagctgatgt taagtgagct agaacgagaa ttgaatcagt tatatagtga
     5581 ggtttaccaa gaaatttatg ataaaaaaga agttggcaag gaggaataga cttgcctaag
     5641 aaagtgaaaa aaaggcatct attagactat agcatcttga ttccctattt aattttatgt
     5701 gtattgggat tgatcatggt atatagttct acgtcgtatc tattattaga aaacggacaa
     5761 aatccgtccg catcagtcat taaccagagt attttttggg tgcttagctt gattgctatc
     5821 gcactacttt ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat catggcagcc
     5881 atcgcagttt tgacgatttt gctgctgatt gttgtgtttt ttggtaagga gatcaatggg
     5941 gcgaaaggtt ggctgcaaat tgcaggattc tcgatccaac ctgccgaata cctaaaaatc
     6001 atttctatct ggtacctgtc tctgacttta tctaaaagac aaaacagtgt acaaaaagat
     6061 tttcttggga ctgtgaaacg tccacttgca atggttatcg gacttactgc attagtcgct
     6121 attcttcccg attttggtaa tgcggcagtt atctttttga ttattttggt tctgctttta
     6181 gccagcggag tcaattatgt gtatactttg attgtaggag taggaggctt ttgtctaagt
     6241 acttttacga tctggctgat caatatcaca aacggaaaga tcttacccgg acgtttacag
     6301 tatatctata atcgatttgc tatttaccaa aatcctttct cagacgaatt aaataaaggg
     6361 catcaactag tcaacggcta ctatgccatg ttcaacggag gactatttgg caggggatta
     6421 ggtaacagta tccagaaaaa aggattttta caagaagcac aaaccgattt tatttatgca
     6481 atcgtagtgg aagaactggg agtgattatg gggattttga tattagcgtt gctattcttt
     6541 atgattgtgc gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa ttcgcttttg
     6601 tgcattggga tcggcgcaat gtttttgatc caagtatttg tcaatcttgg aggaattact
     6661 ggcgttattc ctttgacagg gatcacgttc ccatttttaa gtcaaggagg ctccagtttg
     6721 ctgatgcttt cgatttgtgt cggctttgtc ctcaacatca gtgcggatga aaaacgcaag
     6781 agcttaggtt tataataaaa aaagatgtag gagtggttcg atgaaaaaag ttttagttgc
     6841 taacaggggt gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag gaattaaaac
     6901 tgttggaatc tatgcagaag aagatgaata ttccgtacac cgtttcaaag cggatgaagc
     6961 atatttagta ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg aagggatcat
     7021 ttctatcgcg aaagaatgcg gagctgacgc tattcatccg ggttacggtc ttttgtcgga
     7081 aaacttgaat tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg gacccaaact
     7141 gcatcattta gatatttttg gagataaaat caaagcaaaa gctgcagcta tcgaagcggg
     7201 gattgcttcg attccaggca cagatggacc aatcgcttct atagacgatg ccttggagtt
     7261 tgcgaaacaa tacggttatc cgattatgat caaggcagct ctaggcggcg gcggacgcgg
     7321 catgcgtgta gctcacgatg aaaagagtgc aagagagggc tacgaacgtg cgaaaagcga
     7381 agcaaaagca gcctttggta gtgatgaagt gtatgttgaa aaatacatcg ctaatcctaa
     7441 gcatatcgaa gtacagattc tcggggatac gcatggaaac gtcatccatc tttttgaaag
     7501 agattgttct gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt gtgtctctat
     7561 gaatgaacag caacgtcaaa aaatctgcca ggcagctgtc caattgatga aatatgtcgg
     7621 gtatgtcaac gcaggtacag tagaatttct tgttgaagga gatgattttt actttatcga
     7681 agtcaaccct cgcgtccaag tggaacatac gatcacagaa atgattacag atattgatat
     7741 cgtaacaacg caactgttga ttgctcaagg tttggatctg cataaagaaa tcggattgcc
     7801 tcagcaagaa ggaattaaat taaatggttc agccattcaa tgccgaatca ctacagaaga
     7861 cccgttgaat aactttttac cagacacagg gaaaatcgat acgtatcgtt cacctggtgg
     7921 ttttggcgta cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga caccgtattt
     7981 tgattcattg ctagtaaaag tatgtacgca tggtgctacc tttgaaacag ccattcagaa
     8041 aatggaacgt tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca ttccatttat
     8101 gctcaatgtc atcacacatc ctgaattcca atcaggaaat gcaaaaacaa ccttcattga
     8161 tagtacagca acattatttg aatttcctcg actacgagac cgcggaaaca aaacgatgaa
     8221 atatatcggt gaaattactg tgaatggttt tccaggtatc gaaagtggcg aaaaaccatt
     8281 ttatgaagaa ccacgtatgc ctaaagatct aatcacaaga actgattacg taacggctaa
     8341 gaatgtatta gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag gacaggaaaa
     8401 tttgttgttg acggatacga catttagaga cgcccatcaa agtctgctag caactcgtgt
     8461 tcggacaaag gatttcaagc agattgcacg tttgactgga gaaggtcttc ctgaactctt
     8521 ttcaagtgag atgtgggggg gagcaacatt tgatgtggct taccgctttt tgaatgaaga
     8581 tccttggcaa cggttgagaa aaattcgttc tttaatgcct aatacgttgc tgcaaatgct
     8641 tttcagaggt tcaaatgccg ttgggtattc taattatcct gacaatgtat tggtggaatt
     8701 tgtcaaagaa gcagcagcgc aaggaatcga tgtatttcga atctttgata gtttgaactg
     8761 gactccacaa atggaaaaaa gtatccaagc tgtacgggat actggtaaaa tcgcagaggc
     8821 agctatttgc tatactggcg atatcaacga tccaagtcgg gcaaaataca atgttcagta
     8881 ttataaagac atggcaaagg aactagaaca actgggtgcg catatcattg caatcaaaga
     8941 tatggctggg ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat tgaaagaaac
     9001 gacagatctg ccaatccatc tgcatactca tgataccagc gggaatggaa tcatcactta
     9061 ttctgctgct tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg caatgagtgg
     9121 gaatactagt cagcctagca tgagcagctt gtattatgca ctggtcaatg gtccacggct
     9181 gccagaaatc acgattgaaa atgcacagaa attaaaccat tattgggaag atgtgcgcat
     9241 gtattacaaa ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat atatgcatga
     9301 aatgccagga ggacagtatt cgaatctaca gcaacaggca aaagctgtcg gattaggtca
     9361 tcgctgggat gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg gcgatatcgt
     9421 taaagtaact ccatcatcta aagtagttgg agatatggca ctatttatgg tccaaaatga
     9481 tcttacagaa gaagatattt atgaaaaagg cgaaacacta agtttccctg aatctgttgt
     9541 gactttcttc caaggagaac tagggcaacc cgttggcgga tttcctaaaa aattgcaaaa
     9601 gattattctt aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg ctgaatcagt
     9661 cgacttcaac gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac caaaacaaga
     9721 cgaagtatta agctatttga tgtatccgca agtctttttg gactatcaaa aagcatataa
     9781 tcaatttggc gatgttacat tactggacac accaacattt ttccaaggta tacgtttggg
     9841 tgaaacaatc aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt tagatgaaat
     9901 cggagaaccc gatatcgaag ggaatcgcgt actgttcttc aatttgaatg gacaaagacg
     9961 tgaaatcact atcaaagata attcgatcat cagtacggtc caaacccgat tgaaagctga
    10021 accgacaaac cgagaacaaa ttggagcaac gatgtctggt tctgttttgg atgtattagt
    10081 gaaaaaaggc gataacgtta aaaaaggtga tacgttgatg attactgaag cgatgaaaat
    10141 ggaaacggcc atcgaagcac gttttgacgg agaagtcgca catgtatatg tgtcttcagg
    10201 agacactatt tcgtctggtg accttttgat tgaagtgact gagaaataat aagcgaaagg
    10261 tggggaaaca tgaaaagact gggtttgttt tggctgattc tgctattagt tattgtggca
    10321 ttctacatgg aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa ctctgatcaa
    10381 caaatatcaa accaaatgac aacgtggaaa taccaggagc ttaaagcaac tggtttttct
    10441 acttatatcg gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc ctatgatcga
    10501 ttaactagcg gatttggttt tgaaacaaga tattatgaaa ataaagctag tcatctcaca
    10561 tttgaagcga acatccaaga cagcagagtc acaactgtta aagtactgca aacagggcct
    10621 gctgatatcg cgccgtttta tttagggatg acgatgcaag atcttacgaa aatcactact
    10681 atctatacta atttcacttt tgagtaccat gatacagaag ttggaatcga actaatggaa
    10741 gaggatatga attatcgtcc gctaattgct tttgataacg acacttttgc tattttattt
    10801 tttgatcaaa caacaaacgg cctattttca gttgtgtatt tagataaaga tagtcttttg
    10861 aaactattgc catatcaagt gtttggtgaa ggccttccac attatcaagc agataaatcg
    10921 gctgattggg aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt attaaatatg
    10981 cttagaagag aagacgaact tcccatatac aatcaaacac ttgcctttca aaaccacagt
    11041 cagctgctac tacatgattt tttgacaagg cctgaggaaa tattgtcaga ggaacgtatg
    11101 gctgattggc aaaaatcgct aggcagtgct caaacgacag tgaaattctc actgacgaat
    11161 gatgaattgg agaagttaat cagtaaacaa aaattagaaa atactagtgg gctatttatt
    11221 catccggcga ttgacgctac tttttcgttt ttatattggt acagcgatcc ttacaaccat
    11281 gaccgtttca tggacccggg aacggattct gtaggcattg ctttttctaa agaaaatatg
    11341 ttagtcttat tgcaagaaga aaacggagaa agtagtgata gcagtgatcg ttaacgaaga
    11401 gctgtttcaa ctggaggatc aatgcctcca agtcgctgaa atgataaaaa agagtaaaac
    11461 gatggcttct tatcttgaac ataaacagtc aatggaagaa tctgcaaaag tttgccagtt
    11521 gaaagaagcg ttcttgtcta agaaagaagc atttgaaaat gtagcggcat atggtgagta
    11581 tgcacctgat tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac agcttgatat
    11641 ggatgagcat gttgcagctt tccgatatag tgaaacacag ctgcaaagtc tgttggatca
    11701 aattgtccaa gtacttgccg aagaagtttc taatgaaatc aaagtagatg caggtagccc
    11761 gttttttgaa acgaaaaaac actttggatg cggaggtaat tgtcatgcaa ttagacaata
    11821 gtgaatttga aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc ttaaaacaat
    11881 taaagaatct gaaaaagttc ggcttgttgc attatgtctc aagaagaatg aaatatgtcg
    11941 tgctttactt aaatgaagag acattggaag aaacagaaga acgaatccgt aaattacatt
    12001 ttgttcgaaa cgtagaacgt tcttaccgac cggatatcga aatgaatttt gccgaaaaaa
    12061 taggaaacaa aacagtatta aaagacgaag gtttcgaaat tgaagagcta aatacagaaa
    12121 tccgattagc tgaacatctt taaaaatctc ttatgagcag tttttaaata aaaaataact
    12181 tggataacga tcgatttttg atcattgtcc aagtattttt ttggtatact agtaccagta
    12241 ccatttctaa ggagtgagta gatgcggata atatcaggaa attatggcgg gcgccgttta
    12301 aaaagtttag caggggcgaa caccagacca actacagata aagtaaaaga atccatattc
    12361 aatatgattg gaccatattt tgatggagag acagtattgg atctgtttgc aggaagcggt
    12421 ggtttagcta tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt agacaaaaat
    12481 taccaagcgc taaaaatcat caaagaaaac atcgaaatta cgaaagaacc agagaaattt
    12541 accacaataa aacttgatgc ggacaaagca atcgaacggc tagcagcgga gaaacaacaa
    12601 ttcgactact tgtttcttga tccgccatat gcaaaacaaa aaataatcga tcaaatccat
    12661 aaaatggaag aactgggact tttttctaaa accgcagtta tcgtatgcga aacggataaa
    12721 acagtcgaat tgccagaaac tgtggctgga ttccatcaaa taagaaaaca gatttatggc
    12781 ataacggccg taactatcta tagaaaagag ggaaatcaat gaaacgtgca ttattcccag
    12841 gaagttttga tccttttaca aaaggtcact tagatacggt ggaacgtgcg gctaaattat
    12901 tcgatgaggt agtaattggt gtttttatca atacttcaaa gaaaagtttg tttcccccag
    12961 aagaaagaat gacattgatc acaaaagctg tttctcatct tcctaatgta aaagtcatgc
    13021 atcaagaaaa tcaattgacg gtagaaacag caaaagagat tggtgcagat gcgttgatca
    13081 gaggcatacg aagtatcaaa gactttgaat atgaacgaga gattgctcag atgaatcatc
    13141 atctatatga tgaattggaa actgtcttct tattagcaaa accagaatat agtcacgtga
    13201 gttccagtat tttaaaagaa gtactgcact ttggcggaga tgtgagcagc tacttaccgc
    13261 cagtgatcaa cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa acaatggttg
    13321 aaaacagtac tgattatcat atttggaatg ttgatcattg gatttgtagt agttcccttg
    13381 ccgtattatg tagaatcacc gggagccacg atcgatctga aagaactaat tacggtcaat
    13441 gataaggaag ataaaggttc tgggtctttc tctcttacct ctgttggtat caggcaagct
    13501 acaggtttct tagctttaaa agctaaaatg accccttttg aagaattgat cagtgaagaa
    13561 gaattgactg gtggggcgac aaatgaagaa tataatcaaa tgcaacagta ttatatggat
    13621 tcttcccaaa atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc ttataaaatg
    13681 gaattcaaag gcgtatatgt catgagtgta gagaaaacat cgaatttctt tggcaagctt
    13741 tcagttggag atacagtaac aaaagtagac gggaaaagct ttcaaagtac agaagcattt
    13801 atggattatg taaaaagcca aaaagttgga caaacaatcg aaatcagtta tttgcataat
    13861 ggtgaagaaa aaacagcttc tggtgatctg atcgagttac caacagataa aaaggcagga
    13921 ataggtatca cgcttactga ccataccgag atcgaatcag atacagatgt tcgtattaat
    13981 tctggctcta tcggtgggcc atcagcggga ttgatgttca ctttagaaat ctatgaacaa
    14041 gtcactggta aaaacttgcg acatggtaag caaattgcag gaactggaac gatcaacagt
    14101 gaaggagaag ttggccgaat cggaggaatc gataaaaaag tggcaagtgc ggataaagct
    14161 ggcgtagaga ttttctttgc accagatgat acgatttcag acgatgttaa gaaagaatat
    14221 cctgaaataa aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt gaattcctca
    14281 atgaaaatcg taccagtaaa aacagtacaa gaagcgcttg actatctaga gaaaatgaaa
    14341 tgaaactaaa taattaaaga ggggttgtga cacgagttct gtcacaaccc ctctttttcg
    14401 tcaaaaatct tcacgataaa aataaaaaca aaagaaaaaa ttgacgtaat tagatgttga
    14461 agggacgatt ttgccagagc tcactgtttc ttcaatttta aatgaaagga gtaatgaatg
    14521 atttttactg gtcattcttt gaaaaaagaa caatgataga aaaaatcagc aagaaaatac
    14581 tagcttcacc caaattgatc atttggatgt ctatgatagt tcttatcttt tgtatcgcaa
    14641 gtggtttttt attatcaaat ttgctatttt atagagaaac agaagagact gctgcaaaag
    14701 aaatactttc ttcgtttagt tccagtggag aagatccatc agctgaagga aacgatgtag
    14761 aagaggatgc tcgtccaaag gtcatgtaca ccgatatcaa aggaagtgtg aaggagcctg
    14821 gtatctattc cttttcttca gaagaacggg tctatgatgt actaaagcgt gcaggtggat
    14881 tattagaaga agcggatagc gatcgaatca acttttctgc gaagatagaa gatcagcaag
    14941 tcctatatat ccctgctgta ggtgaagagc tgccagaaca tcttgatcag ttggcatcct
    15001 cggaaggaaa acagcctaca gcagattccg agcctcccaa aataaatatc aatacagcca
    15061 ctccatcaga acttcagcag attcctggga tcggatcagt caaagcgcaa gaaatcattc
    15121 gatttagaga agaaaacgga tccttccaga aagtggaaga cctgcaggag atttctggta
    15181 tcggtgaaaa gacagttgaa aaactaaaaa acttcgttac aattaaatag aatgattcaa
    15241 aatcgactgg cacgagcaat aaatggacgc taatggatac taagaataat ccaattctaa
    15301 gagcttggca ggacgaggag gtagtatttt tggataggaa atagcttgat tgttcgcaaa
    15361 gaaacattaa aaaaaccaat gctgaatgtc tatctccaaa ataaaatatc tgggattcat
    15421 attatgaata cggctgtcag cggcaataac agtcaagctc tacgagaacg ctttgccaaa
    15481 gatgtactta gctatacagc agataaagta tttattctca ttgggacgaa tgatctagca
    15541 gagcataaac aactttctaa agaaacgtac caaaaaatct gcagtggatg atagttcagc
    15601 tgaaaaaaga ctatagttct gatcatgctt tctttatcac gccaccagca gtcgatgaga
    15661 caaaacaaaa atatcggaat aacaagttga tcaaagaata tatctctgtt ctcacagaag
    15721 tagcaaaacg ccatgattgt gaagtatcga tttttatacg gaatttcttg aacaaatgga
    15781 ttcgaagaat ttttataatg ggctaatgga tgacggactt catttcggca gtgaagggta
    15841 ggatcttttg tcagacagga tgattcgtta tatttaaaga atattttgtt taagcaatga
    15901 gtacttttta atcaacttga aaaaacggag gaatataaaa catgttagaa aaatcttcaa
    15961 aagcatgcga tacgaataaa gaaaaagtta ctgataagtc acataatgaa cgcattcctt
    16021 gggaccagta ctttatggct caggcagtac tgttatcttt acgtagtacg tgtacacgtt
    16081 tagaagtagg agcgacactc gttcgagaaa aaaggatcat tgctggcggc tataatggag
    16141 cagtcagtgg agatgtccac tgcatagatg aagggtgtta cgtcgtggat ggacactgct
    16201 tgcgaacgat ccatgcagag atgaatgcac tcctacagtg tgcaaaactt ggtatcccga
    16261 cggaaggttc agaaatctat gtcacacatt ttccatgttt ggcctgtaca aaggctttgt
    16321 tgcaagctgg tgtgaaaggg atcaattatc tccatgacta tcgaaatgat ccttatgccc
    16381 aagcgttgat cgaacaatta ggtgtatctg tacaccaagt tgctttagac tcacagcatt
    16441 tttcaaaact tcaaaatgaa ttaacaggaa aacatgtcta gcgttttaga aaggcatgtt
    16501 cgaaatcggt tgatttttcc ggcaattacg tcgatgatta tctgtttgtt gtattacact
    16561 gacaattggg gttatcgttt gcttcttgcc ggaatttttt tatggttttc gctaaaagaa
    16621 ggacgtttta tcgcgtactt gtgtattttg tgtagcataa cggtatccat ttcttatagg
    16681 ctacaagagt attcattttt agaggaaaaa acaacatctg cgcaaattac tgtatttaca
    16741 gatacgattc gtgcaaatgg taattttttg acaatggaag gaaggttatc agagaataaa
    16801 caaaagctcc agctttctta tcaagtaaac acagaagcag aattgaaaac atggcttaac
    16861 ttccgaggaa agggaataga attgactgct gaaggcgagt tcatctctcc agcaaaaaag
    16921 cggaatcttt ataattttga ccaagaagct tacttcagga accatagaat ttctggacgg
    16981 ttccaaattc aaaagattga acagatcacc atgcaaaatc actggcgaaa ctggcttcag
    17041 agaaaaagag gatcttttat ctcctttgtc caatctgaat ttccgcaaaa aacaagtgta
    17101 tatattaatt ctttattatg gggctataaa gatgcaggat tcagagaacc agaagaaatt
    17161 tttagggaga gtggtttgct gcatctgttc agtctatcgg gtctacacat ccaattttat
    17221 ttagggtggc tgtattattt atttcgacgt atcggatggc cactacacgt cagtattatt
    17281 ccgttgagtc tactaactat ctgttatgtt tctattgcag gaagttctat ttctgttttg
    17341 cgtgctagtt tgctgttttt attgcgattg ctagtcaaat tgctgaataa gcattattcc
    17401 accatggact tatttgcagt tgtcttttgg cttcttttac tatttgatcc tcgatttctt
    17461 tttcatgcgg gtggacagct tagttttttc atgagttttt tgtttatcct gatttctttt
    17521 gatggagatt ggttgaaaaa aacggcctct cacgtcctgt ttactatgat cacgatgccg
    17581 ttgattgtat ggcattttta tgagtggcct attttaggta gtctttttac attactgatt
    17641 gcaccggtat tcaagtttct ttttcttcct ggtgtgttat ttctcacact cttcaatcgc
    17701 tggctgccgg aaactttact tttgctgata gaagagggat taatcttatt tgagcgaata
    17761 atcgattttt cttcagggtc gtctctcatt ataggacgtt tgccccttgt gctatctggg
    17821 atgtatattt tcggattact gatttgtatg gataggttga aagaacaacc tgtgaaacac
    17881 ttgttttatg ttggtctttt ttcttttatt tttttgactt tgccattttt acgcttttct
    17941 tcaacgatcg cttttatcga tgtcggtcaa ggagacagta tctttttgca aagtcctttt
    18001 cgaaaagaaa cgattttgat cgatactgga ggtcagttac actttgaaag agaaaagtgg
    18061 gcgcaaggta tgatacgtcc tcctgctgaa agaaatatcg ttccatatct aaaaggtcag
    18121 gggataagtg taatcgataa actcatcttg acacacgacg atacggatca tgtgggagaa
    18181 ttacccacgt tatctcagca ttttactatc aaaaaaattt atatcggttg gggcgctgga
    18241 cgaaatgagc cattacaaag gcatttatct gaagctcaaa aaaatggtac tgagatcatt
    18301 gaaatcaaac atggggacag gatcagcggt tattatgatc tttatgtatt gactcctttt
    18361 gaaaagggag agggaagaaa tgaagattcg attggtctat ggatggaata taacaatagg
    18421 cgctttttat ttttaggaga tttgaatcaa gcaatggaaa aacaattgct tttgatttat
    18481 cctaatctga aagccgatgt agtcaaatta ggtcatcacg gtagccgtac gtcaagtaac
    18541 acagattttc tttcccatat tgaagcaaaa catgggatta tatcctgcgg cgtaaataat
    18601 cgttatggtc acccgcacag cgaagtattg gagacactag aagaaaatca gataaaaaca
    18661 tggcgaacgg atcaacaagg aatgatcacc tatcgctggc atccagtttt tcatcctcat
    18721 gggctagtgg aaacaatgat agactagtaa ttctcatctg ctcatggtag gataattttt
    18781 gaaagtggtg agtaatttga gtatacaagc agagttacaa aaaatacgta aagaaaaact
    18841 tgcatcttgt tacttgattt taggaacaga aaagtttctc caagatcaag ttcggacaga
    18901 aattgtcaaa aaaatccaat tagagggcga agatgactta aattttttaa gttttgacat
    18961 ggaaaatgca agtttagatg aggtagtagc tgaagcagag acgttgccgt ttttcggaga
    19021 tcaacggtta gtattcgtag agaatcctta ttttttaaca ggtgaaaaag taaacaatgg
    19081 aatcgaacaa aatactgatt tattgacggc ttatttgaaa gaaccattag aatcgactgt
    19141 attagtattc tttgcgccat atgaaaagtt ggatgaacgg aaaaaagtaa cgaaacaatt
    19201 gaaaaaaaca gcaattatca tcaatgtcca acagttgaat gaaaaagaag tacgtcaata
    19261 tctgatgaat accttagaga attcctcaat caaaatggaa cgaagtgcaa ttgatttatt
    19321 cttgaggttg accgacttag atttatccaa aatgatgaga gagttacaga aattggtcct
    19381 ttatggacaa aatcaacagc aaatcacggt aagagaagta gaagaattag ttcctaaaac
    19441 gttagaacat aatatttttg acatgaccca atatatttta aaagggaaga cggaacaagc
    19501 tttgcgttta tacgaagatt tagttatgca aggggaagaa acaatcaaaa ttaatgcgat
    19561 tttgctttct caattacgtt tatttttaca gacaaaattt cttgtaaaga ttggttatca
    19621 gcaggcaaat attgctgaaa cattgaaaat ccatccatat cgtgtaaaac ttgccatgca
    19681 ggaagtaaaa aaattcgatg aacatttgct ggtacagttg tttgatcaat tagttgagat
    19741 ggattatcaa atcaaaacgg gacaaatcga aaaagaatta agttttcaac tattcgtttt
    19801 gcgcacaggg cagttgttaa gaaatcgata agaagaaaga cgagggcaaa gcatgcggga
    19861 aggtcgtttg tttttgtcct ttttttgaca aataaaaaaa tcggccgaag ccgattcttt
    19921 tatttagcga tttttttgct taaacgagat ttgtcgcggt tagctttgtt tttgtggatt
    19981 agaccttttg attcagccat atcaatagct ttaacagctt ctttatataa cgcatctacg
    20041 ttgtcagcac ctgaagctac agcatcttca aattttttga ttgcagtacg catagcgctt
    20101 gtttgagatg aatttttcac attagcgttt tcgcttgtac gaacacgttt gattgcagat
    20161 tcaatgtttg gcattagttt cacctccgat aacttaagtt cttacgcgtg ttaccgcata
    20221 attcaacatt cattattata cctaaaggca gcgtgctttg caatatgaac atgacaagtt
    20281 tttttcctta acagtctttt attaggattt tttttcagta aaaacggata gtacaagctg
    20341 aaaaaaatca aaaagacgct attgtgggag gcaatagcgt caaaccaaac tagattttta
    20401 tttatctatt taaacaaagt ttgaatggga atgtttggta ctaagttatt atataacaaa
    20461 gagaaccgtt tgcaaagttt tttttgaaaa gggatacgat tccctgttgt tctttgtttt
    20521 tctattaaga aactgcagtt attttataac cagtatcctt tgatcagtaa aggctattct
    20581 ggtgtaccga ttattttaat gtattcatcg atatttttta tcaacatttt ttttatgacc
    20641 aactggttcc gacggttcca tttgccactt acttccagca catatttatt taggggcaaa
    20701 cgtaagatat attttgccaa atagcgatct ttaagcaggc cattgatcac acggtcctca
    20761 agctgcaagt tgatcccagt cagcatcatt ggctgttctt gtaaaacgta cgtatcaatt
    20821 aaaagacctt caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa cctgaaaaca
    20881 ggcactaatt cttccatttt gatattcgtt gaagtggatg cgatcattct ttcttttgtc
    20941 cactgtaatc tagtataaca aaaaagagca gaagagccaa aaagaaaaaa gagcatcgaa
    21001 ttctatagga agtctcattg gcacggccta aattattaca aaacattgat taaaaaaagc
    21061 ccctgcatcg caggagcatc ccctatatcg ttttaatagg tcaatcaaaa agcagtatca
    21121 aacacatttt gtatcaaatc aggaaataat agataagcta agcccagaat caaaagtacc
    21181 gctagaagaa accagccgat tgttcctatg aaatacttta atttttcgaa cattgtatca
    21241 cctcgttgtc agtatatcac ggatcagtaa attgggggtg ttaagaatct cctaaaatag
    21301 acaaaatttg ttctattttc ctaaagcaaa tacttgtaag atacagtaag tgataccaga
    21361 agcaatgacc ggtccggcgg cgattcctct taagaagaca acacctaaaa tcgtgccgaa
    21421 aacaagtgca acagtgattt gtggatcaga agcaatcaat cctacaccct tggaagataa
    21481 gacagccact agcccaccgc aaaaaacagc tacccagcca ataggggatt taaaggcttc
    21541 taatagatca cgtagaccga tttctcctgt tgcgataggg attagaatag ccgctgtgat
    21601 gatcgtcacc ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg aacgggaggc
    21661 tggtatgagc ttcataatca aaacaaaagc tgccgctatc atcagacttt gatttttagc
    21721 taaaattgct acaagtaaaa taattcctaa aaaaagccaa ctttccatga aatatattcc
    21781 tcactttctt cgaaacaatc ataacatgtt tttagaaagt tacaaaagta ggaattcatc
    21841 cttcaagacc tattcataag ctgttttttt cataaaataa acaaaatcat aaaaaaacac
    21901 tttgcttatt tgacagaaga cagccgcgct ttgtatagtg gacaagactt acattttttt
    21961 gaagaatcag aaagatcagg taaaggtctt agtaaacgca agccattcag aataaccaat
    22021 attgtgcttc cttcgtgtcc tacaactcca agaggtagat ttaaaaattg caggaagtta
    22081 gaagcaatca acaagagaat aacaagaatc gaaaaaataa tattttgttt gatgatccgc
    22141 cgcattttta aagatagccg atgactgtag acaaatttat ttaagtcgct tttcattaag
    22201 acgatgtctg cgatatccat agcggcatct gtcccttgcc ccatagcgac acctaaagaa
    22261 gcggctgcta aagcaggcgc atcattgact ccatcaccaa ccatcgcatt tacttggtaa
    22321 agttcattct cttttttgac tagttgtgtt ttttcttccg gagtacagct tgcatagtaa
    22381 tgttcaatcc ctacttgttc agcaatggcc tttgctgtgc cagaatggtc gccggttatc
    22441 atacttgtat ggatgttttg ggatttaaag taggaaatta cttgttgggt atttgctttt
    22501 ggaatatcta gcagacctaa aacagcaatt aactgctgat ctttagaaag ataaatcacg
    22561 gttttgcctt gattttccaa acgttcgatc atttccgcaa tctctttgct gattatcatc
    22621 gtctcaggat catatgcgtg cttgccaact ttccattgcg cacctaaata ggtagtttgt
    22681 aatccaaaac cagtaatttc ttctattggt aagtattgga tttcttcagg tattgtgcaa
    22741 tcgaaatatt gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc catagcaaca
    22801 agaagttgtt gtgccaaatc cttttcatca aagaaaaagc tgtcagttac aactggtttt
    22861 cctttagtga gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga aagcttttct
    22921 aaatggatac cgcctttgat taaaatccca ttttttgcag aatttgaaag agcagctaga
    22981 gaagctggtg tagcagatgc gacaagcgca caaggagaag ccacaactaa taataccatt
    23041 cctcgataaa agctttcatc ccaactccaa cctaggaaaa agtgggggag aaggatcatt
    23101 agaggaacag caagcaaaat caattttaca taattatttt cgattcgttc gataaatgaa
    23161 gctgtttgag ttggcgtatt ctgcgcttct tctactaatt ggataatctt agcaaagaca
    23221 gtttcggaac tcgtttttgt tactcgaatc gtgatgggat ttccttgatt cagcgttcct
    23281 gcatacatct catcgcctaa atatttttct acaggcacag attcaccgct taatgcagac
    23341 tcatcgatcg ttgcctgtgt gctttctaaa aagccatcta tcggaacagc tgctccttta
    23401 gcgacaaata ccaagttatc cacctttagt tgagaaacat ctacttcttg catttgtcca
    23461 ttttctttta ataattgggc tttttgtggt tgaagattca tcaaagcagt aatttctttt
    23521 tgacttttat tagttgtgta ttcctctaat gcaccgctta aacaaaagat gaatgtcaac
    23581 attgctcctt caaagtaatt accgatcaaa caggcaccaa tagcagctaa agccatcaat
    23641 aaatccacat tcagtgtccg atcattccac aattctttca agccttccga tgtttgttta
    23701 tatccaccga tgataatggc actgatataa aaaagtgggg tgatggtaag agagaatcgg
    23761 tcgaagataa aaccgaagat cataaataaa aggcagccga ttgttgctgc aaaagctttt
    23821 ttctgattag tcatcgttat tagacctcct tgtcaaaaca aggatagcat aataaaaatg
    23881 taattgataa taataacagc taaatgagaa cgataatggt tatcatttgg tgattaagct
    23941 tgatgttgtt tttattattt ataatgatta ttattaaaga aaataaaaaa ccagaacatt
    24001 tctcaaataa ga
//
LOCUS       DAZUMB010000035        23830 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000035 DAZUMB010000000
VERSION     DAZUMB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.035"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG80L_001168"
     CDS             complement(54..788)
                     /locus_tag="ACG80L_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5023410.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG80L_001169"
     CDS             1115..1525
                     /locus_tag="ACG80L_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS5023411.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG80L_001170"
     CDS             1518..2219
                     /locus_tag="ACG80L_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS5023412.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG80L_001171"
     CDS             2219..3805
                     /locus_tag="ACG80L_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5023413.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG80L_001172"
     CDS             3824..4045
                     /locus_tag="ACG80L_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS5023414.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG80L_001173"
     CDS             4042..4803
                     /locus_tag="ACG80L_001173"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS5023415.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG80L_001174"
     CDS             5183..5692
                     /locus_tag="ACG80L_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5023416.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG80L_001175"
     CDS             complement(5767..6306)
                     /locus_tag="ACG80L_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS5023417.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG80L_001176"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG80L_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS5023418.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG80L_001177"
     CDS             7319..8170
                     /locus_tag="ACG80L_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS5023419.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG80L_001178"
     CDS             8198..8833
                     /locus_tag="ACG80L_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS5023420.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG80L_001179"
     CDS             8853..9626
                     /locus_tag="ACG80L_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5023421.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG80L_001180"
     CDS             10034..10831
                     /locus_tag="ACG80L_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS5023422.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG80L_001181"
     CDS             10809..11222
                     /locus_tag="ACG80L_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5023423.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG80L_001182"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG80L_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5023424.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG80L_001183"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG80L_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS5023425.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG80L_001184"
     CDS             15999..16559
                     /locus_tag="ACG80L_001184"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS5023426.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG80L_001185"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG80L_001185"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS5023427.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG80L_001186"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG80L_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS5023428.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG80L_001187"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG80L_001187"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS5023429.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG80L_001188"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG80L_001188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS5023430.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG80L_001189"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG80L_001189"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS5023431.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG80L_001190"
     CDS             21906..22484
                     /locus_tag="ACG80L_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5023432.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG80L_001191"
     CDS             22536..23018
                     /locus_tag="ACG80L_001191"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS5023433.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACG80L_001192"
     CDS             23135..23509
                     /locus_tag="ACG80L_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023434.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6649 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DAZUMB010000036        23064 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000036 DAZUMB010000000
VERSION     DAZUMB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.036"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(110..565)
                     /locus_tag="ACG80L_001193"
     CDS             complement(110..565)
                     /locus_tag="ACG80L_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023435.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="ACG80L_001194"
     CDS             complement(719..1927)
                     /locus_tag="ACG80L_001194"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS5023436.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="ACG80L_001195"
     CDS             complement(2039..2371)
                     /locus_tag="ACG80L_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5023437.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="ACG80L_001196"
     CDS             2519..3394
                     /locus_tag="ACG80L_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5023438.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="ACG80L_001197"
     CDS             complement(3476..4057)
                     /locus_tag="ACG80L_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS5023439.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="ACG80L_001198"
     CDS             complement(4063..5322)
                     /locus_tag="ACG80L_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5023440.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="ACG80L_001199"
     CDS             complement(5324..6370)
                     /locus_tag="ACG80L_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023441.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="ACG80L_001200"
     CDS             complement(6605..6880)
                     /locus_tag="ACG80L_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS5023442.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACG80L_001201"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACG80L_001201"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS5023443.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACG80L_001202"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACG80L_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS5023444.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACG80L_001203"
     CDS             complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACG80L_001203"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS5023445.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="ACG80L_001204"
     CDS             complement(10708..11343)
                     /locus_tag="ACG80L_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS5023446.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="ACG80L_001205"
     CDS             complement(11409..12812)
                     /locus_tag="ACG80L_001205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS5023447.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="ACG80L_001206"
     CDS             complement(12799..13830)
                     /locus_tag="ACG80L_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5023448.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="ACG80L_001207"
     CDS             13861..14082
                     /locus_tag="ACG80L_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS5023449.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="ACG80L_001208"
     CDS             complement(14247..14984)
                     /locus_tag="ACG80L_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS5023450.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="ACG80L_001209"
     CDS             complement(15137..16477)
                     /locus_tag="ACG80L_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5023451.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="ACG80L_001210"
     CDS             complement(16714..18237)
                     /locus_tag="ACG80L_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5023452.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="ACG80L_001211"
     CDS             complement(18615..19226)
                     /locus_tag="ACG80L_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5023453.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="ACG80L_001212"
     CDS             complement(19556..20272)
                     /locus_tag="ACG80L_001212"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5023454.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="ACG80L_001213"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="ACG80L_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS5023455.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21601)
                     /locus_tag="ACG80L_001214"
     CDS             complement(20831..21601)
                     /locus_tag="ACG80L_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS5023456.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="ACG80L_001215"
     CDS             complement(21598..21969)
                     /locus_tag="ACG80L_001215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5023457.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="ACG80L_001216"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="ACG80L_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS5023458.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagc
    23041 cccattttca ttttaggtat taaa
//
LOCUS       DAZUMB010000037        22031 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000037 DAZUMB010000000
VERSION     DAZUMB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.037"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACG80L_001217"
     CDS             367..555
                     /locus_tag="ACG80L_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023459.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACG80L_001218"
     CDS             545..805
                     /locus_tag="ACG80L_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFS5023460.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACG80L_001219"
     CDS             846..1073
                     /locus_tag="ACG80L_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023461.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACG80L_001220"
     CDS             1138..1710
                     /locus_tag="ACG80L_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023462.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACG80L_001221"
     CDS             1711..2022
                     /locus_tag="ACG80L_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5023463.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACG80L_001222"
     CDS             2013..2273
                     /locus_tag="ACG80L_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023464.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACG80L_001223"
     CDS             2434..2793
                     /locus_tag="ACG80L_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023465.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="ACG80L_001224"
     CDS             2790..4433
                     /locus_tag="ACG80L_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5023466.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="ACG80L_001225"
     CDS             4468..5550
                     /locus_tag="ACG80L_001225"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFS5023467.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="ACG80L_001226"
     CDS             5594..6766
                     /locus_tag="ACG80L_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5023468.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="ACG80L_001227"
     CDS             6753..7457
                     /locus_tag="ACG80L_001227"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS5023469.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="ACG80L_001228"
     CDS             7462..8622
                     /locus_tag="ACG80L_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5023470.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="ACG80L_001229"
     CDS             8698..8976
                     /locus_tag="ACG80L_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFS5023471.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="ACG80L_001230"
     CDS             8957..9328
                     /locus_tag="ACG80L_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFS5023472.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="ACG80L_001231"
     CDS             9344..9748
                     /locus_tag="ACG80L_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023473.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="ACG80L_001232"
     CDS             9745..10155
                     /locus_tag="ACG80L_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023474.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="ACG80L_001233"
     CDS             10216..10803
                     /locus_tag="ACG80L_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS5023475.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="ACG80L_001234"
     CDS             10876..11343
                     /locus_tag="ACG80L_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFS5023476.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="ACG80L_001235"
     CDS             11389..11697
                     /locus_tag="ACG80L_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023477.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="ACG80L_001236"
     CDS             11892..18626
                     /locus_tag="ACG80L_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5023478.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18657..19502
                     /locus_tag="ACG80L_001237"
     CDS             18657..19502
                     /locus_tag="ACG80L_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFS5023479.1"
                     /translation="MNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSFREIGESIEL
                     SEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSLKSLSKTEID
                     SQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYTKNVWEKKGV
                     FKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFINMKDTQILE
                     VSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGHANVEIELYE
                     ERDLF"
     gene            19499..20548
                     /locus_tag="ACG80L_001238"
     CDS             19499..20548
                     /locus_tag="ACG80L_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023480.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="ACG80L_001239"
     CDS             20552..21376
                     /locus_tag="ACG80L_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023481.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="ACG80L_001240"
     CDS             21394..>22031
                     /locus_tag="ACG80L_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS5023482.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAZUMB010000038        21988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000038 DAZUMB010000000
VERSION     DAZUMB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.038"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG80L_001241"
     CDS             <1..395
                     /locus_tag="ACG80L_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5023483.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACG80L_001242"
     CDS             398..1147
                     /locus_tag="ACG80L_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5023484.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACG80L_001243"
     CDS             1159..1428
                     /locus_tag="ACG80L_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023485.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACG80L_001244"
     CDS             1433..1597
                     /locus_tag="ACG80L_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023486.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACG80L_001245"
     CDS             1590..1892
                     /locus_tag="ACG80L_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023487.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACG80L_001246"
     CDS             1889..2050
                     /locus_tag="ACG80L_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5023488.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACG80L_001247"
     CDS             2047..2352
                     /locus_tag="ACG80L_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023489.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACG80L_001248"
     CDS             2352..2708
                     /locus_tag="ACG80L_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS5023490.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACG80L_001249"
     CDS             2668..2913
                     /locus_tag="ACG80L_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023491.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACG80L_001250"
     CDS             2910..3329
                     /locus_tag="ACG80L_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5023492.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACG80L_001251"
     CDS             3326..3883
                     /locus_tag="ACG80L_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS5023493.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACG80L_001252"
     CDS             3880..4176
                     /locus_tag="ACG80L_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023494.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACG80L_001253"
     CDS             4253..4666
                     /locus_tag="ACG80L_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5023495.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACG80L_001254"
     CDS             5012..5386
                     /locus_tag="ACG80L_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023496.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACG80L_001255"
     CDS             5423..5590
                     /locus_tag="ACG80L_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023497.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACG80L_001256"
     CDS             5616..5960
                     /locus_tag="ACG80L_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5023498.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACG80L_001257"
     CDS             5965..6246
                     /locus_tag="ACG80L_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023499.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACG80L_001258"
     CDS             6349..6663
                     /locus_tag="ACG80L_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS5023500.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACG80L_001259"
     CDS             6641..8335
                     /locus_tag="ACG80L_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5023501.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACG80L_001260"
     CDS             8355..9533
                     /locus_tag="ACG80L_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5023502.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACG80L_001261"
     CDS             9496..10182
                     /locus_tag="ACG80L_001261"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS5023503.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACG80L_001262"
     CDS             10182..11342
                     /locus_tag="ACG80L_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5023504.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACG80L_001263"
     CDS             11352..12227
                     /locus_tag="ACG80L_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023505.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACG80L_001264"
     CDS             12224..12535
                     /locus_tag="ACG80L_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023506.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACG80L_001265"
     CDS             12525..12878
                     /locus_tag="ACG80L_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5023507.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACG80L_001266"
     CDS             12868..13269
                     /locus_tag="ACG80L_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023508.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACG80L_001267"
     CDS             13262..13666
                     /locus_tag="ACG80L_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023509.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACG80L_001268"
     CDS             13678..14286
                     /locus_tag="ACG80L_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5023510.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACG80L_001269"
     CDS             14306..14668
                     /locus_tag="ACG80L_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023511.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACG80L_001270"
     CDS             14671..14853
                     /locus_tag="ACG80L_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023512.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACG80L_001271"
     CDS             14870..18289
                     /locus_tag="ACG80L_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5023513.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACG80L_001272"
     CDS             18340..19077
                     /locus_tag="ACG80L_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5023514.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACG80L_001273"
     CDS             19087..21378
                     /locus_tag="ACG80L_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5023515.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACG80L_001274"
     CDS             21402..>21988
                     /locus_tag="ACG80L_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS5023516.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAZUMB010000039        20860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000039 DAZUMB010000000
VERSION     DAZUMB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.039"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            624..1313
                     /locus_tag="ACG80L_001275"
     CDS             624..1313
                     /locus_tag="ACG80L_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5023517.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1300..2508
                     /locus_tag="ACG80L_001276"
     CDS             1300..2508
                     /locus_tag="ACG80L_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5023518.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2802..4073
                     /gene="serS"
                     /locus_tag="ACG80L_001277"
     CDS             2802..4073
                     /gene="serS"
                     /locus_tag="ACG80L_001277"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS5023519.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4622..6106)
                     /gene="guaB"
                     /locus_tag="ACG80L_001278"
     CDS             complement(4622..6106)
                     /gene="guaB"
                     /locus_tag="ACG80L_001278"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS5023520.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6611..7306)
                     /locus_tag="ACG80L_001279"
     CDS             complement(6611..7306)
                     /locus_tag="ACG80L_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS5023521.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7396..8496)
                     /gene="ychF"
                     /locus_tag="ACG80L_001280"
     CDS             complement(7396..8496)
                     /gene="ychF"
                     /locus_tag="ACG80L_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS5023522.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8505..8696)
                     /locus_tag="ACG80L_001281"
     CDS             complement(8505..8696)
                     /locus_tag="ACG80L_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS5023523.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8717..9607)
                     /locus_tag="ACG80L_001282"
     CDS             complement(8717..9607)
                     /locus_tag="ACG80L_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS5023524.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9594..10361)
                     /locus_tag="ACG80L_001283"
     CDS             complement(9594..10361)
                     /locus_tag="ACG80L_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5023525.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10373..11086)
                     /gene="rsmG"
                     /locus_tag="ACG80L_001284"
     CDS             complement(10373..11086)
                     /gene="rsmG"
                     /locus_tag="ACG80L_001284"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS5023526.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11318..13219)
                     /gene="mnmG"
                     /locus_tag="ACG80L_001285"
     CDS             complement(11318..13219)
                     /gene="mnmG"
                     /locus_tag="ACG80L_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS5023527.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13236..14633)
                     /gene="mnmE"
                     /locus_tag="ACG80L_001286"
     CDS             complement(13236..14633)
                     /gene="mnmE"
                     /locus_tag="ACG80L_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS5023528.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14932..15315
                     /locus_tag="ACG80L_001287"
     CDS             14932..15315
                     /locus_tag="ACG80L_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023529.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15483..16241)
                     /gene="jag"
                     /locus_tag="ACG80L_001288"
     CDS             complement(15483..16241)
                     /gene="jag"
                     /locus_tag="ACG80L_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS5023530.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16259..17077)
                     /locus_tag="ACG80L_001289"
     CDS             complement(16259..17077)
                     /locus_tag="ACG80L_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS5023531.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17074..17430)
                     /gene="rnpA"
                     /locus_tag="ACG80L_001290"
     CDS             complement(17074..17430)
                     /gene="rnpA"
                     /locus_tag="ACG80L_001290"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5023532.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17548..17682)
                     /gene="rpmH"
                     /locus_tag="ACG80L_001291"
     CDS             complement(17548..17682)
                     /gene="rpmH"
                     /locus_tag="ACG80L_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS5023533.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18205..19539
                     /gene="dnaA"
                     /locus_tag="ACG80L_001292"
     CDS             18205..19539
                     /gene="dnaA"
                     /locus_tag="ACG80L_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS5023534.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19737..>20860
                     /gene="dnaN"
                     /locus_tag="ACG80L_001293"
     CDS             19737..>20860
                     /gene="dnaN"
                     /locus_tag="ACG80L_001293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS5023535.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRT"
BASE COUNT         5948 a         4257 c         3828 g         6827 t
ORIGIN      
        1 actttgttca gttttcaaag gtctacttca tcaggagtgt ttcccgattc gtttttggct
       61 gccgtagcaa cttttatatc atatcagaga gacaagtaat tgtcaactct tttttaaaag
      121 ttttttcgat ttcgtttttc gaaatcttcc agcaactcat ttatcataac ttatcgtttg
      181 ttatttgtca agaacttttt tgaagttttt ttcgatagaa tcaagcttcg caagtggttc
      241 ttttttaacg actcagttat cttatcatgt catttccaac atgtcaacaa cttatttttt
      301 attatttaat aagttattgg tgttcttttg aacaaataat aatatatcaa catcacatgt
      361 gaaagtcaat aagaaaatgc ttatttatca acatttctag cttacatgat cattggttag
      421 ttctggtcct ttttcgttcg tctttataac tccgagagac gtaaaatcct tttgttttca
      481 gtttaatgtt catgttcttg tcttattcac accaactaga tgtctcacct tctaaaattt
      541 tccttaacaa agtgctgctt ttatctattt ctgctttttt ttgctagaat aatagtaaca
      601 atatgactct ggaggattta tttatgaaaa ttttgattgc tgacgatgat aaagaaatcg
      661 ttgaattatt aagtatctat gttcacaatg aggggtatga agcagtaaag gcttatgatg
      721 ggaaagaagc attatctaaa atcagaacaa tccctgatat tgaattactt attttagata
      781 tcatgatgcc tgagatggat gggatgcaag tagtgaaaga gctacgaaaa gaatcccaaa
      841 taccaatcat tatgctgaca gcaaaaacta ctgatatgga taagatcaaa ggactggtcg
      901 ctggtgcaga tgattatgtg accaagccat ttaatccgtt agaagtaatg gctagagtaa
      961 agtctttatt acggcgtaca aaaatgcaga ttgccccaga tcagcctgat gtattagaag
     1021 taaatgcatt aatcatcaat aaagactctc atgaagttaa aacaatcgat gggaaagaaa
     1081 tccagttaac agctttggaa ttcggtatcc tctacttgct tgctagtcat cctaatcgag
     1141 tatttagcgc ggatgaaatt tttgagcgtg tttggaaaca agaaagtatt gtctctgcta
     1201 aaactgtcat ggttcacgtt agtcatttaa gagataagat tgaagaagca actggtggtg
     1261 aaaaagtcat tcaaaccgtt tggggagtgg gctataaaat tgacgcaaga tagtaaacga
     1321 gcaactgaaa aacgccgtcg gatcaatttg acttcaaaag aaatcagcga attgttagcg
     1381 gaaggaatca tcacgattat ccttttgctt ctattaaatg tttctattct ggttgttatc
     1441 agttcggtga tcaacagttc accttctttg acgaatgcga tttgggattc aaagaatatt
     1501 tttgcagaac gattgaacac tgatttgttc tggaacggcc ggaattttat tattcccttt
     1561 ttctttctat tagatattgg tgttttgtat tggcgcttga ttcgaagata tcgccaaatg
     1621 caattgcggc atatcatcag cgaacttcac tacatcgcaa acggaaatta tgatcatcgt
     1681 atcccttttg aactgagtgg tgacttaagc cgagtggtaa caagtatcaa tggattggtg
     1741 gatagtaccg tagcagcgat tgaagacgaa cgtaaaatcg aaaaatcaaa agatgaattg
     1801 atcacgaatg tcagtcatga tatccgtacc ccactcacgt ccatcattgg atacctagga
     1861 ctgatagaag atggccagta tcactcagaa gaagatcttt taaagtatac gcatactgct
     1921 tatatcaaag caaagcagat gaaatcttta gtggacgact tatttgaata cactaaggta
     1981 cgccagcctg ctgtcccggt gaatttttct gcctttgaca tgatccagtt gatcgaacag
     2041 ttagcagctg attttgagtt ggaagcttct aaaaaaaata tccaaatact tgttcaatca
     2101 aaagtagact cccttatcat ggatggcgat accgaaaaac ttgtacgtgt cttcaataat
     2161 cttttgacga atgcgttgaa atacggaaaa ggagcaacga agatcgtaat cgaagtagaa
     2221 cgtatcggtt cagaagttgt tgcaacagtc aaaaataacg gagcaatgat cccacaacag
     2281 gcaatcgata atctattcga ccgtttttat cgtgtagaag aatctcgttc acaagcaact
     2341 ggaggaactg gacttggttt agcaatcgct caaagcattg tggctttgca cggcggatac
     2401 atttatgcaa aatcagataa gcaatggact tctttcatca tgcaccttcc aatcaagaaa
     2461 aatgaaaaat tacctattga tacacaggaa gttattgacg aaagctgaaa tattcgctat
     2521 tcttattaca attattagaa taaatgaaaa acacagaacc cagtacactt tattcttttt
     2581 cagagagctg atggcaggtg taaatcagca aaggataatt tggaagttcc agttcgttta
     2641 cgtgcccttt cgaaataaga aaggtcggtt caacagccgt tatgtgttca acaagtgcag
     2701 ctgacgctga acttgggtgg taccgcgaat ttcagactga ttcgtcccaa gaataatggg
     2761 atggatcagt ctttttttat tttcaaaaag gaggaaatca tatgttagat gtaaaaatga
     2821 tgcgacaaaa tattgaagaa gtacaagaaa aattgatgac tcggggcgta aaaaaagaaa
     2881 cattagctga attcatggaa ctagatgaaa atcgtcgtaa gctactagtc aaaagtgaag
     2941 aattaaaaaa atatcgaaat gatgtttctg ctgagatcgc ccaattgaag cgtgcaaaag
     3001 aagatgcttc tgctaaaatc gctgaaatga aagaagtcgg tggcaacatc aaagcattgg
     3061 atactgaaat tgctgaagtg gatgaaaaat tgaaacaaat tgcaactact ttgccaaacc
     3121 ttccccatga ttctgtgcca gttggaacag atgaagagga caatgtagaa atccgtcgtt
     3181 ggagcgaacc acgtacattt gcttttgaac caaaaccaca ctgggaaatt gcagaaaatc
     3241 ttgatatttt agattttgaa cgtggcgcaa aagtagccgg tagccgtttt gtctattaca
     3301 aaggattagg tgctcgatta gaacgtgctg tctacaactt catgcttgat cagcatattt
     3361 acgaacacgg ctatactgaa atgatcactc cttatatcgt caacagtaaa gcgatgtttg
     3421 gtacgggaca atttccaaaa ttcaaagaag atgttttcca attggagaat actgatctaa
     3481 cattaatccc aacagctgaa gtaccattaa ctaactatta taatgatgaa attttagatg
     3541 gtgcagattt acctatttat ttcactgcac taagccctgc ttttcgttct gaagccggaa
     3601 gtgctggtcg ggatacacga ggactgattc gtcttcacca attcaataaa gtagaaatgg
     3661 tcaaattttc ggatgtagag cattcttacg aagaattgga aaaaatgaca gctgatgcag
     3721 aagatatctt gaaaaaattg catttgcctt atcgtgtgat ggccttatca actggagaca
     3781 tgggcttttc tgcagctaaa acttatgact tagaagtatg gatacctgca caagatactt
     3841 atcgtgaaat cagttcttgt tctaactgtg aagatttcca agcacgccgc gcaatgatcc
     3901 gttatcgcga taatgatgga aaaatccact atactcatac tctgaatggt tcaggacttg
     3961 cagttggccg tacagtagct gccattttgg aaaactacca aaatgaagat ggttctgtca
     4021 cagttccaga agcgctcgtt ccttatatgg ggaatctgaa agttattaaa taaagaggct
     4081 gtgaaaaagc cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     4141 ttttgcataa attggactta ataccgagaa actggctttt gaacaccatt tatcgagagg
     4201 ttgtgaaatc agcgaattaa cctgagtatt agagaaaaaa atggaaaaat agtccctcat
     4261 attttaccgt ttgttgaact aataccgagg gtcagctgat tgaacaccgt ttagtggaga
     4321 ggtcgtaagc ctacccccca gctcctagtt acaaggacaa attcaaaaaa cagctcttca
     4381 tattttatga atgttgtact tgtgattcgg aggtggcaag ggaacactgt ttattcaaaa
     4441 agaggctggg acagaagcgt ttaactccaa gaaataagaa gaaattcacg aaaattgctt
     4501 ttcaaatttt tgtgaatttc ttcttatttc cgaaggagtt gcttctactt ccgccgttta
     4561 tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct ctttaatgtt
     4621 tttaagattc aactgaatag tttggtgctt cttttgtgat ttgtacatca tgagggtgtg
     4681 attcttttaa tccactgccg ctcatttgga taaattgcgc ttcatcacgt aattgctgta
     4741 gattggctgc cccaacataa cccattcctg aacgcaaacc gccgatcatt tggaaaataa
     4801 tatctgcaac acttccttta taagcaaccc gaccttcgat tccttctggt actaatttat
     4861 ttgcttcatt gacactacct tggaagtaac ggtcttttga acctttttcc atcgcgccta
     4921 atgagcccat acctcggtat gttttgaatc gacgtccttg gaagatttcg aattcacctg
     4981 gtgattcatc tgtccctgcc aacatacttc ctagcatcac tgcatgtccg ccggcagcta
     5041 atgctttgac gatgtcacca gaatatttga tccctccgtc tgcaataatc gctttgccat
     5101 attgacgagc aactgatgct gcatcataaa tagctgtgag ctgaggtaca cctacaccgg
     5161 ctaccacacg tgtcgtacaa atcgatcctg gtccgatacc aacttttacg acatctacac
     5221 ctgcatcata taaagctttt gtcgcttcag ccgtcgcaac gtttcctgca atcaatgttg
     5281 catctgcaaa agttgaacgg atttcttgaa tttttcggat cacacctgca ctatgtccat
     5341 gagctgtatc gatgatgatt gcatctgccc ctgcttctaa taaagcattc gcacgctcaa
     5401 atgtatcgct tgttacacca acggctgctg ctacaagcaa tcgaccatgt tcatcttttg
     5461 ctgcatttgg aaattcgatc actttctcga tgtctttgat cgtgatcaat ccgcttaaac
     5521 gaccttcgtt atctacgatt ggtaattttt cgattttatg tttttgcaaa atcttttctg
     5581 catcttttag agaggtacct actggggcag tcactaagtt gtcttttgtc atcacatcag
     5641 caatcggcat gtgataatct gttacaaaac gcatgtcgcg gtttgtaatt atccctacca
     5701 attttctgtt ttcaagtgtt tcaacgattg gcacaccact gatacgatat ttgctcatca
     5761 attcttctgc gtctgctaca agatgctgtg gcgttaagaa gaacggatca atgattacgc
     5821 cactttcaga acgtttgact tttcttactt catccgcttg ctggctgatc gtcatgtttt
     5881 tatggatcac acctagtccg ccttgtcttg ccatagcaat ggccatcttg ctgtcggtaa
     5941 cagtatccat acttgcactg atgattggaa tattcaactt aatattttta gctaattgta
     6001 cgctcatatc cacttcgttt ggtaacacgt gactttctgc tggtatcaat aagacatcat
     6061 caaaagtaaa tccttttttc gtgaactttg tttcccagtt ggacattttt agtaccactc
     6121 ctctaattta gtttatctat taaaataatt gattttttca tttaagtcaa tgatctactt
     6181 ggaatcaagt tagctttctc atagaatacc aatataaagc gtttacatct gtgtatatta
     6241 aaagagattg taaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
     6301 ttctcatgtt tttgaattgg ttgggtttat taccgcagat gcaaactttt gaacaccgtt
     6361 tagtggagag gctgtgaaaa agctgcttag tttcgagtag taagaaaaat tttgtgaaaa
     6421 tggctttcca catttttgca taaattggac ttaataccga gaaactggct tttgaacacc
     6481 gtttatcgta aaaagggact gtgacaagac ttttgtcaca gtcccttact attatttaac
     6541 tatggataaa caaaatgagg ttctttacta cccctagata tctagcaaaa tagctgatct
     6601 ttttgctagt ttatcttctt gggacggata aacttccaat aatcccgtat tttttcttca
     6661 agtagtaacg gattgctaaa gcagttgccc cgataatgat aactactact ggatctaata
     6721 ctggattgat aactggtggc aatagtgttg tcgcagaaaa cagtacgacc caaagaagtg
     6781 tcgttccacc taggataagc aatgtcttac ccattcctgg tttcttgctt cgatctgctc
     6841 cgggatattc atattgatag atgtatttgt acatcatata aaagacccag ccaccgattg
     6901 cagaacctag aacgagtgta gaaaagccat aagctggcgt tgttccacgt gaaaacaatc
     6961 cggtaatcgc aatcatgata cttaatagac caagcaacag caacgtatta tctaaccaca
     7021 tcaataaagg ggtggatgaa ttttttactt ctgctggctt attgatgatt gcttccgttc
     7081 tttcagagac tgtcccaaac aattgacggg ctgttcttcc gcctttttgt tctgcaacta
     7141 aagtaggcag aatgtcatga agggctttta ctttttcttc ttcagacaaa ttcgctgctt
     7201 ctaaagattt tttcaaatca aaaatgtact gttgatttct tttggttaat tttgtttcta
     7261 aactattgtt ttcttttacg attgctcgta attgttctgg ttccattatc taatagtcct
     7321 ccttagccgt tccttttctc aacggtcgtc acaaaagaca ttctaccata ttctacagct
     7381 agtttgatag cccttttaca cattaaagcg gaataacatt acgtcgccgt cttgtacgac
     7441 ataatctttt ccttctaatc gaacacgtcc agcttctttt gctgcatgca tgtttccgta
     7501 atgatccaag tcttcaaaag agacagtttc tgcacggata aatccgcgtt cgaaatctga
     7561 atggatgatt cctgctgctt gtggtgcttt gattcctttt ttgaatgtcc aagcacgtac
     7621 ttcttgttcc ccagcagtaa agtaagtagc taagcctaac aaatcataag cagcacggat
     7681 caactgatcc aaacctgatt cttcgattcc taaagcttct aaaaattccg ctttgtcttc
     7741 ttcatctaat tctgcgattt cttcttctgc acgagcactg accacgatca cttctgcatt
     7801 ttcttctgca gcaaagttgc gcacttcttg tacataatga ttcgcgtctg catctgctac
     7861 gtcatcttct gagacattgg ctacataaag aattggtttt gtcgtcaata aaaatagtga
     7921 tttaacgatt ttttgttctt cttcagtaaa ttcgatcgtt cgggcagatt ttccttcttc
     7981 cagtacaggt ttgattttat ccagtacagc tagttctgcc actgcatctt tatctttggt
     8041 tttagcaact tttgctacac gagtatagcg tttgttgata gattctaagt ctgctaacac
     8101 tagttccaag ttgatcgtat caatatcttc tagtggatca acacgtcctt ctacgtgagt
     8161 aatattatca tcatcaaaac agcgaacaac gtgacaaata gcgtctactt ggcggatatg
     8221 gcttaagaat tgatttccca aaccttctcc tttacttgcg ccttttacga ttccagcaat
     8281 atctgtaaat tcaaaagtcg tcggaacagt ttttttcgga tgtacaagtt ctgttaagcg
     8341 ttgcaaacgc cagtcaggta cttctaccat ccctacgttg gggtcgatcg tcgcaaaggg
     8401 atagtttgct gcttctgctc ctgcttttgt gatcgcatta aacaaagttg attttcctac
     8461 gttgggcaac ccaacaattc cagcagtcaa tgccataaat ttattcactc tttctctgca
     8521 tttttttcaa ttactttttt taatttcttt tcaaaatctc gacgggtcat catgacgata
     8581 tgtccgcaat tcatacatct gattttgata tctgcgccca ttcgaatgac ttcccagcga
     8641 ttcgtctggc aagcgtgagg ttttttcatt tcaacgatat cgtgcaagtc gtacataaaa
     8701 atagcctcct ttttgtttat tcttcatcaa attgaatatt caagatatca agaattcgag
     8761 tcaaatcttt ttgagaaaga tattcaatct cgatcttacc tttcccattt ttttcttgga
     8821 tagctacact tgtaccaaat ttatccatca aacgatcttc gctttctcgg atataatatg
     8881 gtttttcctt tgctagcctt gggatttttt tcttttcttt tccatcattt tgatttaact
     8941 cagaaacaag ctgttccagt tggcggacag ttaaattttc agttactgca cgattggcta
     9001 atttcaagat caatgtttta ttttttaaac ctaacagcgt acgggcttgt cccatagata
     9061 aacgctgatc ttgtaccatt tccttgacaa gatccggtaa agaaagcaag cgcaaataat
     9121 tggcgatata aggtctgctc tttcctaaac gactagctac ttctgcttgt gttagtttca
     9181 ggtttttcat cagcatctca tatgcttctg cttcttctaa cggattcaga tcttctcgct
     9241 gcaagttttc cagcactgct acttgcatca tcgcttcttc gtcaaagtct ctaacgatag
     9301 cagggatcgt ttctttttca gctagtttgg acgctcgaaa acgtcgctcc cctgcaataa
     9361 tctcatatcc ttttactgcg gaacggcgta cgatgatggg ttgaaagaca cctgattgtt
     9421 caatagaatt agctagttcc tgcaaagata cttcttcaaa tgttttacgg ggctgatacg
     9481 gatttggtcg cagctcattc aatggtagct ggacaacggt atcatctttt acatccaccg
     9541 tctctaagtt ttctaaatct tgaaacagtg catcgattcc tcggcctaat cctttacttt
     9601 tcttcacgac ttaccacttc ctttgccaat gcttggtaga cttcagctcc tcgagaacgt
     9661 ggatcgtagt cgatgatcgg ttttccatga ctaggtgctt ctgataaacg aatatttctt
     9721 ggaatgatcg tttcgtaaac tttttcgcgg aagtatttac gtacttcttc tacgacttca
     9781 tttcccagat ttgttcgtgc atcatacatt gtcagtaaga ctccctcgat ctctaattct
     9841 ggattaaagt gtttttggac taaacgaacc gtattcagta attggcttaa tccttccaat
     9901 gcgtaatact cacactgaac aggtatcaaa atcgaatcac ttgctgtaaa tgaattgatt
     9961 gtcagatggc caagagaagg cgggcaatcg atcaaaatat aatcatactg actgcttact
    10021 tctgctaaag atccttttaa tcttgattct cttgccatca ttgacgtcaa ttcgatctct
    10081 gctcctgcta attggagagt cgcaggcaca atactcaaat tttcgtgttc agtgatcaaa
    10141 gttgcctcat caattggcaa ttcattgact agaacatcat agatgtctct tgtcacatca
    10201 ggttttctga tcccgacacc actggtagca tttccttgtg catccatatc cactagcaat
    10261 acttttttgc caaggctagc aagacaagca cctagattca ccgtcgtcgt tgttttgccg
    10321 acgccacctt tttggttcgc aacagatatt atttgtgcca tctcttctcc tcctatttga
    10381 ttggctgctt attaggcagt ccgggctttc ttgggtattt ctttggggtt ggtttctttt
    10441 tttgaataac gatgatatgc cgttgatcag agacattcgg caaggtaacc gcctcttctt
    10501 tgaggaattt accacctaat aaagcaatcg cttgttttgc ttctgtcaat tcctcttcac
    10561 tttttgctgc tttcaatgca taaaaatagc cttcttgttt agccaagggc aaacacaatt
    10621 ccgctaacac attcaaacga gcaaccgcac gagcagtgac aaggtcaaat gatgcacgaa
    10681 agtgaggatt ttgcccaaat gtttctgccc ggtcatgata taaggaagca tcgattccta
    10741 ataatcgggt taactcagat aagaaggtta ttcgtttatt cagagaatca acgatcgtta
    10801 tctgcagttc tggaaaggca atttttaaag gtatgctagg aaaccctgcc cccgaaccaa
    10861 catcgcataa agataaaggt tgcgtaaagt ctacttcaaa tgctaatgta agtgaatcgt
    10921 aaaaatgctt cagatatacg tctttttcat ccgtaatagc tgtaaggttc atcttttcgt
    10981 tccattcgac taatgtccga taataaatag cgaattgatt catttgatga tcggtaagct
    11041 ctatcccttt ttctgccaag gattggcgaa attcttctgg tatcataaat attcctttca
    11101 ttgtgaaaca gcaattgttt cacagattct ctccctactt taacgcaaaa tctctagaca
    11161 tgcaatcctt ccatatggat tcttaaaaat ttgaaaagta agaaaagccg atagactttt
    11221 tctttctagt atgactcata aaatatacac agaagagaga actagctttg agcaatttct
    11281 gcaaaaacta gttctctctt ctatttatta ttacgtatta cgctggttca ctgactttcg
    11341 caatcttccc ttgttcgata tagaccatta ggatacttag gtctgcaggg tttacgccac
    11401 tgattcggct agcttgggca attgtttctg gttggatttt ctgcaatttt tgttttgctt
    11461 ctgttgctaa tccgttgatt gctgcgtagt cgatgttttc tggaatcttt ttcgcttcca
    11521 tgcgttttaa tttttctact ttttccatgg cttttttgat gtagccttca tatttgattt
    11581 ggatttctac ttgttcgatt tcttttcgtg tcaattcttc attttctgga ataaaccgta
    11641 gaagttctgc ataagtaatc tcaggacgtc ttaaaaagtc gcttgctagt acaccatctt
    11701 ttaatggtgc cgcattaatc gttgctaaaa agtcttggac ttcttctgtt ggtttgattc
    11761 gtacattcat caaacgtttg atttcttgtt caactgcttc ttttttcttc aaataattct
    11821 tgtattgatc ttcttttact aagcctacac ggtgacccat ttctgttaat cgcaaatctg
    11881 cattatcatg acgtaaaagc agacggtatt cagcacgtga agtcagcaaa cgataaggtt
    11941 cgttcgttcc ttttgttacc aaatcatcga tcatgacacc gatgtaaccg tcacttcgtt
    12001 tgatcactaa aggttctttt ccctgaactt tcaaagctgc attgataccg gcaatcagcc
    12061 cttgtccagc cgcttcttca taaccgcttg taccatttgt ttgtccagct gtataaagat
    12121 tttcaacgac tttagtttct aatgtcggac ggagctggtg tggctctact acgtcatatt
    12181 cgatagcata tcctgtacgc atcatttcta ctttttctaa tccagcaata gaatgaagca
    12241 tgttgacttg aacatcctct ggtaaagaag tagataaacc ttgaacatag acttcttccg
    12301 tatttaagcc ttctggttcc aaaaataatt gatgacgtgg tttgtctgca aaacgcacga
    12361 tcttatcttc gatggatgga caatagcgag cgcctacccc ttcaacgatc cctgtaaaca
    12421 ttggggcacg atgtaagttg tctcgaataa tttggtgagt cgtctcacct gtataggtca
    12481 gccagcaaga ttcctgatct agtttatagg cactatctgg tgtgctgaag ctaaaatgat
    12541 tcggctcttt gtcaccaggc tgctcttctg taacagaata gtcgatcgta cttgatttta
    12601 ctcgaggcgg tgttcctgtt ttgaaccgtt cgatagcaaa gccaagttct tttagatgat
    12661 tcgctagtcc aatagaaggt tgagagttat tcggacctga tgaatatttc aattcaccga
    12721 taatgatttc tccgcgaagt gctgttccgg ctgtaataac aactgctttg gcccggtagc
    12781 tcgctcctgt ggacgtcact actccatgac aaacaccatc ttcgacgatc agttcttcga
    12841 cgattccttg tcttagcgtc aaatttggtt ctttttcgat cgtatgtttc atttctgttg
    12901 aataagcatg tttgtctgct tgtgctcgca atgctcggac agctgggcct tttcctgtgt
    12961 tcaacatacg catttggata taggttttat cgatattctt acccatttct ccgcctaacg
    13021 catcgatttc tcgaacgaca acgcctttag ctggtccgcc tacagaagga ttacatggca
    13081 tgaatgctac catgtccaaa tttattgtca gtaataatgt ttttgatccc atacgtgcag
    13141 cagcaagtgc agcttctgaa ccggcgtgtc ctgcgccaac aacgatcacg tcatacgcat
    13201 ctgcttcata atgttccaat cgtttgttcc tccttttatt ttcctaaaca aaattgactg
    13261 aataattgag tgattagctc atcttgtaca ctgtcgccga caacttctcc tagatagtcc
    13321 cagcaacgcg tcatatctat ttgaacgaga tccacaggca ttcctgaatc aatacctgca
    13381 atgacttctt caagtgagag ggaagctttt tctaacaacg cgatatggcg ggtattagaa
    13441 acataagtag cgtctctttc tcctgtttct ccgctgaaga ataaatcaga aatagctgat
    13501 tccaaacgat ctagtccgtc gtttttagct acggaaattg aaaaaactgg ttcattttcg
    13561 atcaatttct tcaatttttc ttgttccagc tgtgctggca aatctgtttt atttagaaga
    13621 atgatccgtt tcaatccact tgtcgcttcc aataattgtt catcttctgc tgtcagtggt
    13681 tcgctttggt ttagaacaag taagatcaag tctgcttctg ctaatgcttt tcggcttctt
    13741 tcgaccccga tccgttccac cacatcttct gtttcccgaa ttccagctgt atcgattagc
    13801 ttgagcggta ccccccgaac attgacatac tcttcgatta catctcgagt cgttcctgca
    13861 atatccgtaa cgattgcttt ttcttctctt aacaaatggt ttaacaaact tgatttgccg
    13921 acatttggcc ggccgatgat tgccgtactt aacccttccc gcagaacctt cccttgcttg
    13981 gatgtagcaa gtagcgcttg gattctttgt tggatcatct gcgctttttc aagcaataac
    14041 ttcgtagtca attcttcgac atcatcatat tctggataat caatatttac ttctacttgt
    14101 gctaatgtct ctaaaatctc ttgtctcaat gagcggatca aagcagacag attgccgtct
    14161 aactgattaa gagctagtcc cattgctttg tctgtttttg cccgaatcaa atccatcaca
    14221 gcttctgcct gtgaaaggtc aacacgacca ttcaagaatg ctcgttttgt gaattcgcct
    14281 ggctcagcta atcttgcacc ttctcttaac aatagctgca aaatttgatt gactacaacg
    14341 atcccaccgt gacaattgat ttctactaca tcttcccgcg taaatgtctt tggtgctctc
    14401 atcacggaga ccatgacttc atcgacaagc tgattgctct tgggatcaac gatatgtcca
    14461 taatggatcg tatgacttgg tacttctgat aatcgtttgt ttccagattg ataaacttta
    14521 tctgctagtt gcacagcttg atcgccactt aaacgaacga tactgattgc accttctcca
    14581 ggtggggtag aaattgccgc aatcgtatca aattctaaag taatttcagc cattttttca
    14641 cttccttttt cattttgcgc acaaaaaaag tgcccactcc accctttatg cacaataaac
    14701 ggtgaacttc gcactttgac tgcttgttct tgattaaatt ctggtcatag attatcacac
    14761 ttgctataag gaatcaagtg tttaaaacaa ttagatgaag ttgagcctgt tctgcttcca
    14821 ttaccttata tttaattgtc cttggatgtc tctttgcgtt ctttggttta ttcgaagaga
    14881 agatactgta taaacaaggc tttatctttc ttaaaaaaaa cggtacacta aatggtagcg
    14941 ctatctaaac tatacacttt tgaaaggaga aaatatgtga gcccgttcag atctgctgca
    15001 ttggaagaag aaagtgaccc tcggatcaac gatgtcgttt tatctacagg gaatatcaac
    15061 cagaaatata tcgtccgcga tatcgttttt gttgtggaat gcttagaagc tgatttgttt
    15121 gacccatctt ttcccaaaga tgaccttttc ttaacaacga agaggaaatt aaagacgaag
    15181 gtacttgaat atggtgcgaa tgcagtaatc aactgtcatt ttgagtatca gcaaaaaaaa
    15241 cgcgacggaa agtattttct ggaacttttt gcttatggaa cagtgatcca atttatccaa
    15301 acaacgattg gttaaatgaa acagacaaaa aaagcggatc aaaactgaag acagtttcga
    15361 tccgcttttt tctatcaaag ttagtgacta cttcatcgta tactctgata agaatgattt
    15421 ctagtaaaat atacttggtt tgaaaaactt tgtcaacgtg atttattcaa aaagtaatgg
    15481 aatcaataat attttttagc aggttctact actaaataac gataaggttc ctcaccttcc
    15541 gaatgtgttt ttacatgttc atctttgctt aatgcagcat ggatttgttt acgttcaaat
    15601 gcaggcattg gttctaagaa aacaggctgc cctgtccgtt ttactttttc agccgtgcga
    15661 tcagctaaac gctgaatgat ctcttggcgc ttttcacggt aattgcctac attgactact
    15721 acagacagct tatttcttgc taaacgatgg atgaagactt gtgccagata ttgcaatgca
    15781 ttcagtgttt ttccatgttt cccaatcagt atgccttgct tttgtgtttc taaatggaaa
    15841 acaaccatat tatttgattc tctttccatt ttgacaagag ctggagcatc aagctgtttt
    15901 gaaatttcag taagataaac tgccaattcc gttaaagctg cttcatcacc tagctcactt
    15961 acttcttctg ttttttcttc aagaatcgtt tcgttgacta cctcgatctc agctgttggt
    16021 tcttcttcca aaatttcttc tacagtctgt tctactgttt cagaaacttt ttcgctgata
    16081 cttggctgga tcgaaagccg tgcattcttt cgacccattc ctaggaaacc tttttttcct
    16141 tcttccagta tttcgatttc gacttggtct ttggccaaac ccaaatcagc cagacctagt
    16201 tggacagctt cgtctattgt tgcagcttca taaatcggca ttagcattac ctcccctatt
    16261 atttccgttt tttcttagga ttcattgctt tcttaagagc acgttctctt tcgcggattc
    16321 tccgtgcttc ttcttcacgc tcttttcgaa ttttgaaagg gttgttgatc agcagcgttt
    16381 gtacaacttg gaaggcattg gaaactaccc agtataatga aagaccgctg gctaaattga
    16441 cacccatcac taaaatcatt acaggcatca cgaaattcat gatcttcatt gatgcattcg
    16501 attcaatttg actcatgcta gataaatacg tacttgcaaa agtaaagatt gctgctaaaa
    16561 tcggcaaaat aaatgttgga tccgcgttcc ctaagttcaa ccataagaaa tgcccttgtt
    16621 tcaactctgg tacacgtgaa atagattgcc ataacgccat caagattggc atttgtacta
    16681 ataaaggaag acaaccagca tatggattga cgccattttc tgcatataaa cgctgctgct
    16741 cttctcttaa cttactttgt gtttccggat ctcttgaact atattgttgc tgcagtgctt
    16801 ttaatttcgg ttgtaattct tgcgttttac gcatgctctt tgtttggaaa tgcatcaacg
    16861 gcaataggat cacgcggatg atcagtgtaa ataaaatgat ccctatccct gcattaccaa
    16921 atgacaacgc cttgatcgcc tcagcaaagt aataaacaat ataacgatcc caaattcctg
    16981 tactctgtgc gctgacttcc ccagttccgc aaccggataa gacgatgacc aaactaacga
    17041 taccggccat caaaagtaaa cgtttgtatt tcttcactta ctcttttcct ctctgtcgat
    17101 tatttttgca agttttaaga cgtggactaa attggagcga acctcatcat aagtcaaccc
    17161 cttcacactt ggtctagcaa tcacaataaa atccaactcc ggatcaattt cttcttttat
    17221 agaataaact gctgctcgga tttttcgttt gacttcattg cggtttaccg cgttgccgac
    17281 cttttttcct acagataaac caactcgaaa atgaggctgt cccggttttt ccaaacggta
    17341 aacgacgaat ttgcggttag caaaagaatc tcccgtatgg aacacggctt gaaactctcg
    17401 ttcttttttt actcgatacg cttttctcat tagttttctt tccttttcta tctctcactt
    17461 acgctcataa tcataccata attacgattg tcctttcatc atttccctaa aaaagagtat
    17521 aaaaaaaacc actgaaaccc cagtggccta agctgaaaga acttttcttc ctttgcgacg
    17581 acggctagct aacacacgac gtccattttt agtactcata cgtttacgga atccgtgaac
    17641 tttttgacgt ttacgtttat ttggttggta tgttcttttc atatattcca cctccaaaat
    17701 gttcttatct attttataaa cacagacata ctttgatagt ataacgacaa agtagtgatt
    17761 ttgtcaatat aaaatagaag atcgattgtt aaaacttatc cacaagatta tttttttcta
    17821 tgggaatcct atgtggaaaa agttatgcac agggattatc cacagataat aaaaggctgg
    17881 ttttacacag aaaacacacg tgtaaaaaaa gtaatccaca atcaggcgat ttcctgtgta
    17941 ttccgtctcc taactattgg tatatctgta ttttttatcc acagacgcaa gaggaaatct
    18001 attggttttt acgagttttc cacctgtgca ttagtggtgt ggaaaacttt ttttgacaac
    18061 tgtgtttttc tcttttttct ttttgtcgat tgtgtggata attgtaatta aaaaattttt
    18121 ttcttcggtt tctgtggaaa actattgaaa aaaatgctaa attagtacta gttattcact
    18181 ttttaatgaa ggaggaggcc ttatatggta tccctcgatg ctttatggaa tgaattaaaa
    18241 gcaacatacc aaaaggattt atcgcctgct agctataata catggatcga aacagcgaac
    18301 cctcgaaccc ttgatcaaaa ccagctcgtt gttgaggttc ctagcaagat tcataaagaa
    18361 tattgggaaa aaaacctggc gacaaaaatc gttgaagtcg gttatatgtt atcaggaaat
    18421 gagattatcc ctcgctttat tactggcgaa gaagcagagc aagaagaagt tatagaagaa
    18481 aaaaatccaa aagttgtggc acctagtccg ctaaaaaaag ccatgctgaa ccctaaatat
    18541 acctttgata cgtttgtcat tggtaaagga aaccagatgg ctcatgcagc agcgctcgtt
    18601 gtagcagaag atccaggttc tatttataac ccgctcttct tttatggagg cgtgggactt
    18661 ggtaagactc acttgatgca tgcaattggt catcaaatgc tgcaaagtca gcctaatgcc
    18721 aaagtaaaat atgtaagcag tgaaactttt gctaatgatt tcatcaattc gatccaaaac
    18781 aaaacagcag aagagttccg tcaagaatat cgaaatgttg atttactgct agtggatgac
    18841 attcaatttt ttgcagaaaa agaagctacg caagaagagt ttttccatac tttcaatgcc
    18901 ttgtacaatg aagggaaaca aatcgtacta accagtgatc ggttgccaaa cgagattccg
    18961 aaactgcaag aacgccttgt ttctcgcttt gcatgggggc tgtctgttga tattacaccg
    19021 ccggatcttg aaacaagaac agcgatttta cgtaaaaaag cggcggctga acgtctagag
    19081 atcccagatg acacactaag ctacatcgca ggacaaattg attctaacat tcgtgaatta
    19141 gaaggtgcgc ttgttcgagt gcaagctttt gctgcgatga atagtgaaga tatttcgact
    19201 agtctagctg cagacgcact gaaaacgtta aaatcaggaa aaggacatcc tcaattgtca
    19261 atcctgcaga tccaagaaga agtggcaaag tattatcaca tccaattgaa agatctcaaa
    19321 ggaaagaaac gcgtcaaatc tattgttgtt cctagacaaa tcgctatgta tttggcacgt
    19381 gagctcacag ataattcttt accgaaaatc ggagctgaat tcggtggcaa ggatcataca
    19441 acggttatcc atgctcatga aaaaatccag cagctgatgg attctagtgt cagtatgcaa
    19501 aatgaagtat cagaaatcaa aaacttttta ctaaattaat ttgtcgattc tatcaactga
    19561 aaatagcatg tggataaaag aaaaaaacgt tcaaaagtta tacacacttt ttccacagct
    19621 gttttttcgt ttctctctta cgttttttga gttttccaca gaatcaacat gccctattac
    19681 ttttattatt ctttttaact aataataaat aaacaaacat tcaaaggaga tacatcatga
    19741 aagttacttt aaaccgagct agctttatgc aggaattgca aactgttcaa cgagctattt
    19801 caagcaaaac cacgatccct attttgacag gtgtaaaaat cacactgaca caagaaggtt
    19861 tgactttgac ggggagcaac gctgatatat caattgaaac ttttttgtct gttgaaaacg
    19921 aaaaagcaaa tatgcaaatc gaatctactg gttccattgt tttacaagca cgtttcttta
    19981 gcgaaatcat tcggagactt cctgaagaaa catttacttt agaagtttta gaaaataaac
    20041 aagtagcgat cacttctgga aaagcgaatt ttatcgtaaa tggattagat gcagataact
    20101 atcctcatct tcctgttgtc gaaagccata accagatgaa attacctgta cacgtattga
    20161 ctaaactaat caacgaaaca gtttttgctg tctcacaaca tgagagtcgt ccaatcttga
    20221 caggtgtgca tttcatttta tctgataatt ctttattagc tgtagctact gattctcacc
    20281 gtctaagtca acgcgtgatt ccagtagaac aagcggctga tcattttgat attgttattc
    20341 ctggaaaaag tttgatcgaa ttatctcgct cattaacaaa tgaagaagaa atcgtcgaaa
    20401 tcagcattat ggaaaaccaa gtgttgttca aaacagaaac gatgtatttc tattcccgtt
    20461 tgctagaagg aaactatcca gataccaatc gtttgattcc ttcaagtttt aatacagagg
    20521 ttgaattttc tgttcctagc tttttagcgg cgatcgaacg ggcttcttta ctttctcatg
    20581 aaggccgtaa caacatcgtt cgtttatcta ttcgtccaga tgcggttgtt ttatacggaa
    20641 attcgccaga aatcgggaaa gtagaagaaa gcttgagtta tacagcaagc agtggcgatc
    20701 cattagatat ttctttcaat ccggattata tgaaagcagc gttgcgtgca tttggagata
    20761 tgagcatcaa agtgaaattt atctctgcta ttcgtccatt tacattggaa ccaacggaag
    20821 atggcgttca atttattcag ctgattactc ctgtacgtac
//
LOCUS       DAZUMB010000040        19968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000040 DAZUMB010000000
VERSION     DAZUMB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.040"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..344
                     /locus_tag="ACG80L_001294"
     CDS             48..344
                     /locus_tag="ACG80L_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS5023536.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(988..1941)
                     /locus_tag="ACG80L_001295"
     CDS             complement(988..1941)
                     /locus_tag="ACG80L_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5023537.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1964..2719)
                     /locus_tag="ACG80L_001296"
     CDS             complement(1964..2719)
                     /locus_tag="ACG80L_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023538.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2716..3675)
                     /locus_tag="ACG80L_001297"
     CDS             complement(2716..3675)
                     /locus_tag="ACG80L_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5023539.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3668..4618)
                     /locus_tag="ACG80L_001298"
     CDS             complement(3668..4618)
                     /locus_tag="ACG80L_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5023540.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4768..5220
                     /locus_tag="ACG80L_001299"
     CDS             4768..5220
                     /locus_tag="ACG80L_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS5023541.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5309..7576)
                     /gene="gshAB"
                     /locus_tag="ACG80L_001300"
     CDS             complement(5309..7576)
                     /gene="gshAB"
                     /locus_tag="ACG80L_001300"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS5023542.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7763..8761)
                     /gene="ftsY"
                     /locus_tag="ACG80L_001301"
     CDS             complement(7763..8761)
                     /gene="ftsY"
                     /locus_tag="ACG80L_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS5023543.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8772..9581)
                     /locus_tag="ACG80L_001302"
     CDS             complement(8772..9581)
                     /locus_tag="ACG80L_001302"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5023544.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9590..13171)
                     /gene="smc"
                     /locus_tag="ACG80L_001303"
     CDS             complement(9590..13171)
                     /gene="smc"
                     /locus_tag="ACG80L_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS5023545.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13191..13877)
                     /gene="rnc"
                     /locus_tag="ACG80L_001304"
     CDS             complement(13191..13877)
                     /gene="rnc"
                     /locus_tag="ACG80L_001304"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS5023546.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14225..16006)
                     /locus_tag="ACG80L_001305"
     CDS             complement(14225..16006)
                     /locus_tag="ACG80L_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5023547.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16032..16946)
                     /locus_tag="ACG80L_001306"
     CDS             complement(16032..16946)
                     /locus_tag="ACG80L_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023548.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16973..17935)
                     /gene="opp4B"
                     /locus_tag="ACG80L_001307"
     CDS             complement(16973..17935)
                     /gene="opp4B"
                     /locus_tag="ACG80L_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5023549.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17935..18879)
                     /locus_tag="ACG80L_001308"
     CDS             complement(17935..18879)
                     /locus_tag="ACG80L_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023550.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18880..19884)
                     /locus_tag="ACG80L_001309"
     CDS             complement(18880..19884)
                     /locus_tag="ACG80L_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023551.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5558 a         4280 c         3550 g         6580 t
ORIGIN      
        1 cggtagagta aacttatcaa ataaaagact tctttggagg aatcactatg aaggaatata
       61 taaaaaacat ctattttatc gaagaaacac aaaatatcga gggttcttat attgaagtaa
      121 aaacactctt tgtcaatgaa gataaaacca aagcattgga tatttataaa aaattagctt
      181 caaaaaaaac gaatagtttt ggattgatat taagcgagta taaaatcaaa gcagaagaga
      241 gttatttcta tcaattatta aaacggtgga gtaagcttcc cgccgatttt tatcgaaaaa
      301 tgcagatcat caactatcaa ccgcttgctg agacacatgc ctaaatagag gttgtgaaat
      361 cagtgctttg agctaatacc gaaggtcagc tgattgaaca ttgtttagaa gagattgtga
      421 caactgcgat ttgctttgag taataagaac gctggacaga aaatagcttc tcatattttc
      481 tagtctagtg ggcttattgc cgagaagcag cagatggaac atcgtttaga agagattgtg
      541 acaactgcga tttgctttga gtaataagaa cgctggacag aaaatagctt ctcatatttt
      601 ctagtctagt ggacttattg ccgagaagca gcagatggaa catcgtttaa agaagagatc
      661 ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg tacctttgat
      721 tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt tttgccactc
      781 cccattctag aatcaacccg tatgatgtag ataatgaatt cacgttgaaa aaagatttct
      841 caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg tttttgataa
      901 cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg ttacatagta
      961 tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat cttcgatcat
     1021 cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt caagggagat
     1081 gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag aaatatcatc
     1141 tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga tatccggatt
     1201 tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt cgatttgatc
     1261 atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac cagatccgta
     1321 ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag tagcttctgc
     1381 tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt tagcttcttc
     1441 ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt cccatggatt
     1501 tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt tttgaaaatc
     1561 tctttgtcgg ccagaaatga tgattagatc tggctgaagc tgattaattt tttctaagtc
     1621 tggttctttt attccacctg ctgattctac ttttttatag gatgaaagat actcaggaag
     1681 gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca tcgtatctaa
     1741 agaaccattg tcgaatacga cgactttttc tggattattt ggcacctcga tttggtctcc
     1801 attactatct gttatcgtca atgtagtttc agtttgtgtg ctgctttttt ttgtgtctgc
     1861 ctgactggtg ttgttgctat ttccacatgc tgctaaacca aatgatgcta gaatcaagat
     1921 acctactact aattttttca tctcttctct cctaatgtta gttttagaaa taaagacaga
     1981 atctgcggcc ttcaatctca caaatccgta tgtccatttc gtataattca tccaatacat
     2041 ctttttttat catttcttgg gtcgttcctt gcgtaaataa ctgtccatct ttcattgcaa
     2101 caatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata acaattgttt
     2161 tcccgatttc ttgtactaaa ttttttaaaa tctgcatgat ttgaaccgca aaattcatat
     2221 ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct agcaccattg
     2281 caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga tccgctattt
     2341 cttctaaccc aaggtgttcc agtgattcct gaacgatttc ctcgtctttt gtcgtcaatt
     2401 ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg atttggacat
     2461 ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg gatttccaag
     2521 cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc aaacgactga
     2581 tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca gtcaatttct
     2641 tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac agtttattga
     2701 ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg tgacaaaata
     2761 aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga aaatctgctc
     2821 tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc cgataaatag
     2881 ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa taaaccctaa
     2941 aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga cacaacataa
     3001 aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt catttccgag
     3061 atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc cgataatgat
     3121 caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa ataatctgcc
     3181 ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac tgacactacc
     3241 aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg ctctcatttt
     3301 tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa cgttcaataa
     3361 gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga agaaaaggac
     3421 cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca aaaactggtt
     3481 atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga caaggataaa
     3541 tgcaaaaatt ttcttactcc tcaatgcaca tgcaaaatgc cagtttccat atgtatggat
     3601 cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa aaatagatgg
     3661 ctttttttta agcatatcgt cgtcccctta tcagaagata aaggaagata gccccaccga
     3721 tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg cgagcgacaa
     3781 gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt gttttgtgca
     3841 cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata aaaggaatgc
     3901 ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg cctaaaaact
     3961 gcatcttctg gtaatctatt cccagattta ctgcaaacga ttcgcccaga ccaatgatcg
     4021 taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca tacaataact
     4081 cataatttcc tcgtatcaca gtcgcaaaat tcccttgaag ccatgaagtc atattttgta
     4141 ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc cctaacatga
     4201 ctccaatcaa cggaagcatc aattgatttt tctggaggaa caatcgcgta agggataaaa
     4261 aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca atcaaggatg
     4321 caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc atcgtgcccg
     4381 cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac ccacaaatac
     4441 tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc actagcaaga
     4501 tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc gatactccga
     4561 tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg tagcgcatcc
     4621 aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc gttgaacaaa
     4681 agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat cgtaagaaag
     4741 acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag ccatttggat
     4801 aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata tttagggaca
     4861 ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc aaaaaacaaa
     4921 gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt gaatggggct
     4981 ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa tagccgtttc
     5041 actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct cttaacagca
     5101 aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga agaaaactct
     5161 tcgccagctg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg tttattttaa
     5221 aacgaaaaaa cacataaatt tttacatgca aaaaaagctt ttgatcagat tgacatcaaa
     5281 taggacaatc tgtcaaaagc tttttctttt aaaatacttc aggatataac aatttcaata
     5341 cattcatcgt caatcttctg ccttttcctg caaaaggata aacatgcata tgcatggctg
     5401 gattgaaatt tgcttcgatg atcccatatg cttttttatc agttgtagga tcgatttctt
     5461 tatctggtat aatcaaatcg ataccactaa tcttagctcc tagtgcttct accgcagaaa
     5521 cggcgatttt tttgtaagct tcagaaaatt catcagtcat atcgatcgaa tcaccacctg
     5581 tactgatatt tgagttctcc cttagataaa caatttgatt ggcttgggga actgattcga
     5641 tcgtcaaccc ttgttctttc aacatcaatt gttctaattc acctaattgg atcaattcta
     5701 aaggtgcacg atgattcgtt ccacgcaaag ggtcactatt tttttcttca acaagttctt
     5761 tcactgttcg gatactgtca ccaataacat tagcagggac tcgcaacatg atggcttgga
     5821 cttctccgtc gatcacaaag aaacgatatt ccgttcccgg cataaattct tctactaaaa
     5881 ccgaactgtc ttcacgaaaa gcaatggcta aacctgcttg ataatcttcc agactagctc
     5941 cttccttgaa aatcgtgata cctaacccgt aattggttga tttaggtttg atcacaaatg
     6001 cctgctcggc aaatctcgga taagctttta ccgcttcttc catggatgaa aattctgcgc
     6061 ctcctggtac ccggaaccca gcttctttta agactttttt cgttactgtt ttgttttcca
     6121 tgataagtgg gacgatatag ctgtccttac ttgtcatgtt tgcatttttc acatattcta
     6181 catggtcttg gaattgcaga cgcaaaaatt gatccgactc atctaatact ttgacttgga
     6241 cacctttttg gatggcatcg aacataaata tctgagtaga aagttccatt tcgcgaaaac
     6301 cggccaactg atagggacgt tcatggcttt ctttataata ttgatgtccc aatgccacag
     6361 caacttcttg ctgactcgat agctgtgctt cttgatagag tttcgctgct aacgtttgtt
     6421 caggatgatc catttgagtc aatgcttcct ctactaaata aagagaatcc aaccaatcca
     6481 attcaaccag catttgcttc attccttcaa gaatttctct accttcttga tgaaattctg
     6541 tttgatctga tggttgttct agtgctactt tgttatttcg agtcgtacct tctgctaccc
     6601 attggtcaca gtcttctttt tcttctgtcc ataacatgta catcaggaat aattgtaaga
     6661 accgaacttg ctctggattg atacccaatc ttgcataagg attcaaatcg atattccgca
     6721 attcaatata acgaacacct gttgttgcta aatctgcgac tttctttccg cctcggaaac
     6781 gaagcggtgt ataaaattct ttctcctccg aaagtttccc ttcttcgatc attcgctcga
     6841 tatctgctaa atactgtttc atagaagcat aggaaacttt cacatttgga tgattcgtat
     6901 agcccagcgc actgttgcga atactgcgaa caggttcttg cggatgtgat tcatctaaaa
     6961 agtagttctt ctcactcatt ggtgaagcac cgaataaata agtgatcatc cagcgatagc
     7021 gcataaagtt tctcgctgtt ttcaaataaa gttctgtttt gaattcggaa aaatcttgaa
     7081 attcttcctg atggctgaac aatgttctta gcaactcatc gccaaattca aaattgaaat
     7141 gaataccgct taccatctgt tttcgcttcc cgtattcttt tgctaaataa cgtcgataca
     7201 agacatcttc gaaatttttt aattttgcaa taatgatctc ttcgtctttt tccggtaagg
     7261 caggtggcat acttaatggc cagatcatct cttctttcgg tatagaacga gcagtcacat
     7321 cataaacagc agcgagatac tgaaataatt cgggaataga atcggtaaca ggggtgatca
     7381 tctctgtttg tgtttcgcta aaatctgttt ggatataggg gtgataggat cgatcgccaa
     7441 agattgctgg atgatcggtt tttgctagat ttcctgcaag atcaacccgc tgtccttccc
     7501 gttctatacc aaatcgagct tggtcgataa aagggcgcgc attgacatgc aataataatt
     7561 gcttaaaatt catcattaag tgacctccaa ttcatatgaa tctaattata atcaaaaagt
     7621 ctaagaaaaa gaacgaaatt gtttgagaac cgcttacaaa taagtataaa aatgaaggca
     7681 tcaaaaacac agctatgtta aaaacaatca ttcaaacaag gagctgcaac ttttgtcaca
     7741 gctcctgctt tttagacatg ggttattctt cttttagtaa tcctttgaac aatccaacga
     7801 caaaatcatt tggatcaaac ggctccaagt catcgattcc ttcaccaaga ccgaccaatt
     7861 tcactggtaa atgcaattca ttacggatag caagtacgat accgcccttt gctgtaccat
     7921 ctaattttgt caaaacaaga ccggtcacat ctgttgtttc tttgaattgt tttgcttgaa
     7981 ccatcgcgtt ttgaccagtg gttgcatcta atacaagaag tgtttcttgg ggtgcttctg
     8041 gtaattcccg ctggattact cgttttattt tttctaattc gttcatcaag ttcactttat
     8101 tttgcagtcg gcctgcagta tcaactagta aaacatctgc ctgctctgtc tttgctcttt
     8161 ccaaagcatc gaatacgaca gctgctggat cgcctcctgc attgccacga acaacttcaa
     8221 caccagcgcg ttcgccccag acaactaact ggtcgattgc tccagcgcgg aaggtatctg
     8281 ctgctgccat caatactttt ttaccttcgt ttttgaactg atgggcaagt ttaccaatac
     8341 ttgttgtttt ccctacacca ttaacaccga caaacaaaat aactgttagt ccgtcttctt
     8401 ggatatttag ttcatttact tcattgattc cttccgcttc atagagatcc acaagtttct
     8461 cgatgatcgc attttggact tctgccgatt ttttcgcatt tctgagtttc acttcttgac
     8521 gtaatgcttc ggtaattcgt atcgctgttt caaaacctac atctgcaccg ataagtgttt
     8581 cttctaattc ttcaaaaaag tcttcatcga ctgttcgaaa attggcaaac aactcattca
     8641 tccgttcacc aaatgtttta cgcgtctttt ctaaaccctt gtcgtacttc tcctgtactt
     8701 caacttcttc tgttttctct tcgccagtaa aagcacgctt gattttatca aaaaatccca
     8761 tttgactccc cctactctaa tacaaatttt tggatcactt tggctacgcc attttcttca
     8821 ttgctggctg tcacgaaatc tgctgcttct ttgacgaaag gcacagcatt ctccatcgct
     8881 acgcctagtc cagcatattg gatcatcggt aaatcatttt cctcatcacc gatcgccatg
     8941 atttcttctt gtttcaaacc aagatctttt gccaataaag aaataccata agcttttgta
     9001 acaccttttg gcataaattc caaaagcata cttcttgttt tgatgatctc atacatttca
     9061 tggaattctg ctggtatttc tttgatttta ggatctaatt cttcttggtc ataagcgatt
     9121 actgccttat taagcacaaa ttcatctgta atatcgctta tatccgctgg ttggaattgc
     9181 agcagcttat tcaacacatt gtacaaggat tgcttccttg gggcactagg cagctgtaaa
     9241 accacattgt cagacaaaac atctagtggc agatccaatt gctgagccag tttgtataag
     9301 cgttggatat ctgatacttc catcactttc ttttccatga tttcaccagt atcatttttt
     9361 tgtaccaaac cgccgttgaa agtgatgctg tagtcacctt catctaccag tcctaattct
     9421 tgcagataat gcgccatcgc agctaagggg cgtcctgtac atagaacgat cttgacccct
     9481 tgctgttttg cttgtgctaa tgcttgttta ttctcatctg aaatcagctt ttgttcattt
     9541 aataatgtac cgtccaaatc gattgctact aatttgatca ttttcgttcc tacactttcg
     9601 tttcgttgat tttcgcctct tcattgatat cttccatccg tacagaaacg atttttgata
     9661 caccggattc ctgcatcgtc actccgtaca gtacatctgc tgcgaccatc gttcctttgc
     9721 ggtgtgtgac tacgataaat tgtgtgtcat cttggaaatc gctcaagtaa cgaccaaaac
     9781 gtttcacgtt ggcttcatct aacgctgctt ctacctcatc taagatacaa aacggaacag
     9841 gacagacacg gataatggaa aagagcaacg cgatggctgt aagtgctcgt tctcctccag
     9901 aaagcaaact tagactttgc agttttttgc caggaggttg tacttcaatc tcaatacctg
     9961 ttttcagcaa atcagttggt tccgtcagta ctaattcagc tttaccgccg ccaaacatat
    10021 ttggaaagac aattttgaat tcttgacgga tagcttcaaa tacttctttg aatcttgtcc
    10081 gcacttcatc atccatctca tccatcgttt caaacaattg gtttttcgct gctaacagat
    10141 catctcgctg tgttgctaag aatgtatgtc tttcactgac ttgttcgtat tgttcgattg
    10201 aatttaagtt tactggtccc agtttttcaa tctgttcttt taaggatgaa acctttgttc
    10261 tactgtcttc gatatcggtt gtttcttgat agtcttggga agctttctca aatgtcagct
    10321 gatattctga ctgcagatag gtcaaatgat tatctaaaat catttcagct cggtcttttt
    10381 ggacttctaa tttggattgt tccgacagtc gtgctttttg ctcacgatta cgctctgcca
    10441 agactgcttc aagcttatcg atctcctttt gccttctatc tcgctgctct ttggcttttg
    10501 ccaactcggc ttttaatgtt tctcgctgag cagataattc gttaatttgt ttttccaaac
    10561 tttcttctgt gacttcatga tcagaaaagt ctgctgtcaa tgtcgccaac tgcttttcta
    10621 tcgcttcttg acgttcggtt gcctctgctt tttggactcg ggcaccgcgg agctggatct
    10681 gcagatgatt aaactgctct ttcaagacag caagatctgc ttgttcttgt gctttttgac
    10741 tttgtacttg tgctcttctg gcttccatct ggtcgctttc ttgacttaac gatttgattt
    10801 cttcgtcgat tttttgtcgt tgcgattcaa gatctgtttg tttgtctttc aattcgttcg
    10861 tttgcttttc gtaatcttcg ataaactgct gcagttcacg tgtctcaaaa gttgagattt
    10921 gcttttcttt ttcaaaacgt tcaagttcat ttatgatgtt ttgtagctga tttgtcgctt
    10981 cttgttcttc aaaacgtaat tgttcgcctc gtgtacgcaa aacttcttgt tcttcggcta
    11041 aacgagctgt ttcttgttgc agctcctgaa cttttttttc ttgtgcttgg agttgtttat
    11101 ctgcttcttc aaattcacta gtcaactgtt tcaattcttg attttgtaca aacaaactgc
    11161 cttggtttcc tcgtttattc gctccacctg tcatggatcc tccggcattc atcacatcac
    11221 cttctaagga aaccactcga tattgatagc ggatcgtttg cgcgattgca ttggcactag
    11281 ttaagtcttt ggctaaaagg atcgttccta gcaggttttg aaccacagtt tggatttgat
    11341 cgggataact gacttgttca ctggcaatcc ctataaaccc ttctacagct gctgcttgtg
    11401 tcaatatatg agccggtaat tgtcttggtt tgattgtcgt cagtggaagg aatgttgctc
    11461 ttcctcctct ttgctgtttc aaatacgtga tggcttgccg agcatctttt tcattttcaa
    11521 cgatgacatg ctgggctgcc ccacctaaag ctgtttcgat agctaacgtg aaatctgcag
    11581 gaacatcgat cagttcagcc actgctccga caatacccga aagctgctgt ttatgctgca
    11641 agaccaatcg aaccccttgg taaaaaccaa aatagttttc ttggatttct tgaaggcttt
    11701 tcttacgagc acgtagctgc tggacttgat tcattaattg atacatttta ggctgttctt
    11761 ttgctaatgc ttcttggaac tttttcccgt tttgttggat catttctaac ttctcttttg
    11821 tttcagttaa agatgatttt agctgcgcct gcttttgtgt caattcttct ttttgtaaga
    11881 tcaatcggtc tactgatgct tctacttccg attgcttggc taacgtctgc ttgctttttg
    11941 ctgtttcttg tatatattga cgttctagat acttcagttc gtttcctaca gctgcttgtt
    12001 cttgcatcaa atcaacatat tgatctctta attctgccaa taattctttc gttgattttt
    12061 ggtatttttc taattcttgc tgtgttttta tgagatttgc ttcagctttt tggatctctg
    12121 tttctttttc tgctgctgct ttcatcaagc tttcttgaag cttttcaaaa tgtttaacct
    12181 ttttttgtgc ttctgctaat gaagtttgat attcttgcga gcttttttgt gtatgctttg
    12241 tccgctcctg caaaacatct ttttgtcctt ctgtctgctt cagtttttcc gaaagatcta
    12301 ataatacttg ctgatttttt tcgatcagac gatcggcttg cgcattttct ttacgctgct
    12361 tcgccaaaat ggattcttgt tcttggatcg actcagatag tttgcctaat tctagattga
    12421 atttcgcaag ctgtgcttgt ttgttgtccc aatctttttt tgctgtcttg atttcagcaa
    12481 ccatcaaaga gacatcggtt tgagtcagtg tttcttttag ccgtaaaaac tcctttgctg
    12541 cctcactctg ggcggccaaa ggagttaact gttcttccaa ttcgtggatg atgtcttgga
    12601 cgcggctgag gttgtcttct gtttcaaaaa gcttctgttc tgctttcttt ttccgctgct
    12661 tgtatttcaa gacaccagct gcttcttcaa agatcccacg acggtcttcc ggtttactac
    12721 tgaaaatcgc ttctactttt ccttgagaaa tgattgagaa cgattctttt cctaatccag
    12781 aatctaagaa caattcttgg atatctttta atctgcagct ttgtttattg atgaaaaact
    12841 cgctttcccc agtccgtcga taacggcggg ttacactgat ctcattgtac tccaatggca
    12901 gataatgatc actattgtct aaaatcactg tgacttctgc aatattcagc tgcttacggg
    12961 tatcggaacc ggcaaagatg atatccggca tttttccgcc tcgcagactt ttagcagatt
    13021 gttctcctaa tacccagcgg atagcctctg taatattgct ttttccgctg ccatttggtc
    13081 caaccactgc tgtgacgcta ttttcaaaat caatgactgt cttgtctgca aatgatttaa
    13141 aaccggctat ttcaattcgt ttcaaataca aagctttccc tcacttactt ttacagtgct
    13201 gccaatgcat tttccgccgc agcttgttct gctaattttt tcgatttccc ttgtccagtg
    13261 ccaatcaatt gatcatctac ataaacttcg atccaaaata ctcgttcatg agctggacct
    13321 tcttcattga tcaatcggta ttcgattgat acatcacctg atttttgcaa gacttcttgt
    13381 agttttgttt tatgatccat ctcatgtgaa aaagcacctg ctttgacttt aggaaaaatc
    13441 actttttcga tgaatgcatg ggtcgtgtcg aatccctggt caagatataa cgcgcctaaa
    13501 aaagcttcga ataggtcgca aagcaatgct ggtcgcgtac gtcctcctga attctcttct
    13561 cccttgccca acaaaacata ttgatcaaag tgacattctt tggcaaattt cgacaagctg
    13621 tcttcgcgaa cgatcgcagc tcgtgttttt gtcaattttc cttctggcaa atgaggatat
    13681 aaacggaata aatactgaga aaccatcagt tctaaaacgg catctcctaa aaattcaagt
    13741 cgctcattat ccgataattg cagattgcga tgctcattca cataggatga atgggtaaaa
    13801 gcttgttcaa gcaaatttag atcgtgaaaa acgatgttat agttttcttt tagttctttt
    13861 gtcagctggt tgtccatttt ccacattcct tttatggata ttttttcgat acttccttat
    13921 tatactggtt ttttgtcaaa tttgaaatgg ttcggtcaaa aggtaagtaa agctttaata
    13981 atcctagtga ttgacgagaa gttgtgaaaa attcgtttag tttcgagtat gaagaaaaaa
    14041 ttttgtgaaa atggctttcc atatttttgc ataaatttga cttaataccg agaaactgga
    14101 ctttgaacac cgtttattgg agaagtcatg aaatcagcgc cttgacctga atattagaga
    14161 aataaacaga aaaatatcaa aaaaccgaaa aaggacatcg ccctttttcg gtcattttta
    14221 gtgattatga acgtgaatct gctgtaacag aaacatttgc ccatgggttt ggtacatcat
    14281 aagaccaatc ccagtctttg acacggtcgt tgactggcaa tacttcatta cggaacaacg
    14341 ttggaatcgc aaatgcttct tcaaatgcat attgttgcca tttttgatat gcttcttttt
    14401 ggtactcttc atcaaatgct ttgcttgaac tgatatcgtt gatcaatttt gtattttctt
    14461 ctgattcata acgagtgtag ttgaacgcag catttggtcc ccatagtcca cttggatttg
    14521 gatcagttcc tgtattccaa gcagcttggt aaacatcaat ttcaggatca tcgttttcta
    14581 atttatcata gaaagaattg aattcgatca gacgacctgt tgtgtattgg acatccaaac
    14641 caatttcttt ccattgttgg atatagtaat ctgccaatgg ttgcgctgtt tccccaccgg
    14701 acatcgaagc aaagttgatt gttagtttat ttccatcttt gtcttctctt aaaccatcgt
    14761 catccacatc tttgtagcca gcatcatcta atagtttttt cgctttgtct agatcttgtg
    14821 tgaatccttt catctcatca tcatggtatg actggaatac tggaggaatc aatgaagtcg
    14881 cattgcttct caaacctgca tagaaacgtt cacctactgc attattatct aatgcatagc
    14941 ccattgcttg acgtaatgat ttatcagcca tttttgaatc tggattgtat tccacactct
    15001 ttgtatctgc attccattta cctaacttga atcctagata agtataagaa gtttgttcac
    15061 ggcctaagtt tgtatagcca gcaatttctt tataggtatc gtaagtatct gttggcattt
    15121 ggaaaatcat atcatataat ttagagttca atgcttcagt tgcacttgcc atcggtacag
    15181 atttcatcgt gatctttttc aatttaggcg taccattcca gtagtgctcg tttggcaagt
    15241 attctactga ttctcctgca acgatattac tcatgtaata agggccaaaa gtcactggat
    15301 ttttacgtac ttgatcgctt gattccatat ctttgattgc tacaccttct aacgtatgtt
    15361 ttggtgctgc atatgaccag ataccaccac ccgattgaag catgcttggg tttacttctt
    15421 tatattcgat ttcaactgtt ttgtcgtcta tttttttgat tccggagatt gtgtcggcac
    15481 ttccatcatg atattcttcc atacctacga tgttacggaa agtgtcgtca taacggatac
    15541 cagtgtaatc tttattccca atgatttcgt aagggaaaat cacatcgtcc gccgttacat
    15601 cttcaccatc tgaccatttg acgttgtctt ttactttgat tgtcgctttt ttcgtatctt
    15661 catctagatc aagcgttgca gctccgtcgt ctgtgattct gaaatcgtca tcgtttgcaa
    15721 atagcggttc atgagaagga gccataaaag ctgcatcata tgaatcttgg taaaactccc
    15781 actggaacaa tcctttaaac tgactgtccg ttgcgaccgc agcttctaat tcgccgtctt
    15841 cgattgcatc atcattattt tttgttgcaa ttggaaaagt taattcctct ttgttattgc
    15901 cgttgcctcc agagttgttt ccagaggtat ctgtacctcc gcccccgcat gctgcaagaa
    15961 cgacagcaga catcagcgct gcaaatccaa aaatcgtttt cttcattcct atttccccct
    16021 tcgcttctat actagcctaa ccgttggcgc gcatctgcag aacgcttgaa tgcttgtcca
    16081 acataattga tactcaacat caaaactaaa ataaggattg atgcgggtaa ccagatccat
    16141 tgccgattca ctaagacttc tccgttactt gcataaccga tcaatgtacc taaactaggt
    16201 acatttgccg gcaagccgaa acctaagaaa gtcaaagttg tctcgatacc gatgtttgcc
    16261 gcaaagttca tcgtcaaatt agtaatgatt aatgaactta gattcggcat gatttcgcgg
    16321 aacatgattt taaaatcatt ggttcccatt gtttttgagg cactgacata atctcttctt
    16381 gcttcagata atgtcttgct tcggaataat cgggctttag ctacccaata aaatgcactc
    16441 atgatgaata taaaggacca gacattgtat ctggaaataa tcgatacgaa gacgataatg
    16501 atcagcatga tcggtagaat catgataaaa tcaacgatcc gcatcaagat gttgtcaatg
    16561 atccctccgt agtagccaga gatgatccct aatccaaccc cgatgatcga agtaaggatc
    16621 gtgatcgcaa atccgatcgt aacagagttt ctagcaccta tgatcaattg tcctaataca
    16681 tcacgtccgc cttcatctgc tcccagaatg aacttttgtc cttgtgtcga aactgttcct
    16741 ggtgcagcgt atttgtcgaa gatactaaca tacatcactt tgtcttgatc ggtcagcatc
    16801 gcaccgataa agatcactaa taataaaacc actagcaaac ctaatgaaaa aatagcaatt
    16861 ttatcttttt taaattctcg agcaatcatt cgtatcccca taggaggaat actttcttga
    16921 actgctgtat tgtttttttc actcatcgat ccattctcct ttctgattgg ttttattgaa
    16981 tccgaatacg cgggtcaacg atactcatga tgatatctga aagtaatgtc cccactagcg
    17041 tcgcgattcc taaaatcaat actaaggatg tgatcacggt atagtcccgt tggccgatgg
    17101 aatcaataaa tagttttccg actcccggat aaccaaaaat cttttcgata actacagaac
    17161 cagcaatcaa cgaagtgatt tcatacccca tttgagaagc catcggcaac gctgcatttc
    17221 ggaaaatatg tcgactgaat actttattcg tcggtacccc tttggaacgg gctgttctga
    17281 caaaatcgag cgatttcgaa tcgattactt cacttcgcag atattggatt gtgacggcag
    17341 tcgccaaaaa tgcttgggta attgatggta acagaatata tttcaaacgg tctatccatt
    17401 gagtgataaa tccaccttgc aaacctagtg tttgtgatcc acttgttggg aaccagccta
    17461 aacgataacc aaaaacaaat agcatgatca agccgaagat aaataaagga acagcaaaac
    17521 taaagaagtt gtagattacg acaatcttat ccaagattga attttgatac cggccagcaa
    17581 gcatccccag aggcacagca atcaaataag taaggacaac tgtcaagatc gatagatata
    17641 aagtgttgat gatccgttcg ccgatcaagt cagcaacaga gcgtttgtat aagaaacttt
    17701 gaccaaaatc gccttgcaaa gcatttccga tccaacggaa gtactggaca taccatgggt
    17761 cattcaagcc agctgcttgt cgcatctgtt cgatcactgc cggatcctga tttggattga
    17821 tcaaaccggt aaatggatcc cccggcatcg cttgggctaa taagaagatc agaacactta
    17881 aaataataac ttgcgggatc atcaataaaa cacgtcgcag aatcgttttc cacattagct
    17941 tgcacctcca tctttcaacg ccactttgtg tgtagttgtg atcgtacgca aatcgtagac
    18001 tcgaccagtc gcatcatagt attctttttg gttttggata tactcttgtt ctactcttcg
    18061 tcgattttct ttgtggcttt ccctattttc tacatcgatt tgcggaatag ctgaaagcag
    18121 acgtttcgta tagatatgtc gcgggtcatt ataaatgtct tctcttgtac cgatttctac
    18181 aaatcgacct ttgtacataa ttgcgatatt gtcgcacatg tgttttacta cacctaaatc
    18241 atgtgaaatg aataaatagc tgaggccgta ttcttgctgg attcgtttca tgaaattcaa
    18301 tacttgcgct tgaacagata gatccagagc agatacaggt tcatcggcta cgatcagttt
    18361 tggattggta gcaacagcac gggctactcc tagacgttga cgttgtccgc ctgaaaattc
    18421 atgaggatat ttatatagcg catcttccgg catcccaacg atatctagca gtccttttac
    18481 ctttttcttt tcttcttgat cgcttaaccg ttcaaaattt cggatgggtt cagcaatgat
    18541 atccaacact cgctttttag gattcaaact ggacattgaa tcttggaaga tcatctgcac
    18601 atccttgtta taacccattt ttttgcggtt gcttcgcttc gttacatctt ttccttcata
    18661 catgatttgt ccggctgtta ccttttctaa tcccacaacg gcttttcccg tagtagattt
    18721 tccagagcca gactctccca ctaaaccata tgtctttcct ttttcgataa taaaatccac
    18781 accatctaca gcatatacat gatccgttac tcgattgaag aatccactgc gaatagggta
    18841 atgaactttc aggtctttga ttgtgattaa ttctgtcatc tacacctctc ctttttcgtc
    18901 tctaaagtgg aaatgcttat agcaggagca acgtaccaag tggttcggtg cgacttcatg
    18961 aaggataggc tcttcttcat gatcgcttgc tggtatccat ggaatacgtg gtgcaaaccg
    19021 acaaccttta cgaggcatgt ttttcagaga aggaacgaca ccttcgatca catggagttc
    19081 tgcatcatga tttccttctt gtggtataga gttcagtaaa gagcgtgtat aaggatgctt
    19141 aggatgatca aacagttctt ttacacctgc tacttcaacg aactgtccgc catacatcac
    19201 tgccactcga tcagccattt ctgctacgac tcctaagtca tgagtgatca agatgatccc
    19261 tgtttttgtt tcttcctgaa ggtctttcag taaatcaaga atctgtgctt ggatggttac
    19321 atctagggct gtcgtcggtt catctgcaat gacgattgga ggtttgcagg cgattgcgat
    19381 tgcgatgatg acacgttgtc tcataccgcc tgatagttca tggggatatt ggcgtccaac
    19441 tctcgcagga ttaggaatcc ctacttgttc tagcaattct agtgcacgag cttgacgctg
    19501 ttctgcattc atctttgtgt gataggaaag tccttctttg atctggtctt cgattctcat
    19561 gagaggattt aatgcagaca acggatcttg gaaaatcatt ccgatatcat ttccgcggat
    19621 tttattatag agcgtttcgt tcaggtcgat taaattcaga tcgttatata aaatctcacc
    19681 tgtaattttt gtgttattag gatcaagtaa tcccataatc gtagtagcca aggtactttt
    19741 cccgcaccca gattccccaa cgattgctaa aatctcgttt ttgtctaaag taaatgaaac
    19801 gtcatctact gcatcgtaat agtcatcttt gagtcggaat cctgtatgta ggttctgaac
    19861 ttctaataat tggttatcgg acaatcgaat acccctctct gcttcctaaa tggaagtttt
    19921 tcttaataaa aaaggacatt acacagaata aatcgtttta taacaaag
//
LOCUS       DAZUMB010000041        19480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000041 DAZUMB010000000
VERSION     DAZUMB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.041"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG80L_001310"
     CDS             133..3660
                     /locus_tag="ACG80L_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS5023552.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG80L_001311"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG80L_001311"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS5023553.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG80L_001312"
     CDS             7448..7789
                     /locus_tag="ACG80L_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5023554.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG80L_001313"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG80L_001313"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS5023555.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG80L_001314"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG80L_001314"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS5023556.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG80L_001315"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG80L_001315"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS5023557.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG80L_001316"
     CDS             13000..13737
                     /locus_tag="ACG80L_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5023558.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG80L_001317"
     CDS             13751..14566
                     /locus_tag="ACG80L_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5023559.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG80L_001318"
     CDS             14579..15226
                     /locus_tag="ACG80L_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5023560.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG80L_001319"
     CDS             15242..15898
                     /locus_tag="ACG80L_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5023561.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG80L_001320"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG80L_001320"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS5023562.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG80L_001321"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG80L_001321"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS5023563.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG80L_001322"
     CDS             18240..18758
                     /locus_tag="ACG80L_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5023564.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG80L_001323"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG80L_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS5023565.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6552 a         3344 c         4182 g         5402 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag
//
LOCUS       DAZUMB010000042        19257 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000042 DAZUMB010000000
VERSION     DAZUMB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.042"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..2631
                     /locus_tag="ACG80L_001324"
     CDS             160..2631
                     /locus_tag="ACG80L_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5023566.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2734..4470)
                     /locus_tag="ACG80L_001325"
     CDS             complement(2734..4470)
                     /locus_tag="ACG80L_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5023567.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4467..5171)
                     /locus_tag="ACG80L_001326"
     CDS             complement(4467..5171)
                     /locus_tag="ACG80L_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5023568.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5302..5804
                     /locus_tag="ACG80L_001327"
                     /pseudo
     CDS             5302..5804
                     /locus_tag="ACG80L_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
     gene            5764..6102
                     /locus_tag="ACG80L_001328"
     CDS             5764..6102
                     /locus_tag="ACG80L_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS5023569.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6123..7541
                     /gene="ffh"
                     /locus_tag="ACG80L_001329"
     CDS             6123..7541
                     /gene="ffh"
                     /locus_tag="ACG80L_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS5023570.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7653..8231
                     /locus_tag="ACG80L_001330"
     CDS             7653..8231
                     /locus_tag="ACG80L_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS5023571.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8235..8756
                     /locus_tag="ACG80L_001331"
     CDS             8235..8756
                     /locus_tag="ACG80L_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS5023572.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8835..9389)
                     /locus_tag="ACG80L_001332"
     CDS             complement(8835..9389)
                     /locus_tag="ACG80L_001332"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS5023573.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9642..9917
                     /gene="rpsP"
                     /locus_tag="ACG80L_001333"
     CDS             9642..9917
                     /gene="rpsP"
                     /locus_tag="ACG80L_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS5023574.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9929..10180
                     /locus_tag="ACG80L_001334"
     CDS             9929..10180
                     /locus_tag="ACG80L_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS5023575.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10305..11096
                     /locus_tag="ACG80L_001335"
     CDS             10305..11096
                     /locus_tag="ACG80L_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023576.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11266..11787
                     /gene="rimM"
                     /locus_tag="ACG80L_001336"
     CDS             11266..11787
                     /gene="rimM"
                     /locus_tag="ACG80L_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS5023577.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11777..12538
                     /gene="trmD"
                     /locus_tag="ACG80L_001337"
     CDS             11777..12538
                     /gene="trmD"
                     /locus_tag="ACG80L_001337"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS5023578.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12674..13021
                     /gene="rplS"
                     /locus_tag="ACG80L_001338"
     CDS             12674..13021
                     /gene="rplS"
                     /locus_tag="ACG80L_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS5023579.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13153..14289)
                     /locus_tag="ACG80L_001339"
     CDS             complement(13153..14289)
                     /locus_tag="ACG80L_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5023580.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14405..15319)
                     /locus_tag="ACG80L_001340"
     CDS             complement(14405..15319)
                     /locus_tag="ACG80L_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS5023581.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15410..15838)
                     /locus_tag="ACG80L_001341"
     CDS             complement(15410..15838)
                     /locus_tag="ACG80L_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5023582.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15843..16217)
                     /locus_tag="ACG80L_001342"
     CDS             complement(15843..16217)
                     /locus_tag="ACG80L_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5023583.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16503..16688
                     /locus_tag="ACG80L_001343"
     CDS             16503..16688
                     /locus_tag="ACG80L_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5023584.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16700..16948
                     /locus_tag="ACG80L_001344"
     CDS             16700..16948
                     /locus_tag="ACG80L_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023585.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            16979..17158
                     /locus_tag="ACG80L_001345"
     CDS             16979..17158
                     /locus_tag="ACG80L_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023586.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17201..17671)
                     /locus_tag="ACG80L_001346"
     CDS             complement(17201..17671)
                     /locus_tag="ACG80L_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023587.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17758..18456
                     /locus_tag="ACG80L_001347"
     CDS             17758..18456
                     /locus_tag="ACG80L_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS5023588.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18443..18577
                     /locus_tag="ACG80L_001348"
     CDS             18443..18577
                     /locus_tag="ACG80L_001348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023589.1"
                     /translation="MTMNRAEALRIGTAIANCWWKYYKPIILSQQHIDKQKAWQQIKK
                     "
     gene            18634..18975
                     /locus_tag="ACG80L_001349"
     CDS             18634..18975
                     /locus_tag="ACG80L_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023590.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            18968..>19257
                     /locus_tag="ACG80L_001350"
     CDS             18968..>19257
                     /locus_tag="ACG80L_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5023591.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6342 a         3368 c         3790 g         5757 t
ORIGIN      
        1 ccaccaacac aaaatattat agaacccttt ttttttgcta tatgacagga atgaaaggtg
       61 tttgcaagct taatgtgagg aacactttcc atatctgttc tatactggat aggagaagtt
      121 tggaaatatc cagaaaacga aatcaatcga ggtagccaca tgaagaataa atggataaat
      181 cgaagtaaaa aaaagaaagt aacacaaaga tcatcagata aagtcatccg tctttttcca
      241 tcgtataaag aaggattaac ggcagatcaa gtcaaggaac gaatagaaaa aggtgcagca
      301 aataattcag tcgatcccac attcaaaaca aatcagcaaa tcgtattgga aaacatattt
      361 acgtatttca atttgatctt tcttatctta gccattttgc tttgtttagt agaatcctat
      421 aagaacttga cttttttacc agttatcata gccaatacag gaatcgccat ttttcaggaa
      481 attcgatcca aaaagatctt ggacgaacta aatgttttaa atacagcaaa agtcaatgtc
      541 atacgagacg ggatagaaca aacaatagat atagaagacc ttgtaataga tgacattgtt
      601 attttagaga ctggatatca aattccagca gatgcagagg tcgtggaagg gaaactgcaa
      661 gttaatgagg ctttattgac aggcgaagca gatgaaataa ccaaagaaat aggtgatcca
      721 ttaatgtccg gcagtttcat tgtttccgga aaagcttacg cccgattaga caaagttgga
      781 gcagattcgt atatttccca gctgacagtc aaagccaaga caatgggaga aggagaacag
      841 tcggagatga ttgcttctct taatcaattg atcaaatgga ttgggatcat cattattcct
      901 atcggtatcc tgcttttttc tcaaagttac ttttttaatg gaaacacgat caaagaaagt
      961 gtcgttgcaa tggaagcagc attgatcggt atgatacctg aaggactgta cttgctgacg
     1021 acgattgcat tagcattaag tgcgactaaa ctagcaaagc aaagagttct tttacacaat
     1081 atgaaaagca ttgagacatt ggcaagggta aatgtccttt gtgtagataa aacaggaacg
     1141 atcacagaaa ataaaatgtc tgttcaaaaa atgatcgtct ctaaaaaaca agaaataatc
     1201 agtcagtcag atcaattgga agcatgcatt gctgactatg caaaagcgat ggcaggagac
     1261 aatgcaacaa tggaagctgt taaagcatat tttacaaaaa ccactgatca atcctatcac
     1321 catgttattc ctttttcttc tgtccaaaaa ttcagcagtg tagcgttggc agacaaggtg
     1381 tatgttttag gagcgcccga aatggttttg cgcgatcaat tgccggaata tgcggaagag
     1441 tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct ttggtgtgta cgatgggatt
     1501 cttgaagaac cagtattgac agaagcagtc acaccattag gctatattct gattgctaat
     1561 cctatcagaa aagaagcaag agccacattt gattacttca aaaaacagca agtagccatc
     1621 aaagtgattt ctggagataa tccgctcacc gtatccaatg tagctgtcca agcagggatt
     1681 tcacatgctg atcagtacgt tgatgcatct aaattatcag aagaagaata tgaagcagct
     1741 gtcgaaaaat atacagtttt tggacgcgtc aagccagaac aaaagaaaat cttcgttcaa
     1801 ttgataaagc gtaacaatac agtagctatg acaggtgatg gcgtgaatga tattctagcc
     1861 atgaaagaag cagactgcag tatagcgatg gcttccggaa atgaagccgc catgcaggcc
     1921 tcacaagtgg tcttacttga ttctgacttt tcacgcatgc cagaagttgt tgcagaagga
     1981 cggcgcgttg tgaacaatat tgagcgatca gccagcttgt ttttagtgaa gaatattttc
     2041 tcctttttgc tctcactgtt ttcagtgatc tttgctctga cctatccttt agaaccatca
     2101 caaatcacat tgattagttt gtttactatc ggacttccat ctttcttgct tgctctagaa
     2161 gaaaacaaaa agcgaatcag agggaaattc atcatgaatg tgatggaaaa agctgtgcca
     2221 ggtggactaa cagatatgat cgtagtcggt gcgttggtga tctgcggggt gactttgaat
     2281 ctaaataaaa cagatgtttc tacagcatct accatgcttt tgattgcagt agggttcctc
     2341 gttctctata aaatctgttc accgctgaat aaattcagaa gtcagatcat tcttttctgt
     2401 gctagcggta actttttttc ggtcatcttt ttgcataagc tgttttcaat caccggtatc
     2461 tcagctgtat cacttttgct gttgagcatt ctatttttct cggcagattc gatttttagg
     2521 catttgacca cattggtaga atatttgttt gaacgtaaaa atgaaaaaga gcaaaagccg
     2581 tttagtcgcg tagctaaaaa atttctatcg atatttaaac gagaaaattg acgaaaagta
     2641 tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct ttcgaagcag gatgagtcat
     2701 ccaactttaa aacgcacgag cagttgctct tttttacatt ttcggaatgg taacggtgaa
     2761 agtcgaacct gttccaagat gactgtctac agtaatcgta ccattaagga gttgcacgta
     2821 gtcttttaca atcgctaacc ctaatccggt cccgcctgaa tgtcggcttc gcgccttgtc
     2881 tacgcggtaa aaacgctcaa agatccgttc ctgatcctca atgtcgatac ctataccaaa
     2941 gtcttggaca gaaaacgata gatcatctgt tgcctgatag cggataataa tctcgctgtc
     3001 gctttttgaa tattggacag cattttcaat tagatttttg ataattggat aaaggatatc
     3061 tgttttagta gtaaatggtt cgttctttcc aatcaatcga atcgtcaacc gtttcttttc
     3121 gatgattggc tgataatttt gaacgatttg ctggaaataa ggttctgtat aaattgtctg
     3181 gatctcgtaa gaattctcgc catcttttga cagctggatg atttcccgaa tcagtttatc
     3241 taaccggatc gcatcttttt gcatgatctc aagaaaagaa gtcagagttt gcgggtcatc
     3301 ttttgcacca tccagtaaag tctcggtaaa accaatcagt gaagtcacag gagtttttaa
     3361 ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt tctaaacgtc gcactttcgt
     3421 taagtcatac gccaccccta gaatctgtcc ggtaccttct gaattagtaa agtagcgcaa
     3481 actcaagtct aatgtctgcc gaccttctgt aagggtgatc tcctgatgga tcagcggatg
     3541 ctcaggagtc acttgatgaa tcagctgaat gagttttggc tcttgaatga cttctgtgta
     3601 ccgctgttca ggttgatagc ttaccacgtt taaatgtagc tgcatctttg gattaagcag
     3661 tagtaaacga gaatcagcat catcaatcag gaatacgcca atcatcagtt cgttcagtaa
     3721 cgtatataat tgctcttctg tggtagtaca cgcccgatac atcttactca tttgttcgct
     3781 caacgcatta atcgtctgat acaattcttc ccattgatcg ggtgtttgca tgattacttc
     3841 cgcctgatta ggattttgca ccattttttt tagtacgggc agcacggttt tcaatggctg
     3901 gtttttttga tagatcagat aatagatcat tcctgcaaga acaaggaaga aagacagaaa
     3961 gaagaaaaag acccaacggc gaaaactctc agttctcggt aaaaaaccgc ttgtcggttc
     4021 tgcgatacgt aaaatggctt ctaactggcc actttttttg actggtaatg cgacatataa
     4081 aagatcctca ttcaaagtag tacttttgcg caaagcgcta cctaggttcc caccattcaa
     4141 tacggctttg atttcgggac gggagtttcg ctgttcattc aatgtttcat tgtttgtgtc
     4201 aaataagatc tttccagtag catccattaa agtaatccgt tcgttcgact gatggacgaa
     4261 ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt tcgacatcta attgccggat
     4321 cagcagttgt gctttttttg tcaggtagct ttcttgctgt tccacgatct gctgttgaaa
     4381 ataatgggaa atcaattgcc agccaccaaa aaacaaacta actaaaagaa taagaaagaa
     4441 gagacgttca taaatttttt tccatttcat cgtttgggtt cctgaaactt atagccaaat
     4501 cctctaatag tcaccaagta ctttgggtgt tttggatctc gctcgatttt ttctcgcaaa
     4561 tgactaacgt gtacatctac gattcggctt tgtcctgcga agtcaaaatt ccaaatgcgg
     4621 tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt tcatgaaata aacaagtaat
     4681 tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta cggtcacttg ataattggtc
     4741 aaatcagcag tgatttgtcc aatcgataga tattcaggct cagcttcatc ttttgattgc
     4801 ttacgaggtt caattcgtct gaatattgct ttcatacggg ctaatacttc gcgaggacta
     4861 aatggtttgg tcaaatagtc gtcagcacct atttctaggc caatgatccg atccacttgg
     4921 tcatctttag ccgtcagcat caaaatcggt gtttcatttt tttcttgacg cagccgctga
     4981 gtgatctcca tgccatccat cgttggaagc atcacatcta aaatgatgaa atcaaatgat
     5041 tgttctaatg ctaattctaa tccttcgcct ccatctgtgg cactggttac cttgtagcct
     5101 tctttttcca aattgaacgt caagagcgtt acaatagaag gttcatcatc aacaactagt
     5161 atttttttca ttcaaatctc ctttaaatag gtttcattct gtttatattt tagcatagaa
     5221 tcagtacaaa gggctatttt acaaaagaaa taaatggtat aataggaagg cactttgctg
     5281 aatagaaagg aagagcagtc aatgattgga atcagtgcat gtttaggcgg tctggcctgt
     5341 cgctatgatg gacagaaaaa agaagtacca gaattgatgg aactagttga aactggagaa
     5401 gctgtcatgg tctgtccaga agtgatcggc ggattaccaa ttcctagaga tcccgctgaa
     5461 atcattggtg gaaatggatt cgatgtgtgg aaagatcaag caaaagtatg gacgacaagc
     5521 ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca aagcctatca aaaattgcag
     5581 gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc cttcatgcgg aacccattcg
     5641 atttatgacg gaagtttttc cggaataaaa aaaatggtgc cggagtggct actgcttatt
     5701 ttattcagca aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc
     5761 agaatggaaa tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta
     5821 ttgacagaaa aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt
     5881 gagatcgcgg aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca
     5941 agcaagatct tggaggatta tgaaaaaaaa ctccatcttt tttctaatta tattgttcga
     6001 gaacaggtat tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa
     6061 ctacttgaat atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa
     6121 atatggcatt tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa
     6181 aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac
     6241 tagaagccga tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg
     6301 cggtaggggc agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg
     6361 atgaagaatt gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa
     6421 taccaactgt tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta
     6481 aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg
     6541 tatatcgacc agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg
     6601 tatttgatat gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag
     6661 caaaagaaaa gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg
     6721 aaacattgat ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc
     6781 ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc
     6841 aattaggtat tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag
     6901 cactatcgat ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat
     6961 tgactgactt agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg
     7021 atatgctgac attgattgaa aaagcgcagc aagactacga cgagaaaaaa gcggaagaac
     7081 ttgcacagaa aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc
     7141 aagtgatggg tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag
     7201 tccctggaat cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg
     7261 tttattctat gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc
     7321 gccgaattgc tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat
     7381 tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta
     7441 tggaccaaat gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa
     7501 tgatcaagaa aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg
     7561 gtacagactg aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta
     7621 atacaattac tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag
     7681 aaagtatgcg gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc
     7741 tgttccgaaa attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa
     7801 ataaaagtga agcattcgat gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt
     7861 atggagaaac aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa
     7921 aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca
     7981 gtttttcaga gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt
     8041 cacaagaaga agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga
     8101 aaagaggatt taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga
     8161 tcatcaatga tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa
     8221 agggcgaata aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt
     8281 tggcaacgaa aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacta
     8341 cttatgaagc tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg
     8401 aagcaaaaaa atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt
     8461 ccacccacgc acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt
     8521 ttctaaaaaa tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat
     8581 tgatcaaacc gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg
     8641 tattattagg ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa
     8701 tgctagacac gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt
     8761 aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat
     8821 taccgttatg ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc
     8881 gtttggaaga aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca
     8941 acgtttccga tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat
     9001 tggcgtagat ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact
     9061 aaacctggtt tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca
     9121 tttttcaata ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct
     9181 agtcccctca cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg
     9241 agatcctcat tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca
     9301 ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta
     9361 ccaataaaaa atccaatttt ttttgtcata cgatttttcc tccttcaaac tatctataat
     9421 aatcttacaa aagataagta actggagcaa ataaaaacac ttactgacaa aagctacata
     9481 gaagatagat tacttatttt taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa
     9541 aatcgaattt aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa aaactgttac
     9601 actacatctt gtgaataaaa caatggaggt ggaaataaat aatggcagtt aaaatccgtt
     9661 taaaacgtat gggttctaaa aagagtccgt tttaccgtat cgtcgtagct gattcacgtt
     9721 ctcctcgtga tggacgtttc atcgaaactg taggtactta caatcctttg aaagaccctg
     9781 cagaagtagt tttaaaagaa gacttagttc ttgactggtt atcaaaaggt gcacaacctt
     9841 ctgacactgt acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta
     9901 aattctcaaa gaaataaggt gaccggatat gaaagattta agcgacttag tcttaacaat
     9961 cgttcgtccg ctagtcactt atcctgatca agtcaagtta gacgtcgtag agtcgaaaga
    10021 cttttacgag tacaatctaa cagtggctcc tgaagatatt ggccgtatca ttggaaaaca
    10081 agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt gttcggatca atgcaccgaa
    10141 aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa
    10201 ttttcgagtg cagctttttt gtttttttta ttaattgaag aaatgattat ttggcaaaac
    10261 aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca gccaatgaga aagaaaatgt
    10321 tatttattcc atttgctcta ttgttaagcg cttgtagttc ggttactcaa gaatcagaca
    10381 atacacatct atcaagcagt atatctgaaa ctgttaccag tccagaaaaa agtagtacca
    10441 aaacggcttc caccactgaa caaacaatgg ctacctcaaa taatgagaaa aagactgctt
    10501 tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc tctagatgta ccagtctctt
    10561 caggctatct caatatcgca gcgacacata caaagcaagg ttattctatt ttatattaca
    10621 gaacagatcg tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa
    10681 cctatctata tcaatatggg tttgcttcta gtcaagaaac aatccaagtt ttgcagccat
    10741 ttgagatcga cacaaatggt cagcaagtgg acttaggttc tcgaatcaca gggtatcagc
    10801 aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg aaactggcgt attcgaatcc
    10861 gtgggaataa tatagaaggc caagatccct tactattagc taaagaaatc gtagcttatt
    10921 tggaagaaaa cagtctacca gcacctgaac aatttggtaa aattactgtt gatatgggtg
    10981 atacgacgaa tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca
    11041 ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc
    11101 agcgcgctga tctgagccca tcatttaact ccaagtcaca ggaagtaatc aaaaatagca
    11161 cgccgcattt ttgcataatt ttggcttaat accttaaaag aaaactagaa ttgcctcttc
    11221 acacatgcta aaataaaaca gacaaaaaag aaatggggat ataagttgac tgaatacttg
    11281 aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca
    11341 acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc taacactatt ccaagacgga
    11401 aaagcacctg tggaattaac agtcaaaagt catcgcaaac ataaaaattt tgacttattg
    11461 agttttgaag gacatccttc tatcaatgat gtggaaaaat atcgggatgg catattaaaa
    11521 gtgtcaaaag acaactctgt agatctagct gaaaatgaat tctattacca tgagatcatc
    11581 ggtctgcatg tattagaaga tggaactgag ttaggtaaag taaaagaaat cttgtcacca
    11641 ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat
    11701 atcgcttctg tcgtcaaaga agtcgattta gaagctggag tggtccgtgt agaaattcca
    11761 gaaggactga ttgacgatga agattgatgt attgacttta tttccaagaa tgtttgaagg
    11821 tcctatgggg gaatccatca tcggtaaggc tgtagataaa ggattattag atatcaatat
    11881 ctctaacttc cgcgattatt cagataacaa acatcagaca gtagatgatt atccatacgg
    11941 cggtggagca ggtatgctgc tcaaagtcca gcccatctat gataacatcc aagccattga
    12001 aaaagctgca ccggatgtca aaaaaagagt aattcttctt gacccagctg gtaaacgatt
    12061 cgatcagaaa atggcagaag aattttcaca agaagaacac ttagtattca tttgtggtca
    12121 ttatgaggga tatgatgaac gtatccgcac attagtgaca gatgaagttt ccttaggaga
    12181 ttatgtcctt actgggggag aattaggtgc gatggtcatg atcgatgcaa cagttcggct
    12241 tttgccagat gtgttaggga atcagacttc agctcaaacg gattcttatt caaccggttt
    12301 attggaacat ccccaatata ctcgcccggc agaatataaa gggatgaaag taccagaagt
    12361 attgacaaat ggaaatcata aattgattga agaatggcaa ttaaaagaat cactaagaag
    12421 gacctatcaa cgtcggcctg atcttttgga aacacttgaa tggacacctc aaatgacgaa
    12481 atttttagaa gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag ccaagtgact
    12541 ttttctaaaa tcttctttac agtaggttga taatatgtta gtattttatg gtgagtgcaa
    12601 aagcacttaa ctattacgat attccgctgt gagaaaagct cataagaata tttggaataa
    12661 ggagaagaaa accatgaatc cattaatcga agaattaaca aaagaacaat tacgttctga
    12721 catcccagcg ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac
    12781 tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag
    12841 cgaaacttat acagtacgta aagtttctaa cggagtgggc gttgaacgta cattcccatt
    12901 gcatacacca cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa
    12961 attgtactac ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta
    13021 atcatcgtta tcagactgag aaagaaccct tggtattcaa gggttctttt tttgttctct
    13081 gtttttttat attaacgatt tctatttggg acatttttga ttaataaaaa ggggcaaaaa
    13141 aggggcaaca agttacagtt tttctatatc ttctttcaat ttttctttta ctccctttgt
    13201 cacatgcaaa taaatctttt cagtgatatc actattttcg tgcccaacgc gatcctgaat
    13261 agcatacaga ggagttccta gttccgctaa ttttgaaata tgggtatgtc taaaaatatg
    13321 tgaactaagt tttttatcaa tcttcatgtc agctttatga tttctcaaat aggtgttaat
    13381 tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat agaaattgac cattcggatt
    13441 taattctaga agctcattgt aaatagctat cgcttttttt ggtaaatcaa tttctcgcat
    13501 tccagcagca gtttttgtag aatcagattt tttcatatca gctattgaac gttctctata
    13561 catcatcgtc ccatttataa ctactgatgc attattattg gtaatgtgta catcgtcttt
    13621 acttaatgcg attgcttctc caggcctcat tccagtcaaa tatagccact ggaaaaggag
    13681 agaatatctt ttattgtgtg aagttgtgaa atttactagt cttttatatt catcatcttc
    13741 taaaaactta tctttaattt ttatcgtttt tgactctcgt ttataatcaa taactacttc
    13801 gttgatagga tttttttcta catagccttt tttcatagcg tatgaaaaaa gaagattcaa
    13861 tttggattta attacactga cgtacttatt tgacaagtca tctttatata tcatatcttc
    13921 aaaaatatta tttaaatcta ctgtattaat accagaaacg atgtaagtct caggtatctt
    13981 tttctttata gtgtttaaga tattattcgt aggataatat gtagactcct taacttgtct
    14041 tttgtaaata actaaccatt cttcgacaag ctcatgaaat gttagatcag gcttctgaag
    14101 tgttttttcg tttaatttta tatcaatttt tttatttaac tctagcaatg ccactttctg
    14161 agtttctcga gatttatttt tataagtaat gctcactttt tttctttttc tagtttttgg
    14221 atcaatatat cgctcattat atttatatac tttttttccg tttttatcag ttttggtttc
    14281 aatccacatt gttttcatct cctatttgtt gctataatag gaatggataa gtaagccaat
    14341 tatagcaggt ttatttttca tcacgtccac aaactttggc gagggagggg gcgtgttttt
    14401 ttgtttagta gctacctttc catgaagtat ctgcgaagaa cacttcaaat gttgttccat
    14461 catttttcac ttcaaaatat gctgttcctt gtgcggattt acccggttgg atggtttccg
    14521 aaaagtaatc tctactagct aaatcagatt taatatctga tgaatcataa aattctaaat
    14581 aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc tacaagaaaa tctacctttg
    14641 cgtaaaatag gccttctggt ttatagaaat catcaccgta atcttttgat acagactgga
    14701 cagttacgtt aatagagtta ccattttcag aagtaaaagg aactgcttct cctatactat
    14761 gtgcaactga tgccgtctgg ggttctgaag acgaagttgt agttgtagtt gtagtagagc
    14821 ttgaatcttt ggattttcca tctttatcta agaaagttcg ttgattataa tatacaacct
    14881 gttcagtttt tggactaacc caaattaaca ttgtttcatc atcagaatca gttgaataag
    14941 tatattggag catctctaat ttttttatat cttttgcact atttccatgt tcaattaatt
    15001 cagagtgctc tttataataa tctccttgtg catcctttga aatagataca attaattcat
    15061 cccgtgtgat agcattgtct aattctttgt aaacaaaatc atttgtccat ttacgtaacg
    15121 gtgaaccaag tgtctgataa acttgagtta aatcatcacc aacttttatt ttatgaacgt
    15181 ctgatgaagt tagttttttt gtttcatatt cagatgttga tgaaacagta ttttctttag
    15241 tgctagcaga attaggacta gtggagcatc cgcttaatac caagcctaaa aaaagcaacc
    15301 caaaaacaat ttttttcatt tttaattcct cacttctatg atatgatttt tttgtaggat
    15361 cttagaaata aggtttttag tccgtgttgc agcacggact ttttttacat tatataagaa
    15421 agcgataagc gctttctgga agtccgtaaa gattctttaa ttcctcaatt cgtttaggat
    15481 attgatcatt gtcttctttg tatagagaaa caatgagatt agcagcaaag caattagctt
    15541 cgctttcaga tttgcttcta gatgttcttg ttgatacata gtaactggat aagccacgat
    15601 gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa tctttcctca gagtctctca
    15661 gttcatcatt taagaagatt attgcacgac ctctaatttc ttgaaactgt cctttgggat
    15721 tctcgataaa aggaacgtat tgaattttaa tgcccatctt ttcacaaata taaaaaggat
    15781 tagcggactg gtatttccgc ttcaactcct cgactaaatt aatcgtatcc atctccataa
    15841 gctcacatct ttttgccttt ttctttgtct tctttcacaa tatcccagaa ggttccaata
    15901 aggatatctt ttacgcgttg gacctgttca ggcgtcagtg tttctccgcc ataagacata
    15961 ttgacatttg aatctagtaa tttatcaagt tcaactactt catcttttgt tgcccagtca
    16021 ggaacttggt ttctaccgag taaataatca gtagttacat taaaataatc tgctacaagt
    16081 tgtagtcttt tagtactagg agttctattt ttccattgat aaatcgtgtt tttaggtata
    16141 tttaattctt cttccaattg agttatactt atgcctcttt tatgagctaa ttcctttatt
    16201 ctatctagta aattcatttt cgcacctcac aagctacgaa aacactaata aaaaaattag
    16261 ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt tttcgtaagc taaaatatta
    16321 gctaaaaaga cttgataact aataaacact tcacggtcgg caaactctga aatgtaaatt
    16381 actaggcatt tctgtgtctt atttagctat gtctatatac taataaaaat attagttatt
    16441 gtcaacgaat attagctaat tttttagctt acagattatt tttttagaaa ggagctattt
    16501 ttatgtctga gaatttagac ttaaaaattc gagcggagat gagaaaaaga agaatgactt
    16561 tcaaagaact agctgcgctt atcggtattt caggagctta tttatcagat attctaaacg
    16621 gcaatcgtga tggaaagaaa gcacaacagc atatcgaaac agtgaaaaaa atattggaca
    16681 tccgataggg gggtaggcga tgacaaaact aaaaaaacaa gattttgtaa aaaaatacaa
    16741 ttattctcca tctacttatc aacgtcgaat gtcggaacta aaaaatacag caattttctc
    16801 agcggcgtat gaacgggtca caggacaaga agtttggatc aatacagaat tatacgataa
    16861 atttttgtct ttcaaatcct ataacaggtt acgcacaaga aaggtaacac ctaaagaatt
    16921 tatcgagaag catttagttg atttataaaa aacagaacat tttgagaggt gaaggttaat
    16981 gggtaaattc aacagagcat tggtattcag cgcaccgcta atcatctacg ctttaggact
    17041 ttggggaagc aggcaagcgt tgataggaac gattgtttac atggtttgga tttttatggg
    17101 gcttgatgaa gctgaagctg agtacagagc gaaaaagcca accgaggggg ctgactaaaa
    17161 atgaatagaa aagagaaact agaatgtata ttactattac tcagtttaat tctttcacta
    17221 atttctctat tgggaagttt gtatttttga tatcaacaat tttgtggatt tgggttctat
    17281 ttagagtaaa tatcattttt gtttggtgat taaggagttt attaaaatta attttcccta
    17341 cttcaaatgc aaggtaacaa gatatagcac ttttaggagg aatatttagc ggtacagctt
    17401 cagagtatat tctattatca tcgactgatc ctcctttagc aattaatttc ttaaatggag
    17461 tagctgacca tctgcggttt aagttagcgg atgaaagttc taaattaact aacatagctg
    17521 gttctgttga atagttagaa atgattactt ttgtataaat catatctgca gcgaagtaag
    17581 aagcattaag ttcaacttct agttgagggc gcattccttt tatttttatc cctgttaaaa
    17641 acgtactcaa tataaaactt attattgaca tccattgaaa aattgttaag tgaaaaaaat
    17701 tcaaaataaa caccaccagt ttttaactaa attataccaa aaaggagaga agaaataatg
    17761 caagaattag taattttgaa aaataaagaa gctgtgacta cgagcttaca agtggcagaa
    17821 agcttcgaga aaaaacatca acatgtttta agagatattg atgcactaaa aaaagatgtg
    17881 tccaattttg gacagatgtt tgtagaaggt aatgaaccag attcatatgg cagaaatcga
    17941 cgagttttct tcattagtag agatggattt ttcttgctgg ctatggggtt tacaggaaag
    18001 aaagctatct acttcaaaca aaaatacatt gaagcattca acgaaatgga agatgttatt
    18061 cgcaagaata ctgttcctca aacaattgaa gatatgatga tctatcaatt agaagaaatg
    18121 aaagatgtta aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa
    18181 gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg gagttctagg gggaaaagaa
    18241 agccgagctt atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa
    18301 cgtacctttt caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt
    18361 tcatttattg aaatgtggtt gccagaaact gcgatccgta tggaaattga tcaactgaac
    18421 agacaacaga gactttttgg tgatgacaat gaatagagct gaagcgctaa gaatagggac
    18481 ggcaattgct aattgctggt ggaaatacta taaaccaatc atcctaagcc aacaacatat
    18541 tgacaagcaa aaagcatggc aacaaataaa aaagtgactc cgccggcaag caaagagtca
    18601 caaagaaaac acatcataag gagattttag catatggaaa aagaactttc cactctagat
    18661 caatatttga ttgatcctga ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa
    18721 atcagacgaa atcttttgac gaatgaagaa ctagcttggg atcaagatga tttaggcaac
    18781 catgtaacta tttgggatca tgtttatctt atccatctat cgaagcattc gaataaacct
    18841 gaatatattt acgtcatcga agatggcttg attgatgcgc tagaagagta cgacagagat
    18901 aacttgattg atatctctta ttacggacca ggtaagaaat acattgctga aatggaggca
    18961 gaatttgatg agtgaaatca aagggacaac gaactttgaa aaacttttta gtcgtaagtt
    19021 aaataaaatt ctcaagaaaa aaggaaattt tgattattta tcttgggctc acgcgtggga
    19081 gattatgaaa aagaatgatc cacaggcaac ggtaactatt aatgagtaca aacactacag
    19141 ggttgtttct ggaactcatc aagactttct tgttgaggaa tataaacctt ttcttatgga
    19201 tgaaactggg acttatgtat ctgtctcagt aacggttaaa ggacacacgg aaaccga
//
LOCUS       DAZUMB010000043        18853 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000043 DAZUMB010000000
VERSION     DAZUMB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.043"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACG80L_001351"
     CDS             complement(<1..703)
                     /locus_tag="ACG80L_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023592.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACG80L_001352"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACG80L_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACG80L_001353"
     CDS             1758..2837
                     /locus_tag="ACG80L_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5023593.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACG80L_001354"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACG80L_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACG80L_001355"
     CDS             complement(3572..3799)
                     /locus_tag="ACG80L_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5023594.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACG80L_001356"
     CDS             complement(3902..6490)
                     /locus_tag="ACG80L_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS5023595.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACG80L_001357"
     CDS             complement(6510..7169)
                     /locus_tag="ACG80L_001357"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5023596.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACG80L_001358"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACG80L_001358"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS5023597.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACG80L_001359"
     CDS             complement(7942..8808)
                     /locus_tag="ACG80L_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5023598.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACG80L_001360"
     CDS             8917..9558
                     /locus_tag="ACG80L_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS5023599.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACG80L_001361"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACG80L_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS5023600.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACG80L_001362"
     CDS             complement(10999..11898)
                     /locus_tag="ACG80L_001362"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5023601.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACG80L_001363"
     CDS             complement(11900..12697)
                     /locus_tag="ACG80L_001363"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS5023602.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACG80L_001364"
     CDS             complement(12675..13379)
                     /locus_tag="ACG80L_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS5023603.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACG80L_001365"
     CDS             13510..14085
                     /locus_tag="ACG80L_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS5023604.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACG80L_001366"
     CDS             14203..14880
                     /locus_tag="ACG80L_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS5023605.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACG80L_001367"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACG80L_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS5023606.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACG80L_001368"
     CDS             complement(16943..17941)
                     /locus_tag="ACG80L_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS5023607.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACG80L_001369"
     CDS             complement(18167..18820)
                     /locus_tag="ACG80L_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS5023608.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5428 a         4087 c         3294 g         6044 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aat
//
LOCUS       DAZUMB010000044        18741 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000044 DAZUMB010000000
VERSION     DAZUMB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.044"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..532
                     /locus_tag="ACG80L_001370"
     CDS             257..532
                     /locus_tag="ACG80L_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023609.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="ACG80L_001371"
     CDS             545..2617
                     /locus_tag="ACG80L_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5023610.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2785..4668
                     /locus_tag="ACG80L_001372"
     CDS             2785..4668
                     /locus_tag="ACG80L_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5023611.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4680..5627
                     /locus_tag="ACG80L_001373"
     CDS             4680..5627
                     /locus_tag="ACG80L_001373"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5023612.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="ACG80L_001374"
     CDS             5647..6174
                     /locus_tag="ACG80L_001374"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5023613.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="ACG80L_001375"
     CDS             complement(6237..6890)
                     /locus_tag="ACG80L_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS5023614.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="ACG80L_001376"
     CDS             7022..7864
                     /locus_tag="ACG80L_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5023615.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="ACG80L_001377"
     CDS             8022..8918
                     /locus_tag="ACG80L_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5023616.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="ACG80L_001378"
     CDS             8921..9634
                     /locus_tag="ACG80L_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS5023617.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="ACG80L_001379"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="ACG80L_001379"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5023618.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="ACG80L_001380"
     CDS             10169..10396
                     /locus_tag="ACG80L_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS5023619.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="ACG80L_001381"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="ACG80L_001381"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5023620.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11724)
                     /locus_tag="ACG80L_001382"
     CDS             complement(11182..11724)
                     /locus_tag="ACG80L_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5023621.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="ACG80L_001383"
     CDS             complement(11961..12359)
                     /locus_tag="ACG80L_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5023622.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="ACG80L_001384"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="ACG80L_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS5023623.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="ACG80L_001385"
     CDS             13472..14239
                     /locus_tag="ACG80L_001385"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS5023624.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="ACG80L_001386"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="ACG80L_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS5023625.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="ACG80L_001387"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="ACG80L_001387"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS5023626.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="ACG80L_001388"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="ACG80L_001388"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS5023627.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5093 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcgttttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacatcgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt t
//
LOCUS       DAZUMB010000045        18686 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000045 DAZUMB010000000
VERSION     DAZUMB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.045"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..569
                     /locus_tag="ACG80L_001389"
     CDS             105..569
                     /locus_tag="ACG80L_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5023628.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            553..1182
                     /locus_tag="ACG80L_001390"
     CDS             553..1182
                     /locus_tag="ACG80L_001390"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5023629.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1536..3014
                     /gene="msr(C)"
                     /locus_tag="ACG80L_001391"
     CDS             1536..3014
                     /gene="msr(C)"
                     /locus_tag="ACG80L_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5023630.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3061..3507
                     /locus_tag="ACG80L_001392"
     CDS             3061..3507
                     /locus_tag="ACG80L_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5023631.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3706..4221)
                     /locus_tag="ACG80L_001393"
     CDS             complement(3706..4221)
                     /locus_tag="ACG80L_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5023632.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4338..5540
                     /locus_tag="ACG80L_001394"
     CDS             4338..5540
                     /locus_tag="ACG80L_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5023633.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            6155..6481
                     /locus_tag="ACG80L_001395"
     CDS             6155..6481
                     /locus_tag="ACG80L_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023634.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(6598..6942)
                     /locus_tag="ACG80L_001396"
     CDS             complement(6598..6942)
                     /locus_tag="ACG80L_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023635.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(7018..8265)
                     /locus_tag="ACG80L_001397"
     CDS             complement(7018..8265)
                     /locus_tag="ACG80L_001397"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS5023636.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(8262..9068)
                     /gene="proB"
                     /locus_tag="ACG80L_001398"
     CDS             complement(8262..9068)
                     /gene="proB"
                     /locus_tag="ACG80L_001398"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS5023637.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            9298..9825
                     /locus_tag="ACG80L_001399"
     CDS             9298..9825
                     /locus_tag="ACG80L_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS5023638.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9888..11258
                     /gene="radA"
                     /locus_tag="ACG80L_001400"
     CDS             9888..11258
                     /gene="radA"
                     /locus_tag="ACG80L_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS5023639.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            11292..12449
                     /locus_tag="ACG80L_001401"
     CDS             11292..12449
                     /locus_tag="ACG80L_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS5023640.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            12619..14079
                     /gene="gltX"
                     /locus_tag="ACG80L_001402"
     CDS             12619..14079
                     /gene="gltX"
                     /locus_tag="ACG80L_001402"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS5023641.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    14076..14315
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14401..14937
                     /gene="epsC"
                     /locus_tag="ACG80L_001403"
     CDS             14401..14937
                     /gene="epsC"
                     /locus_tag="ACG80L_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS5023642.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            14934..16346
                     /gene="cysS"
                     /locus_tag="ACG80L_001404"
     CDS             14934..16346
                     /gene="cysS"
                     /locus_tag="ACG80L_001404"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS5023643.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            16343..16738
                     /locus_tag="ACG80L_001405"
     CDS             16343..16738
                     /locus_tag="ACG80L_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS5023644.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            16731..17591
                     /gene="rlmB"
                     /locus_tag="ACG80L_001406"
     CDS             16731..17591
                     /gene="rlmB"
                     /locus_tag="ACG80L_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS5023645.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            17595..18125
                     /locus_tag="ACG80L_001407"
     CDS             17595..18125
                     /locus_tag="ACG80L_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS5023646.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            18202..>18686
                     /locus_tag="ACG80L_001408"
     CDS             18202..>18686
                     /locus_tag="ACG80L_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5023647.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     A"
BASE COUNT         6031 a         3370 c         4053 g         5232 t
ORIGIN      
        1 agcgtttttt ttattacaca gctgattctt tgactttttt ctttttttat tctattattg
       61 acaagtcaat cgttgacgag tcaattatta gaaaggagga aattatgtct ttagaagaga
      121 ttagtcacct tttatatcaa atcaaattag ccgatcaaaa aatcgtccag atctttgaag
      181 aaaatatcgg tataagtctt actagatacg agatcatgat gagactgaaa gaagaaaatc
      241 cattgctaca aagcagtttg caagaaaagt tgcagattga tcaaggagca gttactcgtc
      301 atctgaaggt attggaagca aatggctatg tttcgcgtca gagaaatcct cagaacaacc
      361 gggaagtgta tgtagcgcta acagaaaaag cccagaagaa aatcaagcat tgtgaaatgg
      421 accataaaga tacaggttcc gtgctatccc cggctttcag cgatgtagaa atgaaacaat
      481 ttctaacact tttagataaa ttgaataccc aactttgcaa tataacaaca gaaacgaaag
      541 aggttaataa taatggaaca aaaaaatgat ttttcaacaa ttatgactgg tagaagatcc
      601 gttcgcgtat atgatgaaac atacaaaatc ccacacgaag aaatgctgga aatgatcaaa
      661 gaagcgacga ctgcaccttc ttctgtcaat atgaaaccat ggcgctttgt ggtcgctgaa
      721 agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat
      781 acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata tggcgaagaa
      841 atctacaacc aagcttacga atcagggaag atgccaaaag aagtcagaga tcaacaatta
      901 gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga tgtagtcaaa
      961 gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca tggctatgaa
     1021 acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg tttggataaa
     1081 gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga aggttacgag
     1141 tctgttcgtt tagcaccaga aaagatcact atgttcgaat aaaaggtttt cagttactat
     1201 tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa
     1261 aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa
     1321 aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta
     1381 gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag
     1441 gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata
     1501 attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa
     1561 caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct
     1621 atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca
     1681 aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca
     1741 actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca
     1801 gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac
     1861 gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc
     1921 caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt
     1981 atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa
     2041 tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca
     2101 ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga
     2161 aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag
     2221 tgtccagtaa aaagaaaaag caacaaatca gactggatcg gttgtcttcc tctaaacaaa
     2281 aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa
     2341 aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat
     2401 ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg
     2461 ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg
     2521 tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag
     2581 gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga
     2641 catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg
     2701 tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta
     2761 cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta
     2821 atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag
     2881 agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg
     2941 tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta
     3001 cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga
     3061 ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc
     3121 ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg
     3181 attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg
     3241 ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt ccaaatgatc
     3301 ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt attcggtgtg
     3361 atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc ttggaaaatg
     3421 aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc aagaagtgtg
     3481 ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa ggccttggca
     3541 tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg aacgataggt
     3601 tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt tattgaagat
     3661 aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact caaaaaactg
     3721 ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa gcataattct
     3781 gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa
     3841 ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga atagcagtcc
     3901 agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa attcatcttt
     3961 ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac gtccttcatc
     4021 agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg tcacataacc
     4081 ctgctggtct aacttggcaa gcgttgtata gatctgactg tgatgggctt cccaaaaaat
     4141 tgtgatcagc tgcttcaatt catacccaga acgtggtttg gataaaagca cgcataataa
     4201 tatataggct aaagtattca taaaaatcct acctctcgtc ttattttctt tattatatca
     4261 cagtaaagtt tccacgtttt acatgtgcat attgacatgt aaaataaatc atgtatgatg
     4321 ctaatagaga gaaagggatg gaaatattgg agaatcaaat agatatacaa gaacaaacaa
     4381 atgaagaaac aaaggggaaa agttcagcgc tagttgtagc agctacctgt atgatcgctt
     4441 ttatgggagt gggtctagta gacccaatct taaaaacgat tgctgcacag ttaaacgcta
     4501 cgccagctga aacgaccctg ttatttacta gttacatgtt agtcactggt gtggtgatgc
     4561 tatttacagg tttcttatct agtcggatcg gtttgaaaaa aacattgatg acaggattgt
     4621 ttatcatcgt tgtattcgct ggtcttggcg gattatctgg gaatatcgga atgctgattg
     4681 ggttacgtgc cggctggggt gtcgggaatg ctttatttgt ctctactgct ttagcaacga
     4741 tcgtcagtat tttagttggt aacacagaaa aagcaatcat gatgtatgaa gcagcacttg
     4801 gctgcggaat ggctgttggc cctctagtcg gtggggtctt gggcagtggt tcatggcgtt
     4861 atccattttt tggggtagca tttttgatgt tttgcggttt tatagcccta gcggtacttt
     4921 tacctgaaac tgagaaacca aagagaaaag taggcctttg ggaaggtgtc aaagcgttaa
     4981 gcaatcataa acttcgttca gttggactaa tcgcattgtt atataatttc ggattcttta
     5041 ctttacttgc gtatgcgcca tttttattga ttggctattc tgaattagaa gtcggttttg
     5101 tattttttgg ctggggtgtg cttctggcta ttgcttcgat tttcgttgca ccggctcttg
     5161 agcggaagtt tacgacttat cggacaattt tagcagcatt tgtcttattc attatttgtt
     5221 tgctgttatt aggagctgga acttctatgc cagtccttgt accgatcagc attgtgttag
     5281 caggattttt ccaaggaatt atcaatacac tgttgacgac gattgctatg gaaataccag
     5341 gtctgcaacg aaacgttgct tcgtccagct atagttttgt ccgctttttc ggtggagctt
     5401 tagcaccatt tattgcaggt gtgattggtg aacatattgg tgaaagctat gcgttttatt
     5461 ttgcaggtgc cattgttctt atcagtgtat gttgtatcat ttatcaccgt aattattttg
     5521 ctacaaatga agaagaataa gaaatcagag gttgtgaaaa agctgtcctg catcaagtca
     5581 taagaacagc caaacaaaaa tagcttctca tatttttcgt ttggttgggc ttattaccgc
     5641 agatgcaagc ttttgaacac cgtttatcag gaggttgtaa aatcagcgtt ttgtcctgag
     5701 tattagagaa acaaacggaa aaatagttcc tcatattttg ccgttttttg agctaatacc
     5761 gaaggacagc tgattgaaca ccgtttaaca gaggttgtaa aatcagcgtt ttatcctaag
     5821 tattagagaa acaaaacgga aaaatagctt ctcatatttt accgtttttt gagctaatac
     5881 cgagggtcag ctgattgaac gccgtttacc aagaggttgt gagactgtcg tttaactctg
     5941 aatcacaagg aacaatttca aaaaacagtt tctcatgttt gttgaatatt gtgacttatg
     6001 attgaggagt tggcagaaga acaccattta ttcggaaaaa ggggatgtga cacgattttt
     6061 gtcacattcc ctttttttct caaaataatg aaactggaaa attttcagta ggtaagctaa
     6121 aattcattta agaaaaagaa tggggggata atagatgctg gaagaatatt ttcaggaagt
     6181 cgggttgaca ctcgttgaat tacccattga attttcagat gatcggttga tttatcgtgg
     6241 ctatatcaaa gaattgccaa taatcaatgc ggaaggtgaa actagaaaag cgatgcagca
     6301 aaaaatagct ggtatgtatc aggtgtatcg ggaacagcta ctggctgaat cagcggaaga
     6361 agaaaaagaa aaaacgatga acttgtccat ggatgagctg cttcgttatt atgatggcga
     6421 aacatttgac ggattttcgc ttcttaacga agaggaaaca gatttaccag gcagctggta
     6481 ggcgaacgcc gtttatatgg aaaaagaggc tgtgacaaaa tctttgtcac agcctctaaa
     6541 aagtccggca gttttttgcc gatcttcaag gcactctact tatcgtacgg atatttttta
     6601 gcggaaagtc cgattcaagt ggtttttatt cagctgatat ttataagata agtgaaaaat
     6661 cccagcaatc agtagcgtaa tcgtataagc gattgaatga tcgaaaaaag aatagtaatt
     6721 gattgtcgct aataagcata atgcaaccat gtaactaatg tgcaagcgtg taaccagccc
     6781 tttttgcccg attttgtcaa aatactgcga ccatttttcc cgttgactcc ctatgcttcg
     6841 ttttttgaat tgaaagaaaa gggataaaat accgactccc agaaaagcag cgatattcag
     6901 agaaataagg taaataaaaa aaatcattaa aatttcaaac atattgacac ctttctagtt
     6961 ggatagaacc tttacagaaa aaaggcaaaa ggcgggaata atgtttcaca cgaaacatta
     7021 ttcgcggatt tgtccatcgc cataaatgat atattttgtg gaagttaatt catttaaccc
     7081 cattggacca cgggcatgca acttttgagt agagatacca atctctgcac caaaaccaaa
     7141 taccgatccg tctgtaaaac gagtggaggc attgacatag acagccgccg cgtccacttc
     7201 atttaagaaa cgttgactag ccgcataact atctgtcacg atcgtttctg aatgtttagt
     7261 attatgccga ttgatatgtg caattgcttc atccaaagaa tcgaccactt tgacagcaag
     7321 gatataatct agatattcag tatcccaatc tgtgtcttct gctaaagcag ccgtctctag
     7381 atattctaac gcacgagcat ccgcacgtaa agagaccccg tgttctacca gttctttttc
     7441 aatagctggc aagaaaaaag gtgcgatctc agaatggatc agcaatgttt ctgccgcgtt
     7501 acagacagat ggacgggaag cttttgcatt gacgatgatg ttcactgcca tagataactg
     7561 tgcttcttta tcgatatata cgtgattatt tcctgtacct gtttcaatga taggtaccgt
     7621 tgcctgttct ttgactcgtt ggattaattt tgctccgcct cgaggaatca gcacatctaa
     7681 gtattctgtc aatcgcatca actgtcctgc agtttcatga gaagtatcat cgacaaactg
     7741 gatggcataa ggagattcgc cagcactgac cagtacttgc tgcaataatt ggactaataa
     7801 ttgattcgag aagaatgctt ctttgccacc gcgaaggata acggcattcc ctgatttgaa
     7861 tgctagacta gctgcatctg tcgtgacatt tggacgagat tcgtaaatga tgccgatcac
     7921 gcctagtggc acgcgctttt tgccgattct caatccgtcg gtgttcgtcc acatctcatc
     7981 tacttttcca ataggatcgt ctaacgaagc aattttttcg atctctgtag ccatggcatt
     8041 gattcgctca ggtgtcagct gcaagcggtc ttgcatcgtt tcactgatat tgttttcgac
     8101 tgctttttct aagtctttat gatttgcttc aatgatcgct tgttgattct ttttgatggt
     8161 ttgtgccatt tgaacaagta gctcattttt gcggtttgta ggcataagtg ctagttgttg
     8221 cgcgctatct tttgcctgtt gtcctaattg catcaagtca gtcatttcaa gtcctcctta
     8281 aataaagtac ctacttcttt tcctgcaaga atatcaaaaa taattgccgg attttttcca
     8341 tttgctaaaa tcattgcgct gtttgcttca aatatcctag aagcggcttt taatttactc
     8401 tgcataccgc ctgttccaaa tgtgctacca gcaccacctg cttgtgccag aatcttttca
     8461 tcgattttat cgatatagcg atacatctga gcctcgggat ttgtagtcgg attatctgaa
     8521 taaaacccgt cgatatcaga aaggacgatc aaaaggtctg ccgcgacaag ttttgccatg
     8581 atggctgaca gctgatcatt atcgccaaat tttgtcagat gatctaattc atccactgct
     8641 acagtatcgt tttcgttcac gatagggatc gtgtccataa tcaaaagctg ttccaatgta
     8701 ttcctcacat ttttacgact ttcaggataa tctaccacgt ctcttgtcac gaggatctgt
     8761 gccgtctgtt gattatatac actgaatctt tgattataga tattcatcag ttcggcttga
     8821 ccaacagccg ccgcagcttg ctgttcagga atcaaagtcg gacgttctga caaatgtaat
     8881 ttatgcatac caacgcctac agctcctgat gagactaaga tcacttcctt tccccgattt
     8941 ctaagatcag aaaggacaaa agctaactga tcgattgccg caagattgat atttccattg
     9001 ggataaatca atgtacttgt tcctactttg acaataattc gttttgcatt tttaatttct
     9061 ttacgcatgt gacacctcaa tcaaatatcc actcattaat ttcgttctat tctacttgtt
     9121 ttgacgagta aagtcatgta aaaaatagtt tttttgtaca acttgcagtc gtttggaaaa
     9181 agaacgaatt aatggtttac tttaagatag cagaatttct tgaagaattt gtaaagaaag
     9241 tcttttacag cctcggatat gttagaatag gagtagttta tttagggagg aaaaccggtg
     9301 agaaaaagag gatttgaagt tatttctaaa tataaagatc aaaatatcaa tctaccgcag
     9361 cgcgctacac atcatgctgc tggctatgat tttgaagcag cagaagaggt cgtgattcca
     9421 agtatctgga aagcaggctg gaaaaagggg ttggaaacag tcgcagatgg attgaaaaaa
     9481 gatgtatcag aaaatgaaaa acaattgaaa ccaaccctcg tgcctactgg aatcaaagct
     9541 tacatgggag aagatgaatt tttgcagctt gctaaccgtt caagcaatcc gttgaagcgg
     9601 tttttagtgt tgacaaacgg agtaggtgtg attgacagtg attattacaa taatgaggca
     9661 aatgaaggcc atatcatgtt ccaattcacc aactttggtt tcaaggatgt agtgatcaaa
     9721 aaaggcgagc gtatcggcca agggatcttt ttgccgtttt taaaagcaga tcaagatcaa
     9781 acgactgctc aacgtcaagg cggatttggt tcttccagta aataaaacgt taaagatact
     9841 taagcttgat ttaatgtctc atgattagga taaaaggagg cggtacaatg gcaaaaaaat
     9901 caaaagtaca atttgtttgc caaaattgcg ggtatgtgtc gcctaagttc ttgggacgct
     9961 gcccaaattg cggaaaatgg aatacgatgg tcgaagaaat cgaacaagat acaacggacc
    10021 gcagaactcg gacaagtttg acaggagaaa aagcaaaacc aacaaaaatc gcagacgtcg
    10081 tccctaaaaa agaacctcgg atcaaaacaa aattagaaga actgaaccgt gtgttaggcg
    10141 gaggtgtggt gccagggtct atggtcttga ttggtggcga tccaggtatc gggaaatcca
    10201 ctttgctgtt acaagtttct cagcaattag cagccatagg cggaaaagtc ttatatgtat
    10261 ccggcgaaga aagcgcagag cagataaaat tacgcgcgga acgcctcgga agtatcaaca
    10321 cagaatttta tctatacgct gaaacagata tgaacgaaat cagtcgtgcc atcgaacata
    10381 tctcccctga ttatgttatc attgattcca tccaaacgat gacgcagcca gacatcacaa
    10441 gtgtggcagg aagtgtcagc caagtaagag aaaccactgc agaactactc aaaaccgcta
    10501 aaacaaatgg gatagccatt tttatcgtcg gtcatgttac caaagaaggc tcgatcgccg
    10561 gcccaagaat gttagaacat atggtggata cggtgttata ttttgaaggt gaacaacatc
    10621 atagttttcg gattttgcga gcagtaaaaa accgatttgg ctcaacgaat gagattggga
    10681 tttttgaaat gcatgagcat ggattggaag aagtcgctaa tccttcacag attttcttag
    10741 aagaacgcct agacggtgca actggctctg ctatcgttgt ggcaatggaa gggacacgtc
    10801 caattttggt agagatccaa gcattagtca cacctactat gttcggaaat gccaaacgaa
    10861 caacaacagg tcttgatttc aatcgggtat ctctgatcat ggcagtgctg gaaaaaagag
    10921 ctgggttgct tcttcagaat caagatgctt acttaaaagc tgcaggtggg gtaaaattaa
    10981 atgaaccagc gattgattta gctatcgctg tcagtattgc ttcaagctat aaagaaaaag
    11041 gaacacagcc aacggaatgt ttcatcggtg aaattggact gactggagaa atcagacgtg
    11101 tcagtcatat tgaacaacga gtcaaagaag tccaaaaact cggcttcaca aaagtatacc
    11161 tacctaaaaa caacttaggt aactgggaag cgccaaaagg aatcgagata gttggagtag
    11221 caactttggc tgaaacgttg aagcgtgtgt tccgctaact aataaatgat tgtcgaaacg
    11281 gaggaaaaac gatgcaaaaa cgtgtcatta ctttgctgat gatcattgtc ggcgcaagct
    11341 tgggtatttc atttttaccc tcagcttggc aggcaatcgg tcaacagtca aacggatggc
    11401 tgaataatac atttaccaac agcctgttag gcgcacttat tttcttttta ttatctttgc
    11461 tgttggccaa acatatctca gctattatca aacaagtaga acaaaaacta agtgaggtca
    11521 gcttgactta tcttctcttc ggtgcgatcg gtgcgatcat cggattgact ttaggtgttg
    11581 tgatctcgac ccctctttac aatatggaaa ttccattcgt caatagtgta ttgccgattt
    11641 tactgatgat catcttaggc tatctgggat tgagaatggg aacgacacga atcgaagaat
    11701 ggaagaaagt ttttggttca cgaagcaaaa aaatcgagca agaaacagct agtcaagttg
    11761 agagtcaact gttagaacga aaatcaggag aaaatttcca taaatataaa atcttggata
    11821 caagcgtgat cattgacggt cggatttatg atattacaaa gactggattc ttagaaggaa
    11881 ccttgatgat ccctaatttt gttctttacg aactacaata catcgcggac tcaggggata
    11941 gtttgaaacg tgtgcgtggt cgtcgcggat tagatatcct aaatgccttg caaaaagaag
    12001 agggtatatc tgtcgaaatg tacgatggag actttgaaga tatcagtgaa gtagacagca
    12061 aattgatcaa attggctaag cttttagatg gtgtcattgt aaccaatgac tataacttga
    12121 ataaagtctc tgaattccaa aatgtacctg ttcttaatat caatgcgttg gcaaatgccg
    12181 taaaaccagt cgtcatccct ggtgaaacga tgaatgttct agtagtaaaa gccgggacag
    12241 aacgccaaca aggagtagcc taccttgatg acggcacgat ggttgtcgta gaagatggac
    12301 agcattacat gaatgaacgt atcgaagtcg ttgtaacaag tgcccttcaa acagctgctg
    12361 gacgcatgat ctttgctaaa cctgcacatg caggacgagg aatcgacaat aaaaaagaga
    12421 cagagaaaaa gactaaacaa tcaaaataaa aaagaaattc agccaactgg taaggaaatt
    12481 caaccagttg gctttactat ttaccttaga aaaggtaaaa tttgtgctga gtggctttaa
    12541 ataaatgata tttgagaagc cgtaactgac ggaatttttg ctgttagtca aagtctagtt
    12601 tgaaagaaga ggaagaaaat gacgaaagta cgcgtacgct atgcaccaag tccaactggg
    12661 cacctgcata ttggaaatgc aagaactgca ttattcaatt atttgtttgc ccgccacaat
    12721 gatggagact ttattattcg tattgaggat actgaccaaa aacgtaatat cgaagatggc
    12781 gaaaaaagcc agttggaaaa ccttgcttgg ttgggaatga attgggatga atctcctgaa
    12841 aatcctggag aatacggccc ttaccgtcaa tctgaacgta aagaaatcta tcaaccgctg
    12901 attgatcagc tgcttgctag caatcgagca tacaaatgct attgcacaga agaagaatta
    12961 gaagcagaac gggaagcaca acgcgcaaga ggagaaatgc ctcattacgc aggaacatgt
    13021 gcgaacttga cacctgaaga acaagcagaa aaagaagcac aaggtctaga atctgttgta
    13081 cgtttccgtg tgcctcgcaa tacagaatac gcatttacag atatggtgaa aggcgctatt
    13141 tcgttcgaat ccgataatat cggtggcgac tttgtcatcc aaaaacgcga tggtatgcca
    13201 acttataatt ttgctgttgc ggtcgatgat catttgatga aaatcactca cgttttacgt
    13261 ggagatgacc atattgccaa tacaccaaaa caactgatga tctatgaagc atttggctgg
    13321 acaccaccaa ccttcggaca tatgacattg atcatcaaca gtgaaacagg taaaaaacta
    13381 agcaaacgtg acgaatcaat cttgcaattt atcgaacaat atcgcgaatt aggctatcta
    13441 ccagatgcaa tgttcaactt tattgcatta ttgggctggt cacctgtggg cgaagacgaa
    13501 atcttcgaac accaagaatt gatcaaaatg ttcgatccaa aacgcctaag taaatcaccc
    13561 gcagctttcg atgcgaaaaa actgcaatgg gtaaacaacc attacatcaa agcaatggat
    13621 ctagatgcat ttactgacat gtgtctgcct tatctaatcg ctgatggacg tgtagcagaa
    13681 aacccagatg aaaagacaat cgaatgggtg aagaagatcg ttagcctgta tcaacctcaa
    13741 atgagctatg ctgcagaaat cgttgatctg tctacgctat ttttcaatga acatcctgta
    13801 ttgaatgaag cagcaaaaga agtacttgct ggtgaaacag ttcctactgt tctccacgca
    13861 ttcaaagcac agctagaaca aatggaagta gtagatgctc caactgtcaa agctgccatc
    13921 aaagctgttc aaaaagaaac tggcgtaaaa ggaaaaaatc ttttcatgcc aatccgtgta
    13981 gctgtatcag gacaaatgca tggtccagaa ttgcctgaaa cgatcgaatt gcttggtcgt
    14041 gaaaaatcat tagcacattt aaatcaagta ctgaactaaa cacgatgaac agaaaaaagt
    14101 aagcagctga tcattacaag agagataccg ggtggtgcaa ggtatcaatg aatggttgcc
    14161 gaaatgcctc tgggagtggt cttggcgaaa cacagaaaac aggaaaaact gttttatagg
    14221 aaacaaagta gccaagaacg tataacaggc gttagctgtc agagataatc attgattatg
    14281 aagaaaagtg gaaccacgcg gaaagcgtct tttgataagg aaacttattt aaaaggcgct
    14341 tcttttcttt ataagaagat gatttcctac ataaaattct tacgaaagga gtggaaagtc
    14401 atgagttggt ttacccgagc gattgatgca gcaaagagaa acgatccggc tgcacgttcg
    14461 acagctgaga tcgttttgac ataccctggt tttcatgcct tgttttggca tcgtttctct
    14521 cattttttat atcagcaccg gctcttttta ttggcgaaga tcaatgctca attctggcgt
    14581 ttcctaactg gtgttgagat ccatccagga gcaacgattg gaactggcgt gttcatcgac
    14641 catggaatgg gaatcgtcat tggcgaaacc gcagaaatcg aggacgatgt gattttattc
    14701 catggcgtaa cattaggcgg cacaggaaaa gaaacgggta aaagacaccc aactgttaaa
    14761 caaggagcca tgctctctgc gaatgcgcag atattaggac cggtaacgat cggaaaaaac
    14821 gccaaaatcg gtgcaggtgc cgttgtgttg aaagatgttc cagacgacgc tacagcggta
    14881 ggagtaccag caaaagtcgt tcgaatcaaa ggagaaaaag tgaggagatc agaatgataa
    14941 aaatctacaa tacattgacc agaaaaaaag aagaattcat ccctatacaa aagggacatg
    15001 tcggtatgta tgtctgcgga ccgactgtgt ataattatat ccatatcgga aatgctcgta
    15061 gcaccattgc tttcgataca attcgacgct attttgaata tcgaggctat aaagtcgatt
    15121 tcgtatcgaa tttcacggat gtagacgata aaatcatccg tacagcaaac gaattaggta
    15181 ttacagcacc agaagttgct gatcgtttca tccgagcatt tgaagaagat acgaaagcgt
    15241 tgaatgtcga acctgctaca ctgcatccaa gagtcatgga tcatatgcca gatattttag
    15301 catttattca agtgttggtt gaaaaaggct atgcttatga atcttcaggt gatgtgtatt
    15361 accgtacccg taaatttgaa aattatggga aattaagtga ccaatccatc gatgaactgg
    15421 aagtcggagc aagtcagcgt acaggggaag aacagcagca aaaagaagac ccattagatt
    15481 tcgctttatg gaaacaagca aaacctggcg agatttcttg ggattcacca tggggcaaag
    15541 gacgtcccgg ttggcatatt gaatgttccg ttatggcaac aaaacattta ggagaaacga
    15601 tcgatatcca tggtggcggg caagatttag aatttcctca tcatgaaaat gaaatcgcac
    15661 aaagtgaagc gaaaacaggg aaaaaattcg ctaattactg gatgcacaat ggctatgtaa
    15721 cgattggcga agacgatgaa aaaatgagta aatctctagg gaatttcatc actgtacatg
    15781 aaatgatcaa aaacgttgat ccacaagtct tgcgtttctt catggcgacc acacaatacc
    15841 gtcgtccgat ccgctacagt gaaacgacga tgaaagaagc agccaccaat ttacaacgac
    15901 tgaagaatac gtttgacaat ttgaaattcc gccaagaaac tgcctcagag aatttggcag
    15961 atgatgcgga taaaatcgct ttgttagaaa atttagaaca gcgattcatc gaagaaatgg
    16021 acgatgattt taatgcagca aatgggatca cagtggtcta tgagcttgct aagtggctga
    16081 acacttatct agaccagccg aaagtttctg ccgctgtctt ggcacaagga gaagctttat
    16141 tctcgcaatg gctttcgatc ttcggtattt tatttacagc agaagagtta ttagatgaag
    16201 agattgaaca attgatcgaa gaacgaaatc aagcaagaag caatcgtgat tttgtccgaa
    16261 gcgacgaaat tcgtgatttg ttgaaagaaa aagggatctt attagaagat acaccgcaag
    16321 gaacccgatg gagaagagaa gcatgagaga ttacacacaa ttgaacggtt tagccttggc
    16381 ttatgttgga gatgcgattt acgaaatcta tgttcgtgac cacctgattg atcaaggaga
    16441 aacaaaaccc aataagctgc atcgtgcggc aacgaattat gtttctgcga atgcacaagc
    16501 ctttttgatt cagcaaatgc tggaagaaca tcttttgaca gaagaagaag aactttatta
    16561 tcgcagagga agaaatgcta aaagccatac gagcgcaaaa aatgctgatg ttacgacgta
    16621 tcgggtcgca acagggttcg aagcgctcat gggctatctg catttgacaa aacaaaccga
    16681 gcggatggaa gaattgatcc gctggtgtat tcagaaagtt ggtgaaaaaa atgggtaaag
    16741 aaagaacaaa caaacatcaa tcgaaacgtg gatttgaaga caaaaaacaa aatagatatt
    16801 caaataaaaa gcaaactgga cgaaatcagt cagtttctga tcaaaatgaa gaagcattag
    16861 aagataattt cgtctttggg caccatgccg cagtagaagc gctacaacaa gggcgaggga
    16921 ataaactctt tcttcaagaa gattcacgag gagaaaaagt agaagaactg aaagaaatcg
    16981 cacgtgaaca agctgtgcca gtcaaatggg ttcctaaaca aaaattagaa actttgagcg
    17041 atcacggcgt gcatcaagga gtcgttcttg cgattacacc ttatgaatat ttatcccttg
    17101 accaactgat tgaacgaaca aaagagaaaa aagaagaccc cttctttttg attttggata
    17161 gcatcgaaga tccacacaat tttgggtcga ttttacggac agcagacgca agtggcgtcg
    17221 atggtgtgat cataccaaaa caccgagcag ttggaatcac acctatcgta acaaagactt
    17281 ccactggggc agtcgaacat gtccctgttg cacgcgtgac gaatttggta caagctgtgc
    17341 aacagctcaa agaaacagga ttttgggtat ttggcacaga tatgaccggc acaaacttcc
    17401 aaaaatggaa tgccaaagga tcaattgctt tagtcattgg taatgaaggc aaaggaatga
    17461 gtccaggatt gaaaaaagaa atggatgaat tgctgacgat cccgatgacc gggcacgtcc
    17521 agagcttaaa tgccagtgtt gctgctggtt tgcttatgta tcaggctttt tcccaacgtc
    17581 agggggagta gaccatgaaa aaacagctgt tgattgtaga cggctacaac atgattggtt
    17641 cgtggccgga acttgtccag cttaaaaagc aggacaaaat ggcggatgca agagaagctc
    17701 ttttacatcg tttatcaaat tatgcgaaat atgaaggaat ggaagtcatc gttgtcttcg
    17761 atgcccaact agttccgggc atccaacaaa attataagaa ataccagttg gatgttgttt
    17821 ttacgaaaga agacgaaacg gctgacagtt atatcgagag aatcgctggt cagctgaatg
    17881 accgtttgac acaagtgaca gtggcgacaa gcgatttagc agaacagtgg atgatctttt
    17941 cccaaggagc attacgaact tctgcttatg aattatataa gacgataaaa aaaacggaaa
    18001 aaacaatagc ccaagaaaat aaagatatgc attttattga tttccgacgt aattctccat
    18061 ggaatgaaga gcagttaaaa aaattgtccc aaaaattgga cgatttaatg agaaaaaata
    18121 aataacatat cgttctaatc agttcgatga atcattgatg ataagattcc tccataaacg
    18181 aatttatgga ggaattattt tatgtctgag gagttattgc gcgctgcacg taatggtgac
    18241 gaagaagcat ttgctgccct gtatcaaaaa tattttccac ttttactacg tttgaaaaac
    18301 acctattatt taagaaatta tgtttctgag gattgggaac aagaagcaag gatcgcttta
    18361 tatcaggcat tgagaactta cgaaaaaagc aaaggtgtta gctttggaag ttattataaa
    18421 agcgttataa gcaatcaaat ctattctctg ttgaggaagc agaacgcgct gaagcgtcgt
    18481 gatcaaaaag atgaagtctc aatcgaccaa aaaatagaga cagaaggacc agacttttta
    18541 gcagagatca cattgcaaga acctttagca attcagcatc ttttattaaa agaagcaatc
    18601 gaaaattgcg aaatccagtt ttcaaaaata gaagctaaaa tttttcatta ttatatgcaa
    18661 ggcgttgatt ttgtagacat ggctga
//
LOCUS       DAZUMB010000046        18548 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000046 DAZUMB010000000
VERSION     DAZUMB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.046"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..171)
                     /locus_tag="ACG80L_001409"
     CDS             complement(52..171)
                     /locus_tag="ACG80L_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5023648.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            350..2011
                     /locus_tag="ACG80L_001410"
     CDS             350..2011
                     /locus_tag="ACG80L_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5023649.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2081..2428)
                     /locus_tag="ACG80L_001411"
     CDS             complement(2081..2428)
                     /locus_tag="ACG80L_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS5023650.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2512..3453
                     /gene="opp3b"
                     /locus_tag="ACG80L_001412"
     CDS             2512..3453
                     /gene="opp3b"
                     /locus_tag="ACG80L_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5023651.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3454..4497
                     /locus_tag="ACG80L_001413"
     CDS             3454..4497
                     /locus_tag="ACG80L_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023652.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4503..5573
                     /locus_tag="ACG80L_001414"
     CDS             4503..5573
                     /locus_tag="ACG80L_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023653.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5563..6516
                     /locus_tag="ACG80L_001415"
     CDS             5563..6516
                     /locus_tag="ACG80L_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023654.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6528..7538
                     /locus_tag="ACG80L_001416"
     CDS             6528..7538
                     /locus_tag="ACG80L_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5023655.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7823..8644)
                     /locus_tag="ACG80L_001417"
     CDS             complement(7823..8644)
                     /locus_tag="ACG80L_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5023656.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8741..9019)
                     /locus_tag="ACG80L_001418"
     CDS             complement(8741..9019)
                     /locus_tag="ACG80L_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023657.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9287..11296
                     /gene="metG"
                     /locus_tag="ACG80L_001419"
     CDS             9287..11296
                     /gene="metG"
                     /locus_tag="ACG80L_001419"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS5023658.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11775..12362)
                     /locus_tag="ACG80L_001420"
     CDS             complement(11775..12362)
                     /locus_tag="ACG80L_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023659.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12537..13859
                     /locus_tag="ACG80L_001421"
     CDS             12537..13859
                     /locus_tag="ACG80L_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5023660.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14014..14790
                     /locus_tag="ACG80L_001422"
     CDS             14014..14790
                     /locus_tag="ACG80L_001422"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5023661.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14783..15352
                     /gene="rnmV"
                     /locus_tag="ACG80L_001423"
     CDS             14783..15352
                     /gene="rnmV"
                     /locus_tag="ACG80L_001423"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS5023662.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15345..16229
                     /gene="rsmA"
                     /locus_tag="ACG80L_001424"
     CDS             15345..16229
                     /gene="rsmA"
                     /locus_tag="ACG80L_001424"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS5023663.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16680..17309
                     /locus_tag="ACG80L_001425"
     CDS             16680..17309
                     /locus_tag="ACG80L_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023664.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17433..18542)
                     /locus_tag="ACG80L_001426"
     CDS             complement(17433..18542)
                     /locus_tag="ACG80L_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023665.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         6061 a         3340 c         3782 g         5365 t
ORIGIN      
        1 ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta ttcagataag
       61 ggactgtgac aagacttttg tcacagtttc tttttgttta ttctttttta tctttgtatg
      121 gcaataatag acaatataac gaaaattatt taatggcttt tggaagacca tggaaaacct
      181 tgtacaaagc tattttttct gaaaaattcc ttgaaacctc acgaaaaaat cattatttta
      241 tgaaaaagac aaaaaaaagt gttgattttt taataattat tcattaaact cttaaacaac
      301 ttcagaaaat attaattatt ctgaaaattc caataaaggg gtacgtaaaa tgaaaaagaa
      361 atttacattt ggtttggttg cggtatgcgg ccttgtactt gctggatgtt atggcggcag
      421 ttcagccaca agcagtagtt cagcaaaaag cggttcagca gatggaggag gcgtatttaa
      481 cttagtcgtg cctcaagaaa tgcctacagc tgacttatca gttgcaacag atacaatcag
      541 ttttacagca ttgaataacg tttatgaagg aatttaccgt cttgatgaag acagcaaacc
      601 acagccggca ggagctagcg agttggcaga agtcagtgaa gacggattaa cttacaaaat
      661 caaattacga gaagacgcaa aatggtcaaa tggtgatcct gttacagcag cggattacgt
      721 ttatggctgg caacgaacag tagacccagc tacagcttct gaatatgctt acctgtatgc
      781 gccagttgaa aatgcagaag acattacagc tggaaagaaa gataaatcag aactagggat
      841 caaagcagtt ggtgactatg aattagaaat caaattgacg aaacaaacac catatttcca
      901 atatctgctt gctttcccat catttttccc acaaaaacaa tccgtagtgg aagaaaatgg
      961 ggatgcctat gcatctgcaa gtgataaagc tgtttataat ggaccattca cattagaaga
     1021 ttttgatgga ccaggaacag atacagaatg gacttataag aaaaatgatc attactggga
     1081 caaagataca gtaaaacttt ctgaaatcaa agtcagtgta gtaaaagaat cttctactgc
     1141 attgaactta ttcaaagatg gccaagcaga tgacgtgatc ttgtctggtg agttagcaca
     1201 gcaaaacgca aacgatccag cttatacttc tgttaaagaa gcgcggacaa gctatatcga
     1261 attcaatcaa cgtgaaaaga attcaccatt caacaatgtg aacctaagaa aagctatttc
     1321 ttattctatc gatcgtgaag ctttagtaaa acaagtacta ggtgacggat cagttgtctc
     1381 tacaggattg atcccatcag atatggtaaa aagtccaaca acgaacgaag actttgcaaa
     1441 agaagctgga aaattagttt cttatgacaa agataaagca aaagaatatt gggaaaaagc
     1501 gaaaaaagaa ttgggcgttg attctttaga atttgatttg atggcttctg atgatgattc
     1561 atcgaaaaaa gtaatcgaat atgttcaaaa ctcaatccaa gaaaacttgg atggcgtaac
     1621 agtcaaacca acacctgttc cattctctgt acgtcttgac cgctcaactt cgggtgattt
     1681 tgacgcagta ctaggcggat gggctgctga ctatgcagac ccaagcagct ttactgattt
     1741 gtttgtcaca ggtaattcat acaaccgtgg tcgctggagc aatgcagaat atgacgaagc
     1801 agttaaagca gcttcatcaa cagacgctgg cgatgaacaa gcccgttggg atgatttcca
     1861 aaaagcaatc aagatcatct ctgatgatat gggtgtcgtt ccggtgtacc aaaaagcaga
     1921 aggtcaccta gtgaatgaaa aaatcaaagg tatcgttcac catgcggcag gcgcttcatg
     1981 ggattacaaa tggacatacg ttgaagaata atcgttaata ccgtgagcat gagaaaaaac
     2041 gaggctgcgg caaatgtcgc aacctcgttc tcatattcac ttatcctaac aaagaaaaaa
     2101 gcatgaaaat agcagaaaga gcaattagta ttccggcaat gatcagccaa aagctgtaca
     2161 taccgtagct ggcagacgag agcgaggaaa attctggttt tttctttcgg ttccttgttc
     2221 gtgcaagatg gaccgatcgc tgaaaatgat cgaagaaacc cgaagaaaag acccataaaa
     2281 aaccgccgat aatcagaaac ggcaatgacc ataagaatag atcattagat aattgaatga
     2341 gtgtaaagga atggttgatg agatttttta ttaaaataac cagaatcagt agaaaagcta
     2401 ctgcgtaggg gatagatttt tttttcattt gattgctcct ttatttttgg ttcattatct
     2461 aataatgtag cgaaacatct gtcagaagtc aaaagctagg gggaaacatt agtgaatagt
     2521 tatatcaaat atgtattaaa acgtgtcttt ttcatgatca tcacgctttg gttaattgca
     2581 acaatcacct ttttcttgat gcaattgctg ccgggcacgc catatacgaa tcaggaacgt
     2641 ttgagtcctg aaacaatcga gatgctaaat aaacaagtcg gtttggataa accagttatc
     2701 gtacaatatg ggatttatct ttctaatttg cttcaaggag attttggaat ttccttccaa
     2761 tttaaaaacc agccagtcgc acatttgtta gctggaagaa tcggaccatc cttgcagtta
     2821 gggttacagg caattatctt tggaacagtc ctagggacga ttttaggaac gatctctgcc
     2881 atgaagcaaa atacatgggc agatacatct tctacactcg tagcgatatt aggtcgttcc
     2941 attccaaact ttgtatttgc tgttttatta caatatatct ttgcaatcaa attacaagtt
     3001 ttgccaatcg caaaatggga tggctttgtg tacacaatct taccaacaat tgcgttggcc
     3061 atgtcgccgc ttgctgactc tgctcggttt atccgtacgg aaatggtgga agtactgcat
     3121 agtgattatg tggaattagc aaaagcaaaa ggattgagcc gttgggaaat cgcctttaaa
     3181 catggtcttc gaaatagttt gattccattg atgactttat taggaccgtt agctgtagca
     3241 ttgatgaccg gatcactggt ggtagaaaat atctttgcta ttcctggtat cggggaacaa
     3301 ttcgttaagt caatcacaac aaatgattat cctacgatca tggctgtaac gatcctttac
     3361 tcatttatgc tgatttttgt tattttgatc gtggatctat tgtacggtct agttgatcca
     3421 agaattcgtg tttcagaagg gagccgagga taaatggaaa tgataccaaa gcgttatgaa
     3481 acagttgcag atatccctgc ggatgaattt cttccattac aaaaaaatac cgaagaagaa
     3541 cgtgaacaga ttactgcacc ttctttgaat tttatccaag attcatggcg ccgtttaaag
     3601 aaaaacaaag ctgccgttat ttcaatggtt ttactattgg tcatcatttt gatctctgtg
     3661 atcacaatct ttgtttctcc tcatgatcca actgcccaaa acgtggctta tatcaatttg
     3721 cctcctcgta ttccgggaat caatatcgat ggattgaatg gaaaagcaat ggttggtggt
     3781 gaacttgttg ataaatatgc tcaagcaaac gtcccttcaa acgtaaattt ctttttagga
     3841 acagacggtt tgggacgtga tgtattaagc cgcttattta tgggaatgag aatctcgttg
     3901 ctgatcgcat ttattgctgc attactagat gtaacgattg gtgtagctta cgggttggtt
     3961 tctggtttgt taggcggacg tgtcgataac gtgatgcaac gttttttgga aatcttatca
     4021 ggaattccaa acctcgttgt catgattttg atgttggtcg tttttgaacc aggaattttt
     4081 tcaatcgtgg ctgctatggc catcacaaac tggataccaa tggcacgtat cgttcgtgca
     4141 caaacaatga aattaaaaga tcaagaatat gttttagctg gattgacatt aggtgaatca
     4201 agaatgaaaa ttgcattcaa acatattttg ccgaatatct ctagtgtcat tattattcaa
     4261 atgatgttta gtattccatc tgctattttc ttcgaagcct ttttaagctt tatcggttta
     4321 ggtcttacgc ctccgtcagc ttcattagga acgatgttga gcgaagggta caagacattc
     4381 ctgtatctgc cttatctatt atggattccg gcagtaactt tatcggttat catgattggc
     4441 tttaacttac ttgctgatgg attgcgtgat gcctttgatc ctaaaatgaa agagtgaaga
     4501 cgatgacgaa aattttagaa gtgaaagact tagaaatctc ttttgatacg tatgctggaa
     4561 aggtacgagc gattcgcggt gtcaattttc atttgaataa aggagaaaca ttggcgatcg
     4621 ttggggaatc tggaagtgga aaatcagtga cgaccagaag catcatgcgc ttgctgtcaa
     4681 gcaacgccaa tatagatgct ggtcaaatcc tctttaaagg taacgatatc gttggaaaat
     4741 cagaaagaga gatgcaaaaa atccgtggta aagaaatagc gatgattttc caagatccaa
     4801 tgacctcttt agatccgacg atgcccattg ggaagcaagt cgctgagtcc cttagaaagc
     4861 ataataaagt ttcaaaaaaa gagggacaaa aagctgcatt agaattgctt aaattagttg
     4921 gaatacccga agctgagaaa cgtatcaaca gttatccgca tcagttctca ggaggtcagc
     4981 gtcaacggat cgtgatcgcg attgcactgg tatgttatcc ggaaatcctg attgctgatg
     5041 aacctacaac agcgcttgat gtgactattc aagcacagat tttggagctg ctgaaagaaa
     5101 ttcagacaaa aatcgataca tcaattatct ttatcactca tgaccttggt gtagtagcga
     5161 atgtcgctga tcgtgtcgct gtcatgtatg gaggaagaat cgttgaagtt ggaacatcgg
     5221 aagaaatttt ctacaacccg caacatccct atacttgggg actgcttggt tcaatgccga
     5281 cattagatag tgcgaacgat cgattgtatg ctattcctgg ttctccaccg gatttattga
     5341 acccgccaaa aggagacgca ttctatccaa gaaatgaatt cgcgatgaaa atcgatgcag
     5401 aacaagaacc accatttttt gaactatcaa aaacacacaa ggcagcaact tggctacttg
     5461 ctccgcaggc acccaaagtg acaccgccag aagaaatcca acgtcgctgg gctatcttta
     5521 aagagaaaca aaaacatact tacggtcaag gaggaattgc tgatgccaaa taaactactt
     5581 gaagttaaag gattgaaaca gtatttcaat gtaggaaaaa agaatgaagt acatgctgta
     5641 aatgacatca gctttcacat ttatgaagga gaaacattcg gtttagttgg agaatctgga
     5701 agcggaaaat ccactactgg ccgtacgatc attcgcttga atgaacctac tgatggtgag
     5761 atattattcg acggtcaaga tataacgaag atcaaagata aaaaaggttt gacgaaattc
     5821 cggcatgatg ttcagatgat ctttcaagat ccatacgctt cgttgaatcc tcgtatgaaa
     5881 gtcagagata tcattgccga aggaattgat gttaatggac tagctaaatc atctgaagag
     5941 cgtgcaaaaa aagtcgatga tctattgaga actgttggat tgaatccaag ccacggtact
     6001 cgttatcctc atgaattttc aggtgggcaa cgccagcgta ttggtattgc acgagcattg
     6061 gcagtaaaac cgcgttttat catttgtgat gaaccaattt ctgcactaga tgtgtcgatc
     6121 caagcccaag tggtcaattt gttgcaagat ctccaaaaag aacatcaatt gacctatcta
     6181 tttattgcac atgatttgtc gatggttaaa catattagtg accgaatcgg tgtaatgcat
     6241 aacggcttgt tattagagat gggaacaagt gatgaaatct ataatcacgg tgttcatcct
     6301 tatacagaaa gtttgttatc tgctattcct ctaccagatc cagaccatga gagaaaacgc
     6361 agaagaatca aatatcaacc tgaaccagat gacggacaag tacgtcaact tcgtgaaatc
     6421 gcaccagaac attttgtcta tgcaactgag caggaagtcc catattacgc aaaaaaacta
     6481 aaacgacaaa aggaatctct tctggttgct aactaggagg gacatagatg ctaaaagctt
     6541 ttaaattccg tatgtatccg acagaagaac aaaaacagca attgattcgt acttttggat
     6601 gtgctcgatt tacctataac catttgctga aaaagcggca gaaaagttgg caacaaacag
     6661 gagtagccaa tttttcgctc acacccgcta cattaaaaaa agaataccca ttcttaaaag
     6721 aagtagacag tcttgcttta gcaaatgcac agcttaattt ggatcgggct tttcgcaatt
     6781 atttcaaggg acgtgccagc ttcccaaagc ttaaaacgaa aaaaagtatg tggcaatctt
     6841 atacgaccaa caaccaaacc cgtacgatct atctggaaaa tggctactta aagttaccaa
     6901 aacaaaaaga actaatcaag ataaatcagc atcggccagt tgaaggatcg attcgctccg
     6961 ctacgatttc tgctcgatat aatgaagaat tttatgtcgc actactttgt gatgtttcgc
     7021 cagtaaaaaa agaatcttta gctaaatgga tcgggatcgc ctatcatccg aagacattga
     7081 tccaaacatc tcgacctcta gaagtaactt taccaaaatt tcaccagaca gaagaaaagc
     7141 tgcaacatgc acagcggaaa ttgaatgtga aagtccgctc cgctcaccat agaaagatca
     7201 ggttagatca ggcaagcaat tatcagaaac aaaagagaaa agtaatggat ctttatctga
     7261 aacaaaagaa ccagcgagaa gattatcttg aacaattatc tgggaaattg gtcaaacaat
     7321 atgactactt atttgtagaa tcttttccaa aagaagaggc acatgctgat ttttctattc
     7381 acgactggca taaattaata acaaaattgc gttataagtc ccaatggtat aataaaaaat
     7441 ttcttttgat caataccgac ggagcagaag aaagcaattc cgtaagaaag agccaagtag
     7501 tagaaaaaat gggcagacat tcggttatca agggataaaa aatgaatgaa actgatagga
     7561 gcgttagggt tagccttgtt aataaaaagt cttaatgact tttgtttcta ggaatttttc
     7621 ttttgaagaa aagctcacta attatgacaa accaataaaa caatagcaga aaaagttcgt
     7681 acgagctttt tctgctattg ttaatttttt attcagaaag tttacgaata catcaaaatt
     7741 ctgaaacaaa aaacgtaact cacaaagagt tgtttttgcg ttttttcttg aatgattagg
     7801 tctgaatctt ttgctcacaa ccttattcat ggtgccccgg cttgagctga tagatttttg
     7861 aaggacggcc gattcctaca ggacgttcac cgatctcatc gaaatattgg agcatagctt
     7921 tcttaaaatt ggaatgatcg atcgttttat aatcgatccc taagaattta gcaaatactt
     7981 ttcgagcttc cgtaatcgtg aaatctttac ctaaaacttg tagaacttgc ggctcgtgtt
     8041 ccattttatt gactacacgg ttgaatgctt tcaagatgat ttgactatgg tcaaaagcca
     8101 aagtatcttt gccagaagac tctccagtga ctaaatcaag cgagatagcc acttctccgc
     8161 tagataaatg gatcttgtta ctatgacgtt ctagtgtgaa ccagcgtact tccttggcat
     8221 catctccggc gattagcggt tcttctccaa taaaggctaa gtagctgact gtgacgaccc
     8281 agcctcgcgg atctcgatta ggcgtactga atgtatgcag ttgctcaata ttttcttttg
     8341 aaataaccac accagtttct tcctttgttt ctcttaagac gctttctcca gttgactcat
     8401 ccttttggac aaagccacca ggcaatgccc atgaatttcg ataaggatgc ccttttcttt
     8461 ggatcaacaa aactttcagc tgatcttctt ctttgttata gcacatcagt acgatatcga
     8521 cagtcaaaga gggcttgtca tattctggac gttcctgttt ttgataccag ttaagaaatt
     8581 cttcttcact tgctttatgt tcataatata atttttcttc ttctttagaa gcaaaacggt
     8641 tcatctgacc acctccattg acagtaaaag tactttttac cttattgtag cagactctga
     8701 aaaaaaacca aggatttact tgaaaaatct aggttatcat ttaaatatga aaaataaatg
     8761 gaagtatgag attgaataaa aagaaaagca gataaaacgg caatacttta taccgctctt
     8821 cacgaatggc aaagaagcca caaataacga tatatacgtc aatgataaaa cgcaaaatcc
     8881 aagctgctgt cataaaactg ttcaaatgcc catggaggtt aaaataacca tacatacgaa
     8941 tataaaaata gctgaatatt aaatgtgcta atgaaagaaa agcaatacta agtgtaaaaa
     9001 aaagatgttt cttctgcatg attgttccag tcccttacta agatagatag tctttattat
     9061 accacgtgtg aggggagaaa taaaaagccg ttttttagta gatatgaaac aaaacaaaaa
     9121 cccgcattta ttcaacctat tcatcctttt aaaggataga ttgaacgtag atagaagtcg
     9181 tttatccagt ctttttcatt ttgaaaaaaa gctaacagca tgcttttttt atggtatact
     9241 agcaaggaaa cgaattcgca tcatgcgata aaggaggaag agccccgtgg cagaaaagga
     9301 aactttttat atcactacgc caatctatta tccaagcggc aaacttcata tcggcaactc
     9361 gtatactacg attgcatgtg acgcaattgc tcgttataaa cggttgatgg gctttgatgt
     9421 gttttatctc actggagtgg atgaacatgg tcaaaaaatc gaaacaaaag cagaagaact
     9481 aggtgtaaaa ccgcaagaat atgtagataa aatggcggct gatgtcaaga aattatggaa
     9541 aacgcttgat atcagttatg ataaattcat tcgcacgaca gatgattacc ataaagcagc
     9601 tgttcaaaaa atatttgatc gtttattaga acagggagat atttacttag gtgaatatga
     9661 aggctggtac tctgtttctg acgaagaatt ctttacagaa acacaacttg cagaagtgta
     9721 caaagatgac gaaggtaacg tgatcggtgg taaagcgcca agtggacacg aagtagaatt
     9781 ggtaaaagaa gaatcatact tcttccgcat gagcaaatac gcagaccgtc tattggaata
     9841 ctatgaagaa catcccgaat tcatccagcc agaatctcgt aaaaacgaaa tgatcaacaa
     9901 ctttatcaaa ccaggattag aggatcttgc agtttcacga acaactttta cttggggagt
     9961 accagtcaag agtgatccga aacatgtggt gtatgtatgg atcgatgcat tatccaatta
    10021 tattactgca ctaggatatg gctccgaaga tgaaagtcta ttcgaaaaat actggcctgc
    10081 agatgttcag atggtcggta aagaaatcgt tcgctttcat acgatctatt ggccaatcat
    10141 gctgatggcc cttgatttgc ctctacctaa aaaagtattt gggcatggct ggctattgat
    10201 gaaagacgga aaaatgtcta aatcaaaagg aaatgtcgtt tatcctgaaa tgcttgtgga
    10261 acgttatggt ttagatgcac tccgttatta tttgcttcgt gctgttccgt ttgggtcaga
    10321 tggtgtcttt acgccagaag attttgtttc ccgtgtcaat tatgatctgg caaatgatct
    10381 agggaactta ttgaatcgaa cgattgctat gatcaacaaa tattgtgatg gactcgttcc
    10441 tgactatgcc tcacttgtca ctccatttga tagtgaactg tctacgacgg ctgctaacgt
    10501 tgtcggacgt taccatgatg caatggaaaa aatggaattc aatacagcat tggctgaaat
    10561 ctgggtgctg atttctcgtg caaacaaata cattgatgaa acagaaccat gggtattagc
    10621 aaaagacgaa gaaaagaaag ctgaattgaa tagtgtcatg atccatttag ctgaatcttt
    10681 gcgtatcgca gcgatattac ttcagccaat catgacagaa acgccagaga agatcttcaa
    10741 tcaactagga ttggactctg aaacaatgaa tcttgaagga ttacactttg gcgaatttcc
    10801 ttcaggaaca aaagtagttg caaaaggtac accaatcttc ccacgtttag atatggaaga
    10861 agaagttgct tttattcaag aaaaaatgtc tgaaggtact caaacaaacg aagatacagt
    10921 caaatgggat ccagaagaaa cagaacttgt ttcaacgaaa gaaaaacaaa tcaaatttga
    10981 tgtatttgaa aaagtagaac taaaagtagc agaagtcatc aactgtcaaa aagtagaagg
    11041 ggcagataag ttgctgcaat tccgactaga tgctggagac agccaagacc gtcaaatctt
    11101 gtcaggaatc gctgaatttt atcctgatcc aagcgaattg atcgggaaaa aagtcgtcat
    11161 tgtagcaaat ctgaaaccac gaaagatgcg tggacaaatc agtcaaggaa tgatcctttc
    11221 tgcagaagca ccagatggct ctttacaagt aatcgaagca ccaaaatcca tgccaaatgg
    11281 ctcagaaatc gcatagaaat tcaaaggggg tatgacacta gttgtgtcat accccctttt
    11341 tccgaaattt gagaagcaat tttcgtaaat tccttcttat tacttgaagc caatcatata
    11401 atacagaaac agagaactat ccaaaataaa tcctagaaaa agaagtacga gcttcgctga
    11461 tttttgttaa tttagaataa tactaatagg aatcgtaaaa acagaaaaaa agcataatga
    11521 taaagggtcg ggcagatttt aattgattca tctggtactc ttttttattg tccttacatt
    11581 cataaagttt gaatgcaatg actttattac atctatattt atatctaaca gtagcgttga
    11641 tgtgttaatt ataagttcta tcactttatg ttcaattagt ttgtgctctt atatgtgtga
    11701 ttgaaaatat agataaaaaa acaatcatcg gctttgactt cgatgattgt tttctttatt
    11761 ttgtaaaaat cctatcactt gattttatgg taacctttta tttttgtctt caaacggctt
    11821 ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga cataccagaa aatcgtacgt
    11881 ccgataccat aatgttgata gagctttgtc aacatcacgt gtaacaagat caaccctaaa
    11941 agcaaggagc cagctataaa ccaatgatta tcccaatgca tgacttcact aagtgccatc
    12001 actccaaaag gatagaaata aaaatagatc ccgactaagg tcatccgcca gttctttgta
    12061 aatgttccga tattcaataa aaagaaaatc gctgaaagca aaataccgat aggcggaaaa
    12121 agcccaacca aaaaggaata caacatcccc cacaagatca tcacaggacc tttgatcaat
    12181 gcaacgattg cgatgaacaa agtcagcaat attagttttt gtgataacgt tgcaacagtc
    12241 attaatccaa ataaaattaa tgtgaaccaa tgccaatggg actgctgttg ttctttacgg
    12301 gaaaaatcga cccggttgga taaataatct gctaatttcg gataagaacg cttaaaatcc
    12361 ataaactgtt cctccttcgt ggaaaaggta ggtttagtat acgaaaaata aaaaagaaat
    12421 acatccgtct taagagggag tttttcactg aaagtggaga aaaaagaatg gtaaattaaa
    12481 atattctcag ttatactgaa aattatacca aatttcaaag aagaaagagg atgggaatga
    12541 aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt agctaccgtt atgggaaccg
    12601 tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac tgctagtact cagtctacaa
    12661 gtttgacatt gctagcttgg cttttgggtg gtttcttaac gatctgtgct ggattgacag
    12721 tggctgaatt agctacagca atcccggaaa ctggtggagc tgtaaaatat attgaagctg
    12781 cgtatggaaa attgcccagc tttttattag gatgggcgca aagcttgatt tactttcctg
    12841 ctaatattgc tgcattatcg attatctttg cgacacaatt gacgaactta ttacaattat
    12901 ctaccgatta tcttttgttg attgcagtga tcaccgctgt ttctgtgaca gggttgaatt
    12961 tgttagggac aaaagtcggt gcttctgtac aatcagtgac gttgattgtt aaattaatac
    13021 caattgctgt gattgtcata tggggattat tgaccccagg acaaggaact gtccagttat
    13081 ttccaatcga agctggaaaa gatgttactt ttgtcgaagg attgagcagt gcgttgttag
    13141 caacgttgtt tgcttatgac ggctggttag gtgtcggtgc aatggcaggg gaaatgaaac
    13201 ggccggaaaa agatttgcct aaagcaatta ttcttggttt gagttttgta acagtcgttt
    13261 atctattgat caattttgtt tttttgaaaa ctttgccgat tgatcatctt gccggtaatt
    13321 tgaatgcagc ttctgaagca tcagacgtca tttttggagg aatcggtgga aagctggtca
    13381 ctattgggat cttgatctcg gtatacggtg cattgaacgg ttatacacta actgggatcc
    13441 gcgtgcctta tgcgatggct ttagaagacg agctaccttt cagtaaacag ctgacaaagc
    13501 tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt ccagttagca gtagcttgta
    13561 ttatgatgag ccttggttct ttcgactttt taacagacat gttgattttt gtgatgtggc
    13621 tattcagttt actgattttt atcggtgtct ttattttaag aaaaaaagcg ccggaattgc
    13681 cacgtccata taaagtacca ctttatccaa tcgttccgat tattgcgatt ctcggtgcta
    13741 ttttcatttt aggtatgacg atgatgacgc aaacgaaatt agcattgatc ggtataggtg
    13801 ttaccttgat aggtatccca gtctattatc aaaaaaagaa aaaacgctca gaagagtaaa
    13861 atatttcaac aacagacaga aaaagtacga actaggtcct ctagatcttc gtgctttttc
    13921 tatttcaata aaacaagttc ttctattttg atttttgcta cttctgcaag tagtgcttat
    13981 gtgctaaagt aagaatgaaa aggagagata aacatgatat ttgattctca tacccattta
    14041 aatgctgaac agttcaatga agatattcca gaaacgattc agcgagcaca agaaatgggg
    14101 gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga ttgaaaagtc tttgcaatta
    14161 agccatgact atccaaatat atacagtatt atcggctggc atccgacaga ggctggcagt
    14221 tatacgaaag aaatcgaaaa taaattacaa gaacaattga ctttaccaaa ggttgtagca
    14281 ttaggggaaa ttggcttgga ttatcattgg atggaagacc cgaaagaagt ccaagataaa
    14341 gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc taccaatcag tattcatact
    14401 agagaagcac ttgaagacac gtatcaaatc ctaaaagaag aagatgttcg agatatcggt
    14461 ggaatcatgc atagtttcag tggtgattat gaatgggcaa aacgatttct tgacttaggg
    14521 atgcatattt catttagcgg tgtcgtcact ttcaaaaaag cattagacgt ccaagaggct
    14581 gcagcaaatg taccaatcga acgattactt gtggaaacag atgcccctta tctggcacca
    14641 gttcctttta gaggaaagcg aaatgaacca ggctatactc gttatacagt agaaaagatt
    14701 gctgaacttc ggaatcttcc gacagaagaa gtggctgccc aaacatggac aaatgcgcac
    14761 cgattatttg gtctaagcga acatggataa gatgaagatc caagagatta tagtcgttga
    14821 aggaaaagac gatacaagaa ggctgcggga agtagttgat gcagatacga tagaaacaat
    14881 agggtctgca atcaatgaag agatcctgat gcaaattgaa catgcccaag agactcgtgg
    14941 agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa ataagaaaaa tcattatgga
    15001 agctgttcct caggcaaaac atgctttttt gccgcgcaca caagctaaac caaagaaaaa
    15061 gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt ttagaagcat tgaaaaaggt
    15121 cgtgacgcca gacatttccc aaacggctgt ttctgaaatc actagacaag accttttaga
    15181 ctatggttta atagctgggg cacaagcaaa agaaaaacga gaaaaactag gtgacgagtt
    15241 gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt ttgaagatgt ttcgtattac
    15301 gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa caacatgagt gaatatcgtg
    15361 aaattgcgac accctctaga acaaaagaga ttttattgaa acatggtttt tcatttaaaa
    15421 aaagtttagg acaaaatttt ctgactgaac caaatatttt aaggaaaatc gtagaaactg
    15481 cgcaaataga tgaacatacg aacgtgatcg aagttggacc aggaatcggt gcattgacgg
    15541 aacaattagc caagcatgca aaacaagtcg tagcatttga gatcgatgat cgtttgatcc
    15601 ctgtattggc tgatacgatg caaccctatg acaatgtgac tattgtacat caagatattt
    15661 taaagaccga tttgtcaaca gctgttagag aaacatttca tgaggagctt ccgttaaaag
    15721 tcgtagcgaa tttaccttac tatattacga caccgatcat gatgcatttt cttgaatcag
    15781 atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga agtagctgat cgtatctctg
    15841 ctgaaccagg aacgaaagct tatggttcat tatcgattgc tgttcaatat tatatggaag
    15901 ccagtttagc attcatcgta ccaaaaacag tatttgtccc tcagccaaat gtagattcag
    15961 cgattttgaa actgacaaga agagatatac cggcagtcga agtgacagac gaaaaagcat
    16021 ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa aacgctttgg aataacttgc
    16081 agcattcgta tggcaaagat gaccagacaa aagcatggtt agcaaaaagc ttggaaacag
    16141 ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt acaagaattc gccgcattaa
    16201 gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt gtgacagaag ttgtgaaaaa
    16261 gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat gtttttcgtt
    16321 tgtttgggct tgttaccgca gatgcaagct tttgaacacc gtttaagaaa gaggttgtgg
    16381 gcctgccgtt taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt
    16441 gagttagttg gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag
    16501 atgtgacaca aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac
    16561 ttgatactca cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta
    16621 gtaagtgagt tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa
    16681 tgagaaaaaa ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca
    16741 ttatcccaat ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc
    16801 atgtgccatt atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag
    16861 gtacgacatt tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac
    16921 atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa
    16981 aaaatggaca gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg
    17041 tgattcattc tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc
    17101 ctgaaacata ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg
    17161 ggcttatcca cagtttagtt gattataata ttgcttattt tatagcagga acactcagca
    17221 aatattttga tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc
    17281 aagggtacgc gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct
    17341 agtaatggat aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa
    17401 catcttctgg cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt
    17461 tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt
    17521 ttatggaaat ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa
    17581 agcatcgttt cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca
    17641 ggtgaagctt caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata
    17701 acttcttttc cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga
    17761 agcttaagcc catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc
    17821 atggcaggag atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct
    17881 ttcggtgtac cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata
    17941 gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga
    18001 agtttggcaa tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc
    18061 tcatccatta agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg
    18121 cgttgtccac cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc
    18181 tctgctgcat tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag
    18241 ccaaaagcca tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc
    18301 atggcaatat cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt
    18361 tctccttcag agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa
    18421 ccggaaggac caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt
    18481 acagaataat tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc
    18541 ataatacg
//
LOCUS       DAZUMB010000047        17749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000047 DAZUMB010000000
VERSION     DAZUMB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.047"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1273)
                     /locus_tag="ACG80L_001427"
     CDS             complement(<1..1273)
                     /locus_tag="ACG80L_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5023666.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIIS"
     gene            complement(1571..2506)
                     /gene="murB"
                     /locus_tag="ACG80L_001428"
     CDS             complement(1571..2506)
                     /gene="murB"
                     /locus_tag="ACG80L_001428"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS5023667.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2579..3499)
                     /locus_tag="ACG80L_001429"
     CDS             complement(2579..3499)
                     /locus_tag="ACG80L_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5023668.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3612..4364
                     /locus_tag="ACG80L_001430"
     CDS             3612..4364
                     /locus_tag="ACG80L_001430"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS5023669.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5228..5761
                     /locus_tag="ACG80L_001431"
     CDS             5228..5761
                     /locus_tag="ACG80L_001431"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS5023670.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6251..6775)
                     /locus_tag="ACG80L_001432"
     CDS             complement(6251..6775)
                     /locus_tag="ACG80L_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS5023671.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(6768..7241)
                     /gene="tsaE"
                     /locus_tag="ACG80L_001433"
     CDS             complement(6768..7241)
                     /gene="tsaE"
                     /locus_tag="ACG80L_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS5023672.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7364..8344)
                     /gene="pta"
                     /locus_tag="ACG80L_001434"
     CDS             complement(7364..8344)
                     /gene="pta"
                     /locus_tag="ACG80L_001434"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS5023673.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8497..9177)
                     /locus_tag="ACG80L_001435"
     CDS             complement(8497..9177)
                     /locus_tag="ACG80L_001435"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5023674.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9303..10172
                     /locus_tag="ACG80L_001436"
     CDS             9303..10172
                     /locus_tag="ACG80L_001436"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5023675.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10198..10713
                     /locus_tag="ACG80L_001437"
     CDS             10198..10713
                     /locus_tag="ACG80L_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS5023676.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(10760..11323)
                     /locus_tag="ACG80L_001438"
     CDS             complement(10760..11323)
                     /locus_tag="ACG80L_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5023677.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11378..11557)
                     /locus_tag="ACG80L_001439"
     CDS             complement(11378..11557)
                     /locus_tag="ACG80L_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023678.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11634..12143)
                     /locus_tag="ACG80L_001440"
     CDS             complement(11634..12143)
                     /locus_tag="ACG80L_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023679.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12239..12619)
                     /locus_tag="ACG80L_001441"
     CDS             complement(12239..12619)
                     /locus_tag="ACG80L_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5023680.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            12949..13698
                     /gene="pnuC"
                     /locus_tag="ACG80L_001442"
     CDS             12949..13698
                     /gene="pnuC"
                     /locus_tag="ACG80L_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS5023681.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(13882..15159)
                     /locus_tag="ACG80L_001443"
     CDS             complement(13882..15159)
                     /locus_tag="ACG80L_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023682.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15238..15882)
                     /gene="pcp"
                     /locus_tag="ACG80L_001444"
     CDS             complement(15238..15882)
                     /gene="pcp"
                     /locus_tag="ACG80L_001444"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS5023683.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16081..17025)
                     /locus_tag="ACG80L_001445"
     CDS             complement(16081..17025)
                     /locus_tag="ACG80L_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS5023684.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17027..17713)
                     /locus_tag="ACG80L_001446"
     CDS             complement(17027..17713)
                     /locus_tag="ACG80L_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS5023685.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         5150 a         3640 c         3085 g         5874 t
ORIGIN      
        1 gagaaataat gtaatcggta taagcagatc cttgcgaaaa atcaatacag acatgaatcg
       61 gtgcttcaaa atatgatacc ggacaaatac cgaccaataa aaagagataa tcataaaata
      121 agcgaatcat gaaacgttca tcttttccca agaaatagcg tttattctct tgtatgacct
      181 tttggattgc tgtactgaat attggatacg tctcgaagaa aaactgtagc tgctttttag
      241 acgtaaagct agtcatctca aagtcgaata tccaattttt tcgattgaca tgcatcatac
      301 ccatacgaag ttcttttcgt atatatagtc tttcttcttc agagatttca atcgaaaatg
      361 gtaattgatc aatcaattcc actgacattt tttgactaat catgtggata tcatgaaaat
      421 gagcttccgc aagtccaaat gcgccatcta attcaaattc ttcttccgtg atcaaaaaca
      481 acatgatata agaaatctca tcaatgatct gctctttagg gatatgaaga aaagaaatat
      541 gtgataaatc cttgatgatc ttcattcctt ctgagctctt aaagtattca tctgaaaaga
      601 aacgttttcc gattggttga ttcccatagg ccctcaacaa actaatagtg gttagcaaat
      661 tgaatttgat tcgttttgtt gcagacagtt ttaaattgaa cagatattca aaatattgca
      721 cgatactctt tgcttcattc atgacgcttt ctctaaatgg catagaaaaa ccattaaaga
      781 ccccgaaata taccccaaat aatacgttcc gtatgtttat ttcttcccca actaatatgt
      841 ttttctttaa ctttatttga tgctgttcaa gaaaagagac taattctctt ctttttacat
      901 aggctcttga tatgcttaaa aaatgattct ctgcaaattt ttcgatcgtt acttttgaat
      961 ccacgatctc cgttaacagt cgagcaattt ttgatcgttg aaagtagatt cttcttaaat
     1021 ttttgatatc cgtcaaattc agacgttcga catggatctc tgcttcttct gaaatttgga
     1081 tctctggctt tgttttgaat tcagtaaatt ctttatttaa ttctatgata taatttcttg
     1141 ttttaaattt agacaaacct aatgtttcat ataacttagg cagcactaca taattctttt
     1201 tctcaaattc taaatatcta gctagcagca gtttttctct atcttcctct tctataaggt
     1261 aatccaactt catatcctac acctcaaaaa atgcctcata ccaattactt gatatgcaga
     1321 tgaaagttca tcttttcacc tatactgatt ttatgatgga ttatgcttgt tttttcatca
     1381 gttattctca ttttttatct tttttttgag aatattgcaa aaaatggaaa aatttatgag
     1441 gaaatattct atattttttg ttttattgtg ttaaaatgta caagatttgc gccatttgcg
     1501 taaacaacca caaaaaagag caggacggcc accgtcctgc tcttttactt attttaagtt
     1561 ccattactta ttaattggct aaacttctgc ttgtttctga tattgcttgt ttttctccaa
     1621 tgatccgcac ttctgtttct aaagacacat caaatttttc tttgattact tcttggatgt
     1681 gcgcaatcaa ttcaatataa tctgtcgctg tcgcatgatc gatattgacg atgaatccag
     1741 catgtttttc agagacttgt gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga
     1801 tcagctgacc tgtatagtgt cctactgggc gcttaaagac acttccgcat gaagggtatt
     1861 ctaatggctg ttttgattga cgcaattcgg tcaactcatc cattcttgct ttgattgctt
     1921 cataatcccc tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt aattcttgga
     1981 ctttactgtg acgataagaa aataccattt cgtcttttgt caaagttttc aatgttccat
     2041 cttcaagcaa aagatccact tcagcaaaaa catctttgat ctctccgcca tacgcacctg
     2101 cattcatata aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag
     2161 tcaatgattc tgccaaagct gcataagtcg tatcaatcag tgaggcacct gcttccgcaa
     2221 tgaccatcgt tcctttgaca tgaatctgtt tcatttcagt cagcatgatg actacaccgc
     2281 gaatcccgcc atcttgaacg atcagattgc ttgcatttcc taataccatc caaggtatat
     2341 catggattcg gcaataatcg atgatttgct ttacttcttc ttgttttttc gggaatgcaa
     2401 ggacatcagc tggtccacct gttttcgtaa acgtatagtt catcaatggt tcatcaaaca
     2461 atatagtaat cgtggctaag ctttcaacta tttcttcttt attcactgag gattttcctt
     2521 tcttttctca aacgtcctca ttattctatc acgcttaata caatcttgcc agtcttgttc
     2581 aaagttgatg acggtagtga tctttaagca cttcctcaca taattcatga cttcttacaa
     2641 cttctttttg tttaagcaat tctgtttccg atttacctaa acggatctga gaaaagaagg
     2701 cctctaccat tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt
     2761 tcactttttt tccatcagcc tcttgaatga caagctgggt cagatcgttt acttcataag
     2821 taccattttt acttgttact tgatattttt ctatattagc gccacttgct aaatccatcg
     2881 ttaaaatagc cgtttgattt tgtgtttcta attgtaaaat ggcatattcc aaaaattcct
     2941 ttgtttctct tatctggtat tttgttctca aaatgggttc atctaataaa tacacagctg
     3001 tatcaataag atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct tttgccgcta
     3061 ttcgattttt ttgcaggtgg atcaaccgct tatctgggat acgtttcaat tcttctacca
     3121 taggagcaaa gcgccggttg aaaccgatca tcaaaattat ttggttctta tcagctaatt
     3181 cttgcaattc acgaacttca tttagatgga cactcagtgg tttgtcaata taaacatgga
     3241 taccattttc tagacatttt ttggctaatt cataatgtac ttttgttgct gcgtggatca
     3301 tacatgcttc gattttttct tctaaaagct catcgatcgt ttcatataga tgttggaacc
     3361 cgtactcttt ttgaattctt tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa
     3421 gtgttccttg ttttttcgca taagttggta aatatgcttt ttctgcgata ttccctacac
     3481 cgattacacc aatcttcatc tactttcctc ctggatcatt tagttacttt catcttacac
     3541 cattttttac taaaatgagg tacaatagta tcgcaaaggt aaggaaatta attcgaagga
     3601 gtgtgctttt attgaaattt atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata
     3661 aaaatttttt agaagttttt catgagttag acgccgattt tttctgtctg caggaaacga
     3721 aactccaagc aggacaaatt gatttagatc ttccaggtta ttatcaatat tggaattacg
     3781 cggaacgaaa aggctattcc ggcacagcga tttttgctaa aaaaccagca ttgaatgcca
     3841 cttacggaat gggtatcgat atccatgata cagaaggccg tctgattact ttggaatatt
     3901 ccgatttctt tcttgtaacc tgttacacgc caaattctca aagcgaatta aaacgtttag
     3961 attaccgatt agaatgggaa gaagcctttt ataattattt ggaaaatcta aaaaaacaaa
     4021 aaccagtcat cgtgtgtggg gatctaaatg tcgctcatca aaagattgat ttgaaaaact
     4081 ggaaaacgaa tcaaaaaaat gctggattca ctccagaaga aagagcggct ctttctcgac
     4141 tgttggataa cgggtttatc gatacattcc gttattttta tccaacccag gaaggtgtct
     4201 actcttggtg gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt
     4261 actttttgac gagcaaagat ttagagccag gactagctga tgcaaagatc catacggaca
     4321 ttatgggcag tgatcattgt ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag
     4381 tcgtttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca ttttgcataa
     4441 atttggcata ataccgagaa actggctttt gaacactgtt tatcgagaga ttgtgaaaaa
     4501 gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt
     4561 tggttgggct tattaccgca gatacaagct tttgaacacc gtttattaag aggttgtgaa
     4621 aaagctgcac tgcatcaagt aataagaaca gccaaacaaa aatagttcct catatttttc
     4681 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt
     4741 gaaaaagctg cactgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt
     4801 ttcgtttggt tggacttatt accgcagatg caagcttttg aacaccgttt attgagaagt
     4861 tgtgaaatca gcgtcctgac ctgagtatta gagaaacaaa tggcaaaata gttcctcata
     4921 ttttgctatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagaa
     4981 gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac
     5041 aactttgcat aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca
     5101 caaggaatga gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt
     5161 ttttgttaaa tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt
     5221 tacagaaatg aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc
     5281 ttgttctctt gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt
     5341 gatacagcct gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga
     5401 agacgtacgc aatgcgccga aattcccaga agtctggcaa actatccaac catattttca
     5461 aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg
     5521 cttagattat tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag
     5581 ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa
     5641 catccaatta gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct
     5701 gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta
     5761 aaagaatact tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca
     5821 aggagcaatt tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg
     5881 agctggcagg agaacaccgt ttattggaga ggttgtgaaa aagctgttct gcatcaagta
     5941 ataagaacag ccaaacaaaa atagcttctc atatttttca tttggttgga cttattaccg
     6001 cagatgcaag cttttggaca ccgtttacca agaggttgtg aaatcagcgt tttgacctga
     6061 gtattagaga aacaaacggc aaaatagttc ctcatatttt gccgtttttt gagctaatac
     6121 cgaaggtcag ctgattgaac accgtttatt caaaaaaaat ggactgcgac acgacttttg
     6181 tcacagtccc ttcgtgtgtt tttctgcctt ctcaaaagga tgacagtttc tcactctttt
     6241 cctatttctt ctattcaatc aaataactca ttttatatac atctaaaaat tcacctagat
     6301 ctgttcttgc ccctctaggc atgaccgctt cgatttggaa gcccattttt cggtaaaggt
     6361 gtacggctct ttcattacga acttgaacgt caagctccaa tcgaaaaagt acacccattt
     6421 ccttcgccca actgatgatt tcttctagca taagagtgcc tagtcccatt ccccaatatt
     6481 cctgcagaat actgataccg acttctccca cgtgagacaa acgaaattga ctgtctgcct
     6541 tgacagatgc tgttccaata atcgtccctt catagattgc aagcagcaat aaattattat
     6601 tgctttcgta tatataatcc aactcttctt ctaaggtttc tggtggtaac aggagttctg
     6661 cttcatccaa aactaaaaat tctgtctcct tattaacttg tcccatcatc aaaagaatgt
     6721 cttttgcatc tcctggcact gcttcccgca agatgatccc aacttcttca ctcatgattt
     6781 tcagcccatt tttctagaat tcgctgtttg aaggtttcac tttgctcccc gtatgagatt
     6841 agtttcacat accgcttttc taaatctgta tcactttttt ctaaaatcag ttccaaataa
     6901 tcctccggca gtgcttcttc taatagattt ccccattcaa caacggaaag cccatcacct
     6961 tcaaaatatt catctaaccc taaatctgct ccgctagctg ctacccgata aatatccata
     7021 tgatacaacg gaatccgacc ttgactatat tctctaataa tcgtgtatgt cggacttttg
     7081 atcatttgcg ttatccctaa tccttgggca attcctttcg tcaacgttgt tttacccgca
     7141 cctagatcac cagtcaatat cagattgtca cctggcattg cggaatgacc aattgcctct
     7201 gcgaattgtt ctgtcattgt taatcctgat agttcgatca tttttcatct tctcccgctc
     7261 ttctttcttt ttgccagtat atcgcgtaat gaagtttttt taaagaaaaa atgaactagt
     7321 caagaacagc agctgctgtt tttggctagt tcgcgtgtat ttgttaatca atcaatgttt
     7381 gtgctgctgt gataatcgat aatttatata catcttcttc atttgctcca cgagacaaat
     7441 cagaaaccgg tttgttcaag ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac
     7501 gctgagcaat tttatagccg atatttcctg actgcaattc tggaaataca aagactgttg
     7561 cttgaccagc tacttctgaa tctggcgctt tcagttgtgc aactgaagga acataagaag
     7621 catcaaattg caattcgccg tcgatcatgt attgtggcgc tagtttttta gcgattttag
     7681 ttgcttcaac tactttgtct acttctggcg atttcgctga gccttttgtc gagaagctca
     7741 tcattgctac ttttggttcg atatcgaaca attctgctgt ttttgcagaa tctacggcga
     7801 tttcagccaa ttcttgcgca gttggattca cgttgatcgc acaatccgaa aagacatatt
     7861 tttcttgatc tcttccacgt accatgatca tcgctccgct tgtccgactg atgccaggct
     7921 ttgttttgat gatctgaagt gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag
     7981 agaccattcc gtctgcaatc cccatatacg taagcatcgt accaaagtaa ttcacatctt
     8041 ttaaaatctt ttctgcttgt tctttggatg cctttccatt tctacgttca acaaaagtat
     8101 ccaccatttc atcccactta tcgtaattat cagggctgat gatcgtaaag ctagatgttt
     8161 tgattcctct ggcatgcgct gcatttctta tttcttcttc gttccctact aaaatgggtt
     8221 ccacaagttc ttctgctttc aaccgggcag ctgcgcctaa aatacgagca tcagtagctt
     8281 ccgggaaaac gattttgatc ccacggcgaa caattttaaa tcttaaacta tcaaacaatt
     8341 ccacaagatg acctccagac gtttttatat gtattatcat acactattcc agaaaaaaag
     8401 aacagtttat tttatctgta tcagtcaaaa atgctggaaa aattaagttt ttcacataca
     8461 aagcgaactg cactataaca ggaaagcgct gttttattac actgtttcag gcagttgcca
     8521 atcaatcggt tcttctccta acgcaaggag cgcatcattc gttttcgaga agggtttgga
     8581 accaaaaaag ccacgacttg cagataacgg actgggatgt ggagatgtta tgatgatatg
     8641 gcgactctta tcgatcattt tgattttttc ttgtgccggt tttccccaca agataaacac
     8701 gattggtttt tcccgttcat ttaatttttc aatgatcgtg tctgttaatc tttcccaacc
     8761 tttcccgcga tgagaatacg cttgcccttc ccgaacggta agaacggtgt tgagtaaaag
     8821 gactccctgt ttagcccatg acactagatt tccatgttga acaggtgcga ttccaagatc
     8881 atcataaagc tctttataga tatttcttaa cgatggcggg atctttacgc caggttgcac
     8941 agaaaaagat aagccatgag cttgattttc tccatgataa ggatcttgtc ctaaaatcac
     9001 cactttaact tcttcataag gagtcaattc caatgcttca tagatatgat acatatctgg
     9061 atggatcttt tgggacgcat attctttttt taaaaattct cttagatgca gataataatc
     9121 ttttgaaaat tctgaggaca aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa
     9181 ctccacctcc taataaaaag aataactcac attttcttca atcacaaggg acaatcttca
     9241 ccctcagcac ttgattaata tggtaaacta ggaacaagaa gcagaaagga acgataatca
     9301 ttatgatcaa attaattgca tcagatatgg acggcacatt attagatgcc catatgagtg
     9361 tttctcctga aaatgccgag gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg
     9421 tcgctactgg tcgaaactat caagaagctc gcgcagcttt agatgaggta ggaatcgatt
     9481 gtgcgatgat cactttgaac ggggcacagg tatttgataa agaaggaaat tcattgttta
     9541 ccgtgtcgat tccaaataat caagcaatct ctgttttaga tattctagat gctaacggta
     9601 tttactatga agtagcaacc aacgatggtc tttattctga aaatcaagcg aaacgaattg
     9661 aaagttttgc ttccatggtg gctacccatt tacctcacct tacgtataaa atggcgattg
     9721 ccatggcctc agctaagctg gagttattgc acatcacata cgtggacagt attcgtgcta
     9781 ttttggaaga aaagaatctg gaagttttaa aaattatttg ttttcataca gaagggccag
     9841 ctgtattagg tccagttgga aagcagattt ctcttttagg cgatctagcc gttacttcct
     9901 ctggtcaaaa caatatcgaa gtgaaccata aaaacgctca aaaaggaatt gctgttgccc
     9961 atgtcgctca cgagcgtggg atttctttag aagaagtcat gacgattggc gataacttca
    10021 atgatgtcag catgctgcaa gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg
    10081 aagtaaaaga ttatgccaaa tatacaacgg acacaaacct tgaatctggc gtaggaaaag
    10141 ccatattgcg cgcgattgca gaagacttgt agttttatct agaaaagagg gatatagatg
    10201 tcagaatttt tcattcagga aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac
    10261 gaaaaaggac attccctgtt tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg
    10321 tctctttatg ccatgaatgg cgatctcgtt gctcacatca agcagataag cctgacattc
    10381 ggtacgcgtt ttgagctgta taaagagttc gaaaaggtgg gaaccatgca gaaaatattc
    10441 aattggcccg gtgactttta ttatattcgt agacttcact ggacagtcca aggtgatatc
    10501 tataatcatc aatatcaaat ccatcatttc aatcagcaaa tcatgaaaat ggataaagca
    10561 acactttttt caggagatta ctatgtattg gatgtctcaa atgatgaaga tgctccagtt
    10621 tgtatttgta ttgctgcggt tttagattac tggctctacc gtaaagacaa aagtcgcgat
    10681 aagcctgttt cttaccgact taacttaagt taaaaagatg tacctgaaat gcggaaatac
    10741 gcatttcagg tacatctttt tagatattct tttgcatatg atcataaaga tgaccactga
    10801 tcatccgctg accgacaact ttgaagtcat ggcgttcgta taatttttta gcctttggat
    10861 ttttttcatc cacacttaga ccgatgacgg agcgattagc tttcttcgcc aatttaggta
    10921 atgcttctaa taatcgcgaa ccgatgcctt gtcctctaaa atcttctgat acactgatag
    10981 agtccaaata ccattcgttg ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat
    11041 ctagctgata tttctctaag ataggtacca gtggctgatc gatgattgat tcttcttcat
    11101 ctgtataacc gtaagcaact cctgcaactt taccgtctat ttcttctacg atacctcgtg
    11161 aataactgta acgataattt ggatcagtga ttgcttcttc taaaatctct aaagtctttt
    11221 gttcccctgt ctgcttcaaa aatggcagtt ccatatcttt tagaatcact aagatcaatg
    11281 gggcgatttc tggagcatct tcttttctcg caaaacgaat catagtttcc tctccttttc
    11341 tccattctaa catgcagaaa aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct
    11401 tttcagtaat gaaataacca tgagtaacga ttcttgccgc ttcagttcgg caataagggc
    11461 agacgactgg catttcttta tcattatgtt cgactacatt attgacttct tcgtatgcaa
    11521 atttttttcc gcaattaggg catttcattt tttccatgtt atctcaactt cctttcatct
    11581 ttactctctt agcttactct tatattgtaa aaaagagaag caatatcctt cttctatgca
    11641 gatatccata cctgacggtc ctgttctcca tctattaatc ggactttata ataaggacgt
    11701 tcatctttga ctagaagtgt ccactctttc acttttgctt ttttcccgta gtagctgact
    11761 gcttccttca tcgctgcttg cggttgtttg aactcttccg ttttttctat atcaaaaaga
    11821 tgttctgcgg ccttttccgt gtcatttcgt acggttgttt tcccagtgga tggattgact
    11881 gctatgactt gtccttcaca aaaaaactgg tattcatatg ttgcattcgt aggattctcc
    11941 aaatcttttt caactgcaaa atgaatagca gatatgttat gttcttttgt ttcgtttttt
    12001 acgattgctg ctgctttttt caatgagatc cgatattcct ggtcactaat atcatccatg
    12061 tgaaaagcgg cattgagcag tcccgtcata ccggtaatcc ctattacgat gagggcagca
    12121 acaccaaaca tcattttttt cattcttcat tctcctgttt ctttttttta aagaatatca
    12181 agaaatgaac gaatgaaaaa tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt
    12241 attcgtgcag ttctaatggc aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg
    12301 taaaggtatc ttctctaatc ggttctgatt cgatccctaa ggactgcaat tctgcaattg
    12361 tttcttcaat atcgtctact ttgaaagcaa gatgacgcaa gccacaagct tctggatagt
    12421 ttggtcgttg tggcgcatta gcaattccaa aaatctccaa ttcagcactt cctagtttca
    12481 gatctaattt atagtcatct cgttctttgc ggtaattttc tctgattact tcaaatccta
    12541 atttattaac ataaaaatca cgtgatttct gataatctga aacaatgatg gctatatgat
    12601 ggatcgttgt aaggttcata tgagcactcc tattcttcgt ttaagctcgg atctatatta
    12661 atctccagtt ccattgtaaa tgtgtcagat tgaaaaaaat agctgttttt cctttttata
    12721 agaaaaaagg caaaaaaaac agaaagtagg tcctccaaga cactcttcct gttcccaata
    12781 taataaatga aagtaagaaa ttatattttc aagaaaacaa acagaaaatt atcataacac
    12841 aaattattta taattgaaag actagttttt tttcaagaaa agcatacaat agattgtgtt
    12901 agtaaataca gaacacacta catttagtgt cttaaggagt cctcatacat gaagtcatat
    12961 tttcagtttt tagctcatca gctaaaaggc tggccgcaac aaaattatta tcttttcttt
    13021 ttcagtcttg gctgccaggt catgactctt gtcaatagtc ccatcacttg gctgtccgtt
    13081 attacattta tcggtaccac gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa
    13141 tcagtcaacg gtattttagg tattttgtct gcgatttgtt ttattatcgt tgggttttca
    13201 gcaaagaact atttaagtat cggtgaacag attgcttata tgatcacatt ggatatcccc
    13261 gtattgttaa gtaaagaatg gaatcataat atggcttcaa aaatccgaaa attcactgga
    13321 aaaagctggt ttatcgcaat catcagtact ttccttgtct atcttgtttc aggattcctg
    13381 attggtcgtt atacagatga tcctcggccg tgggtagatg ccattgcttt ttctattagc
    13441 ttgactgctg gtatcatcag tttcttgcgt tacaacaacc aatattactg gtggttagct
    13501 tcagggcttg ctcaaatggt cctttggtat atttcattta cgcatggatc tgcaagtttg
    13561 gcgatgttcg tgaacagctc tgtttacttg ctgaatgatg tccttgcatt taccgtttca
    13621 ccttggtata accaaaaaga acgtgaacgt atgcaaaaaa tggaagaaga atactacaac
    13681 gcacaacaac acgcataaaa aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc
    13741 acgctctaaa cacgtataaa cggcggaatc agaagcaact ccttcggaaa taagctgaaa
    13801 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    13861 ttctgtccca gcctctattt tttatagtaa ttccttcaga tactttcttg cctcatcatt
    13921 gaaacgcagt gattcttcta acttctgata atattccaac gcttgtttct gagcttcagt
    13981 aaaacgataa tgttttcctt tccatttgtc gattgtctct tgcacatttc cagattggat
    14041 atctttcact actagttgcc atttttcttt tgagactgta cggtctttct tatcagcaga
    14101 ttgtatagct tctcgttctt tacgcaatac ttttaaaaaa ccatttctga ctatccgagg
    14161 aatgctgtcg aagaaccctt ctgtaaaacc atagcgacaa tattgcttcc aaataaatag
    14221 atagattttt ggttggatga atcgttggtt tttcgcaaaa tattccatct gagcagtgac
    14281 ttctggtgtt cgttgacgtt ttgtctttct caacacggaa ataaatgttt caaaataagc
    14341 attaggatgt actggcactt cttcatatac gatggattgc caatctttac gtaaaaatgc
    14401 gcgatagttg atcaagaatt cttctacatc ctttactaat tcttctgcaa agtcgtcaaa
    14461 tagttctttt tttatctgtc gcttttttga ctgcccattg atgtgttcac ttattttctt
    14521 gatttcattt tccaatgctt gataatccgc tatgtaaatc ggcatttcta cttggcgaaa
    14581 ttctgcgagc cgctgagcta attttatatc cggtaaatat ggctgtttca tccgacgatt
    14641 ttggtacaag acaaaacagt atttttgcca tagtccttca ggaaaatcga tattcttctg
    14701 cttaagccat aataattcgt ctaaggccag atcaactttt gtcattccct gatggatcga
    14761 agctgccact gtcggagaga catgcaggtg atcggcaaag tggttgatgc ctagttttag
    14821 gatctttccg gtttttggcg atttaacgat gtattgcact tttaattctc taccacactc
    14881 acaatacaat ttttgctggc ttcgttttgt tcgaaaaaac ggattgatct tgaagtcaga
    14941 aaacacccaa tctgttgcag ctaaataatg ttcttccata aaacgagagc tgattagata
    15001 tcggcggtgc ttatcgacta gttcccgctg ttttttcgtt aattttgcat agatctccca
    15061 acgttgactg cttgtgtatt gtttagaaat tttgatggga tagcttttgc tgatttcttt
    15121 tggaaaccat tcttcaaaga cggattgcca ttcttccacg gctgtttctt tcctttctaa
    15181 ttacaaaaag ctggctacta acataacgtt agcaaccagc cagtgtttct tgctaaatta
    15241 gtgagttgtt ccggcatttc cggaaatatc ttctttattt tctactgctg cttcaactgc
    15301 ataagtcaaa gaagaagcaa tgtcttctaa agacatagaa ggcgtaccca ctggacgatc
    15361 gatcacttga tcgggtgaga atggcacgtg gatgaaccca cctcgaattg ccgggaattg
    15421 tgtttcgatc aaatgaagca aacgatacat gatatcattg cagacaaatg ttcctgctgt
    15481 ataagagatg tacgctggta gaccatgatc ttgtacattt tttgtcattg ctttgattgg
    15541 caatgaggta aagtaagcgg ctggaccgtt ttcttctaaa gccgtatcga atggctgatt
    15601 tccttcatta tcagggattc ttgcttctgc tagattgatt gcgacacgtt cgaacgagac
    15661 tgctgaccgc ccacctgctt gtccgacaca gatcacgaca tctggttggt gctgttggat
    15721 ggcttctttg actacttgac tgcttttttc gaatacggtc ggtacttcca ctttgatgat
    15781 ttcagcaccg ctgatttcat ctggcatttt ttttactgct tcataggctg gattcacttt
    15841 gtccccacca aatggatcaa aacctgttac tagtactttc atctgttgtt cctccgtccg
    15901 attgatttaa catatggtta tcttttatca taccattttc cttgaacagc gtaaataaag
    15961 gaaacaatag tttaaataac ataacagatc aaacttaata tattcttata aaaaaatagt
    16021 gttccactac aatgttagaa gaggttgcga caattattct gtcacaaccc ctctcttcta
    16081 ttatttaaat agaatcatat aagcaatttg gaaaactagc ataaataacg caataaacaa
    16141 ctggtttttg atcactccgt atttgtcttt catctccagc atcgctacag gcacgatatt
    16201 gaagttagca gccatcggtg tcagcagggt cccacaaaaa ccgcaagtca atgcaaccat
    16261 tcctattaac gctggattcg ctccatgact aaatacgaat ggtgcaccga tcccaaccgt
    16321 catcacggtg atggccgcaa atgcattccc catgattacc gtaaatatca ccatgccaag
    16381 agcgtaaatg atgatgccga tgttcacatt tccttctgga acgatcgtac ctactgcatt
    16441 ggaaatcact gtcccaactc cagcgcttgt aaataccgca cctagagaag ctaaaagcat
    16501 tggcaacatg cttaacggcc ctactgtgcc cagcatatct gctgcatcat ctaaaaatgt
    16561 acttggtcga ttatctcgag aaaagaacat caagattagc atggctgcaa aaacaccgac
    16621 gcccacgccg actaaagcac ctaaattggt aaatagagca aacaagatcg caaagacacc
    16681 gatactcaaa gctggaataa aaattttcat tcctagtttg tctgacattt tttccatata
    16741 ttcttttgat ggaagccggc tttctccttt gctgactttc ttagcaattg ctggtatggt
    16801 catcgcaaag atcaagccac cgttcacgat tgccggaagc catcttcctc ctgcaataac
    16861 tacccctaag accgtccaga aaaaggctga cccataacga tgaggattgg ttttatctgt
    16921 taaatttctt actccagtgt aaatcaatac gagtcccatc acgatataaa ggatctccag
    16981 taatttatcg cctaataaaa tatcaggatt tgtaaaaaag tccatcttat tgcccctttc
    17041 ctggataata tttcttcgct aatttctttt ctttaagata gaagtagaca cttgccacaa
    17101 ctaaagccgt gatagccact ggaacttcca ctaaagcaag ctgtcccaat gttacgtcat
    17161 atcctagatc tttcagtgtt gattggacta gcaacgcccc tgctcctccg atgaatagta
    17221 cttggccaaa gaaccagcag acattttcca tagctgaagc catacctttt agttcttctt
    17281 catgcttagg atggacttct aacccttttg attcgatggt ccccaatgcc atcggtaaaa
    17341 tgattggacg aacaaatcca gcaactccac caaaactcac gttaaaagcc gcaaagatca
    17401 ttcgaaagat cccataaata tctacaatca taccagcaga tactttcttg aatcgcttga
    17461 tcaaggaagc cgctgattct ttcagaccat ttctttcaag cgttcctgtt gccaacataa
    17521 tgatgataaa aatcgccatc ccgcgattgt ttacaaaact tgtacctaaa agttctaaca
    17581 ttccttgaat gccaagaccg ccagtcaaag ctgtaacaac taaagcaacc attacgatca
    17641 atatcgagtc taatttaaat gcaaacccga gaataacaat caagacgcct aaaaggctaa
    17701 gataattttc catatatttt cctccataaa atgacaatat ttttttaat
//
LOCUS       DAZUMB010000048        17532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000048 DAZUMB010000000
VERSION     DAZUMB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.048"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..808
                     /locus_tag="ACG80L_001447"
     CDS             <1..808
                     /locus_tag="ACG80L_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5023686.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1161..2009)
                     /locus_tag="ACG80L_001448"
                     /pseudo
     CDS             complement(1161..2009)
                     /locus_tag="ACG80L_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2162..3274
                     /locus_tag="ACG80L_001449"
     CDS             2162..3274
                     /locus_tag="ACG80L_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5023687.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="ACG80L_001450"
     CDS             complement(3352..3645)
                     /locus_tag="ACG80L_001450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS5023688.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="ACG80L_001451"
     CDS             3813..4127
                     /locus_tag="ACG80L_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS5023689.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="ACG80L_001452"
     CDS             4148..6127
                     /locus_tag="ACG80L_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5023690.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="ACG80L_001453"
     CDS             6140..6466
                     /locus_tag="ACG80L_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS5023691.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="ACG80L_001454"
     CDS             6482..7000
                     /locus_tag="ACG80L_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023692.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="ACG80L_001455"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="ACG80L_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5023693.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="ACG80L_001456"
     CDS             complement(8436..9941)
                     /locus_tag="ACG80L_001456"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS5023694.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="ACG80L_001457"
     CDS             complement(10033..10623)
                     /locus_tag="ACG80L_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5023695.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="ACG80L_001458"
     CDS             10802..12448
                     /locus_tag="ACG80L_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS5023696.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="ACG80L_001459"
     CDS             12497..13216
                     /locus_tag="ACG80L_001459"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5023697.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="ACG80L_001460"
     CDS             13695..14642
                     /locus_tag="ACG80L_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023698.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="ACG80L_001461"
     CDS             14654..15574
                     /locus_tag="ACG80L_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5023699.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="ACG80L_001462"
     CDS             15615..17072
                     /locus_tag="ACG80L_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5023700.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..>17532
                     /locus_tag="ACG80L_001463"
     CDS             17417..>17532
                     /locus_tag="ACG80L_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5023701.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAA"
BASE COUNT         5499 a         3007 c         3578 g         5448 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gc
//
LOCUS       DAZUMB010000049        17352 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000049 DAZUMB010000000
VERSION     DAZUMB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.049"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1130
                     /locus_tag="ACG80L_001464"
                     /pseudo
     CDS             1..1130
                     /locus_tag="ACG80L_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
     gene            complement(1146..1412)
                     /locus_tag="ACG80L_001465"
     CDS             complement(1146..1412)
                     /locus_tag="ACG80L_001465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023702.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLVN"
     gene            complement(1428..2078)
                     /locus_tag="ACG80L_001466"
     CDS             complement(1428..2078)
                     /locus_tag="ACG80L_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023703.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(2089..3261)
                     /locus_tag="ACG80L_001467"
     CDS             complement(2089..3261)
                     /locus_tag="ACG80L_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS5023704.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(3280..4152)
                     /locus_tag="ACG80L_001468"
     CDS             complement(3280..4152)
                     /locus_tag="ACG80L_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5023705.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(4174..5094)
                     /locus_tag="ACG80L_001469"
     CDS             complement(4174..5094)
                     /locus_tag="ACG80L_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023706.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(5094..5363)
                     /locus_tag="ACG80L_001470"
     CDS             complement(5094..5363)
                     /locus_tag="ACG80L_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023707.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(5360..5740)
                     /locus_tag="ACG80L_001471"
     CDS             complement(5360..5740)
                     /locus_tag="ACG80L_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS5023708.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(6207..7670)
                     /locus_tag="ACG80L_001472"
     CDS             complement(6207..7670)
                     /locus_tag="ACG80L_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5023709.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(7734..10739)
                     /locus_tag="ACG80L_001473"
     CDS             complement(7734..10739)
                     /locus_tag="ACG80L_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5023710.1"
                     /translation="MATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEESKSS
                     EEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATSFSEQ
                     ERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVRWQID
                     GEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNYVLAQ
                     TYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEEVEVK
                     DTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGVGSSF
                     AYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIKRHDT
                     GETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTITIEP
                     NKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAKTDIT
                     APDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQPSEEQ
                     TTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGMTAHN
                     KNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQPITV
                     KNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKYWVTQ
                     FHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYNDKEE
                     EEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFPESTV
                     GETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEKLVAT
                     HIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPKTGET
                     VNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(10902..11741)
                     /locus_tag="ACG80L_001474"
     CDS             complement(10902..11741)
                     /locus_tag="ACG80L_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFS5023711.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            11886..12131
                     /locus_tag="ACG80L_001475"
     CDS             11886..12131
                     /locus_tag="ACG80L_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023712.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            12194..12616
                     /locus_tag="ACG80L_001476"
     CDS             12194..12616
                     /locus_tag="ACG80L_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023713.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(12710..13027)
                     /locus_tag="ACG80L_001477"
     CDS             complement(12710..13027)
                     /locus_tag="ACG80L_001477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023714.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(13054..13260)
                     /locus_tag="ACG80L_001478"
     CDS             complement(13054..13260)
                     /locus_tag="ACG80L_001478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023715.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(13241..13423)
                     /locus_tag="ACG80L_001479"
     CDS             complement(13241..13423)
                     /locus_tag="ACG80L_001479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023716.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(13437..13787)
                     /locus_tag="ACG80L_001480"
     CDS             complement(13437..13787)
                     /locus_tag="ACG80L_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023717.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(13813..14115)
                     /locus_tag="ACG80L_001481"
     CDS             complement(13813..14115)
                     /locus_tag="ACG80L_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023718.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(14118..14351)
                     /locus_tag="ACG80L_001482"
     CDS             complement(14118..14351)
                     /locus_tag="ACG80L_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023719.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(15255..15785)
                     /locus_tag="ACG80L_001483"
     CDS             complement(15255..15785)
                     /locus_tag="ACG80L_001483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023720.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(15782..16897)
                     /locus_tag="ACG80L_001484"
     CDS             complement(15782..16897)
                     /locus_tag="ACG80L_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5023721.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(16903..17301)
                     /locus_tag="ACG80L_001485"
     CDS             complement(16903..17301)
                     /locus_tag="ACG80L_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5023722.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4767 a         3553 c         2738 g         6294 t
ORIGIN      
        1 atggaaacaa atggtctttc tatccaagaa actgctaaaa aatttaatat cggttcaaat
       61 agaactctaa gtaaatggat agagcaatat gaagaaggcg gtgcttcttc acttgagagc
      121 caaaaaaggg gcagaaaaat tagtatgaat tccaagctaa acattcctaa aaaacttaaa
      181 gatgagtctc ttgaagaaga agttattcgt ttaagagcag agaacgcata tttaaaaaag
      241 ttagaaacct tgattcaaga acaagatttg agcaagaaga aatcaaggtt aaaacaatcc
      301 ttcagctcaa aaaagaattt aaattgaatc tattactttc aattgcacaa ttagcgaagt
      361 caacttatta ttactgggta aaaaaattag ataagccaga taaatatagc aagataaagc
      421 aagaaattac agcgattgta aaagaatcaa gaaactctta tggttatcgt agagtcactt
      481 tagcgttaaa gatgaaagga tatacaatca atcataaaac agttagaaaa ttaatgtccc
      541 aaatgggact tacctgtcaa atccgaataa agcgatataa atcttataaa ggaacagtag
      601 gaaaaattgc caagaatgtg ttaaaacgaa atttttcagt agatacaccc aataaaaaat
      661 gggtgacaga cgtcactgaa ttcaagataa aaggaagaaa aatctatcta tctcctatac
      721 ttgatttatt taacggagaa ataattagtt atagtatatc aactagtcca acatataaac
      781 tgattgaaga aatgcttcaa caagcaatta aaaaaaaggg aactgagggt tcactgattc
      841 tacattcaga tcaaggatgg cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga
      901 ataacattat tcaaagcatg tctagaaaag gaaattgctt agataattct gtaatagaaa
      961 atttttttgg tgtgcttaaa tcagagtttt tttatcgaga aaaattccga tcaattgaga
     1021 tatttcaaag taaattaaat gagtatatta ggtggtataa taacaaaagg ataaaactaa
     1081 agttaaatgg actatctcca gtagaatatc ggaaacagtc cattaaatag ttcaactttt
     1141 ggggttcagt tcaccaaaaa ggcaagtgct tctttgtagg cattttgact gtattttttt
     1201 gataaagcat gacaaatttc ttgataaaat agttgactgc ctaccgaata ccgattattg
     1261 tgtatccgcc acaaaacgaa cacactgatt tgaatcgacg ggcgtaaaaa agaaaaattc
     1321 tttccataag aaaaagaacg tccttcttgg gtttcttttg cttgttctgt tggtacttct
     1381 tgtacctctc ttattgattc ttgtctctcc ataaccttct ccttttctta ttcttctaaa
     1441 agctgttgat taaagctact tcccaaattt tgcataaacg tgattttctt tgacgctgga
     1501 tcaaaggtta cgtcaaaaat attcatggca ttattccagg aactatcacc aattttattg
     1561 cgatacgaca ccacaacgag cccttggata gtcgcttttt gtgtgctttt taaataaaca
     1621 gaacatttct ctaattcaga agacaccccg tttgaatcac cataggaaac gccttctttg
     1681 aaatattgct tcatcagttt gtcagaaaga tatggtgcac aattgttttt tcgttcttga
     1741 aaagaatcag gctcataatt aaacagaacc tcgaacgctt ctgtcactgt tttttgaaag
     1801 gtttgaatgt ttcctttttc tgttgaatca ttgtttttca attcttcatt tgcttttttt
     1861 aacgtcgtga cctcgctttc tacttgttga agttgttctt tgagtgacgt tacaaggtct
     1921 tcctgtcctt tcttctcttc ttggaacttg tttatctcca ccgtcagttt ctcttccttt
     1981 gctgttagct gtagcttttc atgaataaga aaaaatgtac tgcctacacc aaataagaac
     2041 aatccgccta cacaaacata aataaatcgt ttcttcattt gacacctctt atttctttat
     2101 cggtcgagca aaacaaatat caccaccatt aagcaaatac ccaaaggata aattgacttg
     2161 cccttcacct acaccgttag tattcattcc cccttgttcg attactttcg tagaaagtcc
     2221 atgccaatct tccgctaaaa cagctgtgtg tccatcattt cccacaccag aacctaagtt
     2281 cacaatgata atatcgccag cttttgcctc gctctcaggt atttcttgta agtattgctt
     2341 acttcctctt gcgtcgctcg tcattgagcc ggtaaaccac ccaacgttcg caggcgtttt
     2401 ataccctgct ttcgttaaaa caagccacac aaagctcgaa caatcggcat agccattttt
     2461 atctggattt tcgacactac caaattgtgc ccgttgcggt tgtgaataat gaaaataacc
     2521 aatcagactt tttgctacgt ttaatacatt actatcggaa gaactgcttg ttcctgttgt
     2581 ttctagacaa taatcggtca agtctttact tcctgcgttt gccccatttt ctaatgtaat
     2641 tgaagcgacg ggatcactgt cattctttcc tagttttgcg taccattgat tcgcaaaagt
     2701 aatacgattc cccatcattg gtatccctgc acgctcaaat ttcacttgcc actgttctgt
     2761 tgcttcctct acgctttttg cttgtaatgc aacttgtgaa gctttttctg ttgtgtttag
     2821 ttcatgaatc aaaaactcaa cttgtacacc taaatctgac cactctttgt ttcgctcttt
     2881 ggcaaaattt ttcaaagcag tcgttcgccc tgccgtccat tgtgcaatcc catgcccaga
     2941 taatggatcg tttggtcgtt caatcgctgt gggactcata ctcgactctt gttgaaaatt
     3001 gcctaacata cccgctagac cctctaatgt cgtatttgga atatgttttt gtacttcctt
     3061 tgcaatttga agcccgtttg tctccatgcc ttggttcgtt aatgagatag tacttgcgac
     3121 aatttcggtt ggactacagt cgatttcttc ctcttcagaa ccactactaa tcaaaagtat
     3181 cataaacaga aaaccaaaca tacatagtaa tagtgtgggt agacaaccga acccacacca
     3241 taaaagaagt tttttcttca tgtttgatcc ttcattcgct tagttcattt tttgtgttgc
     3301 ttttcttttg cgccaaaaag caaaaccgct tcctgaagcc aataatccac caagtgctgt
     3361 cgcaacgaga gacacttctt cgccagtctt aggcaatact ttcatttttc catcagaccc
     3421 tttcaccgca atatttccgc ttggaagttg ttcgaccttg tctgaaatgg gcgtcaagcc
     3481 atttcctgat tctttataag cgacaccatc aacgacggag acaatttttt ctcctgaagc
     3541 tgtttcaatt ggtgtacttg gtgttggaat atgggttaat tcagacgcat tattggtttc
     3601 aatttgaaca ttttgtccac ctgtttgttg gctgccaata cttccatctg gttttactac
     3661 gacgtcctgt actggaggcg tagtgttcgt ccccccatta ttggtaggtg gtgtcgttgg
     3721 aggagtcgta tttccttcat tgcctcctgg attcgacggc ttttctggtg tctctgtatt
     3781 accaccattt cctgggtttt cctcaacggg tggaacttca ggtgttcctt cccctccgcc
     3841 agtatctgga ttttctggta cttcaggcgt ttctgtgcct ccacctgtat ctggattttc
     3901 ttccgttggt ggaatctctg gggtttcagg agtttctggt atttcgggaa tttctggggt
     3961 ttcttctcct ccatctgttg gtggtaaaac aggttcttca ggaacttcag gtgtttcttc
     4021 cgttggtgga ataatttctg tcggtggtac acttggatca cctggtgacg tagtatctgc
     4081 atacgcactg gttcctacca tacctagcaa tacagttgca ctaaataaac ttcctaaaat
     4141 catttttttc atgtgttttt cctcttctct tatttattga tttatttcaa aatcatctag
     4201 ttcgggaagc gtggaaacta gataattttt ttcctgttct tccaattgaa aagacacttt
     4261 ttttgtttct tgtgtctttt tctcggtttc caatgtaatg acatacgcaa tttgccaatg
     4321 cttgccttct ttcttcattt cccaggataa aatacttttg actttttcct tttgaggact
     4381 gatttgtttc gcaacttttt ccgttagaaa cgattgaagc tttttcctat accgttcttc
     4441 tgccatttca cccgaataat aattcgtaat aaaataacga ctaaatgtat caatttgtgg
     4501 ttgttttgtc gcaagttctt ccgcaaatgc aatgcgttct tgtaactggt gctccttttt
     4561 cgttagttgt tcaatctctc tttgcgttga aagttgcata ataaaaagac cacaagatac
     4621 aacgacgata aatccaaaca aaagccattg aaaagacatt tttttcttct gaagagagcc
     4681 gttgctttgt gtaaacgctg tttctacaga aagtggtgtt ttcttttcct ttttgatttt
     4741 tgttggtcga cgttcgttct tttggtcttt ctttacttga taaaacttcg gtaattcgga
     4801 atttaaggtt tgaataaagg tttgtgcttc ttcacttaat tgcccatttt ctgttaaaga
     4861 ttgttcaatc aatgaaatca aatgaaactc ttctactgga aaatcatacg agccttcaaa
     4921 tactggaatt tcatggtttt ctgtgtagat ttccaaggta caccagccaa tttccgctcg
     4981 tgccaatagc tcttcatttt ctcttttcaa ttgaataaat gccttatcta atgcaatttt
     5041 tttatctgtt agtaaagaca tgttatcaac ttctgtaaat cgaatcatca tcattagtca
     5101 aacacttctt ccaccacttc taccgcatac accacttgaa tagggttatt taaagaaaat
     5161 ttcacccgat attcttgatt gttcaaactg acgatatagc tatcctcatc ttcagcataa
     5221 taggtatatt cgttgccttt gacttgaaaa tatttttgga tcgcttgtct ttgttgccat
     5281 tcgccaatct ttggaaaata ggcgaaatga tagccaaata aaacgcctat ccctaataaa
     5341 acaattcctg ttactataat catctggttt cctcctgtac ctgtttatct tctattgact
     5401 cttttgtcgg taagcaataa agaaccagta acacaagaga gccaatcgtt gggacaaaat
     5461 agaaaaaaat aaatgcccaa tgaaaacctg cgtctcgtaa acgtcgaacg ctgagagaaa
     5521 gagacgggac aagaactacg aaacaataca caatcaatac gcctatcaaa caaccaatta
     5581 ttgtttcaga agcattcaac gctcctgcta gtccaatcgc taagcctagg ccaaacccaa
     5641 tgagggcatt cattaacgca acgagccaat actcttttct tgtggctttc cctttgtaat
     5701 taaagctatt ctgccaaaat tttttataat ttgtgatcat atctttgtcc tttctaatgt
     5761 ttatttttga ttctagtttc ttctgtttta ctaacaatga aacttgccat aaaaaaggtg
     5821 gccaatacac caccagctag aaatccactt acaaataaca aaattttttc ctcctttcta
     5881 aaaaatttcg ccataaaaag gcaaagtatg aacacgataa ataccatgta catactttgc
     5941 ctttttatgc aatatatgtt aactctccaa gcaattactg atttttaaat tctttataaa
     6001 taaaataaaa atcgtttgag taaagaaata agtcaatgaa gacccaaacg ataaaaaata
     6061 ttcctaatac tatatttaat aaactacgtt ctagatagat acgagatttc aaattaaaaa
     6121 acgaaaatga gagatacaaa tatatcaaat atttaaaaag taaccacatt ttctatacca
     6181 ctccttatca gaaacagcct tattctttag aataagactg tttctgggtt gattaatttg
     6241 tcttttgtta aactaccatc taaggcaagt tctaaatgta aatgtggtcc tgtactattt
     6301 cctgttgaac ccacataacc aataatttgt ccttgttcaa ctgtttgtcc tacgttcacg
     6361 caatattcct gttggtgcgc ataaagagcc acacttccgt cttcatgctc aattgtgaca
     6421 taatttcctg acaaagtatg atacgaagct tctatgaccg ttcctgcctt gctcgcatag
     6481 attggttctc cttgattggc tgccatgtct aatcctctat gaaattcttc tcctcgccaa
     6541 ccgaaggaac tagaaattga ataatttttt agaggtacca tatagccttc tttgcttagt
     6601 tgtggaccag ctacttcttt atcatatttg attaagtcat aggtttcgat taacccattt
     6661 aattttttat cgtagctagt gtctgttgcg tatcttcctg ttaaaaattt agtcgcttcc
     6721 tgataggttt ttgccttact tttccaaaca ccatcataaa aagaggtatt tccaactaag
     6781 ccttctttta acagtttggc atagtctttt aagctttctt catagctctc atattgccga
     6841 aaagttgctt gggtcgtata aagtgtccca ttgcctaaat cttcttgtgt cgcaaaagag
     6901 acgccctttc cgttatgtgt gcctttgatt ccaaatagat tgtagtttgg tgcctgagcc
     6961 aattgacttt gtccgcttgc ggattctaaa atggcttgcg cgatcataac agaagcgtat
     7021 aaatcatttt cttgtccaat tttacgagca gattcaccga ttttacggat aaacgtttct
     7081 acagacgcgt ctttttcaaa atgaatagtc ccttcgttat cttgtgtgct ggaagggact
     7141 aattcttgta tcggtgcgct tggtgcttgg tggtagtttt ttgtctcttc tgaaccgatt
     7201 gattgtgcct gcggtacgtc attcataggt acagtcacac tctgttcagg tgccttttgt
     7261 gggcgttccg tttttgttgg cggttccgct tctgtttcgg tacttggttt ttgaggagtc
     7321 tgtgagcttt catcagaagg ttgctcattt ggttcttctg acggttcctc ctcaggttgt
     7381 tgattgtctt ctggtttgtc tggttgattc ggtgtttctg gttgttcttc agaacctgtt
     7441 ggtggagcgt tcatttctgg attatctggt gtatctattg tatcttcagt cggtgtttct
     7501 ggttgatttt cttctccagt ggaatttgtt gtcgtttctt cagtgatcgt tgcagattgc
     7561 gaatttgtca gttgattgtt gccttgttca ttagccaaaa ccgctggcgt atacccaaat
     7621 acttgaccta ctaataacaa agcagataaa catttactcg tttttttcac aagattgcct
     7681 ccattcttca atcaatcatc tagagacagc tgattgctgt ctctagtcat actttaattg
     7741 ctttgtttca ttttacgacg aaccagaaca gcccccacac caacaaaaag aaacagtccc
     7801 aatgcaatta acacatcgtt cactgtttct cctgtttttg gtaagctttg ttttggtgtg
     7861 ctcgtcggcg tttgtttgac aggggttgtc ggcgtttctt ttttaatgcc ttttgctgtc
     7921 aatgtttgtt tttcattctt caaatcaaag tgacgtgctt cttcctcttt atggtcttta
     7981 tccttataga caatatgagt tgcgaccaat ttctccccgt cttttaacgt gcctgctttg
     8041 tagtcaaagg atactttaaa ctcttcagac gctttagaaa ctttacgtgc agtttctacc
     8101 gtaccaacga ctgtatcttt tccattttta tcaatcttat ggaattgagt cacccagtat
     8161 ttggtttccc ctaccgtaga ttctggaaat tcttgcgata catgatcgat caactcttgg
     8221 tctttgtctg gatcaaatgt ttggtcgcca ttcactgtaa taaagcgtgt ttcgattttt
     8281 ggttctttcg cagtcactgt ttgatcttta ttggttaaat caaaatgacg agcgaattct
     8341 tcttcctctt ctttgtcgtt atagacaatg tgcgttgcga caatcgtgtc accgtctgaa
     8401 accatgtctg ctttgtagtt gaacgtcaca tcaaatgttt ctttttcatc ggtcactttt
     8461 cgtgtttctt ttaccgtatc taatacagtg acttttccat cggcagtaat tttatggaat
     8521 tgcgtcaccc aatatttctc tgtcccaacc gcagatttag ggaatacttg ggacacttta
     8581 tccactaatt cttggtcttt tttcgggttg aaatcttttc cacctgtcgt tgtcacaaac
     8641 aaagtagaaa tttctggttg gtcttcttct ggaaccacga tttcttcgtt tttcacagta
     8701 attggttgta cttctccgtc tttgaccgtt actaaaatcg tttctgtttg tcctgacgct
     8761 tgcccataat gctcagggaa cacaatttcc gtaaatttat acgtaccgtc tggtaatttt
     8821 agtttttctg gttcttcttt tgtttcatac acaaaggaca catttttatt atgtgcagtc
     8881 ataccttcaa tcaggaattt cgctcctggt aattgtagat gattgattga atcgacttta
     8941 ttgaagtcta cttcacccgt ttttaacaag ttgtcaatca catctaattc aaacgtaagc
     9001 acaggcgtcg tgttatcgcc ataggccaca gagaacgtgg tttgttcttc agaaggttga
     9061 cggtagttgt ctggcacatt tttttcaatc aattggtatt tgttttgatc gccattgtag
     9121 aattttttgt ttgccttcca gtgtccgtct ttgtcagttt ttactgaatc caagacttct
     9181 ccttttttca caaggactgt ttctttatct ggcgcagtaa tatctgtttt ggcgataata
     9241 tcaaattcga tattttctgc aggaacggta tcataaacag gtttgccttg attccaggat
     9301 tcttccgcat tatagacttc tttgactttc tttccttctg gaatgaactg ttgtaagtca
     9361 ttttttgcag ttaacgtcac ttctttgttt ggttcaatcg taatcgtatg agtttgtttg
     9421 tctagaatat acccagctgg tgctttgact tctttgacat ccaatttatt ttgaacatat
     9481 tttccgtctt tttcatagaa tgcagggatt tcttttaagg cgatttcccc tttggcatcc
     9541 gttgtatatt gttttgtttc tcccgtatca tgacgtttga tttcatacgt cacatttgga
     9601 acttttttcc ctgtgacagc gtctagtttg gttagtttga agtcgccatt ttttagaact
     9661 ttaatagtta gtccagctct attaggatct ggcacataaa gctttcctag attttgagca
     9721 acaggatcta cataggcaaa acttgaacct acccctctag aatttggatc cagtgaagaa
     9781 acagaaattc gtccgtcatt tgagttttga tttgctttta taaacaaact atttcctgat
     9841 tttcttactt gaacgttagc tgtgttttca acaaccgacc catcccattg agaaaacaca
     9901 ccttttgtat ccttaacttc tacttcttcc cctactttaa ccgtcactgt ttggctatca
     9961 aaagaaggat cttcaagtaa attgtttatt tttctatcta cagtctgttt gaaagcttca
    10021 taattagatt gtccaatagt tccataatta acaaatgaac cacctaaaac ttcccaaata
    10081 tatgtttgtg ctaatacata gttatccatt gaaccactag aatagtagcc aaaataacca
    10141 attaaagaaa tcttttttct aatagcactt gtcgtaatta aattttcgag tggtgtcaca
    10201 tcatattgaa tacccgcact cgtgattgcg ccttcttgaa tgcaaaaaac atcttctccg
    10261 tcaatttgcc agcgaaccat gtaatcatga gtatacattg ttccttcagg agtaattcga
    10321 gtcatattca catctttacg accattttca gtcaatgtta gattggtagc acgtgtctgt
    10381 gcattctttt ccaagattgc ttgccattct tcagattctt gcgccaatcg ttcttgttca
    10441 gaaaaagaag tcgctttttg aatatcttca atggttataa agtatttctc ataaccttca
    10501 gggacctctt gagtcattac attgggcgga gaaacatctt ctgtcgtgtc ctgcgtagtt
    10561 gtctcttctg ttgtttcact tgtggtttca gtactgatcg cttcttcact actttttgat
    10621 tcttcagtct tcattacttc ttcagtagaa tttgattcat ttatcgtttc tgcaaatgcc
    10681 gttacagatc ctaacatggg attgagaaat gtaacgaaaa ttgccaacca agttgccatt
    10741 cttttaaaca tatttttttc gttcctttca ttttattttt cagtcttcta aagtaatttc
    10801 ttatggactt cgacaccgaa acatttttat cctcctcaaa acaaaaaaag actagaactt
    10861 ataattctag tcttcatcaa ccacttactt ttcttttatt tttagtagaa atctacagtg
    10921 tgtttttgtg acccgtcact gtaaataata acatccacca taaaaccttt gttgtaccac
    10981 tgcgaatttg gatcgctaac ctctgcatta gcccaatcat aggcttcatc tacagtgtta
    11041 aatacctttc cactattacc aatctctcca actacaattg ttggctcttc aggtgtcgtt
    11101 gtgccaccat ttccgccttg atcgcctcca ccaggattgc ttggttcttc aggcgttgtt
    11161 gttcctccac ctgtgcttgg gttagaagga gttgtttctc ctccagtatt tccaccatta
    11221 cctggttgtg taggtgtttg agtagtgcca ccattgtttt ctggtgtact tggcgttgtt
    11281 gtaccaccag ttgttcctgt ttgatctcca gctggtgttg ttggtgtcgt tgtatttggt
    11341 gtcgtttgtg tatttgaagt cgttccttgt cctactggtt gattcggatc aactttgtct
    11401 ttctcttgaa tcactgtttg agccacagtt tcacctgcgc tgttttgtac acttgctaca
    11461 ttgccattaa atactaattt atttcctgca tagattaaat ccacatttgt aatcttattc
    11521 atgtccgcta atttttggac agtaatattt gttgcttgac tgattgcgct tagggtatct
    11581 ccccagacaa ttgtgtattc attcccttta atttctgctt tgatttgttc cgccgaacga
    11641 ggtgtccaaa ctttgttttc tgctgcttca gcattcgttc ccacagcaag actaactcct
    11701 ccgactacga ttcctagtgc caatactttc aacattttca ttgtttttcg cctccgattt
    11761 gaataaattt atttacaaaa acatagtacc aaacttatgt ctatcagtcg atatattttt
    11821 tgtagatttt tttatttttt gatatactaa ctttggtact aaaataacta aaaggaatgt
    11881 cacacatgca acttaataat ttaaatgaaa tacatgattg ggctcaaaat aatttactta
    11941 atcgtaaaca agctgccaaa atcactggac aaaactatga tgctttttct agctctttac
    12001 gtagaaataa tatcaaacca tttctagaaa taggcgacga acctggtcct tccatcattc
    12061 gcttatatct aagagaagat attgaaaaat acgcaaaaca gctcgaagaa aaaagacaga
    12121 aacgtcaatg aggcgttttc tgtcttggat ttacaagtaa aacaataata aacactttta
    12181 caacggaggt tctatgaata agaaatacat tgtttcagtt ggttcaatcg tactgattgt
    12241 actcgctatt attgcttttt ctgtatccaa caaaacagaa acacagccac aaaatacggt
    12301 tcaatcgact actacaagtc aatctagcga tcattccgtc tatccttcac taaatagttt
    12361 aaaagaaacg catggacaag atttaattga tcttggttct tctcaaatta ctactgtctt
    12421 atttgaaaag aaaaatattg cgtttattac tcctaaagaa ggaactgacc ttgaattttc
    12481 tatcaaagta acaaatacca aagaaaattt attctccatt cctgacattc aatatacccc
    12541 taacaagtta aagattgggg atacgtttgg tttggctaaa gtaaatgaac aatactttgc
    12601 ttactcactg aaataagttt ctaatagcaa gaggaaaaac cctcttgtta tttttatcac
    12661 ataatcattt tataaaaacg cttcgaaaat atcaacattt ttgaagaaac tatttttcta
    12721 atttctcaat ttcttccaag ggattttcgc taatgaccca tacaggcaca gattgctctc
    12781 gaggctcccc ttcttctaac caccaacctg cataatgaac ttcttcctcc tcagcattac
    12841 catgatacca accaacaaag gattcatcta caacgaatga aatttcttct tctggatcaa
    12901 ataattcatt tttccatgct tcaattgctt gttctttggt gtacttttgt ttgttaaata
    12961 caccacaaat ttcgtcatct acataaaaaa tttcaaaatc aaaattactc gttcctttta
    13021 ctggcatctt aacttttcct cctttgactc acttcaatag atataccact catgttcatt
    13081 ccattgttta ctagttaatt ggagttttat cttagggctt tgtgcactct ttccaaaaac
    13141 ttgagcgtcg tttttttctt ctaaccaaaa aaggtaattt cttacattcg gtactaaatg
    13201 tttttcctgc atttcttcat gaaaaacata taattctgct tcatttttca accaattcat
    13261 tattcattcc ctcattcact tcttcatacc gccattcaga tagtgagatt tctttttgtt
    13321 tctccacttg tcctataaaa atcgctgtat tcaacaagct gatcacactt aataataaag
    13381 caatgaaact aatggttaca cttactcttt cacattttgt cattttgttt ccttctttac
    13441 tcgtttgtta tttcttgtaa taggtctaat aactccttca ccgaaatttg caaatggttc
    13501 gcaaaaattt caataaaagc actcgcacac attaccagac cttcaactga tatatcactg
    13561 cgacaataaa attctggaga aacactgatt tctgattctt tcttacaatt gaacgaaaaa
    13621 actatttttg aatcaaattc cggtaattct tccacaacta tgttttggga ttctaccaag
    13681 aaagggagtg cctgttctaa tgcttcttct ggtgtttgag cagatagaat aattgcttct
    13741 tgttctttta tttcattgtt ctcaaatact ttataacttt ttttcattgt tagttcctct
    13801 ttcattcctc tctcaattag ctaagccgtt actggtacgc cattctctaa atttagtatc
    13861 gctaatcctt aatgctcgtc gaatatcaat cattcgataa cctattgcac gaagttcttt
    13921 atatctttta gcagttagcg tctctaacgt catttcaggc aatgggttcg aatcaaaaat
    13981 agaattcgaa ccgattattt gggccaattg ttgtacttgt aacagctctt ctgaacttaa
    14041 ttcagtgatt gctcgattgc ctatcattgc atacattttc tgtcgaatat acttcttttc
    14101 ttgtttagac aacatttcta tccactccta ttttcattat catttgttac ctccacttca
    14161 gtaaatccac aaataaatga attcatattc caagtaccat tcccttcaac tacaactttt
    14221 ctcctgtcct ctttcggata ttcaagaatc agaccgttta ccaatatgtt tttcactact
    14281 actaatttgt ttgtataccg tggaattgct gttccgatat acttcacttt atttcctacg
    14341 ctaattgtca tttcctcact cctattcttt tttatccaaa actttttcct gtcctattcc
    14401 tgccaacgaa tatacttcac tgcatgctta aacaaaacaa aatgttgccc tgttttattt
    14461 ccgtctttta cttgttccac atacagttca tatttggtca caccttttaa aacgccagat
    14521 actttttttc atccattgtc acaattagtt ttttcatcaa tcgattgata ctgcactgct
    14581 catttttctt gttcatatag tattcctttc tttttcaaaa ctagcctctt ttgtttacta
    14641 gttttcatca atcatcaacg atttaacaga ttttttcttt cgcagggttt ctagcgtcag
    14701 caaccccaaa ctctaaaaaa gtgcctttgc tcccttgaaa aacgagcgga cgattgtact
    14761 cgtcatcata tgtttttcat actacctgat ccacgcaaat aacctttttt tgttctgtgc
    14821 cacacccatc catacgtatc gcttgagatt caccatactt ttttaggtgg tttatccttc
    14881 ctaagtccct gttagacacg ctgattaact tagaaagtcc tgtttgtatc caattttcaa
    14941 agatctccca aaagaaataa gtaggtttac gtgtatcgct tctgttcaaa gtacctgttt
    15001 gtacttgagt atttctactc ccctcgttca agggattcct tccaccagct agcttttcct
    15061 ttcatcatca aatgttcctg aaaagcattt acgtttcgtg tccgtgtgct tacttattct
    15121 tttttcgata gcgattatcc gctcgctact cgctatttta tactttgttt gttagctcaa
    15181 ataaaaacta acaaacaaaa aaggctagtt tctttccgtt ttattttttt aactgtcgat
    15241 aaatagttgt tagtttaatt ccaagttcta aaaccaatac gcccaacaaa accacaggaa
    15301 aaacaattgg ggcaaacaag taaaatcgga tagttttcat gtcttccctc tttctttttg
    15361 gtgtcgctgt ggtcgttctt gccgttcaat acttcgatac attttttatt cattatttgc
    15421 tcgttttgtt ttcctctgga ctgcttttct tgctttttga atactttata cgcttttcgt
    15481 gcctgaatta ataggtcttg tcggcgtttc attcgtgaga aaacaatatt tgaaaccatc
    15541 aaatcctggt tcgaacaacc acgattcgca ataattttcc catcaaaaaa aatagttttc
    15601 acttgagaaa aaaacagttg gaaatcttgt tttgttgcta atttttttgt caaaatcact
    15661 gtgcttttcc ctgttgaatc tacaatgatt cttcgaacaa tccgattcga taacttccaa
    15721 cgttgccgat taaacaatgt tacttcacaa caaatatgac gtaagtgatt aggggtcatt
    15781 ttcatgtgtc caattcctcc aaccaattat gcatcgaaat tttgggcaat tgtgacagcc
    15841 cataaatctg cgtttccagg tcttcataac tacaagaacg cttggcttga agtaatgaat
    15901 attctgcttg atctgatagt tttgttgcca ttggatctag attcaattct cttacacaac
    15961 attcccaaaa attcaatagc gctctaaaat aaaatccttt tcgattctga attgcttttc
    16021 cttcatgacg agcaattttt tcattttgaa tacaccgttg atacgttttg cttaaatctt
    16081 ctgcaaatgt ttcccctaaa attcgatact cgcttccatc agataaacca tgtgtttgat
    16141 tatactttcg ttgccgcttt tctataattc gtttactgtc gtatatcatg tttagctgct
    16201 ctttcgcttc acgccattca gctgaactgc gaacaatcca tgtcaaaaca tctttcggaa
    16261 caaacgtact ttcgctgtaa tgagcaataa actctttttg ccaatcatca gccatttcaa
    16321 caggactagt cgtatcgatt tttgtatcta gattttctgt atctagattg ttagaagaat
    16381 tattaaataa ttgtgcagaa ttttcacatg gatttttttc gtcaaaatca tcgaaaatcg
    16441 ctaaattatc aacagattcc tcctcttctt ttccttgtgc agaatctaca ctggttgagt
    16501 caacaggacc taatgaatac acatcatttt ctgtcgtatc taattctcct agatacaacc
    16561 gagctttgtt tctttttcct gtttgttgat caaatcccat atcctctatt tctagtagct
    16621 catattcttt caattctttt ttgattgtat taacagaacg tctgcaacaa ttcaatgcat
    16681 tggctaattc ttgttccgta aattcaaaat aaacaaaacc ttgactatct acttgatttc
    16741 gttgaatggc taaatctaag cgtgaccgaa aaaccatata cgcgattttt gctttatcag
    16801 acaatgagcg atatttttca cttgtaataa atacctttgg aaattgatag tataattcat
    16861 tgtatttacg agttgcggta aatctttttg ttgccatcgt tattacacct catcttgtcc
    16921 atatattaag aaagaatttt ggtgttggta gacacgaaca tcttccattg tagcaatcaa
    16981 ttcttttaga taaattcgac cgaattcaat tgttagtaag tcgtaatctt ctaattcatt
    17041 tttgatttct tcaatttctt cttcagacac attgagtaaa atggctaatt cttgattggt
    17101 ataagaaata tagacatgtt tcttttcatc aatccgtttt tcaattaagg attcttctaa
    17161 gtactcattc agtaagctat aggcgtattt tgcttgtgca gtcatcgcat aatattttga
    17221 cgattttata aagaatagtt ttggcatatg aaaagttgct tgtgtcagca catctgttgc
    17281 gcgaaatttg ttttttacca tattttttcc tcctagaaaa acacgcaaaa aaagctctgt
    17341 ataaatcgaa tt
//
LOCUS       DAZUMB010000050        17276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000050 DAZUMB010000000
VERSION     DAZUMB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.050"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..540)
                     /locus_tag="ACG80L_001486"
     CDS             complement(19..540)
                     /locus_tag="ACG80L_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS5023723.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            739..4431
                     /gene="nifJ"
                     /locus_tag="ACG80L_001487"
     CDS             739..4431
                     /gene="nifJ"
                     /locus_tag="ACG80L_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS5023724.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4620..5507
                     /gene="cdaA"
                     /locus_tag="ACG80L_001488"
     CDS             4620..5507
                     /gene="cdaA"
                     /locus_tag="ACG80L_001488"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS5023725.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5504..6673
                     /locus_tag="ACG80L_001489"
     CDS             5504..6673
                     /locus_tag="ACG80L_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS5023726.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6697..8052
                     /gene="glmM"
                     /locus_tag="ACG80L_001490"
     CDS             6697..8052
                     /gene="glmM"
                     /locus_tag="ACG80L_001490"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS5023727.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8124..9929)
                     /gene="glmS"
                     /locus_tag="ACG80L_001491"
     CDS             complement(8124..9929)
                     /gene="glmS"
                     /locus_tag="ACG80L_001491"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS5023728.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10338..10628
                     /locus_tag="ACG80L_001492"
     CDS             10338..10628
                     /locus_tag="ACG80L_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023729.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10641..11666
                     /gene="sppA"
                     /locus_tag="ACG80L_001493"
     CDS             10641..11666
                     /gene="sppA"
                     /locus_tag="ACG80L_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5023730.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11669..12328
                     /locus_tag="ACG80L_001494"
     CDS             11669..12328
                     /locus_tag="ACG80L_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5023731.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12485..13417
                     /locus_tag="ACG80L_001495"
     CDS             12485..13417
                     /locus_tag="ACG80L_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023732.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13571..14860
                     /gene="tig"
                     /locus_tag="ACG80L_001496"
     CDS             13571..14860
                     /gene="tig"
                     /locus_tag="ACG80L_001496"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS5023733.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15107..16357
                     /gene="clpX"
                     /locus_tag="ACG80L_001497"
     CDS             15107..16357
                     /gene="clpX"
                     /locus_tag="ACG80L_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS5023734.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16440..17027
                     /gene="yihA"
                     /locus_tag="ACG80L_001498"
     CDS             16440..17027
                     /gene="yihA"
                     /locus_tag="ACG80L_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS5023735.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17101..17256)
                     /locus_tag="ACG80L_001499"
     CDS             complement(17101..17256)
                     /locus_tag="ACG80L_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS5023736.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         5627 a         3103 c         3767 g         4779 t
ORIGIN      
        1 cccacgctcc ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat
       61 agattcatca aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg
      121 ctcagcttcg ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag
      181 tttatctact gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt
      241 atcgaatatt tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg
      301 gtgcatcacg ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg
      361 ctgcaattgc ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg
      421 tcggcaatac ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga
      481 accataaagg attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat
      541 gttaaaacac ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta
      601 gactgtttgt ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata
      661 aataaaagaa aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat
      721 gaacaaggat gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca
      781 tatatttctt atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc
      841 atggctgaat tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca
      901 gtaaaagtag tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg
      961 aaaactggtg cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg
     1021 aatatgtaca agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca
     1081 gtgacgacaa atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa
     1141 acaggatttg ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt
     1201 gcccatcttg cttctattga aggagaagtc ccttttttga atttctttga tggattccgt
     1261 acgagtcacg agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc
     1321 aatcaggaga aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt
     1381 agtggtacga accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat
     1441 tatgataaat tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga
     1501 acttcttatg acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct
     1561 atgggatcag ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg
     1621 aaaacaggtt ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa
     1681 aagttgcctc atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca
     1741 ggtggagaac ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg
     1801 gctgttattg gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca
     1861 gcagtttttg atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg
     1921 atcgtggatg atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa
     1981 ccgcaaacat tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat
     2041 aaatcagcta tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat
     2101 tatgattcga aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg
     2161 atacgttcag cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac
     2221 ctgcatagct atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact
     2281 ctttggagcg acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct
     2341 gaaaataaca ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg
     2401 ggacgccgga tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct
     2461 tttgatgagg tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca
     2521 atggctatcg tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa
     2581 gtcgcgatcc cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac
     2641 actggttata tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct
     2701 gttggtacac ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct
     2761 atggaaaagc ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg
     2821 tgtaatgaat gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa
     2881 gaggaaatgg aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc
     2941 gtaaaatacc gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag
     3001 gcttgtcccg caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa
     3061 gctgtgaatt gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag
     3121 aatactgtct tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca
     3181 ggctgtggcg aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg
     3241 atcgcgaatg cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat
     3301 acgaccaatg aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct
     3361 gaatttggtt atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca
     3421 atcaaagaag tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta
     3481 gtacacatgt ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc
     3541 gaaagcgaac gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc
     3601 gtaaaaaaca gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc
     3661 gggatcgatc atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa
     3721 gtctattcca atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa
     3781 ttttcggcta gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat
     3841 ggaaatgttt atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca
     3901 tttgaagaag cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc
     3961 acacatggct tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct
     4021 tctggttatt ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca
     4081 atgactttgg actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa
     4141 gttcgctttt cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag
     4201 actgtccacg acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa
     4261 aaaatcaaga aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa
     4321 gaaaaaacga cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca
     4381 gaacgacgta aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa
     4441 tataatgaat aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata
     4501 cttgcaattt tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc
     4561 taaaattaaa acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta
     4621 tgtcatttca aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg
     4681 acttgttctc tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg
     4741 tatacaaact gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag
     4801 ctatttttat tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc
     4861 tgatgaacca agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa
     4921 tccggagagg actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac
     4981 agcaggaaga tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc
     5041 gaaaaatcgg tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga
     5101 cagggattgc tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta
     5161 atacaccgct gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg
     5221 cctatcttcc tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg
     5281 cagctgtcgg tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg
     5341 gggacgtcag catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt
     5401 tggcaatctt gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga
     5461 ttcaatcttt cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa
     5521 acggacaagc attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa
     5581 tgcaaatggc cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt
     5641 tacgggggtg ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga
     5701 aaatgcagtg actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa
     5761 cgaagataca cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca
     5821 tgaagttccg ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc
     5881 gactattaca gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat
     5941 tccttcaacg ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa
     6001 agtctctatc actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt
     6061 ggacgcttcg atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa
     6121 tgcagcaggg gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga
     6181 ttcgatcaag ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc
     6241 cggggtagaa agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag
     6301 cgatttgctt gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa
     6361 tcatcgaaca actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga
     6421 atcagttagt gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac
     6481 gagcagtcgg acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg
     6541 aacggaacca tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac
     6601 cacaagtaca gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc
     6661 ctcagaaaat taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac
     6721 ggcgtacgcg gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg
     6781 tgcggaggtt atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta
     6841 gggcgggata caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta
     6901 tcggtgggta tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg
     6961 acacgtttgc aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa
     7021 gataatggaa tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta
     7081 gaaattgaag cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga
     7141 ttgggaactg tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa
     7201 acgatcaacg gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact
     7261 tcaacaagtg ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca
     7321 aatccagacg ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct
     7381 gagatggtcg ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt
     7441 attattgcgg tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt
     7501 gctaaatact tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg
     7561 agtaacttag gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa
     7621 gttggtgatc gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag
     7681 cagtctgggc acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga
     7741 attcagttac tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag
     7801 gtaacaatct atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg
     7861 gatgttccgg caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga
     7921 agaatcctag ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct
     7981 actgatgaga aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc
     8041 ggactggatt aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc
     8101 tgacactggt ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt
     8161 ttgtcaacgt cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg
     8221 actgtggtta gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc
     8281 ttttctaatc cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc
     8341 aggtttcctc gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt
     8401 ccttcttcga tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc
     8461 tggatataac tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca
     8521 ccacgtccaa tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct
     8581 aaataatctt tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga
     8641 gtgatatcga atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat
     8701 aaagttagta cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt
     8761 ccagcatgca atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt
     8821 gtaatcgtaa gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct
     8881 gctgtttcgc cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg
     8941 ttatagccaa attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa
     9001 gtctgtttac ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga
     9061 tcgctatcag acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt
     9121 acgtattttt gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata
     9181 taaaaaggat aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa
     9241 cgttctacga tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc
     9301 atctcttcat cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg
     9361 ctggcaatca cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg
     9421 acgtaaataa catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg
     9481 acccgcaaag cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt
     9541 agatgagcga taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca
     9601 attttaagtt cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt
     9661 tgggaagtat gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta
     9721 tgaccaatac ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt
     9781 ctgccttgtg ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa
     9841 tcataaccac ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt
     9901 aatccaatca ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg
     9961 tatgaatgct tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg
    10021 gattttctgt agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc
    10081 tcgtcatctg tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg
    10141 ttttttatct ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa
    10201 agaataaaaa ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa
    10261 aaataatggg tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg
    10321 atagaggagg agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa
    10381 aacaattgtc caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag
    10441 atactggctg ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc
    10501 gtgagtttac ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag
    10561 aaaaagcaga gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact
    10621 tgtattagaa aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag
    10681 gattatttgt ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa
    10741 ttgagggatt gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaag
    10801 agggagacag tacgcaaaaa atcgtaaaat tatcagtaga tggaacgatt gctgcgggag
    10861 ggtcttcagg tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga
    10921 aggcgattga agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg
    10981 gcggtgtcta cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg
    11041 atatcccaat gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg
    11101 ctcaagcaga taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca
    11161 tgtcaggatt gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta
    11221 aaagcggagc gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag
    11281 cggtgcttca agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg
    11341 gacggaataa atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg
    11401 tccaagcaaa agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag
    11461 ctatacgaaa ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat
    11521 ctgggtttgc tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt
    11581 ctgagactag tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag
    11641 caatgtatta ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca
    11701 agaaaaagat aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt
    11761 actgagctct tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa
    11821 atggaaaaag tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta
    11881 tttctatgca ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg
    11941 agctattacg cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga
    12001 ttcttatctg agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt
    12061 attgacgaaa ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat
    12121 cagctttgat gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg
    12181 gttcattcta aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa
    12241 aaagcaacat ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa
    12301 agcgttcaac caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca
    12361 ccttctaaaa gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg
    12421 tcatgctttt tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca
    12481 ctgcgtgaag ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca
    12541 agcactagca aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt
    12601 gaaagaaagc tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa
    12661 aaaagaagca ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt
    12721 tgctgaattt tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt
    12781 gatagcaagt acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt
    12841 tgcagaagaa ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc
    12901 ggaggaactt ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca
    12961 gcttccttat catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca
    13021 tattttagca agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcctgt
    13081 tttctatttg acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc
    13141 gcagcagcag gcaagttacc agcaattatg tgtattttca gatcgttacg ggaatgaaga
    13201 tgcgctgctt agttccgtgt taaaagaaga attcacgttg caatctgcaa ttgtttatcc
    13261 tgtctatgat acatacatcg aagacccgaa gcgctggttt caactgactg tagaggcgtg
    13321 tacaggaaaa acgatgtatg aaggtacaga agatgaaaaa caagattttt taaaaaaaag
    13381 agaaaaaatt ttgcagcaaa tgatcttctt ccattagatt ttatcagtga aaaagataaa
    13441 aatgttatga aaaacaagga ttctatccat tcaatatgtt tacaatttga aagggttagt
    13501 gtactattta aaggtatgca tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg
    13561 agggaattaa atgactgcaa catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt
    13621 tacgattgcg caagacgaaa tccaaaaagg attaacaact gcgtttaata aagtgaaaaa
    13681 aagcttgaac gtacctggat tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat
    13741 gtacggtgaa gaagctttat atgaagatgc attgaatgct gtattaccag aagcttatga
    13801 agctgctgtc aaagaagctg gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag
    13861 catggaaaaa ggtcaagact gggtaatcac tgctgaagta acagtgaaac cagaagtaaa
    13921 attaggacaa tataaagatc tagttgttga aaaacaagac cgtgaagtaa cagatgctga
    13981 cgtggatgca cgtatcaaac gtgaacaaga agcacaagct gaattagtta tcaaagaaga
    14041 tgctgcagct gaaaatggcg atacagtagt aatcgacttc gaaggctttg tagatggcga
    14101 agcgtttgat ggcggaaaag gcgaaaatta ctcacttgaa ttaggttcag gctcattcat
    14161 tccaggtttt gaagatcaat tagttggtca taaatcaggt gaaagtctag atgtaaatgt
    14221 aactttccca gaagattatc aagcagaaga tcttgctgga aaagaagcag tcttcaaagt
    14281 gacaatccat gaagtaaaag caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga
    14341 tgttgatgac gaagtatcaa cattagacga attgaaagaa aaatatcgca aagaattagt
    14401 agaaacaaaa gaaaaagcag cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt
    14461 tgaaaacgca gaaatcgttg aattgccaca cgtgatggtt catgatgaag tacatcgttc
    14521 aatggacgaa ttcttgaaca acatgcaacg tcaaggtatc tctcctgaaa tgtactatca
    14581 attaactggt tctacagaag aagacttgca taaacaattc gaaggtgaag cagaaatgcg
    14641 cacaagaaca aatcttgtga tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga
    14701 agaagaaatc aatgcagaaa tccaagaatt atcagaaaca tacaacatgc caatcgaaca
    14761 aatcaaacgc gtgttgacag aagatatgct ggaacatgat gtgacaatga aaaaagcaat
    14821 cgaattgatc acaggtacag cgacagaagc gaaagaatag aaatcaagta ataaatcatt
    14881 tctgaagaag ctacttctag aaaattgttt attcgaaata agggactgtg acacgatttt
    14941 gtcacagttc cttatttttt gttaagagta aaaaatgcaa aggtataggt cagttgatcc
    15001 tttgttaaac actttttctt tttactaaac tatgttatga tagtaaaaga ctgtaaagtc
    15061 tttgatagtt tcttgcatac tttttgcaag ttaaggggtg aagaccatgt atgacaatcc
    15121 aagtagcaat gagaccgtac gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa
    15181 gatcgtagct ggtccagggg tctacatctg taatgaatgt atcgacctat gtaaagaaat
    15241 cattgatgaa gaattttatg aagaagctgt acgcgaattc actgaagtcc ctaaaccact
    15301 agaaattctt gaagtattta ataactacgt gattggacaa gatcgcgcaa aacgctcgtt
    15361 agctgttgcc gtatacaatc actataaacg tgtcaatcag caagcacaag aaaacacaga
    15421 tgatgttgaa ctgcaaaaaa gcaatatctg cttgatcgga cctacagggt ctggtaaaac
    15481 atttttagca caaacattag caaaaacatt gaatgtacct tttgcaatcg cagatgcgac
    15541 aagtttgaca gaagctggtt atgtggggga agatgtagaa aacattttgc ttaaattgct
    15601 tcaatctgct gattacaatg tggaacttgc agaaaaaggg atcatctata ttgatgagat
    15661 cgataaaatt gcgcgtaaaa gcgaaaatgt atctattact cgtgacgttt ccggcgaagg
    15721 tgtccaacaa gccttgctga aaattctaga aggaactgtt gcaagtgtgc cacctcaggg
    15781 cggacgtaaa catccacatc aagaatttat tcagattgat acgacgaaca tattgttcat
    15841 cgtaggtggt gcatttgatg gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac
    15901 gattggattt ggtacacaaa accaaaaact ttctgaagac gaaagtgtca tgcagcatat
    15961 cattcctgaa gatttactaa aattcggatt gatccctgag tttatcggac gattacctgt
    16021 tatggcagct ttagagaaat tgacaacaga tgatctggta cggatcttga ctgagccgaa
    16081 aaatgcgcta gtcaaacaat atcaaaaact tctttctcta gataataccg aacttgaatt
    16141 cgaacctgaa gcgttgagag ccattgcgaa aaaagcaatc gaacgcaata ccggtgctcg
    16201 aggacttcgt tccatcattg aagaaatcat gatggatgtg atgtttgata tcccttcaaa
    16261 tgaaaacatc gaaaaagtga ttatcacaaa agaagcagct gaattgtctg gtgagccaac
    16321 agtaatctat aaagattcag agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt
    16381 cattttggct tttgcgacag ccgcttgctt tattcatcac cttgttaagg agagagacca
    16441 tgaaagtcca tcaagctgaa attatcataa gtgcagtggc accaaaacaa tatccagata
    16501 cacaattgcc agaaatagct ctggcaggac gatcaaatgt agggaaatct tcttttatca
    16561 acacattgat tgatcgaaag aatttagccc gtacttccgg taagccagga aagacccaga
    16621 cattgaattt ttatttgatc gagaacgcat tacattttgt cgatgttccg ggatatgggt
    16681 atgcaaaagt atccaaaact gagcgagcaa aatggggaaa gatgatcgag acatatttaa
    16741 cgactagaga ccagctacgt gcggtcgttt ctttagtaga tttgcgtcat aagccttctc
    16801 aagaagatat ccagatgtat caatttttga agtattatga tatccctgta attgttgttg
    16861 ccactaaggc agataagatc cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa
    16921 aagcgttgga ttttgatcgc aatgacacgt ttattctctt ttcttccgtt acaaaacagg
    16981 gaaaagaaga agcgtgggca gcaatcgaac aagcaattga atcataaaaa gaataaaaaa
    17041 gacttaccag tcaaactctt tcataaaagt ttggctggta agtctttttt tgagcttaac
    17101 ttacttttgt ttcttgtctt ttttcttttc attttgtggc tgtggtttta gaaatcgatc
    17161 gatctcttct tttggtgcaa ctgttttttc cggatcgaca gcaaatgatt cgaaaagttt
    17221 atctaaatta tctgtacgtt caaatttcaa ccccattgta aacacctctt tgtttc
//
LOCUS       DAZUMB010000051        17249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000051 DAZUMB010000000
VERSION     DAZUMB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.051"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..393
                     /locus_tag="ACG80L_001500"
     CDS             <1..393
                     /locus_tag="ACG80L_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023737.1"
                     /translation="VYGFVFLTNKKAGSTMDHLYIKYIHQFSVLVLGTLIIAGIVAFI
                     IQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQ
                     CLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            488..1372
                     /locus_tag="ACG80L_001501"
     CDS             488..1372
                     /locus_tag="ACG80L_001501"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5023738.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1596..4763
                     /locus_tag="ACG80L_001502"
     CDS             1596..4763
                     /locus_tag="ACG80L_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS5023739.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4831..5328
                     /locus_tag="ACG80L_001503"
     CDS             4831..5328
                     /locus_tag="ACG80L_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS5023740.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5347..5811
                     /locus_tag="ACG80L_001504"
                     /pseudo
     CDS             5347..5811
                     /locus_tag="ACG80L_001504"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5808..6566
                     /locus_tag="ACG80L_001505"
     CDS             5808..6566
                     /locus_tag="ACG80L_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5023741.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6652..7392)
                     /locus_tag="ACG80L_001506"
     CDS             complement(6652..7392)
                     /locus_tag="ACG80L_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5023742.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7453..8706
                     /locus_tag="ACG80L_001507"
     CDS             7453..8706
                     /locus_tag="ACG80L_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5023743.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9028..9255
                     /locus_tag="ACG80L_001508"
     CDS             9028..9255
                     /locus_tag="ACG80L_001508"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5023744.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9378..9704
                     /locus_tag="ACG80L_001509"
     CDS             9378..9704
                     /locus_tag="ACG80L_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5023745.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9772..10485
                     /locus_tag="ACG80L_001510"
     CDS             9772..10485
                     /locus_tag="ACG80L_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5023746.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10795..11646
                     /gene="licT"
                     /locus_tag="ACG80L_001511"
     CDS             10795..11646
                     /gene="licT"
                     /locus_tag="ACG80L_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5023747.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11767..13632
                     /locus_tag="ACG80L_001512"
     CDS             11767..13632
                     /locus_tag="ACG80L_001512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5023748.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13646..15089
                     /locus_tag="ACG80L_001513"
                     /pseudo
     CDS             13646..15089
                     /locus_tag="ACG80L_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15197..15898
                     /locus_tag="ACG80L_001514"
     CDS             15197..15898
                     /locus_tag="ACG80L_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5023749.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16024..16536
                     /locus_tag="ACG80L_001515"
                     /pseudo
     CDS             16024..16536
                     /locus_tag="ACG80L_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(16934..17143)
                     /locus_tag="ACG80L_001516"
     CDS             complement(16934..17143)
                     /locus_tag="ACG80L_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS5023750.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5757 a         2900 c         3710 g         4882 t
ORIGIN      
        1 gtatacggat ttgtttttct cacaaataaa aaggcaggaa gtacaatgga tcatttgtat
       61 ataaaatata ttcatcaatt ttccgttttg gtgttaggca cactaattat agcggggatt
      121 gtcgcattta ttattcaagg ccgtgacata ttaacagtac atcaagagtt tttcagttat
      181 gcatttggta ttcctctgat cgctataagg tctgataaca aaaagtggaa gaaacgctat
      241 tacattctct tgctattttt gtgtttttat ctgccaccta agatttttac tttattaggt
      301 atacaatgcc tatcattttc ttttatttcc tatatcattg ttttaatttt cctcagtttc
      361 atagtatgga tgttttatct tgtcaaaaaa taaatgaatg tttcatgaac agtacttcat
      421 cacagcctaa tttttttctt tatgctatcg ttatattaaa gaaatcaatc agaagggaaa
      481 gaaagtgatg aaaaaagttt tattagtggt aaaccccagt tcaggcggtg aacaagcaaa
      541 ggaatttgag caattagcga tagcaaaatt ggaatcggtg tttgatgaag tggtcgtctt
      601 gcatacaaaa aaagcagggg atgcaaaaaa ctttactcgc gaagcggcta cggaagggta
      661 tcatagtgtg tttgtcatgg gcggagatgg aacagtcaac gaaggaatca gtggaatcgc
      721 tgaacaagaa catcggccaa atttcggatt tttcccattg ggtacggtga atgacttggc
      781 tcgagcatta gggataccgc tagaacctga ggaagcgatc aatcattttt cgattgaatc
      841 agtcaagcca ttggatattg gaaaaataaa tgacgactat tttatgaatg tcgtcgcaat
      901 tggctctatc cctgaatcaa tcaatgatgt agatgcggag aagaagacga aatttggaaa
      961 actagcttat ttcatgtcag gaatcaaaca gttagcttcc acacaatcct actcgtttca
     1021 cgtagaagtt gatggaaaga aggaagaaat tgagagcagt accttgctga taggactaac
     1081 taactcagtc ggcggttttg aaacattact tccaaatgca aaagtggatg atggtaagct
     1141 ccatttggtt tatttaaaag attcttcttt gatcgatacg cttaaaacat tgccagacct
     1201 attaaaagga gtagatgaat ctacgaaaaa tctagcctac cttacttgtg agcaattagt
     1261 tgtgtcatta gctgatgagg aagaattgac gacgaatgtt gatggagatg aaggggataa
     1321 attacctgtg acaatcaaag tattgccctc acatttacag gtttattgtt aaaatggagg
     1381 ttgtgaccga ggagttggcg ggagaacacc gcttgttcgg gataagggac tgtgacaaga
     1441 cttttgtcac agcctttttt ttgtacaaaa aagaaattta cacctaatag aaaaattttt
     1501 ccataaaaat acatatttta aatatgaaag tacaattgga aatagattaa aacgataaaa
     1561 taaaattgtc aaaacgtcag agaggatggt ggagaatgaa tcaatgtgta aaaaagctga
     1621 ttgcgtcgat tttgtttgtc ttccctctct tgatggtgac atccgttttt gccgatgtag
     1681 agaaaacaaa cgaatcaacg caatcagaga atcaaagttt tgacaagact actttttcag
     1741 aagaatcgat tacttctgaa actacagaat ctcaaagtga ggcttctact aataaaaggc
     1801 aagaagaaaa taagacaagt gagacctcag aagtagctga acaaacgtct gatagcagtg
     1861 aagcggcact ggcaagagct gtttcagcgg atgggtacag cgaagctgcc acaacagaag
     1921 aactggcgca gcttctcgca gacgaatcag taactaagat ccgactgatt cagcctttga
     1981 ctttagacag ggaattagag atcaaacgag atatcgttat tgattttggt ggattcgctc
     2041 acaatttcgg cacacaccat atttatatca atgagatacc tcagtcgatc gagttccaga
     2101 attttaaagg aacagctgca catccaggtg gattgacacc aaatatcgat ggaaatgcca
     2161 ttatcattgc ttatatgacc gatttttggg gaagcagata tcattttacg ggagaaataa
     2221 aatttaccgg gacacttgat ttgtatgatg ggtccaaatt aggactgatt tacgctcctc
     2281 gtgcaactgt cacattagat ggtgtttccg gtgtgttgga tgtccaacca gtgaaggaag
     2341 gactgaatgc cgctccgggt aaagcctatt tttgtagaag ctatcaattg aatgtgatca
     2401 atggtagtca actttatgga ccgtatctag ggaaattcta cggttttcta gattcaggag
     2461 atgagactgg tagtggaaaa acagcgccag gaatccatat cttaagcggt tcaaaggtct
     2521 ctcttgatta tgatcgggca gatggcgtta gcgatgaagg agaggcagtc gatacgctac
     2581 ctggtaatgt catgtttgaa gtcagtggtt cgggaagcga attttcggta aatacaaaga
     2641 tcaatattac caatgacaac aatcgcggaa tcatccaaat gagaggttca ggatcgaaag
     2701 tcactgtatc gaatggtggg aaaatcacca tcaatacagc aactacgggc ggatttcgct
     2761 tgcaaggaga ttctagtcaa atccatgtat caagtggtgg acaaatctcg gtcaaaatgg
     2821 ctggagatga cgatcaaccc gggaacaatg ggatgcgttt tgtaggaaaa ggcctttctt
     2881 tgatcgtaga aggagccgga tcagttatcg acatcgataa gcagagtggg cgttccagcg
     2941 cagttcgttt tgagaatggt acgcaaaagc tgacagtaac caatggaggc tcattgaggg
     3001 taagaaatac aggtgatggc aaatcttacg tgaacagact caatcaagga aatcaagcga
     3061 tccaatttta tgacgcttct ggttttctag aaagtccagg ttcagctgat tttacagtca
     3121 ctggagaaaa atcaaatatc gatatacgcg cagattccgg agcaactgta gatacaactg
     3181 gagatattga tttaaatttt acggcaggtg aaaaaactta tttagtcatg gtcgggaaaa
     3241 caggggaaga taagaccggc atcatcagtg gggatgtgtt gaatgtggac ttagaatctc
     3301 ctacctattt tgatttccaa aacaaacgga ttggaagcga gaccaatggc ataggcggct
     3361 gggtattcca aggaaatgga gattcttctt taaagatcgc ccattcagaa atcgctttat
     3421 ggcgaaaaac aggcgcaaca ttcgataact ttgaaaaaga cccggattat ttttctgata
     3481 tgacagactt atcgatcaat gggaccgact taggcaattt cgtatcaagc cctagtgcag
     3541 gtttaacaaa tgaatttgct cagacaggtg ccggaatgaa aaattataat cgaatcagtg
     3601 ccaataatca gctcccgatg attgaatcct taagagtgcc aacgaatgca gataaaaaaa
     3661 tctatgggca tgtcgttgtt cccgaaggtg tcgaggcaaa gccaagagat gcttggacag
     3721 gcgaagtaga agtgacttta cgtattactt acgcagatac gacgaaatca tctgtagaaa
     3781 ttactgctct cacgcaagga caaacagatg aaaatgaagg ggcaggatat aacccatggg
     3841 gagaaggcga acaaggcggc ttgttcgaag ttcaagtacc caatgatgag agattaaaaa
     3901 cggaggatca agtgcaagtt gttgctgcta aaaaagtaag aggtggccaa gaagtgacag
     3961 aactggactc tgccaaaacg acagtcgatg tgattcctcc tgaacctgct aatttaagta
     4021 ccacattgat caatgcagct tctcgcacga tcacaggtac aggtgaagca ggagccttgg
     4081 tttcattgaa aaaaaatcaa gctgttttgt cagaaacaac tattgatgat acaggggaat
     4141 ttgttctttc tatcccagaa aatcaacttt ttgcaggaga tgtattagaa attgtattaa
     4201 acgaccaagc aggagaagct actgctaagg gtgtgcagaa caagccaagt accaacgatg
     4261 aaattgggaa tcacaatcca agtgatacgc cagttgttta tcatgatgcc ccgccttttg
     4321 cacctgctac gaagatcgta gtagaaggtg gtctctcatt tcttgcccct tccaaactcg
     4381 attttggtca gatcaaagca actggtttga cacaagaagc atttggccaa atggatatac
     4441 aggaaaaatt atggatttat gatcagcgag aaacgaaaaa tccgtggatc ttaacgataa
     4501 aattatctca gcctttcaca aatgatgcag gattttctat gcaggatgtc ctatatttca
     4561 agaaaggaga ggagtatcaa ttagtgaatg aagaagaact ggaaattgtc agaggacaaa
     4621 attcaacgaa tgtgcaagca gattattcag aatatttaaa taacggtcgg gcgtttaaac
     4681 ttgtcttaac caaagaaaat cagatagctg gtaattactc agcagagata acatggacat
     4741 tagcagatgc gccgacagaa taaagatcaa ggaggatgat tgatgaatcg aatatggaat
     4801 aaaagtcatc gaaacaactg ctctttactt atgatccttc tccttggatt catcctttgg
     4861 cagacacctg tccatgcctc cgaattcaat tttgcggtag attttgaatt accggctaat
     4921 cagattgata agaacaaaac atatataaat gtaaggatgg agcctgggaa aacacaggaa
     4981 atcccctaca cggtctttaa ccgaacagat aaatcagtaa cagttgagac attgatccac
     5041 tccgctacga cgaattcaaa cggtgtgatc gaatatggag aatcaaaaag taaatttgat
     5101 gaaacattaa gttatgatat gaaaaactta gcaaaaacag tgggagaaat aactgttcct
     5161 cctcaaagtg cggtcactga gacactttca ctgaaagcgc cagaaaaaga gctggctggt
     5221 gtggtcgcag gagggatcac gtttcaagaa aaaaaagaga acgtttctaa caaaagtaca
     5281 gttggtgtcg tcaatcagtt ttctcaagtg aaagcaatct tgatttgaaa tagcgaaaaa
     5341 gagtcttccc cagatttgaa attaaaaaaa gcttatgcgg atcaaagcga tggaagaaat
     5401 gtgattaagg cgaatcttca aaatccaaat gcttcttata tgttcggctt gactacatcg
     5461 gttgaaatct acaaaaaaga aacaaaagag ccttaccttt caataaaaaa agaaaaaata
     5521 gatgtagcac ccaatacaaa ttttgattta cttgtcccat tagaaggaaa aaaattagct
     5581 ccgggacatt atcaagcggt tgtgaagggt gcttggaaag aaaaacgctg gaatctccat
     5641 accgattttg aaataactga tgatgcagcg gaccaattga atgaaaagga tattgattta
     5701 aaaaatcagc cagaaacatt tgactggcga tggctgatac ttgttggagt cattcttatt
     5761 ggcttatttg ctggattatt tattttaaaa gaaagaagga aaaagaaatg aaaaaaacaa
     5821 ttgcattaag taccgttcta gctggaagtc tattattatg tacacaaagt gtttttgctg
     5881 agaataagag ctcagccact acagaaggaa cgatttctgt gaccaaacca gatgctgcaa
     5941 atcctgaaga cgttgttcca gttcctcctg ttgatccaaa tgatcctgat actcctttta
     6001 cacctgacga accaaatcag ggaacatccg gattactgac gattgaccaa gctccagatc
     6061 ttgattttgg aacaatcaag ttaggtacag caaaaacagt atatgctgca ttacaaagcg
     6121 gaacggacag tactggagca caaacacaag tacagaattt cgtacaagta acggataaaa
     6181 gcggaaatta tgccggttgg aaattatcag tcaaacgaac agaatttatt ggtacaacat
     6241 ctgatacgaa taaactaact ggttcaaagg tgaaattcaa aaacgccgtg attcgaaata
     6301 gcgcaactaa tagcagtgga atgccatcta ccgtttctgg aactggcgct gaaggaatcg
     6361 aaatcccagc agatcaggca gtagatctag taactgcagg agcaaatgaa gggattggca
     6421 catggattta ttcattagga gagaatgtgg aacaagggag acaatcagtg gaattatttg
     6481 ttcctgtatc caattatgct gtagacaact acgtttcaac attcacatgg tcgttaacag
     6541 atgctcctac agaagaggaa gcgtaaaatc atatcaacga tttcagggag tggagccgat
     6601 tcaaggccca ctccctgaaa tctgctatgc ttacgtctca gctcttattc atcaactcga
     6661 agcagttttt tggaagtcat ggaaacgttc ctttgtcttt tcaaagatct ttcgaccaac
     6721 gagtaaggag tcaccgttga aaaattctaa cacataatga gcagaatcaa ttttctgcaa
     6781 ttgcgataag ttgatacagt aagattttaa aagtaaaaag taagcatact tttctaactc
     6841 tgtcttcaat tgttttaaag aagtattcac taatttatct tctgtagttg ttttgaccat
     6901 aacttggttt ttcaaacctt tgatactttc aatatataaa atttcccgag gatacaggac
     6961 gagacttcca cctttttgat caataaagat tttatcgaca tttacgatct gctgttcttt
     7021 ccaattgatc tgttctagca cttgacgaat ttgttcagat aaattcctgt cttttctgat
     7081 atatccaatc ggaaggattt tccgattgat gatctctact gcaaaattcg tataagcggt
     7141 gaaaaaaaca atattactat gtgcattttt tttacgtatt tgttcggcga catcaatacc
     7201 tgtgagtgat tgcttcaagt cgatatcgat aaagaaaaca tcgtgattat caatagtgcc
     7261 caactggatc aactgattga tttgagagat agaagtgagc tgcacgatat tctcgatatg
     7321 atgattgacc aattctctga tgatccattc gctgtgaaac tggttatctt ctatgataaa
     7381 atactttttc atatgcaggc tcctattaaa aaaatatgtg tacattttga aacaaaacaa
     7441 tggaggaata agatggtcgc aacagtgaca ctttatttga cctttacttt gattttagat
     7501 ttaggtatct atttattctt ttcgaaacaa ctgctagtaa aaaacagctt tggttgtata
     7561 gctttgatgg tggtgacact tttacttcct atccctaaag tgagctttcc ttacctagtt
     7621 ggcttattat tgatgtttct tttttatttt actacgaaaa acctgtttat cagctgtttg
     7681 atcagtaatc ttttttttgc tatcactgga ctcagctttt ttctggtttt tgatctgatg
     7741 gattatttag gaaagagaca tcttggaaca gcaagttttt ttgcactact tatcgcattt
     7801 attttctatc tattagtaaa aagtatagac aaacagacga gatttctcaa cttgcttttt
     7861 acttatccaa ctgaacacta ttttggaggt atttgtattt tattaagctg gggcgtgatt
     7921 tacttggtat tgggaagtgt tcacttccgc tcatctaatt atctgcttct atctcttttg
     7981 tcgatagtca tcagtttttt ttctttgttt ttttccatta tgttgatcag tttccaagta
     8041 aaagaaagac aacatcagga atcgcttcaa ctgtatcaaa taaatgaaga atattaccat
     8101 catctagagg aattcaaaca tgattataaa agtctactgt tttcattaaa aattactctt
     8161 caggaaaata aaaaagaaac gttggaattt ttagaaatat tagaagatca ttctgcagct
     8221 atttttgaaa aaccgcaaat acaacagcta acaaatattg tatccccagc tgtcaggggt
     8281 gtctttttaa aatttctaga aaaagcagaa tcggagaaaa tcccgatcca agtagttatt
     8341 cctgtcgaag tgaaaacaat cccgattgat ctggttgttt ttattcgctg tttatctatt
     8401 tttatttcta acgcattcga tcatcgtgta gccgatcaga gtccgattac gatcaatctg
     8461 ctggaagaaa aagagggatc acgttttagt atagcgaaca aggcaaatcc aacgaatttg
     8521 acgctatcgg agatggtcaa aagaggaaaa acctctaaaa aaaatggcgg gttaggtcta
     8581 tacagtgcga aaaaaatgtt ggatgtttac gaaaatgcag aactaaaaat cgaattttca
     8641 aaacagaatc actatttttt tgtagaaatg tatctggcat ctagaaagag caaatcagcc
     8701 tactgactta ctgtcaaagg ataagtttga ggaaagtcta cacggagata gagacaacaa
     8761 cacagcatat tcaaaagaaa cagaagcgtg atgaccgttg tcacccttct gtttcttttg
     8821 ctatgtagtt aaacaaagta gttgacactt ccgctactta gtattagtac gaaagtatac
     8881 ttcatgatac taagtagata agaagtgaaa agcggaaccc acgatcgtca aaatgctaac
     8941 tgctttgctt acaccagata aagggaagag caatatcgct tattttaggc gtggagaagc
     9001 aagcaaatcc ctacgatcac aaaaaaaatg ctgacccagc agttaagaga gttagaacaa
     9061 gatgaaagta tccatcgaaa agcctataac caagtcccgc ctaaagtaga gtactcgctc
     9121 accgaagaag gaaaatcatt aagagagatt ctagtagcgt tgtctgtttg ggaagaagaa
     9181 cgtatcgaaa aacaaaaagc ctcaagggaa gaagacacgc tactgcatac acatggtggc
     9241 tacctaaatt attgattttc tatgacattt attttaaata aaagaaaaca ggagtttttt
     9301 taaaagcatc atctattttt tatgtaaaaa aatggtatga ttaatcaagt aagaagtgat
     9361 tggaagaagg caagcaaatg aatagtatgg aaaaagtcaa ccagtttaga gatgaacgta
     9421 attggcgaca atttcataac gaaaaagatt tggctatctc tatttctttg gaagcatctg
     9481 aattgttaga actttttcaa tggaaaacgc cggaagaagt aaaagaaaca caattagaac
     9541 gaatcaaaga agaactggca gatgtattga tctattcata catgatggca gacaaccttc
     9601 acctggatct agatgagatc attgaagaaa aattgatcaa aaataatttg aaatatcctg
     9661 tagaaaaaag cgcaggtgta cgaaaaaaat acacagagtt gtagaaatgt gtcattcggc
     9721 tgttggaaag agcaaaagaa aaacagtaaa atgagtctgg agggagagaa aatgaaaaaa
     9781 aaaggaaaag caaaaagatg gctagtcaat atcctcttgt tcctcttgtt attagtagga
     9841 ctagctttga ttttcaatga acagatcaag gattattttg tcagagagac aggagataaa
     9901 tatgctatag cgaatgtaac aaaagaagat ctgaaaaaga ataacgataa agatgtcagt
     9961 tttgactttg atgcggtaga accaatgacg acagaagggg tcatgaggtc gcaaatgaga
    10021 ggaacagatc taccagtgat tgctagtatc gcagtccctt cagtttcaat caatttaccg
    10081 gttttcaaag gcttggataa tacgtcacta ctttacggag caggaacatt atctccagat
    10141 caagaaatgg gaaaaggaaa ttatgcctta gcaagccatc gtgcaacgaa ccctgaattg
    10201 ctgtttacac cgctagaaaa tttagagatg ggtgccaaga tctatttgac agatttagaa
    10261 aatgtgtata catacaaaac attttttaaa gaaaaagttg ctcctacaga tacccaatta
    10321 ctgaatgaag tcgagggaaa agaaattgtc acactaatta cttgtggcga catggacgcc
    10381 gtcacgcgac tagttgtaca aggagaactg gaaagtgtta catcgataaa agatgcgaca
    10441 gatgatatga gatctgcttt caatctagag acaaaaacat tttaggatac tgggacataa
    10501 gtcgttacag attaaaaaca gccaaaggat atgaatcgat aaggaaaaac caatttgctg
    10561 aaaaaaagag aaacgcaaag aggtgcatct ctgatttggt tgttttctag atagacttgt
    10621 gttccagttt ttttattttt tatatgaaaa ggcttgcata aataaaaatt cgatggtatg
    10681 atgattttga ataagaaatg gattgttact gtacggcagg caaaaccaga attcagctaa
    10741 gaaacagtag gcgaattcta ggttttttta tttttcttga gagggcgaag caaaatgaaa
    10801 attgataaaa ttttgaataa caatgtcgtc atttcgaaaa atggattcgg ggaagaagtc
    10861 gtttgtatgg gaaaaggact agcttttcaa aaaaagattg gggacgagat ttcgccagaa
    10921 gcggtccaaa aagaatttgt tttaagagat tcttttgcta aaaaccaatt ccagcagctg
    10981 atggctgata ttccggcaga ggagatcgaa tgggtcaaac aagtagtcga actggctgaa
    11041 gataaactaa aagttgaatt ttcgcctaat atttatctga cattgaccga tcatcttcat
    11101 tatgcgatca cacgagcaaa agaaaatatc caactgccta atccattatt atttgaaaca
    11161 aaaaaatttt atccgaaaga atatcaagca gcgaaagagg ctcttttgtt cgtgaaacaa
    11221 aagacgggtg tggaactttc tgaggatgaa gccggtttta ttgcctttca ttttgtgaat
    11281 agccaacaag gaaacgaaga catgcaagtg acgatgaccg ctaccacaat ggtaagagat
    11341 atactgagta tcatcagtaa attcttcggc atcgtgttcg atgaagaatc tttgaattat
    11401 cagcgtatca ttactcatct gcagtttttt gcccagcgtt atctcaaagg ggaaggatct
    11461 gacgagcaag atgaatttct ctacgcatta gttcaaggaa agtatccaaa agcgtttcat
    11521 tgtgtcgaac ggatcaatga gtatcttcga caaacgcaaa aacaggaaat ggggatcgca
    11581 gagcagattt atttaacgat tcatattcaa agagtagtaa gtgagaaaaa aagcatccaa
    11641 tcataaggat tgttactgta tggcaggcaa aacctggatt gaaaaaatag gttcacgaat
    11701 gtttgtgact gtttttttca atctgggttt ttatttttta aataaaaaag aaagaaggaa
    11761 aaaacaatgg ataatcaagc agttggtcgt cgtgtatggg aagcggtagg aggacaaaag
    11821 aatgtcaaaa gtttagtaca ttgtgctacg cggttgcgat tcagattaaa agatgaatct
    11881 ttagcagata cgcaaaagtt aaaagaagac ccggacgtga tccaagtcgt acaaagtggc
    11941 ggtcagtatc aagtagtgat tggcagtaat gtagcagatg tttatcaatc gatcgtggat
    12001 gaggaaggtc ttgcagtttc ggaggatgga aaagaagagt ccaaaaatat tttgaatcgt
    12061 ctgatcgata ttatctctag tatatttaca ccttttctag gggcgatggc agccgccggg
    12121 gtattgaaag gattcttatc attggcaacg gtgatgggct ggctgacagc tgatagcggt
    12181 acttatcaaa tattgtttgc tgcagcagat ggtgtcttta ccttcttacc tgtcatgctt
    12241 gcttttacag cagctaaaaa gttcaaagcc aaccagtttt tagcagtagc aattgctatg
    12301 gcacttgtgt atccagctat cacagcagtt gctggtgcgg gagaagctat cagtttcttt
    12361 gggatcccag tgattctttc accatctggt tatacttctt ctgtcatacc aatcattcta
    12421 gccgtatggg ttcaaagtaa attagagcca ttcgtaaaaa aagtgattcc gcaatttctg
    12481 caaatgattt tagttccgct tgtcgtttta gtagtgatgg ttccattaac atttcttgct
    12541 ttaggaccaa tcgggacggt tgccggtaat gctttaggcg gtctcttcaa ctcgatttat
    12601 ggatttagtc caatcgtagc aggtctaatc atgggaagtt tatggcaagt attcgtgatg
    12661 ttcggcatgc attggggatt cgtaccgatc atgtttttga atatcgaaca atatgggttt
    12721 gatgtattga tgccaatgct tcttccagcc atcttggcac aaggtggggc tgctttagct
    12781 gtagcattgc gaacaaaaga tacgaaactt cgagcattag ggatttcttc tacagtgact
    12841 tcgttatttg gaatcactga accaactgtg tatggtgtga cattaccttt gaagaaacca
    12901 ttcattgccg cttgtatttc tggaggtatt ggtggagcta ttatcggatt ttccggagta
    12961 aaagctttct ccagcagttt ggtcagctta ttgacgattc cgactttcat taatacggtg
    13021 gatggtgtag agtcaaatgt gacagtagca gtaatagcta caggaattgc ttttgttctt
    13081 gcattcgtag gtacattgat tttaggattt gacgaacaaa cacaagacaa tcaattagaa
    13141 aacaaacatg cgaatgcagg aaaaccgatt acttcagcac ggcatacctt aaaaagtcct
    13201 ttaacgggta aagtactgcc attgagtgaa gtaccagacc aagttttttc ttctggcgtg
    13261 atgggaaaag gtatcgctat tgatccagaa gtaggagaac tagttgcacc tgcagacgga
    13321 gagatcacga ctatttttcc aactggacat gctgtaggga tcacaacgac tgacggagcg
    13381 gagattttga ttcatatcgg gatggataca gtagaattaa atgggaatgg ctttgagatc
    13441 ttagtcaaac aaggggatct cgtcaaagca ggagatttat tgattcgttt tgacatcgaa
    13501 gcaattagag cagctggcta tagcgtaatc actccagttg tcattacgaa tactgatgca
    13561 tttgccgata tacttgaatt agaccaaaaa gagattattg caaacgaaga tgttctggca
    13621 attgtaaaat aaggaggaaa tcaggatgca atcacgtttt tcagaaaaat ttttatgggg
    13681 tggagcaact gctgccaaat caattagaag gggcttatga tcgagacgga aaaggactat
    13741 ccgttgcaga tgctatgcct ggaggtaagc agcgctttgc gattatcggc agtgaagaat
    13801 ttgactggac gattgatcaa gaaaaatata tttacccgaa tcatcgagga atcgatcatt
    13861 acgatcgttt caaagaagat cttgccttgt ttgcggaaat gggattcaaa tgttatcgtt
    13921 tttcgattgc ctggacacga atttttccaa atggagacga aggtactcct aacgaagcgg
    13981 gattagaatt ttatgatcag ctgattgatg agtgcttaaa atatgatatt gaacctgtga
    14041 tcacgatttc tcattacgaa atgcctcttc atttggcaaa agaatacggt ggctggaaaa
    14101 atcgaaaact gatcgacttc tatgaacgat ttgctcagac agtattagag agatacagca
    14161 gtaaagtcaa atattggatg actttcaatg aaatcaattc ggcctttcac tttccagctt
    14221 taagtcaagg attagtaaaa agcaatggag caggagagta ccaaaatatt ttccaagcat
    14281 ggcataatca attcgttgca agtagcaaag ccgtaaaaat cggccacgaa ttgcgttcgg
    14341 atatccaaat cggctgcatg attatctatg caacaactta cagcatcgat gccaatccag
    14401 tcaatcaggc agcaacaatg atccaaaacc aagaattcaa ttttttctgc acggatgtcc
    14461 aagtacgtgg agaataccct gcatacacag cgcgtaccca taaaaaatat ggagtcgatc
    14521 cggaaaaatt agagcaaacg gaaaaagact tccagttgct aaaagaatat ccagtggact
    14581 acatcggatt tagttattat atgtctacgg caatcaatga gactgatcca caagcagcaa
    14641 cttcagaagg gaacttgctg ggaggagtaa aaaatccgtt tcttgaggca agtgaatggg
    14701 gctggcagat cgatccagaa gggctgagaa tcgcattgaa tgaattatac aatcgttacc
    14761 agaaaccatt attcattgtt gaaaacggtc ttggagccat tgatcatatg gaagaggatg
    14821 gcacgattat tgatgattat cggatcgatt atttacgtcg tcatatcgaa gcgatggcta
    14881 atgcagtagc agacggtgta gatttgatgg gctatacgcc ttggggatgt atcgatcttg
    14941 tcagtgcatc aacaggagaa atgagtaaac gttacggctt tatctatgtg gatctagacg
    15001 atgaaggaaa tgggacactg aatcgatcaa agaaaaaatc ttttgattgg tataaagaag
    15061 taattgcctc aaatggcgta aacttgtaat taaagcagct gaatgaaaaa gaacacactt
    15121 ctcacatgtg atttgtgttc tttttttatt ttttatgtgc tattctttta gtaatagtaa
    15181 agcaaagggt gacgttatga aaaggacacg tgtactatac ttggaagtcg cagataaaat
    15241 taaagaagat attttctcag gaaaatatcc agtcggttcg atgttgccga cagaaagtga
    15301 attagaagaa ctgtttcaag tcagcaaaat cactgtccga aaggcaatcg aactgctggc
    15361 atcagatgaa tatgtcgcaa agaaaagtgg tcgtgggacg acggttctaa gcaatcgtcc
    15421 ttacaatcga ttatccaaag cggcaagctt tacgcaaatc ttggagaact cccgatatga
    15481 agtccggaaa gaaacgctgg atttgcaatt ggtccatata aagaaggaag atcctttgtt
    15541 ttcctttttc ggtgagcaag ctgtttgttt taagcgctta tattatttga acaatcagcc
    15601 ttatatttat tttgttcatt atcttccaca agaaatgatg caattcagca gagaagaatt
    15661 tggcaaagaa tctttgtatc gtttgctgac gaaaaaagga tatactattg actcattttc
    15721 tgatgatttc tcggcagtat ttttaacaga agaagaacaa ggaattctga aaacagacgc
    15781 acagatcggc atcaaacgga tacggaaatc gtctacgcaa attggggaaa tgattgaata
    15841 ttcggaggcg gtctaccata cggagctgca tccttaccat attgattatg aaacataaat
    15901 aaaaaaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
    15961 tagcttctca tatttttcgt ttggttaggc ttattaccgc agatacacgg ctgtttctgg
    16021 attaccattt attctggata agggggtgtg gcacgatttt cagaaggaat ctatgaacca
    16081 atcactgtat tgattttagc ttcgatggta accccattcg gcttgacgat cgcttatttc
    16141 ttagggaaaa tcattcgtaa aaatatactc aatcgtcaag aaatcgatac attgaagacc
    16201 gcttttccta tggggatctg tcagatcaca gagggatgtt tccctatcgt attgaacgac
    16261 ttggcacgca atgtcatagc aacgggagtc ggtgggacag taggtggcgg attaagcatg
    16321 ttttggggcg cagatagccg tattccggca tcgggaatgt ttgcagtcgc tacgatgacc
    16381 agaccatggg cattcattgg tgcattgtta gctggttctt ttgtcacagg aataaccatt
    16441 ttattattga aaaaaccagt agatccgaat gcagaaatca tccaagaaga aaaagaggaa
    16501 gaagacatat cttgggatga tttgacaatt agttaggaga ggcagaatgg attacaagaa
    16561 aaaagtacaa tagatgtacc ttgagacagg aatggtatga aagaatcaga gttagaaaga
    16621 atcgaatata cagattttgg tttaggagcg atcgaaactg tccagagcat cggcaccctc
    16681 cttgaaatga cgatgaagga aaaatgagaa cctttcgttg tcgaaagggt ctggtatacc
    16741 ttatgtcgaa ggaaaggaaa caaaaaaact ttcctgtact attccaaaaa ggaaagaaag
    16801 ttattataca gtaaaaaaag aaattattct tcatgctaga gatcagtaca cgttcgagcc
    16861 caatatcaaa cactagttcc aggcaggcgc aacaggtgcg gttatcactg aattttcttc
    16921 cagcagtgac gattcaagtg atatttttac aaatccagct atttatcgtt aacaaaaaga
    16981 tttttaggag aagctgatga acaaaaatga tcaaattttg atcgtttagt tcgtcggctt
    17041 ttttatttag agaaacatca tttatcagtg aaacataaca cgagaacaaa atgatttttt
    17101 cgttaataaa agaaacaagt atttgttgtt taaaataata cacgttattg gcatatagga
    17161 cgaaatccca gtatcttcta taagtaagtc atttcatatc gatgaaaaca cgttatttat
    17221 ttttgtttta tctataaaat ggctgaaaa
//
LOCUS       DAZUMB010000052        16993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000052 DAZUMB010000000
VERSION     DAZUMB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.052"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(504..3209)
                     /locus_tag="ACG80L_001517"
     CDS             complement(504..3209)
                     /locus_tag="ACG80L_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS5023751.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3515..4462)
                     /locus_tag="ACG80L_001518"
     CDS             complement(3515..4462)
                     /locus_tag="ACG80L_001518"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS5023752.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4652..4921
                     /gene="rpsN"
                     /locus_tag="ACG80L_001519"
     CDS             4652..4921
                     /gene="rpsN"
                     /locus_tag="ACG80L_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5023753.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(5097..5459)
                     /locus_tag="ACG80L_001520"
     CDS             complement(5097..5459)
                     /locus_tag="ACG80L_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5023754.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5470..6591)
                     /gene="alr"
                     /locus_tag="ACG80L_001521"
     CDS             complement(5470..6591)
                     /gene="alr"
                     /locus_tag="ACG80L_001521"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS5023755.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6607..6957)
                     /gene="acpS"
                     /locus_tag="ACG80L_001522"
     CDS             complement(6607..6957)
                     /gene="acpS"
                     /locus_tag="ACG80L_001522"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS5023756.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(7087..8598)
                     /locus_tag="ACG80L_001523"
     CDS             complement(7087..8598)
                     /locus_tag="ACG80L_001523"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5023757.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8920..10272)
                     /gene="murF"
                     /locus_tag="ACG80L_001524"
     CDS             complement(8920..10272)
                     /gene="murF"
                     /locus_tag="ACG80L_001524"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS5023758.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10425..11501)
                     /locus_tag="ACG80L_001525"
     CDS             complement(10425..11501)
                     /locus_tag="ACG80L_001525"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS5023759.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11697..13442
                     /locus_tag="ACG80L_001526"
     CDS             11697..13442
                     /locus_tag="ACG80L_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS5023760.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13722..14159)
                     /locus_tag="ACG80L_001527"
     CDS             complement(13722..14159)
                     /locus_tag="ACG80L_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS5023761.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14143..15516)
                     /locus_tag="ACG80L_001528"
     CDS             complement(14143..15516)
                     /locus_tag="ACG80L_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023762.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15838..16326)
                     /locus_tag="ACG80L_001529"
     CDS             complement(15838..16326)
                     /locus_tag="ACG80L_001529"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5023763.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16348..16950)
                     /locus_tag="ACG80L_001530"
     CDS             complement(16348..16950)
                     /locus_tag="ACG80L_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5023764.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4793 a         3627 c         3081 g         5492 t
ORIGIN      
        1 gtcaataaac tttaagaaaa taattggaaa aaaacagaaa aatatcaagc gctttttcaa
       61 acgctttaat acagcaaaaa tctaacattt ttttatgggc tttttctttg aattctataa
      121 taaaaggata gaacttgact tagaaaatca agttctatcc tttttgatat cttaactttt
      181 ggaagagaga tgtaaggctg ctttctaccc atcattctta ctactcgaag catagcgcag
      241 ttgtcacaac ctcttgataa acggtgttta aaagcttgca tctgcggtaa taagcccaac
      301 caaattaaaa acataagaag ctgtttttat ttggtcgttc ttattacttg atgcagagca
      361 gcttttttac aacctcttgg tacacggtgt tcaatcagct gaccctcggt attagctcaa
      421 aaaaacggca aaatatgagg aactattttt ccattttttt ctctaatact caggtcaaaa
      481 cactgatttc acaacctctt tttttaatga aaaatatggc tctcatgcgc cgcttctttg
      541 aatttcgtca ttttttcttc gatgaacgga tagattgccg ttcctacgat tccaaagaca
      601 acaaacaaac cgatcatgat ccagattgct ttcgtagcat ttggccaata aattcctcct
      661 gcagattccc gtaacaagtt taccgcatga gtaaatggca agaacggatt gatcatttgg
      721 aagaatttcc ctgagacttg gatcggatag ttcccgcctc cgccagagat cgacagtact
      781 aagatgataa tagcgatccc tttaccgaca ttatcgaata atgcagccaa gacatagacg
      841 atcatcatga acgctaaggc gatcaagaca gcaaatgaca cgctgtagac cggattttgg
      901 acatctactc ctagtaagaa aatattccct aatgtaacga tcaagctttg cgcaatcgcc
      961 attgtcaaaa atgtcagcat acgagcaacg aattgttctc gtttcgtaaa attgtttcgg
     1021 tctttctttc ctaaatagta ttctgtcgta gcaacacttg aaagaagaac agcgccgacc
     1081 cacagacata gtgcagtata aaacggggtg ctagctgaac cgttatttgc gattggataa
     1141 agcgcattgg tttgtagctc aacaggttgt gtaaagaagt cactttcttt tgttgcgtct
     1201 agtttcaata atttgatgac ttcgcctaga tcgacttctt gttcgccttt tcggatcgcg
     1261 tctgctgctt tttggatacc agctttgata tttggccagt cattgttgat caagtccgca
     1321 gcagcgttca cagctgtttc gacttctggc attttttcat ttactgttgt caatgtaccg
     1381 gtgatttcat tttttagtgc tggccaatca ttttggataa aatcagcggc caatcctaat
     1441 ttttgctgaa tgatcggtaa ttcattgttg tataggtctg ccccttcatt gatgccattg
     1501 acgatggtat ccatatttcc attcagcatt gtattggcat catggatttc ttgtccaatt
     1561 gcaggcattt ctttttgata tttttctaaa attgccactg cattcgttac agttgctttc
     1621 gttgagctga gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc gccttgtgca
     1681 gtcccaatcg tatcgatgac ttggtctagt agagaagtga cagtggaact gatttgacct
     1741 gtattgatac cagcaatgat gccattgata ttattaacaa cggcatcaat ttgatctagc
     1801 aattcatcaa tagccttcgt gtcttcattt tgaacagctg tgttcaaagc agataggcgg
     1861 ttttgcagat tgcttagcaa gggttttaat tgatttagct gatcaataat tggctgtaat
     1921 ttcgtatttc cagcacttgt ttgcaattta gtcaggaact cgaccagttt atcgattgca
     1981 ttttgttgtt gagatattcc gtcaataaag ccgttcaata cattgctgat gtttttcttt
     2041 tcatcatcag tcagcttacc atcttcaata gctgttctta cagtatctat taacgaacct
     2101 gtcgttgttg ccacttgtcc aattgacttc aatgtaacat ctacactgtt tgtgatgctt
     2161 ggtatcgcat ctttcagctg agaggcacct gattttgttt ggtttgctag atcacttgcc
     2221 tgattttcta aatttttcac atctggcaag atatcttgta cttgttggat gatcgtcaag
     2281 ccttgttttg cttcatcgat tccttggttc attgtctctt ggatcgaagc aaaatcgccg
     2341 tcgatctctg ctagttgctt accggcattt tggatctctg ggattttttc ttgcaaatca
     2401 aggatgattt ttgcttgatc tttcaactca ggcattttgt cattcaatgc aacgactttt
     2461 tcacccatct catctacttt agggatgtaa tccacgaatt catttgcttt attcagtttt
     2521 tccttgattt caggtagttg actttgtaat tcgaggactt gttttgtgta gccgtcgatc
     2581 gtgtccaaat tttcatctgt tgataaaatc atatctttaa ctttattgat acttactagg
     2641 ttcgtatcga tgtcgtaacc tatctcattg aatactttca ataaagtcga actggctgtt
     2701 ttgataaatt catttgtgat ttgcgattgg atcgaacttg ctcctttatc ggtgattttc
     2761 ggtgcaatcg cattgatttt ttcatttacc gtatattcga tttttggttt cttgatatca
     2821 ccactagtaa aactcagcag atcttctgag aaatcctttg gcaagtaaat acctgcgtaa
     2881 tactttccgg aacggactcc gtcttctaat tgttcttttg aatcaacgaa ttgccatcct
     2941 aattgtttat ttttatgaag cgattcgatg acttgctccc caatggccac ttctttacct
     3001 tgaaactctg ctggcttgtc tgcactgtaa acagcaattg gcagctctcc agtatttcca
     3061 tatggatccc ataatgcttt gatgttaaac cacgcatata gcgaagggat gatcatgatc
     3121 gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc aagcaaatat
     3181 aatcgaagcg tatttttgat tgactgcata gttctccccc ttcttctttt ttcagctgaa
     3241 ttactttacc atgtatttca tacttttttc aatcggcttt tttcaaaaaa gtcgaatttt
     3301 tggacatatc acggcaattg tctcattgta atctcttaca tattgagaaa taattaagga
     3361 ttacagcctt tactttatca tgaaataaaa gggaaagata attttatgcc aaagttttta
     3421 tctattatac gtatacgcaa aaaagctaaa acattttttg tctttgcccc tacaaaaatg
     3481 tttcagcttt tttcgttttt tattcctgta tttcttattt ttcgaatgac aggatgattt
     3541 tcccagcagg aggatgtcct tcaactagct gatgtgcttg gatgacatgc ttaagtgaag
     3601 cagacagatt cattgaaata aaaacatgaa aggctccttt ttggtaggct ttgattatgc
     3661 cagcaaaggc ttctgcatct agatattcct ttcttggaac aaacgattca taaaagcctt
     3721 cctttttttg ccgcaaatct agatctggga ggtcatttaa tgcaacatac ctgccgcctg
     3781 gtttcatgat ctggatgccg gtttctcctt tgatacttcc tttcgtcgca tcgattacaa
     3841 ggtctgcctg atcagcaaat tgcagacctg gatttgtttt atcataagca gcaaatgctg
     3901 aagctcctaa tttttttacc ttttcctcat ttttactgga tgcactggtt aagattcgga
     3961 ttcccttttc atggagaagc tggatcagac ttgacccgac cgcacctgat gctcctagta
     4021 tcattacagt atctgtcggc tggatttccg tcaaatggtt gattagatta taagccgtaa
     4081 ttcctggcgt gaccattccg gctgcttctt cccaactcat tttatctgga atttttgcta
     4141 cttttgctga tggcaacacg acttcttctc cgtaagtccc gccgactgca tggacagcta
     4201 ctcgatcacc tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc
     4261 catcattacc tggtacataa gggaatttca atgttcgtat ctctttcatt tctcctaaac
     4321 gcaaggcaac atcataagga ttcacactga atgcttttat ttctacacga acatgtccag
     4381 aaccgacttc tctgggatga gcatcgattt cttcaaaaac atcttgtgct tctccgtaac
     4441 cgttaattgc aaatcttcgc atggtcacca tctccctttt tctttaagtt tacgtttatg
     4501 cctttatttt gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa acaaaatagt
     4561 tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat tacgatttac
     4621 gctaataaaa aataattgga ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc
     4681 aaaaaaacag caagaaacga ttgaacgcta tgctgcttta cgccaggaat taaaagcgca
     4741 aaaagattac gaaggcttgc gtaaacttcc tttagatgct cgtccagaac gattgaaaaa
     4801 tcgtgattta atcgatggac gccctcgcgg atacatgcgt aaattcggga tgtcccgtat
     4861 aaaattccgt gaattagcac ataaaggttt gattccagga gtaaaaaaag ccagctggta
     4921 atccaagtct ggctgtaatg taaaggagga gggcgcatgg accactcctt ggatcatcaa
     4981 aaaagatacg ttcgcttctt ctagcagaaa tgctggtact agaagcagac gtatcttttt
     5041 ttacgcacag ctggtccttt tttatatgta aaggctgcat ggttacttcg ctatatttat
     5101 ttagtaacag gagtcagtgc ttcgataccc acactaattt tcaatgctcg gtcaacttct
     5161 gccataatgg gtcgatccaa atggcagacc ttttctttca atcttgattt atcgatcgtt
     5221 ctgatctgct ccaacaggat aacggagtct ctttctattc ctgttccagt agctttgatc
     5281 ccaatgtgag tcggaagctt aggtttagcc atttttgccg ttatggccgc aacaatgatc
     5341 gtcgggctaa aatggttacc tagattattt tgaataacca aaactgggcg tgttccgcct
     5401 tgctccgatc ccacgactgg agaaagatca gcaaaataaa tatctcctct ttttaccata
     5461 ggatcctcct tattcttgat attctcttgg tatccgctgt cctaatccgc aagccacttc
     5521 atagtggatc gtacctaatt gatctgctat atcctgcata gtgatttctt tgtctgcatt
     5581 tttgccgata agcgtcactt ttgttccgac aggaaattct tgaggcaatc ggatcatcag
     5641 ttgatccata catacacgcc caatcgtttc acagtagttc ccttctacca atagagaaaa
     5701 accctgcatc tttcgtggcc agccatctgc ataaccgatc ggtatcgttc cgatccactc
     5761 tgcttcagga gtgatatatg tttctccata gccgattcct tcacctgctg ggagtttttt
     5821 gacttgaatc aattcagaaa ccagctcaag tgctggctgc aatggataaa cttccggtag
     5881 tgcgtgccca gagggattaa gtccgtacat tgctactcca taacgaatca tattgccgat
     5941 tgtttcatca tgccataaag ccgttgcact attgctgaca tgtacatatc gaggaagttc
     6001 gggaagtttt ttcagcactt ctataaagcg ttctttttgc agattccaat atgtatcatc
     6061 tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc cattctaata cacgactttc
     6121 tttgatgaat gcagctgcct cttttacttc ttcaggtgta caaaatccga tacgacccat
     6181 cccagtgtct gcttttagat gaatcgaaag aggcgtctct agcttacttt tctctaaaac
     6241 agcagctgct gcttgcaacc attcccgtgt acctgcagtt actgataaat catgagcaac
     6301 agctacagtc agtgcgtcgt aaggaaccat attcaagata aggatgggct gagtaaatcc
     6361 agcttctctc aactcgactc cttcatctaa atttgaaaca caaaagcccg aagctccacc
     6421 tgcaactgct gcctcagctg tttcaatagc tccatgaccg tatccgttcg cttttactac
     6481 tgcaaacaat tcttttcctt gcggaagtct tgcaacttca tttgctacat tttcagttat
     6541 agctttttta tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc
     6601 ttctttctag tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat gagtaatact
     6661 gacccataca ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat tttcattttt
     6721 taaaatctcg acatcttgaa aggtaacttt tccgatccca gttccccaag ctttagagaa
     6781 agcctctttg caagcgtaac gcccgcctaa aaattctact tggcgatgaa aaggcaagga
     6841 ctggaatagt ttcatttcat cagaggtcaa gattcgtgca atgaattttg gtttttcttc
     6901 gatcagccga gtgatcctag gaagttcgac tgcatcgatc ccaattcctt ttatcatatg
     6961 cttttctctt ttcaataaat ttagaacact ctcattctac caaattataa ccattttgcc
     7021 acaaataagt ccacaaataa caaaaatgaa agtttctctt aaaaaagaga aactttcatt
     7081 ttatgtttaa tcgttatttg aacggattac aaatccgcgt tttttatcat tgtgacgttt
     7141 accgccatct ttttggctgt tattattttt gctataaccg ccttttgatt tgtttccacg
     7201 ataattaccg ccgtcacgac gattacggtt gtttccgcca ccacgtttgc catttttatt
     7261 gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg atcttcactg gcactgcatc
     7321 agctggatct tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg cagaatattc
     7381 ttctaataat ttatctgctg tttgcaggta tttatctaga ccattttctg caagttttgt
     7441 ttcgatttgt tcaactgcag cacctaattg acctttgaat gcttcttttt cggttggtgg
     7501 acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt agtccatttc
     7561 atttggtgta acgaaagtca cagacatacc gccttttccg gcacggcctg tacgtccgat
     7621 acggtgaacg tagctttctg gatcttgagg gatatcatag ttgtacacat gagtgacacc
     7681 tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca aggatatcta aatgaccgct
     7741 cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac aaatcgccat ggattccttc
     7801 tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc gttttgtacg
     7861 tccgaagacg attgttaatt caggtgtttg aacatcaaac aagcgagtca tgatatcgaa
     7921 tttttcgtat tcttttgcgc ggacatagta ttgatcgatc aagtcagctg tcatttcttt
     7981 cgctttgatc ttcacatgat gtggattttt catgaattta acgccaatat tcttgatagc
     8041 tggcggcatc gttgctgaga aaagtaatgt ttgtctttga tcaggaactt gtgaaatgat
     8101 tttttcgata tcttccaaga agcccatatt caacatttca tctgcttcat ccaaaactaa
     8161 tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca tacgtcctgg
     8221 tgtacccaca acgatatgtg gacgatcttt caagccacgg atctgacggc caatatctgc
     8281 tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc cctaaacgat ataattcttc
     8341 ttgtgtttgg atagctaatt cacgtgttgg tgcgataact aaaccttgta attcgtgacg
     8401 atctggatcg attttttcta acattggtaa tccaaatgca gctgttttcc ctgtacctgt
     8461 ctgtgcttgt ccaataacgt cttttccagc taatgctagt ggaatcgttt ctgcttggat
     8521 aggcgttgct tcttcaaaac cagcgcgttc gactgatttc aacaattctg gtgataattc
     8581 tagttcttta aatttcaaat gaatcctcct atatggttgt taactgagga tgtagctttt
     8641 ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca gtggccacgc
     8701 acgtttttat ccattcttag cccctgtggc accaccgaaa aatcactttg cgaagtttag
     8761 gcattagaaa agccaaacgg gtcgcgttca gctttatcat tattatgaca tctggcttca
     8821 acaagttgtt tctatggcaa aaccagtcca atttctcttc atctttcggc aataggttat
     8881 tctaacatag tttttcgttt tctgcaagtt tacagatttt tatttcattt gctgaagttg
     8941 ttctaccact tcaaataagc ccatgctgtt gctgcctttt agcataatac tatctgttgc
     9001 ttcaagattt tgtttgatag catcaatcaa tttattcttt tctttttcaa aatagaacac
     9061 aggtagttgg gggtattttt ggttcaattc ttcttttagt gcatgcattt gttccccata
     9121 aaggaaaatc atggcgaatt tctcaggatc gatatgttca ctcatttgcg catgcatatt
     9181 taaagactct ttccctaatt ccagcatatc tgctaacaca gcgattctgc gtccttttgt
     9241 tggcagattt ttaaaagtat ctaataccag tcccatggct gttggatttg cattgtagac
     9301 atcgctcaaa atagctgcac catttcctgc cgttagccat tcggttctgt tttgagtgat
     9361 agaaacatgg gcgagccctt ggcgtatctc aggtatggac agtccaaaga agcgcccgat
     9421 cgtatacgca atcaaagcat tcgttacatt ataacttcca ggcagcggga tcgtaaatgt
     9481 ttctccttct actacaaaat cggtttgtgc ttttcctttt gctgtcactt ccgcatgaac
     9541 atcgccattt ccaataccga atgttgtgac tgtttgtact aatgttgccg ttaaaggtgt
     9601 cagcaacggt tcatctgcgg gcacaacgag taatccgtct gatacaagtc cgtcagtgat
     9661 ttccattttg gctttagcga ttccttctcg cgacccaaga ttttctatgt gtgcttcacc
     9721 gatcattgtg attgctgcag cttttggttg agccataagt gaaagttccg tgatttcatt
     9781 ggcgtgatcc atgcccattt ccaaaacgat cttttctgta tcttggggca tgtgcaaaat
     9841 cgtgtaagga agaccaatat tattgttgta atttccttgc gtcttatatg ttttgtattt
     9901 ttcagacaag acagacgcgg ttagatcttt cgtggtggtt ttaccattgc ttcctgtaat
     9961 tgcaacgact tctgcgccta ctttttccaa gtaataggtt gccaattgct gcatggcttt
    10021 caaaggatct gctacaggaa taactggaaa attttttgga gcttgttcaa caggcaagct
    10081 ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg ataaattcat gtccatctcg
    10141 cgtaccagca agaggaacga ataaacttcc ttcggttatc ttccggctgt caaattcgac
    10201 attggtaatc tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa tttcttgtat
    10261 tgttagattc attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt ggcctaaacg
    10321 gcgggttcgc atgactctca cttccaagta aatttctcta ttcgtattta gaagctagaa
    10381 tgaacgcggt tacgcaatca ctccagcctc ttctttattt ctctttattc atttttttca
    10441 aaaaaagatt gacgctgatg gtatcgattc attcctaact ggatcagttc ttcaatcaaa
    10501 tcaccgtatt tcaagcccat attttcccat aagagtgggt acatactgaa ctccgtaaat
    10561 cctggcatag agtttaattc attcaggaat aattcatttt tatttgtcaa aaagaaatcg
    10621 caccggctca atccgcttcc acctaacatc gtgtaagcta acttcgcgta ctcttgcgct
    10681 ttttgataaa cttcttctgg cacttcggct ggaatctgca tttcgatttt attattgata
    10741 tattttgctt cataatcata gaatgctacg tcttttacga cttcaccagg caaagtcgtc
    10801 cgaacgtctt catttcctaa tacagcaact tcgatttcgc gcgcttcaat tccttgttca
    10861 acgattgctc gagaatcata ctgataggct gttgctaaag cattttgcag ctcttctcgg
    10921 ttttctgctt ttgtaatgcc gacactagaa cccatattcg ccggtttgac aaacatcgga
    10981 taaagcaaag aaccttcaca ttgatcaaat acttttttag gattttcttt ccattgattc
    11041 ttaagtactg gtacataagg aacttgcggc acaccagcag cttgtaaaat atacttggtc
    11101 atgattttat ccatggcaca tgcactggtc aatacgcctg cgccgacata aggcatattc
    11161 aatgtctcta agaagccttg gatcgttcca tcttccccgt ttggcccatg taaaactgga
    11221 aaaacgatgg ctccttcttc tttgatttcg cccggattga tcacttttcc tgtaaagcct
    11281 tcctctgtct gtccacttgg gtcccatgaa agatgcaaga catctttgct ggcaggtttt
    11341 tctgttagta atggaccttt gacccattgt ccttcttttg taataaatac gagttgaact
    11401 tggtaataat tataataaat ggcatttaaa actgaaaatg cggaaagaat ggacacttca
    11461 tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc ctcctgtatt
    11521 ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa acaaaaataa acgaataact
    11581 tatttataaa aaaacgagcc agcttctaaa agaacgagcg tcatttattt tttgtcagct
    11641 agattatttg tcccgttttt tataaaaagt tatgataaag aaaaggaggg atgatgatgg
    11701 cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg agtgttcatc aattacaatg
    11761 tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc tttgaagaac
    11821 taggccccac cttcatcaag ataggacaga tgctttctgt tcgagaagat ttgctttctt
    11881 ctgccttcac tcaaacattc aaaacattac aagatagtgt acctagcgac acattttcaa
    11941 cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt atcagatata ttcgatgatt
    12001 tttcaaaatc gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc aagctgaaga
    12061 acggtgacgc ggtagtcgtt aagatccaac accctaacat cgccgaagag atccgacttg
    12121 atcttcaact atttgaacga gcgatccctt tgatcaaata cattcctgaa actagcgtag
    12181 ttgatctaaa aggggtactc caagaagtaa aacgttcctt gatcaatgaa atggacttct
    12241 taaaagaaag tcaaaatggc gaacaattct atcaaaagaa caatggctgg aaagagatcc
    12301 gcagccccaa gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg gaagaaatgt
    12361 ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact tttatcactg
    12421 gaattcaaaa caagcagctg aaacaagaag ttgcaaagct tttagttgag aactttatga
    12481 aacaggtgtt tgatgatggt tttttccatg ctgatcctca tccagggaat ctgctattcc
    12541 atgtactaac aaaagaagaa cagacacaag ctagccgaaa aactgaaaca gtccatgaaa
    12601 aagagttcgg ttcctttgct tttcgagcct ccacttctgc agaagaccct gttgcgccat
    12661 ataccatcaa ctacatcgat tttggtatga tggggcatct ttctgcagga cttaggcaaa
    12721 aactcacaca agccgtttta gcgctgtata cgaaagatgc ttatcgaatc gaaaaagcgg
    12781 tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac caagaactga
    12841 caagttttct tgaacagtat tatgattctc ctattgatga aatcaacttg caagaagtat
    12901 tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt gatatcacgc
    12961 ttttgctgaa agcatttggg acattagaag gggtgatccg cgtcctcgat cctgaagttt
    13021 ccctcatgga agtcgcttcg ccttttgctc aacattattt tctcactcat ctggatgtag
    13081 aagatacatt gaaacaaagc ggattagact tattggaagg gatgaaggct gcgcctaaga
    13141 tccctcaaca gctccaccat ctattagaaa tgtggacttc gggacaagga aaagtcaatt
    13201 tggaattgaa aaagcaagac aaattattga gcaggatcga aagtatgatc aatcggcttg
    13261 ttttcggtat gattttagct gcattgatcg tagggtcctc cctgcttgtc caagcagcac
    13321 ctgtagaaaa cgcagaggtc gtttccttat tagggatatt tacgtatgct attgccgcat
    13381 tcgtcattat ttttttggca atcgatgcat tgatccaaat gtataagaag aggaaaaagt
    13441 agaaaaaggt tgtgacagaa agaatcagct tcaagaaata agaaggggtt ttacataaat
    13501 tgtttctcaa atttttgtga aaattcggct tatttttcga agaggctact tctgaaacac
    13561 cgagaataaa aaatgtgaaa aagctgttct gcacccagta ataagaacaa tcaaaaaggg
    13621 aacatggaac acatttatcc tgtttccctg ttcccttttt gatgtattcg agcataaata
    13681 aaataccttc gttacctact atcttctgta cttggtaaac cttagtcaat tgcctttttt
    13741 aacgcttgac gaaaagacat atgatctttc tccgtaaagg gttttggacc ctttgtcctt
    13801 atgccagctt ttcttaactt tccactatga taacgttgtt ctagaagcgt gccgatattc
    13861 gcttcgtcaa attcttttcc agagtgatgg aacactcgca cacccttcgg caatacaagt
    13921 gaagctaaac cataatcttg cgtgatcaaa aagtcattct tcttaaccat accgacgatt
    13981 ttataatccg cactatcagc tccgcggtct acgtagacga tttggacatg attagcgtat
    14041 tctttggtcg tataatggtc cacactggtc acgataacca cgtcaagacc ataggattct
    14101 cctaatttta tgacatcttc ttttactgga gaaccatctc catcaatgaa tagcctcatt
    14161 ctttttcgcc ttcttccata aatgataaac agttactaat ccgctcagta aaagaccaac
    14221 attgatccat tttgccaaga taagcagttg tacactttct atcggcactg attcatggtt
    14281 cagcgtgatc aataatagat ggattggctc tcccatttct tttgctaaaa caaacaaaag
    14341 cgtcatcaat aaaaactggt ggatcacact ccctttattt tgagtagtat acttgacaac
    14401 gatccggcga tccattggaa tagctgtatc agtttgataa tattccttat ttagccagct
    14461 gcctgttact acgtgccaga aactaagcag aaagacacct atcgcaaaac catttggaat
    14521 caagaggacc aataaagaag aactcagtcc aagcaattgc atagccaatg gagaccatgt
    14581 taaacgtttc aaaatccatg gtccgactat ttttgctgca atcaagcctg ctgcataagg
    14641 caaatacata aaaatcgaaa gtttttctaa tccgtacagt atgccaacat aaaaactgct
    14701 aaataaaaaa aggaaattcc ctagcattcc atttagaaaa gtaaacagat ttagccagct
    14761 tggcaacttc cattccttct tctggtgtcc taaaacaaac acgaacaaga tgaacagcag
    14821 caagaaacca taaatagcaa gatcaaaaag cctttcatcg aataacaatc gggcatttct
    14881 caaaagaaac aataagccaa agaatatcaa aaataaaaca agctctcgcg tagctaccag
    14941 atttcttttg atgtctttga aatccagttc gtaacgcgga taatgactaa ccgtatggta
    15001 ggccatgacg taaaaaagtg tataaatagt aaagcttgct gcaatctgag tcggtgcagg
    15061 caactcaatc gcttgataca ggagaaatag ccagatagct gcaaataagt actttttccc
    15121 atccatattg ataaaacctt gcgtcttttc gtgaaaattg accgtggtat tagcaggtac
    15181 aagccatgct gcactcagcc ctagcaaaac acttgaaaaa taaaataaag gaaaataaaa
    15241 tacgcctaat attccaagca atgaaccagc accaccaatc agcagagcga tcatcagcaa
    15301 cgtgtaacta tctagaccaa atcgtagact gttgactaaa aatagtccag tcatccgaaa
    15361 ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg ttagtcccaa
    15421 aaataaaaga taaggaataa aaataaccgt gttacttaag ataaagggcg ttgtttgtgt
    15481 aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca atggctcctt aaacagtctt
    15541 ataactaatc tgctcttcga ttttagcatt aaaggggaaa taaaccaatt ttacagaata
    15601 tattcttgta aaaaaaaata cagaaagaag cataagcttc tttctgcatt tttacggtac
    15661 ttgattactc aagcagacaa gcccttctcc acaaaagtct tgcccgacac ccctgcatcc
    15721 ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc aaaatcaatt aggagctctg
    15781 taaaccagag tccttctcga cccgtcgcat aaatttactt taacacagtt cctatgttta
    15841 tgcgagagct tttttcattt tataatcgac catttgaaat ccttgaccga tatctgaaat
    15901 aagttcttct gtaatctcaa atccttcatg cttataaacc tcgattgctt gatgattccc
    15961 ctgattcact cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt cttcatacca
    16021 gcgaaaaact gaagaagtca atccttgacc acgtacttta tttagcaaat ataatttact
    16081 taaataaatt tttccattta tctcttcata agcagtgtaa ccgacagtct tttcttcgct
    16141 ttttaataaa aagtaggaca caccttctgt cagttctttt tgaatctgtt tttttgactg
    16201 ataagttttc agcatgtaat ccacttgttt ttgaccaatg atcggcacaa aaacttccgg
    16261 ccatacttct tgtatgactg ccatcagctc atccagttca ttttctgtta cttttatttt
    16321 ttccatacac ccacgtcctt ttttacatta taaatgacct tgcgcttgat ggaataaaaa
    16381 gagcatgatc atgctataca cacccaccga ccacggtttc aagaaaatca caatctgcat
    16441 aaattcccta aaagataatt tcgtcaaacc agctactaga cagatcaagt cgtctggcgc
    16501 aaagggaagc agcaaggtaa caactagtac ttttttaata tctttcgttt ttcttgtcag
    16561 caattcatca aatttctgat acttttttgg cgataaaata agctggacaa aggctacgcc
    16621 atagtaacgg acaagcaaaa atccgattgc ttccccaatc actaggccaa tacaactata
    16681 taaaagccca aatccattgc caaacatcag catgcccgct actgaggaga tccctcccgg
    16741 caaaatagga atgatcactt ggatcacttg caacagaaca aatatcatca ctgcatactt
    16801 gccaaattgc tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata
    16861 agcataaaga atcaacacaa agaaaaaaaa cagaccaata atgggcatga tctgaagaca
    16921 tttttgtaaa actgatcctt ttttagacat ctatattccc cacatctttt ttcatttatt
    16981 aaaactgcct cct
//
LOCUS       DAZUMB010000053        16519 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000053 DAZUMB010000000
VERSION     DAZUMB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.053"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..750
                     /locus_tag="ACG80L_001531"
     CDS             <1..750
                     /locus_tag="ACG80L_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5023765.1"
                     /translation="IHYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQVYNQQ
                     EILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENPEN
                     MSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNEYVFTM
                     EDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGHAS
                     YSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1324..1893
                     /locus_tag="ACG80L_001532"
     CDS             1324..1893
                     /locus_tag="ACG80L_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS5023766.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1880..2740
                     /locus_tag="ACG80L_001533"
     CDS             1880..2740
                     /locus_tag="ACG80L_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5023767.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2930..3634
                     /gene="yycF"
                     /locus_tag="ACG80L_001534"
     CDS             2930..3634
                     /gene="yycF"
                     /locus_tag="ACG80L_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS5023768.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3639..5474
                     /gene="walK"
                     /locus_tag="ACG80L_001535"
     CDS             3639..5474
                     /gene="walK"
                     /locus_tag="ACG80L_001535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS5023769.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5471..6787
                     /gene="yycH"
                     /locus_tag="ACG80L_001536"
     CDS             5471..6787
                     /gene="yycH"
                     /locus_tag="ACG80L_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS5023770.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6788..7657
                     /locus_tag="ACG80L_001537"
     CDS             6788..7657
                     /locus_tag="ACG80L_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS5023771.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7712..8521
                     /locus_tag="ACG80L_001538"
     CDS             7712..8521
                     /locus_tag="ACG80L_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5023772.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8624..9253)
                     /locus_tag="ACG80L_001539"
     CDS             complement(8624..9253)
                     /locus_tag="ACG80L_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5023773.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9423..10715
                     /locus_tag="ACG80L_001540"
     CDS             9423..10715
                     /locus_tag="ACG80L_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5023774.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10791..11453)
                     /locus_tag="ACG80L_001541"
     CDS             complement(10791..11453)
                     /locus_tag="ACG80L_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5023775.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11678..11962
                     /gene="groES"
                     /locus_tag="ACG80L_001542"
     CDS             11678..11962
                     /gene="groES"
                     /locus_tag="ACG80L_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS5023776.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12013..13638
                     /gene="groL"
                     /locus_tag="ACG80L_001543"
     CDS             12013..13638
                     /gene="groL"
                     /locus_tag="ACG80L_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS5023777.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13727..14866)
                     /locus_tag="ACG80L_001544"
     CDS             complement(13727..14866)
                     /locus_tag="ACG80L_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5023778.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14977..15561)
                     /locus_tag="ACG80L_001545"
     CDS             complement(14977..15561)
                     /locus_tag="ACG80L_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS5023779.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15688..16119)
                     /locus_tag="ACG80L_001546"
     CDS             complement(15688..16119)
                     /locus_tag="ACG80L_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5023780.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16137..16457)
                     /locus_tag="ACG80L_001547"
     CDS             complement(16137..16457)
                     /locus_tag="ACG80L_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5023781.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT         5405 a         2824 c         3362 g         4928 t
ORIGIN      
        1 attcattatc acgcatttat acgcaaagct cttcaagagg ctgttattac agaattgata
       61 agtacaaatg tagcagataa agtaagaaag ccaaaaaaga aacaatttgt ttctcaagtt
      121 tataatcaac aagagatctt gaaattactt gacattttgt cagaagaaaa actctatttg
      181 gtagttcttt taacagcatt ttatggattg cgaagaagtg aagttttagg attaaaatgg
      241 tctgctattg attttttaaa caaaaaaatc atgataaacc atgtaatcat agaaaatcca
      301 gagaatatgt cacagttaat aaagaaagat caaacaaaga actcttccag ctatcgtaca
      361 ttacctttgg ttgaaacgat tgaacgagca ttagtaaatc aagctaaatg gcaacaagat
      421 aatcgtattc ttttaggaga tgattatatt ttagaagata atgagtacgt atttactatg
      481 gaagatggac ggttgatgaa gcctcaatat gtaacgcatc gtttgagtaa gttgattaag
      541 aaaaatggat tgaaaaaaat tcgctttcat gacttgcgtc attctaatgc ttcaataatg
      601 ttagatagtg gccagaatat gaaatccatt caagaatggc tgggacatgc atcgtattcg
      661 acaactgcta atttatacac gcatttaacc gcaggagtaa aagatcgtgc agcagaagca
      721 ttagaaaacg catttgggtt taaagaataa aaaaacaaaa aacaatatct ttggggtatc
      781 gcttcttgtt tttagaattt ttttgttggc aatttgttgg cagtatgttg gcaatgaaaa
      841 aattcacaaa ctaaaaacct atttataaag ctttataaaa cttttttcac aaatagagca
      901 tttggttaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa aagtacttaa
      961 taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat cagaaaaatc
     1021 tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta ttatataagc
     1081 ataccaacaa acaaagttct tgcaagagaa caaaccctaa cacttttttc attttttact
     1141 cctttcccgc cgtctccccg gcgggtctcc tgttcttaac gataaaagac aagaaggctt
     1201 agccttcttg ttttttgttt tcatggaatc ccgctaatca aatcacctag ttaaagcaac
     1261 tagattgaat gtttatcata agtctgatat catgtttatg aaatgaaaaa aggagcggtt
     1321 atgatggaaa aaatgcgtca gcaattaatg gagtggggga aggaactaga aacattccgt
     1381 ctgcctcact gggaagaact ccctgactta gaactttata tggaccaagt cagaacgtta
     1441 gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc atccattgct tacctcttct
     1501 atggtaaaca attacgttaa actaggtttg ataccagcac cagttaaaaa aagatataac
     1561 aaagaacacg tagcattttt acttgcaatc actacgttga aacaagtatt gactattcct
     1621 gaaatcaaag aagggatatt gttccaagga aaaacagtag gaattcgtga agcctataat
     1681 ctattttgcg atgaacaaga agcagctgtg tggatggtca gccaattagc acaaggtaaa
     1741 tctcatccgc aaaaattcga ccaggcaacc cctgtagaat atattgctgt aagagcggca
     1801 actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga aaccgaatat
     1861 ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca attttagtag attcaggtac
     1921 ggatgttcca gaagaattga taaaaaaata cgagatgtat atgatcccat tgaaaattat
     1981 ttataaagat caggtatata cagataaaat tgacattacc cctgaagaga tctatcaacg
     2041 tttgccagag gaaatccctg gtacatcttt gccggatggt gagtcgatca cagaaatctt
     2101 tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt
     2161 gagcgggaca tataatgtgg ttcgtttaat tgcacaacag caagaagaat tggaagcata
     2221 tgtactagat acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa
     2281 atggctggaa gaaggagtag aatgggatca actgatcagt aatctgaatg agttagtcga
     2341 aagaacaaaa gttttcttta atgttgcgac attggaatat cttcaaaaag gtggacggat
     2401 cggtttagtt gcttctattg taggaaccgc tttaaagctt aacccaatca tctcatgtaa
     2461 tgaagaaggg atttattaca cagtaggaaa agctagggga cgtaaaaaaa gtttggataa
     2521 aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc
     2581 ccatggggat gcgaaagaag aagcaattga aatggaagcg agacttaaaa gagagttccc
     2641 acaagcaaac caaatctact ttggccagat ttctccggca ttagtagtcc acactggtcc
     2701 gggattattg ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa ggaaacactg
     2761 aggtgaaaat cagtgttttc cttttttatt gaagttagaa caaaaaataa taaagtattt
     2821 tgcactttca ttttaatttt ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa
     2881 tacggtataa tgagaaggta tgacaataat atataagagg tggctcttta tgaaaaaagt
     2941 tttagttgtc gatgatgaga agccgatttc ggatatcgtc aaattcaatt tagccaaaga
     3001 aggctatgac gtttatactg catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt
     3061 agaacctgat ttgattttac ttgatttgat gctacctaaa atggacggct tagaagttgc
     3121 tcgtgaggta cgcaaaactt atgatatgcc gatcattatg gtaacagcca aagattctga
     3181 aattgataaa gtcctaggct tagagttagg cgccgatgat tacgtgacca agccattttc
     3241 caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa
     3301 ggaaccagaa gaggcagcac ctgcggaatt gacgattggt gatttgacga tccatccaga
     3361 agcttacatg gtgacgaaac gaggagaaac aatcgagtta acacatcgtg aattcgaact
     3421 actcttttac ttggcgaagc accttggaca agtgatgaca agagaacatt tattacaaac
     3481 ggtttggggt tatgattatt ttggagatgt acgaacagtg gatgtaactg tacgtcgttt
     3541 gcgtgaaaag atcgaagata atccaagcca tccaaattat ttagtgacac gtcgtggtgt
     3601 cggttactat ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa gtccgctttt
     3661 ttcgatcagt caattttaag atcgctatta cctttattct gattttattg atctcgatcg
     3721 aaattattgg tgcctacttt atacgtggtt tagaacgttc cacgatcaat acatttatta
     3781 aagatatgaa tcaaacggtg gaatcattag caacaacaat cagcccggaa ttgaatcgaa
     3841 aagataatgc agatgatgaa gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg
     3901 cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa
     3961 ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat atcaatgatt
     4021 tcacgactaa acgttatgtt gctttagata acgacgataa acgggtgaat atcaatgttc
     4081 aaccgatcct gtccccgacc ggcgatgccg ttatcggggc tctttacgtc aaaagtaata
     4141 tcgaacaaaa atattcggaa atcaataaca cagcagtcat tttctttact gcctcactaa
     4201 ttgcggcatt tatttcaatg atcgtatctg ttttagttgc tcgttcgatt acacagccga
     4261 tcggtgaaat gagagaacaa gccattcgaa tcgctagagg agactacagt cggaaagtca
     4321 aagtgcatgg acaagatgaa cttggacagc tggctgatac cttcaatcag ttagctgaac
     4381 gtattgaaga agctcaagat acaatggaag ctgaacgcaa tcgtttggat agcgtgctat
     4441 cccatatgac agacggcgtc attgcgacag atcgcagggg taaagtcatt acgatcaatg
     4501 atatggctgt ttccttgcta gatatcaaaa acgaggaagc aatcggacaa tcgattttga
     4561 cacttttaga tatcgaagaa gaatatacgt tgcggaaatt attagaagat ccaaacgaaa
     4621 tggtacttga ccgttcaacc agtacattgg aaagtgatca gatgatcttg cgagtagact
     4681 tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc ttgcatgatg
     4741 tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg
     4801 aacttcgtac accgttgaca agtatgcgta gttatatcga agcattgagc gaaggtgcgt
     4861 ggaaagacga ggaagtagca ccaaattttt tgaaggtaac gctagatgaa acagaccgaa
     4921 tgattcgaat gatcaatgac ctcttaaatc tatctcgcat ggatacaggt aatacccagc
     4981 ttcaattaga atatgtcaat ttcaacgagt tagtgaattt tgttcttgac cggtttgata
     5041 tgatggtggg aaatcaggaa aaaaattata agatccgtcg tgaatttacg caaagagatc
     5101 tttgggtcga agtagataca gataaaatca tccaagtagt cgataatatc atgaataacg
     5161 cgatcaagta ttcaccagat gggggaacga ttacttgtcg cttattagaa acgcataata
     5221 acgtgatttt aagtatcacc gatcaaggat tggggattcc taaaaaagat ctgaaccgag
     5281 tgttcgagcg gttctatcga gtagataaag cacgtgcaag agcacaaggt gggacaggac
     5341 ttggattagc tatttctaga gaagtgatta aggcgcatcg aggcgctatt tgggcagaga
     5401 gcaaggaagg aaaagggtcg actttttata tctcgctgcc atatgaacct tatgaggagg
     5461 aatggtggga atgagaatct ctgaaaaagt cgttcgtata ggactcattt tgatggttgc
     5521 gttaagcata tacttctctt atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa
     5581 cgtagatgag acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt
     5641 ctttcttccg cttcatgtta catggcacca ttcgggaatg atccaagaaa ccaacagtga
     5701 aaatctcgtg taccgattgc aattgatcat cgataaagca agatttggaa agctttctga
     5761 agtagtttca ggagacaaat cacagtttga acaggtgaaa aatctgaact ctggttttga
     5821 gttggcctat aatgcgcctt ttttgctaag agaatacaag gaagcatttg atttagattt
     5881 agatgtgtcg aacttggcaa aagaaagcga aaatatgtat tttactagaa tacaattcga
     5941 tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac catctcgtat atgaagcaaa
     6001 tatttcgttg aattggacag atatcaataa agaattaaca gcaaaagaca caaaatggac
     6061 agaaatgaca acagaaccat cgacgatcga cacgcaatat gctacgaaac attcgatcaa
     6121 actgaagaag tatagttata tcctgtcaca gcaaccctat acacttttta gaaatgcttt
     6181 tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc aatgaattga ttttttacgg
     6241 tgggaatgaa acaatgtcta tccattcgga aacgcaagta gttgattttc gtggagagct
     6301 gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt
     6361 aggcagttct ttagggaacc tcagatattt tgaccgcagt gacaatacga tcgattatcg
     6421 gatattcgtg gaaggcttcc cggtttttgg gacagacagc agaggacaga ttggtgtgga
     6481 agttggcggt gaaacgaatg gttctgtgca agtgaatatc caaaccagtt tgaatacgat
     6541 tcaagtgccg ataccttcag acgaagaagt agaacttgca agtacgaatg aaatcatcca
     6601 gcagttaaaa gctaagggag cagatagcaa caagattggt tcagtgatcg ttggctatac
     6661 atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa
     6721 atataaagat aactggtatt cagctaacga ccttctggaa aaattacctg gattggaggc
     6781 gaattaattg gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt tagggttgaa
     6841 tgtgttttta ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg tcattcgctc
     6901 taatcagact gaatcaatcc aacaacgtct ggctagcgac gatatcactt acacggggaa
     6961 gatttcgtct gagaacaagc aaggctatta tcttagcgcg gagcagacca atcttagcac
     7021 ttcattagcg aattatcgca aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt
     7081 agacggtgat gttttgacga aatctttatc cagttcagat gctgaaaaga acgtgatcga
     7141 tccagataaa attagtagtt ccttggaaga ttttttatct aacaaagaca atattttatt
     7201 cggtaatgat tatgtgtaca cgaaaaactt ttctcattta gaagaagaac cactagaaat
     7261 ccaagcaagc caagagtatg aaggcatccc attcaatgat gatacagcaa aaatcgttct
     7321 ttctttagaa aaaaacgata atgattatag catcacgaaa tacatgcaga cgcatttatc
     7381 tgatatcgag cagctgcgcg aaaagacgga actgtatacg gaagaggaag caatcaatac
     7441 attgtacatc aataacaaaa taccaagagg atcaaaaatt ttatggcgtc agctagctta
     7501 ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat
     7561 cgaaacaagc gataaagtgg tccaaattga gagcgtcaac gctttcagca atacgatcgt
     7621 gacaaacaat actgtaccaa aagtggaaga tcaataaaaa agcgagtata atgaaaaaag
     7681 tttacaaagt aagaagggaa gtaggagtca aatggctgaa gaaaaaccag cttttaaaat
     7741 cagtatccta gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg
     7801 gcttttggtc gatgcaggac ttagcggtaa aaaaatcact tccttgatgg ctcaaattga
     7861 tcggacaccg gaccaattag atggaatact tgtgacgcat gaacatcgag atcatataca
     7921 tggcgtgggt gtcttggctc gtaagtatca tttggatgtt tttgccaatc agcagacttg
     7981 ggaagcgatg gaaccactga ttggaaatgt accagtggaa caaaaacatc tatttgagat
     8041 ggggaaagtc agaacattcg gcgatatcga tattgagagt ttcggcgtct cccatgacgc
     8101 agcggctccg caattttatc gtttccataa agaaaatcgg tcattcgtca tgttgacgga
     8161 caccggatat tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg gttatttgat
     8221 ggaaagtaat catgatcttg agatgttacg catgggacct tatccgtgga atttaaaaca
     8281 gcgtatttta ggagatagag gacatctttc caacgaagac ggtgcacttg tcatgactga
     8341 tgtgatcggt aatcggacca aacgaatcta tcttggccat cttagcaagg aaaacaatat
     8401 gaaagaactt gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg
     8461 acatgatttt gaaatctatg atacagatcc agatgaagca actgagctat tttcgattta
     8521 aacagttcaa tcaataaaaa tataaaaagc agaagataaa atcaatcagg gttggtttta
     8581 tcttctattt ttattttttt agcatattaa aaaaataacc gtattatcga ctgactacac
     8641 tgatgtgctg cttttcatgt ttctcatcca gtatctcatc tactaatgca atcgcgtaat
     8701 ctgccatgct gatcgtactg tcgctagcag cgtttctttg taaataatca tcacttaaca
     8761 aataagtacc tgtacgtttg ccatgtggat caaataaaga agctggactg atataagtcc
     8821 aatgtagatt tttagattgt ttcaatttta acaatccttc gtacatattg aaagctgttg
     8881 gatagaaggg tgcattttta ggtacttggt caatcatacg agtcgtcttt tctgtatcta
     8941 agaataacga agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct
     9001 caatcaaatg ggacaaagaa gtttgatgca tttcttcttt tcctttggga gcgttgaaag
     9061 catcaacgac aacatcaaaa gatgcaagat cagtagttgt caactcaaat aaatcctttt
     9121 gtataaacgg aacatctgat ggcaattttt ctggatgacg cacgattgcc gtcacgtcta
     9181 aatctctgga caatgcttcg tctaaaataa atgaacctgc atggccagta gctccaatga
     9241 gtgcaatttt cataaaaaac ctcctttttt attcctgtcc attgtaagca gttgctgaat
     9301 attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat
     9361 cattgggtat agttaaatta agaaaactga ttgataaaaa tagaagttga aggagataag
     9421 aaatgaaaga gtggttagat cgtgcctttt taccaatgat aacatctgtt gtgaatctta
     9481 gacccttaac tgctttgcgt aatggaatga cttacacgat cccaataatt attgttggct
     9541 cgctgttttt cttatttgct cattttcctg tggattacat cgctcagtgg atagacaaaa
     9601 caggattttc tatgtatttt acacaagtat accaggctac atttgatgtg atagcctttt
     9661 ggatcgtttt tgccattgca tacgcttatg caaaagaaga agacatcgat cgcttaccag
     9721 tcggcataac agccttgtct gcttatctac tgcttattca gccactaaat actcagatag
     9781 atactgtctg gacaggtgct gaagggatac taggagcagt tacaactggg ctcatcatcg
     9841 gatggggcta tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga gagaagatac
     9901 ctgaaaatgt ttggcagtct tttacttcat tgattccaat cattgttata gtcactttta
     9961 cgcttatcgg ttcagcggtt tttcaacatt tgttcagaat gactgttgtt caaggagtat
    10021 ggacatatgt ccagcttccc attcaatcgt tcatagacac gttgtttggt gtgcttttat
    10081 taggtttttt tgttccgttt ttatggttat ttggtcttca tggatctgcg atggtcaatg
    10141 ggttggttag tcccgtcttg caagcgaatt cattagcaaa tgcagaaatc ttagcctcag
    10201 gacaagagtt gacggtggca aatggcggac atatcgttac tcagcaattt ctcgatcagt
    10261 tcatgacggt tacgggtgct ggattgacgt taggggctgt attttttatg acggtttttg
    10321 cgaaatcgaa gaaatacaga gaattaggaa atcttgctct tttaccagcc tttttcaaca
    10381 tcaatgaatc aattattttt tccacaccga tcgtgatgaa cccgatgatg gctgttcctt
    10441 ttattttcgc accggtcgtt tcaggattga ttacctattc cgctttatat tttggattcg
    10501 ttcctttatt tacagcagtc caagtccctt ggacaacacc gccgatcctc tcgggtttct
    10561 tagtaggcgg agtgcctgca gcgattttac aaggtatcgt gttgtcaatt agttttttca
    10621 tttatttccc atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa agacagacaa
    10681 aaaatgatgg agcagcagaa aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt
    10741 agattgggaa aatcaccaat ctaggatgac aggagtattt atagtcatgt ttatactagc
    10801 cctttttgga cagctaattg aacaagaaca actaatgtgt tcattcctac gtgtgtgatc
    10861 atcgatgtcc agatccgacc agttgctttg tactctaggc tgaagaagaa acctaggaaa
    10921 aagtagatta gcagatgacc atcattatga gcaaacgcaa acagcaaaga gctgatcact
    10981 gctccgaccc aaaaatttga atatcgactg atgatcccca gtatactgcg gcggaatacg
    11041 aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg
    11101 atcatctgga tgatattttg tgtattctcg gaaggggttg ctttgccaaa caatacagat
    11161 tctaacatga cagcgatgct ttgcagaatc aatgcgacaa agattccaac caatccataa
    11221 ataacaatct ttggagcaga agcttttttt accgctgatt cgatcgctaa gggttcacgt
    11281 tgcttaaaat aaaaaatagc taggacaacg gcgcctagta tatacatgac tgcagttgct
    11341 gtgatcaatt cagacgtaga ttttacaagt cctatcgaag ctaggatcaa gggtgagata
    11401 aaaattagac catagcaaaa aatgctgagg aagctatatt tttttaaaga catgtaattc
    11461 ctccttttaa gggataacaa gcatgcaagg caagcttata tcgattgatt tcaattatat
    11521 cataatttca ataagcagct gtcagaataa aatttctttt ctttgattta aaagcttgca
    11581 aaaaagaggg agaatgagta ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc
    11641 taacagatta aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat taggtgatcg
    11701 cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc
    11761 tgctgctaaa gagaaacccc aaactgggac agttgttgca gtcggtgaag gccgtttgtt
    11821 agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa
    11881 atacgccggt acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga
    11941 tatcatggcg attgttgagt aaatttggaa gataaaaaaa taatcaaaaa taatcaatga
    12001 ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc agctatgtta
    12061 cgcggggtag ataaattagc agatacagtg aaagtaacat taggacctaa aggccgaaat
    12121 gtcgtactag aaaaatctta cggctctcca ttgatcacaa atgatggtgt gacgatcgca
    12181 aaagaaatcg aattagaaga tcattttgaa aacatgggtg caaaacttgt atcagaagta
    12241 gcatcaaaaa caaatgatat tgcaggagac ggtacaacga cagcaactgt tctgacacaa
    12301 gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct aggcattcgt
    12361 cgcgggatcg aattggcaac aaaagcagca gtcgaagaat tgcataatat ctcaactgtt
    12421 gttgattcaa aagaagccat tgcacaagta gcagctgttt cttcgggttc tgataaagtt
    12481 ggacatttga ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa
    12541 gaatcaaaag ggatcgaaac agaactagat gttgtagaag gtatgcagtt tgatcgtggt
    12601 tacttatcac aatatatggt aacagataac gacaaaatgg aagctgttct agaaaaccca
    12661 tatatcttga tcacagacaa aaaaatctct aacatccaag atattctgcc attgttagaa
    12721 caaatcttgc aacaatcacg tccattgttg attattgctg acgatgtaga tggtgaagca
    12781 ttgccaactc ttgtattgaa caaaatccgt ggaacattca acgtagtagc tgtaaaagct
    12841 cctggtttcg gcgatcgtcg taaagcaatg cttgaagata tcgctatttt gacaggtggt
    12901 acagtaatta ctgacgatct aggtttagaa ttgaaagatg caacaatcga aaacttagga
    12961 aatgcttcta aagttgttgt agataaagac aatacaacga tcgtagaagg ttctggagaa
    13021 aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga aacaacttct
    13081 gattttgacc gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt
    13141 gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat taaaattacg cattgaagat
    13201 gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct
    13261 ctagtgaacg ttatcagcaa agtttctgct gtagaagcag aaggagacgt ggcaacaggt
    13321 atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga aaacgctggt
    13381 tatgaaggat cagtgattgt tgataaattg aagaacgtgg aactaggcac tggtttcaat
    13441 gctgctactg gagaatgggt aaacatggtt gaagcaggta tcgtagaccc aactaaagta
    13501 acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac gacagaagca
    13561 gtcgttgcag ataaaccaga accagcagcg ccagcagctc ctgcaatgga tccatcaatg
    13621 atgggcggta tgatgtagta attaagaaag aaccctattt taacagggtt ctttcttctt
    13681 tttttgtttt attttaagca aaaggggcaa agaaggggca aaaatatcaa aggctattta
    13741 gtttatcaat tacttgttgt ttggctcttt ttgttacatg gttataaata gacagagttg
    13801 tgtttgcatc agaatgccct acacgctcca taatggcttt aagaggtacg cctaattcag
    13861 ataatagtga aacgtggcta tgtctgaata tatgtgagga tagacttttt tctaattcca
    13921 actcttcctc tactttatgg agtatagcgt taaatgagtg cagcgcaagc ggagtgccac
    13981 ttgtagatat aaatatatat tgatcgggat ctgtgggtct acctgcaaga atattgtcag
    14041 ctatcacact ttcaatcaat tcttttgcac gattgggtaa ttgcacttca cgttgcgaat
    14101 aagtattttt tggagttgtt tttatagcat tatccatttt cacagatgtg taatctaagg
    14161 tcccattaat ggaaattttc ccatcctcat agtccttcat ttgcaaagct agcaattccc
    14221 catatctcaa accagttaaa tatagaaact cagctattat gccgtgtagt tttctgcgag
    14281 gattggagta tagctgtttc agtatttgat caatttcttc tttctccaga tattttttat
    14341 tcatagaaag ccttcttttt tcttcttcca cttttttagg atggatttta actgctagcg
    14401 cagggtttct ttgaatgtat tttctatcga ttgcatagtt tagcataaca gatagagttg
    14461 tttttgtttg ttttgtgtag ttcagtgaga ggtcaccaaa cgtatacata tcttcaatta
    14521 tcttattaat gagtgtctca tcaatgtttc taacgatcgt atcatcgctt atgtgcttag
    14581 aaacatgttt catcatcatt ggaaccttca aatagctagt acgtttaaca tgctgcttat
    14641 aatattcata ccattcttta tacagctcac caaaagtgat atctgattta ttgtaatctt
    14701 cgagtgcttc tttaattttt ttatctagaa ttttctgagc ttttttccac gcttgtggtg
    14761 aattacttgt aagtgttgta gatttttttc gtgttttttc tgtataagga tctacatatc
    14821 tttcaataaa cttgaattgg ccatctttgg tctgttcaac ccacactttt aacatctcct
    14881 atcatttgct ataataggca taacaaatag acctatatag gtttgttttc taaaagcacg
    14941 ctcttactct ggacggtggg gcgtgttttt tatttattat acagttgctt taaaaatagc
    15001 tgctttttca aatgatacta aaggagagaa gtggatttct atttctccct tttggtttaa
    15061 cgtgaaatga gcagttacat ccatttgttt tcctggagca acagatccca ttgtattttc
    15121 gttggcatat gtctctgatt ttttgtcatc tggtccatat acttccacat ctgtacctac
    15181 agggatatct gaatcgccat cattttttac ggtataagta atttttacta cttgtgcagg
    15241 ttggttttct tcaaattgat ttctttcatc agttagttct acactattta gtgtatattc
    15301 agcatcacca acttttacag tgtcaccaat cttatagaag gtatcgcttt tttcttcttt
    15361 agaagatgaa gcagaggttg aagatttagt tactttttcg ccaccattat cacttgcttt
    15421 attactatct gatccaccat ttaaagcaga accaataatt ataattaaaa ttactgctaa
    15481 tacccaaaac catacttttt tgtaaaaagg tttactaact ttatacattt ttccgtcttg
    15541 acccataact ttttttgcca tttaaatatt cctcacttct tgttataata tatttgcgat
    15601 ctcagaaatg aggtatgagt ccgtgtgcca gcacgggctt ttttaatgtt ttgctgaaat
    15661 cggttttttt aataattctt gatgtattca tacatattgc cttgcgtaag aatatttttt
    15721 cttaaaatag cattggcaga cagcataaca agtgtatctg agcttattct gaatagaata
    15781 atactccata aatttttcga gattaaactg agattcatca gtcagttcgt tctcaatata
    15841 gatatttaat aaaattagaa tagctatttt atctgcttct gtttcaaatt ttgagtgaaa
    15901 agttgtagaa gtatcgtaca aaactgaata ttcaaaatga gaagcggtga aatgcgcaag
    15961 ttcatgagat aaatgaaagg cttctgcagt ttcgccatat agattttcat tcaaaaaaat
    16021 gattctaggc ttcggataat agaagccagg ctctttcatt tccatgtaaa ctactttcaa
    16081 attgtattcg ctcaacattt ctttcaattt caaatacata caacccatca ctccaactat
    16141 tcattttcct ctaaagcttt agcaattgca atcgctttac gcattgtctc cttagatatt
    16201 tcttttccat caaaagaaaa aacagtatcg ttttctgata aatccacatg tttaggggtt
    16261 tctctttctt ctctacctag aaggtagtct acagagactc caaagtaatc agctaaccga
    16321 gctaactcaa tagaattagg agtttgtctt ttccaacttc ctaaatatcc attcgaatac
    16381 ccgaaattca tttcgagctg tctaatggat aatccttttt cttttgctaa ctcctttatt
    16441 atttcgtaag tattcattga taaatcaacc tttctgaatg cttacaaaaa aagtttagat
    16501 aaataatgta aaaatagtt
//
LOCUS       DAZUMB010000054        16492 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000054 DAZUMB010000000
VERSION     DAZUMB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.054"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1452
                     /locus_tag="ACG80L_001548"
     CDS             <1..1452
                     /locus_tag="ACG80L_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5023782.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="ACG80L_001549"
     CDS             1801..2433
                     /locus_tag="ACG80L_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS5023783.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="ACG80L_001550"
     CDS             3021..4460
                     /locus_tag="ACG80L_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5023784.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="ACG80L_001551"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="ACG80L_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5222..5437
                     /locus_tag="ACG80L_001552"
     CDS             5222..5437
                     /locus_tag="ACG80L_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023785.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="ACG80L_001553"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="ACG80L_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="ACG80L_001554"
     CDS             complement(6097..7008)
                     /locus_tag="ACG80L_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5023786.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="ACG80L_001555"
     CDS             complement(7027..8031)
                     /locus_tag="ACG80L_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5023787.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="ACG80L_001556"
     CDS             complement(8028..10151)
                     /locus_tag="ACG80L_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5023788.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="ACG80L_001557"
     CDS             complement(10156..12603)
                     /locus_tag="ACG80L_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5023789.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="ACG80L_001558"
     CDS             complement(12590..12979)
                     /locus_tag="ACG80L_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5023790.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="ACG80L_001559"
     CDS             complement(13039..13542)
                     /locus_tag="ACG80L_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5023791.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13779)
                     /locus_tag="ACG80L_001560"
     CDS             complement(13555..13779)
                     /locus_tag="ACG80L_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023792.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="ACG80L_001561"
     CDS             complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="ACG80L_001561"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5023793.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4913 a         3271 c         2939 g         5369 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    13741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    13801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    13861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    13921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    13981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    14041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    14101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    14161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    14221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    14281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    14341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    14401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    14461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    14521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    14581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    14641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    14701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    14761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    14821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    14881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    14941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    15001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    15061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    15121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    15181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    15241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    15301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    15361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    15421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    15481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    15541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    15601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    15661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    15721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    15781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    15841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    15901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    15961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    16021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    16081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    16141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    16201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    16261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    16321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    16381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    16441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt tt
//
LOCUS       DAZUMB010000055        16363 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000055 DAZUMB010000000
VERSION     DAZUMB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.056"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ACG80L_001562"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ACG80L_001562"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS5023794.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ACG80L_001563"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ACG80L_001563"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS5023795.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ACG80L_001564"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ACG80L_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS5023796.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ACG80L_001565"
     CDS             2857..3732
                     /locus_tag="ACG80L_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS5023797.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG80L_001566"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG80L_001566"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS5023798.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ACG80L_001567"
     CDS             4629..5063
                     /locus_tag="ACG80L_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS5023799.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ACG80L_001568"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ACG80L_001568"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS5023800.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ACG80L_001569"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ACG80L_001569"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS5023801.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ACG80L_001570"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ACG80L_001570"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS5023802.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ACG80L_001571"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ACG80L_001571"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS5023803.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ACG80L_001572"
     CDS             13334..14260
                     /locus_tag="ACG80L_001572"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS5023804.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ACG80L_001573"
     CDS             14442..15335
                     /locus_tag="ACG80L_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS5023805.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ACG80L_001574"
     CDS             15469..15594
                     /locus_tag="ACG80L_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS5023806.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ACG80L_001575"
     CDS             complement(15728..16360)
                     /locus_tag="ACG80L_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023807.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5811 a         2788 c         3492 g         4272 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aat
//
LOCUS       DAZUMB010000056        16218 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000056 DAZUMB010000000
VERSION     DAZUMB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.057"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACG80L_001576"
     CDS             complement(29..727)
                     /locus_tag="ACG80L_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS5023808.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACG80L_001577"
     CDS             complement(720..2072)
                     /locus_tag="ACG80L_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS5023809.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACG80L_001578"
     CDS             complement(2295..3695)
                     /locus_tag="ACG80L_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5023810.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACG80L_001579"
     CDS             3856..4593
                     /locus_tag="ACG80L_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS5023811.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACG80L_001580"
     CDS             4718..5197
                     /locus_tag="ACG80L_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS5023812.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACG80L_001581"
     CDS             5194..5961
                     /locus_tag="ACG80L_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS5023813.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACG80L_001582"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACG80L_001582"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5023814.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACG80L_001583"
     CDS             complement(6864..7466)
                     /locus_tag="ACG80L_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5023815.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACG80L_001584"
     CDS             7572..7802
                     /locus_tag="ACG80L_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023816.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACG80L_001585"
     CDS             7793..8041
                     /locus_tag="ACG80L_001585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5023817.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACG80L_001586"
     CDS             8147..8731
                     /locus_tag="ACG80L_001586"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS5023818.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACG80L_001587"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACG80L_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS5023819.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACG80L_001588"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACG80L_001588"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS5023820.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACG80L_001589"
     CDS             10632..10811
                     /locus_tag="ACG80L_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023821.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACG80L_001590"
     CDS             10833..11858
                     /locus_tag="ACG80L_001590"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS5023822.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACG80L_001591"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACG80L_001591"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS5023823.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACG80L_001592"
     CDS             13430..14377
                     /locus_tag="ACG80L_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS5023824.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACG80L_001593"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACG80L_001593"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS5023825.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACG80L_001594"
     CDS             complement(15279..15521)
                     /locus_tag="ACG80L_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023826.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACG80L_001595"
     tRNA            15642..15714
                     /locus_tag="ACG80L_001595"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..>16218)
                     /locus_tag="ACG80L_001596"
     CDS             complement(15833..>16218)
                     /locus_tag="ACG80L_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS5023827.1"
                     /translation="SVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEELFSVYFL
                     AIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFNQAAIKS
                     NSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5525 a         2899 c         3276 g         4518 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgaga
//
LOCUS       DAZUMB010000057        16068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000057 DAZUMB010000000
VERSION     DAZUMB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.058"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1400
                     /locus_tag="ACG80L_001597"
     CDS             <1..1400
                     /locus_tag="ACG80L_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5023828.1"
                     /translation="LRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLNDINNIKKEFE
                     EQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTECE
                     FISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPE
                     SFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKR
                     PIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDN
                     TPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNL
                     YKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLI
                     YREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPIS
                     INTIDRAIISAVQNK"
     gene            complement(1492..1857)
                     /locus_tag="ACG80L_001598"
     CDS             complement(1492..1857)
                     /locus_tag="ACG80L_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5023829.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1854..2219)
                     /locus_tag="ACG80L_001599"
     CDS             complement(1854..2219)
                     /locus_tag="ACG80L_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5023830.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2411..2659)
                     /locus_tag="ACG80L_001600"
     CDS             complement(2411..2659)
                     /locus_tag="ACG80L_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023831.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2658..2786
                     /locus_tag="ACG80L_001601"
     CDS             2658..2786
                     /locus_tag="ACG80L_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS5023832.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2843..4537
                     /locus_tag="ACG80L_001602"
     CDS             2843..4537
                     /locus_tag="ACG80L_001602"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS5023833.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4604..4810
                     /locus_tag="ACG80L_001603"
     CDS             4604..4810
                     /locus_tag="ACG80L_001603"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023834.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5188..6321
                     /locus_tag="ACG80L_001604"
     CDS             5188..6321
                     /locus_tag="ACG80L_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS5023835.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6352..6672
                     /locus_tag="ACG80L_001605"
     CDS             6352..6672
                     /locus_tag="ACG80L_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS5023836.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6701..7165)
                     /locus_tag="ACG80L_001606"
     CDS             complement(6701..7165)
                     /locus_tag="ACG80L_001606"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5023837.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7297..8550)
                     /locus_tag="ACG80L_001607"
     CDS             complement(7297..8550)
                     /locus_tag="ACG80L_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5023838.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8579..8896)
                     /locus_tag="ACG80L_001608"
     CDS             complement(8579..8896)
                     /locus_tag="ACG80L_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5023839.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8922..9242)
                     /locus_tag="ACG80L_001609"
     CDS             complement(8922..9242)
                     /locus_tag="ACG80L_001609"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5023840.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9706..11121)
                     /locus_tag="ACG80L_001610"
     CDS             complement(9706..11121)
                     /locus_tag="ACG80L_001610"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5023841.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11138..12454)
                     /locus_tag="ACG80L_001611"
     CDS             complement(11138..12454)
                     /locus_tag="ACG80L_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5023842.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12604..13302)
                     /locus_tag="ACG80L_001612"
     CDS             complement(12604..13302)
                     /locus_tag="ACG80L_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS5023843.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13591..14580)
                     /locus_tag="ACG80L_001613"
     CDS             complement(13591..14580)
                     /locus_tag="ACG80L_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5023844.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14660..15493)
                     /gene="msrA"
                     /locus_tag="ACG80L_001614"
     CDS             complement(14660..15493)
                     /gene="msrA"
                     /locus_tag="ACG80L_001614"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5023845.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15668..>16068)
                     /locus_tag="ACG80L_001615"
     CDS             complement(15668..>16068)
                     /locus_tag="ACG80L_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023846.1"
                     /translation="TTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTG
                     SSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVS
                     TSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         4991 a         3249 c         2734 g         5094 t
ORIGIN      
        1 ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa
       61 agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa
      121 aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat
      181 atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt
      241 ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg
      301 aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttact
      361 ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac
      421 gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg
      481 aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat
      541 tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc
      601 agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta
      661 tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag
      721 aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc
      781 agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa
      841 taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac
      901 cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca
      961 tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga
     1021 caattttttc cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca
     1081 tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta
     1141 tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg
     1201 taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga
     1261 agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg
     1321 cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt
     1381 cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt
     1441 gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt
     1501 ttggacatct aggggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac
     1561 taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat
     1621 cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat
     1681 agcattgttc tcgcttactc ggtcgctttg cacttttgta tggacaaata aatatttcaa
     1741 aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag
     1801 tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct
     1861 acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg
     1921 actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata
     1981 actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct
     2041 agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga
     2101 gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa
     2161 caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata
     2221 tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt
     2281 gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact
     2341 ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt
     2401 aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg
     2461 tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc
     2521 tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt
     2581 ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa
     2641 acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc
     2701 ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt
     2761 tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt
     2821 ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat
     2881 tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat
     2941 caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt
     3001 ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg
     3061 cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc
     3121 ttctgttact gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca
     3181 ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa
     3241 actgggattc aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc
     3301 agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt
     3361 ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc
     3421 tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca
     3481 tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat
     3541 cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga
     3601 attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac
     3661 actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa
     3721 tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat
     3781 ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg
     3841 aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac
     3901 gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa
     3961 tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa
     4021 tatgtctatt gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt
     4081 tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat
     4141 taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc
     4201 tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat
     4261 ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc
     4321 aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc
     4381 ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca
     4441 ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc
     4501 tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc
     4561 cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt
     4621 ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagc
     4681 tgccctgcat caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg
     4741 gttagactta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa
     4801 aggcgtttag tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca
     4861 taaatttgac ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt
     4921 ctgtttcttt ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca
     4981 tctaacggat acaactattt caatgattac cattacacta aaaaatcgga aacacacctc
     5041 tccgaacaca aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta
     5101 tcaaaaaggt aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat
     5161 tttatgaatc tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa
     5221 atggaaatca gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt
     5281 atcacagcaa cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt
     5341 ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct
     5401 caagttcaaa cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt
     5461 cattgtcatt tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag
     5521 ttatatggcg ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg
     5581 gaatatctaa gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt
     5641 cctgatgaaa aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa
     5701 aacgcaggag taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta
     5761 ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta
     5821 cagcaccgtc tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac
     5881 acggcacaag ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc
     5941 gattgtgtag gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc
     6001 ttacaaagcg gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga
     6061 acagtccaag taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt
     6121 tttatgcggg atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa
     6181 atttacgcaa tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg
     6241 ccactatcag aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata
     6301 aaagttgttt tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa
     6361 atcaaacgaa atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga
     6421 cgtcgcttcc gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta
     6481 ttaaccggga ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga
     6541 tatggcacct atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga
     6601 atcgtaagga acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa
     6661 aaagaggcat gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt
     6721 gatacagacg agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt
     6781 atcgttcata aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat
     6841 gcatttcttt tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg
     6901 gttcaacaaa cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt
     6961 caatgacgcc cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag
     7021 gaatactttc cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa
     7081 ctccccattt tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta
     7141 atttgatcca gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa
     7201 aaattataca agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa
     7261 cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct
     7321 tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa
     7381 attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat
     7441 tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc
     7501 aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg
     7561 gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc
     7621 aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc
     7681 taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg
     7741 tttgttcacg ataaacggtg cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat
     7801 ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa
     7861 gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag
     7921 tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa
     7981 tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa
     8041 tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt
     8101 ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat
     8161 aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga
     8221 aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa
     8281 gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc
     8341 tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa
     8401 gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga
     8461 gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg
     8521 tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt
     8581 acgcgttcaa tcgacgatac agattaatga tctcttttgc taaatctgta aaagctatag
     8641 atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt
     8701 gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg
     8761 catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt
     8821 ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat
     8881 ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt
     8941 tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca
     9001 tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact
     9061 tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa
     9121 gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt
     9181 gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc
     9241 attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt
     9301 taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac
     9361 actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta
     9421 acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat
     9481 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt
     9541 tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg
     9601 tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt
     9661 tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc
     9721 gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt
     9781 caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa
     9841 tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc
     9901 gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta
     9961 atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc
    10021 gttttctgtt atcagccaag ggatattatg ataattttct tttagataca tggctatatc
    10081 atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa
    10141 atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg
    10201 aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg
    10261 ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa
    10321 ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata
    10381 aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac
    10441 catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc
    10501 tactgcttta gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt
    10561 ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc
    10621 gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata
    10681 cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa
    10741 agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga
    10801 gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc
    10861 agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat
    10921 cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc
    10981 attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc
    11041 tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt
    11101 ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac
    11161 gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt
    11221 gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca
    11281 ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac
    11341 caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca
    11401 tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata
    11461 acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac
    11521 cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa
    11581 tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa
    11641 gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga
    11701 cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg
    11761 ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga
    11821 taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac
    11881 tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc
    11941 tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt
    12001 tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga ttttcattca
    12061 ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga
    12121 atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga
    12181 tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta
    12241 aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa
    12301 acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta
    12361 tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta
    12421 atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc
    12481 tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt
    12541 tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa
    12601 ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat
    12661 atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat
    12721 ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa
    12781 caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc
    12841 ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg
    12901 aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc
    12961 atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc
    13021 tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc
    13081 tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc
    13141 tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc
    13201 atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat
    13261 cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc
    13321 atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta
    13381 tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa
    13441 gactaggata aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat
    13501 gatgttcaat aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat
    13561 atatggataa aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc
    13621 ttcttctggt gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa
    13681 ccgacttgct ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc
    13741 catttgcgct aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc
    13801 cagagttgat ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga
    13861 atcttcagga ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa
    13921 cgtgattgcc cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc
    13981 acgagaaaac atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa
    14041 aatttcagtc attttttttg tatcttctcc attatagttg gatataccaa tatataatgc
    14101 ttttccttgt cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg
    14161 gtctgggcga tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg
    14221 gtcacaactt gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat
    14281 atgataacct gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct
    14341 aaaaatacgc ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag
    14401 gtcgaaatgg gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat
    14461 aggatcataa tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc
    14521 tgaatgaccg gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa
    14581 tttttctcct cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact
    14641 acattttaaa gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt
    14701 tttttactcg ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt
    14761 tcggttgttt tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg
    14821 gttcgatttt tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg
    14881 attgttccgc taattctttt tgtcgttcga cttgataaaa aatcactgga cgatattgct
    14941 tccctctatc ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg
    15001 tcagcaactc gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat
    15061 gacctgtgta accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt
    15121 atcctgacaa aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga
    15181 aacatccccc tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg
    15241 aaaagtcata tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta
    15301 catcaggcgt cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata
    15361 actgttcttt aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc
    15421 gttcccaatc tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt
    15481 gttcctcatc catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta
    15541 atttatcata aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa
    15601 aaaagagaga atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa
    15661 agttaattta ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg
    15721 taacaagata agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa
    15781 ctcctttgac tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt
    15841 gatgatttat ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc
    15901 tcttctcatt agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta
    15961 cccattgact gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag
    16021 cgataggaga ataatcatat aaaaaataat ccattccttt agtggtat
//
LOCUS       DAZUMB010000058        15945 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000058 DAZUMB010000000
VERSION     DAZUMB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.059"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(380..802)
                     /locus_tag="ACG80L_001616"
     CDS             complement(380..802)
                     /locus_tag="ACG80L_001616"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS5023847.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(816..2222)
                     /gene="atpD"
                     /locus_tag="ACG80L_001617"
     CDS             complement(816..2222)
                     /gene="atpD"
                     /locus_tag="ACG80L_001617"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS5023848.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2245..3147)
                     /locus_tag="ACG80L_001618"
     CDS             complement(2245..3147)
                     /locus_tag="ACG80L_001618"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS5023849.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3161..4717)
                     /gene="atpA"
                     /locus_tag="ACG80L_001619"
     CDS             complement(3161..4717)
                     /gene="atpA"
                     /locus_tag="ACG80L_001619"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS5023850.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4740..5282)
                     /locus_tag="ACG80L_001620"
     CDS             complement(4740..5282)
                     /locus_tag="ACG80L_001620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS5023851.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5269..5793)
                     /gene="atpF"
                     /locus_tag="ACG80L_001621"
     CDS             complement(5269..5793)
                     /gene="atpF"
                     /locus_tag="ACG80L_001621"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS5023852.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5884..6099)
                     /gene="atpE"
                     /locus_tag="ACG80L_001622"
     CDS             complement(5884..6099)
                     /gene="atpE"
                     /locus_tag="ACG80L_001622"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS5023853.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6152..6871)
                     /gene="atpB"
                     /locus_tag="ACG80L_001623"
     CDS             complement(6152..6871)
                     /gene="atpB"
                     /locus_tag="ACG80L_001623"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS5023854.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7173..7637)
                     /gene="smpB"
                     /locus_tag="ACG80L_001624"
     CDS             complement(7173..7637)
                     /gene="smpB"
                     /locus_tag="ACG80L_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS5023855.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7659..10016)
                     /gene="rnr"
                     /locus_tag="ACG80L_001625"
     CDS             complement(7659..10016)
                     /gene="rnr"
                     /locus_tag="ACG80L_001625"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS5023856.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10019..10774)
                     /locus_tag="ACG80L_001626"
     CDS             complement(10019..10774)
                     /locus_tag="ACG80L_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5023857.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10877..11113)
                     /gene="secG"
                     /locus_tag="ACG80L_001627"
     CDS             complement(10877..11113)
                     /gene="secG"
                     /locus_tag="ACG80L_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS5023858.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11231..11509)
                     /locus_tag="ACG80L_001628"
     CDS             complement(11231..11509)
                     /locus_tag="ACG80L_001628"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5023859.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11622..12590)
                     /locus_tag="ACG80L_001629"
     CDS             complement(11622..12590)
                     /locus_tag="ACG80L_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5023860.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12805..12849
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12873..13847
                     /locus_tag="ACG80L_001630"
     CDS             12873..13847
                     /locus_tag="ACG80L_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS5023861.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13852..14427
                     /locus_tag="ACG80L_001631"
     CDS             13852..14427
                     /locus_tag="ACG80L_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS5023862.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14494..15633)
                     /locus_tag="ACG80L_001632"
     CDS             complement(14494..15633)
                     /locus_tag="ACG80L_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS5023863.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4356 a         3440 c         2854 g         5295 t
ORIGIN      
        1 agaatgtggt tcattctgac tcagatttga ttttgcacaa ctcatcagtc cttattgaca
       61 aagagcccaa aagtcttgtc gcagtccctt attccaaata aacggtgttc aatcatctga
      121 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttccat ttgtttctct
      181 aatactcagg acaaaacgct gatttcacaa cctccgatat acggtgttcc agaagtagct
      241 tcttcgaaaa ataagctgag atttcacaaa aatttgagaa gcaattttcg taaattcctt
      301 cttatgtctt gaagctgacc acttctgtca taacctcttt ttactgttct taaacacgga
      361 taaacatccg catctcattt tatgagtgct tagatacttt gatacgattg attgctcggt
      421 gcaacgcaac agttgcccgt ttcaattcgt tcgtatcttg ttttgctttt gcttcttcga
      481 tttggcgttc tgcgcgttgt tttgcacgtt ccgcacgaga tacatcgata tcacgttcac
      541 gttccgcact atcagcaacg attgaaacaa cattatcacg aacttccatg attccgccat
      601 taacagcgat ccaatcgacg tgggtatctg aatctgttcg ttttacacga acttcatcaa
      661 tcgtcaatgg aacgatgatt ggtgcatgtt taggcaaaat cccaagctca ccagctgtcg
      721 tacgtgcaac gacgatagct gcatggtgat catagaccaa accggcagga gttaccacat
      781 ttactgttaa atattgatcc atggggcacc cctttctagt agcctagtgt tttcgctttt
      841 tctacgacgt cttcgattcg gccaacactt cggaaagctt cttcaggaag atcatcgtat
      901 ttgccttcca agatttcgcg gaatcctttc actgtttctt ctacagggac atatgaacct
      961 ggaagaccag taaattgttc agccacgtta aagttttgtg acaagaagaa ttggactctg
     1021 cgggcacgag aaactaacac tttttcttga tcggataatt catccatacc taagatagca
     1081 atgatatctt gtaattcacg atatcgttgc aatacgtgtt ggacttctgt tgctacttta
     1141 taatgttctt ctcccacgat ttcaggtgct aaagcacttg aagaagaagc caatggatct
     1201 accgctggat aaatcccttg ttctgttaat ttacgttcca agttggttgt cgcatccaaa
     1261 tgggcgaatg ctgttgccgg cgccgggtca gtgtagtcat cggctggcac atagattgct
     1321 tggatcgatg tgatcgaacc ttttttcgta gatgtgatac gttcttgcaa ttgacccatt
     1381 tctgttgcca atgttggttg ataaccaacc gcagaaggca tccgtcctaa caaggctgat
     1441 acttcagatc cagcttgagt gaaacggaaa atgttgtcga taaacaacag tacatcttga
     1501 ccttctacat cacggaaata ttccgcaatc gtcaaaccag tcaaagcaac acgcatacgt
     1561 gcacctggtg gttcgttcat ttgtccgaac accatggctg ttttttcgat aactcctgaa
     1621 tctttcattt cataatacaa gtcattacct tcacgagtac gttcacctac accggtaaat
     1681 acagaaatac caccatgttc ttgggcgata ttatggatca gttcttggat cagtacggtt
     1741 ttaccaacac cggcaccacc gaatagtccg actttcccac cttttaaata tggggcaagc
     1801 aaatcgataa ctttgatccc tgtttccaaa atttctgtac ttgtacttaa ttcgtcaaag
     1861 gctggtgctt ttttatgaat ttcgcttctt tccgcatctt ctgggaaagg tgtttctaaa
     1921 tcgatcgtat ctcctagtac gttgaatacg cgacctaatg tttctttacc tacaggaaca
     1981 gagattgctt tgcctgtatc aatgacttcc attcctcttt gtaaaccatc tgttgattcc
     2041 atcgcaatgg tccggatcac tccgtcccct aattctaacg ctgcttcaag aacaactttt
     2101 gatttatttt cgtcattttt ataaacaact aatgcgttgt tgatatctgg taaggattgg
     2161 tctaaagaaa attccacgtc gacaacggga ccgattactt gaacaatctt gcctgaactc
     2221 atcttttttc ctccaatctc aatactattc tagtgccgaa gctccggcaa caatttcggt
     2281 aatttcttgg gtaatagccc cttgtcttgc acggttgtaa gaaaccgtca gctcatcaat
     2341 gatattagca gcattatcag ttgctgtttt cattgctgtc ataccagcag catgttctgc
     2401 tgttttggcg tcaacgatcg caccgtagat caaactttca gcatactgag gcaataactg
     2461 agacaagatt tcttcttttg aaggttcaaa aatatattct tgttcgaaag tagttgcttc
     2521 ttctggatct aagtctgaga ttggcagcat tttttctaca cgaaactgac tagttagcga
     2581 attgatatga tggttataac atacgtataa ttcatcgaat acttcgtttt ggtacatagt
     2641 tgttgccata tttacgattt tgcggacttc atcaaagctt ggctgatcag atagattgcg
     2701 cagctcatat gctagattga taccgcgtgc tttaaagaag tctgcaccag ttccgccaat
     2761 tgcgatcatc acgtaatcat cgggtgattg gtggtcttct tctaaaatag acatcgtttg
     2821 tttcaatatc gagctgttat atccaccgac taaaccgcca tctgcagtga tgacgatata
     2881 acccgttttc ttaacaggac ggctgagcaa catgctgttg taattgatat cccctctagg
     2941 attttctgag gcaatatcat ttaactgagt agcagtcaaa tgcgtaacga tttctcttac
     3001 ttttgttgca taaacctgaa acttctgtga agaagcttca gattttgtca acttagaagc
     3061 agacaccatc tgcattgccc gcgtgatttg gctcgttttt ttcgttgaag cgatacgtgt
     3121 cttgatttca tttaatgaag cacccattca tctcacctca ctatgcattt tgaatggatt
     3181 tcaatttatc ttctgtagag gaattatttc cttttgtagc taagaacatg tctttatatt
     3241 cctgaatcgc actgttcaac gcttcttctt ctggaagatc ttttgttgta cggattgttt
     3301 caaagatatc tgaatgattg gtatccagat attcgaataa ttcatgttcg aagtctaaaa
     3361 tactgtctac agggatacta tctaagaatc catgtgtcaa agcatacaaa atcactactt
     3421 gtttttcaac agctagcggt gcatgcaatt tttgtttcaa gatttctact gtacggcgtc
     3481 cacgatttag ttttgcttgt gttgctgcat ccaagtcaga accgaattgt gtaaatgctt
     3541 ctaattcacg gtaacttgct aagtctagac gtaaagtccc agcaactttt ttcattgctt
     3601 tgatttgcgc agaaccccct acacgagata cagatagtcc ggcatcaacc gctggacgag
     3661 tacctgcgta gaacaagtcg ctttctaaga agatttgtcc atcagtgatc gaaataacgt
     3721 ttgttgggat ataagcagag atatctccag cttgtgtttc aacaaatggt aaggcagtca
     3781 ttgaaccgcc acctaaatca tcacttaatt ttgctgcacg ttccaataag cgtgagtgta
     3841 agtagaaaac atcccctgga taagcttcac gacctggtgg acggcgaagc agcaaggaaa
     3901 gttcacgata agcaacagct tgttttgaaa gatcatcaaa aatgatcaag acatgtttcc
     3961 cgttgtacat gaactcttca cccattgcag taccagcata tggtgcgata taaagcaacg
     4021 gcgctggttg agacgcaccg gcattcacaa cgattgtgta atccattgcg ccatattttt
     4081 tcaatgtttc aacttgtgta cgaactgtag aatctttttg tccgattgct acatagatac
     4141 agatcatatc ttgacctttt tggttgatga tcgtatcgat cgcaatggaa gttttccctg
     4201 ttttacggtc accgataact aattcacgtt ggccgcgtcc gattggtaca agagcgtcga
     4261 ttgcttttag acctgtttgc attggttcgt taacagattt acgttgcata acgcctggtg
     4321 ccattgcttc tactggacga gctttgtcag taacgatttc acctagtccg tcaattggct
     4381 gacctagcgg attaactacc cgtccaatca aggcctctcc aactggaact tccatgatct
     4441 ttcctgttcg ttttacttta tctccttcac gaatggactc gaaatcgcca aggataatga
     4501 tacctacatc atttgattct aagttttgcg ccattccgta tgagccattt gaaaattcaa
     4561 gcaactcacc actcatcgcg ttttctaaac catgtgcacg agcgatcccg tcacctacat
     4621 aagttacggt accgatttct tcgactgaaa gcacgttttg ataattttca atttgttctt
     4681 taatcaaggc actaatttct tctgctttga tggccattcg attcacctac ctctttgtac
     4741 tatctattta attgattacg catattttct aattgcgtgc gaatgcttcc gtcgattact
     4801 cgatggttag cttcaacaat cgcgccacca ataatagaag aatcaacgat ttgttttaat
     4861 tcgactgatt gatagttcat tgtttttgca acattctttt ctaattgtat tcgttgttcg
     4921 tctgaaagtg gaactgctgt tgtgacactt cccaacagca aaccttgatg ttcatcatat
     4981 cgacgttcat attcttcgat catgaacaaa agatcatcca ttcggttata cgcataaaca
     5041 acttctaaaa agtttttaac gattccatca tagccactaa caagcttgtc catgatttct
     5101 cgtttttcat gaggttctag acgaacatca cttaacatat tacctaatcc aggtacttgt
     5161 tcatagattt gacgtaagct tacgagatct tgataaattt cttctgcttt atttgaatcg
     5221 atcgccagtt caaataaagc tttcccgtaa cgtcttccta ctgtatattt atctagtttc
     5281 atttgaagaa cctagacctt cgatatattg attgattaat gattcgtgca tttctggaga
     5341 taattctttg ttcaagattt tttccgcgat ttgaagagat agttctgcaa catcgtcttt
     5401 tacagagttc agcgctgaat cacgttctac agtgatatca gcttgggctt tactttttag
     5461 acgagctgct tcttcttgag catccttcaa aatattttgg cggcttaatt ctccactttc
     5521 tttcgcattt ttgatgatat cagctgcatc agaacgagag gctaacaatt gttgttcgcg
     5581 ttcttgttcc attttcgctg agttgatgcg agattgttct gcagaatcca aatcattggc
     5641 gatcttgtct tcacgttttt tcaaaatatc gctgattggt ccccaagcaa aatgcttcaa
     5701 gagagccagt agtatcaaaa atgagccgct gacaacgatg acatttccta acatcgcatt
     5761 gccaacttct gcaattgcca attgattcag cataggcgag ccttcctttc tttgaagtac
     5821 ttctagtttt catgtaaaaa agcagtaaca aaccaaattg tttctgcctt aatactgatt
     5881 caattaaaca ccaaaaacta gaatcaaggc gacaactacc cctaagatag gaacagcttc
     5941 gactagagct accccgataa acattgttgt acggaattga ccagacattt caggttggcg
     6001 agccattgat tcgattgttt tagagataac ttgaccgtta ccataaccgg ctccgattgc
     6061 tgctcccatg attgcgattg ctgctgcgat ataattcata attgttttcc tcctaaatta
     6121 gataaatagt agtttgtttt taaaacgaat attattcttc cacttcaatc ttatggctca
     6181 tataaaccat agataaagta acgaagacaa atgcttggat gcttccgata aacaaggaga
     6241 atgcgatcca gaccatctct aatggaattg ctaacggcaa tgagaaccag cctaagctta
     6301 ttaccatttt tgcaatcaag gttagcaaaa cttcccccgc ataaatattc ccataaagac
     6361 gtaaagcaag tgttaataag tttgtaaatt cttcaatcag tttcatcgga aacataaaac
     6421 tatatggttt caagaacgaa tttacgaaat atcctttgaa gcctaacttt ttcaccccaa
     6481 ataagtgagt aagtaagatc attataaatg ccaaagttaa agtaacaaac gggtctgctg
     6541 tcgggctttt ccaaagtgtt gtttcgttag gtaaaacgat ttttgttact aaaccgatgt
     6601 tgtttgcaac aaaaacaaat aaaaacaatg taaatgccaa caaatggaaa ttgctcaatt
     6661 cttttctagg cagattatct gttacgattc ctcgactaaa atcaatgacc cattctatcg
     6721 cattttgttt gcctgttggt ttcaacttaa gatttcttgt aaaaaagtaa gctaagaaga
     6781 aaacaatcaa acaggtcagt aacaccatta tacaaactgt accatcaaac caaattggtc
     6841 caatgtggaa cgttagcgag cgttcatcca atccatttca cctcttttcc ccgaaattca
     6901 cataaaatca attcatgtgc gtatctggtt ttttccaatc ggtttctaac cacacggtat
     6961 cataccacca tgaaacagaa aattcaaaac attttcatga aaattagaca cttttttatt
     7021 tcagtcaaaa acgaagcgaa tttttcttta tacacggtgc tcctgatcta agaaaaaacc
     7081 tatgataatc atgttttatc agattaaatg ctaacaaatt ttatcattaa gtaaaagcaa
     7141 aagcaccgaa tatgcgaaaa aggataatga aattatctag agaaattttt taatgttcga
     7201 tcaatttgtc gatcgatatc ttttcgtttc aaatcttccc gtttatcata agattttttc
     7261 cctttcgcca gaccaatcaa taccttggca tagccgttac gaatatagac ttttaaagga
     7321 acaacagtga ttccagtatt tttcaattca ttttcaagcc ggatgatttg ttttttgtgt
     7381 aataataact ttctcgtgcg caacggatca tgattaaaaa tatttccttg ttcataagga
     7441 ctgatatgaa cattatgcaa gaaagcttct ccgttgcgga cgcgaataaa tccatctttt
     7501 agattgatcc ggctgtttcg tatcgacttg atctcggttc cttgcaagac catccctgct
     7561 tccatcgtgt cgatgatcga ataatcatgg cgagctttct tgttttgtgc aattaatttt
     7621 ccctcgcctt ttggcatagc tgtttcctcc aactcatttt attttttctt tttcttcttt
     7681 ttaacacctt tgtaaaatgg cttcttccct tttttcttac cgaacgtttg cttactatcg
     7741 ctatatcgtt tgttcttctt gccatttctt tctgggcgtt ttcctgcttg ttttccttcg
     7801 cgttttcctg attttccatt tcgtgttttt ggccgttcca acgctgtaac ctcttctgcg
     7861 gaaacaagtt caaaatctac tgctcgttca tctggatcag ctttgactac ttttactcgc
     7921 actttctgtc cgattttaaa agtcagacca gttctttcac ctacaagagc taaatgattt
     7981 tcaacaaaat ggaagtagtc ctgtttcaat tcattgatat ggatcaatcc ctctactgta
     8041 ttcggcagtt cgataaacag gccgaatttc acaacagagc tgatgatccc atcgaattct
     8101 tcccccactt tgtccatcat gaattctgct ttcttcatgg aatctacttc acgttctgtt
     8161 tctactgcac gacgttccat cttagaacta tgatctgcaa tttcaggaag ttccgtagcc
     8221 cacgaacttt gattgctttc acttttatct tcactgtaag aacggatcag acgatggacg
     8281 attagatcgg gatagcgtcg aataggagat gtaaagtgtg tgtagtaatc tgccgctaag
     8341 ccataatgcc caaaattgtc ttctgaatag cgtgcttgtt gcatgcttct tagcaacata
     8401 gtattgataa ctgcagcttc tggtttgtct tctactcctt cgatcacatg ctgtaaatct
     8461 tttggtgtga tcgttccttt cgttcctcgg accagaatgc ccaaagctga agcaaaatca
     8521 aagaagcgct gcattttttc ttcttttggc tgttcatgga ttcggtaaat aaacggcaga
     8581 tttaatttat tataatgttc agctaccgtt tcattggccg caagcatgaa agattcgatc
     8641 aagcgttcac caattcctct gcttcttagc tggatatcat ttgggtgtcc ttcagcatca
     8701 acaaggattt ttgcttcgcg atcctcaaaa gaaatcgcgc cacgtttgat tcgcatcgtt
     8761 tcaaggattc gatgcaattc acccatttct tggaacatag gtacgagctg agcatagcgt
     8821 tgcatcgttt cttcgtcttg atcttctaag atttgattga cagctgtgta ggtcatccgt
     8881 tctgttgttt ggatcacact tgggaaaata tcatgagaga ctacttgtcc atttggatca
     8941 atctccattt cgcaactcat ggtcagcctt ggaacatggg gattcagtga acagatacca
     9001 ttcgataaac gttgcgggat catcggaatc actcgatcag tcaaatatac acttgtccct
     9061 ctttcatatg cttctctgtc caattgactg ccttgtgtca catagtaaga aacatctgca
     9121 atgtgtactc caagaaaata attaccgtta tctaattttt taaccgtaac tgcatcatct
     9181 agatcttttg catcttctcc atcaatcgta acaatgagct gatttcttaa atctctacgt
     9241 ccagttatat ccttttcgct gatttgatct ggaacttcat ctgcttcaga caatacatca
     9301 tcaggaaatt tcgtagggat accattagcc acgacaattg agaggatatc catgccaggg
     9361 tcattcttat gaccaatcac ttgcttgatt atcccttcta aactagtcgc ataccctttt
     9421 tcagggtaat gggtcagttc gatcagcaca atacttccgt caactggacg aactccctct
     9481 gctgctgcat atactttaaa ttggttgagc tttctatctt tcgggataac aacgccgtaa
     9541 agatccgttt cttgtatttc ttcttctgaa taagcaacga attcccctgc taactgagta
     9601 gtcgcccggt ctcgaatctc gactactttc ccctctgctc cgcgatccat atctgtattt
     9661 gctcgcttca tgatatcgat tgctacggtg tctccatcca ttgcaaaacc agtcgcttct
     9721 tttgggatat agatgtcatc ttcttcggga tcaatcgtta caaatccaaa tcctcgttca
     9781 tttgctcgaa atgttccttc gatcaagatt ggttttaaag gaagtttcac ttttcctttt
     9841 ttattgaaaa ctatactctg ttctctttcc atttgagcaa tcgtttgtac tagtaattta
     9901 aaatcatcgc ttttttggaa ccctaaacct tcagcgattt cttccataga aaaactgcgt
     9961 ttgcgactgc tttccatgaa aaagagaatt ttctctttta tcgtttcttt tgtcatattt
    10021 attcctcatt ccaaggtaat ttttctaaaa attccaagac gtctttttct aattcgcgtc
    10081 ttgctgtacc aactgtaatc acatgaccac tttcaggata ccatcgaagt gtcactcgct
    10141 gacggctcaa cttgcgtgca gtttcaaata cgccattagg gtcgatcatt tcatctttcc
    10201 cagcttgagc taaaaatatc ggctgttgga tatctgacaa cctactttct acgattgctg
    10261 cctgatcttg tatatccatt aattgttgtt cagctaacga gcggtaagca actgcttttt
    10321 cattgatttc tttctctgat ttacctgctg tcttcagcac ttgacgtacg tatttttcaa
    10381 agttttcagg cacgtttgtt tttacaggag acaaaggcga acagaaaaaa ccgccgccaa
    10441 tcactgattc ttcttctagt gctcgtacag caaagatgcc gcccattgat aatcctaata
    10501 cggcaatatc agaaaactgc tcagactgta aaaaatggat ggctttttta gtgtctgccc
    10561 accagctttc tgctttttct tgcaaaatat catatggctc cattgttccg tgtcctttaa
    10621 aaagcggcgc ataaactgta taatctgctt tttccaaaaa tcgtgcgagc attcgaacat
    10681 cgtttggact tcctgagtaa gcgtgaagca gcaaaaccgc tcgctttcca tgttttgcat
    10741 aaaggggttt tggtaattgt ctcactcgtc tcacctcttt cgcctcattt taccatagtt
    10801 tctttcataa cttcttctta agcgttctct ttgcaataaa aaaagccgcc cgccgttttg
    10861 cgagaggctt attcaactac ttcgaagata aatatgctag gacaaataat aagatcatcc
    10921 agatagctcc cataactgca gttgatcgct gcatgacagc ttcaaagccg cgagcttttt
    10981 gtttgccgaa tagttgatcc gcaccgccgg taaatgcact cgctgcacta ttttgcttgc
    11041 ttggctgcat cataatcgca atgaccataa caatcgataa aacgatcacg attcccaaaa
    11101 ttaaattata catatcgcag cctccttccc aaaagatatc gtctctttaa ctttatcata
    11161 gataaaagtc cgtgacaact ctatataaat ggaataacag ctgaaagttc aaaactttca
    11221 gctgttattc ttatgactga cgtaagacgt cttgatattc tggtttcttt tcaaactctt
    11281 tttttgcata agggcataaa ggaatgatct ttttcccgtc tcgtcttgct aattctactc
    11341 cagtatacac tagcttttca gctaactttt gaccacgata tgctggatct acaaatgtat
    11401 ggtcaatgat catgatgtct gggcccgcat ctgaccatgt catttctccg atctcttgtt
    11461 gttcatcatt gaataaagca agacgatttt tttcttcttt gatttccata tgccgcactc
    11521 ctcaaataac tgatattttt ttattcctac cttattttag cagtctcctg atctataaac
    11581 aattatttat cctcgaatta cttacaaaaa agaagagaaa attaattcat cgttattctt
    11641 tgttcaataa atgaaaaaat cactgtccaa taggttttcg gatctttcat cgatgcttcc
    11701 aagtgttctg cttctggaac aagcagcagt gctttatccc ctgccgtagc attttgaatc
    11761 gtgtacgctg cttctgtagg gacaaaatga tctctttcac catgaatgac caataaaggc
    11821 agatggttgc tgccaagctg tctcgtagct gatgcttgct ttaaggagta accggcgtat
    11881 ttctgggcat aatgattcgc atatctcatg accatttttt taggaaagac agtgatttta
    11941 cttaacatat atcggaattc tgcataaaca gaaacatatc cgctgtctaa aataaatcct
    12001 ttgaccgctg atggaagttt ttcaccactt gccatcatga tcgtagaagc tcccattgat
    12061 ccgccgtgta aaataatgga cgcattcggc tgttcatcta agatgagttg gatccaggca
    12121 attaaatcta gccgatctag ccatcccatt ccgatgattt ctccttcact ttctccgtgc
    12181 gcacgtaaat caggtacaag aacgttgaat cctttctctg catatctttt acctatatga
    12241 gacatgtctc ttttccctgt gcttcggtaa tcatgtacgc aaatgaccca tttattctgc
    12301 ttatttggat ggatgaacat ctgaccagct agtttcaacc cgtcataact tttgattgtt
    12361 ttgggtgttc cctgctccca aaagctttca cactcttctt ctaatttttg tcgttttttt
    12421 tctactgacg tgactgcagc agggtccatc aaacgatgac cagtgtcttc ataccagtca
    12481 tatcctctct gtactcccgc tcttactaat ttctttgcat aaaaataaga aagaaaatgg
    12541 taactgatcc ctataattat cagaataaga atgaaataaa aaataaccat tgtacacccg
    12601 ccgctttctg ccaaactata atctaactat tatccaatgt accgatccat aggattcttt
    12661 attttcggca cacgtaaatt ccatttcttt ttcattgtat catttactta aagttctcct
    12721 caatagattt acttgtaaga gagcatttac attcgcaata aaagccagta tactaatttc
    12781 gtgcattata atcaagacgc tcgtcttgga ctggttcgga aacttcccag aataaaaaac
    12841 taagtatctc taaagaaagg agattggaaa taatgcgaaa agtaattatt gattgcgatc
    12901 cagggatcga cgacacttta gctttatcat tagctgtcaa atcccctgat atcgaagtag
    12961 ttgctattac cgtagtttgt gggaatgtcc ctgcagatat aggtacgcag aatgtgctga
    13021 aatgcctgga acgctgtgat cgtttagaca ttcctgttta tcaaggaatg gaaaaacctt
    13081 tgaagcaacc tttcatcagt gcacaagaca ctcatggtat ggatggttta ggggaaacaa
    13141 atttcccgat gatcttgaga aaacaggcag aacctctcca tgccgttgat tttttggctg
    13201 attatttcaa agaaaagaca aatacatcag tcattgcttt gggtcctttg accaatatcg
    13261 cttttgcttt gaaagtcaac ccaaatattg ggaaacatat ggatcgtttt gtctcgatgg
    13321 gtggaactta caaaagccat ggtaactgtt cgccagttgc tgaatacaat tactggtgtg
    13381 atcctgaagc agcttcttat gtattcgaga atctcaaaca aacgatagaa atggttgggt
    13441 tagatgtgac aagagaaatc gtcttgacac cgactatcct cgaatactgc tgtcaaatga
    13501 atcctgaaga gggagagtat ctaaaagcga tcactcgttt ttattttgac ttccactgga
    13561 aacaagaaag aattttagga tgtgtcatca atgatccgtt agctgttgct tattttatcg
    13621 agggggccat ctgtgaaggt ttcaaaagct tcactgctgt ggaaactcaa ggaataagtc
    13681 gtggacaaac attggttgat cgttatgaat tttggcagaa acctgctaac agtaaaatta
    13741 tgacaaaagt agatactcgt ctttttttcc gaaagttttt aaccgttctt ttaaatgcac
    13801 aagaagaaac gattatgaaa gatttggaaa ggttaaagat gggctaacaa aatgaataaa
    13861 aacaaaatgg caactcgcgt acttacagtg atggctttgt gtattgggat caattatatt
    13921 ggaggaacga ttgctttatg gcttcgtttg ccaatctatc tcgattctat cggaacgatt
    13981 tttgctggtg cattgcttgg gcctgtacct ggaatgctga caggattgtc cagcggtatt
    14041 ttaagtggag tgactacaga tattttttct ctgtattatt cgccaatcca aattctcaca
    14101 ggcttgcttg ctggattgat tttgccaaga tatctcggca aagataaaaa cagcttgaaa
    14161 ctgttattac tcgctttgat tttgtcggta cctggaacga tcgtttcttc gatcattacc
    14221 gttcagttat ttggcgggat cacctcctca ggatccagta tgatcgttca gctattacat
    14281 gggttaggtt taagtcagac aacaagtgtg atcttggtac aggcaggtac agactacctt
    14341 gatcgtttgt tgtctatcgt cgttgtttca ttagtcatca ctatgttgcc acaacgcttg
    14401 atatattcaa aaagaaaaat aggctgaata aacagagctg gattagcaaa ataatgctaa
    14461 tctggctctg ttttttcatt tattaaaaaa atcttacgct aagtaggata aaataattcc
    14521 ttgatatttt tcaatcatat ccaaataatt atcgatttca acatactcat ctacttgatg
    14581 aggcaaagta acgacaccag gcccaaagac gacaaagtcg aaggaatggt tagctttcgt
    14641 aaattcggct gcatccgttg ttgctgccgc tccaactaaa ggtaatggct ggctgaattg
    14701 ttgctgaatg caatgaatca aaggagaatc tggatcagct ttgacaggta ttttgttgta
    14761 atcgatcatc aattccaaat gatagtctgt ctcttgattc aattcattga cgattgattg
    14821 cagcaatgca atgattttat catttgggta ttctggaatc gatcgaatgt tcccttggag
    14881 ctgcgcatgg cttgggatgc tgttgacttg attcccgcca ctgatcaatg taacattatg
    14941 gattgttttt cctaggacag gattctcgat tgtttcagcg aggtgattca tttcagcatt
    15001 tgctttcgta atgaattcat ttaggtgatt gattgcatta taaccttggt ctggcataga
    15061 gctatgcgct tcttttccgt gcgaggtgac tgtatagttg attgatccca tgtgcgtata
    15121 catcaaactg taattagttg gctctccgat gatcaaggca tctaagtcat ctacatagcc
    15181 tgctttcgtg agctgttccc cacctagttc acccacttct tctccgacag tggctaataa
    15241 tttgactgtt ccgttaaatg gttttcctga ctctttcagt tcgatcatgg caatcaccat
    15301 tgctgccaag ccactcttca tatctgtagc accacgtccg tacaaacggt tcccatggat
    15361 ttctgctgca aagggtgcat aggtccaaga cgattcatca ccagccgcca cgacatccat
    15421 atgcccagat aggcctaaca cttttcctga ttggcccttt tggtaacgag caatcaaatt
    15481 atctctacca tctctgtaag aaacaatctc tcctgttatg tcatgctttg ccaaaagctt
    15541 attcaaatag gctgctactt caccttcgtt tccattcact gatttaattc ggatgatctc
    15601 ttgtaaaatc gcgatttttt cttctttttt catctaatcc tctcccttgc catttctttt
    15661 tattgtattt gagtgtgctc gaattttcaa ataggagcaa ccaaagtaaa aatgaagaaa
    15721 gagagccact ttatgaaaaa tatgctgcta cttccctcct attttattta tacgagagga
    15781 cttgctcata gattcctatt ctactgttct atccttaaaa gctcttatat gatgctagtt
    15841 attgttagct ttcctttttc aaggatgatc ttttcttgta ccattctttg taaacatcat
    15901 atacagaagc tagttgcaaa aaaaactctc tacctgctga tattt
//
LOCUS       DAZUMB010000059        15857 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000059 DAZUMB010000000
VERSION     DAZUMB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.060"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(416..782)
                     /gene="ssrA"
                     /locus_tag="ACG80L_001633"
     tmRNA           complement(416..782)
                     /gene="ssrA"
                     /locus_tag="ACG80L_001633"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(953..1726)
                     /locus_tag="ACG80L_001634"
     CDS             complement(953..1726)
                     /locus_tag="ACG80L_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5023864.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1870..2220
                     /locus_tag="ACG80L_001635"
     CDS             1870..2220
                     /locus_tag="ACG80L_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5023865.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2201..2917
                     /locus_tag="ACG80L_001636"
     CDS             2201..2917
                     /locus_tag="ACG80L_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5023866.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2917..4365
                     /locus_tag="ACG80L_001637"
     CDS             2917..4365
                     /locus_tag="ACG80L_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5023867.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4435..4944)
                     /locus_tag="ACG80L_001638"
     CDS             complement(4435..4944)
                     /locus_tag="ACG80L_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023868.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4934..5659)
                     /locus_tag="ACG80L_001639"
     CDS             complement(4934..5659)
                     /locus_tag="ACG80L_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023869.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5882..8002)
                     /locus_tag="ACG80L_001640"
     CDS             complement(5882..8002)
                     /locus_tag="ACG80L_001640"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5023870.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8232..9410
                     /locus_tag="ACG80L_001641"
     CDS             8232..9410
                     /locus_tag="ACG80L_001641"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS5023871.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9426..10184
                     /gene="aroD"
                     /locus_tag="ACG80L_001642"
     CDS             9426..10184
                     /gene="aroD"
                     /locus_tag="ACG80L_001642"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS5023872.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10207..10692
                     /gene="ybaK"
                     /locus_tag="ACG80L_001643"
     CDS             10207..10692
                     /gene="ybaK"
                     /locus_tag="ACG80L_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS5023873.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10763..12016)
                     /locus_tag="ACG80L_001644"
     CDS             complement(10763..12016)
                     /locus_tag="ACG80L_001644"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5023874.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12134..13483
                     /gene="gdhA"
                     /locus_tag="ACG80L_001645"
     CDS             12134..13483
                     /gene="gdhA"
                     /locus_tag="ACG80L_001645"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS5023875.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13595..14941
                     /locus_tag="ACG80L_001646"
     CDS             13595..14941
                     /locus_tag="ACG80L_001646"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS5023876.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15249..15635)
                     /locus_tag="ACG80L_001647"
     CDS             complement(15249..15635)
                     /locus_tag="ACG80L_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5023877.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15684..>15857)
                     /locus_tag="ACG80L_001648"
     CDS             complement(15684..>15857)
                     /locus_tag="ACG80L_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS5023878.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         5181 a         2827 c         3184 g         4665 t
ORIGIN      
        1 tacaggaatt attacttctt acaaatgtat agcgaatgtg aagcctgctg ttttaattgt
       61 tctagaaaaa cttttgagtg aacaattatt ttatgttagg atgaattaat ttctctagag
      121 tttataatta aaaaacttat ttgaaggaac atgcttaaac cataattgtg tttttccttc
      181 catcagcata aaagttcacc tcaaaaagag tgtacgaaca aaagttcttt ttgtaaacct
      241 taaaatagat tttcctgaat acgtattggg gatttctaaa tgtttgaatg tttttttgaa
      301 tgcaaaatca atgcaaaaaa ttttgtattt ctctactttt ctctccttga ccaaaataaa
      361 aaaacgttga tttaacaacg tttttcatac atgtttatct gtctcaacca tacatatatg
      421 gagacggcgg gagtcgaacc cgcgtccaaa cacatcgcca cttaaaaatc tacgttcata
      481 gtcaactcat ttaaggattc actttacagc ttgccgagtg acaggcattc tgtatcgcta
      541 gtctgatgtt ctctttttca acttacagac ggaaagttga aacgtatccc actaaagtta
      601 ggacccttac tcgagcacat gggcgatgcc gagaggatct acgctaactg tttttaggca
      661 gctaaagcgt aagagttgtt ttcgttttta gcagttatat ttaactgtaa cgtttttacg
      721 tagacgtaac ctacgaaacg caattcaagc tcgactgtgc ctgtcgaatc cgtaacgtcc
      781 ccgttagcta acaagggtta cgtgtatttt tcatacaaaa ttagtatagc atatattggg
      841 ataaattcaa taaaaaagcg aatcatatcc cattatttat ataataactg cccctttctt
      901 aagaaaaaat aagcgatttt cttaagaaaa gaggtagctt caaaaaaaga tcttaaatca
      961 gatcaaaata cttcaatgcg tggacaattc cgccttctgt atttttcttt gtgataaagg
     1021 tagaaagttc ttttaatccg tcataggcgt tgcccatagc gattttgttg tcgcaagctt
     1081 ccaacaaggc aaaatcattc ggaccatcgc caaatccaaa agtaggaact ttttccagct
     1141 gaagttcttt ttgcagacgt ttgacacctg ccccttttga gattcctttc ttcactgtat
     1201 cgatcgagta aggaccattc cgataaaaag ttagctcagg aaaatgttcg tgatagtatt
     1261 cgtcacctga ttgagataag atcagcaaca tatttactgt gttttcttta aagcctaaag
     1321 gatcaatttc cggaacatct gaatgaatga agtcgtaagc attgatcatt gtttggctat
     1381 gaccggtaca ccagattttc tgatcgttat agtaagaaag ttcatgaccg ttcaactgta
     1441 catgatcata cattttttga cattcttctg aagaaaatgt ttgagaatag atttcttcgc
     1501 cgtctactcg aatgaaagaa ccattcatgg tgaccgcaga agtaatgccc gcatcttcca
     1561 tgatagagtg gatttctgct tctgtacgtc ctgtagcgat gacggggaga acattgtttt
     1621 cttttaatgc gttcatagct tttgccactt cgggtgtgat ttttgatttc gcatctagta
     1681 atgttccatc aagatcaaag aaagtgattg cacgataatt tgacacaaat aaaactcctt
     1741 ttttattgga ataagtttat cttaacaaag aatgaacaaa agacaaacct ttcgtgaaga
     1801 attcctgaac atattggttc agtcaacaat ttagagtata ctataagaag agaatttagg
     1861 agggaaagaa tgattttttt cagtattggt atcttattct taataattgg cagtcttttt
     1921 cggattctgc cctcaaaagg aaatctgcct ttttatgggt atcattcgcc tcttgccgca
     1981 aaaacagacg ctcattggcg gctggctcaa aagacaagcg gaaactggtt ttttcttatg
     2041 ggcttactga tggcattgat cggctattat ttaaagacat ctgggcatac gaattatttt
     2101 ctgattgaaa tgcttctgct agtatttcca attatgccta tttttattat gacagaaaag
     2161 aaattacaga aatatgacct ggaaactgga gggaacgata atgaatattt taatgattga
     2221 agataatcaa tctgtttctg aaatgatgca gatgtttttc ttaaatgaag gttgggaagc
     2281 aactttcaag tatgatggaa aagaagggct cgatgccttt ttagaaaacc cacaacattg
     2341 ggatatgatt acactagacc tgaatttgcc cacactggac ggtatgctag taggaagaga
     2401 gatccgcaaa gtttcaagta ctgtgccgat tatcatgctg acagctagag attctgaaag
     2461 cgatcaggtg atcggcttag agatgggggc agatgattat gtcacaaaac ctttcagtcc
     2521 tctgacgctt attgctcgaa tgaaagcttt gcatcgccgt agtgagttgg cagaagcaag
     2581 agaagaagca gcagtttcag atgatcattt tgatatcgag actagtcatt tcaagatgaa
     2641 tacgaaaaca cgagaagcgt atctggacaa ccggccaatt gaaggactga cgcctaaaga
     2701 gttcgatcta ttgtttactt tagcaaaaaa accacgtcaa gtattctctc gggagcagtt
     2761 gcttgaatta gtctgggatt atcagtactt tggtgatgaa cggacagtgg acgcccatat
     2821 caagaaatta cgacaaaaaa tcgaaaaggt cggaccacaa gtcatccaaa ctgtttgggg
     2881 agtcggctac aaattcgatg attctggtgt tgcctaatgc gttatttgta tcagcaactc
     2941 ttagcttttt gcggaatggt cgcgttgatc ttattgatcg ttggagtttc ttttacacaa
     3001 ctgacaaaac aaacgattga agaaaacaat taccagcaac tgtttggata tgctgaatca
     3061 gttgaaaaga caacgcaaac ctatgccgat acgctcccgc agtttagtca agatcaggcg
     3121 tttcaaaatg cattgctgct gacagagcaa gtactgacag aacaaaatgt gaactttata
     3181 tttatcgata aaaacgaacg tgtcatttat ccgactaata cagctttact gaattttacc
     3241 ttaacagctg atcaatggaa aaatctgagg aatggacgtc aagaaaaatt cacatcgaat
     3301 aaaaatattt taggacaagc acaggcgacc tcttacgcac ttgtgccatt taatttgaat
     3361 catgaattct atggagcact tgtagttagt caacctgcgc gaaatatcga taacagcgtt
     3421 cgtccaatta cactgaacct gttcaaagga tttatttttt ccagtatcgt tgcagttatt
     3481 gccagctatg cttttgcagc attccaagtt aagcgtatca atcggttaag gaatgcgacg
     3541 aaagaagtga cgaatggtaa ttttgatgta cagctccccg tacatgacaa agatgaattt
     3601 gatgagttag cagatgattt caataaaatg acgaattcat tgaaagaatc tcaagcagaa
     3661 attgaagaac aagaaaatcg tcgaagacaa tttatggcag atgcttctca cgagatgaga
     3721 acacctctta cgacaatcaa cggacttctg gaagggttag aatacaatgc aatccctgaa
     3781 aatcagcgag aaaacgcgat taaattgatg aaaaatgaaa cagagcgtct gatccgtttg
     3841 gtgaatgaaa acttggatta tgaaaaaatc cgtacgaatc aaatcagtat ggtcattaaa
     3901 aaattcaatg gaacagaaac attgaaaaat attgttgcac agttagaagc taaagcagaa
     3961 gcagcaggag atacattgac attgaaagcc gttgatgata ttgatgttta cgctgattat
     4021 gaccgatttg tccaaatcat ggtcaatatt attcaaaacg cgatacagtt tactgaaaac
     4081 ggccaaatca cgattacttt agaaaaaggc tatcttgaaa cgatcatcac gattgaagat
     4141 acgggaattg gaatgtctga acaacaaatg aaaagtatct gggatcgtta ttataaagta
     4201 gatccttcaa gaaaaaatac aaaatatggt gaatcaggat taggtttacc aatcgtgcag
     4261 caacttgtac gcttgcataa aggaaaatta gaagtagaaa gcgaactagg aaaaggaaca
     4321 aaattcgctg ttcgtttgcc tgacgtagaa atcaaaaaag agtaaaaaac aagcggtata
     4381 aaagatcatg aatcgatctt ctataccgct tgtttttact ataaaatgat tgatttagcc
     4441 cgcgggctct aaacgatcaa tataaggact ttgagcaata gtagcatcat gagtaaagag
     4501 taagatacct tgattttctt catgagctaa ataagaaagc aagtggagta gctcttggat
     4561 ctccaataca ctaagttcat caaatgtatc ttcaataaca atattgtttc gcttggaaag
     4621 caaggcatga agtaattgca gctttacttt ttcaaaagac gtcaactcag ctgccgcaca
     4681 attaagcaag gaaggagaga tatcaagttt ttcttgatag gccaaacgat cttgtttgat
     4741 ttctttttcc ggtacatcca acaggacatg gtctttaaga ttcagataag gaaacatcgg
     4801 ccaatttttt tggatgatcg caaaattatc cagttttgca acaagaacct cttttttcaa
     4861 gtgctccaat attttatgct gtttttcgag aggacacaaa ataacggtca agtgttcagc
     4921 atgaatcaaa tattcaggtt tcattacgtc caggcctcct actttgcaaa tgatggataa
     4981 aaaaagtgaa agggaataga ctaagaatga gacagcatcc actgagccca ataagagttt
     5041 gggcaaatcc cttaagtata tctgtgaaaa aaagatcatt ttggaaatcg atggtaaaaa
     5101 aagtctgttg atccatcgga attgtaagtc cgcttaccga agtcaagata tcacggatat
     5161 ctggcagttc agtagtcttt aagacaagaa aattgatcct ttgaagaagc gcttcataaa
     5221 ctgactggaa gcttacgatc cacaataaaa ccaagataca acaaagaaag agaggaaaga
     5281 acagctgcca gaaaataaaa aggaataatt tggttttaga aagaccagtc aggcgccaat
     5341 tgatcaattc ctgcttgttt tgcctgactg aatagtaaaa gaaaaagata aaaaggaagg
     5401 caaatagaaa aaataaactg aatatgcctt ttatataaaa tcggctagct aattctaaag
     5461 ccggaagaag ctctgtagat gcagaagcgc caataaatga aagccgatct tggacttgat
     5521 tggctaaagc cgattgcaaa tggtaaagag tcaggcatgc tatcaataaa aaagcagaaa
     5581 agcttgaaaa taaacaatag agtattgtct gctttttgta ataaattaat cgagcagtag
     5641 catagcggat tattttcata cttttcacct ctataaaaag tatagaaaat gaatatgaat
     5701 aaagtatgaa attcctttag tgaaatcacg aattaaaacc agaaaatatt ttgtaaaaag
     5761 caagaggttg tgaagaagct gccctattct attaccgtag atgcaagctt ttgaacactg
     5821 tttattcgaa aagaggagca gcgacaataa ttttgtcgct gctcctttta aatttatttt
     5881 gtcattcgga agaacttgtg gtcgttccgt gaagttcttg gtacgtttgc gttttataaa
     5941 gatcaacagt tgattgtccg ttgtgttcac taaagacact agttgacttg tcaccaagag
     6001 atttttcagc ttcttttaat ttctctaagc cattcttgta gtcataatca ctggtatcaa
     6061 ttgggtctaa tccactattc gtatagaacc gaagaagatc accattattg atttggtcag
     6121 aaacagctaa ctgtttatca actttttctt tcagtgaatc aatttcattc tgtacttcct
     6181 cagtaggttc agtaatagct tcaccagttt cgttactata gatcgtacca ccgtagtaag
     6241 tatattttgg tgtaacaaaa tcaccatcac ggaaagaaac aagctgttca tgatctttcg
     6301 agaatagatc ttgtcctaac tgaatataat tttttgtatc tacacctagt aagtgaagta
     6361 atgtaggcaa ggcatcaact tgtccgccgt aagtgtgatt gattccgccc tgtgtttgtc
     6421 cagggatatg gatcatataa gggacacgct gcatttgtgc attgtcgtag tttgtccaag
     6481 tagaacttgt ttttccaact aattcagcta gatttttatt tcttgaattc gagacaccgt
     6541 aatggtcacc gtaaaggacg atgactgaat tatcatacaa gccgctttct ttcaaataat
     6601 tgaagaattc ttgtacggcg gtatctaaat aattggctgt cgcaaagtaa ccgttgatcg
     6661 tttcatcatt tgtattggct aacgggaatc ctgcttcgtc attggtgaat tttgaataag
     6721 gataatggtt agatacagca atgaattttg aataaaatgg ttgttgcaga cgttccaagt
     6781 attgtacgga ttgttggaaa aatggtttat catgcaaccc gtattggaac gaattgtcat
     6841 cattaacatc ataataagaa gaatcaaaga aataatcata tcctaaattc ttgtatgtct
     6901 cgtttcgatt ccagaatgta ccagcgttcc catggaatac cgcactagtg taaccttgtg
     6961 tttgacttaa aatatcaggt gcagcttgga aagtattttt tccgcctaat tgtgtgaaca
     7021 aggagccttg attcaagcca aacaatgaat tttccatcag cgtttccgca tcgcttgttt
     7081 ttcctgcttt tacttgatgg aaaaagttgt caaaactgaa cgtgctgttg ctgtggtaaa
     7141 gactattgat gaaaggtgta acttcatgct ggagaccatt ctcatctgtc aatttataat
     7201 cgatcaggaa ttgctggaag ctttctaaat ggatataaat cacatttttt cctttagcga
     7261 gcccgaacaa atcgtcattt ggtgctgcat aatgttcttt tacataattt tctacttctt
     7321 tcatatcatt gggacttgct tctgcccgca cttggtttgt ctggtaagtt tgaacaccgt
     7381 cataaaccat gaaagcattc aagccaagat atttgaccaa ataatcacga gagaatgttc
     7441 gagtcagcaa ttctggacga tcagcttccg ccataaaaag atttcctgag aaaatcataa
     7501 ctgctaatgt agagatcgca aaagccattc ttgctcggat cgggcgaggg tccaacttga
     7561 ttttttttgc aatcagcaga acagtcaaga taataaaatc gatccaatag atcacatcat
     7621 atgggcgaaa cagtcgaatg gcactttctc ccagaccact cgctactttc ccagcaccta
     7681 aaatcgtgtt gatcgtaatg aagtccgtga attctcgata ataagtgaca ttcgaaaaaa
     7741 caagcaatgt caaaagaaaa taaatgacca tcatcgtgat ataagcaact ctagggcgtc
     7801 tgatataaag agcaattcca agtaataaca atgtagttgc aatagggttg ataaacaaga
     7861 taaagtattg aatgccactt tcgattccga gatggaaatc aactaaatag gcaaaaatcg
     7921 ttttcaacca aagtaaaacg actagtaaag aaaaaaatcc gagcctctta ttcaaaaagg
     7981 caggtgtttt tatacatttc aagataatct tcctttctgc acttcttaat cacagaagtg
     8041 tcatgaaacc atgttacaca gctcacttat tctcatttat tttactagta tgacaatata
     8101 gtgtcaattg aaaccagaag tttatgctgt ttcattaagg ttttctttaa gcttcatgaa
     8161 aaaatgagca gtaagacaag tcctatgttt tttgttatac ttgggaagag aaagctaaag
     8221 caggtgaagg aatgaaagta aaagtaaaaa aaagagcaag caatcgattg aaaaaaggat
     8281 atccactgat ccaaaaagaa gaccttgctc atccccaaaa agaaaccacg caatgggtgg
     8341 aattagttga ttcgcaaaac caatttatcg caaaaggata tctaggaaag cagaataagg
     8401 gaatcggttg ggtattgagc cagcagaaca tcccatttga tcaatctttt tttgaagaat
     8461 tttttgtgaa gtcaaaagaa aaaagaactt tctttttttc agatgattct actaccgctt
     8521 ttcgtatatt caatggagaa ggagacggaa tcggcggtct gattgttgat tattatgcag
     8581 gttatgctgt tttttcttgg tataacgaaa cgttatatgc acatcgtgaa ttattgattc
     8641 aagcttttcg tgaaatctac ccggaaatcg taggaggcta tgagaaaatt cgatttgaat
     8701 cagtaaatct gcctgagagc cagttcattt acggaaaaga ggcaccggaa cccttgatcg
     8761 taaaagaaaa cggagtaaaa tttgccgttt atttaaatga aggattaatg actgggatct
     8821 ttttagacca aaaagaagta cggggatcat tagcagaagg tttcgcttct gggaaaacag
     8881 ttttgaacat gtttagttat accggcgcct tttctgttgc tgccgcaatg ggaggggcaa
     8941 tcgaaacaac aagtgtcgat ctagcaaaac gcagtttaaa aaaaacacag gaacagtttg
     9001 aagtgaatgg tttggatgtc gatcagcaaa aaatcattgt tatggacgta ttcgagtatt
     9061 tcaaatacgc caaaagaaaa caatttttat atgacatgat tgtattggat ccaccaagtt
     9121 ttgccagaaa taagaaaaaa gtctttcgcg tagcaaaaga ttatggggaa ctggtcaagg
     9181 atagtctgga tattttagca gaagacggga tacttattgc gtctacaaat gctgcgaatg
     9241 tctcgataga acaattccaa acaatgattg aagaggaatt ccatgccaaa aatgtttctt
     9301 ttgaacaact tgctttatat cgtctgccaa aagatttcca gacgacggat acctttttag
     9361 aaggcaatta tctgaaagtg tttgtttatc aaattaaaaa taaccattag aaggagcgat
     9421 gcagaatggt ttgggtaaag ggattgaata taggaagcgg tagaccaaag atttgtgcgc
     9481 ctcttatcgg ggccgtccga gaggaagtat tacgtgaagc agttcttgca cgtgaagcag
     9541 gggccgattt agtcgaatgg cgagtcgatc attatcgtga agttttttct tttgatgaag
     9601 tagcagaaac attggttctt atccatgaga tgcttgatgg actgccgtta ttatttacgt
     9661 ttcgtacctt agctgaaggc ggagaacaag acatatcgat ccgtcagtat cgcgaccttt
     9721 atgaacatgt gatacacaca ggattagtag atatggtcga tatcgagttg ttcaaagtag
     9781 aaagtctagg aaaaagactg atagaggaaa tcaagtcttt aaacattcct ttaattatca
     9841 gtagtcatga ttttaaagaa acaccagcag accctgtctt gctttaccga ttgaatgtta
     9901 tggaacattt tggagccgat atcggaaaac tggcagtaac tcccaataat gaacgagatg
     9961 ttttgcggtt gatggagttg actagacggg caaatgcttt tgtctccatg cctattatta
    10021 cgatgtccat gggaaatctt ggtaaaattt ctcgtctggc tggcggaacg acgggttccg
    10081 ttatgacatt tggttcctta agcagcgatt ctacttctgc tccaggacaa ttaccggtca
    10141 aagcattgaa gcaaattatt caattattaa atgaaccgtt gtagaacaag tttgaggtga
    10201 tattttttgg caaagaaaaa aatagtaaaa acgaatgcaa tccgtatggt cgaacaaaaa
    10261 aagattccct atacagagca cgaatatgag tgggacgaaa gccacctaag tgcgtcatcg
    10321 gtcgctgaac agcttccaga aagccaatct cgtatattta aaacactggt tgctgtgggc
    10381 aatgtgacgg ggcctcttgt tgctgtgata ccgggagaag ccgaattgaa tctaaaaaaa
    10441 ttagcaaaag tttcagggaa taaaaaagta gaaatgctcc atttaaaaga tttggaagca
    10501 acgaccggat acatacgtgg tggctgctcg cctataggta tgaaaaaact atttcctact
    10561 tatttagatc aaattgcaga aagctatgaa caaatcattg tctcggctgg cagaagaggg
    10621 ctacagatgg agttagcacc tcaagatatc tgtgcattaa cgagcggaca atttgcagat
    10681 ataaaacaat aaatcatacg tataaaagaa agaaggaggc cgggatgttg tttgtccagt
    10741 ctccttcttt cttttttata cattatgctc tttgcccggc attcattcct gctacatgcc
    10801 ctgtagcgaa agccgctgtg atattaaagc cgccagtata accattgaca tccagtaatt
    10861 ctcccgcaaa atacagacca tcaatgattt tactctccaa tgttttagga taaatttcct
    10921 ttagagaaac ccctccgcca gttacaaatg atttctcaat aggaaaagtt ttgttgattg
    10981 atagcttaaa ctctttgcat agttgaacaa aatcttgaat ttgtttttga gaggcttgtt
    11041 gtcctgtcag atgatccatt tcaagccgct caagaaagaa aactaacaat cgctcaggca
    11101 atataccatg ccaagcattg accaagttct ttttggttgc tttacttttt tctgtcaata
    11161 tatgaataag ctcttgtttg gtttgtgtag ggaaacaatc cagagaaagt gtcactggtt
    11221 ctcccgtttt ctctaattct ttattgacaa aacttgaaca acgcagtgca gccggaccag
    11281 aaataccgaa atgggtgaaa agtaaatcca tcgtatgttc agtcaataca cgaccttttt
    11341 tatttaaaac gcgtaaagtg atgtcttgta aagaaagccc ttgaagtgtt ttctcctgaa
    11401 tatacggttc atccgatatc aagggggact ctgtcgcata aagaggcgtc accgtgtgac
    11461 ccactcgctt cacgatcttg tacccatctc cagtagaacc agtagatgga taagtccgtc
    11521 caccagttgt taagataatg gcaggagcat ggaactcttc gaattccgtg cgtataccgt
    11581 aaattttatg ttctttgtgg atcaatttct ctactcgagt agagaataga agcgtcacat
    11641 ctagttcttt cagacgattg agcaatgctt cgataatcgt ttttgattta tttgttacag
    11701 gaaacatccg tccatgatcc tcttctttta aatgtacgcc ttgtgattca aagaagttca
    11761 tgatatcgaa attattccat tgcgcaaacg tactatataa aaactttcca tttccaggaa
    11821 tatgtgcaat caaatcatca acaggacgat tgttcgtaac attgcaccga ccaccacctg
    11881 tcatcagtag tttttttcct gcttttttgt ttttttcgat caagagtacg cgtgcgcctt
    11941 gttcagctgc actgatcgct gccatcatcc cgcttgttcc agcgccgact acgatcacat
    12001 catacagttt ttccattccc tcacctcaag gaaagtgtag catatctgaa gaaagaaaac
    12061 agaaaaaaaa tgttttgagc caaaagagaa aatgagctaa aatgagggaa gataagcaaa
    12121 ggagcgtgga acgatgacaa acgcaacaga atatgttcaa gcaattcaag aaaagctaca
    12181 tcaaaaggat caagggcaag aagaattttt acaagcaatc gatgaattca tgcccacggt
    12241 tatgtctttt ttggacactc atcccgaata tatcgaaaag aatatcttag aacttttaac
    12301 ggaaccagaa agagttattc aatttcgagt gccttggcaa gatgatgaag gaaactggcg
    12361 agtaaataga gggtaccgta tccaatacaa ctcagctatc ggcccttata aaggtggatt
    12421 gcgcttccat cctagtgtca atctaagtat cttgaaattt ttggcttttg aacagatttt
    12481 caagaatagt ttaaccggtc tgccaatcgg tggaggaaaa ggcggaagtg acttcgatcc
    12541 taaaggaaaa tctgataatg agatcatgcg tttttgtcaa agtttcatgc tggaattgtc
    12601 aaaacatatc ggcccatcga ttgatgttcc tgcgggagat atcggtgtag gagctagaga
    12661 aatcggctat ttgttcggcg aatataaacg attgaaaaaa tatgatactg gtgtgttgac
    12721 aggtaaacct ttggattttt ggggaagtaa gatacgtacg gaagctactg gttatggatt
    12781 agtgtattat gtcaaacatc tattgaacga agaaaaagat tcatttaatc aaaaaacagt
    12841 ttttgtttcc ggaagcggga atgtagccat ttatgcaatc gaaaaagtgc aagagctagg
    12901 cgggaaagtt gttacatgtt cagattctag cgggtacatc tatgatccag aaggaataga
    12961 tgtgaaacta ttgaaagaag tcaaagaaat caatagacaa cgtttgacgg aatacgcaaa
    13021 ccaacgtcct tctgctattt atcatgctgg agaatctgtt tggacattga aagaaaaagc
    13081 ggatatcgct ttgccatgtg ctactcaaaa tgaaatagac gaaagcttgg ccgaaatctt
    13141 agtagaaaat gggatcaaaa tcgtagcaga aggcgctaat atgccatgta cagtaaaagc
    13201 tgtttctgtt cttcatgaag caggcgtgtg gtattgtcca ggaaaagctg ctaatgctgg
    13261 aggagtagca gtatctgctt tagaaatgag tcagaacgtc cagcgattga cttgggaaag
    13321 agagcaagtg gatgagcagc tggatcaaat catggaaacg atttaccata cgtgtcgcca
    13381 aacagcagcc gagtacggcg acgaaaaaaa tctgcttctt ggagcaaatg ttgctggatt
    13441 tgaaaaagtt gccaaagcaa tggcagcaga aggtttagtt tgaaaaatga aactttcttg
    13501 gaaatattgt cagcattttg tttatttgct tgaaaaataa taaaaaaaaa cgtaaagtat
    13561 tacctgtaga gtaaacctat aaggaggaga acaaatggca cacattcatt ttgactattc
    13621 aaaagtagca ccgtttgtca acgaacaaga gttagggtac atgcaaagcc aagtaacagc
    13681 agcccacaac gaattacggg aaggtactgg cgcaggaagc gacttccgcg gctggatcga
    13741 tttaccaacg aactatgaca aagaagaatt tgcacgtatc aaagaagcag ctaaaaaaat
    13801 ccaatctgat tctgaagtat tagttgttat cggtatcggt ggttcttatt taggcgctcg
    13861 tgcagcgatc gacttcttga atcacacatt cttcaattta ttagatagca acacaagaaa
    13921 agcaccacaa atcttctttg ccggaaattc aatcagctca acatatattg ctgacttgat
    13981 cgaagtaatc ggtgatcgcg acttttcagt aaacgttatt tcaaaatctg gaacaactac
    14041 tgaaccagcg attgcgttcc gtgtattcaa agagcttctt gttaagaaat atggacaaga
    14101 agaagcaaac aaacgtatct acgctacaac agataaagca aaaggtgcag ttaaagtaga
    14161 agcagatgca gaaggttggg aaactttcgt gattcctgat gacatcggcg gacgtttctc
    14221 tgtattaact gctgttggtt tgttaccaat cgctgttagt ggtgcagata tcgatgcatt
    14281 gatgcaaggt gccgctgatg caagcaaagc atactcaagc gacaaactag aagaaaacga
    14341 agcttaccaa tacgcagcaa tgcgcaacat tctttaccgt aaaggcaaag taactgagtt
    14401 gttgatcaac tatgaaccag gaatgcaata cttctcagaa tggtggaaac aattgtatgg
    14461 tgaatctgaa ggaaaagacc aaaaaggcat ctatccttca agcgcaaact tctcaactga
    14521 tttgcactct ttaggacaat tcatccaaga aggacgtcgc aatatttttg aaacagttgt
    14581 gaaggttgaa aaacctcgta aatcaatcac aattcctgaa caagcagaag atttagacgg
    14641 tctaggctac ttgcaaggaa aagaagtgga cttcgtaaat acaaaagcat ttgaaggaac
    14701 attgcttgct catacagacg gtgacgtgcc taacttgcta gtcaaaattc cagaaatgga
    14761 tgcttataca ttgggttata caatgtactt ctttgaaatt gctgtaggta tttcaggtta
    14821 cttgaatgga atcaacccat tcgaccaacc aggtgtagaa gcttacaaga aaaacatgtt
    14881 cgcacttcta ggcaaaccag gctttgaaga tcttgctaaa gaattgaacg aacgcctgta
    14941 agataagggt ttttcgctag atttaaataa tcagtgatag aaacatgttc gctttaaaat
    15001 gaaaactggt acccatttag gtactcaaac atactgcatc gtttattcag aataaggggc
    15061 tgtgacaaga cgtttgtcac agtcccttgt ttgtatagaa atcgattgat tgccttgctc
    15121 tttagtttgt tttaggactt taagaacagt gaggctgact tcgaaggtta attctgtttt
    15181 tttgttttct agaatcagta agcaaaaaag cactcacttc gagtgctttt ttgcgatata
    15241 ttcttagctt aagataggcc taatttttct tttacagatg agggtacctt atcgctggta
    15301 attacttttt tctctatgat ccgcttttcg cttgctttca actgcaagat cgttccttcg
    15361 gggtaagcag aaccattggc agagtcacca gaagtaatga catcgaaatc taactgtctt
    15421 ttttcaccgt tttcagcata agcatctacg ttgtatttaa aacctcttcc aattacttcc
    15481 ccattatctg ctttgatatc agtttctgca ggcattggag ctttgactac cgcataataa
    15541 tctttaccta cataagtaga ctgatagtag tcccaacctt tccatcctat aaaagcaatc
    15601 aatatgataa cgatcaaccc taaaattttt ttcatgaaag atctcctttt ttatttttat
    15661 tatacccgtg gtgctagtta attttaatat cctaaagaat atctcaaagt tccttcaaaa
    15721 cgtcgttgtg ctcgacttaa tagaacactg taagccagta atatgctttc aaatctactc
    15781 tctagccctt taacagaacg tgagttgaaa ttttgacaac ctaatttttc taaggaagaa
    15841 aaaacagtct caactgt
//
LOCUS       DAZUMB010000060        15849 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000060 DAZUMB010000000
VERSION     DAZUMB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.061"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..751
                     /locus_tag="ACG80L_001649"
     CDS             68..751
                     /locus_tag="ACG80L_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS5023879.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="ACG80L_001650"
     CDS             753..1589
                     /locus_tag="ACG80L_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5023880.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="ACG80L_001651"
     CDS             1600..3354
                     /locus_tag="ACG80L_001651"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023881.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="ACG80L_001652"
     CDS             complement(3630..3971)
                     /locus_tag="ACG80L_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5023882.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="ACG80L_001653"
     CDS             complement(4039..6210)
                     /locus_tag="ACG80L_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5023883.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="ACG80L_001654"
     CDS             6421..7278
                     /locus_tag="ACG80L_001654"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5023884.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="ACG80L_001655"
     CDS             complement(7349..8206)
                     /locus_tag="ACG80L_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5023885.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="ACG80L_001656"
     CDS             complement(8191..10893)
                     /locus_tag="ACG80L_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5023886.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="ACG80L_001657"
     CDS             complement(10906..12195)
                     /locus_tag="ACG80L_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS5023887.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="ACG80L_001658"
     CDS             12345..13391
                     /locus_tag="ACG80L_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5023888.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="ACG80L_001659"
     CDS             13393..15546
                     /locus_tag="ACG80L_001659"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS5023889.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4504 a         3304 c         2926 g         5115 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatg
//
LOCUS       DAZUMB010000061        15786 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000061 DAZUMB010000000
VERSION     DAZUMB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.062"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..766
                     /locus_tag="ACG80L_001660"
     CDS             14..766
                     /locus_tag="ACG80L_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5023890.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            763..1677
                     /gene="pfkB"
                     /locus_tag="ACG80L_001661"
     CDS             763..1677
                     /gene="pfkB"
                     /locus_tag="ACG80L_001661"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5023891.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1695..3626
                     /locus_tag="ACG80L_001662"
     CDS             1695..3626
                     /locus_tag="ACG80L_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5023892.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3737..5296)
                     /locus_tag="ACG80L_001663"
     CDS             complement(3737..5296)
                     /locus_tag="ACG80L_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS5023893.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5451..7685
                     /gene="pcrA"
                     /locus_tag="ACG80L_001664"
     CDS             5451..7685
                     /gene="pcrA"
                     /locus_tag="ACG80L_001664"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS5023894.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7702..9738
                     /gene="ligA"
                     /locus_tag="ACG80L_001665"
     CDS             7702..9738
                     /gene="ligA"
                     /locus_tag="ACG80L_001665"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS5023895.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9844..10149
                     /gene="gatC"
                     /locus_tag="ACG80L_001666"
     CDS             9844..10149
                     /gene="gatC"
                     /locus_tag="ACG80L_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS5023896.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10149..11621
                     /gene="gatA"
                     /locus_tag="ACG80L_001667"
     CDS             10149..11621
                     /gene="gatA"
                     /locus_tag="ACG80L_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS5023897.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11618..13048
                     /gene="gatB"
                     /locus_tag="ACG80L_001668"
     CDS             11618..13048
                     /gene="gatB"
                     /locus_tag="ACG80L_001668"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS5023898.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13058..14113
                     /locus_tag="ACG80L_001669"
     CDS             13058..14113
                     /locus_tag="ACG80L_001669"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5023899.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14224..15597
                     /gene="rlmD"
                     /locus_tag="ACG80L_001670"
     CDS             14224..15597
                     /gene="rlmD"
                     /locus_tag="ACG80L_001670"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5023900.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15600..15728
                     /locus_tag="ACG80L_001671"
     CDS             15600..15728
                     /locus_tag="ACG80L_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5023901.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5368 a         2899 c         3471 g         4048 t
ORIGIN      
        1 ggtgatcgtg aaaatgctta cagaggaacg cagacaaaaa atcctagact tgcttgaaca
       61 gcagaaaatc gtgaaatcac aagatcttgt aaaccttttg aatgcttctg aatcaacgat
      121 aagacgtgat ttacaagagc tggagaatag tgggtttcta gaaagagttc atggcggagc
      181 taagaaagag caattattga attttgagca gaatatgagt gaaaaatcac tcaaaaacgt
      241 tcacgaaaaa cagcaaatcg ccaaatcggc agctgaaacc attaatgacg aagatgtcat
      301 ttatttagac gctggctcta ctacgttaga aatgattcct tttctgcaag gaaaacagat
      361 cacagtagtg acaaattccg ttcaacatgc ggcaaattta gttgatttgg agcttgctac
      421 cattatttta ggtggaacga taaagctggc aactaatgct gttttaggat ctaatgcgat
      481 agaacaacta aaaaattttc attttaataa agcatttatg ggaatgaacg gtgcccatct
      541 tgaacaagga ttcacgactc cggatccaga agaggcagct gttaaacgtt tagccatgaa
      601 aaattccgaa gagacatttg tattgattga tcataccaaa ttcaaccagt tgtccttcac
      661 ctctgttgca cctttggaag cagccacgat cataaccgag cgttgtccat tagaattcgt
      721 taatgaattc cgtgaaaaga caacaattaa ggaggcaata caatgattta cacagtaaca
      781 cttaatccat caattgatta tatcgtacgc gtagatgggt taaaactagg cgatttgaac
      841 cgaatgaagg aagacttcaa gttacctgga ggaaaaggga tcaatgtttc ccgtattttg
      901 aaacggatcg attctcaatc tacagcacta gggtttttag gagggttcac tggcgaattt
      961 atttcgaagt ggcttgaaaa agaacaaatc cagtctttgt tcacaaccgt aaaagaagat
     1021 acaagaatca atatcaaact aaaatcagac actgaaactg agatcaatgg cttaggccca
     1081 tctatttcag atgaagaaat cgatgagttg aagcaagcat taggaacagt aggacaagga
     1141 gatatcgtcg tattgtcagg aagcgcgcca gctagcctgc gaaaaggatt ttatcaagaa
     1201 ctgattgata tcattcgaga aaaaggtgca gatttcgtga ttgatacaac aggtgatgac
     1261 ctaaaaaatg cacttgttaa gaaaccgttg ttgatcaaac caaacaatca cgaattagct
     1321 gagctttaca acacaacctt ccaatctgta gaagaaatca ttccgtatgg gaaaaaatta
     1381 ctagctgaag gggcacagaa tgtgctgatc tcaatggctg gagacggtgc tttgctcttc
     1441 acggaacaag gaacttataa aacgaatgta ttagttcgcc cattgaaaaa ttcagtaggt
     1501 gcaggggatt caatgattgc tggctttatt ggggcttaca gccgtacgaa agatccaatc
     1561 gaagccttta aatggggagt tgcttgcgga agcgcaacag ctttttctga tgacttggca
     1621 gctcgcgaat tgattgatga gttgctgcca gaagttgaaa tcacaaaaat tgattagttt
     1681 caaggaggat aatcatgaat atcaaggatt taatggtcaa agaagcgatg atcatggatt
     1741 tgcaagcgac tgataaaaaa ggcgcaatcg atgaaatggt ccaaaaaatg tttgatgccg
     1801 gccggatttc cgatatcgaa acatataaag aaggaattct agcccgcgaa gcacaaactt
     1861 ctacaggtct tggtgatgga atcgccatgc ctcacgcgaa aaacagtgca gttaaagaag
     1921 caacagtttt attcgcaaaa agccaaaaag gagtagatta tgaagcctta gacggacagc
     1981 caacttatct gtttttcatg atcgcagctc cagaaggcgc aaacgacaca catttgcaag
     2041 cattagcagc actatctcga ttgctgattg atgcagattt cgtaggaaaa ttaaaagaag
     2101 caaatacacc agaagaagtc caagacttgt tccaaacagc tgaagagcaa aaagaagcag
     2161 aagaaaaagc agaacaagaa acacaagctg cagaagcttc tgctcatcca gaaagaaaat
     2221 tcgttgttgc cgttacagct tgtccaacag ggatcgctca cacttacatg gcagaagatg
     2281 ctttgaagaa aaaagcgaaa gaaatgggtg tggagatcaa agtcgaaacg aacggttcag
     2341 aaggaatcaa aaaccgattg actgctgaag acatcgcccg tgcagaaggt gtaatcgttg
     2401 cagcagacaa aaaagtagag atgaatcgct ttgacggaaa agaactgatc aatcgtcctg
     2461 tcagtgatgg tatccgtaaa ccagaagagt tgatcaatct agcccttagt ggtactgcac
     2521 cagtattcca tggtgatgga aaagaaagca gttcagaaga aggaaatgcg gatggtacta
     2581 tcggacaaag aatttacaaa gatttgatga acggcgtatc acacatgtta cctttcgtta
     2641 ttggtggagg aatcgctatt gcgctttctt tcatgatcga ccaatttatt ggcgtgccac
     2701 atgatcaatt atcaaatcta gggaattaca atcaagctgc tagctggttc aaccaaatcg
     2761 gacaagctgc atttggtttc atgctgccgg tattagcagg atttattgct tcaagtatcg
     2821 gtgaccgccc tggtttgatc gttggatttg ctgctggtgc attagctaat acaggcggtg
     2881 caggattctt aggtgcactg attggtggat tcttagctgg ttatgtcatc gtatttttac
     2941 gtaaattatt caagaactta ccaaaatcat tggatggaat taaaacgatc ctcttttatc
     3001 cagtgtttgg cttactgatt actggtttct tgatgttgct ggtcaatgtc cctatgaaag
     3061 caatcaatga tgcattgaac ggtttcttga caagtatgag tggttcgaat gctgctttac
     3121 ttggtgcatt attagctgga atgatggcgg cagatttagg cggtccaatc aacaaagccg
     3181 cttatgtgtt tggtacagca actttagcta cgactgttgc aactggcgga agtgtcgtga
     3241 tggcttctgt tatggctgga ggaatggtac ctccattggc aattttcgtt gctacacgtt
     3301 tgtttaagaa taaattcagc aaaacagatc aagatgctgg tttgacgaac atcgtgatgg
     3361 gactatcgtt tgtcacggaa ggatcgattc catttgcagc agctgacccg attcgtgcca
     3421 ttccaagttt tattattggt tctgctttaa ctggcggatt agtcggagct ttcggaatca
     3481 aattactggc tcctcacggc gggattttcg ttgtattctt actgagtcac ccggtcatgt
     3541 atttggtgtt tattgctatc ggagcagtcg tatctggtgt gatttatggt ttattgagaa
     3601 aagctcctgc agtagcagaa gcatagaaat aaaaaagaag aggctgggtt ttcccagcct
     3661 cttctttttt tatagaatca tatagaatca agtagtgtag atgaatagtt gttgtaaaat
     3721 agtttttcca tgttcgttaa aatgattgct tcaaatttgt gatagaagat ttagcctcga
     3781 tttcctgctt gattcttggc aagtttgctt catcagtcaa gataaccaaa atatctccta
     3841 cattcatgat cgtatcaccg tgagtcaaga tttcctgatt tcctctgcgt atagagatta
     3901 gcagcatatt tttaggccaa ttgaagtctc gtaccattaa tccgtctaaa gaactctctg
     3961 ccgttactgg aaaatcaaaa gttgtccttg caccttttag tgaggcggtc tgagtatttc
     4021 caagtagacg ttccaataat gattcgtaaa ttggattacc gccgagaagg tcattgacga
     4081 tataagcaac aagagcagcc aatcctaaag gcatcagatg attaagagag ccaaccattt
     4141 ctgtcactaa aataattgct gtcagcggtg cttttccaat cgctgtgaaa taaccagcca
     4201 tggcaataac gacaaaatcc ttgatgtaga ttggatccat cccgaaatca accataatgt
     4261 ttccataaaa gctaccgatt aaagccccca atgtcaatat cggaaggaaa atcccccctg
     4321 gaagaccccc accgtaagaa atcatggaaa aaacgaatcg aagaacaaac aaaccgataa
     4381 tcataatgat tggcaaggaa gcatgtccaa aagctaaaac gatttgattg cctccgccta
     4441 gagtactagg ccataaataa ccaattggca aaatcagcaa gaaaggaacg aacccgtgaa
     4501 aataagctgg taaaggcaat cggttgtaaa attttggcaa agccagcaag acattctgat
     4561 aaataaaacc taagatgcct agaagtactc ctaagatcac taaggaacca taatatttca
     4621 acggcaaaga aggaaggtca cctagatata aaacaggtct caagccgaaa aagtaaagag
     4681 agacgaagtt agcagtgatt gctgaagcaa atgatgtcag ccaaacaagt ggagaaaagg
     4741 agtgatggat ttcttctaaa acaaagagta atcctgcgat aggagcattg aatgctgctg
     4801 caagtccgct acttgcaccg ctagaaatta aaatcttttc ttctgaagat ggactccgaa
     4861 acaattgtcc agctccttgt ccaatggatg cgccaagctg tatagaaggt ccttctctac
     4921 ctagaaaaag gcccgcacca actgaaagta caccgccaat gaactttttc cataaaacag
     4981 agaaccagtt cattgataat tccccgcgaa gctgtccctc tacttgtgga atcccgcttc
     5041 ccttgatatc cggttcattt ttgaccagat aacccacgaa aaatgctgca aggatgcagc
     5101 aaatagctag gccaattaag taaatcggct gttcccgact catttcatag atacttcgaa
     5161 ctaaatcgct tagcttttcg atagataatc gaaacaggct aacgacgatt cctgtcagca
     5221 gtccaacaaa tattcctttt agaataaaat atatctgagt cttatctaat ttcttgattt
     5281 ttcctttttt ttccattcaa acgcctcgct ttcttttaat ttagcgcgaa atcaacgaat
     5341 tgacaaggaa aaatccataa aaagaaacaa atgattttga actagctagt ttaatgaaag
     5401 ccaagaaaat gctataatta acaagattga taaaagaaag gatgttgtta atgtcatcca
     5461 aagctgaatt attaaacggg atgaaccccc gtcagaaaga agctgttctt cacactgacg
     5521 gaccattact gctaatggca ggtgcaggaa gtggaaagac gagagtgctg acacaccgca
     5581 ttgcctattt gattgaggaa aaagaagtca atccttggaa catcctagcg attactttta
     5641 ccaataaggc tgcaaaagaa atgaaagaac gtgtcaatgc gattctcgca tctggaggcg
     5701 aagatgtctg ggtatcgact tttcactcga tgtgtgtgcg tattttaaga agagatgttg
     5761 attttatcgg gtataaccgg aactttacaa tcatcgattc ttctgaacag ctgaccttga
     5821 tgaaacggat actaaaagaa ctgaacattg atccgaaaaa atatgatcct cgaagtatat
     5881 taggaacaat ctctcaagca aaaaacagcc tgcagacccc tcaggatttt gcaaaaatgc
     5941 aaggcagtta ttacgaagag attgcggcaa aatgttatgc tgcttaccaa aaagagcttc
     6001 aatataatca atgtatggac tttgacgacc tgatcatgaa tacgattcga ttatttgaag
     6061 aacatcctga ttcactcacg tattatcaaa acaaattcca ttatattcat gtagatgagt
     6121 accaagatac aaatcacgca cagtatacgc tagtcaattt attggcaggt agatttagga
     6181 acctgtgcgt cgtgggagat gcagatcaaa gtatttacgg ctggcgcggt gcagatatgc
     6241 agaacatctt ggattttgaa aaagattatc cagatgctgc agtaattttg ctagaacaaa
     6301 actatcgctc cacgaaaaac atccttagcg cagcaaacca agtcattgaa aacaatagca
     6361 atcgaaaacc aaaaaacttg tggacagaaa acaaagaagg aaacaagatc acttactacc
     6421 gtgcagataa tgaacgagac gaaacccgtt ttatcgtaga ccggatgcaa gaagagattc
     6481 gaagcaatca tcggaattac ggcgactttg ccatcttgta ccggacgaac gcacaatctc
     6541 gtgtaatgga agaaactctg ttgaaagcaa atattcctta taaaatggtt ggagggcaca
     6601 agttctacga tcgtaaagaa atcaaagata ttttagcgta tttgaatgtg ctggtaaatc
     6661 ctcaagattc aatcagtttt gagcgtatcg ttaattcgcc caaacgaggc atcggaccag
     6721 gatctatcga aaaactacgc agttttgctt ctcttcatga atggccactg cttgaagcag
     6781 cgcaaaatgt ggatctggca aatatcggtg gaaaagcagg acagcaatta ggtgcatttg
     6841 gagagatgat ccaagaagtc actcagatga tcccatactt gacagtgaca gaattgacga
     6901 aagaggtttt ggatcgtagc ggatatctag aagacttaaa aatccaaaat acattagaag
     6961 cacaagcacg catcgaaaac ttggaggaat ttttaacggt cacacaagaa ttcgacaaac
     7021 aattcgaaca gcaaaatgaa gaagacgctg atgcaccaga agaaaaacta acggtcttct
     7081 taaatgatct ggcacttgtt tccgacattg acaatttaga agaagatgct tcccaagtga
     7141 cattgatgac tttgcatgca gccaaaggat tagaatttcc agttgtattc ctgattggtc
     7201 tagaagaggg ggtttttcct ttatctcgag cattgatgga agaaagcgaa ctggaggaag
     7261 aacgccgtct cgcatatgtg gggatcacgc gagcagaaga agcgttgtat ttaacaaatg
     7321 ctttctccag aacattatat ggacggacgc aatataaccg accttctcgt tttgtagaag
     7381 aaatcgatca ggagttactg gaaatcgaag gcatgcgtcc tacgcctaaa aaaacgcctg
     7441 tattcgctaa aaaaacagca tatagctata aacagccaga gacagcggtt gttccctcta
     7501 aaagtgctac tggaggggaa aataacagct ggaaacctgg agataaagtc aaacacaaaa
     7561 aatggggtct aggaacagtc gtccgagtaa gcgggacgtc gaaggatcta gaactagatg
     7621 tggctttccc aagtcaagga gtaaaacgtc ttttggctgc ctttgcgcca attgaaaaag
     7681 cttagtagtg gagggtaaat gatggaacat gaaatgacga ttgacgaagc tgctaaacga
     7741 gcagaagaac ttcgtacaag actgaaccaa tggtctcgcg aatactacgt agaagataaa
     7801 ccaacagtgg aagactatgt ttatgataaa gaatacgcag aattagtagc aatcgaggaa
     7861 caatatcctg atttgatcac gtctgattct cccactcaac gagtaggcgg aaaagtatta
     7921 gaaggttttg aaaaagtcac tcatgatatt ccgctttaca gtttgaacga cgttttcagc
     7981 aaagaagagc tgatagcttt tgatcaacgt gtacaaaaag cagtgggacg tgtagttgac
     8041 tattgctgtg aactaaaaat agatggactt tccgtttctt tgcgttatga agatggaaat
     8101 ttcgtgagag gtgcgactcg cggggatgga actgtaggag aaaacatcac tgaaaaccta
     8161 aaaactgtcc ggtcggtccc aatcaaatta aaagaaccaa tgaacatcga agtaagaggc
     8221 gaatgtttca tgccaaaacg gtcatttgtc caattgaacc aagacagaga agcagaggga
     8281 aaagatatct ttgcgaatcc tcggaacgcc gcagctggtt ctttgcgtca gttagattct
     8341 aaaatcacag cgaaaagaaa tctggacact tttttataca cagtagctga tttcggtcct
     8401 atgcaagcaa aaacccagta tgatgcgcta gaagagctag aaaaaattgg attccacacg
     8461 aatcgggaaa aacgtctatg tcattcgatt gatgaagtgt ggtcatacat tgaagaatat
     8521 catgacaaac gtgtggattt gccctatgaa atcgatggaa tcgtcatcaa agtgaatgaa
     8581 ttctcgctac aagatcaact tggttttact atcaaagcac cgagatgggc agcagcttac
     8641 aagtttccgc cagaagaagt tgaaacgcta atcgaaaaca tcgaatggac agtcggtcga
     8701 acaggggtag taacgccaac agctatcatg acaccagtcc gagtagcagg aacgacagta
     8761 agccgtgcca gcctgcataa cggtgattat attaagttga aggatatccg cttgaaagat
     8821 acagttctga tttataaagc gggagacatc atcccagaag tttcgcaagt cgtgttagat
     8881 aaacgaccta aagacagcga agaataccaa ttgcctacac attgtccagt ttgtggaagt
     8941 gaacttgtcc atttggatga agaagtagcc ttacggtgca tcaatcctaa atgccctgct
     9001 caaatgaaag aaggtttgaa ccactttgtt tcaagaaatg cgatgaatat cgatgggttg
     9061 gggccgcgcg tactagaaca gatgtatgat aaaaaactag tagcagatgt tgctgatctt
     9121 tacaaattga cagaagaaga gttattgacg ttggacaaaa taaaagaaaa atcagcaaat
     9181 aatattttga cagcaatcga caacagcaaa gataattcgg tcgagcggtt gattttcggg
     9241 ttaggaatcc gacatgtcgg agcgaaagca gcaaaaattt tagcagaaca tttcggtgat
     9301 ctagaaacat tgagtaaaag tgactatgag tcgattattg cgcttgatac gatcggtgat
     9361 atcattgccg acagtgtagt cacttatttc agtaacgaag aagtccatga attgatgaat
     9421 gaattaaaac aagcaggcgt caatttcgaa tacaaagggc ttcgaaatgc ccaattgcaa
     9481 gaagtcgagt ctccttttaa agaaaaaaca gtagtcctta ctggtaaact aacacgattt
     9541 actcgtgaag aagcaaaaga gacgatcgaa aatcttggcg ggaaagtgac tggtagcgta
     9601 tcgaaaaaaa cggatatcgt ggtagcagga gaagatgctg gcagcaagct gaccaaagca
     9661 caagaattag gaatcgaagt ttggacagaa gatcaaatgg cagatgcatt ggctaaaagt
     9721 cgctcagtag aagaatgaaa aatgagtttt ttcagacaaa tttcaataaa caatttcaga
     9781 cacggcactc ttgtgttaaa atagagagca aagtttttaa ctcgtttaga aagaaggggt
     9841 agtatggcga ttagtgaaga acaagtgaaa cacgttgcca aattagcaaa acttgcgttc
     9901 gcagatgaag aacttgcaag ttttaccgac caattaggta aaatcatcga tatggttgaa
     9961 atgctggaag aagtagatac ggaaggtgtt ccatttacgt cgaatgtggt tgaaacagta
    10021 aacgtgatgc gacaagatcg ggcgcaagct ggatggaacc gcgatgaatt attgaaaaat
    10081 gtgccggaaa cagaagacgg ctttatcaaa gtacccgcaa tcatcgataa tggggaggct
    10141 ggcgcataat gacagaatta cacaatcgtt ctctagaaga actgcatcat atgcttgtat
    10201 cgaaagaaat cactgtacaa gatttgacaa aaacaacatt tgaacgaatc aaagaaacag
    10261 agcctgaggt cgaggcattc attacattga acgaagaaaa agcattggaa caggcaaaag
    10321 ctttagatga aaaagggatt gatgaaagca acgtccttgc tggtattcca attggtatca
    10381 aggataatat cgtaacaaaa gatatcttga caaccgctgc atctaagatc ctttacaatt
    10441 ttgaaccaat ttatgatgca acggtcatgg ataaaatcta tcaagcagat atgatccctg
    10501 ttggtaagct aaacatggat gaatttgcta tgggtggaag tacagaaact tcttatttca
    10561 agaaaacaaa aaatgcttgg gacccaacaa aagtacctgg tggctcttct ggtggttctg
    10621 cagcagcagt tgcagctgga caaattccag tttctttagg tagtgatact gggggaagta
    10681 tccgccaacc agcagctttt aacggcatcg tagggatgaa acctacttat ggccgtgtct
    10741 cacgttttgg tttgattgcc tttgcttcat ctcttgacca aatcggacca atgacccgta
    10801 cggtgaaaga taatgcatta gcattgacag ctatcagcgg ttatgacgaa aaagacggta
    10861 cttcttcagg cgtttcagtt cctgattttg cagagggact gacaggcgac atcaaaggaa
    10921 tgaagattgc tttacctaaa gaatacttag gcgaaggtgt ggcccctggc gtaaaagaag
    10981 ctgtattgaa agcagcagaa acattccgtt cattaggtgc tgttgtggaa gaagtcagct
    11041 tgcctcattc aaaatacggt gtggctgttt attatatcat tgcctcatca gaagcaagtt
    11101 ccaatttgca acgattcgat ggtatccgct acggttatcg ctctgaaaat gtgaaatcat
    11161 tagatgatgt atatgtcaac tcacgttcag aaggattcgg tgaagaagta aaacgccgta
    11221 tcatgttagg tacattctcc ttaagcgcgg gttattatga cgcttacttc aaaaaagcag
    11281 gacaagtccg tacattgatc aaacgagatt ttgaaaaagt ctttgcagat tatgacttga
    11341 tcatcgggcc atcttcacca acagttgcat tcggaatcgg tgagaacatt aacgatccaa
    11401 tcacaatgta catgagtgac atcttaacga tccctgtcaa cttggctgga cttccaggta
    11461 tgtctgtacc ggcaggttta tcagaaggat taccagttgg attgcagatc atcggtaatt
    11521 attttgatga aaaaacgatg tatcaagcgg catatgcgtt tgaacaagca acagaattcc
    11581 ataaacaaca accagctatt ttaggaggga aagacgaatg aattttgaaa cagtcattgg
    11641 actagaagtc cacgtcgagt taaaaaccaa ctcaaaaatt ttctctccag cgccagctca
    11701 ttttggtgca gaaccaaaca gcaatacaaa cgtgatcgac tggggttatc caggcgtgct
    11761 gccagtaatg aataaagctg cgctggaatt cgggatgaaa gcagctttgg cattaaactg
    11821 tgaaatctct aaagatactc attttgaccg gaaaaattac ttttacccag ataatccaaa
    11881 agcatatcaa atttcccaat ttgatcaacc aatcggtcat gacggatgga tcgaaatcga
    11941 agtagaaggt aaaaagaaaa aaatccggat cgaacgtgtc catttagaag aagatgctgg
    12001 aaaaaacatg catggtattg gcggttattc ttacgttgat ttgaaccgac aaggcacgcc
    12061 tttgatcgaa atcgtttctg aagctgatat gcgttcacca gaagaagcat acgcttatct
    12121 agaagcattg cgatcaatta tccaatttac agaagtcagc gatgtgaaaa tggaagaagg
    12181 ctctatgcgc tgtgatgcca atatttctct tcgcccttat ggacaagaag agttcggtac
    12241 gaaagcagaa ttgaaaaact tgaactcttt atctttcgtg aaaaaaggtt tagcttttga
    12301 agaaaaacgt caagcaaaag ttcttctttc aggaggagaa atccaacaag aaacacgccg
    12361 ttttgacgaa acaacgaata aaacagtttt gatgcgtgtc aaagaagggt ctagtgatta
    12421 tcgttacttc ccagagcctg atatcccgaa attcgtgatc gatgacgaat ggattcaaaa
    12481 agtaaaagct agtttgcctg agatgccagc ttctcgtcgc gaacgctata tccgtgagtt
    12541 tggacttcca gagtatgacg caatggtatt gacacttaca aaagaaatgt ctgatttttt
    12601 tgaagctgtt ttggaaaatg gtgcagatgc caagcaagca tctaactggt tgatgggtga
    12661 agtttctgct tacttgaaca gtgagaaact ggaattagct gagacaaaat tgacacctga
    12721 gaacttagca ggaatgattc gtttgatcgc cgatggaacg atcagctcaa aaatcgccaa
    12781 aaaagtcttc cgtgaattga tcacaaatgg cggagaagcc caagaagtag ttgaaaaaaa
    12841 tggttgggtg caattatctg atccgaacaa actactgcca atcattaatg aaattttgga
    12901 taacaaccaa caatctgtcg atgatttcaa aaatggaaaa gatcgtgcgg ttggcttcct
    12961 agtcggtcaa atcatgaaag caactaaagg acaagcaaat cctggtgtcg tgaataagct
    13021 acttaaagat gaattagcga aacgatagag gctgaaaatg aaaaaagcac gcgtaattta
    13081 taaccccacc tcgggaaaag aattattgaa aaaaaatttg gcggatatcc tacaggcatt
    13141 agaagaagca ggatttgaag ccagcgccta tgcaactaca ccagaaccag attcagccaa
    13201 gaatgaagca tgtagagtcg cagaactagg ttttgatcta gtagtggcag ctggtggcga
    13261 tggcacaatc aacgaagtgg ttaatggaat cgctccgctt gaacaccgtc ctaaaatggc
    13321 catcatccca gctggtacaa cgaacgacta tgctcgagca ttaaaaattc ctagagataa
    13381 tattaaagca gcagcagaag tgataaaaaa acaccaaacc gtaaaaatgg acatcggcct
    13441 ttctgataac agttacttca tcaatatcgc tgcaggtggc tatttgacag aattgactta
    13501 tgaagttcca tcagaactaa agagtatttt tggttatctt gcttatcttg cgaaaggagc
    13561 agagatgctc cctcgagtaa agccaatcaa aatgcgcttg aagtatgatg aaggggaata
    13621 tatcggaaat gcttcgatgt ttttcctagg gctgacgaac tctgttggag gattcgaaca
    13681 gatcgcgcca gatgccaaat tggatgatgg gaagttttct ttaatcattg tgaagacagc
    13741 aaatatcttt gagatcttgc atattgcagc actcatgctg aatggcggtc gacatgtcga
    13801 tgatccaaga ataatctata ccaaaacaag cagtttgttc gttgaaacag aagatgatat
    13861 caatcgacca gtaatgatca acttagatgg tgaatacggc ggggaagcac cgatgcattt
    13921 ccaaaatctt catcaacaca ttgagttctt tgccaatacc gatcagattc ctgatgaagc
    13981 aatcacaggt acagatgaag aagaattaga ggttgtcagc aaagaattcg taaaagaagt
    14041 cgaacgttta acggatgaag atatcgacgg tgacggaaaa atagcagacg aaacacctaa
    14101 tcaaaagaat taatcaaaag ccggtgctga acgtagtaag gtcagtgccg gttttagttg
    14161 tgtaattagc tgatttatag tagagttcag taggattgaa aaataggctt aaaggagcaa
    14221 aatatgacag aacagcaaat agtaaagaaa aacgaacgat atacagtcga aatcgtcgac
    14281 ttgagttatg aaggaatggg ggtagcaaaa atcgatggtt atccattatt tatcgaaaat
    14341 gcactccctg gagaagaagt agaaatacta gtagtaaaag caggcaataa gtttggatat
    14401 ggaaaagtcg aaacatttat tacacaatca cctgatcgtc agcctgtaga aaacaatgca
    14461 ttacttcgca taggtatcgc acctttagcc catttgaagt atgaacaaca gcttgtcttc
    14521 aaacaaaaac aagtagagca tgtaatggcg aagattgcta agatgccaga agttcctgta
    14581 cttccaacgg tcggaatggc agaaccattc ggctaccgta ataaagcaca gatccctgtt
    14641 cgccgtatcg atggacagtt agccaccggt ttttacaaaa agaatagcca tgatctagtc
    14701 cctatcgaag atttttatat ccaagatcct gtcatcgatc aagcaatcaa agtagtcaga
    14761 gatatcttgc aacggttcca agtacgagga tataatgaag aaaaaaatga aggacagctt
    14821 cgtcacatca ttatccgtcg tggtcattat tcccatgaaa tgatggtcgt tttagttact
    14881 cgtaaagaaa aattctttaa atccaaggaa attgctgaag ctattcatga agaattgcca
    14941 gaagtggttt cggtcatcca aaatatcaat gaagaaaaaa caaatgtgat tttaggagac
    15001 aaagaaaaag ttttatatgg cagagactat atcgaagatc agctattagg aaaaacctat
    15061 cgaatctcag caaagtcatt ttatcaagta aatactcaac aagcagagta tctgtaccaa
    15121 acagccatcg agtttgccga gttagataaa aaagacattg tgatcgacgc ttattcagga
    15181 ataggtacga ttggcttgtc tttagcagac agagtggata aggtgtatgg gatggaagtt
    15241 attccagaag caattgaaga tgcacagttc aatgctttaa ataataaaat cgaaaatgtt
    15301 cattatgaag tcggaaaagc agaaaaagtt atgaaaaaat ggcaagaaaa agggatcaaa
    15361 ccatctgtga tcgtagttga ccctccacga aaaggattag atcatcattt tattgaatca
    15421 gcaatcgaaa tgtcaccaga acgaatcgtc tatatctcat gtaatccggc aacttttgca
    15481 agagatgcta agttatttgc agaagcaggt tatgccgtga agaaagttca gcctgtagat
    15541 cttttcccgc aaacccatca tatcgaatta gtcgcttcat tcgttaaagg agaataaaaa
    15601 tggaccaaaa agaaaaaatg aaagaattga tcaaaaagaa acaaggtgga ggccgatcca
    15661 aacaaatgga aacacctaag aatgatcgta aaaacatgag aaaaggccct aaaatcttta
    15721 ataaatagac gtgctaaaga aagactaaga agtgttaact tttgttattg tttcttagtc
    15781 ttttta
//
LOCUS       DAZUMB010000062        15717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000062 DAZUMB010000000
VERSION     DAZUMB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.063"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACG80L_001672"
     CDS             138..1475
                     /locus_tag="ACG80L_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS5023902.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACG80L_001673"
     CDS             1523..3652
                     /locus_tag="ACG80L_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS5023903.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACG80L_001674"
     CDS             3627..4316
                     /locus_tag="ACG80L_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023904.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACG80L_001675"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACG80L_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACG80L_001676"
     CDS             complement(5546..5740)
                     /locus_tag="ACG80L_001676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023905.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACG80L_001677"
     CDS             complement(5790..6230)
                     /locus_tag="ACG80L_001677"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS5023906.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACG80L_001678"
     CDS             complement(6234..7079)
                     /locus_tag="ACG80L_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS5023907.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACG80L_001679"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACG80L_001679"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS5023908.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACG80L_001680"
     CDS             complement(8703..9650)
                     /locus_tag="ACG80L_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5023909.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACG80L_001681"
     CDS             complement(9873..10223)
                     /locus_tag="ACG80L_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5023910.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACG80L_001682"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACG80L_001682"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS5023911.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACG80L_001683"
     CDS             11646..11966
                     /locus_tag="ACG80L_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5023912.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACG80L_001684"
     CDS             11988..12452
                     /locus_tag="ACG80L_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5023913.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG80L_001685"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG80L_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS5023914.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="ACG80L_001686"
     CDS             complement(13301..14590)
                     /locus_tag="ACG80L_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS5023915.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG80L_001687"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG80L_001687"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS5023916.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4937 a         2914 c         3218 g         4648 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaag
//
LOCUS       DAZUMB010000063        15427 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000063 DAZUMB010000000
VERSION     DAZUMB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.064"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..2190
                     /locus_tag="ACG80L_001688"
     CDS             130..2190
                     /locus_tag="ACG80L_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS5023917.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2273..2842
                     /locus_tag="ACG80L_001689"
     CDS             2273..2842
                     /locus_tag="ACG80L_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5023918.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3017..3823)
                     /locus_tag="ACG80L_001690"
     CDS             complement(3017..3823)
                     /locus_tag="ACG80L_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023919.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3961..5475
                     /locus_tag="ACG80L_001691"
     CDS             3961..5475
                     /locus_tag="ACG80L_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS5023920.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5878..6861)
                     /locus_tag="ACG80L_001692"
     CDS             complement(5878..6861)
                     /locus_tag="ACG80L_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS5023921.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6866..7513)
                     /locus_tag="ACG80L_001693"
     CDS             complement(6866..7513)
                     /locus_tag="ACG80L_001693"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5023922.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7598..8230)
                     /locus_tag="ACG80L_001694"
     CDS             complement(7598..8230)
                     /locus_tag="ACG80L_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5023923.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8246..8797)
                     /gene="coaC"
                     /locus_tag="ACG80L_001695"
     CDS             complement(8246..8797)
                     /gene="coaC"
                     /locus_tag="ACG80L_001695"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS5023924.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8794..9531)
                     /locus_tag="ACG80L_001696"
     CDS             complement(8794..9531)
                     /locus_tag="ACG80L_001696"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS5023925.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9528..10517)
                     /locus_tag="ACG80L_001697"
     CDS             complement(9528..10517)
                     /locus_tag="ACG80L_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5023926.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10775..11317
                     /locus_tag="ACG80L_001698"
     CDS             10775..11317
                     /locus_tag="ACG80L_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS5023927.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11334..12419
                     /locus_tag="ACG80L_001699"
     CDS             11334..12419
                     /locus_tag="ACG80L_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023928.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12431..13237
                     /locus_tag="ACG80L_001700"
     CDS             12431..13237
                     /locus_tag="ACG80L_001700"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023929.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13238..14065
                     /locus_tag="ACG80L_001701"
     CDS             13238..14065
                     /locus_tag="ACG80L_001701"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023930.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14062..15135
                     /locus_tag="ACG80L_001702"
     CDS             14062..15135
                     /locus_tag="ACG80L_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5023931.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4965 a         2786 c         3138 g         4538 t
ORIGIN      
        1 aaacaataaa aaatcaaaaa aaatactctc ctaatagtcc atcttctttt gattttattt
       61 atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac
      121 taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta
      181 gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg
      241 atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc
      301 aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact
      361 ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt
      421 tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa
      481 accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat
      541 cagttaaaac agatcactga tattttgaat catgctttga cagatataaa ggacggtgga
      601 agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc
      661 gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca
      721 gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg
      781 actaatacag gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca
      841 atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat
      901 cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa
      961 agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca
     1021 actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca
     1081 gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat
     1141 tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat
     1201 cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatagattta
     1261 tcgacttatg aacaaaatgg gaaagtcact tctatttctg attggatgca agcaaacgga
     1321 ttagacagaa aaagtcactg gtcggagatc cttgaagtac ttcactcaga actgatgaat
     1381 aataggaagc aatcaggagg ggaaattacc tttacttatg tttatatacc agacaatcgt
     1441 tcattagaag gtcatgatct tattgttcat ccaaacgaaa aaataaacaa agaagagttg
     1501 atccaatcag caacaaattc cttaggtaaa cccgtaccgt taaaggatgt atcggttaca
     1561 atcaatgaag taccattgga ggatggttat ttgaccaaac aaacaggtga ttttacgatt
     1621 atgtatagtt atcatgaccc gtattcactc caagaactcc atgctcagtc actattacat
     1681 gttttaccat taaacaaaca gatagatcaa aaagaacaaa aaagacgaga agaaagcttg
     1741 tcagaaaaaa aacaacaaat ggctcatgca gtttctccaa cagggaaagt aaaacaaaca
     1801 agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa aacgataagg
     1861 caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt agagcctaaa
     1921 ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca gaaggaatca
     1981 gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat
     2041 ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg aggtattcct
     2101 tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata cgctaatcga
     2161 aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat agtcaaataa
     2221 gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa aaatgttaga
     2281 taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa atgttccaat
     2341 cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg aatctgcttg
     2401 ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac ttgagtattt
     2461 tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt ttgtagagac
     2521 aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa gagagcaagt
     2581 gtatccggtt tttcattggt tagcagtgaa accagattac caaggaaaag ggctcgcaaa
     2641 agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg gaccaatcta
     2701 tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa tgggttttac
     2761 ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag ttatgcaggt
     2821 tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt gggataaaaa
     2881 acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga taactaatgg
     2941 catgccatac attcaaaaaa gaaggggcag tgacaaaagt attgtcactg ccccttctct
     3001 tgttttttag catgttttat cgtttgattt ttttaggaat aaaattcttg atgaacgtaa
     3061 atttttggta atcgtttcga ctatagaggt aaccaaatac actgaaaaca actgctccaa
     3121 taaaattaac aaataaatcc ttcatagtat ccatgattcc cacgtctaag taaccatttt
     3181 ggatgattgt tttatctgtt ttaccatctg cattttgact ttcaatgatt gtccgagtga
     3241 tatctggaac atgatagaca gtgtttccgt ctttacttaa ctccacacta ttgatttgct
     3301 ggatgatcgt atctttttgc atatcgaatt gcagtaattt atctgctgaa aattccacaa
     3361 actcccacat aacaccaact gtcatcgaaa aacagaagga aacaactgct agtaataaag
     3421 gagtcaagtt catggttttt gtatttttat tgagcaaata aacaagggaa aagccgacac
     3481 ccccagctaa aaatccgctg aatgcatgta gaaaagtatc ccacattgaa aaatagccat
     3541 aaaaatcact taactctcct aaaactgttg aactaaaaat aaaagataac aaaatgattt
     3601 ctagcatatt tggaatctgt atctttaata tcttttccag aaaataaggg atttcaaata
     3661 aaattaatgt cagtaataaa agcattgtat ttccccagtg ttggttccag acctgatcca
     3721 ctagcataat aacgacgatg atacgtaata aaatatagat tccgtttgtg attctacctg
     3781 cagtttttgt actgttcgta aaaaaggttt tcaaatgatt cataaaataa ctccttcatg
     3841 tatatcctta attatactaa gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa
     3901 cagagttgat attgtgaaaa aaatcactta atatagtata aggagaaaag ggggatacaa
     3961 atgaaaaaat tagttgctgg tttattaaca gccgttgcac ttcttgctat gagcgcatgc
     4021 ggcgcagatc agaaaaatac agcgcagaca agttctagta cgaaggaatc tgccgaagta
     4081 tcatcaggcg cttctgaaca agtatataca gatccaaaag aaatgaaaga atcttatgat
     4141 gtcgtgatta ttggagctgg tggtgcaggg atggcagcag ctatcgaagc aaaagatgct
     4201 gggttgaatc cagtaatttt ggaaaagatg cctgttgccg gaggaaacac atctaaatca
     4261 tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag aagaaggaat caaagactcg
     4321 aatgatgcct tctatgaaga aacgctaaaa ggtggtggag gaacgaatga taaagaaatg
     4381 cttcgatact ttgtcgatca ctctgctgat gcggtcgatt ggctagatca aaacggtatc
     4441 aaactggaca acttaacgat cactggaggg atgagtgaaa agaggacaca tcgtccgtct
     4501 gatggctcgg ctattggtgg atacttagtg gaggggttat tgaataacgt ccatgagcgg
     4561 gaaatcccta tttttgtcaa cgcagatgtg acagacatta agaaaaatgg ggaaacggtc
     4621 aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc
     4681 gtagttacca caggcggttt tggtgccagc aaagaattta ttgaaaaata tcgtcccgat
     4741 ttagctgatt atattacgac aaatcaagaa ggatcaactg gagatggtat caccatgatt
     4801 gaaaaattag gtggacaaac cgttgatatg gataaaatac aaattcatcc aactgtgcag
     4861 caggatgagg gggtcttgat tggtgaggtt gttcgcggag aaggagcaat cttggtcgat
     4921 caagagggga aaagatttgt caatgaaatg gatacaaggg ataaagtatc tgcagcaatt
     4981 acagcattgc ctgaaaaatc agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc
     5041 acagctattg aattttatga ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa
     5101 ttagcagaaa aaatcgattt accaaaagaa acactagcaa aaacagttga tggatggaat
     5161 acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg ccacagccat ggaacatgac
     5221 ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag gaatccatta cacgatgggc
     5281 ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag aaaaccagcc aattaggggg
     5341 ttatatgctg ctggtgaagt cacaggtggc ttacatggaa acaaccggat cggtggaaat
     5401 tcagttgcgg agatcattat ttttgggcga caagcgggca tacaagcagc tcgatttgct
     5461 tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc
     5521 taataccgag ggtcagctga ttgaacactg tttagtgaag aggttgtgaa aaagctgccc
     5581 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggttgg
     5641 acttattacc gcagatgcaa gcttttgaac accgtttatt cggaaaaaga gcgtgggaca
     5701 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagaa
     5761 attccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct
     5821 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttgatcag catgatttta
     5881 ttcagttatc ttcaaaatga tttttccgat gtttttgttt gcttccatat attcatgcgc
     5941 ttgtttgact gcgctgaatt ggaatgtacg gtcaatgatt ggccgaatct tgccattttc
     6001 tagaaaaggc ccagcttttt gcataaaagt ttgtgcgagc tctgctttat actgatcact
     6061 tctaggtgtc aataatgtac caagcagttg gacacgtttc ccaatcaata caccaaggtc
     6121 tatctcaggc acgatggtcc cacctagcat accaatcaag acccaacgtc catcaacggc
     6181 tatagaagct aaattctttt cccagtaaga agcgccgatg aaatccaaaa taacatcgac
     6241 tccccgacct tcagtgattt ttcgtacctc ttcttgaaag tcttgttttt tatagttgat
     6301 caattcatct gcaccaagtt ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac
     6361 gatgattttc gcgtgagaaa gctgtctagc taattggatc gctgctgttc ccacaccact
     6421 tgctcctgca tggatcaaga cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt
     6481 ttgataagca gtcaaaaaaa cttctgagat tccagcagca gaaacatagt ctagcgtatc
     6541 agggagaagg attgcacgat cggcaggcat cacagcatat tcggcataac cacctagatt
     6601 gactaatccg tatacttttg tcccaggagt aaaagtggag atatcacttt tattctcaac
     6661 tacctctcca gctacttcga ctcccaaaat agggtaggga ggtttcaaag agactgattc
     6721 tcttttcata atatctgttc gattgacagc tgctgtatgg actttgatca gtaattcgcc
     6781 ttgttttggt ttaggaacag gcacttcaat ttgttctaat acatctacag aaccaggatg
     6841 cttgattgaa atggcacgca tggatctatt cctcctttgc ccattcttgg aatgctgtat
     6901 tcaactcttt gctaccttca gaaagtacta gagtcgtacg agaaatatcc gtttcttcca
     6961 ctagagcggc aagtacagca gcaactgtag agcgagtgat cgtttttgat gccttctcat
     7021 taggcttagt taattgaatg gaagtaactt cttcatcgtt agtcaatgtg actggttgaa
     7081 gaatgacaaa atctaagtta gttcgtttca gccattcatc tgcataatgt ttagtaatat
     7141 aataatctgt catggactca ggccattttg ctggttcatc tgcataaaca gcactgacca
     7201 tcagatagcg agagacatgc gcattttcag ctgcgatcac tgttttgatt gcgccatcca
     7261 agtctacttg cagaagattc tttccttgag agccggcagc aaaaatcaat acatcgatgc
     7321 ctttgaatgc ttcggtcatt ttttccacgg accaagttaa atcaaaagag acatacgaaa
     7381 cattctcttt ttttactaat gtttgggaag caacatctct cacccctgca aaaatttcat
     7441 gggaacttgg agctaaatct tttatcaggt gtcggccaat ttgtccgttt gcacctacaa
     7501 caaaaatttt catttggatc atcctttcga cgtcaaatct ttccattcaa tagtaacacc
     7561 aaaagagaaa aaaatcagag atcggctacg atgactatca gttagtaaga gaagggtgct
     7621 tgaagtatct taacaagaca ttgcatactg ctgcagtcgc aagacctgcg acgattcctt
     7681 caggtatacc acttgtaaaa ataaccgcta gaatagctga ataaacagca tttaaatttg
     7741 ttccgatgac tttggcataa tcttgctgga ataaaaagta gatcatattc atcactaaaa
     7801 tcgtattggt caatgaaccg gaaagtccag ctaagaataa agaaacggaa tctgcttttt
     7861 gtttcattag tttgcgcaga cctttataaa cgaaataagg aaccacccca atcaagatgc
     7921 gaggaataaa cgcgatcagc aaggctttcc agcttccatg ttctgttccg atgactggga
     7981 taaacggaga aaagacaaaa gacaaaggtg tttggataac tgtgctacgg atcatgctga
     8041 tcagtccaaa tgtaccgcca agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa
     8101 tgattacagg aatatgcatg gttgttgcat tgattggacc gatagggata aaacctaaag
     8161 gcgtaacagc taataaaatc aaaatagcta aaaacatcgc agttaatgta aagttttttg
     8221 tatttttcat aagtactcct tttttctaag catttctttc attaagagtc agcatgacct
     8281 gttcgatgat cgtctgaata tcagctaagg caccttttcc aaaatctcca caagctaaaa
     8341 gagattctct tggttcaatt tcctgatagc cgacagatct caatgtttcc agattacgct
     8401 gagtgatcgg gttttgatac atattcgtgt tcatggcagg ggcaatcaat tttggtgttc
     8461 ccactggaag cgccattgcg actgttgaaa gaagatcgtc tgcaatgccg tttgctagct
     8521 tgccgataat gtttgctgta gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt
     8581 cgatatgatt gattacctgc ggatttgatt cttgcatgac gtctgtatgg actgcgcgct
     8641 tcgacagaga ttgcaaggtt aatggcgtga taaaatgtgt gctgctttta gacataatca
     8701 catctacctg ggcacctaat ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag
     8761 aaatacttcc agtaacgcct agtaagatct ttttcatttt gattcctcca gtctaatttt
     8821 ctcagtaatc aatgcagcaa tctcagattt actttgagct tcttccactg ttccatcttt
     8881 agataagaga tagccgtggt gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat
     8941 aaaatcagtt ttgttattta tcagactatt ttttgcgact gccagaagag attcctttgg
     9001 tacatctact aatagtttga aaccaatgat ttttgctttt ggctgccatt cacgtagata
     9061 agaaatgatt tttggtgttt tcttcaaaac gagcacaaga tgctcggtgt tagaagagat
     9121 ctttgaaaca gtggtatctg tctgttcatt ttccgcaaac caattgtcca gttcttcttg
     9181 cgttgaagaa gtgggtaggt tttttgccag ctgttcttct gaaaaactaa aagcaggagt
     9241 aaaatcactg actgccatac tgtggataat cgcatcgtag tgttcttttt tcgttagttc
     9301 ttctaattgt aaaaataagt ccagtgcaga ttcgatcgta taaatcgtta tattggagtg
     9361 tcttttcggt ttcagtgctt gtttcgtagt cacgtaatcg attgtggtat ccggattggc
     9421 agctaaataa tcagctaatg cttgccctaa tcgtcctgtc gaatgatttg taatagcacg
     9481 tacttgatcg attttttcag tcgttcctcc agctgtgatt agaatcttca tttatggtca
     9541 cctacattta ctagtgttct tccttggtgt gttccagcaa gtagtttaga tgctgtttca
     9601 ggcaattgat ccagagtaat ctcttggacg accagttcat ctgcaatctg caaatccgcc
     9661 atctgttgcc ataaagaaag acgttgatct attggtacat tgacagaatc aattccaatc
     9721 aactggatgt ttcgcagaat aaaaggcaag acagtcgcgt ttagtttgat tcccccagca
     9781 ttgccacata aagctactgc accgttataa gagatcaatg gaagaagact gcttagctgc
     9841 tcgccaccta ctgtgtcgat gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc
     9901 tctggcagaa attcatcagg tcgtacgact tgcaaagcac ctagactttt tagccatgtg
     9961 acagactctt cttttctgga aaaggcaatg attgactgga atcctagttt atgtaacagg
    10021 gctatcgctg tactgcctac accgccagaa gcaccagtaa cgacaacttt tttatcggag
    10081 gtcatacctt gacggattaa agcgttcaca cacaacatag cagtaaaccc tgctgtacca
    10141 aggatcatcg cttgacgcag cgtcatattt tttggtagag gaactaacca gtctcctggt
    10201 actttttgat attggctata accgcctgga tgggtgacgc caagcccaaa accagtagca
    10261 agtacaagat cttcttttga aaaacgtgga ttgttacttt ggacgacagt tccggataaa
    10321 tcaatccctg gagtcatcgg atatgttcgt atcacctgac cagactcgct cattgccaat
    10381 gcatctttat aattgacatc agaataggca atttttacca agacttcatt ttcagccaac
    10441 tcagtaaatg gcagatcact gattgctgta tggaaaacag gttcctttat tacctgaaaa
    10501 gatcgaaaag cgttcattcg tgagacactc cttttaaaat tttcttttat cataccataa
    10561 agcctactct tttattcttg ttcttccgta aatttagaca aaaattaggt gaaaaataaa
    10621 gaatcaaaca actagaattt attccaaaag ataaagaaat attcagcaaa aaaaatttta
    10681 caaagggttg cctttttcaa taaatttcgt atactaatac gagtggtagt aaacaggaag
    10741 tttactatta gaaaacttcg attcggagga aattatggaa attggtgaga agttaagaaa
    10801 tttgcgcatc caaaagaatt tgacacaaga agaactaggc gaacgcacag atttgtcaaa
    10861 agggtatatt tcccagttgg aacgagattt aagttcacca tcaatggaaa cattcttttc
    10921 tattcttgaa gtcttaggtg tgactccaga agaatttttt catcaagaag cagcgaatct
    10981 gcaagtggtt tattcaaaag aagatcatac ggtttatttc gatgaagaaa acgggtatga
    11041 attggaatgg ctcgtggctg attcgaatga aaaagaaatg gagcctgtct tgctgacttt
    11101 tgaaggtgca ggagaatata agacgtttga accttcatta tctgaaacgt ttatttttgt
    11161 attagaaggg gaattagagt taagcttagg agagacggtc tatgtggcaa aagcaggaca
    11221 gtcgatctat tatcaagcga caaaacaaca tcaattaagg aatcatctca aaaaacgaac
    11281 aaaagtattg attgttgcaa cgcaatcata tttgtaatat agggggtaaa aacgtgggga
    11341 aaccaatcat ttcatttgat caagtagtga aacgctatga tgatgaaaca gtattaaaaa
    11401 aagtaagttt tgagatcgaa caaggaaaat tttatacact gcttggtcct tccggctgtg
    11461 gtaaaacaac gatcctacgt attatagctg gattcaccga agctacagaa ggggacatct
    11521 attttgaagg gaaacggttg aatgatttgc cagctaataa gcgacaagta aatacagtct
    11581 tccaagatta tgcacttttt cctcatatga atgtttttga taacgttgct tttggattga
    11641 agatcaagaa aatgtccaaa tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg
    11701 tccagcttcc tggatacgaa aaacgggaga ttagtgaaat gtctggtggt caaagacagc
    11761 gtgtagcgat tgcacgagca attgtaaatg aaccgaaagt attgctatta gatgaaccgt
    11821 tatcggcgct tgatttgaaa ctacgtacag atatgcagta tgaactgcgg gatctgcaac
    11881 aacgattagg aatcacgttt atttttgtta ctcatgacca agaagaagcg ttggcaatga
    11941 gtgatgagat ttttgtcatg aataaaggga aaatcgtcca aagtggtaca cccgtagata
    12001 tttatgatga gccaatcaat catttcgttg ctgattttgt tggggagagc aatatcgtgg
    12061 acggtgtaat gatcgaagat aatctagttg agtttgttgg taagcaattt gaatgtgtcg
    12121 atggcgggat gcgtctaaat gaaccagttg aagttgtttt acgcccagag gatttgacga
    12181 tcacgacacc agataaagga aaactagtcg taacggtcga tactcagctg tttcgtggtg
    12241 tccattatga aatcatctgt tatgatgaac agcaaaatga atggatggtc cattcaacga
    12301 aaaaagctaa agaaggaagc aaagtaggat tggcctttga gccagaagat atccatgtca
    12361 tgcgtttcaa cgagtctgaa gaagaattcg atgcacgtct tgacagctac gaagaatagg
    12421 ggggacaaga atgaaaacaa tgcgcaggat ctattccgtc ccttatattt tgtggctcgg
    12481 tttattcgtg atcgcgcctg tattaatgat catttatcaa tctttttttg atatgaatgg
    12541 gcaattcacg ctgaataatt atctatctta ttttacgtcg gggacatcgc tttctatgac
    12601 gatcaattct gtatggtatg catttttgat cacgtttttc acgctgatca tcagttaccc
    12661 gactgcttat tttttaacaa aactgaagca caagcaatta tggctgatgc tgattatttt
    12721 gcctacatgg gtgaatctgc tgcttaaagc ttacgcattt attgggattt tcagtgtgca
    12781 cggcagcgtg aatcatttct ttgattttat cgggatcggg ccgaaacaga tattattcac
    12841 agacttcagt tttctgttcg tggcaacgta tatcgagatt ccatttatga ttatgccaat
    12901 tttcaatgca ttggaagaga tgaatccttc tttgatcagt gctagtagag atttaggcgc
    12961 gaatagcttt gagaccttcc gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag
    13021 tggtgtccaa gcagtattta ttccttctct ttctttattc atgctgactc gtctgattgg
    13081 cggaaatcgg gtgatcactt taggaacggc tatcgaacag catttcttgg ttacacaaaa
    13141 ctgggggatg ggttctacaa tcggtgtgat tttaatcatt gctatgttca tcgtgatgct
    13201 cctgacaggc gagaagaaaa aaggaggtcg ctcataaatg aaaaaattta aatggtcgta
    13261 tctttattta gggatcgtct ttttattctt atatgcaccg atcttctatc tgatttttta
    13321 ttccttcaat gaaggaggaa cgatgaacag ctttacagga tttacttggg aaaattatga
    13381 agctgttttt gaagatacac gtttgatcat tatcgtattg aatacgttca tgctggcttt
    13441 cttatctgca ttgatcgcca cgatcatcgg gacatttggg gctatgggaa tctattacac
    13501 gaaaaaacga cgtgcacgga cagcgttgtt gagcatgaat aacatcttat tagtctctcc
    13561 tgatgttatt atcggtgcta gtttcttgat tttctttaca ctactaggat tcggattagg
    13621 ttttcagagt gtattgcttt cccatattgc tttcagcatt ccaatcgtcg tcttgatggt
    13681 cttgccaaaa ttgcaggaaa tgaacgattc aatggtcgat gcggctagag accttggtgc
    13741 taataatttc caagtagtca aaaatatcat tttgccttat ctttctccag gaatcatagc
    13801 tggatatttt atggcattta cctattctct tgatgatttt gcggttacat ttttcgtcac
    13861 aggaaatggc tttacgacgc tctcagtaga gatctattct cgtgcaagac aaggaatcag
    13921 tttagaaatc aatgcgctaa gtgcgttagt tttcttgttc tccatgatat tggtcattgg
    13981 atactacttt attagtaaag aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg
    14041 cgagggggtg gcacggctgc gatgaaaaaa ttacaatctc tgtttttagg tattctcatc
    14101 attattgcgg tgctgctgct tggtgttcgt caacttgaaa aaacaagtgg catgagtgga
    14161 gcaaaggtac ttaccatcta taactgggga gattatatcg atcctagttt gatcacgaaa
    14221 tttgaaaaag aatatggata caaagtgaat tatgaaacat ttgattctaa tgaagcgatg
    14281 ttcacaaaaa tccagcaagg tgggacaaac tacgacatcg ctataccaag tgaatatatg
    14341 atccaaaaaa tgatcaaaga aaaactagta cttccattag atcattcgaa gatcaaagga
    14401 ttaaataata ttgatccacg atttttagac ttggattttg atccagataa tcagtattcg
    14461 attccatatt tttggggaac tttagggatc gtatataacg ataaattctt tgatgcaaat
    14521 caaattaagc attgggatga tttgtggaag ccggaattaa aagacagttt gatgctgatt
    14581 gacggtgcac gagaagttat ggggctttct ttgaatagtt taggctattc tttgaatagt
    14641 aagaatatgg cacagttgac agaagcagca aataaattga atcatttaac accaaatgtc
    14701 aaagcaatcg tggcagatga gatcaaaatg tatatgatca atgaagaagc ctccgtggca
    14761 gtgacatttt ctggcgaagc tgccgatatg atgtgggaaa atgaacattt acattatgtg
    14821 atcccaccag aaggatccaa tctttggttt gataatatcg ttatgccaaa aaccgtgaaa
    14881 aataaagaag gtgcctatga tttcatcaac tttatgctag aaccggaaaa tgcggcacaa
    14941 aatgcagagt atattggtta ttctacacca aatgaagaag caaaagcgtt attgccaaaa
    15001 gacatttcgt cggacgaaca attctatcca tctgatgaca cgatctccca tcttgaagtc
    15061 tacaaagatc taggatcgaa atacctaggc atctataatg acctattctt agaattcaag
    15121 atgtatagga gataatggca aaactcagta agaatgggga cttatcaacg ttctaaagag
    15181 ctaaaaatcg tcctttttct atctgagttt tccaatcttc taccaaaagc ggatgaaacg
    15241 ccgctttttt tgtgttattt tattttttct accacgtttt tctaccagag aaaagtggac
    15301 aaaaccattg taaaataggg cccgagacgg gctagtaaat ctaagattga aaaagttaca
    15361 gaaggataaa gtaattggaa actatgcaaa tatacttaac ccttctgtaa tctattaata
    15421 aaactaa
//
LOCUS       DAZUMB010000064        15072 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000064 DAZUMB010000000
VERSION     DAZUMB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.065"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..170
                     /locus_tag="ACG80L_001703"
     CDS             <1..170
                     /locus_tag="ACG80L_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5023932.1"
                     /translation="YQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRAR
                     IYLQQGLIFEN"
     gene            complement(553..1281)
                     /locus_tag="ACG80L_001704"
     CDS             complement(553..1281)
                     /locus_tag="ACG80L_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5023933.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1259..2833)
                     /locus_tag="ACG80L_001705"
     CDS             complement(1259..2833)
                     /locus_tag="ACG80L_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5023934.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3062..4000
                     /locus_tag="ACG80L_001706"
     CDS             3062..4000
                     /locus_tag="ACG80L_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023935.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4012..4923
                     /locus_tag="ACG80L_001707"
     CDS             4012..4923
                     /locus_tag="ACG80L_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5023936.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4947..6419
                     /locus_tag="ACG80L_001708"
     CDS             4947..6419
                     /locus_tag="ACG80L_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5023937.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6470..7081
                     /locus_tag="ACG80L_001709"
     CDS             6470..7081
                     /locus_tag="ACG80L_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5023938.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7111..9024
                     /locus_tag="ACG80L_001710"
     CDS             7111..9024
                     /locus_tag="ACG80L_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS5023939.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9152..12133
                     /locus_tag="ACG80L_001711"
     CDS             9152..12133
                     /locus_tag="ACG80L_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS5023940.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12130..13791
                     /locus_tag="ACG80L_001712"
     CDS             12130..13791
                     /locus_tag="ACG80L_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS5023941.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13831..14763
                     /locus_tag="ACG80L_001713"
     CDS             13831..14763
                     /locus_tag="ACG80L_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5023942.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4894 a         2389 c         3030 g         4759 t
ORIGIN      
        1 gctaccaatc gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg
       61 ggctgaataa caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata
      121 actttcgggc acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc
      181 ggcgcacaga acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac
      241 gatttgacga agagcctatt taaataacta catccattta ttctatcttc taaaatagcc
      301 tttttcattt cagttaaata gttcagcgcc atttttgcac tcgacttgca tagtagtttt
      361 ttccatattc ctcaacctca gcgggggttg aagacataag gaaaatccta tgcgttatgc
      421 tcaactggat ctgattaaat attctaatgt atttttctct accatcatta aatggttaat
      481 agccaaaaat caccactttt caatggctcc ttgttgtata agtataacta gaagatgaac
      541 acgtctgaga aattatagaa gctttttacg gtaattactg gggctgtgtc cagtagtttt
      601 tttgaagaga acagaaaaat agctcacgtg atctattcct actttttgac taatttcatc
      661 aatagataag aatgtgtctt tcagtagagc acttgctctt ttgattcgaa agtctagcaa
      721 gtaatcatga aaggaataac ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg
      781 gtaatggaat tgtgtagcta catctttgag cgtaagcttt tcactataat tgttttgaac
      841 gtaatcaatc acttgttgaa ttgactcatt aaaagaatca tcagttagag agttttgatt
      901 agctgctgat ttacgcattt tttcttcttg tactaactta actaaataag gaaaatcaat
      961 gggtttcgtt atgtaacttt taatagataa atcaatcccc ttttttgcat actgaaaatt
     1021 ttggtgggca ctaatgacaa tactagtaaa gtagcgattg gatgtttgtt gcaaagattc
     1081 gattaattcg agtccagaaa ttttccccat cgtaatatct gtgataagta aatcaaagcg
     1141 ctggttttgc aggtttttaa atgcttcgtc tccgtctgtg tagcagtgat cgataatgat
     1201 gtcggggact ttcttcaaaa gataatgcct tatgccttct acaattgtac aatcatcgtc
     1261 aacaattgca attctcaaca tgcatattcc cccctttttg tattttgata gtaatcgttg
     1321 ttccgacgtg tagttggctt tctataacaa aacgtgtatc tccaggatag agatgcttta
     1381 tacgtcgaaa agaatttgag atgccgatat tctcactata aggaatatca tcattaagtt
     1441 tttgttgaat atgctccagc ttgtcttggt ccattccttt cccattttca acgattttta
     1501 acacataatg gttggttgtt tcagttgcat agacagtcaa acgattttcc ttttttgatg
     1561 aatcgaagcc gtgtttaata taattttcaa caaatggctg taaagaaagt tttggtaaca
     1621 ttaattgatc aatatgatca atcagctgat agtcaaagtt atgtcgataa cgacgttgat
     1681 gtagttgcaa ggagaggtcg atattaatta attcctcttt caaatcaatt atgtcgggct
     1741 gatatgtatt attgcgatac aacttgctca ataaatatat actgtcgctg atttcttcgt
     1801 catcattcaa tatggcagtc attcgaataa attccaaatt attaaataga aaatgaggct
     1861 cgatttgata ttgaagtgct gcatactttg cttcttgctt caaccaatta ttttcataaa
     1921 gtgttttttg atatgtagca ttatctaaat aaaactgatt gatatgcata gcgatttctt
     1981 tcaaatcacc gactttgttt tcgatattga ttgacttgta gtctcctgta gatgaatgat
     2041 ttaaggaagt aatgatttcc tgatactgct ggatatagtt acgaaaactt ctatttaata
     2101 attgatgaat agcaagaata aacaaactca ttaataagaa aatcatgatt gccagaccta
     2161 ataaggtttg tgacgaataa tttggatttt catatacttg taatgtgtgg ttaccgatag
     2221 ctatctgttg cgtttttttc catttagaag gcgagatgtc ttcagataaa ttgacggaat
     2281 caataccatt agacaaagaa taattgtaat gtgttggaaa gagactcttt tttagcgcgt
     2341 tgatcgaaac gtctagaatc aatgtagcat cgggcatgtc ctcactaatg aaacttaatg
     2401 aacgaataaa agaaattttt tcgttttttg taggtgcgta ggcacttaag tatttttcgt
     2461 aatctaatga gatagatata tttttaactt catcatcttg cacaagtttt tttaagttat
     2521 ctactaaata ggtaggggag cgatcctttg acaaaatatc ttccatgtat tcagcaggtg
     2581 atgagaggta gtagtcatta aggttttctt ggaaatcata attttgatag atctctgtca
     2641 cgataccgga gagaatacga ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt
     2701 gcaggttttg atttttttct ttttcaatca cattgatata ggaaaagaac aatgtgaaac
     2761 aggcaagtaa agtaataaaa aaaatcaata tgtatgtaaa acgcataata acttttgata
     2821 aatcgttttt cattcaaagt cctcattttg ataatctcta attttatgaa tcattatagc
     2881 acatattaca agtgatgtaa acgctttgca aacagggcta cttatgattg tgaatacagt
     2941 ttccgtttaa aaactattct attgtagagg tttaataaac taaaaataat gtataaaaaa
     3001 ttaatgtaag cgattaacca agtgggtata atcaaggaaa cgctgagaga ggagagacaa
     3061 aatgaagcta aagaaagggt tattaggaaa tctaaaaaaa aaccgagcat tgtttattat
     3121 gcttttacca ggtgcgttat ggttatttat attttgctat ataccaatgt ttggagttgt
     3181 aatcgctttt aaagatttca gaatttcatc aaggggattc tttacaagtt tgttcaacag
     3241 tccttgggtt ggctgggaaa atttcaaata tctctttgct tcgcaagatg cttggatcat
     3301 tactcgcaat acggttgttt ataacgttgt aggtattttg ctaagtactt tttgtacagt
     3361 gtcattagca atcattctga gccaaatggc gaacaaacgg ttattgaaaa agatgcagac
     3421 atttatgatt tttcctaatt ttttatcatg ggtagtcatc agttactttg tttacgcatt
     3481 tttgagtacg gataaaggat taattaatat tttactaagc aattttggtg tggaacccat
     3541 aaattggtat agtgaaccga agtactggcc ttttattatt gtttttacga gtttatggaa
     3601 aggcgtaggc tatggatcca tcatttatta cgcgtcaatt atggggatcg atactagtta
     3661 ttatgaagct gctttagttg atggtgccag taaatggcaa cagataagga atgtagtctt
     3721 gccacattta agatcgatta ttgtcattat gttgattttg tctgttggta gtatttttcg
     3781 agctgatttt ggattgtttt atcaattgcc aagaaattcg ggggctcttt atgatgtgac
     3841 gaatgtatta gatacttatg tctaccgtgg attaatgaat agcggagata tcggaatgtc
     3901 tacggcagcg ggattatatc agtcatcagt tggctttgtc ttgattttga ttactaataa
     3961 gatagtcaac aagttagatc ctagttcagc actattttag gaggaatcaa aatgatatca
     4021 aaattaaaac ggcagatgta cttttcgctg ccagtcgaac ggtttttcac aatattacta
     4081 atcttattta ctcttagctg tatttttccc tttattttcg taatcgtcat ttcattaaca
     4141 gatgaaaaag cattacaact caacggttac gaattattgc cggctcaatg gagcactgat
     4201 gcatatcaat acttaataca agatggcgga cagttacttc ggtcattagg cgttacaatt
     4261 atgattacag tgattggaac gttgattact gtttttatga cgggaaccta tgcttatgtt
     4321 ttatcaagag cctctttccc ttaccggaag ttctttactt tttatctttt ttttaccatg
     4381 ttatttgcgg gagggatggt tccttcttac ttagtgatga cgaagatgct tggcttaaaa
     4441 aatacaattt gggctttgat tctacctctt gcttttagcc catacaatgt cattatttta
     4501 aggacattct ttaagaagtc gattccagaa tcgattatcg aatccgctaa aatggatggc
     4561 tgtagtgagt ttcgagtttt tttccaaatc gttttgcctt tagcgatccc aggggttgca
     4621 acaataggtt tattttcaag cttaggttat tggaatgact ggttcaacgc attgctgtat
     4681 attgacacca acaaattgat tccattgcaa tatttgttaa tgaagattca aaattcaatg
     4741 gaattcttag caaataataa tgacattaca ttagcgcagc aacaagcaat tcaaaatagc
     4801 cttcctcaag aatcaacacg aatggcaatg gtcgtagtag ctactttacc gattgcaatt
     4861 gtttatccgt tcttccaaaa atactttgtt caaggattga cgatcggtgg agtgaaagaa
     4921 taaaaaagaa taggagatgt taaaagatga aaaagaacat gcagaaatat ttgattggtg
     4981 gtgcttctgt actaaccctt ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa
     5041 ataatgaaac aggaaccaca aaagttactt ggtatcaggt tggagaagaa cccaaagatt
     5101 ttgatgaggt catgacaaaa gtcaacgaaa aattaaagga agaaatcaat gtagagttgg
     5161 atatgcggct gattccggac ggggattatc agcaaaagct tggtgtgatg atcaattcag
     5221 gagaagaata tgatatttgt ttcgtcaatg gcacagatta tgtgaattat gggaacaaag
     5281 gtgctttcat tagtctcaac gatcatttgg ataaagagtt gaaagattat gcagctgaat
     5341 tgaatgaagg atttattgaa ggtggggcaa ttaatggcga aacttatgga ttgccagtca
     5401 ataacgatat tgccacaacg gaatattgga cctttaataa agatttggta gatcaatacc
     5461 aattggatgt agagaatgtt gaaggtgtag aaacattggc tccagtatta gaaaagtttg
     5521 cagaaaaaaa tactgatacg aatattacgc caatcggtat gacccgtacc agtgtctgta
     5581 ttccttatga caatattatc ggtggtggtg taccgctagc ggttgcattt gaaggcgata
     5641 cacaaacagt tgttaatact tatgagactc cagaaatgat gacttatttg aaggctatgc
     5701 atgagtttta taaaaaagga tatattttga aagatgcagc cactgttacg acagatccat
     5761 ttcgaattga tagtaaaaac tggtttttag gtcgtaactc aagtggttat caaccattag
     5821 cacaaaaaaa attagaagct ctagcgggac atggattaat tttaaaacca gctggaaaag
     5881 cttttaaaac gactggattt gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac
     5941 atccggaagc agccttgaaa ttgttgaatt atgtgaatac caatcaagaa gtagcggatt
     6001 taatggttta tggaattgaa ggaaaacact atgatcgcaa tgaagatggt tcgattactt
     6061 taacaaactc tgatgattat tatcaaggcg cttgggcatt ggtaaatact tatctagttt
     6121 caaaaacttc accgattaca gaagaaagta aaaaactaga agaagaaagt aaaaagtttc
     6181 aaaatgaagc accgatgtct cctattttag gtttctcttt tgacgcttct gattttcgaa
     6241 cagaaattgc aagtgtacaa aatatcgtgt cacaatatga agtactttta agctcgggaa
     6301 cagttgaccc tgaacaatat gtaccagagt tgttagaaaa actagaaaaa gctggattgg
     6361 ataaaattca ggcatctgct caagaacaat acgatgaatg gcgaaaatct aacaaataat
     6421 tttttactaa atgaaaagaa tagactaagc agttagtggg gggattcaaa tgaacgaaaa
     6481 caaatggacc aaaggattta attgggtttt tcaattgttg ttactaaact ttacttattt
     6541 tatactaatt tgtttaggat ttggaatttt aggttttttc cctgctacga tagctttgat
     6601 tgctggcgtt gtgaatcgaa aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa
     6661 aatctataag aaaaattttg ttagatataa tctatattgc tggtcagcaa ctttgttgtt
     6721 ggccggcaat attactacga tctggctttg ttggcagcaa agcgcaccat tgttttattt
     6781 attaagtaca ttattatcgg taattacact gttcctagtc tttgcaatgt tgaattgggt
     6841 cgtgatcaaa agtcttgggt tgtggatgat gggagaccga ctgattgact atttgctttt
     6901 cacagcaacg atgttgccaa cggtagtcat gcaaggcttg gtgcttggaa tttatttccg
     6961 aatattgatg attttgcctg tgatttttct gtttggcgga acagctgtaa cggtaatatt
     7021 gattagtaaa ttaactgaaa gagcgttagc aaagttgcct tgtcagatac aatcgcacta
     7081 attttttcac aaaaaggagt atttctttct atgaagttaa ctgataaaca agtagctaat
     7141 atagccttta tccagcaact tgagattcca ttgacacaaa aagatgaaga acaaatagac
     7201 ttgattttta ctgacacaaa ggctaatgtg cgacaaatca agattactaa aaatgagcag
     7261 caacgaattt gcttgtctta ttcacatgaa gacttgctgt atcgaggatt gtttatctgc
     7321 cttccacgtt ttttagctca aggaggagag atgccataca ttgaagaaac ctcgatcatt
     7381 gagaccagtt attcattaga tgcttctcgt aatggcgtga tgaataaaga gactctaaaa
     7441 aaattcattt cttacttagc gttgatgggg tacaacacat tgtatattta cacagaagat
     7501 acctatgaag tagaagacct tccatatttt ggttaccttc gcggagcgta tacaaaagaa
     7561 gaagtgaatg aaattgttga atttgcttct ggttttggca tagaagtagt tccatcgatc
     7621 cagacccttg ctcacttgac ccaatttttg aagtggtatg ctcaacaaga actgatggat
     7681 gatcaaaata ctctactgat cggtgaagaa aaggtttatg cggcaatcga aaagatgatt
     7741 attgcctgca aagaaatgta tcagacagag cgtattcatt tgggaatgga tgaagcctat
     7801 caagcaggtc tgggacgata tttaagcctg tatgggtatt gtgatcggac aactttaatg
     7861 attcaacata tggaccgtgt gttatcaatt gttgataaat ataagatgaa accaatgatt
     7921 tggagtgatt tcatttataa gctattagat gaaaccggta aagataggta ttacaatcct
     7981 gattttaaac tcaaagcatt agcagctaaa ggattaccag caggattaac ctatgttcat
     8041 tgggactatg gttcagatga agtttcctcc tatgagaaag taattgataa ccatttgaaa
     8101 ttttgtgaca ttgatcatta cgttttagct agtggtgccc atatctgggg gagactagcc
     8161 ccgaatcatg ggaaaacaga agcaattgtt tctgcagctg taaaagcttc taaaaataaa
     8221 ggtgtacgtt ctgtgatgtt aactacttgg ggagatgaag ggcaggaaac agagcattgg
     8281 cattccttaa ttggggcatt aaaattagca gattatgtct attctgaaga agagtggaat
     8341 gattttgagg aaatctacga tcgtttatta ggaacaggta gttatcaatt aatgtccgaa
     8401 ttacgatatt tcgatgaagt ggataccatt acaaataaca acttacaaat ggcttccgtg
     8461 agtaaactgc tactttggca agatccgtta gttggtatat atgactttca tgtagccgct
     8521 cataacaaaa ttgccactag aacaattggt acttattatc atgagttagc tcagaaatta
     8581 gatatgaata aaaattggaa tccaaaacaa ccaattttaa caactattcg tcaacgatat
     8641 atacttttag ctgaaatttt agcacaaaag gcagaactag gcatctattt acaacactgc
     8701 tgtctggtag aagaccttgc aggattaatg gaattagcga atcggtgtga agcactagcc
     8761 aatcgagtaa atgaattggc atctattcat gaaataattt ggtatcaaac atacaaaacc
     8821 tatggttggg aagtattaga acaacgatat gctgcattag ctcatcgtct gaaaaccgtt
     8881 gcgcgtcgta ttaaaaatca tcttcagggg caagatgccc tagaggagct tttggaagaa
     8941 cgattaccgt tttgtcaata tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa
     9001 agctcggtgt caggctacaa ttaacgaagg aatcttcccg ttgaacggac cacttgctca
     9061 tctaatggga aagtggttcg ttttctattc ataagtagct taccactaac tcttttatta
     9121 agaagagaaa aaaagtagga agtgagaaaa tatgttatcg gactattatc aaaatctgtc
     9181 agttataagt gtcaatacag aaccattacg taattattat attccttatg caagtaaaga
     9241 agaagcaaaa caagagttag aaagggaaaa aacgagccag tttatcgatc tcaatggtac
     9301 atgggatttt cagtattatc cttctgtcca acagttaccg gaaaattttt ttcaagaggc
     9361 ctttactgaa agctatgata gaatttctgt tcctggagtt tggcaaacag agggatttga
     9421 cttacctcag tatatcaatg tatcttatcc aattcctttt gatccgcctt atgtacctaa
     9481 tgatacccct tgtggtgtat atcggcgtca gtttcaaatt gagcaaacag atgcacgctt
     9541 ttacttaaat ctagaaggag cagatagtaa ttgttttgtt tggataaata gaaactttgt
     9601 aggctatgac caagtgagtc atagtacatc tgaatttgat gtgaccaagt atttacaggt
     9661 tggggttaat gaaattcaaa ttctagtgat gaagtggtgt gatggtacct actttgaaga
     9721 tcaggataag tggcgaatga gcggtctatt tcgtccagtg tatcttcttc aacgaccaac
     9781 tatgcatctg acaagttatc aactacggac gttgactgat cctaaaaaag gaagcggaac
     9841 attaatggtt tcttttcaac gatttactcc tattgaagta tcgttaccta tttcttacga
     9901 attattccat cctgatggac gtctcatagc gaaaggggag tgttactcgg aacaacaaat
     9961 aaatttggat gttgaagatt gccttttttg gacagcagaa acgcctaaat tatatacatt
    10021 acttttaaca tgtaatggtg aatattttaa agaaaaagtt ggctttcgag cactgacgat
    10081 caagaatcgt aagttattgc tgaacggaac acctattaaa ttatatggag tcaactatca
    10141 tgacgtgaat cagcaaggca cgccagtgat gacccgagag cagttacgtg cagatttgtt
    10201 attaatgaaa aaattaaatg tgaatactgt acgaacggca cattacccta aatcaccaga
    10261 tttttatgaa ttggcggatg agattggtct gtatgtaatc aatgaagcag atttggagtg
    10321 tcatggtgtg gttaatctat atgatactga agcgaattat caactaattg ctaatgatcc
    10381 gacattcgaa aagccgatta tggaccgagt gatccgttct attcttcctt atcaaaatcg
    10441 aagctctatc attatgtggt ctatggggaa tgaatctggt tatggagtaa attttgaaaa
    10501 agctcaagct ttagcacgga aattggatga ttcacggttg attcataatg aacgctcgat
    10561 tgaaccagtt attgaccaag tgaatgattt tagtaacttg gatgttatta gtcaaatgta
    10621 tcccccactg gaagatgttc atcgtcattg tcaaagaagt gatactgaaa aacctttttt
    10681 tatgtgtgaa tatagccacg ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga
    10741 tttgattcaa cagtatgatt cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc
    10801 aatcaataga ggaactttag aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa
    10861 atatcacgac ggcaattttt gcgtagatgg tttattgacc ccagatcggc aattaacagt
    10921 aggtgcattg gagtaccgta atattcattc gcctattcaa ctgattgctg tagatgagga
    10981 gctgcaagaa gtaaccttga gcaatataac tgcttttaca gccggttcag catttaacct
    11041 ttctcttgaa tggtatatag atggaaaaaa aacagtttat gaagtaatga atcctttcat
    11101 acttgagcct ggcaaaagtt gttcattgcc catcacagta cctacaacga ttcatggtgc
    11161 tcatacggtt gctgtttcgc tatctgttta taaaaatgat gagctttatg gacagcaaca
    11221 gctctttctt aaaaaagaaa aaatgagatt gccaagaact gctcatcata atgatgcacc
    11281 acaaatttca gtaaaaggca cagagctgat aatcaaagga gaatccttta tttatatata
    11341 tcaattagca aaagctgcgt tcacttctgt aaagatcaat caaaaggaac ttttggaacg
    11401 tcctactcaa ttttcaattt ggcgtgcacc aacggataat gatcgtaact tagtaaacaa
    11461 atggaaagca gctggatatg ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt
    11521 cgtagatgat catctcctga tcgaaacgtc ttatcgagga gttattccag gattacagcc
    11581 aatttttaca atgcacatac agttgaaaat aaatggtgca ggcgtaatca accaaaaagt
    11641 atcagtggag aaaaatccga tttttccaga tttccctcgc tttgggatgg actggccgtt
    11701 gaatgatact ttttcgaagg tttcttattg tggctatggg ccgtatgaaa gttatcctga
    11761 taagcactat gctgcgatgt ttggtattta tgagatggag attgaggaat ttgatcagcc
    11821 atatatcaaa ccacaagaat atggtaaccg ctggcaatgt gattgggtat caatcaaaaa
    11881 tcatcaagga tctaccatga cggtttatgg aaactctaat ttttctgctc tgccttatag
    11941 tcaagctcaa ttaaccgaaa agtcacacca ttatgaatta ataaaagaaa atagaacttg
    12001 gttcaattta agttataaac aaaatggtat cggctcacac agttgcggtc cttatttaga
    12061 agatcgtttc cgttttatgg aagatacttt tgactggaat atagtgatta ctttcaaagg
    12121 agaaaataga tgaaagaaac aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa
    12181 gaagttgttt atcgaaacca ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt
    12241 aatggattat tttatcgttt cgaacagtta gaagaagtct ggaaaccggt aacgaatttc
    12301 caatcatgtc ctgtacagct cataaatgat ccaaaagaaa catacgattt atgtataaca
    12361 cacctttata accagcattt gaaaaatgac ggattttatt taaaggttac taatgacaat
    12421 caacttacga ttgaatcagc aaaccttcga gggtttcaat atgctatgga agccttatta
    12481 gagagttttt tttcggtagg agatcaatta ttattaccgg aattgattct gaaacatgaa
    12541 ccatcagtga aaattcgagg aatcattgaa gggttttatg gtatcccctg gacacatgaa
    12601 atgcgcctct ctcttttttc atatatgaaa gataaccaat tgaatacttt tatgtatgcc
    12661 cctaaagatg atgaattatt acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta
    12721 gacaaattta aggagctttt atctgctgct gaggctagca atatcgattt ttggtactta
    12781 attagtcctg gaaatgatat tgatattacg tgtgaagaag acattcaagt tcttctgaaa
    12841 aagttagaac aactgattga gcttggcgta tttcaatttg gtctattaat ggatgatatt
    12901 gactatcaat taaaaggtgc tgctaaacgc cgttttcgag agccagcatt cgctcatgcc
    12961 tatttagtga atcgagtaga ggaatatctt agcactcggt tgtccaatca tgaattagtc
    13021 atttgtccta cagaatatga taatcgtcat ggttctcgct atttagcaac gttaagtcaa
    13081 gaaataccag aacaattacc gttcttttgg actggcccaa gcacgttagc ggcgtcaatt
    13141 tctacagaag agctgcagga aatggcttct gtctatcaac ggccgatgct gatttgggat
    13201 aatattccgg taaatgatta tttagaggat aaagaactat tgttcatgag tccatatgag
    13261 aatcgaacac cgaatctttc taaggagaga taccaggtta caggagtagt ttcgaatcca
    13321 atggctcaat tagaagcttc aaagtttacg atcaatagta tggctaatta cttatggaac
    13381 tgtgagcgtt ttgatccttt ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt
    13441 aaactacaac cagcttatct taccttaacc aatgcttttc caaatcatta cacaagcagt
    13501 ctgctttcag atgaagaatt actgctagcc aaagacccag aatgggtcac aaaagaaatg
    13561 gctgctttag tccaagcagt aaaatggatc cagcaagaac aacatacatc tagattgaga
    13621 acagaattgg agccttggtt acaagcaatc acagaaagtt ggactttctg gcaagaatgg
    13681 caattaaaaa aagatccaaa agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc
    13741 ataggacgtg atttagtaac tgcccattta gagcaaatta tcaaaagtta gatatcttaa
    13801 aaaataaaaa gcaagaaaaa ggagaacaaa atggtcaaga gattaattag tgcaaactac
    13861 agtgatgtca agaaaatgac gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga
    13921 cgggtgattg tttgtgaaac ggttgctaat actcttccac aatctggaga aattacgaat
    13981 gcagaagttg cgtcagcatt tggtgcagat ttgattcttt taaatggttt cgattgttat
    14041 cgtcctgcag tttttggact accaaatgtg acgattgaga aggtttttga tctacagcaa
    14101 acgattgaaa acccgattcc taaattaaaa gaactagtcg gtcgtcctgt gggaattaat
    14161 ttagaaccca ttgcccctaa aacaaatatg atgagtgaga aagtagaaat cagccaagga
    14221 agagcaagct ctgttgaaac gatacaaaaa gcagaagaac taggagtcga ttttatttgc
    14281 tttaccggta atccaggcac tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg
    14341 gcaaaaaagt atttttcggg attgattatt gctgggaaaa tgcatagcgc cggctcagaa
    14401 gagccagttg tgaataaaga ggcgattatt tcttatgcgg aagcaggcgc agatatttta
    14461 ctagttcctt cagtagggtc tatcccaggc tttttagagc aagacctaat tgaagcggtt
    14521 cggttggcaa aaaaatatga tttgttaacg atgtctgcaa tcggtactag tcaggaaagt
    14581 gcccgaccag aaacattgcg gcaattagcc attatgaata aaagttgtgg tgttgattta
    14641 caacatattg gagatgctgg atacggggga ttggcaccag cagaaaatat tttcgaaacc
    14701 tcggttgcaa ttagaggaat gcgacatacc atcaactgcg ttagccgctc gatcaatcgc
    14761 taacaaaatt aaataaaaaa tggttaaatt gattatttaa gtgggaacat acttgagata
    14821 tcagctaact tctgtcactt taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa
    14881 tatccgaaca agagactagg atagcagaaa aactatcgtt ttgcaacgag gaccaactgg
    14941 accgtaggag caattctctt attgttcgga tatttcgctt gttcaaggac ttatgtctgt
    15001 agtgcccttt tttggtagga aaaatggtag ggaaccatat catttttaat gaaaatcaag
    15061 catttcaaaa gt
//
LOCUS       DAZUMB010000065        14692 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000065 DAZUMB010000000
VERSION     DAZUMB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.066"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..698)
                     /locus_tag="ACG80L_001714"
     CDS             complement(<1..698)
                     /locus_tag="ACG80L_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5023943.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAV"
     gene            873..2297
                     /locus_tag="ACG80L_001715"
     CDS             873..2297
                     /locus_tag="ACG80L_001715"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5023944.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2596..3303
                     /locus_tag="ACG80L_001716"
     CDS             2596..3303
                     /locus_tag="ACG80L_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5023945.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKQAFF"
     gene            3260..3613
                     /locus_tag="ACG80L_001717"
     CDS             3260..3613
                     /locus_tag="ACG80L_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5023946.1"
                     /translation="MGADWGCGSNRPSFEFTLLTILGCTLATWLSRILPFVLLKKFDL
                     PQPLLEYLSFVPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLV
                     IVLVGILSLSLIRLL"
     gene            complement(3702..4739)
                     /locus_tag="ACG80L_001718"
     CDS             complement(3702..4739)
                     /locus_tag="ACG80L_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5023947.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5060..6043)
                     /locus_tag="ACG80L_001719"
     CDS             complement(5060..6043)
                     /locus_tag="ACG80L_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS5023948.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6458..7228)
                     /locus_tag="ACG80L_001720"
     CDS             complement(6458..7228)
                     /locus_tag="ACG80L_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023949.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7221..8156)
                     /locus_tag="ACG80L_001721"
     CDS             complement(7221..8156)
                     /locus_tag="ACG80L_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023950.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8472..10121)
                     /locus_tag="ACG80L_001722"
     CDS             complement(8472..10121)
                     /locus_tag="ACG80L_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5023951.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10238..12841)
                     /locus_tag="ACG80L_001723"
     CDS             complement(10238..12841)
                     /locus_tag="ACG80L_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5023952.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12853..13902)
                     /locus_tag="ACG80L_001724"
     CDS             complement(12853..13902)
                     /locus_tag="ACG80L_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5023953.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13928..14242)
                     /locus_tag="ACG80L_001725"
     CDS             complement(13928..14242)
                     /locus_tag="ACG80L_001725"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS5023954.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14258..>14692)
                     /locus_tag="ACG80L_001726"
     CDS             complement(14258..>14692)
                     /locus_tag="ACG80L_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5023955.1"
                     /translation="KININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQKDVF
                     DRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKD
                     TDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4345 a         3048 c         2484 g         4815 t
ORIGIN      
        1 acagcttcaa aaacaaagtc ttgatataaa tgtctcgata ccgaatactc gaaaggttta
       61 ccagacgata gagcaacaac tttgtctatc tcaatcataa attcacctgg atctattttt
      121 aatatttttg cgtcaaattc attcgcagta tctcctttga tacgtatgtg cgaagtcttt
      181 atttccagac ccagttcatt tgtgatataa gcatatacag atttctttaa atgttttggt
      241 tccaagcctg gtatgactgc taatggcatg aatgtttgct caatcgagta tggttgacaa
      301 tcaataaaac ggcttcgtac aatattatat acatagtctt ttacactgat acttaattta
      361 tcagctactt ttttcgatgg aagttcaatt gagaaagtat ggatttctga atctacgtgt
      421 tcttcaccaa ccacgctaaa caaaccttcg tttggaccaa gaactcgtga gttatcaatt
      481 ggtgttctga cgacttcagt cccataccca cttctacttc gcaaaactcc ttcggaaaca
      541 agtaagtcta cgcctttctt tactgttaac ttgcttgtat gataaataac tgccaattca
      601 tctcctgtag gaagtttctc accaatttta tactctccat ttacaatttt tttctttata
      661 tctaagtaaa tttccatata cttcgcaatt tttttcaatg gtgaccctcc aatcaaaaac
      721 atatcttcat ctatacttta acataaataa accgataaaa tccacatcaa aattatacac
      781 acaataaaaa agatatatat atttaaacaa aactatttac tttaagttgt aagcggtgtt
      841 atcattaagt ggaaacagga ggtaaaaaaa ttatgacaac tatgaaaaag ctaacttttc
      901 cagatgattt ttggtggggt tccgcttggt cagctgagca agcagaagga aaaggagaaa
      961 caggaaaagc agagactatc tgggatcgct ggtttaaaga acaaccttac cggttttatc
     1021 aacgaatcga ttcctctatt acaaccgatc acatccatca atataaagaa gatatagaac
     1081 taatgaaaaa aactgggcat aactcctttc gcgtttctat ttcttgggca cgaatgtttc
     1141 caaatggcgg taccggaaaa gtaaatccca aagccatttc tttctatcga aaattatttt
     1201 ccgaaatgaa taataacgga atcaaagtat ttgccaactt atatcatttt gacatgcctg
     1261 ctactcttca agataaagga ggatgggaat cacgtgaagt agttgatgct tatgttcatt
     1321 acgctgaaac ttgttttaaa gaatttggtg acttagtttg tcattggttt accttcaatg
     1381 aaccacttgg gcctatttta ggtacttatc ttgaagattt tcattatcca aacctcattg
     1441 actttaaacg cggagcgcaa gcagcattct tcactatact tgcacacgca aaagcaatac
     1501 aagtatttaa agaactagct cttcctagta aaataggcgt tattctaaat ttgagtccta
     1561 cttttcctcg tagtcaaaat cctgctgatg tgaaagcagc agaaatcgct gatttatttt
     1621 atacgcggag ctttttagat cctatggtta aagggaattt ttctaaaaag cttatcctgt
     1681 tattacaaga atataatcag cttcctgaag attatacgaa agaagatctt gtatgtatct
     1741 acgaaaatac cgctcaaatt cttggactaa actattacga gccacgtcgc gtcaaatctc
     1801 gttcttctat ggtggataca tctagtccat ttttaccaga gtggtttttc gaaccacata
     1861 tcatgcctgg acgacgaatg aacgaatacc ggggatggga aatttacgaa aaaggaattt
     1921 atgatttgtg catggatatc aagaaccatt atggaaacat cgaagcattt atctcggaga
     1981 acggaatggg cgttgcggat gaagaacgtt tcatggatga tacagggcaa ataattgatg
     2041 attatcggat tgagtatatc aaagatcatc ttgcttactt atggaaggca ataaatgaag
     2101 gatgtaacat taaggggtat catctttgga catttattga ttgctggtca tggatcaacg
     2161 cctataaaaa ccgatatggg ctggtttcac tcgaccttac tactcaaaaa agaacaatta
     2221 aaaaaagtgg cgaattttac aagcaattaa gtgatacaaa tggatttaga tacgatgtag
     2281 acttgttagt tgaataatat tctaaaattc cacttgaagt gaacacatta gaaaacaaga
     2341 ataatgagaa ataccaataa caaatacttt ttcttcatac ttcatttata cttgtcagta
     2401 taagagactg tgacaaatgt agtgatcctc agaagttagg tctggaaatc taacttgaga
     2461 agattttttt aaactacgtc cagtcttttt tatttgttta aaattgaagt tgacaagtct
     2521 tcattaaaaa tttagaatat tcacaagttt gacgacaatt atgaaaattt atgtcgtgct
     2581 aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa agacacgtta
     2641 cctaccgttt ttggttatat cggtattgga cttgcatttg gtatcgttgg gaaagctgcc
     2701 gggtttcatc cgctggtcgt cacgttgatg tccctgctga tctatgctgg ttctgcccaa
     2761 tttatcacgg tcagcatgct tgctagccat agtccactgc tctccatcgt tctctcgacc
     2821 tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta tttcaagaaa
     2881 aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag tttcgcactg
     2941 ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat gaatacagct
     3001 aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact tttagggaat
     3061 tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat gtttatcggg
     3121 ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca gttgatcctg
     3181 attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag taacttagtg
     3241 attttagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca ggccttcttt
     3301 tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt ctaggatcct
     3361 accatttgtc ttactgaaaa aattcgattt gccgcaaccg ttgcttgagt atttaagttt
     3421 tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc aaaatatcgg
     3481 acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac ttttagctgc
     3541 tattctttca aaaagcttat tggtgatcgt cttggtagga attctttccc tttcgttgat
     3601 tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg cgctcttgtt
     3661 tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt gaacggacca
     3721 ctaacaacga cttttcctaa tatatgtcct gtttctacgt ctttggttgc ttgtttcaag
     3781 ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat aatcccttca
     3841 tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc atagttatag
     3901 tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt ccaaacacct
     3961 atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg tcggacaagg
     4021 tgtttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata ctctacttga
     4081 tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat tggatagtct
     4141 acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta gacttctagg
     4201 ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccagctcc gttgatcact
     4261 aagatttttt tcccgcggtt ggcattttct tctggagtca gaccaaattt ttcaaataac
     4321 aattcataag cggtcaatga ggtcaacggc aaggctgcgg cttcttcatc tgaaagattt
     4381 tttggtgcaa gagcgacaat acgttcgtca accagttggt attcttgatt gcttcctgct
     4441 cttgtcgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg cacttcatca
     4501 cctacagcaa caactttccc gactccatca aaccctaaga tacgtaaagc atttcttatc
     4561 ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt gacttttacc
     4621 agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa gctttttgga
     4681 tcatcgatcg acaacccttc ataaaaaccg actaccttca ttaattcctt ttctgacata
     4741 cgaattcctc cttagcttca tcaagttgga gcatccatac agttttcatt tcaatatata
     4801 tagtatgcta tttgttccac ttttattcat cataattatt ctcttactta aagtaaacta
     4861 tttgcacttt tttggttaaa aaagaggttg cgacaagact tttgtcacaa cctcttttaa
     4921 acggtgttca atcaactgac cctcggtatt cgctcaaaaa acgacaaaat atgaggagct
     4981 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacga cctctaattc
     5041 acggtgttca agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg
     5101 tgtctaagat ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt
     5161 catttgctcc tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg
     5221 aacgcgggat gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga
     5281 tcagttcttt ttctgtatgt tccctcggtt ctttttcaaa tgcataagga ttcttgaaaa
     5341 tcccccggcc aatcatgatg ccatctacgc catatttttc ggccaattct tctcctgtct
     5401 ggcgatctgg gatatctccg ttgatcgtga tcagtgtttg cggagcgatt tggtcacgta
     5461 aagcaatgat tttagggatg acttcccaat gtgcatctac tttactcatc tctttacgcg
     5521 tacgcaaatg gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt
     5581 ccatctcaga catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag
     5641 ctttagccgc ttcaatcaat tgtgccgcta cttctgggcg aaggatcaag ccactgcctt
     5701 ttccacgttc tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc
     5761 ccatttctgc catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat
     5821 gggcaaccat cggctgttca tcttctgtaa aggtcaatct tcctcgtaca ctgtcttttc
     5881 catctggatg acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg
     5941 ctgcatgctt tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa
     6001 caaaaaaagg ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca
     6061 ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca
     6121 aaaaaagaag caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaagactgtg
     6181 acaaaaatct tatcacaatt ccttttccgg aataaatggt gttcaaaagc ttgcatctgc
     6241 ggtaataagt ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
     6301 acttgatgca gaacagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc
     6361 ctcggtatta gctcaacaaa cgacaaaata tgaggaacta ttttcccgtt tgtttctcta
     6421 atactcaggt caaaacgctg atttcacaac ctcttttcta acttctcaat cctcttcctt
     6481 tattgatcaa ataccaagta acggcgtaca aaacaacgat gaatagcaca agtacgatca
     6541 tcgatgttgc tactggcaca tcgattgttc caaggaatcc gtaacggaaa ccggagatca
     6601 tataaacgat cggattgact ttcgaaacag cttgccaaaa tggcggcaac atagagatgg
     6661 catagaaaac gccacctaga taagttaacg gctgcaagac aaatgtaggc acgatcgata
     6721 catcgtcata agactgagca aaaatcccgt tgagcagtcc tgctaacgaa aacaagattg
     6781 ccgtcatcaa taatgtaatg atgaccatga accatgaata cacatgaagt ggtacaaaga
     6841 acaaggaaat gatcgttacg agagagccga cgagaatact gcgacccact ccgccaatca
     6901 caaagcccca aatgatcaca tgcgtaggaa caggcgctac caagatttct tcgatgtttt
     6961 tctggaattt ttgagagaag aaggacgaag aaacgttggc atacgaacta gtgatagcag
     7021 acatcataat caaaccaggc acgatgaatt ccatatatga aaaaccaccc atgtcaccga
     7081 tccgaccacc gatcattttt ccgaaaataa caaagtacaa ggatgtcgtg atcacaggcg
     7141 gaaccagtgt ttggacccag atacgcaaat agcggttcgt ttccttgata gctaaacttt
     7201 ttaatgcagt aaaataaaga ttaaacattt ttctcctccg tatgattatt ttcttctgtg
     7261 attttcaaaa acagttcttc taaacgattc gacttattcc tcatagaaag aactttgatt
     7321 ccttgtcttg tcagctgatc gaagattccg ttcacccctt gatttcgttc aacttcgaca
     7381 gcgattgtct gttcatcttc aaatgtacaa gcgtatcctt caatcacagg tttggtctca
     7441 tatggcgcta aatcaaagat aaatgtttca aattgcagtt tggatagaag ctctttcata
     7501 ctagtattct cgatcagttc acctgattgg atgatcccga tgttgcgaca aagcatttca
     7561 gcttcttcta aataatgcgt cgtcaaaatg atcgttgtac cattttcgtt caattctcgg
     7621 agaaactccc acatctcgcg tctcaattca atatcgactc ctgctgttgg ttcatccaag
     7681 atcagcaaac gtggttcatg catcaatgcc ctagcaatca tcagtcttcg cttcattccc
     7741 ccggaaagca tacgtgcacg gacatttctc ttttcccaaa gattggactg cttcaaatat
     7801 ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt agtagcctgc ttgattcacg
     7861 acgatttgct ggaccgtttc aaagggattg aaattgaact cttgcgggac taatccaatc
     7921 tgttgctttg ctcggactag atccgtatcc agatcataac cgaagacatt cacttttcca
     7981 gatgttttat tgactaaaga agtaatgatt ccgatcgttg tagacttccc agctccattt
     8041 ggtcctaata gcgcatagaa atctccttct ttcacggaaa gatcgattcc tcgcaatgct
     8101 tcgaccccac ttccataaac ctttttcaaa ttatttattt caagcgcatt tgccatttat
     8161 ttcctatctc cttatagaca tgattcttta ttgtatcatg gaatacagca gatgaaaaaa
     8221 caacttggtt ccagcgaaaa atcaggttgc tgggctcagg aaaatctcct aatgaaagac
     8281 atcaatcgga cgaagtcaga caaccttttc agaaaaaaca aatcgaagat aaaggattac
     8341 tattcatatg tgcaacaaaa ggagcgtgag acaaaagtaa agaacacttt tgtctcacgc
     8401 tctaaacacg tataaacggc ggaagcaaaa gccaagattt ttgatccaat aaatactttc
     8461 gatcacttcg cctatcccaa tacatgccac tgccgctttt catcaaataa cgcggcttcc
     8521 gtttttgcgg tttctactaa agtctctggg taatccaaaa ctttcagcaa ggcaatcgca
     8581 tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa aactgattcc ttttccttct
     8641 gttacttgtt cttcaaagtg gacattctca caataattct ttaaaatctc tgtcaattcg
     8701 atatcatgtg tggcgacaaa tgctaaagat ggatactccg caagccaccg aacgactgaa
     8761 gcggaagaag caatccgttc gatcgtattt gtccctttta aaatctcatc tacgaagcag
     8821 tagcagcgtt ctttggaagc cacttggtcc aacaaacgtt tgatcgattt gatttctgaa
     8881 acaaaataac tgtcgccttc aaataaatca tcttcgattg ccatggaagt caacacatgt
     8941 ccaggttgca gggtaaactg ttctgcaatc gctgtttgga tcgtctgtgc tagaatacag
     9001 ctgatggcta tacttttgac atacgtagat tttcctgatg cattggaacc cgtgactaat
     9061 gtattcttct gccagttgac cggattcact accgcttctt ttaacaatgg atgatagata
     9121 tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc aagtgatggg catataagta
     9181 cggtaattca atattgcagc ggctacttct aattctccca gcaattccca cagcttgatg
     9241 gcttcagttg aatgcttttc taatttagtg agaacgaaat gataggagat aaatggcagc
     9301 atgaacatga tgttgacata ttcaaaaatc aactctgctt cactcccact cttcatacga
     9361 aatgaaaaac ctaatttagg gatgtctctt aatggtttag tttgctgttt cagctcttct
     9421 tgaagtggtg tcggcagttt tgctagctga ttggcggctg aaatcgttga aaccaaataa
     9481 ctcatgctat tcagctctgt ttctagtgtt tgtttcttca ctagataata gaccgtattg
     9541 aatactaacc caccgattgc caaatagatt cctagtactt gtcctaaaag aagcaaaccc
     9601 aaacccacca gcggaagcaa gccaagaaaa ataaataaac ctacgcgtcc aatcttttta
     9661 ctttttccat ccgctagata ttgttttgca aaattatgat ctttttttcc taaacgagcg
     9721 aattgatatt ggattttttc tctcagttga ggattttcct gataaaaagc aatcagtttt
     9781 tctaactgct gatcttcacc aaaatcaaag ctgcgtaaac gttgatacaa cgcttctgaa
     9841 cctacactag aataggtact gttgattcct tcgaatactt cgaacatatc caggtcatac
     9901 caagtaagat catcgatttc gctatcccat gaacggaact tcttctccgt gagccatgct
     9961 tccttcaagc tttcttcttt atcaaatctc ggttgataag gaattttttc ccatttgcta
    10021 cgaactgttt cctttaattt caaacgtgaa aaaatgtcta aagcaactaa taaaataaca
    10081 gctgcaataa taattaaaac gataaattct ctttgaccca tatccctctc cgttccttta
    10141 tttgttagct ttagtatagc actgttgcgc tatttgaaaa gagggtaaaa aaacaccttt
    10201 caaaataaaa ggtgtttttt tcttgtatat tacatattta tctctcattt atcaaaattg
    10261 acaaataatc atagggagga attgttgtta attcttgttt attctccagt gcattgacaa
    10321 ctgtcccaag ggaaaggata gcctcaggta ctattttttc ttgttctgtt ggattagcta
    10381 agcgaagaac aaatccttgt tcatgaaaat aagaaggata aatcgatgat acaattaagt
    10441 cttcaggtaa ttcaatcaat gaaaatttac gaggaacatc aagttcattt tgtaatggcc
    10501 aaatcttgtt atctaacctg tgaataaata cattcagttt ctgcttttga taagaaatag
    10561 attgttctaa ccgtttttct gcctgttttg ccactttgaa ctcattaaat cttccttctt
    10621 ctacattaaa tccaaaagtg actttatact cacctatctc ttgagctagc ggtgtttcca
    10681 tcatgacatg cccttcattt gtcgtatctc ctgaagctcg cccaggtcgc caagcaagat
    10741 taggttttcc aagttcccct gttgttgaaa ataaggttat gattaattga ttttctatac
    10801 gttcatactc ttttaaacca tctgcataaa acgtaaaata atgatcattt tctgccacag
    10861 agacactctt atcgaatatt tctaaattaa caggtttctc taccatagtt tctgaccaat
    10921 tcggaggaat gacatcaggg gtattttcaa taaatccgtt ttgaatttgt gcaattgatt
    10981 tttcagattt atctaaaaca ttaattgcta agcgcatacg atgcgagtaa acagtattat
    11041 gaattttgag tgtgccttca acgatttgac tttctcccag actgaacgat atttcataag
    11101 atatttcttc catttcgggt ttttcagata aacgatcttt taaatcttta ggaagttgag
    11161 cttttccgta aacaactaat gtttcttgta aagaatcttt atatacttta gctgtttcta
    11221 attttagaga tagttctgta tctccttcta atggtgaata atcatacgta tcgccatcat
    11281 ttgctgaatc gatcaaatga acgaaatcaa gatattgtct atctccaact atcaaattca
    11341 attgcccatc tttaaaagaa agtttatatt tattgttact tatctcagtt ttgtctatct
    11401 cgattgctgt ttctaatttt tcattatttt tctcaaattc aacaacagtg tatcctagtg
    11461 ctggcacatt tacatttatt tttatgtcaa gttcataata agctggttca tcaaaatatt
    11521 cgaaacctgt agcaatcatc cgataagcat ttttccgttc tggaaaataa acttcttttt
    11581 caatgatatt atagggacta cctttgaagt gtattttctt tgaatcagct actacatgca
    11641 cgattttcga tccactataa ttctcagtcg ttgtgttgaa aacaaggact tcattcgatt
    11701 ttagctttaa tgcatcagcc atccgtttta caataaggtt ttcgattccg tcagctatct
    11761 cattagcttc tttgattcga tggaaaatat cttccgcaac attatctgaa acacagccac
    11821 caatactatc atgagcctgg ttttgcaaaa tctttttcca taaccgacga agtaggcctt
    11881 tactgatatg gataccattt ctttctgcaa tcagcatgag cggctcaact cgttttagaa
    11941 ttttttgttc caatttaaaa ttttctattt tcatttttgt tcttactgag ccaatagaac
    12001 gatgaacacg agagtaaacc ggctgacgaa gttcaccttt atattcaggt aattcttttt
    12061 tgtttctgat aacttctaca taagaaggat aatctgaaat aatattgtgg aatttactgt
    12121 atttattcaa cttctctacg acttgatcaa aatttaaaat cattgacttt tgatctacac
    12181 ccgaaggtac taaaatatct ttgttttttc cttgcatatg aagcatttca actgcttcat
    12241 ccaacttttc ttttgcaaat tcaggaatat ctttaattgc gtctaaggtc atcattccag
    12301 tcatataacc aaatggaaaa ttcatggcat aaacaaattt atctcccaat cctttccaga
    12361 taaaataagg agatggaacc atcgtttcaa agtttaatcc tcgccaacac atgaaattat
    12421 cgatttctac ttgatgcaaa agtgtaggca aattcgcatt gaacccaaac gtgtcaggca
    12481 aataccctac catcattgga tctccatatt taaggatcgt atcactgatt ccaatcatta
    12541 agtttcttaa aattgattca gcatccacta ataaggcatc tgtttgtgca taccacggtc
    12601 ccacgaatag ttgtcctgtt ttcatcagtc gttggataac cttaagcttt tgcggattga
    12661 tctccaaata ctcatcgacg attgaagttt gtccatctaa acaaaagttt actttagggt
    12721 tcctttccaa ttcatctaga acttcagtaa agacctgatc agacaatacg atggcatctt
    12781 catttgtaaa ataccattct ctgtcccagt gcgtgtgttg aattatatgt gcatttacca
    12841 tatcagattc tcctactttc cttcttctac tgaatttact tgttgttctt ttttatactt
    12901 gatggatttt agtgcattga ccattaacgc tgtaaccact cccccaataa caattgatac
    12961 taaatataaa ggtatattcg taacaaaagg aattgccata aaggttgaaa gcattgtttc
    13021 ttgagtaaca ccaaaaaatc catttagacc acatgctact gcagtgccca ccacgatgct
    13081 tggtattact cgtaaaggat ctttcacggc aaacgggatc gtcccttcag taatccccac
    13141 aaggcccata atcatagctg tctttgcatt atctcgttcc gattcatcat aaagtttttt
    13201 gtttaaaatt gttgctaaaa acattccaaa tggcggaata gcaattccta ataacatggc
    13261 tgtactaggt ccaaagcttc cgcttgaaaa agaagcaacc ccaaaagcat atgcgatttt
    13321 attgataggt cctcccatat caaacgccat cattcctcct aaaatagccg acatcaaagc
    13381 agcatttgct cctgacatgc cttctagcca attagtcagt cctgttgtca ttgcttgaat
    13441 aggcgtagct agtacatacc ataatatgat tccaacaact aatgtagtaa caaaaggaat
    13501 cagcataatt gattttagac taataacttg catcggtact tttacttttt tcaatgcatt
    13561 agctaaatag ccacaagcta atccagcaac cattcctcca ataaatccca taccaagatt
    13621 ttgacacatc atcccagtga taaaagctgg tgctagaccg ggacggtcag caattgaata
    13681 tgcgatatag gctgaaatca tcggtacgat aaactgtaat cccgtctgac caatcattcg
    13741 caggactccc cagaatgtat ccgtctggtc ccaaacacct gtgccaccaa aaatgactgc
    13801 aatagaagtt agtaaccctg ctgctacgac aagcgggatc atataggaaa ccccactttg
    13861 gaaatgattt agaatttctc ttccttgttt cttgaattcc attagtaact cccccacgat
    13921 atatttttta tttgttaatt aatgcaattg cttcatcaat cgtctcttca ccatctttta
    13981 ctacctctga ggtccctact gttaaaatcg gtttaccgtc aaagcgacct cgttctgaaa
    14041 catttgtatc tactgcaaat atgacaacat ctgcctcgga aacatccgaa attgaaagtt
    14101 tgttttctat ccccatagcc ccctgtgttt ctactttaat atctaaccct ttcttcttcg
    14161 caaacttttc taaattagct tttgccatat aagtatgggc aatccctgcg atacatgctg
    14221 ttactgctac tatttttgtc atatgaaatt cctcctttta aaatgataga gcttttacca
    14281 attctttttt atttctcgaa tcttttattc ttttaacaaa ctcgtcatcc atcaatcttc
    14341 cagataattc tgctaaaata cgtaaatgat tagtaccttt atcagtgtct tttattgcta
    14401 acaaaaagac gagatcaact ggttgatcat ctaatgcgcc ccattcaaca gaccgcttta
    14461 acttggctac agccactgtt gattcaatta ctgctaggct tttgccatga ggaatggcga
    14521 tatttttccc aattcctgtg gtcatatggt tttctctgtc aaatacgtct ttttgaaatt
    14581 gtttgtggtc agacacaaac ccattagtct ctaacaattt cgataatttt tcgactacac
    14641 tttttttatc agatgcattt aaatctatca ctatattatt tatattgatt tt
//
LOCUS       DAZUMB010000066        14388 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000066 DAZUMB010000000
VERSION     DAZUMB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.067"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            458..1426
                     /locus_tag="ACG80L_001727"
     CDS             458..1426
                     /locus_tag="ACG80L_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5023956.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            1444..2349
                     /locus_tag="ACG80L_001728"
     CDS             1444..2349
                     /locus_tag="ACG80L_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023957.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            2346..3152
                     /locus_tag="ACG80L_001729"
     CDS             2346..3152
                     /locus_tag="ACG80L_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5023958.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            3309..4277
                     /locus_tag="ACG80L_001730"
     CDS             3309..4277
                     /locus_tag="ACG80L_001730"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS5023959.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(4594..5118)
                     /locus_tag="ACG80L_001731"
     CDS             complement(4594..5118)
                     /locus_tag="ACG80L_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS5023960.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            5405..7216
                     /gene="scm"
                     /locus_tag="ACG80L_001732"
     CDS             5405..7216
                     /gene="scm"
                     /locus_tag="ACG80L_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5023961.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(7371..8357)
                     /locus_tag="ACG80L_001733"
     CDS             complement(7371..8357)
                     /locus_tag="ACG80L_001733"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5023962.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8380..8946)
                     /locus_tag="ACG80L_001734"
     CDS             complement(8380..8946)
                     /locus_tag="ACG80L_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS5023963.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(8960..9376)
                     /locus_tag="ACG80L_001735"
     CDS             complement(8960..9376)
                     /locus_tag="ACG80L_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS5023964.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(9833..11767)
                     /locus_tag="ACG80L_001736"
     CDS             complement(9833..11767)
                     /locus_tag="ACG80L_001736"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5023965.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            12069..13004
                     /gene="pplA"
                     /locus_tag="ACG80L_001737"
     CDS             12069..13004
                     /gene="pplA"
                     /locus_tag="ACG80L_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS5023966.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            13094..14197
                     /locus_tag="ACG80L_001738"
     CDS             13094..14197
                     /locus_tag="ACG80L_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS5023967.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            14210..>14388
                     /locus_tag="ACG80L_001739"
     CDS             14210..>14388
                     /locus_tag="ACG80L_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS5023968.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT         4545 a         2974 c         2784 g         4085 t
ORIGIN      
        1 agtcaacact tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt
       61 ttatgttttc aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt
      121 tagaaaattc attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa
      181 cacaactgaa taacttttac tttagctact ttagataggt ttgtattcat catgatggat
      241 acaaacgtta aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa
      301 agccacgtag acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag
      361 cattctactt tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat
      421 ggattttttg tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg
      481 attaggcgct gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga
      541 ggcatcagat ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga
      601 tgctgcctat aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa
      661 tctaacaatc gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga
      721 gaaactcgtc aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc
      781 tttattaaat gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca
      841 agcccattta gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat
      901 ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat
      961 cggcatcatg tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa
     1021 agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt
     1081 ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa
     1141 cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt
     1201 cgttgctggc gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta
     1261 cgaaagcttg ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc
     1321 aagtgaactt cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc
     1381 caaagcatta ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt
     1441 ctcatgacaa atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc
     1501 ctacttgcga ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct
     1561 gaaggaagct ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat
     1621 ccattgacag cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga
     1681 ctgcttcaca caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt
     1741 ttatattctg tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca
     1801 acgatctttc attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc
     1861 ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca
     1921 ggacttgcta ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac
     1981 acagataata tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc
     2041 tccttacttg cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta
     2101 attggacttg cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc
     2161 cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc
     2221 gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt
     2281 ctttctttcc cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt
     2341 gtcaaatgaa tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga
     2401 tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga
     2461 cgatcatcgg tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat
     2521 tttctgtcga tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg
     2581 tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc
     2641 gtttaacggt agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct
     2701 tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt
     2761 tggggttgga aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag
     2821 ctattacgtt attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca
     2881 ctgcagcgct tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca
     2941 ctgagcagaa tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg
     3001 ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa
     3061 aacagcagct cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat
     3121 taaaagacga tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa
     3181 cgcccaccct cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt
     3241 gtttcttcga aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc
     3301 ttgatgccat gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc
     3361 tttccggaga aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca
     3421 tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc
     3481 tgccctctga cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg
     3541 atttgaaagt acaaacgatg ttgacttctg aatctacgat gaaagatgcc aaactggcag
     3601 cagctcaagg agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg
     3661 gacgttttgg acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc
     3721 tccatccaga caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct
     3781 gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata
     3841 tctacatgga tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa
     3901 gcggcaggat cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc
     3961 attttccagc tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg
     4021 tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc
     4081 cagaaaaagc gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt
     4141 tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac
     4201 tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat
     4261 ctatccgtac gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc
     4321 ttttttctga attctcttca aaagactctc cttattctat cagctgacag tttctctatc
     4381 ctctagaaaa aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat
     4441 caatgcaaaa aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc
     4501 ctttttcaat cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact
     4561 tattttgccg taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct
     4621 cgcatagatc acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat
     4681 tcctcgtgtg actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc
     4741 cagccttctt aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc
     4801 ttctatttga ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc
     4861 gttatctgct atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga
     4921 agttcctgct aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca
     4981 agtcattgat ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat
     5041 agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag
     5101 catatctaaa taatccattt attttcctcc tttttattta tcctacttac tttttcctaa
     5161 ttctaggata cctacttata tgttactaca cacccttatt ttttacactt taacagtcac
     5221 catcatcaat agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag
     5281 aagcaacaat atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa
     5341 cagtttaaga ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg
     5401 aaaaatgaaa aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc
     5461 gtatggtgtg gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa
     5521 tgatctaact ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa
     5581 aaataagaca actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc
     5641 aaaaaataat tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat
     5701 acaaaaaact gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa
     5761 taaatttgtc gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa
     5821 tggatccagg cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga
     5881 atatattgat ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac
     5941 gatcactcca gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat
     6001 cgatttaact gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt
     6061 aacgatgaat acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt
     6121 catcaacagt tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa
     6181 tcatgaaaca gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac
     6241 tgatttgaca atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg
     6301 cattactcaa aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac
     6361 caatcgttgg gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac
     6421 aagcgacagt acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga
     6481 cagtacagat actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag
     6541 tacagatact acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac
     6601 agatactacc tctaccacaa gtgacagcac ggacacgtca gcttcaagcg acagtacaga
     6661 tactacctct accacaagtg atagcacgga cacatcagct tcaagtgaca gtacagatac
     6721 tacctctacc acaagtgata gcacggacac gtcagcttca agcgacagta cggatacatc
     6781 taccacaaca agtgatagca cggacacgtc agcttcaagt gacagtacag atactacctc
     6841 taccacaagt gatagctcag attcatctac cactacaagt gatagcacgg acacgtcagc
     6901 ttcaagtgac agtacagata catcctctac cacaagtgat agctcagatt catcaacttc
     6961 aagcgatagt acagacacgt cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt
     7021 ctcaaaaaca agttcggata acaggacgaa ctttaactta actaataaaa caagcaatga
     7081 caatggaaac aaatcaaaaa gagcacttcc taaaactgga agtcaatcga ataattggat
     7141 aacactcgca ggcgttatat tacttgtaat cggtttaaga atcactttta tgagttataa
     7201 gaaatcgaaa aggtaaaaaa atattcaaga taaaaaagag caacgtcgct aacggacgtt
     7261 gctctttatt tttgcttttt atcataaata caagaactac aattcaattt gtcaaaaaac
     7321 acgtagattt ccttctgaga aatttattta cgtatcgaac ctttccctat tcagtctttg
     7381 cgatccaaaa tcagttgggt aagggaaaag agattctctt ttgttcgaag agaggtggca
     7441 ggtaatttct ttatttcctt caaagccttt tctgtgtaag aggccgctaa cttcctagct
     7501 tcttccacgc ctcctgtatg ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc
     7561 attgccgctt tcttctctaa ataaggtagt agctttggtt tgtttttttg caaagagtag
     7621 atcaacggta aagaatagac cccttgacgc atgtcttcta gtacaggttt gccgatttcc
     7681 tggctctctt ttgtataatc taaaatatca tcgataatct gaaaggccat cccgatagaa
     7741 agtccgatat ctcctgcttt ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca
     7801 acagaacaac ttaattggaa cagttcagct gtcttacctg aaatattttc caaatattct
     7861 tttacagaaa gttcataatt atagcgatca tccatctgcc ctaattcgcc attcaatact
     7921 ttttccatac tgcgagaatt caattggaga cttttcaagg aagtggagta atcagacaat
     7981 agtttaaaac agcagacaaa taaatagtct cctgcataga cagaaacgac attaccaaac
     8041 tgactacgga tattaggctg accacgtcgc agatccgctt catcaacgat atcatcatgg
     8101 atcagtgtgg ccgtatggag catttcgata gaagctgcca aagcgatggc tttgtctcga
     8161 tcttgctctg gtccaaattg tgagaacaat agctgatagg caggacgcaa cagctttccg
     8221 ccggcatgga tcgtttcttt gatggcttgt tcgacaggtt tgtttttcag gcggacagtt
     8281 tcttccatcc gttttagtgt ttctgtcaat tctttactta aagcaggata gttttcccac
     8341 attggatgta gcttcataaa aaaactcctt gtgtgacgat tattttttgg taaacattgg
     8401 gctccatttt tgtttggttc cctgctcata atctttttgg aatcgttgaa gtgtctgcac
     8461 atgtcgtaaa agataattcg ctgcgatccc gattgcaatc cctgataaaa tccctaaaac
     8521 tgaaaggact ggcaagtaca acatgactgt ccatgattgt gcaatgaaag aagccatcaa
     8581 aagctgtccg acattatgca tgaaaccgcc ggccgcacta attccgatga tactgacacg
     8641 ttttggtcct aattgcttga ccaataacat tccaaaataa ctaagcaaag caccactcat
     8701 actataaagg aatgtggaaa tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca
     8761 aagtagtaag aagctgtctt gttttttcat ggtaaaaatg gcaacgatcg tgattagatt
     8821 ggctaagcct agttttgcac caggcgcaaa agaaaaagga taagggatca tattttccaa
     8881 taaaccaatg atcactcctt gtgcaacaag tagcgaaata taaatcattt tttgtaattt
     8941 actcatggtg tgtgaaggtc taataaatca gactgtcttc gtcacctcca tctgttgaac
     9001 ggacttcgat cactaattta tgtggcaaac agacgatggt ttcgccattt ttcgttgccc
     9061 agcctcgacg tacgcagatc tggtcatcgc aatctgcttc ttttatccgt actcgatcgc
     9121 catctatctc gatcaagtta taatctccgt gttcatcttt gtaagtatat gtgttgctct
     9181 cttctccagc catcaaaggg aatgttttga tttctgtgcc atccacacgc agtaccgctt
     9241 ctttttctgc tgttgcggtg ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa
     9301 gaacagtcaa aagcaaaata ataaaaacat cccaaggttt gagcagactt ttttcagcaa
     9361 attctttaag gctcattttt gttctccttt ttccagatta actcacccaa ttttagcata
     9421 aaaacaagag gttgtgacac ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa
     9481 cagctttcca tgttttctaa ttctttgggc ttattaccga gaagcagcag gtggaacacc
     9541 gtttataaca agaggttgtg acagaagtgg gcaacttcaa gaaataagaa ggaatttacg
     9601 aaaattactt ttcaagtttt tgtgaaattt cggcttattt ttcgaagaag ctacttctgg
     9661 aacaccgttt ataacaagag gttgtaacag aggttgtgac acctgtgtct tgctcatgga
     9721 ggcttatctc gggcatctta ttcctttaat tttacataaa aaggaggctg tgacggtttg
     9781 tctcagcccc tttctattga aatcttgcat tcaaataacg ttctttatct atttatttct
     9841 tgccgtactg ggaacttggt ctaccgtacc accaattacc tagtgtacgt tgtagccatg
     9901 gaattaccca gcgatccaac ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa
     9961 caaaaatgaa ccaaatgttt acccagtaaa acatgcctga taagcagaac gtgatcacca
    10021 atacaatcgt tgtcgcactg catagccatg tgaataatcc agcaatcaat gctaatgcca
    10081 aggcaatttc aaagaatacc atgaattttt ggaagaacat tgctacatca ttgtttggca
    10141 tgatcacttg catgatcttt gtgaaccagt ctggtgactt ctcaatcaca gccattggtt
    10201 cttcaccgta tgcataactc aaaccaaaat gtgcagcttg agtcgctgct tctccggcac
    10261 ttgcccctgc tccgctggca gctgtggttg catcaccagc agcagaagca ccactggcaa
    10321 catctgttac attgtattcc catgtccaag ggaacacact gtttccagtg aaccatgatc
    10381 cttcaccaaa ccaagtactt ggtttgaaca cttcaccatt cccaatgatt tttttggaag
    10441 cttcaacgaa ccaaactaaa ccatagaaaa tacgaagtgg cacactccat aagacattgc
    10501 cgtaacgaga agaatgtcct cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat
    10561 gcattaaata ttggaacata taataaccag aacgaatatc aaagaagtat ttcaaattca
    10621 cgatatgttt cattacgata gctaaaaatc cgcttaaatg gatcttatca aataggttag
    10681 ctacccccca tttggcacca atagaaacca taaatccttg atagtttcct ttaaatttat
    10741 gcttttctcc gcctttgatc gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt
    10801 cagcagcttg aacgatctgc ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat
    10861 caccaatgat atagatgttt ttatcttcgt agccttttgc ctgcatatat tcattggcga
    10921 tcaaccgctg tccgcgtgct gattctaatc caaagtcagc agcgtcagaa gttcctttga
    10981 caccagctgt ccaaatcagt gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat
    11041 gatcagcagc cacttctacg attggtgcat tcaataataa ttccacgttg tgcttacgca
    11101 aatagcgttc agcttttgca gcatcatttc gatccaacat attcaagatc gtcggcattg
    11161 cctctacgac catcagtgtg atttcgtttg gatctaattt ataatctttg gctaggcgat
    11221 ttttccagtc gatcagttcc ccaatcattt caataccggt aaatccagaa ccacagacaa
    11281 cgaaagtcag catggcttta cgcagttcat catctggttc caatgcagct tttgctactg
    11341 tcttctcaat atgttcccga atccttacag aatcttcaaa tgaccaaagg gtgaatccgt
    11401 gtgctttgac accaggtgta ccaaagtcat tcggctctcc gcccattcca aggatcagct
    11461 gatcaaaagg ataggaacct aatttggtct tgacgatttt tcgttcttta tcgataccga
    11521 ttacagtgtc tgtgacaagt tgtacattct tttgacgagc aaacaaatgc tgcagatcgt
    11581 attggattgc ttctggttct acacgtccgc cagctacttc atgcaactcg gtcatcatag
    11641 tatggtacga atgacgatcg atcagagtaa ttgctacatc ttcttttttc agtttctttg
    11701 ccaagtattt tgtagctgaa acaccggcat atcctgctcc tacaacgaca atattttgct
    11761 ttgccattga tcatccgctc cttatagact atctatataa tgaatatatg ataattcttt
    11821 tagtttgata cttattacac aatctccata tattatatag atgttctgtt cttttgtcta
    11881 actccttttg gatttttttc aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa
    11941 agaaaacgtt tgaaatatta catgggttat ttatcaaaaa tagttgactt ataggctaaa
    12001 aagattagaa tgtataatga atcacaaaga aaaattgtgg actagaaatg gaatggtggg
    12061 gtattaaaat gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca ttttcagctc
    12121 tagtgttagg cgcttgcggc gcagatgaca acggaagctc aaactcttct tctgaatcat
    12181 ctacagcaca atcttcgact gcaaaaagca caacagaatc atctgcaaaa gttgtagctg
    12241 gcggagattt gcaagacggc acatataaat tagaagaaaa aaattattca aatggttacc
    12301 gtgcagtctt tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg
    12361 tcaatgaaaa cggcgaatca aaaacaaaag atgcagaata caacaaaaac atggaagcaa
    12421 aatctggcac aaacccagaa aaatacattc ctgagttgaa tgaccaattc ttaaaagcac
    12481 aaagcgcaag cggtgtggaa gtcgtaactg gagctactca ctcatctgaa agcttccaaa
    12541 actacgcaca acaattgatc caagctgctc aagctggtaa cacagataca atcgaaatcg
    12601 acaatggtgc tgatttgaaa gacggcactt acaagcttga agaaaagaac tactctaacg
    12661 gctaccgtgt gcaattcgaa atgacagttg ctggcggcaa agtaactgaa tcaaactttg
    12721 attatatcga caaagacggt aaatcaaaac aagatgatac agaatataat gaaaatatga
    12781 aagcaaaaag cggtacagaa ccaaaaactt acatccctac tctgaacgac gaattcgtca
    12841 aagcaatggg cgaagaagat ggctcacctg ctaatgtaga agttgttaca ggtgctacac
    12901 acagctcaca ttcattcatc atgtacgcac aacaattagt gaatgcagct gaaaaaggcg
    12961 acactcaaac aatcgaagta gataatatcg taacagaaaa ataatcacat aatcatacta
    13021 ataaaagttg gagggtggga cgatagccag attaggcagt cgtctcacct ttcaaaatga
    13081 agagaggaaa aagatgcgga aaaaactact attttcctta atcacactgg gcatctcttt
    13141 tatggtgcta actggctgca gcaactcagc aaataaaaca accactgaaa gttcagcagc
    13201 taccaagata ttgaaagatc catattctga tgaacagttc ttgttaggga cttacgttcg
    13261 tatccgtgtg tatgatgaag gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa
    13321 agaacttggt gataaaatca cgatcaatca aaaaggttct gagatcgatg aagtgaatga
    13381 gcaagctggg atcaaaccag taaaagtttc tgatgacgtc tacacgttag tcaagcgggc
    13441 ctacgaatat agccaagatt ctcaaggcgg attcgatatg gcgattggtg caatcacaca
    13501 attatggcga atcggttttg atgacgccag aaaaccttcc caagaagaaa tcgatcaggc
    13561 attgaaatta gtggattatc ataagatcga actaaatgac aaagaaaaaa ctgtttatct
    13621 aaaagaaaaa ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca ttacggacga
    13681 agtagtgaaa gtcttgaaaa aacaaggagt gaccactgcc atcgttgact tgggcggtaa
    13741 tgtctatgtc ttaggccaca gcccaagagg cgaaaaccaa gactggacag tcggcatcca
    13801 agatccgaac atggctcgcg gatctgtttt aggttctatt aaagaacgga acaaaacatt
    13861 agttacctct ggaatttacg agcgttattt agaagtagat ggcaaaaaat accatcattt
    13921 gtttgatcca aaaacgggtt atccgttcga caatgatatc gcaagtgtca cgatcataac
    13981 agataaatca attgatggtg acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa
    14041 aggactggaa tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa
    14101 agacgataaa gtatatgtga ccgatccaat caaagacacc ttcaaattat cggaggactc
    14161 ccattacacg atgggtgacc gttctgaact aaaataagga gtacgaacca tgacactaaa
    14221 aatcttttta gaagtagttg aaatacgaac aaaggtggct agtgtatttc cttttgcggt
    14281 aggggtatta ttctctatcg ctttttttca tgagatacat tggctaaaca cttttttatt
    14341 ttttattgga atgctagttt ttgatttagc gactaccgct atcaataa
//
LOCUS       DAZUMB010000067        13884 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000067 DAZUMB010000000
VERSION     DAZUMB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.068"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACG80L_001740"
     CDS             complement(221..1423)
                     /locus_tag="ACG80L_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5023969.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACG80L_001741"
     CDS             complement(1426..1620)
                     /locus_tag="ACG80L_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5023970.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACG80L_001742"
     CDS             complement(1667..2515)
                     /locus_tag="ACG80L_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS5023971.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACG80L_001743"
     CDS             complement(2720..3070)
                     /locus_tag="ACG80L_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023972.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACG80L_001744"
     CDS             3248..4111
                     /locus_tag="ACG80L_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5023973.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACG80L_001745"
     CDS             4735..5727
                     /locus_tag="ACG80L_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5023974.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACG80L_001746"
     CDS             5743..6627
                     /locus_tag="ACG80L_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5023975.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACG80L_001747"
     CDS             6676..8283
                     /locus_tag="ACG80L_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5023976.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACG80L_001748"
     CDS             8284..9768
                     /locus_tag="ACG80L_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS5023977.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACG80L_001749"
     CDS             9945..10937
                     /locus_tag="ACG80L_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5023978.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACG80L_001750"
     CDS             10989..11858
                     /locus_tag="ACG80L_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5023979.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="ACG80L_001751"
     CDS             complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="ACG80L_001751"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5023980.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4249 a         2351 c         2870 g         4414 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag
    13861 tccgatatgc ggcgcgttct gtgc
//
LOCUS       DAZUMB010000068        13638 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000068 DAZUMB010000000
VERSION     DAZUMB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.069"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..307
                     /gene="yaaA"
                     /locus_tag="ACG80L_001752"
     CDS             65..307
                     /gene="yaaA"
                     /locus_tag="ACG80L_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS5023981.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            294..1418
                     /gene="recF"
                     /locus_tag="ACG80L_001753"
     CDS             294..1418
                     /gene="recF"
                     /locus_tag="ACG80L_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS5023982.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1415..3361
                     /gene="gyrB"
                     /locus_tag="ACG80L_001754"
     CDS             1415..3361
                     /gene="gyrB"
                     /locus_tag="ACG80L_001754"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS5023983.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3381..5852
                     /gene="gyrA"
                     /locus_tag="ACG80L_001755"
     CDS             3381..5852
                     /gene="gyrA"
                     /locus_tag="ACG80L_001755"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS5023984.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6088..6387
                     /gene="rpsF"
                     /locus_tag="ACG80L_001756"
     CDS             6088..6387
                     /gene="rpsF"
                     /locus_tag="ACG80L_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS5023985.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6437..6949
                     /gene="ssb"
                     /locus_tag="ACG80L_001757"
     CDS             6437..6949
                     /gene="ssb"
                     /locus_tag="ACG80L_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5023986.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6975..7211
                     /gene="rpsR"
                     /locus_tag="ACG80L_001758"
     CDS             6975..7211
                     /gene="rpsR"
                     /locus_tag="ACG80L_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS5023987.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7354..9327
                     /locus_tag="ACG80L_001759"
     CDS             7354..9327
                     /locus_tag="ACG80L_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS5023988.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9333..9785
                     /gene="rplI"
                     /locus_tag="ACG80L_001760"
     CDS             9333..9785
                     /gene="rplI"
                     /locus_tag="ACG80L_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS5023989.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10059..11426
                     /gene="dnaB"
                     /locus_tag="ACG80L_001761"
     CDS             10059..11426
                     /gene="dnaB"
                     /locus_tag="ACG80L_001761"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS5023990.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12206..13498
                     /locus_tag="ACG80L_001762"
     CDS             12206..13498
                     /locus_tag="ACG80L_001762"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS5023991.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4545 a         2467 c         3001 g         3625 t
ORIGIN      
        1 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
       61 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
      121 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
      181 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
      241 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
      301 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
      361 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
      421 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
      481 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
      541 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa
      601 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
      661 tagtaaaagg cagcccgcag accaggcgga agtttttaga tatggaatta ggccagatca
      721 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
      781 acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
      841 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
      901 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
      961 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
     1021 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
     1081 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
     1141 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
     1201 ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
     1261 atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
     1321 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
     1381 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
     1441 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
     1501 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
     1561 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
     1621 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
     1681 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
     1741 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
     1801 tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
     1861 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
     1921 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
     1981 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
     2041 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
     2101 cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
     2161 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
     2221 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg
     2281 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
     2341 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
     2401 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
     2461 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
     2521 attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc
     2581 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
     2641 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
     2701 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
     2761 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
     2821 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
     2881 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
     2941 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
     3001 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
     3061 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
     3121 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
     3181 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
     3241 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
     3301 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
     3361 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
     3421 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
     3481 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
     3541 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
     3601 tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg
     3661 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
     3721 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
     3781 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
     3841 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
     3901 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
     3961 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
     4021 ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt
     4081 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
     4141 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
     4201 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
     4261 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
     4321 ctgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
     4381 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact
     4441 atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag
     4501 aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
     4561 ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt
     4621 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
     4681 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
     4741 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
     4801 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
     4861 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
     4921 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
     4981 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
     5041 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
     5101 ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt
     5161 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
     5221 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
     5281 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
     5341 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
     5401 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc
     5461 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag
     5521 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
     5581 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
     5641 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
     5701 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
     5761 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
     5821 aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca
     5881 agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
     5941 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc
     6001 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
     6061 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
     6121 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
     6181 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
     6241 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
     6301 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
     6361 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
     6421 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
     6481 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
     6541 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
     6601 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
     6661 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
     6721 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
     6781 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
     6841 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
     6901 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
     6961 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
     7021 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
     7081 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
     7141 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
     7201 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
     7261 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
     7321 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
     7381 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
     7441 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
     7501 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
     7561 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
     7621 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
     7681 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
     7741 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
     7801 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
     7861 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattggat
     7921 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
     7981 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
     8041 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
     8101 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
     8161 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
     8221 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
     8281 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
     8341 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
     8401 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
     8461 atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
     8521 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
     8581 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
     8641 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
     8701 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
     8761 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
     8821 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
     8881 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
     8941 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
     9001 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
     9061 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
     9121 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
     9181 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
     9241 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
     9301 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
     9361 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
     9421 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
     9481 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
     9541 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
     9601 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
     9661 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
     9721 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
     9781 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
     9841 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
     9901 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
     9961 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    10021 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    10081 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    10141 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    10201 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    10261 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    10321 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    10381 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    10441 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    10501 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    10561 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    10621 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    10681 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    10741 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    10801 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    10861 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    10921 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    10981 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    11041 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    11101 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    11161 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    11221 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    11281 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    11341 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    11401 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    11461 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    11521 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    11581 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    11641 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    11701 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    11761 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    11821 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    11881 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    11941 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    12001 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    12061 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    12121 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    12181 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    12241 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    12301 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    12361 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    12421 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    12481 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    12541 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    12601 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    12661 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    12721 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    12781 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    12841 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    12901 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    12961 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    13021 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    13081 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    13141 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    13201 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    13261 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    13321 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    13381 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    13441 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    13501 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    13561 cgataataat aacttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc
    13621 acagtccctt tttccgaa
//
LOCUS       DAZUMB010000069        13398 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000069 DAZUMB010000000
VERSION     DAZUMB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.070"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..712)
                     /locus_tag="ACG80L_001763"
                     /pseudo
     CDS             complement(14..712)
                     /locus_tag="ACG80L_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            791..967
                     /locus_tag="ACG80L_001764"
                     /pseudo
     CDS             791..967
                     /locus_tag="ACG80L_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="ACG80L_001765"
     CDS             complement(1143..1712)
                     /locus_tag="ACG80L_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5023992.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ACG80L_001766"
     CDS             complement(1786..2109)
                     /locus_tag="ACG80L_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023993.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ACG80L_001767"
     CDS             complement(2263..3075)
                     /locus_tag="ACG80L_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023994.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3402..4277
                     /locus_tag="ACG80L_001768"
     CDS             3402..4277
                     /locus_tag="ACG80L_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFS5023995.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4291..4614
                     /locus_tag="ACG80L_001769"
     CDS             4291..4614
                     /locus_tag="ACG80L_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5023996.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4611..5363
                     /locus_tag="ACG80L_001770"
     CDS             4611..5363
                     /locus_tag="ACG80L_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS5023997.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ACG80L_001771"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ACG80L_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ACG80L_001772"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ACG80L_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS5023998.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="ACG80L_001773"
     CDS             complement(9827..12013)
                     /locus_tag="ACG80L_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5023999.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ACG80L_001774"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ACG80L_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS5024000.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="ACG80L_001775"
     CDS             complement(12235..12675)
                     /locus_tag="ACG80L_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS5024001.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13231)
                     /locus_tag="ACG80L_001776"
     CDS             complement(12749..13231)
                     /locus_tag="ACG80L_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFS5024002.1"
                     /translation="MKEITDFPLLPLDLLWHTTDYSIQAKKWFLQEQQLFMVSNPSWI
                     VEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKL
                     NREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN
                     "
     gene            complement(13113..>13398)
                     /locus_tag="ACG80L_001777"
     CDS             complement(13113..>13398)
                     /locus_tag="ACG80L_001777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024003.1"
                     /translation="YSKQGTLTRLLSQSLVFLRRYKWYDRTKFQREVREKNEDHRDWT
                     KWVGKIDPRKEDQRNHRLSATAARPFMAYNRLQYSGKKVVFARTAIVYGI"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DAZUMB010000070        13349 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000070 DAZUMB010000000
VERSION     DAZUMB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.071"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..167
                     /locus_tag="ACG80L_001778"
     CDS             <1..167
                     /locus_tag="ACG80L_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS5024004.1"
                     /translation="KKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENR
                     KKAMRKRVSR"
     gene            complement(229..2040)
                     /locus_tag="ACG80L_001779"
     CDS             complement(229..2040)
                     /locus_tag="ACG80L_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS5024005.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2037..2402)
                     /locus_tag="ACG80L_001780"
     CDS             complement(2037..2402)
                     /locus_tag="ACG80L_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS5024006.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2565..2960)
                     /locus_tag="ACG80L_001781"
     CDS             complement(2565..2960)
                     /locus_tag="ACG80L_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5024007.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2978..3940)
                     /locus_tag="ACG80L_001782"
     CDS             complement(2978..3940)
                     /locus_tag="ACG80L_001782"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS5024008.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3940..4152)
                     /locus_tag="ACG80L_001783"
     CDS             complement(3940..4152)
                     /locus_tag="ACG80L_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS5024009.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4173..4871)
                     /locus_tag="ACG80L_001784"
     CDS             complement(4173..4871)
                     /locus_tag="ACG80L_001784"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS5024010.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4891..5433)
                     /locus_tag="ACG80L_001785"
     CDS             complement(4891..5433)
                     /locus_tag="ACG80L_001785"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS5024011.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5565..6569)
                     /locus_tag="ACG80L_001786"
     CDS             complement(5565..6569)
                     /locus_tag="ACG80L_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5024012.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6566..7555)
                     /locus_tag="ACG80L_001787"
     CDS             complement(6566..7555)
                     /locus_tag="ACG80L_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5024013.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7552..8358)
                     /locus_tag="ACG80L_001788"
     CDS             complement(7552..8358)
                     /locus_tag="ACG80L_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024014.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8524..9480
                     /locus_tag="ACG80L_001789"
     CDS             8524..9480
                     /locus_tag="ACG80L_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5024015.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9557..10075)
                     /locus_tag="ACG80L_001790"
     CDS             complement(9557..10075)
                     /locus_tag="ACG80L_001790"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5024016.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10163..10312)
                     /gene="rpmG"
                     /locus_tag="ACG80L_001791"
     CDS             complement(10163..10312)
                     /gene="rpmG"
                     /locus_tag="ACG80L_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5024017.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10540..10986
                     /locus_tag="ACG80L_001792"
     CDS             10540..10986
                     /locus_tag="ACG80L_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS5024018.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11181..13076)
                     /gene="asnB"
                     /locus_tag="ACG80L_001793"
     CDS             complement(11181..13076)
                     /gene="asnB"
                     /locus_tag="ACG80L_001793"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS5024019.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4156 a         2831 c         2277 g         4085 t
ORIGIN      
        1 tgaaaaaatt tgtatctgga atattagtag gaagtctggc aacagccgct gccgttgctg
       61 gtttggttgc ttctgtgaaa aaaacagtga tcgatccaat tgacgaaaaa gaagcaatga
      121 tcgaagaaaa ccgtaaaaaa gcaatgcgta aacgcgtttc tcgttaataa ttatttaaaa
      181 atacgcatga agactttcta aaaaacagat aagtcatcat gcgtattttt atttgtcagt
      241 caatataagt tgtgctattt tatctgcctg tctcagattc catggatctg tataaggtct
      301 agctactgct gcagttagcc aatccacacc ttcaacaggt attccatagc aataaaagtc
      361 agactggatt tcccctgaag catcaatcac gcgattcgta cctcgctcaa catctatagc
      421 ttctgaatga taacttcctg tattatctga aaatgaaaaa gggcgaacaa gtttgttttc
      481 cttcaaattt tttatgacag gattacttga acgagaaaag cttgtttgtg gcaatcgagc
      541 ttcaatcaaa tagcgactgc tgatttcttt atcttgtaga gaaataacat agcggttgtg
      601 cttttgctga atatcaaatg gcggttcgat caaatgaaaa atacccgcat ctatcagtgc
      661 tgctaattct ttcgtccgaa tgattggcgg accgatcgtt aaaaagctat ttagatgagt
      721 aaaccatccc catagtattt ctttgtattc cttagcagaa aaaacgtgcc attcaatagc
      781 ctgccggatt ggatcacgcc agtcttttaa tgcatcaaat gttgaagcaa atgctcctgt
      841 gcaattgcct ttttccgctt cttttatctg cccttcaata aatattcggc tagcttctga
      901 aaagggctca ttctgatagg caagaggagt ttctaaatac gaccaatccc atctataagg
      961 gattagtgct ggatgctttc cgagacactc ctctgaagag agggagagaa aatcatgctc
     1021 aaaatttttc tttggaatcg gcaagtgata ttcttcgatg attttcttgt aataaaacaa
     1081 ctcaacgtct tttttcagat agtcaaaaaa tgtttcgcca gtcaaaaatc ccgactgatg
     1141 ccattgctcc aggttttctt ttgttagtaa ccgtggccat gccattgctc cgccttgctt
     1201 ttgatttctt cctcgagggt aataagggag cccccttctt gatccactgt atacaatcgg
     1261 ttccttgcct gatggatgat agaccagttt tcccttttcg ttctcgaagc gccctcctct
     1321 tgatactgta aacaatccga cataatcaaa aaaacttaag cctagacctc ttaaaaagac
     1381 attttcttta ggcggtatct tcgaaagatc tacatctgca ggattagaag gaagctgata
     1441 cagtaaaaca tttttctttg cgtagtctac ccacactctt tcttcttctg tctcatcatt
     1501 ttcccagtga ccgcttgcta aaacaagctg atcgatagaa tagctttgct gatctgtctg
     1561 taaaagataa ccatcttttc ttttgcttac tttttttact aaagaataaa caaactgaat
     1621 agaagctgga aattcttctt gcaactttga gaaaaaccag cgttgataga gtccgtataa
     1681 acatcgagta gattgctgat ctttttccag acgattgatt tcttctataa ttagttgctt
     1741 atttttggtt accgtctgtt cgatataatc tgtagcaggt cctttactcc attgaaacaa
     1801 gttgggtccc tgtgttacca atcctccact ggacaatgtt tcatctgtaa aaagcgtcac
     1861 ttgttgagaa acagaattca ttaataactc cggtggctga ttcaaccgcc aaactcgacc
     1921 tccaggacca ttgggatcga aaagaaaaag ttcaagagaa tgtctgtcct gattattatg
     1981 gagaatcctt tctgctacac ttaatcctct aggtccggca ccaatgatcc ctattttcat
     2041 ttgcatgcct ccatttcgat aaattttttc aataaaacag catggttctg atgttcattt
     2101 tttgcaccat aaacaaaagt aatgactgga tgttctttca taagttgact gagtttaaaa
     2161 atagcttgct tcttttcttc ttcgaaaatc aattcttgct ggtatttctt tgcaaatgaa
     2221 gcaaatcgtt ctggtacatg gtcaaaagtt ttgcgtgctt cattactagg tgcaatttct
     2281 ttcaaccaca gatctaactg cgctttttct tttgaaattc ctcgtggcca gaatctatcc
     2341 acgagtattc tatagccatc ctctttttca gcatcaaggt aaacacgttt taatttgatc
     2401 atagttgttt ccttctttct accttttcgc ttagcttata tatgtagata atagcacaaa
     2461 ataaaaaaaa tgaacggcac aactatccat tgcgccgttc atgtgatctt ggtcaaaatc
     2521 taatgattga ttcggttttt aaagctgtca tacaatcatc tgttttatac ttttttactt
     2581 ttgatttttc cagaccatcc gtcatatcca cccttaagta aataaatctc ttgataacct
     2641 gctttacgta atttatttgc tgtacggatg ctcaaacttt tcttttgatc gtaaatatag
     2701 acaggctggt cttttcgaat agatcctaat gaatctttca aaacagtgta agggatgttt
     2761 cttgcaccta aaatatgccc agcatcaaac acatcttttt cgcgaacatc gattacttgt
     2821 gcttttctca ttccttctcg aaattcttct tctgtcaaag tcgttgctga acgtttggcc
     2881 attactcgta gatagacttc ccaaaggatg attgcaagca caatcaatag caaaatgata
     2941 ttaattaccc ataaaaccat gtaatgcctc cgttttttta agcaaatttc aaggctaatg
     3001 aggcagcacc aatgacacca gcttcattac caagctctgc taatttgatt tttgttgatt
     3061 ctcttacttg cgggaaagta aattctttaa aatatttctc tacacggcta cgtaaaaatt
     3121 ctcctgctgc cgatactcct ccacctaaca cgatacttga cggatttaat gtattcccca
     3181 agtttccgca agctaaacct aagtagaagc atacgcgatc aacaaccatc aatgcaaacg
     3241 gatcgtcttc ttgagccatt tcaaagatat ctttacttgt tacttcttct ccgttgtcta
     3301 gcattgcttt cagttttgaa tcccctgcgt attcttctgc caagaaacga cctaggcgta
     3361 ccacacctgt agcgctagat acagtttcta aacatccgcg tttaccgcaa gtacattcga
     3421 acccttcagg atccacagtg atatgtccga tttctcctcc agctccgctc acgccgtgaa
     3481 tcaaatttcc gccagcaacg atacctccgc caacacctgt tcctagagta acgaatacaa
     3541 cgtctggatc gttttctcca gcacctttcc atcgttcacc taaagcagca acattcgcgt
     3601 cattatcgat agcgaatttg attcctgttc ctgcctcgat cagttttttc acaaattgaa
     3661 cttctgtcca gtttaagtta tacgcaccaa taaccgttcc agcttcgctg tccacgcttc
     3721 ctggggtacc catgccgatt ccgataaagt cttcggcttt catattatat aaattcaaac
     3781 gatgattgat tgattcaatg atttcaggaa cgatatgctt accatcatcc aaaatattgg
     3841 tatcgatgct ccatttttgc tgaatctctc cttctggtgt taaaattgca aattttgctg
     3901 ttgttccacc taaatcaatc ccaataatct tcttgtccat tacaaatttc ctttctttcg
     3961 gctttcttca attcggtgct ctctttttaa tatgcgccac gctgtcatgt atgtatcatg
     4021 atcaatcaaa cggctatcat acagattacg caattcgata gtcattaatt caatgtcata
     4081 gatgcgtgca cccacataga taaagatgcc aaattttttg aacagctgct gtacatcata
     4141 taagctctcc atctttaagc acttccttca tattatacaa gtaaaccata tttttttaaa
     4201 cccagtaaaa tacatattac aaaaagaaat acaaaaacta gtgtagaaag aatcctttgg
     4261 tgtatcgaat agcttgtctg ttgtttcggt agacccaaaa cattccctaa cactaatccg
     4321 ccaattagcc caccgacatg tccccaaaca tctacagagg tatccagaag accaaaaaca
     4381 aatgtcaaca caataaataa aagaaaatgt ctgactagct gctggatcgc tgggttatgt
     4441 ttaaaatgaa accctaagat aaacaacgca ccgaacaaac cgaatatcga agtacttgca
     4501 ccaccagaca gaacattcgc ttcgttaaaa gcaaagcttg ctgtatttcc cattatcccg
     4561 ctgaacatat aaatcaagaa aaaacgccaa tggccataga ttgcctcgat ttgctgcccc
     4621 ataaaataca gtatcagtga gttcattacg aaatgcatca gtccaaaatg gatgaagatt
     4681 ggtgtcacta aacgccacca ttcattgaag tgaacaagat aaggaccata cattccggca
     4741 taataaggga tctcaagtcg cggaaataat tccatcatga ggaagaccgc tgtctgtatc
     4801 cctaaaaaag cataggtcca taacggtcgt cttaaccatg cacgcaattt tatttgctgt
     4861 tgataattca tgaaaacctc ccatttcatt ttagtcaata aacaattgtt ggatcttctg
     4921 atcaaagctc tccggttgcc attgttcatt caattgttca cgaaaaacaa ggctacatgt
     4981 ttttttatga aaattattta aataccggtc gtaataacca ccgccaaaac cgattcgata
     5041 accggctcta ttgtagataa tacctggtac aatgaggaga tcgatcatct ccggcaaata
     5101 gagcttctct gattgtggct caagaacacc aaaactcgaa cgtacaagaa cggactgttc
     5161 atccatttca taaaacgcca tctgacgttt cggcaaagtt ttaggtacaa ctattttctt
     5221 ttgttctttt tctgctcgtt ggataaccgg ctgtgtatcc agttcaaatt cattcgataa
     5281 agtcgtccct atcacttttg ctgacttcca gtactcgctg gcgaatagct gtttatagat
     5341 tcgctgttct ttttcctttt tcttgccact tttggccaga tttttcagct tttcgattcc
     5401 tttccggcgt aatcttactt tcaattggtc caaaaactca cccttccttc tctcataaaa
     5461 tggtatagga aaaaaagaaa aatagcaatc gaactttcaa ataaatgcat aaatagaata
     5521 aaaagaagac ctagaaatga ttggccttcc ttttttaatg aatttcaact gttcagcgcg
     5581 tgtttttgtc tgataaaaag tgcatacaga aaatatggcg caccaaaaag ggcaatcaaa
     5641 attcctgtag gtacttcccc acttggtaga atcactctag caaccatatc tgcactaagc
     5701 agaaaaatgg aaccgaacaa accgctcaga acaaaggtcc agttattttc tttacgtacg
     5761 actagcttcg cagcatgagg agctagtagt cctacaaaag ataaactacc tgtcaccgct
     5821 acactaccgc ttgccaaagc aacagctaac aacaaaagga ttttttgact tttagccacg
     5881 ttaaggccta atccttgcgc gcgttcttga ccaaatgata gcaatacaat cctctgtgca
     5941 taaagtcctc ctagcggaat taagataacc gcccatggaa gcaatgccca aacataaggc
     6001 cagctcgcac cccagattga tcccgctaac catgcattca caaatccata attttcctta
     6061 gataattgga tcgtgcatag caatgttaat gcagaaaggc cggcattaac ggcaataccg
     6121 gacaataaga ttcgattcat acccaaaaag ccatttcgat cataggccgc aagatagaca
     6181 cagcaagcag ctaaaacacc acctagaaag gctacaacta gcaatagaaa cggcagcgaa
     6241 tagtgcgaaa agaaacctaa ataaagcatc actaaaaatc ctgctccggc attaatgccc
     6301 aatatactag aatctgccaa ttcatttctt gttactcctt gcaagatata accagaaacg
     6361 ccaagacaag ctcctgctaa taaactgatc agtaatcgag gaactcgaaa ttctctaaca
     6421 atcagcgcca aagaagatga ttctttccct atgaacgtgg caaaaagctg atccaggtaa
     6481 ataaaaggcg taccaatcat taaactacct atagacagga tgacaagtac aaaaagcaag
     6541 aaatagaacg tccaatatat ttttttcacc gttgttccct ccttacttgt atcagtaaaa
     6601 acggaacgcc aatcaatgca atgactaatc caaaaggtgt ttcaaaaggg ggattgatca
     6661 tcctcgctgt aaaatccgcc aacataacca atccaccgcc aactaaaaaa gagacaggta
     6721 gaacataacg atagtccgtt ccaaccattc cgcgaacaac tggcggaacc aacagcccaa
     6781 taaatgaaac aggtccgact aacgaaacgg caatggctgc taacactgca actagacaga
     6841 aaacaatcaa ccgcgttcgt ttaggatgac ttccaagagc taatgcatgt gtttcactca
     6901 aatgaatcaa agatattgct tttcctaatg aaaaagaaac caaaacagca agtatatata
     6961 aaggaaacaa tactgctaat tgtttccatg tgatattggc agctccgcct acaaaccaaa
     7021 aagcaagatc ctgctgcaga ttaaagaatt gggtaagtgc ttggctgata gcagtaaaaa
     7081 aagaactaat agctacgcca gctaaaatca gctgtaaagg attcatgccc attttcgaaa
     7141 actgcaaaaa accaaatacg agcaaagtta cagcaaaaga acctgccaat gacattagaa
     7201 acacagaaaa atcagaagct ttaggccaaa aagcaaaact aagggccatt gccaacgaag
     7261 cacccgcgtt gattcctagt aagcctgaat ctgctaaagg atttcttgtc accccttgca
     7321 tcaatgcacc gctcaatgca aaacaactcc ctacaagaaa tgctcccgcc acacgaggca
     7381 aacgaatatt tcgtatgatt tggtgacttt gttttgatct atcaaaatca aacaacgcct
     7441 gccaaaaatc tgctttttcc acagtcgctg cgccatttga aagagcaaca aaaaaaataa
     7501 tcaaaagaac aaaacagacc aaaagcagga gtctattttt cttcatagtc ctcataattg
     7561 atcctcctgc tcttttggca acaaatcata ggttagaaaa actggtttat ttgttcttgg
     7621 aagcgtgaca atcgttgcat caatctcaaa tatttttttc atacactctt ttgtgatcac
     7681 ttcttcaggt gtaccttctg tgatcaactt accgtttttc attgctaata aataatctga
     7741 aaaacgtgaa gcatgattaa tgtcatgaat cgacatcaag atcgttgttt gtttcatttg
     7801 attgatttca gtcaataact gtaggatctc taattggtgt gccggatcta aaaatgttgt
     7861 cggttcatcc aagatcaaga tatctgtttc ctgagcaagc gccatagcga tccagacacg
     7921 ctgggtttgt ccaccagaca gcgttgcgat atcccgcata cgaaatccag ctagacctgt
     7981 agcatccaac gcccactcaa ttgcttcatg atcttgcgtg tccatgccac tgaacagttt
     8041 ttgataaggg tatcgtccgt aagatactac ttcttctact gtcatacctg tagggtgttc
     8101 actagtttgt gctaataatg caattttttt tgcaagttct tttgaactgt atgtagagat
     8161 atcagagtta ttgacaatga tctttcctga tttcacaggt agtatacggg taaaactttt
     8221 caataaagta gattttccac ttccatttgg cccaatcaaa ctagtgattt tcccttcagg
     8281 aatcgtaaca gacatgtttt caatgatttt tttgttttcg tagccggctg caatgtcagc
     8341 tgctcttaat ctcgtcattt cacccatcct ttcgctgaaa atcattctca attatgacgc
     8401 tgtttcaaca tttcgtcaag cgctactttc atatctatag ccaaatgaag cggttgacag
     8461 cgttcgaaat atcaggaata ataaccaatg agaaagattc tcaattatgt taggaggaaa
     8521 gaaatgaaat taaaaacaaa agtattcaca ttatctagca tattgctgct ggctggagta
     8581 ctgagcgctt gctccccctc ttcagataaa gaaacagcta caactgccaa ttcgacgact
     8641 tcatccaata gtactcatac agtcacagat acacttggac ataaagtaga gattccaaac
     8701 gcgcccaaac ggatcatcgg cagttactta gaagactatc tgattgcact aggggaaaaa
     8761 cctatcgccc agtggactgt tggcagtggt tccattcaag attatctgca agatgattta
     8821 aaggatgtcc caacgatttc ttatgacctt ccttatgaga aagtgttaag ctttgagcct
     8881 gatttgctgt tgatttcttc ttccgctact gtcgaaggcg ggaaatacga acaatatagc
     8941 aaaatcgcgc caacttatgt tgtaaaaaac ggaacagatg tcacatggga agaacaacta
     9001 aaagatgtcg gcaaagcttt ggcaaaagaa aaagaggcag aaaaaatcat ttccgattat
     9061 caaaaatcca tcaagaaaac tcgagaagaa ctagcagata aaatcgagaa caagacagct
     9121 gcagtgttgt gggtgaccaa caactcagcc tttatggtca gcaaaaatcg gtcaagtgga
     9181 agaatcgtat acgacgatct ggaatttggt gttcctgatc tagtggaaga ggtctccaaa
     9241 aaagccactt ctgactggtc tgctgtttcc tctgaaaagc tagctgaatt agatgctgac
     9301 tacatcatcc ttgtgaacag tgacaaagat gctgccatgt tcaatgaacc aagctggaag
     9361 aatctaaaag cagtgacaga caatcatgta ctgcaattcg gacctgaatc tagttggcta
     9421 tataacggac ctattgccag tcaggaaatg gtcaatgata tcaaagaaaa actaaattga
     9481 ttttaactaa aaaaagcatc ttcacgatta gtcgtgagat gctttttttc ttattaacaa
     9541 actagacgca tagattttat tcttcttttg aagcaaccct attttttact ctttcccatt
     9601 tttgaaaacg atgggcatat agattttctt gatccgttat cccctcatac tcctctataa
     9661 gggcgaagtc tgaaaaatca atattcccaa tatacgtatc gccttcaaac gtgctatcga
     9721 tcaatgttcg ccagatccga ccagtgtaag gaagaagttc ttgaaaaatc cgactaccac
     9781 ccgaaacata gatatcttct gagatcgttt gactatacga caaaagttca tcgatcgaat
     9841 gcattacttc cgcattttct ttcgtgcgaa aatttggttg cgttgtcaac acgatgatat
     9901 ggcgataagg aagtgaaaga tcccccattc cttcataggt ctttcttccc atcactaaaa
     9961 ttcgatccat tgtatgttcg cgaaagaaac gcatatcatt tggcaatcgc catggtaaca
    10021 aaccattttt gccgatcaga ccatttttat cttgtgccca catggaaata aacatattat
    10081 ccgctcctta tgattttaga tgttttgaga taaaaaaagt ctcaattctt tgaccagcaa
    10141 ggaattgaga actttaaaaa cattattttg tttcacggtg caaagtaact ttgcgttcgc
    10201 gtgggcaata tttttgtttt tccaaacgat caggattgtt acgtttgttt ttgcttgtta
    10261 gatagttacg ttctttacaa gaagtacact ctaaagtgat gtttacgcgc atgtttttcc
    10321 ctcccatttt tacaagaatc ctctaagatg actctcagac ttgattatct taccatgatt
    10381 ttttctagat tgctagtgtt tttcacgatt ttgttaagta aaactacttg acgataaata
    10441 caacggtttt acttctcaaa caaaatcttt ttctttttgt ataaataccc ttacacttgt
    10501 ttatatagat tatgtttttt aataaagggg gatactaata tgccatggaa catgaaggac
    10561 tttcctgcat caatgaaaaa cttagataaa ttgacaagaa aaaaagcaat cgacatcgca
    10621 aatgcattat tagatgaagg gtaccctgat aaccgtgcga tcccaattgc cattgatcaa
    10681 gcaaaaaaat ggtatgaaga tgctagtgaa tcagaaaaac gaactttcga aaaagaaaaa
    10741 aatccaagca agacggatga acacgatacg aatcctcgtg ctggaaaact gcttgattca
    10801 gatgtgatcg tagaatatga ggaagaacaa tggatcgtga aatctaaagg ggcaaaaaag
    10861 gcaagcaacc attttgatac aaaaaaagaa gcaatcgaaa aaggaaagca agtagcacaa
    10921 aacaaagaat caactttgtt catttataaa aaagatggga caaaagaaaa agaaatcgat
    10981 tattgaacat atgtaaaggg gttgtgacga aaatcgtgtc acaaccccct tatgttcgaa
    11041 acaatggtgt tcctctgcca actccgaacc ataagtcata atatctataa aacgggaaga
    11101 gctgtttttt gaaattgttt cttatcactc agtgttaaac ggtaggagaa aacctctttt
    11161 ttattgatat ttgattttca tcaaacagtc acatagtcga ttgcttctgc ttgcggaatc
    11221 gtttcatcta caaagtagac tttataccat gtcaagaaac tgaagattgt ccagattttt
    11281 ctttgctctt ctttacgtcc ttcgtgatgg tcatctagca acttcatgat tttttcttga
    11341 tcaaaaaatt cttttgcaaa atctgcttcg aataatgctt tgacttgttc gtagccgtgt
    11401 tcttctttca accaagcttt gattggcaca gggaatccta atttcacacg attcgaccat
    11461 tcttccggaa gatgtttgtt cgcagcttgt ctgaaaatat cttttgtgtt gttttgattc
    11521 aataaaaact tacttggtat ttgttgagcc aatttcatga cttcgatatc caataaaggt
    11581 actcggactt ccaaagaact tgccatagaa agtttatctg cctttaacaa gatatctttt
    11641 ggcatccatt gatgaagatc gacatattgc atcttattga cttcatcttt gattccttct
    11701 gttttctcgt aatgaacagt catgatttca tccacagatg gtgctgtttg atattttggt
    11761 gttaacactt ctgatgcttc tgattcttcg aacactcgag catgaccgat aaaatattct
    11821 tttgcaggag caagtgattc atataaatgg atccgtccat ggaagttttt catttttccg
    11881 attttacgtc ctaaattata gcgtgttcct tttggtaatt ttttcaatcc ttgagcaatc
    11941 acacggatga attttgaatt cgtatggaat ccatacgttt ggtatcccgc aaacagttca
    12001 tccgcccctt ctccagattg gactacccgc acactttgtg aagcaagatt tgctaaaaag
    12061 tacaaaggta cgcaagaagg atttgaatca ggttcatcta agtgatactg gatcaaaggg
    12121 aatgctttga acgacatgtc tccgtcgatc actgctgctg tattatctaa gtccaatagt
    12181 tcggttagtt ttctggcttc aatcgcttca ttataggttt tatcatcaaa cccgattgag
    12241 aaagaatgat ctggtttcaa tacagaggtt acataactgg aatcaacccc gctggataag
    12301 aaagaaccaa cttctacatc actgaccgta tgtgcttcaa tagaagcttg aacggtttcg
    12361 tcgatttttt cgatccattc ttgtcgggaa tgcgtttctt ttgtttcaaa atctgcatcc
    12421 caatattgct gaatgtctat ttttccatct ttatacgtat aataatggcc ttcaggcaaa
    12481 cgatagacat ctttgaagaa tgtttcgccg tttaatggag aatattggaa agtcatataa
    12541 ggtttcaacg cttctttgtt caattctttg ttaaaatcag gatgaggcag aaaacttttg
    12601 atttctgaac caaacataaa tgttccgttc atttctgcat aataatatgg tttgatccca
    12661 aagtggtctc ttgcaccgaa taactcattc ttttcattat cccaaatagc aaaagcaaac
    12721 attccgcgga tcttttgcaa taattctgtt ccccattctt cgtaaccatg caaaaggact
    12781 tctgtatctg catgtgtttg aaaaacgtgg ccagctgcaa tcaattcttc tctcaaaggc
    12841 tggtaattat agatttcgcc attaaaaata atgatctttg tccgatcttc attgtaaata
    12901 ggctgagtac cgccctctaa gtcaataata cttaatcttc tgaatcctaa agcaacatgt
    12961 ttatcgatat attctcctga actattaggt ccacggtgaa cgattcgatc catcattgta
    13021 ttaataattg tttttttgtt gtccttgtcg ttaacaaaac ctacaatacc gcacatttaa
    13081 ctttcacatc ctaatataaa attactgttt atttcattca tatgcatctt atcatagaat
    13141 aaatagcgga cgaatatttt ctctcttaca atttcgtcac gtgaaaacaa aagatgaaag
    13201 tttctgtttt tacagaaaat aaagtcccaa tatctaattt accatgtttt cttttttgag
    13261 gctaccttat tctcttcgtg ttaaaaaaat attagtggat gctatttcca aaaaaaccag
    13321 caaaaaaaga cacgacctaa agatcgtgt
//
LOCUS       DAZUMB010000071        13172 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000071 DAZUMB010000000
VERSION     DAZUMB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.072"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..1800
                     /gene="yfcC"
                     /locus_tag="ACG80L_001794"
     CDS             61..1800
                     /gene="yfcC"
                     /locus_tag="ACG80L_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS5024020.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1957..3141
                     /gene="iadA"
                     /locus_tag="ACG80L_001795"
     CDS             1957..3141
                     /gene="iadA"
                     /locus_tag="ACG80L_001795"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS5024021.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3252..3455
                     /locus_tag="ACG80L_001796"
     CDS             3252..3455
                     /locus_tag="ACG80L_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5024022.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3556..4218)
                     /locus_tag="ACG80L_001797"
     CDS             complement(3556..4218)
                     /locus_tag="ACG80L_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024023.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4231..5733)
                     /locus_tag="ACG80L_001798"
     CDS             complement(4231..5733)
                     /locus_tag="ACG80L_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5024024.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6281..7960
                     /locus_tag="ACG80L_001799"
     CDS             6281..7960
                     /locus_tag="ACG80L_001799"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5024025.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8087..8566
                     /locus_tag="ACG80L_001800"
     CDS             8087..8566
                     /locus_tag="ACG80L_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS5024026.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8785..9465
                     /locus_tag="ACG80L_001801"
     CDS             8785..9465
                     /locus_tag="ACG80L_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS5024027.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9473..10804
                     /locus_tag="ACG80L_001802"
     CDS             9473..10804
                     /locus_tag="ACG80L_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS5024028.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10819..11781
                     /locus_tag="ACG80L_001803"
     CDS             10819..11781
                     /locus_tag="ACG80L_001803"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS5024029.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11892..12230
                     /locus_tag="ACG80L_001804"
     CDS             11892..12230
                     /locus_tag="ACG80L_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS5024030.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12309..13016
                     /locus_tag="ACG80L_001805"
     CDS             12309..13016
                     /locus_tag="ACG80L_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS5024031.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4181 a         2331 c         2896 g         3764 t
ORIGIN      
        1 catttgatta tccatttttt attcatcaaa attactgaaa aaagaaagga agaaataaat
       61 ttgaagaaaa caaattcttc aggaaaaccg aaacgcgact tcactaaatt atcgacaccc
      121 catacgtatg tcattatttt tggtgtagtc atctttgcat ggatactgac ttttgtcgtt
      181 ccagcaggaa agttcagcac gcaagatatt gaatacaaag atgcaaatgg cgaaacaagt
      241 actcgtactg ttttacgtca agattccttc cgttatgctt acgagctgga taagtcgtat
      301 gtgtttgatc aattagaaga actgcaagat catccagcag aacgtgaaaa actggatgtt
      361 ccggaaaaag gattggaaaa agtcattgct gacggagaaa aaaatcttac gcaggaaaaa
      421 ctagatgaaa tctccttgac agatgatgtg ctttacgatc agtacggcga aaatatctat
      481 gatacttcta aaaaattgca taaaacagcc aaaatatggg gaacagatga ttttggcggc
      541 tttggtttct taaattttgt ttttgaagga ctagtatctg gagataagta tggatctgct
      601 gtagggatcg ctgcattgat ccttgttgtt ggaggtgctt ttggtattat tatgcgtacc
      661 ggagcgatcg acgcaggtat ctatgcgttt atcagtaaaa ctaagggact ggagcgttta
      721 gctttaccgc tattattctt tgctttttca ttcggtggcg ctacattcgg gatggcagaa
      781 gaggtaattc cattctcaat ggtgatggtt ccatttgtca ttgcgttagg atatgactcg
      841 atcgtagcag taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac ctcttggatg
      901 agcccgttta gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt gtctggtgcg
      961 acattccgat tgattatgtg ggtagttgtt actgcattag ccgctggtta tatgatgata
     1021 tatgcagaaa aaatccgtaa aaaaccagaa agttcgttga cttacaaatc agatgattat
     1081 ttccgttctc atatcaaaaa gacatctgat gaaaataaaa aattcatgct tgggcataaa
     1141 ttgatcttat tagagatgtt agttgtcctt gtctggatcg tttggggcgt gactcaaaaa
     1201 ggctattaca ttccagaaat cgcttctcaa ttctttgtga tgggattagt tgctggaatc
     1261 attgctgttc ttttcaaatt agatggaatg aaagtcaatg acatcgcttc ttctttccaa
     1321 tctggtgcag ctgatttagc tggtacagca atcgtagtag gtatggccaa agggattctt
     1381 cttgtcttgg gaggatctga tgcatctgtt ccttcagctt tgaatacgat tttgcatggg
     1441 atcgggacgg cgttgaccgg tgttccagct gtcattggtg cttgggcgat gtacatcttc
     1501 caaagtttgt tcaacttggt tgttacatca aattcaggac aagcagcttt gactatgcca
     1561 atcatggctc cactggcaga tttagtaggt gtatccagac aagtagcagt tcttgcttat
     1621 cagcttggtt caggttttat ggacgctttt acacccgtat cagccagctt gattggtgtg
     1681 ctaggtgttg cacggatcga atgggcaaaa tgggcaagat tccaaatcaa gatgcaagga
     1741 ttcctgtttg ttttaggaac tattttcatc atgattgcca ttgcaatagg tctgcagtag
     1801 tactggagac ctgtaaataa aaaagtaatc caaacagtag ataaggactc caagaaccgt
     1861 cattttacgg taaaatcaaa ggagtaaaga tcctatcgtt tggattattt tttaagatcc
     1921 tatcatcagt tatacttaaa gaaagaggaa aaatgtatga aaatcattcg acaaattgaa
     1981 gtgtttgcac cagattactt aggaaaaatg gatgtactga ttgccggtgg gaagattttg
     2041 gctgtagaag aacagctaga aggcggctac gaaggagtgg aagtagaaga actttcaggt
     2101 gagggaaaag tactaacccc gggtttcatt gactgtcatt tccatatttt aggtggagga
     2161 ggagaaggcg gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt gactacagct
     2221 ggtgtaacga cagttgtcgg ttgtttggga acagacggag aaggtagaga tatgactgcg
     2281 ttgatcagca aagcgaaagg tctggaagca gaaggaatct ccacttatgt atacgaaggt
     2341 tcgtaccgat tgccggtaaa aacagtaaca gattctatca tcaaagactt tttgacaatt
     2401 gataaaatta tcggaatcgg agaaatcgca gtatccgatc accgttcttc tcagccaagt
     2461 tttgaagagt tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt atccggaaaa
     2521 gctgggatca tcaatgtcca cttaggcgga ggaaaaagaa agctagagct attgacacga
     2581 atcgtagaag aaacagaaat tccgatcaca caatttttgc ctactcatgc aaatcgcaca
     2641 ccagaattat ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat tgacttcact
     2701 gctagcgaag atcctgattt ttgggagaag acagatggtg aagttcgctt cagcaaagca
     2761 ctgaaacgat tgatcgagga agatgtttct ttagataatt tcacgatgac ctcagatgga
     2821 caaggaagct tgccttattt tgatgaaaac aatcatttcc tagggttagg agtaggaagt
     2881 gccaaggcat tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat ccctttggaa
     2941 atcgccttac gagctataac cagtaatcca gcacgtatat tgaaattgga taaaaaagga
     3001 aaaatcgaaa ttggtgctga tgcagatctg tgtatcttgg ataaagaaac gcttgatatt
     3061 gatacagtta tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa agtatggggg
     3121 actttcgaaa aaagttttta atcaacagct ctaactatct tttttagaaa acgagcgttc
     3181 tgtttgtctg tatctgttgt tgatgttacc ctgaaggtag atcaataaac aaactaagag
     3241 gaggaagcac aatggcagat ttaaaaggac gttctaaaga tatgaaagat aaagtagtcg
     3301 gtgaaggaaa agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa acagaaggga
     3361 aagcacaatc actaggtgct gacatcaaag aaaaagctcg aaatttaggc gacgaagtca
     3421 aagacaagtt ttctcgaaaa gacgaaaaaa aataaaaacc acttattact aattgaatca
     3481 agcaaataaa aaatccgaac catatctgcg agacagatat ggttcggatt ttttatttga
     3541 tcattatcct gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac
     3601 ttcatcacag ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg
     3661 ttcatgggcg atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt
     3721 tccagtaggc tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc
     3781 gactcgctgc tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc
     3841 aattcctaca cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt
     3901 agtgatttcc attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta
     3961 agcttggaaa ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag
     4021 tgaacgatct tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga
     4081 aagaaaagtc gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc
     4141 gaaattcagt tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa
     4201 ttgttctgtc cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa
     4261 tcttaatatc cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc
     4321 ggctaatagt cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga
     4381 aacagcaaat ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg
     4441 tctgttacct ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt
     4501 ctgttggtca agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc
     4561 aactcctaat gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt
     4621 acttctggat tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt
     4681 aatcataatg atcagagtaa gtatgataat accggctaca gccactagta aaacaacatt
     4741 tttagaaaaa cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt
     4801 tgattgtaag ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc
     4861 cattttatct ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt
     4921 cccatttagt tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa
     4981 gttgaatttc atacccatac tgccgcttgt ttcgctggat tcataaattc ctttgatcgt
     5041 catttcataa gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc
     5101 caggtcattt gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct
     5161 gtcatctatt ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg
     5221 tgcagttgct gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc
     5281 cattcctcca gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc
     5341 agaactagtg atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga
     5401 ataactcttt acattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt
     5461 aagagaaaaa ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg
     5521 ttcctgtttt ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc
     5581 tttttgtgct tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac
     5641 aaaaataaga atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt
     5701 tgtacttttc catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat
     5761 ttgataatag acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca
     5821 acatgaggtt ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg
     5881 aaaaaagtat gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct
     5941 agatgctaca gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat
     6001 ttagtttatt tgttgtataa taatggggaa cagctgaata cttatataag tattctggct
     6061 atagcgtttc gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc
     6121 caactgtcag aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa
     6181 tagtgatttt gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg
     6241 acatctttaa acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat
     6301 cccattaggc ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga
     6361 tatttttatt ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt
     6421 cgtaatccct gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt
     6481 aacacatgga catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt
     6541 accagtattc gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga
     6601 agcagccaaa ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg
     6661 tgaaacagta gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa
     6721 tctgaaaaca aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc
     6781 tatcccaatg tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt
     6841 agccttacta agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca
     6901 aatagcagat gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg
     6961 tgtagcaagt aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg
     7021 aaaagttgtc ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga
     7081 aaaacttcaa ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga
     7141 tccagaagaa ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca
     7201 aaaaatggca aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat
     7261 cactactacg ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta
     7321 tcgagctggt ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa
     7381 tcctaatgac ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca
     7441 aggagagtat cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata
     7501 caaaaatatc tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag
     7561 tgttgcagga actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat
     7621 cggaaacatt gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt
     7681 tgtcactatc agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg
     7741 attcgtttac gtaaaagcta gccgtgatct gatgaaagaa agcagcaata tgattgaaac
     7801 aatcgttgag aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat
     7861 ccgagatcaa ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc
     7921 agtcattatg gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac
     7981 ctctctatag agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt tttgaataca
     8041 gcatccctcg ctttataata aacgatagaa taaagggagt gaatcaatga tgagtcaaac
     8101 aaaagtcgaa cgttatttag aagaacaaaa gatcagctat caaccacttg attttagtca
     8161 tgtggtagaa gaatctttca cagaagaact acaaaaaaga gggattgatc cagctttgat
     8221 atgtaaaact ctcgtagtga aaggaaacaa aactggggtg atcatcgcat tgattccttt
     8281 gaacgatcac ttagattaca aaaaaacgag acaagtgacg aaagatcgta aagttggttt
     8341 tcctggaatg gattttgtat taatgcatac aggatatccc cacggagcaa acacccctat
     8401 tggaataaaa ctcgcacatc ctgattattg ttttttagca gacagttctt taaaagaaaa
     8461 agaagaagta attgtgtcaa gcggtgaaat cgggagaagc cttcggataa aagtagccga
     8521 tttagaaaag atcgtccatc ctatttacgc tgatttaatt agttaaggga cgagaagaga
     8581 agctaagcag agcagagacc tcaaacttga gattatatct gcttggtact tggtgcatcc
     8641 tgtattatct gttacaattg ctttgtacat actgtactat caatccaatg aaaatatatt
     8701 tagcagctat attttttcat tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata
     8761 cagagaaaac gcgaggtgta aaatatgaaa attacgtatc atggacattc ctgtttaaca
     8821 attgaaatgg aaaatggcca acgcgtgatg attgacccgt ttattacagg aaatccattg
     8881 acagacttaa ctgcagaaga agtaaagaca gattggttgc tgattactca tggtcatagt
     8941 gaccacatag gagatatgat tcctattgca aaagaaaatc aagccacaat tatcagtatt
     9001 gttgagattg ctgattatgc gaacagcaga ggagtagatt cattcggtat gaatatcggt
     9061 ggaaaacatg cgtttccatt cggcgtggta aaattcgtac atgcacagca tagttcaagc
     9121 tatgaagtag acggtatcgt tcagtatatg ggcgagccta gcggaattat tattcaagca
     9181 gaaggaaaaa cgatttatca tgcaggggat actgcttatt tcagtgatct aggattgctt
     9241 gcagaagaat tcgatattga tgtcgctttc ttgccgattg gcgataacta tacgatgggg
     9301 ccagaagatg cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt tccaatccat
     9361 tacaatactt tccctgtcat tgaacaagat cccgaatctt ttgtagagtt actggaagat
     9421 agtgaaggaa aaataatgag cgtctcggaa acgctggaag tgtaggcaga aaatggcgac
     9481 aaaacatgat ttgattttag aatatattga aagtttacct gttggcaatc gaatttctgt
     9541 aagaagcatc gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga
     9601 tgcagaaaat gtcggactag tttcaacaat tcaacgagta gggaccatcc gtatcgaacg
     9661 caaattaaaa aaacatattg aacgtttgac atttggcgag gtcgtcagga tcattgaagg
     9721 ggacgtttta ggcggggcgg caggattaga caaggtcttg aataaattcg tcatcggcgc
     9781 gatgactaaa catgccatgg aacgctatat tacgccagga tcactaatga tcgtcggcaa
     9841 ccgggcggaa gtccaaaaat tagcattgga agatggcgcc gcagtattga tcacaggagg
     9901 attcaatacg actgctgaga ttgcaaagct agctgatgat cttgaaatgc cgatcttacg
     9961 cacctcttat gatacattta cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt
    10021 gatcaaaaaa gatatcatgc ttgtcagtga tatctatatg ccagctgaaa agactcatta
    10081 tttgcagcag acagatacga tttctgatta tcagcgtttg tcagaagaaa cgcagcattc
    10141 gcgttttcca gtcgtcaacc gccatcatcg tttgatgggg attgttacgg caaaagatgt
    10201 tttagggaaa tcgccaaacc agatcatcga tcgggtgatg acgaaagaac caatcagtgt
    10261 aaagaaaaca atgagtattg cctctgttag ccatcaaatg atctgggatg ggctagaagt
    10321 catgccggtc gtttccgatg acttgacatt ggaaggcttg attacccggc aagatgtaat
    10381 gaaagcgatg cagcttgtcc agcgacaacc gcagattgca gatacgattt cggatcagat
    10441 atctggaaat atccatacga ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa
    10501 gttcaaattc gtagtagcac cacaaatggt caatggcgtt ggaaccattt cttttggcgt
    10561 acttagcgaa atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa
    10621 tatcttgatt gaacaagtga acctacatta tttgcggctg atccaattgg aaagcgaatt
    10681 agacattcgt ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg atatcgaagt
    10741 gtttattgaa aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc
    10801 ttagaaagca ggagtgaaat gacagtacag caagaaatag tagatacaat aaaaaaatac
    10861 gataaaatca ttatccacag gcatttgcgc cctgatcctg atgcattagg atcacaagta
    10921 ggattagccg aactattacg ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa
    10981 acgattgaag ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt cttctaccaa
    11041 ggagcgctag tcatcgtgac tgatacagct aattcaccca gaatcagtga tcaaagatat
    11101 cagttggcag aaaaactgat caaaatcgac catcatccca atgatgaccc ttatggagat
    11161 cttgtgtggg taaatacaaa agcaagtagc tgtagtgaga tgattgcaga atttgctttg
    11221 atgtttcctg aatctttaaa gatgaacgtt aaggctgcac ggttacttta tgcaggaatc
    11281 gtcggagata cagggcgctt tttgtatccc tccacgacgt cccgtacatt agagattgct
    11341 tcgatattga gaaattatcc atttgatgca agtgctttga acagagagat tgaacaaatg
    11401 ccgatgaaag tcgctaaact ttccggttat ctttatcaaa atatccaggt ggatgaaaat
    11461 ggtgcaggaa gaataatcct tccgcaagaa attcttgata aatatggaat cgatgattca
    11521 gagacagctt cgatcgtttc acttcctgga gtgattgacg aagtgctggc ttgggggatt
    11581 tttgttcagc agccttcagg gtattaccgc gtacgtttac gttctaaggg gccgatcatc
    11641 aatgaattgg ctaaacgcca tcatggagga ggccatccat tagcaagtgg tgcgaatgca
    11701 aaagatttag cagaagtaga ggaaatctat aaagagatcc aagatatatg ccaatcgtat
    11761 aagagcagaa aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac
    11821 aaatgtatct tttcaagatc ttctttttcc gttacaatag tgctcactaa agattttaaa
    11881 ggagaaagaa gatgacatta ccaaattgcc cagaatgtgg ctcagagtac acatatgaag
    11941 atcgaggctt gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa
    12001 ctgtcataga agacggatta gtggtattag atgcaaatgg aaaccgtctg ttagagggcg
    12061 atagcgtgac agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag
    12121 gaacaaaagt caagaacatc cgtttagtag aaggcgatca taatatcgat tgcaaggtag
    12181 aaggatttgg accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa agaatattca
    12241 aggtcttttc acattctttt cctactacta atgataaaat aaagtaaacg aaaaggagtg
    12301 aaacgaaaat gtatggttat ggaatgtatg gttttttaga tccgacattt cttttggtca
    12361 ttcttggatt agttatttcc ggaattgcgt cagcgtacgt caatagtact ttccgaaaat
    12421 acgatcaagt gagaagcaaa aacaatgtga ctggtacaca agcagcacaa tatatattac
    12481 aaagtcaaca gataaataat gtcggtgttc aacaaattgc cggagatctc acagataact
    12541 acaattcagg taataagatg ttgagtttgt ctcaagcaac tgcacagtct acttcagttg
    12601 ctgcgatagg tgtagcagct catgaatgcg gacatgcggt acaagatgct tcgggttatg
    12661 tcccattgaa gattcgtgca gcacttgttc cagcagctaa ctttggttcg atgatttcgt
    12721 tccccttgat tatggtaggt gtattgctta gctggaactc tacgttaatc aatatcggga
    12781 ttttcgcttt ttctttagca ttacttttcc aattggtgac attaccagtc gaatttaacg
    12841 cgtctcggcg agcaatccaa attttgagtg aaggcggact actgacggaa gaagaagtac
    12901 cgatggcaag acacgtatta tttgcagcag cactgactta tgttgcagca gcgctttcga
    12961 cattcctgca gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga gattaaaata
    13021 aagaagttgt gaaatcagcg ctttgatctg agctaatacc gagagttagc tgattgaata
    13081 ccatttattt ggaataaggg ctctttgtca ataaggactg atgggttgta caaaatcaaa
    13141 tctgggtcag aatgaacctc attctggctc ag
//
LOCUS       DAZUMB010000072        13111 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000072 DAZUMB010000000
VERSION     DAZUMB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.073"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2085
                     /locus_tag="ACG80L_001806"
     CDS             <1..2085
                     /locus_tag="ACG80L_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024032.1"
                     /translation="SSYLTDKKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKH
                     PLTYTDYSTYSEIRKELNFIETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQ
                     QKYINNKNNLFWQKKNKGIEMVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILS
                     IENEVGNLFTTQTVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLE
                     VDSSAIGAMIRNLRIGLVAVSLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFS
                     GKELDLVAQSVSRIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFG
                     YSSQNDCYYTALVQIDLLEDSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQA
                     TIYRMDKDDAEASKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKE
                     ALYYRVNTGPSSIIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELI
                     DALFAQNKNPLSREVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELE
                     KLVLEQLVLPITECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYL
                     SNVFKKETGEKFGDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKE
                     SITPGEYRKKYL"
     gene            complement(2667..3539)
                     /locus_tag="ACG80L_001807"
     CDS             complement(2667..3539)
                     /locus_tag="ACG80L_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5024033.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            3839..4813
                     /locus_tag="ACG80L_001808"
     CDS             3839..4813
                     /locus_tag="ACG80L_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5024034.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            4835..5527
                     /locus_tag="ACG80L_001809"
     CDS             4835..5527
                     /locus_tag="ACG80L_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5024035.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            5493..7001
                     /locus_tag="ACG80L_001810"
     CDS             5493..7001
                     /locus_tag="ACG80L_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5024036.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            7075..8178
                     /locus_tag="ACG80L_001811"
     CDS             7075..8178
                     /locus_tag="ACG80L_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5024037.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            8181..9518
                     /locus_tag="ACG80L_001812"
     CDS             8181..9518
                     /locus_tag="ACG80L_001812"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS5024038.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            9505..10383
                     /locus_tag="ACG80L_001813"
     CDS             9505..10383
                     /locus_tag="ACG80L_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS5024039.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10380..11303
                     /locus_tag="ACG80L_001814"
     CDS             10380..11303
                     /locus_tag="ACG80L_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS5024040.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11300..11581
                     /locus_tag="ACG80L_001815"
     CDS             11300..11581
                     /locus_tag="ACG80L_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5024041.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            11585..>13111
                     /locus_tag="ACG80L_001816"
     CDS             11585..>13111
                     /locus_tag="ACG80L_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS5024042.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     A"
BASE COUNT         4389 a         2176 c         2785 g         3761 t
ORIGIN      
        1 tcgagttatt taacagacaa aaagaatttg ctcaggcaag ttaaaactaa cgttgaatgg
       61 aaaattagaa cgattgaata ttcgttttct acgtatggtt cgaccaaaaa tttctcggac
      121 atattcaagc atccgctgac atatacagat tactccactt attctgaaat caggaaagaa
      181 ctaaatttta tagaaacgat tgttatggat gataataatt atgatttagt cagtttggct
      241 ggtaaatggg gagtaatcaa tggatcgttg agtcaactga ccgaagagga agttgaaaag
      301 tatcaacaga agtacataaa taacaaaaat aatttatttt ggcaaaagaa aaacaaagga
      361 atcgaaatgg tgatttcttt gccaatgttt gaaaaagaaa aatatgcatt gggaattgct
      421 acgatagaga aacatacaat agaacaagtt gttggtgaaa gtggagaaga catactttct
      481 attgaaaacg aagttggcaa cctttttact actcagacag taaaaaataa agagcaacca
      541 actgtattaa cggacgtgga tctagattac aatcagctga aagtgataag agaaaaagat
      601 aaaacgtata tagtactgaa atcagattat aataattgga tttacagatt agaagttgac
      661 tctagtgcaa ttggcgcaat gatccgcaat ctgagaatcg ggctagttgc agtgtcttta
      721 acactggtag gactcattgg tcttttttcc tacttatttt cggacagatt tgcccgtccg
      781 atttctcaaa tacaggaacg tctgaatctt aaatcaaaag gattcagtgg gaaagaacta
      841 gatttggtcg ctcaatcagt cagtcggatc ataggagaaa aagaagcgtt aagtgcacac
      901 ttagttaccc aaaagccaca gctggaaacg ctatttgtac ttagtttatt tagaaatcga
      961 gtagaaaggc gagaactaga tcagcggttg cagcaatttg gttatagttc tcaaaatgac
     1021 tgctattata cagctttggt tcaaatcgat ctgttagaag atagtcacgg tggggagcgt
     1081 gatttgctac ttttagcgat caacaacatg atcacggaaa ttgtgccaaa ggaaaatcga
     1141 atgctgccga tcgttttgaa tgaagaaatg caagccacta tttatcgaat ggataaagat
     1201 gatgcagaag caagcaaaaa agtcatggag tattgccgac tcattcaaaa acgtgtaaaa
     1261 gaatacttga atttaactat cagtataggc atcagtaacc ggttcgaaac gctgaatgaa
     1321 agcaagcaat cggttgatcg ggcaaaagaa gccctgtatt atcgtgtaaa tactggtcca
     1381 agttcaatta ttttttatca ggaaatcgtt cctgctgctc atgaaaaaac acttattaga
     1441 tatccgatag aagaacaaaa tcgattgttt gaagccattc gttctggaaa tcaggaagtg
     1501 aagaatttag tacatgaatt gattgatgca ttgtttgcac aaaataaaaa tcctttgagt
     1561 cgggaagtag tttcggtccg cttgatcaat gaactcgttc aattaggaca gttattggga
     1621 gtagattcaa aaaactttga taacatgaaa caaatttatg taaaagcact gaacaattat
     1681 catcccaaag aactagaaaa acttgtttta gaacaattag ttctgccaat cacagaatgc
     1741 tctcaagcaa tcactgatca tgaatttaaa aatttgtcgg agaatattat gcatatcgtc
     1801 tacacagaat atgatcgtga tctgtcttta gatgtgatcg ctgataggtt gcattataac
     1861 cctaactatt taagtaatgt atttaaaaag gaaacaggag aaaaatttgg tgattttgtt
     1921 caaaatcatc gtttagaggt agcaaaaaaa tggttgaaag agactaacct ctccgtaaaa
     1981 gagattgcag aaagattgca atatcgaaat ccgcaaaatt ttattcgttt ctttaaaaag
     2041 aaggaatcga ttacgccggg agaatatcga aaaaaatatt tgtgaatcaa gaggctgttt
     2101 ttcttgtgat ctgattcaag caataaaaga ggacagcaaa aaacagcact tcatgttttg
     2161 ctgctggctg attttattgc cgcagaatca acaagataaa cgccgtttaa tcaagaggct
     2221 gtttttcttg tggactgatt caagcaataa aagaagttag aataacccta gtatttgtat
     2281 ttatggatag ttaaatttca aggctaagca cgcaaaaaga actgtgacaa aagtcttgtc
     2341 acagttcttt tttacgaata aatggcgttc aagagacagg ctatcgacaa taaggcataa
     2401 ttttgtgaaa tatgggaagc tatttttcaa aatcgcttct tattgctcct gtgggttact
     2461 cgtcgcaatg tttgtgagag tttttatcat cgctcaaggc tgaacgtctc tttcacaacc
     2521 tctgatacac ggtgttccag gagtagattc ttcgaaaaat aagccgaaat ttcacaaaaa
     2581 tttaagaagc aattttcgta aatgccttct tatttcttga agctgaccac ttctgacaca
     2641 actaccattt tgcgttcagt acataatcac cctttgacag aacctacgag tgctcctttg
     2701 acaaaatatt tctgaacaaa aggataaaca atcagcatag gcactgttgc aataacgata
     2761 acagccattt taactgtttg tgctggtgga atcacatcaa cggcactact ttctgcttgc
     2821 atcccacttg atacaatgac gatttggcgt aaaagaatct gtatcggcca tttagatgaa
     2881 tcgttgatat acaaaatagc agtcatataa gtgttccagt aagtaacagc ataaaaaagc
     2941 gagatagtag caatagacgg taaagctaat ggcagcataa ttttgaaaaa tacaccaaaa
     3001 tctgtgcaac catccatttt tgctgattct tccaaactat ccggcaaggc ttggaaaaag
     3061 tttctcataa tgatcatatt cattgcatta acagccacag gcaggatcat ggcccagtaa
     3121 gaattgacca gccctagaga acgaacaact aagaacgttg gaatcatccc cccgctgaac
     3181 aacatagaaa atacaacaag aaagttgacg aaacctcgac caaacaaata ctttctggaa
     3241 agaccgtagg ccatcagtga agttgcacac atactgacga tcgttccgac acctgtaaca
     3301 ccaatcgaaa cagcaagtgc tctaaaaatg gtcggcgtag aaagaatgta gcgatacgcg
     3361 tctaaagaaa aggtagtagg aaaaaggata aactttttcg cgacgacttc ttgtgtagaa
     3421 gcaaaagatg tagcgatgat atttaaaaaa ggaacgatac agatcaaagc tagcaaaatt
     3481 agaaacgtat tgttgaaaag tgtaaaactt ctttctccta tagagacttt ctttttcata
     3541 tagataggct cctcctcaaa ataaattaaa tgtttctgtt tctatatgtt caatttatca
     3601 tcggtgaaag attacgtata taatcatttc ctatgctaag tgtcagatag aaaaaaatag
     3661 aaggtttgct atgcgtagtt tatttgtgaa aaaatacact ttatcgtttt gtatagcaca
     3721 aaaatcattg ctatgtaagc gtttttactt acgataaaaa tgattatata cgtaattcag
     3781 atagttatct atcatgaagt cataagcagc aaacattcac aaaactaagg aggggcttat
     3841 ggaaagtgta aagaaacaag aaccatttca actgacgcta tcgaaaaaag aagaaaagct
     3901 cagaagacga aaaaaaataa ttgcaggaat caaaacgaat aaatttttat atgtgatgat
     3961 tctgccggga gtcctttatt tcttgatttt taggtatctt cctatggggg gactcgttat
     4021 tgcttttcaa gattatcaac catttttagg aataatggga agtccttggg tcggtttcaa
     4081 acatttcatt cgtttgttta cagaaccgac attctttaca ttattaagaa atacgttgat
     4141 cttgttcgga ttaaatatct tcgtgttttt tccgatgcca atcattttgt ctttactatt
     4201 aaatgaagta acgaacaaac gattgaaaaa cgggattcaa acgatcattt acatccctca
     4261 cttcatgtct tgggtaatca ttgtttcgat tacttatgta tttttgaatg ttgacggagg
     4321 ggtattgaat gaattccttg caacaatggg atttgaaaaa atcagttttc taacatcccc
     4381 tgaatggaca cgcacagtat atatcggaca agtgatctgg aaagagttgg gctggtctac
     4441 gatcatctac ctttcagcga taactgcagt cgatacacag ttgtatgaag ctgcagaaat
     4501 ggatggagca ggacgcttac gcaaaacatg gcatgttaca ttgccggcaa ttcgtccagt
     4561 aatcattacc ttgctgattc taaaaatcgg tcagacatta gacttagggt tcgagcatat
     4621 gtacttgtta ttgaattcat tgaatcgaag cgttgcagaa attttcgaca cctatattta
     4681 tacagctgga ttgaagaatg gacagttaag tttcagtaca gcaatgggat tgtttaaagg
     4741 ggtcgtagga ttgtttatgg taattttagc taataagatt gccaaaaagc taggagaaga
     4801 cggcgtttac taagatagaa agagagggaa agatatgctg cagtcattag ataaaataaa
     4861 cggaaggcta aatcgtattt tacagttggt ttatttgaat tttctgtggg gcggtttagt
     4921 catcgcaggt ttgggagttt ttactttcgg tcctgcatcg tatgcgatgg ttgcagtgat
     4981 tagaaaatgg ctgcgctcaa aagaaacgtt tcctttgacg aaaacgtact tcacatattt
     5041 taaagaaaac tatcgtgaaa ccttgctgat gagctggata tatggcgcca tcgggattgt
     5101 tttacttgtg gatctttctt tcatacacaa ttggtatctt aggatcgctt tgtatattgc
     5161 tgcgttcata tatgtgctct cagcactcta tattttccca atcatggctc attatgattg
     5221 gaaaggatat tttttcaaaa tgaaaatggc atttttattc ggattctcac atcttcatta
     5281 ctcattagtg ttgatattaa gtatggtagg gatttatctg atgatcatcc aaacaatgcc
     5341 aggtgtttta acctttatcg gtggcagtct cttcttttcc gcagtcacgt ggacagcaaa
     5401 ttctatattt tgccatttgg aatggaagaa ctcatataca gaaaaaacag aagaaaacaa
     5461 aaatattatt aaaacagtga aggagaaaat ttatgaaaaa agcaacgctc gtaaaattag
     5521 gtcttagttt tgcaggaatt ctagcacttg ccgcatgcgg cagcagttca agtaacagtt
     5581 cttcagagac agaagatgga aaagctaaaa tcacttggtt aaatattttg catcatgctt
     5641 caccgcctac agacacgatt gtgaacttga tcgaagaaaa aaccgatacg gaactgacat
     5701 tcaactggat tcctgatgct tcaaaagaag aaagattaac aactgcattg gcttctgatg
     5761 aactaggaga tatcgttaca cttcaaattg cgatgttgaa aaactcttct gtccgcaatg
     5821 cgttaaaatc aggcgtattc tgggatgtat cagaatattt aaaagactat aaaaatttaa
     5881 gtcaaatttc tgaagataga atcgaagctg cgagtatcga cggtgccttg tacggcgtac
     5941 cgatacaaaa agactacgct cgtgctggct tagtcattcg aaaagactgg ttggataatc
     6001 taggactaga tgttccaact accatggatg agctttatga agtagctaaa caatttacag
     6061 aaaacgatcc tgatggaaat ggtgtggacg acacagttgg ttttggcgat cgtgcgccta
     6121 gtgatattcg ttataccagc tttaaacttt tcacttctta ttttggagca ccaaatggct
     6181 gggctgtaga cgataatggc aaattcactc cagaatttga tacagaagag tatatgaaag
     6241 ctttgaattt ttcaagagat ctatttgaga atggctattt ggcacaagac tttgcagtta
     6301 cccaaaagac tgatcagcaa gaacaatttg cacaaggaag aactggaatc tacactggaa
     6361 tggtggatat caagaatctg aagacgatgg gtcaaggtat tcaagatgat atggaattag
     6421 tgcctgtaaa caagatctca gatggtgaag gcggcggtta tcatgtttgg tcagaaggaa
     6481 atggtattgg cggcttgcta gcatttccca aatctcaagt aaaagacgaa gcacaattga
     6541 agaaattact tcaatttgtt gatgatcttt tagaagaaga tgtgtataaa ttgatgactg
     6601 gcggtatcga aggaacgcat tatgaattga atgatgatgg ttctatcgaa tttatcgata
     6661 tggatctttg gcaacaagag gtgcaaccgt tatcaagctc tcgtcctagt gaagtgacgt
     6721 tctctttcaa agatacagac cctgaaaaag aattctctaa tcaattgatc aaagaaaatg
     6781 aagaatttgc tgtaatggat ccttctgtgc cattggattc accgacgaac aatgaagtag
     6841 gatcagaaat tgaaaaaatc gtcattgatg ctacggtgca atacatcatg ggacagcttg
     6901 acgaagatgg attcaaaaaa gctgtagaag actggaaagc acaaggaggc gatcgaatca
     6961 cacaagagta cgaggaagca tacaaagcag cggcacagta atatagacga ttgggacaaa
     7021 tacttcttgg tatttgtccc aaatcctatt ttaattttta aaggagagca aataatggaa
     7081 aactggtcaa agaaattagc aaaaagtatc atggaaagaa cacctcgctt atatgaagaa
     7141 aagtggtata aaggaaaatg gtcttatgac tatggcgtcg tacttaaagg gttccaatta
     7201 ctttgggaac gaacccaaga aaaaatttat tttgatttca taaaagacaa tatcgactat
     7261 ttcgtgcaag aagatggaac gatccgtggc tacagcgttg aagaatacaa tatcgaccat
     7321 gtaaatacag gaaagctttt ctttttattg tacaaagaaa caggcgaaga aaaatataaa
     7381 aaagcagcag aactcctttc ccagcagttg gcaaaccatc cacgcacatc cgaaggagct
     7441 ttttggcata aagaaattta tccttatcaa atttggttgg atggcttata tatgggttct
     7501 cctttttatg ctgaatttat ccagaatttt agtgaagaaa aagattacca agatgttttg
     7561 agacagtttg aaatcagtta taaacatcta ttcgatccaa aaacaaattt gctgattcat
     7621 gcatgggatg aaaagaaggt ccaaccatgg gctgatccaa aaacaggtct atcccatcat
     7681 ttttggagcc gatcgattgg ctggtatttg atggctgcag cagataccta tgaattactt
     7741 catgaagaaa cagattgttc tttgctgaaa gaaattctag aaaagacact ggctgcatta
     7801 cttccatatc aagaagaaaa cagcgggaca tggtatcaag taacaacaga aacaaaacgg
     7861 aaaggaaatt atctggaagc ttccggcagt agtatgtttg tttacgcaat ggctaaagga
     7921 atacgtttgg gactgctgaa caaagaagaa tggcttccac aagtgaagaa atcacatcaa
     7981 ggattacttg atgaatttgt tctggaaaca aaagaaggct ggctgaactt gaataaaaac
     8041 tgtcaagtag cagggttagg cggagcagat cagcgagatg gttcatatgc ttactacatc
     8101 agtgaaccaa ttatttgtaa tgatcaaaaa ggcgttggtg cattcttgca agcattgatt
     8161 gaagtggagg cattataaat atgtatgata ttttgacatt tggcgcatca attgatgaat
     8221 tgaatactga agcaatacaa caagccattg atgctgcagc aagtgacgga ggcgggaccg
     8281 tagttgttcc agcaggtgaa tttctaacag gagctttatt tttaaaaagc aatgtcgaac
     8341 ttcatttaag cgcaggagcg gttctgaaat tctctgacga tccaaaagat tatcctgttg
     8401 ttcattcacg ttgggaagga gtccatcgaa aagtttatgc ttcctgtatc tatgctcaaa
     8461 atgtagagaa catctctgtc actggtttcg gtacattaga tggaaatgga aaaaaatggt
     8521 ggcatacttt tagaaatgaa ccagataatc tcgcttatcc tcgtcctaaa ttgatgagtt
     8581 tccataactg tcatcgaatt actgtgaaag atattaaatt gatccagtca ccaagttgga
     8641 cgatcaaccc gattctttgc agtaatgcaa cgtttgataa cttgacgatc ttgaacccag
     8701 cggattcacc aaatacagac ggaattgatc cagaatcatg caaaaatgtg cgtattagta
     8761 attgccatat tgacgtggga gatgactgta ttgcaatcaa ggcagggact gaagatacgt
     8821 acgaaaggat cgcttgcgaa aatatcacga tcacaaattg cacgatggtc catggacatg
     8881 gcggtgtcgt attaggaagt gaaatgagtg gtagtattcg taatattacc atttcaaatt
     8941 gtatcttcca agaaactgat cgaggaatac gattgaaatc tagacgaggt cgaggcggga
     9001 tcgttgaaga tattcgagta tccaatatag tcatggataa tgtgatgtgt ccatttatac
     9061 tgaatcttta ttatttctgt ggtccgcgag ggaaagaacc gtatgtatgg gaaaaaaagg
     9121 cttatccgat agatgaacgg acatcagcat tcagaaggat ccatttttcg aatatcacgg
     9181 caaggaatgt ccatgcttct gcaggtttta tttatggatt agcggaacaa ttcatacaag
     9241 aaatcacatt tgacaatatc gatgtctcga tggcccaaga tgcagttccc ggtgttcctg
     9301 ctatgatgac aggaatagag gaaatgagca gacaaggatt ttatattggc tttgcgggta
     9361 aggtttcctt taaccagatg tcgattgaaa atcacgaggg accagcattt ctattagaaa
     9421 atactaaaga aatcagtatt gagaaattcc aatcaaatca atcagaaaat atggatgaac
     9481 tggttgttcg taaaaacttt gcttatgcag ttgaatgatt ttttaggtgc tccatctagc
     9541 agacataccc ctagtggaaa acgattatac cgagaaaaac aaaacggttt tatgcttgaa
     9601 tactatcaat tgtacttaaa tgaattggaa acagtaccag ccctctatgc ttatccagaa
     9661 aaagaaggac cgtttccgac ggtcctttac ctgcattctc acggcggaga tttttcagtc
     9721 gggaaaagtg aattgatcca tggtgctcct tacttagctt ctccatcttt tgcagaagta
     9781 ctgacaggaa tgggatatgc tgtttggtcg attgatgctt ggggatttga agaacgcgga
     9841 ggtatttctg aaagcgagtt gttcaaacaa tttttattaa caggaaaaac gttatgggga
     9901 atgcgtattt atgatgtgat cagcttgatc gattatttgg agacaaggga agacacagat
     9961 actcaacggc tcgcaacgat tggtctttct atgggaggct tattaagctg gtgggtgaca
    10021 gcattggatt ctcgtgtcaa agtttgtatt gatctagcgg cacaggtgga catcgaaaca
    10081 cttttgctac atcgccgcct agatcatcat gggttttatt actatgtgcc aaatctattg
    10141 acagcttata caacgttagc aatccaagaa aaaatcgctc cgcgccctcg tcttagctta
    10201 gttggaaaaa acgatacgat gtgcttggac gaaggcgtac tgaagctgag aaaagggctg
    10261 gacaaaaagt atcaatcaat gggtagtcca gaaaactttt cttctttttc tttaacaggc
    10321 ggtcatatgg aaacacaaga aatgagaaat atttggaaac aattcataag ggagcattta
    10381 tgaggatgaa agacaaactg ataattggaa agaatgctat agataccccg acggattttg
    10441 aaactatcca agaaggaatc gattatttgg caggcctgcc ggatagccat ccaaaggtct
    10501 tgatagtaaa agaaggaaga tatgatgaat atatagaaag cagattatcc aattttaaac
    10561 ttgtgggagc aggaacagta gttatctccg gtaatcgttt tgcaaaacag gagtatcttg
    10621 acggtagcca gttagggact ttccgaagtg ctactttttt cttagaagga tcgaatgtcg
    10681 tattagaaaa tcttcacatc gaaaatacag caggtccagg agaaaaggta ggacaggctg
    10741 ttgctttatt caattcagga gatcgcgtat ctttaaaaaa ctgtcgacta tctggttatc
    10801 aggatacctt atgcacgggt ccgttgcctg atttgcaaaa agatggtact cctttttgta
    10861 caccggcttc tgccaatcct gagtattgtc gtcagaccta tgaatattgc tggattgaag
    10921 gaacggtgga tttcatattt ggtggggcag atgccgtttt tcatcagtgt catatccata
    10981 gcttagccag taaaaatcct ggatacatca cagcagcatc tacgccgaaa aatcaaaaaa
    11041 cagggtatcg ttttgaaagc tgtaccttga ctgcagaacc tgatactaga aacgtctatt
    11101 taggaagacc gtggcgtccg tatgcaaatg tcttatttga agcgtgtaag ataggtgggc
    11161 atgttcaccc aactggttgg gataattggg atgacaagaa aaatgaacag actgtcaaat
    11221 tctctgaacg taataacaat tatgaaacat cgatcaaaag acctgaatgg atacatttag
    11281 aaaagcaggg ggttcagaaa tgaaaagaaa atgggaacta ttgctaggaa tggtcggtgg
    11341 cagtctatct ttgattttct ttggcgggtt agcagttact ttaagtaata tgtctgcgtc
    11401 agagtttaaa aaaagttatc agtcgcttgc tgtagatcat tcaactttgt ctttagaaaa
    11461 tacatttgga ttattacagg acatgactgg tctgtttgcc gttgtgttgt ttatttcact
    11521 tgctttttta gcagctgggg cattcagttt atatcgagta agaatgagaa aaggagcctg
    11581 aatcatgcag actatcaaag tgaaaaatcc tattttaaga ggattcaatc cagatcctaa
    11641 tatattaaga gtgaaagagc ggtattacat tattgtctcc tcttttgaat ggcttccagg
    11701 aatccgagta tatgagtcga aaaatttagt caactggtca catacaacgg atattttgac
    11761 cactcaagta tctttgcaag gaaatcctac aaatggaagt atatgggcgc cacagatcag
    11821 ttatgctgat ggattgtttt atcttgttta cacagatgtc aaaagtgcag gccgtccgtt
    11881 taaagatgtc cacaattatt tgatcactgc atctgagatc acaggtcctt ggtccgaacc
    11941 aatttattta aatagtaacg gctttgatcc ttctttgttc catgatcttg aaacgggaaa
    12001 aaaatggctt gtcaatgaac tatgggatta tcgtctggac acaccgaata aatccagcgg
    12061 tatcgtatta caagaatatg atgcagagaa gaagaagctt gatggtccca tctataaaat
    12121 atttgcaggt acagaattag cgaaaacgga ggcaccacat ttgtattttc gtaacgggta
    12181 ttattatttg atgacggcag aaggcggtac aggaagagga cattcagtta cagtttgccg
    12241 agcaaagaat attactggtc cttacgagct tgatcctggt tttcctatgc tgacagctag
    12301 tgacaaacct gcttctacat tgcaatgtac aggacatggg agcttagtac aaaccccgtc
    12361 aggaagatgg tacatgacat atctttgtac cagaccatta aacggtgcgg ctattttggg
    12421 aagagaaacc gctattcaag aagtgtattg gacgtccgac gattggttaa gacttaaaga
    12481 tagccagaca actccgatgg aagaaataat gattgagaca aatgaagagg tacaacaaga
    12541 aaaagatcat cagtttatgg atacatttga aggagagcta aagaagaact ggaatgccag
    12601 acgcattttg ccaaatgctg attggtgtga tacaaaatgt agaccaggtt ttttacgtct
    12661 gatttccggt gaatctcttc aatctacctt cgaacatcat ttgttagcta ttcggcaaac
    12721 ggattttact tttgatgcgg agacagcatt tgaatacatg cctaataatt ttaatcagat
    12781 ggctggactg gtactgtact taaatgaatc caattatatt tattgttatg ttacttggaa
    12841 tgaggacaag ggaaaatgcc tacgtatgat agaatcagaa aacggagtag cacaaataga
    12901 agattggacg attccactca cagaaaagaa gacttttttg aaagtcactg taaagaaaga
    12961 gacagcacag tttcatttga aagacgtatc aactgaagaa tggaagaaag taggtccgca
    13021 aaaaaacatg tgtatactct caggagggtt tactggaaat tttatcggga tagcagtgca
    13081 tgatctaaat aaaaaaagag gaagttatgc t
//
LOCUS       DAZUMB010000073        13026 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000073 DAZUMB010000000
VERSION     DAZUMB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.074"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..1531
                     /locus_tag="ACG80L_001817"
     CDS             230..1531
                     /locus_tag="ACG80L_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5024043.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1558..1818
                     /locus_tag="ACG80L_001818"
     CDS             1558..1818
                     /locus_tag="ACG80L_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5024044.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2817..4052)
                     /locus_tag="ACG80L_001819"
     CDS             complement(2817..4052)
                     /locus_tag="ACG80L_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5024045.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            4620..5447
                     /locus_tag="ACG80L_001820"
     CDS             4620..5447
                     /locus_tag="ACG80L_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024046.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(5720..6490)
                     /locus_tag="ACG80L_001821"
     CDS             complement(5720..6490)
                     /locus_tag="ACG80L_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5024047.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            6708..7775
                     /gene="ulaG"
                     /locus_tag="ACG80L_001822"
     CDS             6708..7775
                     /gene="ulaG"
                     /locus_tag="ACG80L_001822"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS5024048.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7793..8254
                     /locus_tag="ACG80L_001823"
     CDS             7793..8254
                     /locus_tag="ACG80L_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5024049.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            8274..9758
                     /locus_tag="ACG80L_001824"
     CDS             8274..9758
                     /locus_tag="ACG80L_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5024050.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            9801..10100
                     /locus_tag="ACG80L_001825"
     CDS             9801..10100
                     /locus_tag="ACG80L_001825"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5024051.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            10115..10753
                     /locus_tag="ACG80L_001826"
     CDS             10115..10753
                     /locus_tag="ACG80L_001826"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS5024052.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            10758..11618
                     /locus_tag="ACG80L_001827"
     CDS             10758..11618
                     /locus_tag="ACG80L_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS5024053.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            11608..12318
                     /locus_tag="ACG80L_001828"
     CDS             11608..12318
                     /locus_tag="ACG80L_001828"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5024054.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            12550..12984
                     /locus_tag="ACG80L_001829"
     CDS             12550..12984
                     /locus_tag="ACG80L_001829"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5024055.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         4134 a         2031 c         2731 g         4130 t
ORIGIN      
        1 atgatatgtt tcaatcacta gatttaatca ttttgatttt tgttaattat cgaatttttt
       61 tgttgctcct atgatttaga aaacttattt tcaggattct taaaaataaa tttgattatt
      121 taagagaata tattgaaata aaatttctta attatataat tagttatgaa cattctttgc
      181 gttgtcatgc aatgaatggt tcatatttta ttgaaaagga gtggttttag tggaaaagct
      241 gatgaaatgg gtcgaagaga aacttgtccc accaatggca aaaattggta cacagcgtca
      301 tttattagca attcgtaatg gggttgtttc aacattgtca ttgattttga ttgggacatt
      361 ttttatggtc tttatcaatt taccgtttcc tggatggaat gaatttattg ccccttattc
      421 ggcaacgatt gttttaccct ttaggatgac gatgggattg atggctattt atgcaacttt
      481 tgttatgggt tcggatctag ctaaatcata tggattagat agtgtgacag gtggtatatt
      541 atctttagga acgttcttta tgttacaagt tcctgtaaac gttttaactc ctgaagaggc
      601 acctcttggc tgggtcttac ctatgagttc tttgggagca tctgggatgt ttgcggggat
      661 cttatctatg atttttgcag tggaagtata tcgtttcttt aaaaatcgaa atattactat
      721 caaaatgcca gaacaagtgc cacctgctgt tgcccgtagt tttgaggcat tgattccagg
      781 tgcagtcatt ttaacaatta cttggctgat tcgaagcgtc gcgggctttg atgtaaacgc
      841 tgccttatta tcgttattcg agcctttaac caatatttta gggaataatt tgcttggtgt
      901 tttactacca atgtttttga tccatttgtt gtggtcattt ggtatccatg gcatgagtat
      961 tgtaggggct gttgttcgtc caatgtggct gatcatgttg gatgagaatg caaaggcatt
     1021 ggcagatggt acagctgcca cccaattacc ctatatcgct cctgaacagt tttatcaatg
     1081 gacggtaaca attggtggtg caggagcaac gatcgtggta tctgttttgt ttatattctt
     1141 ctgtaaatca aaattcttga aagaggttgg tcgtttttct attattcctg gtatcttcaa
     1201 tatcaatgag cccataattt ttggagctcc tatgatgttg aatccttaca tgttcatccc
     1261 gttcaactta gttcctttag ttttgactat tatcagttat tttgcagtca aattagaaat
     1321 ggtcaataga tttactgttt tgccaacatg gacattacca gctccaatcg gcggttatct
     1381 ttcagcagga aatgactggc gtgttgttat cttgatcatg atcaacacat tgatagcatt
     1441 tattatctat tatccatttg taaaagctta tgataaaaag atgctgtcag atgaacaaag
     1501 tcgtggagaa aacgaagtcc agactgtcta agtttggttt ttagaaggtg ggaaacaatg
     1561 tattggctga ttttattttt tgtttttatc tttttattaa cggctagtca ccttatattg
     1621 aatatgctag ctacatatca tattcaaatc aatcgatgga tttgggcact ggcatcattt
     1681 ttgatcgtga ttcttccaaa gatcattgta ccgcatatga atgttttgtt ttcatggggg
     1741 acatatgtac tttgtggaat ttttgctatc aattttatga tcgagcagca tagatggttt
     1801 gtcacatcga agctttaaat gttctaaaag tggttgtaac agaagtggtt agcttcaaga
     1861 aataagaaga aatttacgaa aattgcttct taaatttttg tgaaatgttg gcttattttt
     1921 cgtttggttg aacttattac cgcagatgca agcttttgac caccgtttat tcggaatatg
     1981 ggactgtgac aaggcttttg tcacagccct tttttacata aagattatta ttgttttaac
     2041 tgttttggca gaaagaagga ggaaaaaatg gaaattttat cagcaatgga tttagaatta
     2101 gtagatctac taccaaagtg atcaagtcaa atatagattt gggagcttac aaccatatag
     2161 taggggctgc agtatggtgt atgaatggta atatgtatga tgtgagtgtt tattcaaatc
     2221 atgaaacgtg tatagaaata attgtgctgg gaatagcaat cagtcatggt gaacgtgact
     2281 tttagtgtat cgttgctgac gacatagaac attctgacat gatctactca atatgtgggg
     2341 attgctggca gtttatatag gagtatgcat cggattgcga gatcattgtc tcaacagaag
     2401 agggaggttg attatcaaag ataaagtggt aaatcctaca tttgatttag taattgttga
     2461 ggggactagc ggcacaaatc tctacaaagc gatgcttcta gatctatatg acacatttta
     2521 agaaaatgta tttgcctaat tattatggtc tttcttttga agaaacgttg agatgatcca
     2581 accagagaaa ggtgtcaaag aatttgacta agaataaatg gcagacgcta cctatgctca
     2641 tttggatagc gtcagatctt ttattttaag gatgattttg tgttttttag acaccgtaat
     2701 ttaagtaact ctaacttttc aatccactat ttactttttt tgtattttca ctgtctgggt
     2761 ctcgaatagc gaaattactg atgagtctct attaaaaaag ctttttctac ttgaaactat
     2821 attgggtggt tgctcaggaa ggtttcgatt tcttgaattg cttgttgtgg accatttatt
     2881 ggaaaaacgg cttgcagcgt gcgtgcctgc tgacgaatct tgggtgaaga aacttcgcgc
     2941 agagcaatct gcagttttct ttcagtcact ggttttgtga gggcgattgc gtttccgtat
     3001 tttacacaat agtcaatatt acagctttgc tcaaattgca tccccacacc gataaacggt
     3061 ttgccggatt cacaggcagt ttggacggtc ccttccccgc cgtggataaa agaaaaatca
     3121 atgaattcat gcaaaagatg actagacaga tattctgtta ttaaaagatc ttctgagtca
     3181 gattgaatgt catcaagcag atagtcaata ggtgcaataa ccgctagatc cggttgtttt
     3241 tgtaagaaga acaaaatttt gcgtaacata cgcggattgc ctgaactacc cattgcgaaa
     3301 tagataatac gttttctttc ttgttgcttt cgctgtataa aagttagcac ttcttcaggc
     3361 aaggaagtac tgagcttagc aaaaattgga ccaacgattc gataattttc cggcagctgt
     3421 tcttcttcca aaaatagttt aggcgttgtg atgagattta gatcagcatc catcatttgt
     3481 aaacttgttt ttggtaaagg aagcatttct ttttgtgcag cgtgtttgaa ggcattgggg
     3541 atccatcggg tatttactat acgctttttt gtccagtgcc aaaggggatt gcttaaaggg
     3601 ccgaagaaac gtaactcgtg gggcatcagc gcatgctctg attgaaaaaa aggttcactc
     3661 aaagcatagg gtttaacata tactaaagga atctttttat tgcgggccga taaaaaaatt
     3721 gtcacattcg aacctgtcac aatcagtcga ggctcaaact tagcaatcag actctgttca
     3781 ctagtgatac gagaggcaac catttctttg gtaaaagggt ttttaagtgt acgcaactgg
     3841 tcaaatttta tgatttgttc ttcttgtttt ttcgtaagga tcggcgctag taaatgatac
     3901 tcaaagccac tttctgtgat caaaggagca aaagttttag aaaagccaaa gaatacacag
     3961 taataacggt ctttcatagc tttggcaacc tcaatcattc gtgttgtctc agctaaattg
     4021 aatactgcag gagcaaaaag aattgtcttc atgaaatcct ccttattgga atgagtctat
     4081 cccttcgttt cttactagta tactaaaaaa cacagcgttc gagtagccta tgtatatttt
     4141 gaatattaaa ctgtctctcg ctgttttcac aacttctaaa tttaaatttt gtaatcaagt
     4201 tgaaactcca attttcaaaa aaacacgcaa caattttttg atcgatttat atgttgtgcc
     4261 agataaatac gagaagttgt gtcattaaaa ataaacatga gaacataaaa aaataaaata
     4321 attatattaa ttgataggtg tgctgtcgtc attatacaca cgtaagaaag aaatagttat
     4381 gtttaaaaat attcttagac tttctcgcca tgacaatttt cactatgtgt tataactgta
     4441 aacgataatt attcagataa cacaaaaaga aatcaaggag cagatacgcc agttgtttaa
     4501 tttgaatatt attttaaaat taaaattttt attctgttct aaacattttt ggttgtttag
     4561 aacttttttt tgtgtatgat ataaaggaga aataattttt taaagggaag gaaattgttt
     4621 tgattaaagt agatggagca ttactaaaag aataccgaaa gaaaaagggg ttaacacaag
     4681 tacaattagc agaaggcatt tgtgaacaag caactgtcag caatatcgaa aacaagaata
     4741 tgtcgccagg catcaatatc ttaagtgcgc tttgcaaaaa gctgaatgtc gagatctccg
     4801 atatattagt agaaacagaa gaagacaaaa tcaggaaaaa aataaatgag attcaatcat
     4861 tgaattctgt atcaaaatac caagaatctt ataaaatttt acaaacgatc gatcaggaaa
     4921 aagtaagtaa ttctgatctt ttaaatcaaa ttttatttta tcaaggactg cttgatcatc
     4981 tttatgcgaa aaaaccagat gcagcattat tttattttaa tcaagtgttg gaacaaacga
     5041 ccgaaacaga tatccatcac ttacaagcag ctgcaaacgt ggcaatgatc tacttgtcaa
     5101 aaggagaatt agattttgcg aaagtttatg tggagcgtac acttaaaata ttgagtgaga
     5161 ctgattttga taatctgatg gtctgcattg tatattatga tatcgccacc tattaccgta
     5221 aaatcgagga ttatgataaa gcaatccaat tatgtgagaa gggtattgag tataataaga
     5281 aacataaatc gacttatgca ttggaatact tgctttatga aattgcttct tgccataagc
     5341 aattaggaga agatgattat ttagaaagat atatggacgc aaaaaaaata gcgcgtttca
     5401 acggaaatga ttacgcggta aaggtgattg aaaacgacct gaaataaagt ggttgtgaaa
     5461 tcagtgttct gatttgaata ccgtttattc gaaaaaagga ggtgtgactt ttgtcatacc
     5521 tccttttttc gatattgttt gattcctatt taggcaacat ataacggtta taaaagaagc
     5581 aatagattat gatggtatcg attggatgga tacatcttgg gattccttta tcaatttgag
     5641 cgtagcgtgt ttttcattta tcgataaatt tgttattcat ttttttgata atcctattta
     5701 catatttacg tcctggttct taaaatgaat ctacttgaat ggtttttatc cccgtatttc
     5761 tgatctcctc taatactttt tcagaacaaa atttatcagt gatcaaataa tcaaagactg
     5821 aaatatcagc aatcttgaac tttgatgtca gtccgatttt tcgatgatca gcgactaaaa
     5881 tcttacattt gattgtcttg ttgaccatca tttcgttgat cgttgattca tttaatatct
     5941 ttgttgtgac cccatgtact acatcgactc cgctacagcc aatgatacaa atgtctgcat
     6001 tgactgaaga aatcgtattt aatgcgatgt cgcccactag tgcttctttt ggaaagcgca
     6061 tctctccacc agttaaaata taactacaat tatctttgtg cggagagtaa ttgattttca
     6121 cgttgtttgt aatgactgtc aaatggttag attgaagaaa atccagtgtt ttcaacgcag
     6181 tcgagctaga attgataaac agcgtatcat aatcgttaat gtatttacta gcttctctgg
     6241 caatcgcttg tttaatcctt tcaggaccag attgatcaaa atcagtctgg tttttgtaat
     6301 tataagaaca ataaccgtaa ttgcgtatga tctcgttttt ttcttctaat aaatttaaat
     6361 ctcttcgtat cgtactaata gatacatcaa gggattcagc taattcgaaa gttgatagtt
     6421 gttcagtctg ctgaagcagt tccaaaattt ctttttgacg tttttcaata ttgacaagtg
     6481 agtttttcat gacttttgcc tcctgaggga catcttgatc catggattta tttgactagt
     6541 tgttcattta aacattcata tctgtcttta ttatactgaa acaatcattg attactgaaa
     6601 gaaaatcata taaaaaacaa acggtcatat aaaagggttt tcatggatgc tgatacacat
     6661 taatatttac ttgtaagttg gaacaacgat atggggggag caagcaaatg agtaaaactg
     6721 tcaatgaagt aacaaaagaa aagtggttag tagaaacatt tcctgagtgg ggcacatact
     6781 tgaatgaaga aatcgaaaca gaagtagtgg atcctggaac ttttgctatg tggtggctgg
     6841 gctgtacggg aatttggctg aaaagtcatg aaggaacgaa tattttatgt gatctatggt
     6901 gtgggacggg aaaacgtagt catgggaatg gcatgatgaa aaaaggccat cagatgatgc
     6961 gtatgagtgg cgttcaaaat atgcagccta atttacgtac tcagcctttc gtaatcgatc
     7021 cttttgcgat aaaaaatgta gacgctttaa tcgttacaca tatccattcc gatcatctag
     7081 atatccatac agccgcagca gttcatcaaa attgtcctga agcaacattc attgggccaa
     7141 aagaagtggt tcgcacgtgg caaaaatggg gaattcctga agaaaaaacg attacagtaa
     7201 agccaggtga tgcagtcaaa atcaaagaca tcaatgtagt agctttagaa gcgtttgatc
     7261 ggacagcgtt ggtcacttgt gaagatccag aagaggtatt aaaaggaaaa atgcctcagg
     7321 agatggatca aattgcagta aactatttat tcgaaacatc aggagggaat ctttatcatg
     7381 caggagattc acattactcc aatttatttg ccaaacatgg taatgaacac aaaatcgatg
     7441 tctgtttagg ggcctatggt gaaaacccac gagggataac agacaaagtt acttctgttg
     7501 atatgctgcg catggcggag tcattgaatg caaaagtagt gattcctgtc cactacgata
     7561 tttggtctaa ttttatggct gatccaaaag agatcactga aatttggaaa ttcaaaaagg
     7621 atcgcctgaa gtatggattt aaaccttata tctggcaagt aggcgggaaa ttcatctatc
     7681 cgacaaacaa agatgatctt gagtttaatt tttatcgagg atttgaagat gtcttctcga
     7741 ttgaaaatga tacaccgttt ccgtcgtttt tataagaaag aaggggagtt tcttgctgaa
     7801 atactttttt gaccatcaat tgattcatta cccagataaa gaaatcacgg attggcgtga
     7861 agctatcgcc gaaagctgtc ttgtattgat cgataaggaa attattgatg aaacgtatgt
     7921 ggaggagatc atccattgtg tagaaaaata cggtccttat attgtgatcg cacctcaagt
     7981 agcaatgccg cattcttcag aaaaaagtga tggtgttttt ggaacagcta tttcatttac
     8041 caagttgaaa cagccaatcc gttttgaaag tgaaggggag aatgaggaga aaacggctag
     8101 tttgtttttt acactcgcag caaaagatcc agaagaacat ctagaaaata tccaaaaatt
     8161 atccgaatta ttaatgacag atggattggt tgagcgtctc atctctacga actccatcga
     8221 tgattaccaa gttgtgatgg cagaatataa ttaattgata gaggagaaaa aagatggggg
     8281 aatttttgtt aagcatttgg acgtattttg caacaaatat cttgacgcag ccagcatacc
     8341 tgattggttt tatcgtgtta ttaggctata ttttgctgaa acgcccgctt catgaatgca
     8401 tcgctggatt cttaaaggca acggtcggtt actttatttt gactgttggc tctggtggtt
     8461 tggtcaataa ttttcggcca atcttggttg gactaaagga acggttcaat ttggatgcca
     8521 tggttacaga tccttatttt ggacaaaatg ctgtggatgc tggattaatg gagacatttg
     8581 gccgtacctt tggagatgtg atgattttat tactgattgc atttgtttta aatatcttat
     8641 tagtccgttt tcagaaatat acgaaactgc gttccgtttt cactaccgga aacgttcagg
     8701 ttcaacaagc agctaccgct ttttggattt tactgttttg ctttcctgat ctaggtagga
     8761 tacaagcatt gatttttatg ggactgattt taggttgtta ttggacagta gcttctaact
     8821 taacagtagg aatcacgcag gaattgacag aaggtgccgg atttgcagtg gcacatcagc
     8881 aaatgttcgg aatctttata tttgcaaaat tagctgagtg gatgaaaaag cgggatgaaa
     8941 agaaaaatag atcaatcaag caagacaaaa aaattgaaga tatcaaatta ccaggattct
     9001 tgtcgatttt caatgagaat atggttgcta cttcgttact catgctattt ttctttggga
     9061 tcattctgat tgtactaggc aaagattatt tgattcaagc gcaattcatg caagaagggc
     9121 agagtttctt attctacatt atgacgactt ctttaaattt tgcggtatat ctggcgatct
     9181 tgcaattagg tgttcggaca ttcgtcgatg aactaactca atctttccaa ggaatctcaa
     9241 acacgatttt accaggagct gttccaggaa ttgacgtagc tgctactttt ggctttggtt
     9301 cgccaaatgc ggtaacgatt gggttcttgt ttggtgcttt gggacagttt ttgatgattg
     9361 gtttactgat tttattgaaa tcaccaacga tcgtcatagc cggtttcatt ccgttgttct
     9421 ttgataatgc cgttatagca gtttatgcca ataaccgagg tggattcaaa gccgcttgcc
     9481 tgttcccgtt tctttcagga atcatacaag taggcggttc agcattattc gctacatgga
     9541 ttgggatgtc aacatacggt ggttatatcg ggatgttcga ctgggcaaca gcttggccat
     9601 tgtttacagt aatcatgaaa gtcctaggtt atgctggaat tgccgtagtt gtcattggtt
     9661 tattggctat tccgcagtta caatatcgga aaaatcctga tggttatttt ttgattgtag
     9721 aagactatga cacatatgca gaaaagcaga gagtataaaa gaaaatctga ttagacaaca
     9781 ctcataatgg agggattaag atgagaattt tagtatcgtg tgcaaatgga tcaggaacaa
     9841 gcttgatgat gatgagaagt gtggaaaaag caatgaaaga actaaacgta ccaattacca
     9901 aaatccatca ttgtgccatt tcagagggaa aaagttcagc tggccaatac gatgtagttt
     9961 ttacgccagt caattttttg aatatgttta ctcaggcaga gaaaaaagga gttacagtta
    10021 tcggtatcaa aaatgtgatg tcagtcaaag aaatcagtga aaaatttgct gaaacaaagt
    10081 ggtaccaaaa aaacaagtaa aggaaggaac taagatgagc aggccgaatt tgcaaattgc
    10141 gttagatcac aatgacttag aacatgcatt aggagatgtt atgaaagttg gtgatgtagt
    10201 agatattatt gaagtgggca caattctttg cttacaggaa ggacaaaaag cgattcgctg
    10261 tattcgcagt atgttcccag ataaaaaact agtcgctgat acgaaatgtg cagacgctgg
    10321 aggaacagtg gcgagtaatg ttgcaaaagc aggagcagac tggatgacag tcatttgttg
    10381 tgcaacgatt ccaactatgg aggcagcaca aaaagaaata ggtgaattgc aagtagaact
    10441 ctacggcaat tggacttttg aacaagccat ggattggcat aacatcggta tccggcaggt
    10501 aatctaccat caaagccgtg atgcgctgtt atctggtgaa acttggggtg aaaaagattt
    10561 atcgaagatc aaaaaactga ttgaactagg ctttaatgtt tctgtaactg gtggattaaa
    10621 tccacacaca ttacacttat ttgaagggat cgatgtgtat acctttatca ctggtcgtgg
    10681 tattacagca gcaaatgatc cgatgaaggc cgcacaaaac ttcaaagatg agatcattcg
    10741 tatctggggg taaggaaatg gctcaaattg ggatttatga aaaagcgtta cccaaagata
    10801 tttcatggaa agaacgtttt tcccttgtaa aagaaatgga cttcgatttt atcgagatgt
    10861 cgattgatga gacggatgag cggttagcac gattagattg gtctgaagaa aagatagctg
    10921 agctgagaga agagatgttc agctccggtg tacgtatcca ttcgatttgt ttaagtgcgc
    10981 atcggcgatt tccgtttggt tctgctgatc cagaaaagaa aaaagcagcg aaacaactaa
    11041 tgaaaaaagc aatccatttg gcgcataatc taagcgtcaa agtaatccaa atagctggat
    11101 acgatgttta ttatgaagag aaatcaatga attccagaga agactttatc gaaggaataa
    11161 aagaaagcgt aaaggaagct tctaaatatg gcatcattct ttccgtggaa atcatggatg
    11221 atccatttat gaactccatt tcaaaatttt tagaaatcaa agaacagatt cattcacctt
    11281 ttctacaagt atatccagac ctaggcaatt tgtccgcatg gccggaaaat aatccggctc
    11341 gagaacttga gaaagggatc gattatatca ctgctatcca cttaaaagat acttaccctg
    11401 taacaaaaga atattcaggc cagtttcgtg atgttccttt tggtagcggt tgtgttgatt
    11461 ttcttggatt attaagacat ttgaaacgtc tagattatga tggaacattt ctgattgaaa
    11521 tgtggagtga aaaaagccaa gactttcgaa gagaaatcca gcaggcaaaa gactacttaa
    11581 gttctaaatt gaaggaggct ggctatgatg tcttgtgaag aaaaaattgc agcaatgaag
    11641 caacgtgtcc ttgaagcaaa tctggcattg ccgcgatacg gattagtcaa attgacttgg
    11701 ggcaatgtga gtgaagtgga ccgtgagcta ggagtcatcg tcattaagcc aagcggtgtc
    11761 agatatgaat gcatgcaagc agatcaaatg gtggtaacag atttgtcggg aaatataata
    11821 gaagaagata gcttaaaacc atcttcagat ctccccacac atgttgtttt gtatcaaact
    11881 tttgaagata taactgctat tactcatact cattccacgc actccgtcat gtgggcgcaa
    11941 gcaggacgcg atttgccagc ttatggcacg actcatgctg atgcttttta tggtaaggtt
    12001 ccttgtacac gccaattaac gaaagaagaa gtaagagaag cttacgaagt tcatacaggg
    12061 aatgtgattg tcgagacgtt taaggaaaga aaattagatc caaacgaagt ccctggagta
    12121 cttgtctatg gacatggacc gttcacatgg ggggattcac ctatgaaagc agtagaaaac
    12181 agtttgatct tagatgaaat ttgtctgatg gccaaagaaa acgagctgat caaccccaat
    12241 atatgtgaaa tcccgcaata tttactagat aaacattatt ttcgcaaaca tggaaagtat
    12301 gcatactacg ggcaatagag atgctagggt aagggtagaa aattaaaaga taacacttga
    12361 tagagagaaa cagaggttgt gaagaaaatt tatcacaacc tttttattta tcaaaaagta
    12421 tgcattctaa aaggaaagag gaattttaag gaatcgagag aatctttaca ggagagttat
    12481 atgtgataga ttaaatagtc aaagaactgt attattagag ggagaattgt ataagaaagg
    12541 aagaaaaaaa tgaatattga ttcccttatg atcgatgaga tttctgaatt atataagagc
    12601 gttggttgga cgaattacac aaaagacact gcaaggttag ctagagcatt tgaacaatct
    12661 gaatctctga taaagcgtaa tggggaaggg aagattatcg gaatcgttcg ttggatcact
    12721 gattttgcga cgatcgcatt tattcaagat atcctcattc atccgaggta tcagcgccaa
    12781 ggaataggca aagtgctttt gaatgaagct ttggagaaga tcactagtta tggaccagtc
    12841 caaatcgaat tattgacaga tgatactgag aaaacaaaaa aattttatga atcagtagga
    12901 tttgttcaag taaaggaaat gggtggtatt tcttatgtca tagatactag aagaaaaaag
    12961 gtgctttttg gtaatgacaa ataacatata ataacgtaca aaagaaaaaa ttctagctta
    13021 gttctt
//
LOCUS       DAZUMB010000074        13019 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000074 DAZUMB010000000
VERSION     DAZUMB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.075"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..327)
                     /locus_tag="ACG80L_001830"
     CDS             complement(<1..327)
                     /locus_tag="ACG80L_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS5024056.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            504..1232
                     /gene="sfsA"
                     /locus_tag="ACG80L_001831"
     CDS             504..1232
                     /gene="sfsA"
                     /locus_tag="ACG80L_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS5024057.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1248..2261
                     /locus_tag="ACG80L_001832"
     CDS             1248..2261
                     /locus_tag="ACG80L_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5024058.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2261..3538
                     /locus_tag="ACG80L_001833"
     CDS             2261..3538
                     /locus_tag="ACG80L_001833"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5024059.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3601..6303
                     /locus_tag="ACG80L_001834"
     CDS             3601..6303
                     /locus_tag="ACG80L_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5024060.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6455..6772
                     /locus_tag="ACG80L_001835"
     CDS             6455..6772
                     /locus_tag="ACG80L_001835"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5024061.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6802..7122
                     /locus_tag="ACG80L_001836"
     CDS             6802..7122
                     /locus_tag="ACG80L_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5024062.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7230..8690
                     /locus_tag="ACG80L_001837"
     CDS             7230..8690
                     /locus_tag="ACG80L_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5024063.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8758..8979
                     /locus_tag="ACG80L_001838"
     CDS             8758..8979
                     /locus_tag="ACG80L_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024064.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9010..9192
                     /locus_tag="ACG80L_001839"
     CDS             9010..9192
                     /locus_tag="ACG80L_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS5024065.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9192..9605
                     /locus_tag="ACG80L_001840"
     CDS             9192..9605
                     /locus_tag="ACG80L_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS5024066.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9728..10909
                     /locus_tag="ACG80L_001841"
     CDS             9728..10909
                     /locus_tag="ACG80L_001841"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5024067.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10980..11144)
                     /locus_tag="ACG80L_001842"
     CDS             complement(10980..11144)
                     /locus_tag="ACG80L_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024068.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11298..11371
                     /locus_tag="ACG80L_001843"
     tRNA            11298..11371
                     /locus_tag="ACG80L_001843"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11332..11334,aa:Arg,seq:cct)
     gene            complement(11440..12579)
                     /locus_tag="ACG80L_001844"
     CDS             complement(11440..12579)
                     /locus_tag="ACG80L_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5024069.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(12689..>13019)
                     /locus_tag="ACG80L_001845"
     CDS             complement(12689..>13019)
                     /locus_tag="ACG80L_001845"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS5024070.1"
                     /translation="IENFPEKYYRTTISYKLKNKATSPFSLSSYQASLIDGDGMEYSR
                     TAGEYFLYDENSNSLVQPETTTSGVFYLLSKNKPNLSTFKLNASKQFTDDFTAEPIGE
                     AGTTEYK"
BASE COUNT         4213 a         2197 c         2661 g         3948 t
ORIGIN      
        1 tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag tttgttcggg
       61 ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat attcatctat
      121 ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat cagatgtaat
      181 ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat actttgagag
      241 tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg agagaacggt
      301 aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc tttatgagaa
      361 aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa aagctaaaat
      421 ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga taggatacat
      481 agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct ttttttattg
      541 accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata attattgcgc
      601 atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa gtggctattg
      661 tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta aaaaagaagg
      721 gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat ggtattttag
      781 acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag cgtgaagtaa
      841 cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa aaagcatttg
      901 ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct gatgcgccca
      961 cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat ggttatgccg
     1021 cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc catgaagaaa
     1081 tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt caaatcctcg
     1141 cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt ccatttgatg
     1201 tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg atcaacttag
     1261 gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta agcacaaaaa
     1321 aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa tttggttctc
     1381 attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat attactcata
     1441 tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct aagcaagcaa
     1501 ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca aaagaaatgg
     1561 atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca gcaagaaata
     1621 tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac gagataatcg
     1681 gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta gatggagaag
     1741 aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta ggtgtttacc
     1801 ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat ttcccaagaa
     1861 agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag aattttgatg
     1921 tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt tattttgact
     1981 caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt catgatcgcg
     2041 cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg gcagatgaag
     2101 cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga ttgttgtatg
     2161 aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta agaaaaaatg
     2221 gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat ttgtacaagt
     2281 atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac cttctcttat
     2341 tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg tttctcctac
     2401 aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg aaccaggtga
     2461 agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag tagcagatgt
     2521 tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg gcggagccaa
     2581 cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg gtacaccagg
     2641 aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga aaacaatggt
     2701 atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg atcaaattct
     2761 tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag atcgttccct
     2821 tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga cagcagagga
     2881 cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa agaaagatga
     2941 ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct ttaatcaact
     3001 gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg taggaagtct
     3061 ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca aaaagaaaat
     3121 ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta caggtcttcc
     3181 ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc gttcgggaag
     3241 aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata ataatctaag
     3301 agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt ttttatacgg
     3361 aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag taaataaaaa
     3421 gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag aaaaaataag
     3481 aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc
     3541 gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag gaggggttat
     3601 gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca aggattgaca
     3661 gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg atatttaagt
     3721 gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt ttactctttg
     3781 ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca agtgactttt
     3841 gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc aaaagctgcg
     3901 attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg tacagggaag
     3961 tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct ttctgcagat
     4021 gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact gctctttgga
     4081 cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag tcccggtcta
     4141 atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt accgccggaa
     4201 gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct tggagaaagt
     4261 gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc ttcagaaagt
     4321 ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa tcttgcttca
     4381 cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt
     4441 ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg
     4501 gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa atctttttta
     4561 cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact ttcccttcaa
     4621 gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg attcatggac
     4681 agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg gtcacaagat
     4741 ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc tctttccgaa
     4801 accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga tgcttatttc
     4861 cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt gccagatgaa
     4921 atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga ccgtatttat
     4981 aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga tcgtgtgaga
     5041 gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt taagagggaa
     5101 ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt tgagattcct
     5161 tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga aagtgagcct
     5221 gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac tgcttcaagc
     5281 atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt taatatgcca
     5341 ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa
     5401 gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa caccttcagt
     5461 gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac aagtaccatg
     5521 attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga tatttatcaa
     5581 aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa
     5641 actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc
     5701 aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact gatccagatg
     5761 cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga agaatatgag
     5821 atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt tttttcagcc
     5881 tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga tgatgagatc
     5941 ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt ttctattgat
     6001 gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt gtcgattatc
     6061 gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt agacggattg
     6121 atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa aatttatcgt
     6181 ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata tcaggtaaaa
     6241 attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa aataaaaaaa
     6301 tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta taaaaaagag
     6361 ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt cttgcgttat
     6421 ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt
     6481 ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga
     6541 aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata ataatttaga
     6601 atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga aagctcaatt
     6661 tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc aagactatgg
     6721 catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta
     6781 tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat catggggtta
     6841 atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc tgctaaaatc
     6901 ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact tatacaagcg
     6961 catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat gcaagtgaca
     7021 ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac tgatttagca
     7081 aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact tcttttttga
     7141 acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga actctcttta
     7201 tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg atggagaaat
     7261 acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg
     7321 cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa
     7381 ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta gcagcagaca
     7441 aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc tggaacggta
     7501 cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt gcccgagctt
     7561 atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt caaggtattt
     7621 cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat gtcgcagatg
     7681 caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca gttggattat
     7741 ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt atgatcatgg
     7801 gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg atcaaaatgc
     7861 ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca gcgacgatcg
     7921 ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat gggcaactga
     7981 tcattgattt ggtgcaaaaa tatattgcag agccattcct aggattatct caagggttgg
     8041 gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt attcatggac
     8101 caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta atcaatattg
     8161 atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg
     8221 aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca tggtttggcg
     8281 gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa cgtgcagatt
     8341 atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac gaaccagtga
     8401 tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg attgctccag
     8461 tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct ccggtatcgc
     8521 agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca ggagcggatt
     8581 ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt tgggcaccat
     8641 ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa gatgaacgtg
     8701 aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg
     8761 attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga aggatacaat
     8821 gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca
     8881 agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa agcacagcct
     8941 tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta aaaagatagg
     9001 atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt gatcgtgttg
     9061 tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac aggtcttttt
     9121 ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga agcaaaggag
     9181 aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt ttttacgata
     9241 aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa
     9301 aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg gctcgcataa
     9361 aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca acgaagaatg
     9421 aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag gttcattaca
     9481 cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa
     9541 tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc gaagcaacgt
     9601 attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag ctagaaggaa
     9661 tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg taggaagggg
     9721 cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg aaatgtttga
     9781 tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac gttttcgtac
     9841 acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc taacaagtca
     9901 agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa tggccggcat
     9961 tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc agatcatgga
    10021 aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg cgccattctc
    10081 ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca gttatcaact
    10141 aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc gtatggattt
    10201 tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa accaacgagc
    10261 tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag gcaattacca
    10321 gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt atcagcttgc
    10381 aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt tttgcagcct
    10441 tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt catgcggcta
    10501 cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa tgatcacacc
    10561 ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc catttaaaaa
    10621 gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat ggaatgaagg
    10681 aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa
    10741 actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag ggtatcgtaa
    10801 agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg aagaaacgtt
    10861 aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat aaaaaacggg
    10921 tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga aggaaaatgt
    10981 taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt tttagggttt
    11041 tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt tttagatgaa
    11101 tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa ggacctccct
    11161 acattatttc attctacctc tagtttatca tctgttaagg actagtggaa acgttttgcc
    11221 taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa acaagctaca
    11281 attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc tcctaagcga
    11341 taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc ctttgtggta
    11401 caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat ttttatataa
    11461 actatcgaat acagcattga ttttttcttt atcctgatct tcaagctctc gaacaatatg
    11521 aagataagta gacattgtag tttctaaact gctatgtcct aaccgttttg atacgctaag
    11581 tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc catgcaaagt
    11641 aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc tgacggcatt
    11701 tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt ttttaacttc
    11761 aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat caatcgtatg
    11821 tttatcaatt gaaattttcc gatttgaagt ctcgtttttt gtttttcccc attcatttaa
    11881 tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga tatcttccca
    11941 tgttaatcct agaagttcag caaatcgcat tccagtagca ccagcaacta acacgagcat
    12001 aggagaagag tactgtgcag aaaggttttc ttctacgagt ttcattaaag atttaaattg
    12061 atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt tgattactgc
    12121 ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca acgatgctct
    12181 aatatggtta ttaaacttaa taacggtgga ttttacatgg tctttcgcat acttatttaa
    12241 aaagcgttga tatccaactc tatctaaatc agaaatcaat actgctggca tatatttctt
    12301 tatattcatt aacgtatctt cgtattttct ataagtgata ggagatactg ttccttcttt
    12361 gtagagttgc atccaatctt caaagtaatc tgataataat agatttttgc gatccccttt
    12421 aagaccttta ttcaggttat attctaattc gttggcggca tcttttgctt cgccttttgt
    12481 cttgaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt atgaaatttc
    12541 gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcta ctctctttct
    12601 ctgatacaat aggcactgta aagaagccta ttgtgtaggt ttgtttttaa cgcgtccaca
    12661 aactttggcg agggaggggg cgtgtttttt atttatactc tgttgttcct gcttcgccaa
    12721 tcggctcagc tgtaaaatca tcagtaaatt gtttagacgc attaagttta aatgtactta
    12781 aatttggttt atttttagaa agtaaataga aaaccccaga tgttgtcgtt tcaggttgaa
    12841 ctaaagaatt agaattttcg tcatataaga aatattctcc agctgttcta ctatattcca
    12901 taccgtctcc atctattaaa gatgcctgat acgaagacaa agagaaaggt gatgttgctt
    12961 tattttttaa cttgtaagaa atagtagtac gatagtattt ttctggaaaa ttttctatt
//
LOCUS       DAZUMB010000075        12961 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000075 DAZUMB010000000
VERSION     DAZUMB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.076"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            584..1057
                     /locus_tag="ACG80L_001846"
     CDS             584..1057
                     /locus_tag="ACG80L_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS5024071.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1066..1293
                     /locus_tag="ACG80L_001847"
     CDS             1066..1293
                     /locus_tag="ACG80L_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024072.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1929..2300
                     /locus_tag="ACG80L_001848"
     CDS             1929..2300
                     /locus_tag="ACG80L_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024073.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2556..2798)
                     /locus_tag="ACG80L_001849"
     CDS             complement(2556..2798)
                     /locus_tag="ACG80L_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5024074.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2830..3732)
                     /locus_tag="ACG80L_001850"
     CDS             complement(2830..3732)
                     /locus_tag="ACG80L_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5024075.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3745..3933)
                     /locus_tag="ACG80L_001851"
     CDS             complement(3745..3933)
                     /locus_tag="ACG80L_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5024076.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3947..4510)
                     /gene="amaP"
                     /locus_tag="ACG80L_001852"
     CDS             complement(3947..4510)
                     /gene="amaP"
                     /locus_tag="ACG80L_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5024077.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4548..5441)
                     /locus_tag="ACG80L_001853"
     CDS             complement(4548..5441)
                     /locus_tag="ACG80L_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5024078.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5519..6442)
                     /locus_tag="ACG80L_001854"
     CDS             complement(5519..6442)
                     /locus_tag="ACG80L_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5024079.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6491..6841)
                     /locus_tag="ACG80L_001855"
     CDS             complement(6491..6841)
                     /locus_tag="ACG80L_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5024080.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6874..7776)
                     /locus_tag="ACG80L_001856"
     CDS             complement(6874..7776)
                     /locus_tag="ACG80L_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5024081.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7769..8626)
                     /locus_tag="ACG80L_001857"
     CDS             complement(7769..8626)
                     /locus_tag="ACG80L_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5024082.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8960..9769
                     /locus_tag="ACG80L_001858"
     CDS             8960..9769
                     /locus_tag="ACG80L_001858"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5024083.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9809..10306
                     /locus_tag="ACG80L_001859"
     CDS             9809..10306
                     /locus_tag="ACG80L_001859"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS5024084.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10691..10764)
                     /locus_tag="ACG80L_001860"
     tRNA            complement(10691..10764)
                     /locus_tag="ACG80L_001860"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10728..10730),aa:Ile,seq:cat)
     gene            10951..11274
                     /locus_tag="ACG80L_001861"
     CDS             10951..11274
                     /locus_tag="ACG80L_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024085.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11438..11692
                     /locus_tag="ACG80L_001862"
     CDS             11438..11692
                     /locus_tag="ACG80L_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024086.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11762..12007
                     /locus_tag="ACG80L_001863"
     CDS             11762..12007
                     /locus_tag="ACG80L_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5024087.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12083..12448
                     /locus_tag="ACG80L_001864"
     CDS             12083..12448
                     /locus_tag="ACG80L_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS5024088.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12509..>12961)
                     /locus_tag="ACG80L_001865"
     CDS             complement(12509..>12961)
                     /locus_tag="ACG80L_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024089.1"
                     /translation="FQGGNLMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEE
                     LKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYT
                     DVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         3760 a         2421 c         2188 g         4592 t
ORIGIN      
        1 gcaatccatt ttatttttct acttcttctt aatacctcta acacttttga tttataaact
       61 aatccaatgc atctacttgc gcttattaag ctatttcagc tagatagtac caccccatgc
      121 gtcaatgctt gattttcctt ttgtataaca ttgtataagc aataaaggac aaataagtgg
      181 tgattacacc aaaccattga ataagaaaaa ttctgatacg taaataaaca tgaacttgaa
      241 aaaattaatg aatcggttag ctactacgta gttgtctttt ttctcataca gttctgttaa
      301 cttacacttc tcttcgctag ataataaaaa atcctccaac tgtattgtcc aatataatcc
      361 caggcatcta aactagcatt atagaagaga cacacataga atctaaaata actggtttta
      421 aatgtttctt acaatcaaaa aaagctgtta aatgcactcc acaccttcat actctatcat
      481 tttttataaa cgatatattt ttattgtttt tcgataaaaa atagttgttt tacaataatt
      541 attctgctat attttagcta tcaaagataa aagagggata aaaatgaaat ttaaaacatt
      601 gctattaaaa tttgtcgttt taggtataag tgccgtattt attttgttcg gcgtactttt
      661 ttatactcag atcgctacaa gcgaaaaagg attcaatttt gattggcaga ccacattatt
      721 tgtttctgtc atttactttg cccttctcct agggatcgtt attgcttatt tcttattgaa
      781 actattgaaa aaaatcgaag ataaccaagc tttttccact caaagcttaa acatactaaa
      841 aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt tttggcttac ttcctaaatt
      901 tatgaaagta gcggatttag acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt
      961 attcattcct tttgcagtat acacattaac aagcgtgctg gaaaaactgc ttgaacatgc
     1021 gattttactt aagacagatc atgatttgac agtgtgaggt gcgacatggc aattatcgtt
     1081 aatttagatg tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg
     1141 ggcattacga tggcaaacat ctctattcta aaaaatggaa aagcaaaagc catccgtttt
     1201 tccactctcg aaaaaatctg ccaagttttg gagtgtcaac caggtgattt gttgacctat
     1261 aaaaatgact cgaatactat accagaagaa taaaataaag tagaacaaac agtaaataaa
     1321 aaataatctg aatgatagat gtgtgagact tttagaagtt atttacgtct attattcaga
     1381 ttttttattt cgattgaata ccgttttatc tataaataag atatgtactg actcatacaa
     1441 agggggcctt ttcttctcca tgtactatca cttttcctga tacaactggt ttcctgttac
     1501 gttgtagttg gatctgcttt tagtataata ctttattcat tccaccagac tgatagcagt
     1561 ttgagcagtc taaataatca tgtagtatta caggccattc tgtttcatac cttgaagtgc
     1621 ttaaaacttc agctaatggc ccattgtgtt aactcttact ccattctttt ttagtaagct
     1681 cccatatata ctgtaaagcc ttctatatat tatgtcatgt tgctttttaa catttactat
     1741 gtagttttga taaaatgatt tgaataacag tgatggacaa ttctttttta tttttaacat
     1801 tattttgata ataatgatta tttatttaaa aatacattag ataacatttg ttacgacacg
     1861 aaaaagcttc acaaacaaat atcgttattt taaaatgagg gcgaactcac acaattagga
     1921 ggaatagtat gccatttatt gtttactttt ttctttcatt actcgtgatc tatattccat
     1981 taccaacatt tttcctgctc gtcaatcgtc tagcagagca acatgatacg attgctgctc
     2041 ttacgaaact ttgtttactt ctatttgttt gtctagatta ctttacgagc tattattggc
     2101 taaaacagac aagagtaaaa aagagatatt atttcttcct tgtctttttc aatctctttg
     2161 agtttctgat ccattgcttt ttacttcatc agtatcatac gatccatttg tggatcatct
     2221 gggttttcaa agtggtcgta ttattttgta tgttcacctt tcctatctca agaaaattta
     2281 gggaggcatt gttttattaa agaagcttct ctgtatcaat aaagacacat ttaaggaaaa
     2341 acttttaaaa atctaattgg gaatttgccc gcataaataa gaggctgtga caaaagtctt
     2401 gtcacagcct cttattccgt ataaacagtg ttcaaaagct tgcatcttcg ataatatgcc
     2461 caaccaaatg aaaaatatga gaagctattt ttgtttggct gttcttatta cttgatgaag
     2521 aacagctttt tcacgacctc tttttaaaag tctttttaca gttttcgagc aaagaatgat
     2581 acgattagta ctaaaatgat ggcaccaatc aatgatggca cgattgccat tcctgcaata
     2641 cttggtcccc aagaacctaa taatgcttga ccaagtgctg atccaactaa acctgctacg
     2701 acatttgcaa taatccccat cgaagaacct ttattcgtga ttgctcctgc aattgctccg
     2761 ataattcctc cgacgataag tgaccaaata aatcccatgt ttcgttcctc ctaatgtttt
     2821 acttccttat tactgaaccc gtggttctgt attttctttt actttttgtg cgccgcttcc
     2881 agctgctgat tttactttat ctgtttgatt cgaagcaaat tcaccggttt ttttcgctgc
     2941 gtcagttact ttgtcttgca ccgtttcttg atctttttca aattcttctt ttgttttgat
     3001 gtccactacg ttagcgttga cttcgatcac ttctaaatgt gtcatatttt ttacttcttt
     3061 gccgattact tcttgcattt cttcaaagat tttttcgata tctttgccat attccacaat
     3121 gacatccata tcaactgcta cttgtttttt accgacttct gtttcgatcc cagccgttac
     3181 attatctgta ttcacaagtt ttcctgccac attagagaag aaaccaccgt ttgcagataa
     3241 taaaccgtct acttgttcaa ttgcgattcc gatgattttt tgcacaactt tatcttcata
     3301 attcagctca ccgcgcattg aatcttcctg ttcagaatca tgctttcctt ggttatttgt
     3361 atgatcagaa ccaagttttc cttgtccgtt tgtgtggtca gaactaagtt tttcttgtgc
     3421 tttcgcatgg tcagaaggat gtttttgctc ttccacaggt ttagaaggat cgattgcatc
     3481 atgttgttct ggaactttag attctggagt tcctggatga tgaggtgcat gcttttctgc
     3541 atcatgtttc ttctcttcta ctccttgttg aacgttaggg actactggat ctggatgtgc
     3601 agatactggt gctgttttgt tcttttcatc gttcacacca tttgattggt ttggtgtcgt
     3661 tgggtctgga tgtgcagaca ctggtttttg gttgttcatt ttattgttat tatttttgtt
     3721 attgttattc atggatgttt cctcctaaga tttaaaatag tgatctaaga ttttcgtttg
     3781 tttcagataa aaaccgatag cgacgccgat cgctgccatg atcaagatca ataaggtctt
     3841 gaaaaagccc acggtgatcc ataaaacggc taatatcaat ccaagtaccc caaagatgat
     3901 agggagcttg tatgtcgaaa atagttcttt catttttggt ccccccctat tctactcgtt
     3961 tgcggtcatt gcttcgttta gctggttctt caaagcctgt gtatttcacc gatactttca
     4021 ctggctcttt tgttcccaat atgtgttgta cttcattttc gatttctttc atcaatgttc
     4081 ctgttttacc aatgagtgct gaagttcgtt tcaactcccc ttttacattg acggcgatct
     4141 tgtttttcgt tgctttgaca tttacttttg gtgaatggat gaattcatct tcatgcaagt
     4201 gagaacgaac cattccttca attgcttttt tatccaacgt caactcccca tcaccatgtt
     4261 tcagtagaaa gtttccgatt gtttttggat aaaacaggaa aacaagtaac gtgataattg
     4321 tcaaaatagc aaatgcaacg gcaagccaaa atacatattg ttgcacgtat gaattagtga
     4381 tggaaaagaa tcgaaacttc tctagctgaa aaggcaatgg ataggttgca cttaatagta
     4441 tcgcaaacaa aagcacagat agcaaaagta atgttccgat cactccgatc gtttttacac
     4501 ctttattcat tttcgcacct cctcataggg gtgaaacgat caacttctta taagttgatc
     4561 gcttctccac cagttactcc gtaaatttgg gctgtaacat aacttgcttt attcgatgct
     4621 aagaaaacat aaactggtgc taattctcct ggctgacctg cacgttctag taaactttgc
     4681 tgaccaaatt cagggatcga atcagaaggt tgtccttcgt ctagttgtaa tggtgtccag
     4741 attggaccag gtgctacacc atttacacgg attcctttag gtgcaagctg ttttgctaaa
     4801 ccgatcgtaa agttagcgat tgaagctttt gttgctgcgt aatctaataa atgttcgctt
     4861 ggattgaatg cttgaacaga agttgttgtt acgattgctc ctcctgctgg gatatgcgga
     4921 acagcttctt tcactaaagc aaacatagcg atgatgttta tcataaatgt gtctttgact
     4981 tgctcaagtg gtaactcagc aatagactga caagaaattt gctgtcctgc gtttagtacc
     5041 atcgtatcta gcccgccaag ttcactcacg gctttatcta tgatttcttt aggtgcttgt
     5101 tcgtcacgaa gatcaaaagg tagtaagaca atcttacgtc ctgcatcttt cacgtatttt
     5161 gctacttcct ctgcatcttt ttcttctcct gggaagaatt gaagagcgat atctgcccct
     5221 tctctagcaa atgcaatcgc tgctgcacga ccgatccctg aatctccacc agtgattagt
     5281 acacggcggt tttctagttg attattgcct tcataggatt cttctccaca atcaggaaca
     5341 ggaatcatat tctgctgtaa agctggtgta tcctgatctt gtttaggaaa tttctccgta
     5401 tggtacaagt ttcgcgggtc gattaatttt ggttctgtca ttgctgtatt cctcctagtt
     5461 tttaaattgt attgccaaaa caaatttctt tgtttgatac ctgttttaaa tagattcttt
     5521 aatttgtata atctttccgg tataaatggt aacctaaaac tactgctgct atcaaagagc
     5581 caaagactac gagtataaag cctagagcag aacttttacc cggtaatcct cctgtattca
     5641 ttccccaaat ccctgcgaac aacgccggaa cggaaatgat taatgcagct gaatctagat
     5701 atttcatgat atgatttaga ttattgtcca tcatcccgtt gaatagatca ccaatactct
     5761 ctaataaatc tcgatagatc tcgatcattt tttctgtttg tcgctgtctc aaatggacat
     5821 catagagtag ctgttcatcg tctaaacgtt tgatgaaagt ttcagaattc cataattgat
     5881 ccattgtttc ccgttgatct cttaaggtat gatcgatata tacgatgtct ctttcaagat
     5941 ctgcctgttt atacagttct tcattttccg ttgttttacg agctgcttta tccaaagcgt
     6001 caattcgctt tttcatctct cttagttctt tcacgtaatg ggtgtaaata tctaataatg
     6061 tatacgtgat gatcttttca aacgagttta aatgatttcc atgctttgtc agcagccgat
     6121 ccacaaattc ggtttttcta tcgatacaag taatcaagag attatcggaa agtacaaaag
     6181 atattggcgt taattttttt tcgatacgat tatctgactg atctagatta atcactacca
     6241 aagaaaatgg atgctctagt ttcgtctcat aaaggcgctc catccgagaa acttcttccg
     6301 gatattcggt tcctacgaaa atatcatcag gaagctcgaa ttcttcgatt gcttgctcaa
     6361 tctcttcggg ctttgcacta tcaagtacga gccaattata ttttttttca tctgtttcta
     6421 ttacattctt ttcttcatcc acaaagtagt atctcaatgt atacctcctt tttggacttt
     6481 ctcgaaacta ttagctgaag aagcagttct ttaatttgat attataatcg agtactgcga
     6541 tttcattttc taatgaaaag ctgtcaagct gtttgttgaa gtccatgttc aatcgtttac
     6601 cgaaataatt ttgctctaat ttagatgtat agatgtccaa taatggatgc tcgattttag
     6661 tcgcatattc tccaaattcc gaaacaaaag gaatgatttg aaaaaagctg ccttttgcaa
     6721 aagcaccttt gtcccttaga aatggatcgg aatcattgat aagcgcgagt gcgatttttg
     6781 tattttcccc aaccttttca attaacgtct ctactgcatt tttagacatt gtaatttcca
     6841 ttcctactcc ccctcttcag ttgttgtctt ctattattta acaactacat ctgtttttgg
     6901 ttgaactttt gcatattttg gatatttagg gaatttagct acacaacgga aagccgtcat
     6961 ccctgcatat ttttcgtaat atttcttcgt tggcccttca aaatccttgc tccacggttt
     7021 cacatgtcct gaaaaatgga taatgtaagg attgttgcga gtttcttcgt attcacgttc
     7081 acctgctgct gttggatgtt tttttgcttt tgccatgata tagccttgtg cattccaacg
     7141 cggatgaagt ggtaaccaac ggtcatgtaa gatagcattt aatgcatctt ggtcatggaa
     7201 tcgtaatttt tctggattat gttcgatgaa atctaatact ttttgcgtaa tattttcatc
     7261 taaccatttt ttcacattga tcagcatcaa acctgagttg aaataacgca ttgatttagc
     7321 tgaaattttc attttttcca gtcgttgatg gaacccagcg tcttcaacag ctgctacgat
     7381 tgaatcaccg aagtcaagac gccataattt agagatatct tgtaaagcga tcatgtcgca
     7441 atccatatat aagatacgtt caacttctgt gccgcggaac aattccggaa tcgcaatacg
     7501 ataataagct gtttctggaa tacgatcgct gattaccaca tttgtaaaga attctttatt
     7561 gattttcaag aattccacat ctgagttcat tcgtgcgttt ttcactgaga agcgtaaaag
     7621 ctgtttactt tcaaaatcga tatcatcgtc aatcacatag aaccgaacaa atttagacgg
     7681 gttgcaatta tctaatactg agacaaataa tgcagctaaa tgtggtacga attttgtgtt
     7741 acaacttgcg actactgcta tttctttttt attcattgat ctcgcctact ttcgttagag
     7801 tactgtgtgc taattttttt aagtataaat tggtatacgg atgatctttc aacgtattcc
     7861 aaggtttatc atgtcctgta aagtgaacga tcgaaggttt ttcatttcct tctttatact
     7921 gacgttcata tttttcattt ggtgctggat gacgttcaaa aatcagcgaa gtctgcatgt
     7981 tccattttgg atgaagttgt tcccaatctt cataaagtac agcattcaaa gcatcttggt
     8041 cgtgatagat gattcgatcg ccattttcac tgaggtaatg gatagttttt tctgtaattt
     8101 cttttttatt ccattgatca atgtcaatca ccatcactcc tgaattgaag tacaagtcat
     8161 cggaatcgat tcctaatctt tccaaagctt tcacttggcc tggatcgata atagcgccga
     8221 ttgtttttcc atttaacggc tcatcatata gttttacgat atcgtctagt actaacacat
     8281 ctgaatctaa gtaaaggact ttcttataat cctctttagc taataagttt ggtaaagaaa
     8341 ttcttaagta agcggtagtt gtgatatggt cactgactag gaagtcttcg tagatatctt
     8401 tttccactgt caggaattgg atacttgcat tacttgaata tttatttacc gtttcttcca
     8461 atccttcttt actatattct gatagaccat cgtcaatcac ataaaatttt atgcgtcttg
     8521 ttttattgca attttctaat gcagttgcga tcatcacgct caagtaagga gcataatttt
     8581 cgtctgaagc agttaccaca ggaactacgc cgtatctctt ctccatcaaa atcactcctt
     8641 tttctaatct ttttctaacc tgttctacat ctagaatatt cctcatttgg ttttaaagca
     8701 aaatatatgc tttgttttca ttgaatattt taaaattttt ttatcacaaa acgcatcggt
     8761 aagcgctaac tattcgtttt tcatggcttt ttattcaaag gtattgacca aaaactacta
     8821 tatatagcca cctttttctt aagaatgttt tttgaaaaaa ttcataatga caaagcaagt
     8881 tagtttcatt tttttaaatt ctcatttaat cttaaggtaa caggaaaata gatatgtaaa
     8941 aagaaagtag gaagataaaa tgaaactagc agcgattgat ttagacggta ccttgcttga
     9001 tcgaaatgga aagataccag aagaaaatat ccaagcactt cggaaatttg atgaaaatgg
     9061 cggaatcgta accgtcgcta ccggaaggaa cagcatttct gctaaagata ttttttccga
     9121 attaggaatc ggtggctatt tgatttcttc caatggttca ctgattgctg aaatgaagga
     9181 tggaaaaatg gcgaaagttc tcagacgttc aaaaatagat agcgatacac tgagaaaagc
     9241 cttcttctat gctcaagaag cgaaaatatc catcattgct agccgagaaa cccaagatga
     9301 tcagctgaca ttcgatgaat caaatctagt caaagatgac ccttattatg aacatttcaa
     9361 tttacagaac cattcttttg atcaaatttt aaatatgttg gaagatcctt ctcttagcta
     9421 tcttaaatta gcattaacag ataagagtga agaaaaacta cagaagctac aaagagatct
     9481 gcaagcagat ggtatccaat cagtattctc tgatccgcat ttcttagaaa tcacgcctac
     9541 aggtatcact aaagctcatt cacttgtttt tctggcggat taccttggaa tcgaatcgag
     9601 tgacatcatg gcttttggcg atcaagaaaa cgacattgct atgttgaatt acagcgggtt
     9661 gagtattgcg atggggaatg cacagcgaca agtaaaagaa attgcaaacc aagtaacact
     9721 gacgaatgaa gaagctggtg ttgcttatat tttaaaaaac ttctattaaa aagaataaaa
     9781 tgacgatatg aatgtaggag gaaagaaaat gttagttatt attgatatgc aaaatcatat
     9841 tcttgatcca aacagcgaat tttatattcc agagtcagat caattggcag aacgtattgc
     9901 tcaaagatta gctaaagcac gccaaaataa tgaatatatt ctcttcacac gggatatacc
     9961 agttgaactg aaggacaaag aagaagaacg agaagatttg caattgatcc ctcttctttc
    10021 tccacaagaa aatgaacgag tgattaaaaa gtattacttt acgattccac ctgaaacttt
    10081 agctgaaatc caggaaactt tatttgaaag caaagaagaa caaaaagaga tcgaagtagt
    10141 cgggattgaa acaaacttgt gtgtactctc taatctgata ggcctgcaga gtgcttttcc
    10201 tgaagcagac ttttttgttg atccaatgtt agtctccagt agaaaacacg gagaatccgc
    10261 tttagagctg ttgaaagatt tcaacgtctc tatcgtcgaa tcataaaaat atctatcttc
    10321 atttacgata tttaacacac aataaaaaaa gcacgcagaa ctgttttagt cctacgtgct
    10381 ttttctatca aagtagattg gcttttttat gacacagagt gaaacggcag gctcacgacc
    10441 tctttcttaa atggtgttca atcagctgac cctcggtatt agcttaacaa acggcaaaat
    10501 atgaggaact atttttccgt ttgttaagct aatactcagg tcaaaacgct gatttcacaa
    10561 cctcttcctt aaacggtatt caaaagcttg catctgcagt aataagaaca gccaagtaaa
    10621 aacataagaa gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca
    10681 caacctctta tgtaccttgt aggactcgaa cctacgaccg gacggttatg agccgtctgc
    10741 tctaaccaac tgagctaaag gtacatcata aataaaattt atttttgtta cttttcaact
    10801 atagcacgaa tgacaaactc tcacaagttt caaaatataa gtaaaataat attgttattc
    10861 ttatttcttt tacttgtgtc ataactattt tgttacttta atttttattt gcttatttta
    10921 gaaaaaatgt tattctttta tttattctca atgttaggag tgattatttt gccatttatt
    10981 gcgattttat tcgatttagt tgcggcagca gcttattttc tccaattaaa ttatcaaacc
    11041 tcaggaattc tgtttattgg aatgattttt caaggtgtga ttaccctttt gcttcttgtc
    11101 atgatgctca gctataaagg aaaaagatat gcccgtattc aaacaaaagt atttgttaaa
    11161 tatgtttcca ttcgctatgg gatcatcatt ctttcctttt tagtgaatgc gatcgtcctc
    11221 tttttatatg tattgaatta cttagatatc aatccattga ttttttctcg atagataaga
    11281 cacattcagc ggaacagttc ctgcttatca gcagaaaact gttctgcttt ttcatttttc
    11341 attgatctat tcttctttta ttttcctagc atttcatttg tgatgtatgc aataacatgt
    11401 tatcttttat tatatgaatc gttgaggagt ccgttacatg attacgttgg tgagagtttt
    11461 gttttgggtg cctgcggtcg cactggtcgc aagtattgtt tatcttatga attggaacaa
    11521 agaacggttt tatttagcta ttttgactct tccggctatc tactttatgt ggaaagtttt
    11581 taattacaac tactttgaac ccgatagtgt ttttatagaa gaattatcag gactggtttt
    11641 gtctttattg atcgtcattc tttatttgat acgtttaaat aaaaaacatt gagtactatt
    11701 gattgcatgt gtagctttct atagtcatta taactattct actataatga aaggtgatta
    11761 aatgagtatc gaagaagcta ttttactgga tactttacgt tgtcgtctga atttcttaag
    11821 aactggccga tctttatctc aagtagctgt ttctagcgcg ttaagtattc ctttattcaa
    11881 atatcgtaag tacgaagaag gagaagaaat tccaagcatc attgaattaa tctatatcgc
    11941 tgacttttac gatatttccg ttgattacct aattggtcgc tcagaaacag caagcaaact
    12001 gaagtgattt tttccagcct tggtgctggt tttttcattt aacgtctaag gttaacaatc
    12061 tatatataaa ggagacattt ctatgcgatt atggcatgaa aaattgattt caaagcttcc
    12121 tcgtcaacag cttttaggac aacatcggga atgctgtgca ttaagaggaa atggctgggg
    12181 aaagaaacat gctactgtgg attatgtttt tcactattct ccttatagat tattccaata
    12241 tcatcagctt gtcatggatg aaatggaaaa acgtggttac agcccagcac cagagtggaa
    12301 aaatcctatg tatcgtggca aatcatgcga agcatttttt gatttaccta ctgtagagcg
    12361 caacgtgccg atttatccgg aacacgatga agtttatcta gcagaatgtt tagcaaattt
    12421 ggagcaaaaa ggaatttatc ttacctaata taactagaaa agccttctat ctcctcttac
    12481 ttcattaaaa agctttaacc atcttagatc aagctaaaat gtcgtaagtt ttagggttat
    12541 cttcatcctc acgtgcaaaa ctaaaagcgt actctagttt tggttcatcg ccaacttccc
    12601 tagacataaa tgaacatctc ctgacgtggt ttaaaagtac cgcatcttcg acgtctgtat
    12661 acgatgcaca gcacagattg cttcttttga tctttctttt gatttttctg ttgcttcttc
    12721 tacaccttct tcgattacta catcgatgct tacaattgct tctgcctaat attcccatgc
    12781 atcgtcgatt taagcctctg tagtcactgt gatcgtttcc acattttaac tcctctgtcg
    12841 tggcttcagt tgattttgtg atcagttcag ttgctaagcc agagtcagaa caacctgcca
    12901 ttgctagcgt taatattgat agtaaaaata gtttttttat cataaggttc cccccctgaa
    12961 a
//
LOCUS       DAZUMB010000076        12943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000076 DAZUMB010000000
VERSION     DAZUMB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.077"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG80L_001866"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG80L_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS5024090.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG80L_001867"
     CDS             complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG80L_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS5024091.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="ACG80L_001868"
     CDS             complement(3218..5533)
                     /locus_tag="ACG80L_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5024092.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="ACG80L_001869"
     CDS             complement(5718..6578)
                     /locus_tag="ACG80L_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS5024093.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7269)
                     /locus_tag="ACG80L_001870"
                     /pseudo
     CDS             complement(6637..7269)
                     /locus_tag="ACG80L_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7436..8197
                     /locus_tag="ACG80L_001871"
     CDS             7436..8197
                     /locus_tag="ACG80L_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5024094.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="ACG80L_001872"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="ACG80L_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5024095.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG80L_001873"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG80L_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5024096.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="ACG80L_001874"
     CDS             12557..12913
                     /locus_tag="ACG80L_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5024097.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4222 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DAZUMB010000077        12746 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000077 DAZUMB010000000
VERSION     DAZUMB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.078"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(308..670)
                     /locus_tag="ACG80L_001875"
     CDS             complement(308..670)
                     /locus_tag="ACG80L_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024098.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(744..1124)
                     /locus_tag="ACG80L_001876"
     CDS             complement(744..1124)
                     /locus_tag="ACG80L_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS5024099.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1206..2381
                     /locus_tag="ACG80L_001877"
     CDS             1206..2381
                     /locus_tag="ACG80L_001877"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS5024100.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2458..3042)
                     /locus_tag="ACG80L_001878"
     CDS             complement(2458..3042)
                     /locus_tag="ACG80L_001878"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5024101.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3243..4241
                     /locus_tag="ACG80L_001879"
     CDS             3243..4241
                     /locus_tag="ACG80L_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5024102.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4353..4862
                     /locus_tag="ACG80L_001880"
     CDS             4353..4862
                     /locus_tag="ACG80L_001880"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS5024103.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4892..5551)
                     /locus_tag="ACG80L_001881"
     CDS             complement(4892..5551)
                     /locus_tag="ACG80L_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS5024104.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5553..6317)
                     /locus_tag="ACG80L_001882"
     CDS             complement(5553..6317)
                     /locus_tag="ACG80L_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS5024105.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6325..7002)
                     /locus_tag="ACG80L_001883"
     CDS             complement(6325..7002)
                     /locus_tag="ACG80L_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS5024106.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7039..8424)
                     /locus_tag="ACG80L_001884"
     CDS             complement(7039..8424)
                     /locus_tag="ACG80L_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS5024107.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8393..8647)
                     /locus_tag="ACG80L_001885"
     CDS             complement(8393..8647)
                     /locus_tag="ACG80L_001885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024108.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8634..8718)
                     /gene="ffs"
                     /locus_tag="ACG80L_001886"
     ncRNA           complement(8634..8718)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG80L_001886"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="ACG80L_001887"
     CDS             complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="ACG80L_001887"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS5024109.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9363..10220
                     /locus_tag="ACG80L_001888"
     CDS             9363..10220
                     /locus_tag="ACG80L_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024110.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10301..10636)
                     /locus_tag="ACG80L_001889"
     CDS             complement(10301..10636)
                     /locus_tag="ACG80L_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024111.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10703..12073)
                     /locus_tag="ACG80L_001890"
     CDS             complement(10703..12073)
                     /locus_tag="ACG80L_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS5024112.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12151..12687)
                     /locus_tag="ACG80L_001891"
     CDS             complement(12151..12687)
                     /locus_tag="ACG80L_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5024113.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3797 a         2584 c         2121 g         4244 t
ORIGIN      
        1 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
       61 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      121 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      181 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      241 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      301 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      361 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      421 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      481 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      541 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      601 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      661 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      721 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
      781 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
      841 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
      901 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
      961 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1021 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1081 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1141 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1201 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1261 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1321 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1381 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1441 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1501 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1561 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1621 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1681 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1741 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     1801 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     1861 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     1921 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     1981 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2041 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2101 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2161 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2221 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2281 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2341 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2401 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2461 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2521 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2581 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2641 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2701 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     2761 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     2821 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     2881 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     2941 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3001 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3061 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3121 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3181 aaacattttt gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt
     3241 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3301 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3361 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3421 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3481 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3541 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3601 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3661 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3721 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     3781 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     3841 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     3901 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     3961 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4021 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4081 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4141 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4201 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4261 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4321 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4381 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4441 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4501 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4561 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4621 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4681 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4741 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     4801 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     4861 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     4921 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     4981 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5041 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5101 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5161 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5221 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5281 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5341 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5401 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5461 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5521 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5581 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5641 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5701 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     5761 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     5821 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     5881 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     5941 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6001 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6061 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6121 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6181 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6241 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6301 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6361 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6421 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6481 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6541 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6601 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6661 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6721 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     6781 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     6841 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     6901 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     6961 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7021 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7081 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7141 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7201 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7261 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7321 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7381 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7441 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7501 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7561 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7621 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7681 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7741 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     7801 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     7861 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     7921 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     7981 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8041 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8101 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8161 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8221 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8281 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8341 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8401 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8461 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8521 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8581 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8641 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8701 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     8761 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     8821 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     8881 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     8941 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     9001 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     9061 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     9121 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     9181 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     9241 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     9301 tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca
     9361 agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag
     9421 catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag
     9481 attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc
     9541 aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt caagaaaaaa
     9601 gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc
     9661 aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag
     9721 atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta
     9781 tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc
     9841 attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag
     9901 agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt
     9961 actatacaaa agatactgta aaagatagta agttgatcaa aagttacata gaactgatca
    10021 aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct
    10081 ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc
    10141 aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat
    10201 tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac
    10261 cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa
    10321 aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag
    10381 agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac
    10441 tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga
    10501 cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg
    10561 atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt
    10621 ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa
    10681 aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta
    10741 ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc
    10801 atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt
    10861 gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc
    10921 taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta
    10981 aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa
    11041 gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct
    11101 cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt
    11161 gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata
    11221 tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg
    11281 attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga
    11341 tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt
    11401 ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag
    11461 attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac
    11521 aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg
    11581 gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa
    11641 tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag
    11701 cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg
    11761 gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc
    11821 cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg
    11881 gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta
    11941 gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg
    12001 atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg
    12061 cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa
    12121 ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc
    12181 caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc
    12241 ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga
    12301 cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag
    12361 cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa
    12421 aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta
    12481 gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt
    12541 gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt
    12601 aattgacttc attccatatt cttccacgta taccatactt ttgaacagca aatattcttt
    12661 actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa
    12721 taaataacat aaataattct aaaaat
//
LOCUS       DAZUMB010000078        12734 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000078 DAZUMB010000000
VERSION     DAZUMB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.079"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG80L_001892"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG80L_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5024114.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACG80L_001893"
     CDS             complement(322..891)
                     /locus_tag="ACG80L_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS5024115.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACG80L_001894"
     CDS             1077..1979
                     /locus_tag="ACG80L_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5024116.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG80L_001895"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG80L_001895"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS5024117.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG80L_001896"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG80L_001896"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS5024118.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG80L_001897"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG80L_001897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS5024119.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACG80L_001898"
     CDS             8547..8732
                     /locus_tag="ACG80L_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS5024120.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACG80L_001899"
     CDS             complement(8794..9990)
                     /locus_tag="ACG80L_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5024121.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACG80L_001900"
     CDS             complement(10160..11596)
                     /locus_tag="ACG80L_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5024122.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACG80L_001901"
     CDS             11889..12698
                     /locus_tag="ACG80L_001901"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5024123.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAZUMB010000079        12553 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000079 DAZUMB010000000
VERSION     DAZUMB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.081"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..201
                     /locus_tag="ACG80L_001902"
     CDS             <1..201
                     /locus_tag="ACG80L_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS5024124.1"
                     /translation="IQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKR
                     EAKLIIGISSTVTDYISGYLDK"
     gene            262..762
                     /locus_tag="ACG80L_001903"
     CDS             262..762
                     /locus_tag="ACG80L_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5024125.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            818..1456
                     /locus_tag="ACG80L_001904"
     CDS             818..1456
                     /locus_tag="ACG80L_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFS5024126.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1528..1923
                     /locus_tag="ACG80L_001905"
     CDS             1528..1923
                     /locus_tag="ACG80L_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5024127.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            1907..4360
                     /locus_tag="ACG80L_001906"
     CDS             1907..4360
                     /locus_tag="ACG80L_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5024128.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4357..6384
                     /locus_tag="ACG80L_001907"
     CDS             4357..6384
                     /locus_tag="ACG80L_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5024129.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6381..7403
                     /locus_tag="ACG80L_001908"
     CDS             6381..7403
                     /locus_tag="ACG80L_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5024130.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7420..8349
                     /locus_tag="ACG80L_001909"
     CDS             7420..8349
                     /locus_tag="ACG80L_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5024131.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            8663..8710
                     /locus_tag="ACG80L_001910"
                     /pseudo
     CDS             8663..8710
                     /locus_tag="ACG80L_001910"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8726..9526
                     /locus_tag="ACG80L_001911"
                     /pseudo
     CDS             8726..9526
                     /locus_tag="ACG80L_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            9764..10000
                     /locus_tag="ACG80L_001912"
     CDS             9764..10000
                     /locus_tag="ACG80L_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024132.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10007..11959
                     /locus_tag="ACG80L_001913"
     CDS             10007..11959
                     /locus_tag="ACG80L_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5024133.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12031..12528)
                     /gene="dfrG"
                     /locus_tag="ACG80L_001914"
     CDS             complement(12031..12528)
                     /gene="dfrG"
                     /locus_tag="ACG80L_001914"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5024134.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         4309 a         2093 c         2450 g         3701 t
ORIGIN      
        1 attcaaccta ctgaacttgc tgatatagtg aagacaacga taaggtcatc aaatggaatg
       61 attagctctc ttaatgaagc aagaaatagg cattcacttg cacaccctaa tacaaatatt
      121 attggaaaga gagaagcaaa actaataata ggtatttcaa gtacagttac agattatatt
      181 agcggatacc ttgataagta gcgtcattaa ctgattgcta ctttttttat gactattttt
      241 ataaaaagga tgtgataaga attggaaatg aaagtctatg tggcaaattt aggacggtat
      301 aatgaaggtg aattagtcgg agcatggttt actccaccaa ttgatgagga agaaatggca
      361 gaaagaatcg gtttaaatga ggactacgaa gaatatgcaa tacatgattt tgagcttcct
      421 tttgatgtgg acgaatacac gccgattagt gaaatcaacc gtctatgtga agccattcaa
      481 gaaatcgaag gtacacctat ttataatgaa ctcaaagaaa ttcaaggcat gtggtttagt
      541 agcttggaag aattactgga aaacaaagag gatattcatt gttatagtga ttgtgattcg
      601 atggaagatg tcgcccgtta ttacgtggaa gaaacaggac agcttggtga agtcccttcc
      661 aatttacaaa attatattga ctaccaatct ttgggacgtg atatggaaat agagggtaat
      721 tacctagtta cctctcatgg ggtatttgaa tattgtcagt aagtttaaca atccgtgtta
      781 taattaaatt aataaatagc acggagaggt ataaattatg gtaaatgtat ttgaaaatct
      841 taaactatta aaactagaca tgaaagataa aggctgggtg atagattcct tttactttcg
      901 ttacaaacag caaaattata ttgtacttgt taagctattt gaaaaagagg aaaaagttcc
      961 agaatatgct ctgttaaaac tagaattttt aaaggaaaac gatttttcag atatgctagc
     1021 tgtctatgct aattcggtaa aattatatac agacacgaaa acaataagag aatattttgg
     1081 tatcgaatac agtagtaact tgggagatgt tttatttcaa tttagccaaa ctcttgctcg
     1141 atttattccg acagaagtta gtgagaagaa aaatgaagac caaaaagaag ccatgtgttt
     1201 ctcattaagt caaagtgatt cagaagatcc cagaaaaaaa tattgttttt cagttagaag
     1261 aaatccttta aagggaaatg gtgagctggg aaaacgaagt ccttttaatg acaataaaac
     1321 gagattgtat agacccagct tatatgaacg attagggtca gataccaatt taagtttcag
     1381 atattcaatg aatccagatg atgaagaaac tgatgaaggt attattgcaa aatggacaaa
     1441 aaataaaatt gaatagttaa agcattgaaa gagcaatcac ctattttgat tgttcttttt
     1501 ctatttttac ggaaaggacg tgaagacatg aaaaaaataa gaagttacac cagtatttgg
     1561 tcagtagaaa aagtgattta tgcgattaat gatttacaac tccctttccc aattaccttt
     1621 acgcaaatga catggtttat cgtttctttg tttgtcgtca tactctttgg cagtttacca
     1681 ccactttctc tgattgacgg agcactactc aaatacttag ggattcccat tgttctcact
     1741 gggttcatgt gccaaaagac ctttgacgga aagaaaccct atggatttct aaagtctatc
     1801 attacctatg ctctacgccc taaagtaacc tacgctggca aacaaatacg attaaaaaaa
     1861 gaaacaatga atgaatcgat tacgattgta aggagtgaac aactagatgg catatccgat
     1921 taaatatatg gaagataatc tcgtatttaa tcatgatggc gaagtattcg cttattatga
     1981 attaattccc tataattaca gttttttaag ttccgatgaa aaaattcaag ttcatgataa
     2041 tttctgtcaa ttgattgccc aaaatcgtga tggcaagatt catgctctgc aactagcaac
     2101 cgaatcaagt attcgagcca cacaggaaca atcgaaaaaa gaaatcactg gacgattaaa
     2161 agagatagct tacgaaaaaa tagatgaaca gaccgaagcg ttaatttcta tgattggtga
     2221 caatcaagta gactatcgct tttttattgg ttttaaactc tacctcaatg aacaggaagt
     2281 atccgtaaaa aatgttggga aagaaattca aaaagcattt gcggaattct ttcatggtgt
     2341 gaatcacaaa ttaatgggcg attttgtttc tatgcctatg gcggaaatcg aacgcttttc
     2401 taagatggaa cagttgttag aaagtaaatt atccagacgc tttcagattc gtaaattgga
     2461 gaaagatgat tttggttata ttattgaaca tctttacggg caaacaggtg tcccttttga
     2521 tgactacgac taccatttac cgaaacgata cgaagataaa aaagtgttgg taaagaaata
     2581 cgatattctc aaacccactc gctgtttaat cgaagaaaat caacgctatt tacggattga
     2641 acaggaagat accactactt atgtggcgta ttttacaatt aatagtattg ttggtgaatt
     2701 ggatttccct aatagtgaaa ttttttatta ccagcaacag cagtttactt tccccgttga
     2761 taccagtatg aatgttgaaa ttgtcgccaa taaaaaagcc ttatctaccg ttcgcaataa
     2821 aaagaaagaa ctgaaagatt tagatagcca cgcatgggaa agcgacaacg aaaccagtac
     2881 taatgtggtg gacgctttag aatctgtcga tgaattagaa agtaatttag accaaaccaa
     2941 agaatctatg tacaaactaa gctatgtggt acgggtatca gcaaagaatt tggacgagct
     3001 gaaacaacgt tgtgatgaag taaaagattt ttatgatgat ctgtctatca aattagtacg
     3061 accttttggc gatatgctag gcttgcataa tgaatttatt ccctcaagca agcgatatat
     3121 caatgactat attcaatatg tcacgtcgga ttttttggct ggtcttggtt tcggtgctac
     3181 acaaatgatt ggtgaaaatt ctggtattta cgtcggttac aatctcgata caggcagaaa
     3241 tatttatctc caacctagcc ttgcaagtca aggaattaaa ggctctgtaa caaatgcatt
     3301 agcgtccgct tttattggtt cattaggtgg cggaaaatca ttctctaaca atatgctggt
     3361 ctattattca gtactctttg gcggacaggc aaccattgtt gacccaaaag ctgaacgtgg
     3421 tgaatggaaa gaaaccttac ctgatattgc ccatgaaatc aatattgtta atctcacgag
     3481 tgagaacgac aataaagggt tacttgaccc ttatgtgatt ttaaaactcc cgaaagattc
     3541 agaaagcctt gccattgata ttctcacgtt cttaacaggt atttccagcc gagatagcga
     3601 gaagtttcct gtcctgcgta aagctattcg gagtgtaact caaagcaaac aacgagggtt
     3661 gttattggtg attgatgaat tgcgaaaaga agatacgccc atcagcaata gtattgctga
     3721 ccatatcgaa agctttgtcg attatgattt tgcccatctg ttattcagtg atggcagtgt
     3781 cacccaatcc attagcttgg aaaaacaact caatattatt caagtggcag atttggtctt
     3841 acccgattcc aatagctctt ttgaagaata cactaccatg gaattattaa gtgtggcaat
     3901 gctgattgtc attagtacct ttgctttaga ctttatccac tctgaccgtt ccatttttaa
     3961 aattgttgac cttgatgagg cttggagttt tttacaagtg gcacaaggaa aagctctatc
     4021 tatgcgattg gttcgagcag gacgtgccat gaatgcaggg gtctatttcg ttactcaaaa
     4081 cgcggacgat ttactagatg aaaagatgaa gaataatatt ggcttgaaat ttgcctttcg
     4141 ttccactgac ctagtggaaa tcaaaaagac cttagaattt tttggtgtgg acaaggaaga
     4201 tgaaaacaat cagaaacgct tacgtgaatt agaaaatgga caatgtttag tttcagacat
     4261 ctatggtcgt gtaggggtta tgcagtttca cccgattttt gaagagttgc ttcatgcctt
     4321 tgataccaga ccgcctgtgc gaaatgaggt ggaagaatga cacaaaaaca aaagaaattt
     4381 cttcactata gttggataac gctcctttgt gtgggcgtta ttttactttt attaggcacg
     4441 ctgggcaatg cagtacaagc gacaggctta gttgatgaaa ccattgatac aagcaatgaa
     4501 tactccaagt atggtttgaa tcaatatcag cttgattatt atgtggacaa tagctggggc
     4561 tggcttccat ggaattggag tgatggcata ggacaatcgg tgatgtatgg attgtatgcc
     4621 attactaatt ttatttggac aatttctctt tatctatcga acgccacagg ttatttggtg
     4681 aaagaagcct atgccttaga tttcattagt caaacatccc aagcaattgg cgaaaacatg
     4741 cagacgattg caggcattac accgaatggc ttttccagtt cagggtttta tgtcggtttc
     4801 ctccttcttt ttattctcat tgttggaatc tatgttgcct atacaggatt ggttaagcga
     4861 gaaacgacga aagtgatacg agctgtttta aactttgtcg tggtgtttat tctatcggct
     4921 tcctttattg cgtatgcacc cgattatatt gcgaaaatca atgatttttc caaagatgtc
     4981 agtaccgcca gtctgaatat tgggacaaaa attgttcttc ctaacacgga tagtcaaggg
     5041 aaagacagtg tggacatgat acgtgacagc ttattctctg ttcaagtaaa gcaaccatgg
     5101 ctattgcttc aatatggctc cactgatgta gaagcgttag gggaagaacg tgtggaaagt
     5161 ttattgtcta ccagtccaga tacaaacaat ggtgaagata gagaaaatgt agtgattgaa
     5221 gaaattgaag atcatgacaa tacctattta accctaccga agacgatttc cagattagga
     5281 acagtcttct ttttgtttgt attcaatatt ggtatctcga tttttgtttt ccttctaaca
     5341 ggaatcatga ttttttcgca agtgctattt attattttcg ctatgttttt gccaatcagt
     5401 ttcctattaa gtatgttgcc gagttttgat ggcatgggca ggcatgccat taccaagtta
     5461 tttaatgtga ttatgacaag ggctggtatt accttgatta ttaccgttgc ctttagtatt
     5521 tccactatgc tttattcgct gtcagcaagt tctccctttt tcatgattat gtttttacaa
     5581 attgtgacct ttgcagggat ttattttaaa ctgggcgatt taatgagcct attcacttta
     5641 caaagcaacg actctcaaaa catgggcaga caagtgtttc gcagacctcg tcaaatgatg
     5701 aatcgacaaa ccagaagatt tcaacgcagt atgagtaaga tttttcgtgg aaaagcaggg
     5761 tcaacgaagc aaaagacaat gacaacaaag cagaatcaaa ccaataaagc taaccatact
     5821 cgtcaaaatg aacggactac accaaagaaa gaaacagcac tttccaatgt gaaaaagcaa
     5881 caaggcggta tcgggcaacg attaggtgaa aagacagccc atgtattgga cacgaaaaat
     5941 cgtctagtca ataaaagaaa gcaggcaaaa caacagatac aggcgacacc aacccatgtc
     6001 aaatatgccc ttcatcaagg aaaagagaat gtcactcata atgtaacaga ctttaaacaa
     6061 tctatggcag ataccaaagc tcaacgcaaa caacagcgta tgaaccagca ggagcaaaaa
     6121 cgaaagaaca ttgctcaaaa gcgtttggag ctggataaag caaaagaaaa aagacgaaaa
     6181 gaaaacccat catctcctgt tcagaataac cagcagcatg ttcgcaagcg tcagcaaacc
     6241 gtcacgcctg ttttgtcaca aaacactaaa cgtgaaaatc atcagcgacc attgaaaaaa
     6301 gagcaaaaac aaaagacacc accgaagcaa ttcaatcaga ggaagctaaa taaaagaacg
     6361 gataagcgag gacaacaaac atgaagctaa aaataacagt gatttgtagt gtggtcttct
     6421 ctctcttctt ttttctctta ttattcctag tcattttctt tgcagatgat acggacagtg
     6481 gcgaaaacaa caaggattct tctattccac aaggcggtgt gaccgtttca ccagaagtgt
     6541 tggcacaccg tccgttgatt gaaaaatatg gtaaggaata tgggattgaa gactatgttt
     6601 cttatatctt agccattatg caagtggaat cgggcggtac agcagaagat gttatgcaaa
     6661 gttccgaatc tttgggttta ccgcccaaca gtttgagtac cgaagagtcc atcaaacaag
     6721 gggttaaata tttcagtgaa ttattaacca gtgcagaaca acaaggagtg gatatagatt
     6781 ctgttatcca atcttataat tatggcggtg gctttttaaa ttatgtgagg agtcatggga
     6841 aaaaatatac ctatgaatta gccgaacagt tttctaaaga gaagtcgggt ggtcaaaaag
     6901 cagactatcc caatcctata gccatacctg tgaatggtgg ttggcgatat aactatggaa
     6961 atcagtttta tgttcagttg gtttcacaat acctaaccga cacttcccca acagaatttg
     7021 atgatgaaac cgtgcaagtc attatggacg aagccttgaa atacgaaggg tttccctatg
     7081 tctttggagg ggcgtcgcct accacctctt ttgattgtag tggcttaata caatgggtgt
     7141 atgacaaagc tggcatttct ctccctcgtg tcgcccaaga tcagtatgac gctacgcaag
     7201 aaatctctat ggaagaagcc caagcaggcg accttatctt ctttcattca acctataatg
     7261 caggcacata tgtgactcat gtggcgatat atttagaggg aaatcgcttt tatcatgcag
     7321 gcgacccaat cggttatggc gacttaagca gtcgttattg gcaagaccat ctgattgggg
     7381 ctagacgtgt tattcataac taagaaagga atgaatcaaa tgattataaa aatcgagcga
     7441 aaagaaaaga aggagaaacc aaagaaatct aaaaaaccga gcaagcaaag aaaagttccg
     7501 atgattaaag ttggcacgca taagaaatta acctttgttt tatgggtttt attgattggt
     7561 agtgtcggat ttggaatcta taaaaatttt actgccattg acactcatac tgtccgtgaa
     7621 acagaaatca ttaaacagca gattgtcgat accaaccaag tagaaagttt tgtgaaatcc
     7681 tttgcgaaag actacttttc gtggcaacaa tcccaagaag ccattgataa acgaaatgaa
     7741 aaattgaccc attatttgac ggaagaattg caggtattaa atgaagaaat gattcgtaag
     7801 gatattccta ctagttctag tgtgaacgat atacaggtgt ggcaggtttc tcaagtgaat
     7861 gagaacacct ttgaggtact gttttctgtt gaacaagtta ttaccgaaga caaagacaag
     7921 gaaactatat cgtctagctt tcatgtggtt gtccatatag atgaatcaga taatatggtt
     7981 attatcaaaa atccgacaat gagtaagaaa cctcaaaaat cggactacca acccaaacag
     8041 ctagaaagtg accatacagt agatacagaa acgatggacg aaatcactag ctttttagaa
     8101 acgttcttcc agctctatcc taccgccaca gaaaaagaat tgacttacta tgtaagtaat
     8161 catgtgttac ctatgattaa caaggaatat gttttcgagg aattggtgaa tcctatcttt
     8221 accagaaaag ataaccaagt aatcgtgaat gtcgctgtga aatatttaga tcaagaaaca
     8281 aaggcaaccc aaatctcgca atttgagttg atattagaaa aacaagataa ttggaagatt
     8341 gtaaaataac aaatattggt acatgattac agatactttg taatcatgta ctctttttga
     8401 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtgactatt
     8461 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagcg
     8521 ggaacaaata attagatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
     8581 atacctttat catgtgattc taaagtatcc agagaatatc tgtatgcttt gtatacctat
     8641 ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt
     8701 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     8761 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     8821 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     8881 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     8941 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     9001 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     9061 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     9121 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     9181 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     9241 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     9301 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgttc
     9361 tctgttccct ctttatcatg gaagtgcaaa aagtaatata gggattgata accttataga
     9421 agttattact aataaatttt attcatcaac acatcgaggt ccgtctgaac tttgcggaaa
     9481 tgttttcaaa attgaatata caaaaaaaag acaacgtctt gcatattaag tagttcagtt
     9541 ttggagtaca aaaagtgaaa atgtaaacct aaatgagtat caaattgcaa ctgaatttga
     9601 tactcgtttt ttattgttaa atcaacgttt gttgttaatt tacaccacaa aatgtaagtt
     9661 aaaatgtata taaaaatgcg aacgtgaaat aattattgta ggaatgcaaa ttggtgtgtt
     9721 aaaatggtta caatttacta agcaaaaagg ggtgcatttt cctatgcaat ttcttgagcc
     9781 aaaaaataag aacgctaagt ctgtggattg ggaaatatca gagcaagtaa gggtgatagt
     9841 taagcaatac gctgaatatg cagagcgtac tgaaagcgaa gcagtggatg aattcttatt
     9901 gaacatttta gacgataaga agtttattga atggattgcc aacaagcgaa gtaataagcg
     9961 gattgttgaa aaaatgggta tcaaagatag agtgggttga tagacaatga ccaagcttaa
    10021 aagattagcc acgaaggaag atgaaattgt agatgttaaa attccgattt caaacgaaga
    10081 attaaaagat agggctaaac aatatgattt attaactcca aaacgatttg caacaaggta
    10141 taacaaaatg ttgttcttgc caacgtcttt taaatggaac ggttctgaat atcccattca
    10201 atataattac tgcatcaatc cgttttgttg taattttggg aaagaacagc acaaattcaa
    10261 agatgtaaaa gggaaaccaa gtcgatataa aatgactggt agttccaaag ataaaggtca
    10321 taaaggtatg tactgcaatg acaatcctat tggcagaggt gtatcgcaaa actgtacagt
    10381 aaccccttta tctaattggt ctgttgttga agaaattaaa cgccttatag aaatcaatag
    10441 catccaagat gttgagccag attatcaatt tcataaagaa ggctgctcgg aagaagaatc
    10501 aaccccattc aatgagccga agcaatttta caaaaggggg aaaagtaggg gcaaatctca
    10561 acgctatcaa tgtaaggcgt gtaagaagtt cactaatgtt ctaccaaaaa gagaagaaac
    10621 cactacttat caccaacaaa agaacaccat tcttccaatg ttcgctaaga tggttgttgg
    10681 aagggtttca gtcagtcgta cttgtgatat tttaggaatt ggagttggaa cctactatca
    10741 taagctggag tggctgtata ggcgttgttt ggagttttta gaacgatatg agacacaacc
    10801 ccttcaaaca aagaaattta acgagatgtg gcttaatacg gacaagatgc actactacct
    10861 aaataacgtc cgtaagaaag gacaaggttc aaagaaatat acggggtttg aagaccttaa
    10921 tatgcaaacg tatatagttg tatctgctga ggtcttatca agatatgtgt tccgttctga
    10981 tgttgcctat gattggaata tttcaatgga tgaattaaat gaagatacaa gaaaattcaa
    11041 agaagaccat ctcaatactt ttagtcgtaa aaatgatagg ttagattggt cttattatcc
    11101 acaagaacca tccgctaacg atagcgaaaa taggaatgct tatcttcacg aattagggaa
    11161 aattactaat aggagtagat ttgtcgatgg tttaaatgtt gatgctcctt atactacaac
    11221 tgctcactat tggttaatta aacaaatggt taatgccgac gaatggcgaa tgattagtga
    11281 tgatgacttc tctattagaa acgcctttta tagagtattc acgaaagaat taaggttgag
    11341 cgatgcacat cattttattt gccaagttaa caagacgaaa agcagaaaac aatgcttaaa
    11401 agaattcgga caagcaaaag cagaactgct cgattgggga gatatcagag gattcaaaac
    11461 aaagtttcta cggactttgg catctcatta tctaactgaa ttactaacaa gtcatcaatt
    11521 ccacgaagag gctattagta aagatggaga aagatatcgc aaatatgccg ataaccctat
    11581 caaacatcca ttagccacta aggataaagg attttattca gttgattgta gaacggattt
    11641 atctgctctg gaaccaaacg aaatcgcaaa gatgctatta aatgttaatg accattcgac
    11701 caatagcttt attcaacaga taagacgtta tatatcatct cttgaaagac cattaacaac
    11761 agctcgtggt gataagaaga gctatattta cgctaacttc aatccgaagt atgctcaatt
    11821 tgcgattaca attctaagaa cttactacaa cttttgtcgc ccttttaaat cagcggataa
    11881 aaaggttttg acacctgctc aaagattagg gattacagat aaacaatttg attggaaaga
    11941 tataatttat ttcaaatgac aaaaagagct tggagtaatc ccaagctctt gtgctttatc
    12001 tttactgttc ttcttcaatt aacgcacccg ttaactcaat aagttttttc tttcatatac
    12061 atgaaaatag tagttatacg gatttttatc attctttatc cctttttggg caaatacctc
    12121 attccattcc tcataattca cttctggaaa aaaagtatct ccttcgaatt catgatgtat
    12181 ttttgtgatg tacattttct caacataagg gaaaaacaaa ttataaatct gttctcctcc
    12241 gaaaataaaa atttcttctt cgtttttaca taactcaaaa acatcttcta ttgaatgaac
    12301 aatttcacaa ccattaaagg taaacccctt atctctcgtc agaataatat ttcttctgtc
    12361 aggtaaggct cttccgattg attcaaggtt cttcctacct aatattatcg gatgtccctt
    12421 tgtagtattt ttaacatatt cccagtcctt gggaatcctc caaggaatgt cattctcttt
    12481 gccaatcact ctattcttat ccatcgcagc aatcaaagaa actttcattt ataacacagc
    12541 ctttttaact aat
//
LOCUS       DAZUMB010000080        12205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000080 DAZUMB010000000
VERSION     DAZUMB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.082"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..216)
                     /locus_tag="ACG80L_001915"
     CDS             complement(37..216)
                     /locus_tag="ACG80L_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024135.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(232..432)
                     /locus_tag="ACG80L_001916"
     CDS             complement(232..432)
                     /locus_tag="ACG80L_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024136.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(597..1304)
                     /locus_tag="ACG80L_001917"
     CDS             complement(597..1304)
                     /locus_tag="ACG80L_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024137.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="ACG80L_001918"
     CDS             complement(1297..1734)
                     /locus_tag="ACG80L_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024138.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="ACG80L_001919"
     CDS             complement(1749..2000)
                     /locus_tag="ACG80L_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024139.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="ACG80L_001920"
     CDS             complement(2000..2206)
                     /locus_tag="ACG80L_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS5024140.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="ACG80L_001921"
     CDS             complement(2643..2903)
                     /locus_tag="ACG80L_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024141.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3077..3181
                     /locus_tag="ACG80L_001922"
     CDS             3077..3181
                     /locus_tag="ACG80L_001922"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS5024142.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACG80L_001923"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACG80L_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5024143.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="ACG80L_001924"
     CDS             complement(3810..4709)
                     /locus_tag="ACG80L_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS5024144.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="ACG80L_001925"
     CDS             complement(4722..5315)
                     /locus_tag="ACG80L_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS5024145.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="ACG80L_001926"
     CDS             complement(5391..5540)
                     /locus_tag="ACG80L_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024146.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="ACG80L_001927"
     CDS             complement(5556..7760)
                     /locus_tag="ACG80L_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS5024147.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="ACG80L_001928"
     CDS             complement(7771..9231)
                     /locus_tag="ACG80L_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS5024148.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="ACG80L_001929"
     CDS             complement(9292..11664)
                     /locus_tag="ACG80L_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS5024149.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            12074..>12205
                     /locus_tag="ACG80L_001930"
     CDS             12074..>12205
                     /locus_tag="ACG80L_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5024150.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         3040 a         2531 c         2053 g         4581 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca tttgtctaat
    11701 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    11761 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatggaaa ggatccaacg
    11821 ggaatttgct atttcctcct gtagcgtttg tctaccctgt ttagtctgtt acccgaacca
    11881 ctgttatcac taccaactca ttagctgttt cgataatgga ctagactttc aaatagtagt
    11941 ttggaaggca gcttctagct cttttatcac cgtattctat taggcgaggt tgtggctata
    12001 cagtgagttt ctgggaaatc ccacagtatc gaagcatttt ccaaaattta tatcgaaagg
    12061 aggtccttct tccatgaaat tatttgtcgg tattgacgtt agctctgaaa aactagatgt
    12121 ttgtttttta acagacgaca atcaattgtc tatcttatct gaaatctctg ttgccaatga
    12181 catcgaaggt gcttcattca tacga
//
LOCUS       DAZUMB010000081        12175 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000081 DAZUMB010000000
VERSION     DAZUMB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.083"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..301)
                     /locus_tag="ACG80L_001931"
     CDS             complement(137..301)
                     /locus_tag="ACG80L_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024151.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            596..1924
                     /locus_tag="ACG80L_001932"
     CDS             596..1924
                     /locus_tag="ACG80L_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS5024152.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2126..2959
                     /locus_tag="ACG80L_001933"
     CDS             2126..2959
                     /locus_tag="ACG80L_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS5024153.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2975..3712
                     /locus_tag="ACG80L_001934"
     CDS             2975..3712
                     /locus_tag="ACG80L_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS5024154.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3867..4835)
                     /locus_tag="ACG80L_001935"
     CDS             complement(3867..4835)
                     /locus_tag="ACG80L_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS5024155.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5067..5900
                     /locus_tag="ACG80L_001936"
     CDS             5067..5900
                     /locus_tag="ACG80L_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS5024156.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5934..6773
                     /locus_tag="ACG80L_001937"
     CDS             5934..6773
                     /locus_tag="ACG80L_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5024157.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6799..7359
                     /locus_tag="ACG80L_001938"
     CDS             6799..7359
                     /locus_tag="ACG80L_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5024158.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7558..9279
                     /locus_tag="ACG80L_001939"
     CDS             7558..9279
                     /locus_tag="ACG80L_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS5024159.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9444..10586
                     /locus_tag="ACG80L_001940"
     CDS             9444..10586
                     /locus_tag="ACG80L_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5024160.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10602..11813
                     /gene="thiI"
                     /locus_tag="ACG80L_001941"
     CDS             10602..11813
                     /gene="thiI"
                     /locus_tag="ACG80L_001941"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS5024161.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4151 a         2042 c         2527 g         3455 t
ORIGIN      
        1 aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt tcttttatta
       61 ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac gaaagagtag
      121 agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa taaatcagca
      181 ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc ccaaagttat
      241 ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc aggtttttca
      301 ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt attttgaaag
      361 ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc attttcgtta
      421 tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac atttttcttc
      481 atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga gattgtattt
      541 ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg attggatgaa
      601 agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa atatcaaaca
      661 gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg aaaatgtttc
      721 agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga aagtggctaa
      781 tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc gtcataagat
      841 gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt ttttctggga
      901 taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa aagaggcaat
      961 cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg gccaatggga
     1021 tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga tgccggaaag
     1081 tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac tgcgtaaaga
     1141 tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta ttgaagaaaa
     1201 gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag gaacacctcc
     1261 aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg accgaggact
     1321 gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt atgttagcgt
     1381 gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag agatgctagg
     1441 aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa ctttcaaaaa
     1501 attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg atgtaggtca
     1561 atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg atgatctatt
     1621 tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa gtctgatgac
     1681 ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa aatggaaagt
     1741 cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga gtgatgcctg
     1801 gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga aagagcaatt
     1861 aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg gtgctttagc
     1921 atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg cttaagccag
     1981 ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta taattctaaa
     2041 taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac gaagtagaat
     2101 aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg atctgaaatt
     2161 aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc gtataaaaac
     2221 cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat tttttgaagc
     2281 agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata cgtatatgct
     2341 aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg gaaaagggga
     2401 tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc cacaatttgg
     2461 ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg aagaagattg
     2521 gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag aagaaacgaa
     2581 tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg tattgaaatc
     2641 ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga gtggtaatgt
     2701 gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt atgggaatcg
     2761 ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat ttttagatgc
     2821 ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga tttaccagtt
     2881 atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc aagtagatca
     2941 attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata ccaaaaagca
     3001 tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt cgatgttagg
     3061 agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca gtgaattcgt
     3121 caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta atcgtctccc
     3181 tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata agtttttctg
     3241 ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa tagaatctgt
     3301 tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag agatcattgc
     3361 tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg aaaagattga
     3421 ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta atcagcatgt
     3481 taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact ttttgactac
     3541 gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg ggaatgagat
     3601 cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac atgaaatccg
     3661 aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat aaaaaagagg
     3721 ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat tgtttctcaa
     3781 atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag cctctctttt
     3841 taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa ttcttcaccc
     3901 gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact attaatactg
     3961 ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata atcataaaca
     4021 ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt ttggacaaga
     4081 agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa agcttcgatt
     4141 accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg catcccagca
     4201 tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg ctttgtttct
     4261 aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg acccaatgta
     4321 ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg tgcagaatgg
     4381 atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca acttgcgaca
     4441 cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact tgaacgcatt
     4501 gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa aaaagcagtt
     4561 tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga tgcataagcg
     4621 gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg tgagggaaga
     4681 ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc tgcatctaat
     4741 aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt catggcgatc
     4801 gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt acgaataatt
     4861 ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat cagtgaagaa
     4921 taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa aagactcaac
     4981 cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa agcagccaag
     5041 gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg tttaaagaac
     5101 atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg tttctaccct
     5161 tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt gagaattgga
     5221 taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga atcagcggtc
     5281 aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat tttttagatg
     5341 ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa ttcccctcat
     5401 cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag tggtttgagg
     5461 gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa tttcttgaac
     5521 gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat gagccacagt
     5581 tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt gctctttttc
     5641 gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg cgtaaaaaaa
     5701 gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa gtcaaagtag
     5761 ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt ggtgatgcgg
     5821 ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga ctcaatccaa
     5881 cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag tctatgatca
     5941 aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa gcgttggaat
     6001 caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt gtagcaacgg
     6061 ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat atgtatgtcg
     6121 tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac ccttttgaac
     6181 aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa atcgtttttg
     6241 gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg atttttatca
     6301 agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa aaaatcctcc
     6361 aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata ttaaaagaac
     6421 caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta gaacatcgct
     6481 ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc ccaaaaggtg
     6541 gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa atggaagaaa
     6601 ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta ggaattggag
     6661 tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg actcaatcaa
     6721 atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat tagtcaatga
     6781 aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga aacattccac
     6841 aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa tgaagctcat
     6901 acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac ttcacctaat
     6961 aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa agcagaagaa
     7021 aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt agatgcactg
     7081 acagatatcc tttactttac ttatggttct ttttcattac taggaatagt gcctgatcga
     7141 atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg aaaacctcat
     7201 tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga atatgctcca
     7261 gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc taagaagaaa
     7321 tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca ctatttttgt
     7381 tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg catgaaaata
     7441 gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt ggatttatgc
     7501 caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga accataaatg
     7561 ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat tgtcgtcttt
     7621 ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt agaaaaacgc
     7681 aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa aaaaatgcat
     7741 ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa tgacatatct
     7801 acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct caatgaaagt
     7861 tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat ggaccaaatg
     7921 gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac ggaagaacgc
     7981 aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga aaaagtcaga
     8041 gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat caaagtgatc
     8101 gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt tgaagcccgc
     8161 gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat gaaaaaaatt
     8221 ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga aatcagtgat
     8281 acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct tgctgaagat
     8341 atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa aacagaagtt
     8401 gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt atatgatatc
     8461 ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac gattgcagag
     8521 tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga tcacacatct
     8581 caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca aaccgaagtg
     8641 gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca cgaaattcct
     8701 tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga tatcgaaaag
     8761 caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa agaagcgcaa
     8821 gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt agaaaaacaa
     8881 cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac tgaccgtatt
     8941 gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt caatcgttta
     9001 gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga tttagtagat
     9061 gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca tacacacgaa
     9121 gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata tcactatcaa
     9181 gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc tttcaaacgc
     9241 atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt tagtaaacca
     9301 gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt gaaaattgaa
     9361 acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata atagaaaaga
     9421 atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca accccaatct
     9481 ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg aatccatcaa
     9541 gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga aagcagatcg
     9601 ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga acagaaggag
     9661 ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat catatgatca
     9721 tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa gaattaggaa
     9781 tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac gaattgaaag
     9841 aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat gaagtaggat
     9901 cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa attcatttcc
     9961 atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg acagaaagag
    10021 ttgattttgc aacattttca gctcataaat tccatggccc acggggaact ggtttcattt
    10081 attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa gaaagaaatc
    10141 agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct ttacgtttat
    10201 cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta gctctaatca
    10261 atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac tatgcaccgc
    10321 atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat gccttagaag
    10381 aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat atggcaagca
    10441 gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt cgaatcagct
    10501 tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc aagcagcttt
    10561 ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac agtgaaatta
    10621 tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt atcatgcaac
    10681 tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc catgctgatc
    10741 gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt ccaaaactag
    10801 caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa gatgtcgaag
    10861 ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa gaaacattta
    10921 aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa ttgaatcaaa
    10981 cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg cgtcaaccag
    11041 atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat gaaacgatca
    11101 agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg ctttctgggg
    11161 ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa attgaagctg
    11221 ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca aaagatctaa
    11281 cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca tttaccgaga
    11341 ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt actcgtcgaa
    11401 tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc attttgaatg
    11461 gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct atcaatgaag
    11521 taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa atcattgaag
    11581 tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat tgctgtacga
    11641 tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat caatatgaag
    11701 agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag atggaagaga
    11761 tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta taagaggtta
    11821 tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag ctccatgtat
    11881 tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta tcaagaagtt
    11941 atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag agatcaagct
    12001 acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt tgtgacagaa
    12061 gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa tttttgtgaa
    12121 atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga ggttg
//
LOCUS       DAZUMB010000082        12107 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000082 DAZUMB010000000
VERSION     DAZUMB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.084"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..694
                     /locus_tag="ACG80L_001942"
     CDS             <1..694
                     /locus_tag="ACG80L_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS5024162.1"
                     /translation="LVTVAKRPLDRIDQEAVFCQELAIQGTLLPLTETYLKEYFPELH
                     ATEAFTVLQAFFLSRVINHLIAPIQNKIMAETIEKMKQDSKEIYAKNNSFLVASLSEL
                     KITDTYLPDIATSLTLLVRRLKERYLQDRKKIIFLLEGDLYVCQSIRSRVLHYFGKRH
                     DLFFMTIQALSDEFLAQEDIDLVVTNYSDYLSEYTVNTDYLLLKTVPDSQDWRLLFQK
                     IEPQLSTLIGLN"
     gene            complement(752..1747)
                     /locus_tag="ACG80L_001943"
                     /pseudo
     CDS             complement(752..1747)
                     /locus_tag="ACG80L_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558311.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1860..3062
                     /locus_tag="ACG80L_001944"
     CDS             1860..3062
                     /locus_tag="ACG80L_001944"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HFS5024163.1"
                     /translation="MLVKYKVNDAGSRPVKIKPRELYRASHLDSCIYRYYAECLNKDY
                     EKVAEIRRIGDNVVAYRVGKGKSNIDYAAEAIDFIKRSNDGCYIFLGDFEKFFDTLDH
                     EYLKCKLKEVLSMSTLDEDWFKIFKTITSFSWVEQKDLNDLFGSEKEQYKNGLKSYFD
                     TWEEFRNFKADGHVCKNTNSFGIPQGTPISALLSNIYMIDFDDWLNKLSSKYGGKYLR
                     YADDFILILPFSTTSKDIFLEIIREILQYASEVTKLKIERNKTEKYLFTNDSVYLFES
                     NKNSTSSLVKSKLDYLGFVFDGSQVKMRQKSVSKFYRKADKAIRAAREKSRIKKSSYL
                     IGKRHIYRYFFDVGVRDSKGSKFGNFISYARRCQMKFDQISPLTENLMMEQIKNRRKK
                     LLKKMYKN"
     gene            complement(3227..3460)
                     /locus_tag="ACG80L_001945"
     CDS             complement(3227..3460)
                     /locus_tag="ACG80L_001945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024164.1"
                     /translation="MSQLNQTYENYLQQPEALSFTEMTTIYQEILKNADMNDKDFIEI
                     WEELMGAAIRYTVILGHHAYSYKKNKNNVQEVM"
     gene            complement(3719..3913)
                     /locus_tag="ACG80L_001946"
                     /pseudo
     CDS             complement(3719..3913)
                     /locus_tag="ACG80L_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367047.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3974..5487)
                     /locus_tag="ACG80L_001947"
     CDS             complement(join(3974..5326,5329..5487))
                     /locus_tag="ACG80L_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5024165.1"
                     /translation="MYTYEERKKAVDLYFKYDQALAATTKKLGYPSVGALRQWIREFQ
                     SSDKLHKNIRTSWKYTLAQKEVAVNHYLDYGQCYARTIRKFGYPNKTTLRKWLEEYAP
                     STRKVRQNSIHYTKQEKEAAVLALCTRSLSAKSIANDFGVTRQALYLWKNKLLGKGAT
                     KPLKSMNVTPEIKLLKEERDILQREVYKLRLEKEILEQASDLIKKEPGIDPIILTNKE
                     KTQVIDALCSKYSVIELLRILDLPKSSYYYHRKQLAIPDKYLKERSLIVQLFHDNKGR
                     YGYRRIYCSLKLLGITLSEKVVRRIMTEEKLKVKSLRLRKYNSYGGEITPAVPNLLKR
                     DFKADQPNKKWLTDITEFHIPSGKVYLSPMIDCFDGAIVSWTISTKPNADMVNSMLEN
                     AIATLNAKDSPIVHSDRGAHYRWPGWIKRMKEACLVRSMSKKGYSPDNSACEGFFGRL
                     KNEFFYGQDWKSTSIDQFITDLDNYILWYNKKRIKCSLGGLSPLDYRKKLGIL"
     gene            complement(5810..6634)
                     /locus_tag="ACG80L_001948"
     CDS             complement(5810..6634)
                     /locus_tag="ACG80L_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5024166.1"
                     /translation="MEIKVLFENSRINLNFKSGYGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTEIAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            6977..7393
                     /locus_tag="ACG80L_001949"
     CDS             6977..7393
                     /locus_tag="ACG80L_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5024167.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            7390..7860
                     /locus_tag="ACG80L_001950"
     CDS             7390..7860
                     /locus_tag="ACG80L_001950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5024168.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            7874..8647
                     /locus_tag="ACG80L_001951"
     CDS             7874..8647
                     /locus_tag="ACG80L_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5024169.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            8661..9473
                     /locus_tag="ACG80L_001952"
     CDS             8661..9473
                     /locus_tag="ACG80L_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5024170.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            9527..10492
                     /locus_tag="ACG80L_001953"
     CDS             9527..10492
                     /locus_tag="ACG80L_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5024171.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            10629..>12107
                     /locus_tag="ACG80L_001954"
     CDS             10629..>12107
                     /locus_tag="ACG80L_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5024172.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQE"
BASE COUNT         3950 a         1957 c         2274 g         3926 t
ORIGIN      
        1 tttagtaacg gttgctaagc ggccgttgga tcggattgat caggaggcag tcttctgtca
       61 ggagttggcg atccaaggga ctttacttcc attaacagaa acgtatttaa aggaatattt
      121 tccagagttg cacgcaacag aagcatttac tgtcttgcaa gcattctttc tttcccgagt
      181 aatcaatcac ctaatcgccc ctattcaaaa taagattatg gcagaaacga ttgagaaaat
      241 gaaacaagat agtaaagaaa tttatgcgaa aaataattcg tttctggttg cttctttatc
      301 ggaattaaaa ataaccgaca cttacttgcc tgatattgct acgagtttaa cgttattagt
      361 cagaagattg aaagaacgtt atctacaaga tagaaagaag attatctttc tgcttgaagg
      421 ggatttgtat gtctgtcaaa gtattcgttc aagagttctt cactacttcg gcaaacgtca
      481 tgacctattc tttatgacga ttcaagctct aagtgatgag ttcttagcgc aagaagatat
      541 tgatttagtc gtaacgaatt acagcgatta tttgtctgag tacacagtaa atacagatta
      601 tctactatta aaaaccgtac cagatagcca agattggcga ttgctattcc aaaaaattga
      661 accacaactg agtacgttga ttggtctgaa ttaacttgaa gcctatttaa cttttttcgt
      721 taagtaggct ctttttttgc gtcttgaatt gtcaaatcaa gtgcaacact ttattaaaga
      781 agacttcata aggtgttttc caacctagac actttctagg tctgctattt agtttttcaa
      841 ccatccgata aatcgttgcg tcatctaccg tgtccatctc ttttgatttc ggtaagtact
      901 ctcgaatgag tccattcgta ttttcatttg ttcctcgttg ccaaggggca tgcggatctg
      961 gaaaataaaa gggcacatta ttcaatgctt ctgtaaccgc gaaatgattg gagaattctt
     1021 ttcctctatc tggagtgatc gttttgacca catcattggg gatactgctt aaaagttcaa
     1081 tcattttttc gctcacgtaa gcagctttct ttcttggtac ctttccagct aaaaggaatc
     1141 gcgattttcg atctgtcaac gtcactaaac aagcaccacc agtttttcca gctacagtat
     1201 cggcttccca atgtccaaat tcacttctat cgtctgcaga ttttggacga tcatgaatcg
     1261 aatgcgtaat gcgtattttt ccacgagcct cgacatgatt ttttgtatgt cttgtttttc
     1321 ctcgatggcg taatgaacgg atcagccctc tattgccact cgagagcggt tctgtatcga
     1381 aaataccgtt gtaaatggca cggtagaccg tgataaaact gattgagaag tccacctgtt
     1441 caagcttcaa acgattagag atttcctctg gcgaccattg aaattctaca aataaatgac
     1501 gaacgattga ccaaattcgc ggatcattta gaatagaatg acgccctcag tcctttctac
     1561 gagaagcata ctttttgtct gctttggaag gtgagtaggt tttctccttg tttcttctca
     1621 attcacgtga aatggtagac ggactacgat gaatgtcatt gccgattttt ctaatagaaa
     1681 caccttcttc atgaagtaga aagattcttt ctcgttcgag tatggtaaga tgatggtact
     1741 ggctcataat tgtcttcctc ctagtagtgt tctcgacaaa cctattctag ttggatattg
     1801 attatgagtc attttttagg tgttgcactt aaagtgtaaa ttcaaaacac aaaaaaaagg
     1861 tgttagtaaa atataaggtt aacgacgcag ggagcaggcc tgtcaaaatc aagccgcggg
     1921 aactttacag agcttcacat ttagattctt gcatctacag atactacgca gaatgtctaa
     1981 acaaagatta tgaaaaagta gcagaaataa gaagaattgg tgataatgtt gttgcttata
     2041 gggttggaaa aggtaaatca aacatagact atgcggctga agcaatagat tttatcaaaa
     2101 gaagcaatga tggctgttat atttttttag gagattttga aaaatttttt gatactttgg
     2161 atcatgaata tttaaagtgc aaattaaaag aagtcttaag tatgagcaca ttggatgaag
     2221 attggtttaa aatttttaaa acaataacta gtttttcttg ggttgaacaa aaagatttaa
     2281 atgatttatt cggttcagaa aaagaacaat ataagaatgg tttaaaatct tattttgata
     2341 cgtgggaaga atttaggaat tttaaagcag atggtcatgt atgtaaaaat actaacagtt
     2401 ttggaattcc tcaaggaacc ccaataagtg ctttgctatc aaatatatat atgatagatt
     2461 ttgacgattg gctaaataaa ttatcatcca aatatggtgg gaaatattta agatatgcgg
     2521 atgatttcat tttgatactg ccctttagta caacctctaa ggacattttt cttgaaatta
     2581 tccgagagat tctgcaatac gcatccgagg tcactaagtt aaaaattgag agaaataaaa
     2641 cagagaagta tctttttaca aatgattcag tatatttgtt tgagtcaaat aaaaatagta
     2701 catcaagtct agtaaaatca aaattggatt acttgggatt tgtatttgat ggaagccaag
     2761 taaagatgag acaaaaaagt gtttcaaaat tttatagaaa agctgataaa gcgatcagag
     2821 ctgctagaga aaaaagtcga attaagaaat cgagttatct aattggtaaa aggcatattt
     2881 atagatattt ttttgatgtg ggtgtaaggg atagtaaagg gagtaaattt ggaaacttca
     2941 tttcctatgc tagaagatgt caaatgaaat ttgatcaaat atctcctctg acggagaatt
     3001 taatgatgga acaaataaaa aacagacgta aaaagctatt aaagaaaatg tataagaact
     3061 gataatcaaa tgatatgctc ccatcatagt agacgttaga aaaaaatatt gataatctac
     3121 tatgatgaga atttatcaaa tcgtgacttt catatctatc gtccattaac cgctgaaaca
     3181 aacaccaaat aattcgcgaa atcgccaatc cgttgtcggt ggtcattcac atcacttctt
     3241 gtacgttgtt cttgttcttc ttgtaagaat aagcgtgatg tccaagaatg actgtataac
     3301 gaatagcagc acccatcaac tcttcccaaa tttcgataaa atccttatca ttcatatcag
     3361 cattttttaa tatttcttga tagatagtgg tcatttcagt aaaagatagc gcttctggtt
     3421 gttgtaaata attttcgtat gtttgattca attgggacat taaatcactc cttgaaatgt
     3481 ttttgtttta actagtttac tacaagaaat aaaagttagt gcatcaaaag aaaaacagga
     3541 aagcatacca ttttggtagt tttttctcgt tgagttggta taaatttgat gagacaaata
     3601 ggctagtatc actttattca ttgatactag cctattttgt atccaaaatt tatgaaaatg
     3661 attaaaacaa atctttttct tgctcaatga attgagcaaa tttatcagca cttcgttctt
     3721 atcgttctgg ataaaaatag ttgtagactt gttttgtcat ttttgaatct ttatgtccaa
     3781 cacgatacat aatatcctat agtttcatgt ctgccattgc gcacaaggaa acgtgagttt
     3841 ttctaaattc atgaagagtt atccgttttt caaggtgata tttattagcg atcatgtcat
     3901 accaatcatt tggtgtcaag ggcatcttaa aattgtaggt ttgggggtgc ggacgttttc
     3961 ctgcaccaaa attttataaa atacctagtt tcttacgata atctaatgga cttaatccgc
     4021 ccaatgaaca cttgattcgc tttttgttgt accaaagaat gtagttatcc aaatcagtga
     4081 taaattgatc aatagatgtc gacttccaat cctgcccata aaagaattca ttcttgaggc
     4141 gcccaaaaaa gccttcacaa gcagaattat caggagaata tccttttttt gacattgaac
     4201 ggactaaaca ggcttctttc attcgtttga tccaacctgg ccaacggtaa tgtgcgccac
     4261 gatcagaatg aacaattgga gaatcctttg catttaatgt ggctatagcg ttctctaaca
     4321 ttgaattgac catatctgca ttgggttttg tgctaattgt ccaactaacg atagctccat
     4381 caaaacaatc aatcattgga gaaagataga ccttcccaga gggaatatga aattctgtaa
     4441 tatcagtgag ccatttcttg tttggttggt cagctttaaa atcacgtttt aaaaggttcg
     4501 gaacagcagg agtaatttct cctccataag aattgtactt tcttaatcga agtgatttca
     4561 cctttaattt ctcttcagtc ataatgcgac gtaccacttt ttcagataaa gtaattccta
     4621 ataacttaag cgaacaataa atacgacgat agccatatct tcctttattg tcatgaaata
     4681 attgcacaat taaggagcgt tcttttaaat atttgtctgg gatagctaat tgtttcctgt
     4741 gatagtaata gctacttttg ggcaaatcaa gaattcttaa tagctcaatt acgctatatt
     4801 tggagcataa ggcatcaatc acttgtgttt tctctttgtt tgtcaatatg attggatcga
     4861 tgccaggctc tttttttatt aaatcagacg cctgttctaa aatttctttt tctaagcgca
     4921 gtttgtatac ttcacgttga aggatatctc gttcctcttt caacaatttg atttcaggag
     4981 tgacattcat tgatttcaaa ggtttagtag ctccttttcc aagcagcttg tttttccata
     5041 agtacaatgc ttgtctggtt acgccaaaat catttgcgat tgatttagca gagagtgaac
     5101 gagtgcacag ggctaggaca gcagcttctt tttcttgttt tgtatagtgt atcgaatttt
     5161 gacgaacttt acgagtagat ggagcgtatt cttccaacca ttttcttaaa gtagttttat
     5221 ttggatagcc aaactttcga attgtacgtg cataacattg cccataatct aaataatgat
     5281 tcacagcaac ttctttttga gcaagcgtat atttccaaga agtacgttga tatttttgtg
     5341 cagcttatct gaagattgaa attctctaat ccattgtctg agggcaccaa cagaaggata
     5401 tccaagtttt ttagttgtag cagccaatgc ttgatcatat ttaaaatata aatcaacagc
     5461 ttttttccgt tcttcatatg tgtacatgag acgtcctcca aacttatttc agtccaagtt
     5521 tttgtccgca tgcccttgta tgtactttta ttctgccata aacagcttaa tgaatgtcca
     5581 cttgaaaaag tgaataaacg aaatagatga ctcgatttaa taaggaagta aacccacaca
     5641 gaaatatagg tgattttcct tgaatgacca agatattgaa agttagtgaa aaaacacaaa
     5701 aaggtagccc tattcacaga aggtctgcct tttgtgttat cgccagcaaa cattagaggg
     5761 gatatttcat atagcttttg tcctctcgac agtcctatca gtttctcttt taacttatct
     5821 ctaagtgctg tccagtttta atagcttcta gcttgtttcc taactgtgtt tttaatatgt
     5881 tgaaggcttt tgaacctgtg cagtggcaag taaggatttt ttctactcgt gagtcattca
     5941 atttatttcc aattttcatt acctcttgct caggtagacc taaattattt atataaggta
     6001 tgccaatcat atggaagcca ccgattaggt attttatggg tttagtcgga aatgtttcaa
     6061 gagcaatttc catcatatcg ataatgccat gatgactaca gccagtaaag acaatcagac
     6121 cgtcttcttg ctcaattacg accaataatt catctgaaaa atcatcataa acaaatttat
     6181 catccatctt tttgtataaa ttcttggtgg tcgagtttgc tttgcctagg gggatgatat
     6241 gaaagctcgg agcaatttct gttttcgttt ttattgtatg taaattctca tgcattttaa
     6301 ggatttcatt tttaggaata ccaacataca gaaacgtggt taaaatcttt aaataatggg
     6361 gtaaagttac accttcagct gtataaatag gggcgtttcc tgattttaaa tatcttaacc
     6421 cgccggtgtg gtcagaatga ccgtgtgaaa taatcactgc ctccagatca ttcaaagttt
     6481 cccccatttt agtcgcatta tagactaatt ttttattagc gccagcatcg aagagatatt
     6541 tcttccctga gtagttaatt aagagagata acccatatcc acttttaaag ttcaaattta
     6601 ttcttgagtt ttcaaataaa accttgattt ccactataac atctcctttc acatgaacgt
     6661 gtattcatat attgtagcac attaagttct attttttgtt gtaattaccg aaaaaaatat
     6721 taatggacgc tttttacgct ttatttcgta tgtggaagaa aatttcttag gatgtggcgg
     6781 gggattgggg caaagctaaa ttccaattac agtgatcatt taattagaca cctttaaata
     6841 cccacacttt catcaacact ataatttagg gaaagaaaag tgttggcatg atatagaaaa
     6901 acctttaatt aacagcgttt cctaaaattg gcacgcatct tgcttataga aaagcgtaag
     6961 gataggagga atgaaaatgg caactataat tcttgccagc catggggaat ttgctcaagg
     7021 attaaagcaa acagcgacaa tgattattgg agatagtgta ccaattcatg ccttgtcagc
     7081 atttagagat gaagatgaat ctattttggt acaaataaaa aaacttttag ccacaataga
     7141 tatagaagaa acctacattt taactgatat tctaggtggt agtgtgaata atgatatgct
     7201 cactattatt aaagagtatg agggattgca attaattact ggaatgaact tacctttagt
     7261 tatttctatt gcaacctata atggaaaaat tggggaaagt gagctagaaa agcttattag
     7321 agagagtcaa caatcagtga ttgattgtag aaaattatta gtagaacaac ttactggagg
     7381 agatgaccta tgattaaatt agtacgtatt gaccatcgtt tgttgcacgg acaggtagta
     7441 ttttcttgga gtaaatcatt aaatatcaat cgtattatta ttgccaataa agaggcagcg
     7501 aatgatgaat tcaaaaaaat gtctttgaat ttatctaaac cgactggcat taaattaaca
     7561 attttttcag ttgaacagtt actagaaaaa atgcctaaaa tcgaaacact taacgaaaat
     7621 atcatgctta tttttggtaa tacaaaagaa attcgtcaat tttgtgaagc ctatcctaaa
     7681 gtacaagaaa tcaactatgg agggataatc aaaaaagaag gttccaagca atttagtaat
     7741 gccattttct tgaatgaatc tgagcaagaa gatgccaaaa aattaaaagc tcttggcatt
     7801 aaacaattta ttcaacaggt accaacttct aaaaaagaag atttgaatac aatgttataa
     7861 aggaggaatg aaaatgctcg ttcagtcaat tggtttaggg ttaatcggtg ttttttgtat
     7921 tttagactca aggcttttag gaagaatgaa ttttgaacga ccgttgattg tcagtacgct
     7981 tgttggtttg gtacttggag acatggaaaa aggattaatt gttggagcct ctttggaatt
     8041 gatgtcctta ggattagtaa atatcggagc tgctgcacca cctgatatga atatggcatc
     8101 aattattgct acagcctttg ccattttatc aaatgcgaat gcagaagcag cattaacgat
     8161 tgccattcca atttcagtgt taggtcaaat actaggtatc ttaatgcgga cgattttgtc
     8221 aaatttgact cacgttgcgg atcgctttat cgaacaagga aaatttagcc aagcaaggag
     8281 aattcatata ctttatggct cgggattata tgctctcatg tattttattc ctatttttct
     8341 agcaatttat tttggaacga atcttgtttc tcagattgtt gatgcgattc ctgagtggct
     8401 aacaaatggc ttaacggttg caagtaaaat tttaccagca tatgggtttg cgttgttatt
     8461 acaaacgatg ttaacgaaaa aaatggtgcc atttttatta cttggatttt taattactgc
     8521 ttattctgga ttgggaatca ttggtattgc agccttcgca tgtattgttg cgtttgtcat
     8581 ggttcaagtt caaggtaaaa actcaaatgg taaccaaaat gagccagatc cattagatga
     8641 cttataagga ggtagattaa gtgaaagaga aagatcaaaa tgcatggaaa tatcggcagt
     8701 tcttatggcg ttcatgggca attcaagcct catggaacta tgaacgacaa atgaacatgg
     8761 gttttatgta cggaattgca ccaattctag ataacattta caagaatcca gaagatgaag
     8821 caatgaagaa agaagcttac gagcggcata tgatgtacta caactgtacc cctcaaacaa
     8881 gtgcatttgt gttagggtta actgcttcta tggaggagca atactacgaa gaccaagaaa
     8941 actttaatcc ggaatcaatc agtgcagtga agacttctct gatgggaccg ctatctggtg
     9001 ttggtgattc tttctttcaa ggaacgattc gggtattagc ctttggacta ggaattaatt
     9061 tagcccaaca aggtagtatt ttaggtccaa ttctagcaat gttaatttcc tttgtacctt
     9121 cctttttagt tacttactat ggtgggaaaa ttggctataa catggggaat aaatacttgt
     9181 ctaaactgta tcaagaaggt ttgatggata aagtaatgta tgtttgctcc attgtggggt
     9241 tgatggtcat tggttcgatg attgccagca tgatcgggat tacaacacca attcaaatgg
     9301 gtaaagcttt tgtcttacaa gatgtattag atagtattat gccacaattt ttaccattag
     9361 gattaacctt cttcatgtac tggttattgc agaaaaaggt caaaacagga tggatgttgg
     9421 caatctgttt agtcggagga attattttta gtttgttagg tatcttacaa taagggaata
     9481 accaataaat ttggttgaat aatataaata aggaggaatt caattgatga attcaaaaga
     9541 aattgttaaa gaaatcgtag cgaaaaaaga ggtaaaatca atcgtatttg ttggctgtgg
     9601 cgcttcaaaa gcggaccttt atcctgcaaa gtattttcta gaccaaaatg cgaagcagtt
     9661 acggattagt cattacacag ccagcgaatt taactatgca acaccagcat ctgtagatga
     9721 gtcaactgtc gttatttcag cttctttagg aggtacgact ccagaaacag ttgaagctaa
     9781 cgcagtagct aaagaaaaag gtgctacagt tattagtcta acacgaagca ttgactcacc
     9841 gttaacaaaa gatgcagatt atgtaattta tcatcgattt gcagagaact atgctgctaa
     9901 attagaaaaa acaggctact gtttagaatt ggcagtagaa cttttacatc aagtagaagg
     9961 ttatgaccat tatgaagcaa tgcaagaagg atttgctact atctatgatc ttgcccaaaa
    10021 aagtgcagaa tcagctcgta aagatgcgaa gaaatttgct gaacgcttta aagatgaaaa
    10081 agttatttat gtaatgagta gtggtgctac acatgaagta gcatactcaa catcaatttg
    10141 cttgatgatg gaaatgcaat ggattaattc tggtaccttc cattcaggag aatttttcca
    10201 cggaccattc gaaattgtgg ataaagacgt accgtttata ttattaatga atgatggaaa
    10261 aactagaaat atagatgctc gagcattaac tttccttgaa cgctttgata cgaaaacaga
    10321 agttgtagat gccttggatt atggcttgtc ttcacatatt gctaaagaag tcttagacta
    10381 ctttaaccca ttcgtgatta ctgcagtatt tagagtttat gcagaagaat tgtcagaagc
    10441 aagagaacat ccattaacta agagacgtta tatgtggaaa ctaacttatt aaaaaataga
    10501 tcaaactaaa taaggtggct tgagaaatta agccaccttg ttttcttcaa tgtgatttta
    10561 tacatatggt aaaataacta gtacatatgg taaaataact agtacatatt gtgaaagcag
    10621 gtggcaagat gcagcgatta aatgactatc tttatcaact aacattgctt ttgaaatcta
    10681 cgccttcaga taattattca gctcgcagta tttcgcaagc tttacagttg aatagaagta
    10741 caatcagtag ctacttaaat gaaggtgttc gtgaagggct tttcataaaa gtaaaaagtt
    10801 acccggtttt atttttacat agaacagctc ttgaagaact acatatcaca ctgaataact
    10861 ccgaaataga atcaattgaa agcttgttaa caatttctca aaaacccgca ttagatcaag
    10921 taattggctc taaaggaagt ttgaaagaag caattgatca aattaaaacg gcagtcttat
    10981 atcctggaaa agggttgcct ttattattga tcggcgctag tggatcaggg aaaacctttc
    11041 tagcgaataa aatatatgaa tatgcagtag aagaaaaggt cattcacaca tcggcacctt
    11101 tcatagatta taattgtgca caatatgtga gcaatccaga gttactatcc tctgctttat
    11161 ttggctacac gaaaggagca tttaccggag caagccagga gcatactggt ttgctagaaa
    11221 aagccgatgg aggagtattg tttttagatg aagtgcatcg cttatctgaa gaagggcaag
    11281 agaaattatt tacatttatg gatacgggcg aattctctcc tatgggggat aatagtattc
    11341 ggaaaaaagc cgatgttcga ttagtctttg caacaacaga aaatatatat acgactttct
    11401 taccaacttt tcttaggaga ttgccagtta ttgttaatct acctagattt caacaacgtc
    11461 cgtcgtttga acgtctgtca ttgattgatg aattttttgt ttctgagagt aaaattttag
    11521 ctaaggaact atctgtttct gatgcattga ttcatttctt aatgaatagt gattttgaag
    11581 gtaacgtggg gaatattaaa aatattatca agtatgcttg cggaaacgca tatatccacc
    11641 agaagagtca gcaaacaatt caagttaaat tattggatat gcctttagag tacagttata
    11701 aattcaagga gcaactaaat aaacccagaa agaaaagagc agatcgaatc tactatccag
    11761 aagaaacacg acaagtgcaa ctagaaagaa acaatcagct ttttgttcaa ttttttgacc
    11821 aattacttac tggattctct gaagtgattg aaaataacaa ggatattaaa ctatttttag
    11881 aagaaatggt taccaaggta acacaagtaa tggatacgct tatttttcaa gaggattacg
    11941 aaaaagagca gtctttatac gccattctaa actatcatat tcgtcaaagt attgatttta
    12001 tgcgagaaag ctacgggttt gaacaagatg gtaaccgggt agtcgctttg gccaactatc
    12061 tatatgtcaa ggataatcaa gtgatttttg aaaatagtct tcaagaa
//
LOCUS       DAZUMB010000083        12004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000083 DAZUMB010000000
VERSION     DAZUMB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.085"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..1934)
                     /gene="uidA"
                     /locus_tag="ACG80L_001955"
     CDS             complement(126..1934)
                     /gene="uidA"
                     /locus_tag="ACG80L_001955"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS5024173.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(1962..3728)
                     /locus_tag="ACG80L_001956"
     CDS             complement(1962..3728)
                     /locus_tag="ACG80L_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS5024174.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(3741..4535)
                     /locus_tag="ACG80L_001957"
     CDS             complement(3741..4535)
                     /locus_tag="ACG80L_001957"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5024175.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(4546..5922)
                     /locus_tag="ACG80L_001958"
     CDS             complement(4546..5922)
                     /locus_tag="ACG80L_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5024176.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(6051..6698)
                     /locus_tag="ACG80L_001959"
     CDS             complement(6051..6698)
                     /locus_tag="ACG80L_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5024177.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(6782..8209)
                     /gene="uxaC"
                     /locus_tag="ACG80L_001960"
     CDS             complement(6782..8209)
                     /gene="uxaC"
                     /locus_tag="ACG80L_001960"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5024178.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(8283..9362)
                     /gene="uxuA"
                     /locus_tag="ACG80L_001961"
     CDS             complement(8283..9362)
                     /gene="uxuA"
                     /locus_tag="ACG80L_001961"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5024179.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9365..10996)
                     /locus_tag="ACG80L_001962"
     CDS             complement(9365..10996)
                     /locus_tag="ACG80L_001962"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5024180.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11313..11984
                     /locus_tag="ACG80L_001963"
     CDS             11313..11984
                     /locus_tag="ACG80L_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5024181.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT         3811 a         2394 c         1573 g         4226 t
ORIGIN      
        1 aaagcgcctt tttttcgaaa aaactagaaa aaaatatcct tctttaaaca gaaaatcttt
       61 ttataaaaaa aaggagttgg caattaagca gccactcctt ttttatattc cttataaaag
      121 gtaaattatt ttttataact gaaatttggt attgatgacc atcgtttctt tagatagtga
      181 gcaatcattt taggctttct atctctagta aagattcctt tcttatttcc ttgcactctt
      241 aaaatccctt gacttgtttc aaaatctgca aaattccaca cttgttcacc aacaaaattg
      301 tcacatttat ctattacttc gtgatttgct tcgtaataac ccacttgata ctcttctgta
      361 aacattgttg gtactgtatc atgaagtcca gctactgtat ctgctccata ttctgtaaac
      421 attattggcg tatcgggaca tctttccatc catccatcta gctctttttt caacagatat
      481 tttgcatgtt ttaggtctcc acctgaaaca taccaaccat aatagcgatt taaacataaa
      541 acatcgacaa tatctcctac tttacaaata tctggtgttg actttaagta agtaacgatt
      601 gttacaggtc tcttctgagg gtcaagttct ttagtcaatt caactaaagg ttcaaaatat
      661 tctttagcgc cttcagaatc cgaatctggt tcatttgcaa tagaccacat tacgacactc
      721 ggatggttct tgtcgcgttc tatcaattcc ttaatcgcca ttttgtgagc attttctgtc
      781 cgtatttctt tccaagtatc atgtttttct acgttgtcat ctaataaagt agccatgaaa
      841 ttcaaatgta atcctactgc aggtacttca tcaataacta caataccttc tcgatctgct
      901 aagcgcataa tttcttctga ataaggataa tgtgaagtcc taaatgaatt agcaccgatc
      961 catttcatta aattaaaatc tttgatattt actgcttcat tcaaaccacg accattaata
     1021 tacgtgtcct catgcttccc aaatccctta aagtaaaaag gtttattatt aatcaaaaac
     1081 ttcccattcg aaacctgaat actacgaatg ccaaattctt ctgtgtacgt atcaatcact
     1141 ttattttcgt taattaaact aactttcata ctatataagt aagcatttag aggctcccaa
     1201 agtgttgggt tcttaagttg aatcactcct tctttcccct tagcttctgc tataagttga
     1261 gcttcttcgt catagattgc tattagaatt tctccggtcc attctgtatc gatattaaat
     1321 tctacatttg catctgaatc aattattttt gtattaatag tgacatcctt tatatagtct
     1381 ttcggagttg tatacaattt aacgggtcta ttaatgcctg cataattgaa aaagtcaaaa
     1441 ttaaccaaat tttctttttt tatgtctcca tcttcctgta tcgtttcttt caaaatccct
     1501 acaggtaaag tgctatcgtc aataacattg tttacagcca ctgtaattct gttacttcca
     1561 tcttttataa actgattaat ttctgattca aatggcgtaa accctccttt gtgttgatta
     1621 atcaattttc cattcaaaaa tacttttgct tcatgtgttg cagatccaaa acgaagaact
     1681 aatctttctt ctaataattt tttaggaaga atcacttctt tttcatacca cacccatcca
     1741 acatgatttc taatgttctc tgtttcaaat aaatcattat aagaagaagg aactgaaatt
     1801 ggataactct ctttttcaac cattggttct aaagcaagtt cttcttgtaa gtcatcattt
     1861 gtatctaact taaatctcca aagaccgctt aaatcaatta cttgcctcgt ctctgtcata
     1921 atgggatata acatgtaaat cactcctata taatttaaat attaaataaa tgtatccttt
     1981 aacccacaat aagcatctat tggacttatt cccttaaatt cagaaaggcc cattagtttg
     2041 tccactaata tttctaaagt ttcttgtttt ccatcataag tattgatata agtttttact
     2101 tgaggcacat cagctaaatg aaacggacac tgtacagata caaaaattgt tggaatttca
     2161 tgaatataaa atggaatatc cggagtacct ttacttgctg gccaaacaat tctttgaact
     2221 gtacctgggt taacattagc tatattaata atcaagtcat atttttcagt taaatctttg
     2281 attggtctct tagcagcata tattccagct atatttttaa ttttttcttc ttgcgataat
     2341 cctttcaatt cttcttcagg tgaaatgtat ttttcaactt caaatccttt tgctgacaaa
     2401 atctgtttca ataaatcaat ggcctgaggt ctatttccag caactacttc accaaaccca
     2461 ccttttaccc catgtacatc taccagtaaa atccttttat atttttctgg tacaataggc
     2521 caaatatctt gtttatcttt aactagtgta attgcttgat cagctacctc tttaaaaatt
     2581 gatttatttt caattaaacc aattttttcc atagcttctt gtttcggtaa caaaatttta
     2641 ttcttttctt ttttatgcaa acctagttta gcctttaagc ctaaaattct tcttaatgca
     2701 tcacgtaaac gctcttctga aataattcct ttgtaataac cttccatcat ataattaaag
     2761 tcttcatctg gatcattaaa aaataggaat agatcacatc cagccgcaat agaagtgggc
     2821 aataattctg accgtttcat tgccccagtc attcctaaca tatgactagc atcagtaact
     2881 actaatccat taaacccgag ttttcctcta agtaagtcag tgattaattc ttttgataaa
     2941 gttgcaggaa gtaaatcttc atctttcaga tcaagattaa aatgttttga ataagctggc
     3001 atatggatat gacctgccat aattgaaggt aatccagctt ctattaagcc tgagtatact
     3061 tttccaaaag aattatccca ttcttctgta cttaaactat taatactaaa agatagatgt
     3121 tgatctcgtt cgtcaatacc gtcaccaggg aaatgtttag ctgcaggcaa aattccactc
     3181 tcttgaatac ctttcatata agctaaacta agttctaaaa ctttatcagg gctttgtcca
     3241 aaagatcgac tagaaatgat aggatttctc caatttctat taatatctac aataggagca
     3301 aaactccagt tacatccaat agccgatgct tcaattccag aaactctccc taattcataa
     3361 gcatatttcg catcatttgt tgcaccaatt tttacttcta caccaacttc ggtaccatct
     3421 gtacaagctc cgttaccacc tgcttctgta ttagcagcaa ttaataaagg aattttgctt
     3481 tctgtttgta ggatatagtt ttgatcatat acctcatcag cagttcctgg attatatcgt
     3541 acagctccaa tatggtactg attgaccata ctacttaagt aatttttatc acgactagat
     3601 cccatattaa caaataattg acctattttt tcttctaaag tcatagcatc aatcgtctct
     3661 tcaatccaag aaatagcatc ttcatctaaa tgatatggtt tttttcgtaa gtctactttt
     3721 atagtcattt tctttctcct ctacttatta gtaagatttt tccaaaaagt atctatatat
     3781 ttttgttcaa tatctatata ctctcccgat aaacatttaa ataaaatatc agttcttaag
     3841 ttttcccatg attctttttc tttattaacc aaaataggat aaaagaaaac tttattctta
     3901 tttgcagctt ctagatcacc aggagaatca ccaatcatta atatcttatc caaatcacaa
     3961 ccattttcaa ttacacttcg aataatgtct tctttttttc ctttatcttg acaacacagt
     4021 tcatcaacta aggataataa ttcatgtctt tcccattctt cttgaatagc ttccttatta
     4081 gctgagctta caataattat cttcccatat tttttcagag cttttatagt atctctagca
     4141 cttaaaaagg gtttatctaa tcctttagct tgactaatct gtcgattgac ttcttccgac
     4201 cattttaaag cttttaataa atcctctgac tgagagtatt ctatttccct cttcaaagat
     4261 ggattagaca attcttttgt tacagatacc cagtgcttta atcgattaat cgatggtaca
     4321 tcattttttt ctaatgtttt aactaaacct aaaaatctgt ttattccacg agtctctgaa
     4381 tataaattaa tattattcca atcctgtagg aatttttccc tatgatgaac atcaaaaatc
     4441 tctgcagcaa ttggcccgaa aaagagattg tgtttataag tcattgtatc catgacacaa
     4501 ccatctgaat caatacaaaa tatccaactg ttcatgtttt ttctcctagt tatgtttttg
     4561 atttagcaaa ttataatttt ctaatacttt ttctttagat aatggatata cgaaaaataa
     4621 aatcaatcct acaacaaaga aacaaactgc tggaatcaac gttgaagagt tatacagctt
     4681 ttgtaaaact tctccacttt gtacttttgc taacgaatca taccctatta tagaaagagt
     4741 ccaacctccg acaccgccag ctaaagcttg tccaattttt cgggcaaaag aatatactgc
     4801 ataaatagtt ccatcttctc tttggccaga tttcacttct tgatcatcga ttacatcagt
     4861 aatattcgcc caaatgactg tattaaaaat acctaacccc atatatccaa cagaagtaag
     4921 aactaagaat acatacatat tactaggctt tatcaaaaat aaaagtaaat atacaatagc
     4981 agagaagaac atccctactg acgccaattc ttttttaccg tattttttac tgaaaattaa
     5041 acttacaggt gaaacaagta ccaacatcaa aataggattg ataaagttca ttaaaactat
     5101 tccctgtgca ttattataat aatctggaaa tacataattg ttcatactca ttgttaaaag
     5161 ctgagcaagc aacaaaaaaa tagctgcagc tattatagaa agcaacgctc tatttttagc
     5221 taatcctttt aaagatttca acaatgaaac ttttaactgc gattcatcac ttttaacccg
     5281 ttctacagtt aatttataac atattacgta acaaataact gcacacactg aaaatactcc
     5341 tgccacaatt gtaaaagttg atccaccacg tacaatttga gctccagaac tatctcttgt
     5401 ataaataaaa attggtgtta acacgccaat gaacatacct gctaaggtag ctcctatact
     5461 acgaaatgtt gataacgctg tcctttcttc tgcaacagaa gtgatagctg atgccattga
     5521 accgtaagga atattaatag atgtataaaa aatacttccc catagtatat atgttacata
     5581 catatatata actttaatac tcatagaagc tgatgccatt gatgtttgat acattaaaaa
     5641 acttgctaaa gcgactggac cactcatccg taaaatccac ggtttaaatt tgccagtttt
     5701 agatggtttt gatccatcca caatacgccc cattgttatg tctgtaactg catcaataaa
     5761 tcttgctact aaaaataatg ttcctacggt agctccgctt atccccaata cttttgtata
     5821 aaaaatcatt aaaaaagaac tagcaaaaat gaaagtgaaa tcattcccaa aatctccaaa
     5881 catatatcct attttgtctc ttagaccaaa ttttctttcc atataatttg cgctccttcc
     5941 tattgttaag cgttttcatt ctgttgaact gccaacttac gtactatact ctgaagtata
     6001 aagagttagt atttattttt tacattttat agtattattt attttttctc ctaataattc
     6061 tttatctcat aaaacttatc tatatattta ctagctattc gagttacttc ttcaaaatca
     6121 cctttttcaa caggagccaa caaattccca cctacaccaa ctgcaacaac gcctgcttca
     6181 aaccactctt ctaagttatc tagacttact ccgccagtag gcattatatt tacatatggt
     6241 aatggagctt gatacgtttt tacaacagat ggaccaaaag cagaacctgg aaatagttta
     6301 atgatttcta ctccagcggt aagtgcctcc tttatctcag taattgtcat acaacctggt
     6361 aaataaggaa tttgatataa attacaaatc tctgctgttt gtcgatcaaa agatggactt
     6421 acgatatatt ctgctccagc cataattgca atacgagcag taaacgcatc taaaactgta
     6481 ccggctccaa ttattacatc tttttgatca gcatattttt tagcaatttc tttgattatt
     6541 tcttcagcat tcggaactgt aaaagttagt tcaatccctt taattccacc cgcagtaatt
     6601 gcatttattg ctttaatggc ctcatcttta ttttttccac gtacaaccgc aatgactcca
     6661 gccgcttcta acttacttaa aatttctact tttttcatat ctatctcctt cttttatgga
     6721 tacatactct agctagttgt actaacttac aataaataaa tttaagcttt tattttttgt
     6781 attattttct actttcgatt tcaaaaaaat taaaataatt atacgcatta cgataacaaa
     6841 tattctccac tatctgacta agatacattt catcatctgg aattcgacct gctttaaccc
     6901 actcgcttag taattgacat aatactcgac ggaaatactc atggcgagga tagcttagaa
     6961 aacttcgaga atcagtcaac atacctgtaa aattcgacaa caaactttgt tgcgccatcg
     7021 tagtcaactg ttgacgcatt ccttcagctg tatcattgaa ccaccaagca catcccaatt
     7081 ggattttttg aatcataccc ccttggaagc tttgcattgc tgttgctagt ggaagccaat
     7141 cggttggatt taatgaatat aaaattgttt ttggtaagca cccactcttt tcagcagtgt
     7201 cgaacaatgc tacaacattt tgtgtcaaac tagcttgatc ccccatcgaa tcaccaccga
     7261 catctactcc aatttgttcc tttaatatac ttgaattatt tcttaaagca tttaaatgca
     7321 tttgcatcgt ccaaccatat cttgtatact cagacatcaa atgtaattga atacctgtaa
     7381 caaattgtgc aatttctgat aaatggaaat ccgtttttcc tgctaaagct tttattaaca
     7441 gattgtcgag tattttttca tctgctattt cataaaaata agtattcaaa ccgtgatctg
     7501 ctaatcgaca gccaatttga tgaaaataat ccactcgttg gctcaatgcc tgcttcaacc
     7561 cattaaagtt tttgattggt atacccgctg cttgactaag ttttaaaata tagttcgaaa
     7621 atcctgatga tttgatactc cagaccttat ctggacggaa agtaggtaat accttgaaag
     7681 aattatcctg tttagaaagc tcagtatgat atcgcaagtc atctgtagga tcatccgttg
     7741 tacaaatggc tttcacatcc atcatcttca gcaattcttt tgggcgatag gaatccgttt
     7801 ggattttttc gttggcacgt cgccaaattt ctggtgcatt cttctcagaa attgtcaaat
     7861 caatatcaaa gtaacgttgt aattccaaat gagaccattc aaaaatagga ttacctggcg
     7921 ccttctccac tgtttttaca aaagctaaaa atttttcata atcattacct gcaccactga
     7981 tcaactcttc ttctatccca ttagctcgca tcaatcgcca tttataatga tctccagctc
     8041 ctttatcatt taaccatacc tgagtcaaat tcttgtattg cttattttct agcaaacatg
     8101 ctgggtctaa atgacaatga taatcgatga ttggttggtg ttctgcaaat ttatgaaaaa
     8161 gtcttttagc tatttcattc tctaataaaa agttcttatc taaaaacata tttaagcact
     8221 cctttccttc ccttagtttt tagaagagaa aatttttctt ctaaatagac tccattatat
     8281 aattatttac tcgccccctt ttcaagcgct tcccaaatac catttaaata aactgcacct
     8341 agagctcggt catataaccc ataaccaggt ctaccggttt ctccccaaat catccgacca
     8401 tgatctggac gaatatagcc attaaatcca ttatcgtaca ttgtttttaa tatcttatac
     8461 atatctagag aaccatattc tgatttatgc ggcgcttcat aaaaatcgag tccattatca
     8521 cttaaatatt tgatattacg aacatgtgca aaaggaatcc gatcccgttg acaaaattca
     8581 gatagaattt catagacatc attatctggt cgttctgcaa tacttccagt acataacgta
     8641 ataccattag aaggagaatc cactacatta cacaaccatt ctaaatcgtc acgattttta
     8701 acaatacgag gtaatccgaa aatagaatat ggtggatcat ctggatgaac tgccatttta
     8761 atcccaactt cttcacatgt cgggataatt gctttgagaa aatatgcaaa gttctctcgc
     8821 aatttcgctt catcaacttc tttataggct tctaataatt gcttgatttg tgataaccga
     8881 tgaggttccc aacccggcaa ttcaaaatta ttagtagaat tttctacttt tctaataatt
     8941 tcctctggat cagtaggaat attttttgaa atataggcta acgtagaaga cccatcatct
     9001 aaatgataat ccaaatctga ttttacccaa tcaaagactg gcataaaatt ataacagata
     9061 acctcaatcc caaattctgc caaattgcga atcgtctgtt tataattctc gatgtattga
     9121 tctcgtgttg gcaaaccaat tttgatatca tcatgaacat taacactctc aataactttc
     9181 aaggtaaggc cattggcttc gacttcatct tttaaagctt taattcgttc tctcggccag
     9241 acctctccgg ctggtatatc cattaatgtt ccaacaattc ctttcattgc tgggatctga
     9301 cgaatttctt gtagtgagat tttttcttca tttgccccat accatctaaa agtcatttcc
     9361 atagttactt tcctccaact tctgttatat atttattgat tgttttacga actgcttgag
     9421 gacctactag catttcgcaa aagatttttt caattttctt tcctatctca acttgatata
     9481 aatctaaacc aaatatttcc ttattttcta agatttcctg taccgcacaa tgaatctttg
     9541 caggatcttg ttcacctaat ttcaatactg agaatttttc ctgcaactct accaataaag
     9601 gatcaggact tggcacaaaa ttttttcctt gatcatcgac tgccaataaa taacgtaacc
     9661 aagcagcaat agcgatagga ataaatgtta gattttctgc atttccgtct ttttcgacat
     9721 actttttaat tgtttcacca tagcgaatcg ctactttttg agaagtatct gttgcaattc
     9781 gttgcggtgt atctggaata tagggattag gcaaacgttt attgattact tcatcaataa
     9841 attgttttgg atcaataacg cctggacttt caactactgg taagccttct acataaccta
     9901 tccctttaat taagccaacc aaatcatcat cttgcatttc tgcctgaatt gatttatacc
     9961 ccaacaaaca acctgttatt gctaatgccg tatggagcgg atttaaacat gtcgtaacct
    10021 tcatgatatc tgctttatca actgtttctc tatctgtcat aatcacacca gccgcttcca
    10081 gtttaggtcg accatttgga aaactatctt caatgactag ataatggact ttttcagtat
    10141 tggtaaaagc agcgatattg gtatttttcg cagtatgaat tatatctaag tcttcgaaac
    10201 ccgccttcaa taaatccatt ttgacacttt ccgaaggatt tggcgtaatt ctatcaatca
    10261 tactccaagg aaaagtcact ttttctttat cctttaaata agcaataaat ttttcagaaa
    10321 cgtatccttt tttttgccaa gcttctgcaa tcataaaaat gctatcttga aaacgttgac
    10381 cattctgtga gaaattatca gtagaaacca tcgcaatcgg taactctcca gcattaaacc
    10441 tgctatacaa caaggatgtc acaatactca tcgtatgttg aggattggta ggtccagatt
    10501 ttatatctgc ttccactacc gaaaaaaatt gtcctttaga atcttttaaa ctgtatcctt
    10561 tttcagtaat agtaaacgtt gtaaactgta atgtaggatc ttcaaaatat ttaaccactc
    10621 tttgatagtc cgttatattc tttggattgc aatataaact atcagctgtt actgctaaaa
    10681 gtcttttttt gagttcacca tcttcaccca taacaatctc caaaatatca ttgttgtagt
    10741 catgataagc tttttctact acttcttcat caaatgtctc acacacaact actccggatt
    10801 ctaattcatt ttgatctgct aagcattgag caatttctgc atgaaatcct cgataaagat
    10861 ttcctccacc aaaatgaagc cacactggat gtttctgacc ttcttgtttg accgcttcga
    10921 cattaaaata cggtagttga acttgcattt tgttcaatcc ttcgatagaa tcttgccagt
    10981 ctttaatcaa aaccatattt acccctccat actgatatgt tagtttccgt tacaattgta
    11041 gtataaaatc gaatgtaatc gctgtcaata agaaaaaact aaaaatattc ttttttctac
    11101 catgtcagaa ttattgtgtc tttttttagt aaaagcacac caatatatat tttttagaac
    11161 taacttaaat taaagcttaa tttctctttg atcttatttg agtatttttt gtttttttaa
    11221 tagctagtat gttactttga aatttgatat tatataacga gcatagaact aaaatatcaa
    11281 attcaaaaat gaaattttga gagggattaa atatgacaat taaaactagt ctccaaaatc
    11341 aagcttatca atctattcgt cagcaaatta tctacgctga cttagaacct ggaaaaaaag
    11401 tatcagaaaa aaaattggaa gaaacgcttt ctatcggtcg tactccaata cgtgaagcat
    11461 tgattcaact ccgtcatcaa ggacttgttg atacaattcc tcaaagtgga aattatatct
    11521 cattaatcga cttagattta gcaaaaaatg ctcgttttgt cagagaacat ttagaacgac
    11581 aaattatgct agagtgttgt gccaagatga ataatcaaaa gaaacaagtt ttagaaacta
    11641 taattgcaga acaagaaaaa gctgttagtc aaaagaatgc tcgtagtttt tttcataatg
    11701 ataatttatt tcataaagct tgttttgaaa ttgcagaacg ggaagaagta tggcaatggt
    11761 tggaagataa taatacgcac ttggaacgat ttcgttggtt acgagtaaaa acggaaggat
    11821 taaaatggga aaaaattatg gaacaacatc atcaactatt taaagccttg accatgcaag
    11881 accctgaaga agctaacttc ttaacatcct tgcatcttca tttaatgctt gatgaacaaa
    11941 atgttgtatt gactactcac ccaaaatatt ttaaaaatat ataactgcta aatttctttt
    12001 tttt
//
LOCUS       DAZUMB010000084        11698 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000084 DAZUMB010000000
VERSION     DAZUMB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.086"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(186..1355)
                     /locus_tag="ACG80L_001964"
     CDS             complement(186..1355)
                     /locus_tag="ACG80L_001964"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5024182.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1559..2734
                     /gene="mltG"
                     /locus_tag="ACG80L_001965"
     CDS             1559..2734
                     /gene="mltG"
                     /locus_tag="ACG80L_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS5024183.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2870..3349
                     /gene="greA"
                     /locus_tag="ACG80L_001966"
     CDS             2870..3349
                     /gene="greA"
                     /locus_tag="ACG80L_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS5024184.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3518..4249
                     /gene="liaF"
                     /locus_tag="ACG80L_001967"
     CDS             3518..4249
                     /gene="liaF"
                     /locus_tag="ACG80L_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS5024185.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4246..5313
                     /gene="liaS"
                     /locus_tag="ACG80L_001968"
     CDS             4246..5313
                     /gene="liaS"
                     /locus_tag="ACG80L_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS5024186.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5320..5952
                     /locus_tag="ACG80L_001969"
     CDS             5320..5952
                     /locus_tag="ACG80L_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5024187.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6092..6757
                     /locus_tag="ACG80L_001970"
     CDS             6092..6757
                     /locus_tag="ACG80L_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS5024188.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6785..7078
                     /locus_tag="ACG80L_001971"
     CDS             6785..7078
                     /locus_tag="ACG80L_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS5024189.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7146..8093)
                     /locus_tag="ACG80L_001972"
     CDS             complement(7146..8093)
                     /locus_tag="ACG80L_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS5024190.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8271..9074)
                     /locus_tag="ACG80L_001973"
     CDS             complement(8271..9074)
                     /locus_tag="ACG80L_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS5024191.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9220..10134
                     /locus_tag="ACG80L_001974"
     CDS             9220..10134
                     /locus_tag="ACG80L_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5024192.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10182..10652
                     /locus_tag="ACG80L_001975"
     CDS             10182..10652
                     /locus_tag="ACG80L_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS5024193.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10737..11345
                     /locus_tag="ACG80L_001976"
     CDS             10737..11345
                     /locus_tag="ACG80L_001976"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS5024194.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11479..11679
                     /locus_tag="ACG80L_001977"
     CDS             11479..11679
                     /locus_tag="ACG80L_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5024195.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         3930 a         1809 c         2362 g         3597 t
ORIGIN      
        1 aatcttagaa taatttggac agaacagaaa gaagaacacc tctttctgtt ctgtttattt
       61 ggaattaaag tctgtagcaa aaattgattt ttagatttgt cccacactct ttcctacgtt
      121 tagtcgagta ctttttttaa gcaaatgcta ttcaaagaag tagtatttgc tatcttttca
      181 tcatatcagc tatttgccaa cttagtagtt ccttccatca ggtcatagat ttggtttctt
      241 agatcagcag taggcagttt gatagttttg tcagctttta tattgctttc catgatgata
      301 gcatcctgtg aatctttgct aaatatagca gatgtatcaa aagagtcgat atttccttgc
      361 gacaggatat aattatctcc gctatatacc ccaccactat atggatgtcc attcgcattt
      421 tcacgccata atgagaagag taagtctttg gaaactagtt ttccagataa aacttgtgta
      481 aacagcgtat ttaaatcata gacggacata ctgatattac cagtccctaa ttcgttatgg
      541 atctctgttt cagattctgt cagtggttta aagtaatcat cctctttaga catttgatag
      601 gatagtgcat gattggatga atttttgact tggtcataaa attttgtatg ctgtaatcct
      661 aaaggttcga tgatatcatc ggtcaataat ttttcatacg gttgatttgt taatttgatc
      721 aaaattccag tcaatatggt aaagttcaat gaatcataat gagaggtctg ataatctgta
      781 aattttaagt gctgaatcgc aaattgaacg acttcttcat cagtcatcgg tatggttgga
      841 gaagcagttc ggtgcaagcc tgagcgcata tacagcaagt cacggatagt aatttgctgg
      901 ctgccagcaa tttgtggata gaattgagcc agcttagtat ctaaagaaag gtgcccacgc
      961 tcgacttgtt ttaaaatcaa aagagtagtc aatgcttttt gaaccgaacc aatctgataa
     1021 taaagagaag gatcatttgg ttgttgcgtg agtttattcg catagccata gcctttttcc
     1081 atgatgatgc gattattttt ctttacgtaa attgagccaa tataatcatt ttttcttaat
     1141 agttgatcga ttttttcaac gagctgttgt tcattggtag tcagtatcaa ctttttttga
     1201 gctgattgag catgggcaac tatctgagag tgttcattat tgtaaaattg ccatccaaga
     1261 atagttcctg tagagaacgc actaaggcta aacaacacga caacgatcat taaaaatgaa
     1321 tattttcttt ttttagcatg ttttcctttt ttcataatag tttatttagt ctcctttctt
     1381 ttttacagaa agaataaagc taaaatagct tgtatcttga taacatttat tttaatctta
     1441 tagtaatgga tttactactt acatttttga tagaatattt aaataaaatc attcacgcca
     1501 attcaaatat catgtataat gaataaagtt acgaacttga tagtaaggag gagttgtttt
     1561 gtcaggaaac aaacaacacc caagtaaaga taaaaaagaa gccagtaagg cagctacagc
     1621 agatataatc gacaagcgga gaaatctgcg taaaaaagaa gataagattg tgaggaagat
     1681 cattttagtg attgctctta cattattgat tattggcgga tttcttggat ttaccgtgta
     1741 tcgttatgta gatagcgggt tgaagccttt agataaatca gatgaccaat tagtccaagt
     1801 cgagatcccg agtggctctt ctaacaagca gattggtgag attttggaga aggacaatat
     1861 tattaaaagc gggattgttt tcaattatta tacaaaattt aaaaatttaa ctggctttca
     1921 agctggctat tatcagttag cgcctaatat gactcttgac gagattggca aacagttgca
     1981 agaaggcggt acttctgagc caacaaaagt tgcagatggg aaaattgcca ttccagaagg
     2041 gtacgatatc gatcagattg ctgaacgggt agcgaaggta actggtaaag acaagaaaga
     2101 atttttagat ttagtgaatg atgagacttt ctttaacgag ttgcaccaaa aattcccaga
     2161 actgttggaa agtgcatcta aagctgagaa cacaaaatac cgtttggaag gttacttatt
     2221 cccagccaca tatgattatt acacgagtac ttcgcttaag gatctagtga ttgaaatggt
     2281 gaacaagacg aatacggtca tgcaaaacta ttattcagct atcaagcaga aaaatttgac
     2341 tgtacaagaa gttttaactc tagcttctct agttgaaaaa gaaggcgtaa aagaaaatga
     2401 tcgtaaaaac attgcacaag tcttctttaa ccgaatcaaa gcaaatatgc cacttcagtc
     2461 agatatctct gttttatacg cattaggtga acacaaagaa ttagtgacgt atgaagatac
     2521 agctgtagat tcaccatata atttgtatac caataccggc tatggacctg gaccttttga
     2581 caatccaagc gaagaggcaa tcaaggcagt attagaacca gcagaaaatg actattatta
     2641 tttcgtagca gatacgagca ctggaaatgt ttatttcgct aaaacttatg aagaacatat
     2701 ggaattagtg gaaaaatatg taaataattc ttagaaaaac aggctctaga cattgatcat
     2761 ttttagaaaa acatttacat ttgaagaaaa gcgtggtaat cttttttgga ctatattcct
     2821 caaaagatta ccttttgcgg tattttatcg aaaaaaggag acgttacaca tggtagagaa
     2881 agtttatcca atgactctag aaggtaaaga aaaattagaa aaagaattag aagaactaaa
     2941 aacagtcaaa cgcggagaaa tcattgaacg aatcaaaatt gcccgcggtt tcggagatct
     3001 ttccgaaaac tctgaatatg aatcagcgaa agatgaacaa gcatttgttg aaggaagaat
     3061 cacaacaatt gaaaatatga ttcgttttgc tcaaatcatc gataacgacg gtgtagatgc
     3121 agatgaggta tcaattggta aaacagttac attcatcgaa ctaccagatg gagaagaaga
     3181 agagtacacg attgtcggaa gcgctgaagc agatccgttt tctggcaaaa tttccaatga
     3241 ttctcctatt gcgcaagcat tgattggcaa acgtaaaaat gatgaagtaa ctattgctac
     3301 tccaggtggc gatatgttag ttaaaatcgt caaagtagaa caagcgtagt ctgactgaaa
     3361 gatctagaga gttcatctta ctgatgatac actctagatc tttttctctt tgtgtatttt
     3421 agatttcaac aaacgatctt acatctaaag tcctatagcc aacagttaaa aaaataggta
     3481 tgatgaaatg agaatgtgtc aggagggaga aagagcgatg aatagttctt ggcgtttttt
     3541 tgtggtagtt gaagcactac tgctgttatt tgctgtttgg caaatcgtaa acaatactgg
     3601 acttttgctt ttagtgatat tcggtatttt caatatatat ctagctatgc gcaaacatcc
     3661 tcgtactaaa tttcaaaatt ttcaattgat attaggaagt ttggtcattt tttttagctt
     3721 agtcaacagt cctgctttat ggatgatggc tgtatttgcc gttttattta ttggtctgaa
     3781 aggtgtagag atttcaggga tagatttaac caaaaatgct ttttggcgaa agaaacagat
     3841 catgatgatc caaacagagc aattaaaaac acataataat gagcggaaaa gacagcaatt
     3901 attcggtaac caacgtattg gaaacgacgt ctatgaatgg gacgatatca acatcgccgt
     3961 catttctggt gatactatca tagatttggg caatacgctt ttgccaaaag atgataatat
     4021 tgtgattgta agaaaaggga taggcagaac acggatctta gtacctttgg gcgtagccat
     4081 ccgattagag catgcgacat tagtaggaaa tgtcttgttt gaagaagagc aattttcttt
     4141 gaaaaatgaa caaatcaaaa tctacagcag tgattatgat gaaaaccctc gtagattgaa
     4201 gattattact aataccttga ttggagatgt cgaggtgata cgaatatgat gggaaaaata
     4261 tccagagcga tgctagctgt ttattcgggg attgcggctt tccttattat tctattttca
     4321 cttttcactt atttttatgc cagcaatcaa agtcattggt ggggagaatt actgcgtgca
     4381 cgtttattat atgttccgct tatcttccat ttgttagcca tttccttagg tgtaggatta
     4441 attgtctttc tattattatc acttattcaa aagacaaaat acgggaaaat cgaagaaaag
     4501 ctgcgtgcac tttcttctgg caattatgaa tccaaattgt tgcttcttcc gatcccaagt
     4561 gcatcggacg atttatacat caaagatatt gataaggaaa tcactaagat aaaagaaaaa
     4621 atgattgaag tatctagtga attacaaatt gtaacgagcc gtccccaata tgtagatgga
     4681 caaaccaaag aagaaatttt agaattagaa agacatcgat tagcccgtga gctgcatgat
     4741 tcagtttcgc aacaattgtt tgcagcaatg atgatgatgt cggcattgac ggagcaagca
     4801 gaaaaaagcg agacaccaga aatgtttcgc aagcagttga aaatggtagc agagatcatc
     4861 aacgcttccc agtctgagat gcgtgcgctg cttcttcatc tacgtccagt caatttagaa
     4921 gagaaaagtt taaagcaagg tatcgagcag ttattgaagg aattgcaaaa taaaatccag
     4981 atttcgctga aatgggatgt agaagatgta aaactatcta gttccattga ggatcacttg
     5041 ttccgaatcg ttcaagaatt gttatcaaat acattaagac atgctaaagc caatgaatta
     5101 gaggtatatt tgcacaaaat agacaataat cttttgttac gtattattga cgacggaaca
     5161 gggtttaata tgaatgaaac gaaaacggga agttatggat tgaacaatat caaagaacga
     5221 gtagctggta ttggtggaac agtaaaaatc attagtttta aaggtcaagg tacaagtgta
     5281 gaaatcaagg tccctttgat gaaggaggca tagataagga tgataaaagt tttattagta
     5341 gatgaccatg aaatggtgcg cttaggcgtc tcttcttatt tatctattca agaagacatt
     5401 gaagtaattg gagaagctga aaatggacga caaggctatg agaaggcgat gacacttcga
     5461 ccggacgtca ttttaatgga cttagtcatg gaagaaatgg atggtatcga atcaacaaaa
     5521 gcaatcttaa aagattggcc agaagctaaa atcattatcg taaccagttt tattgatgac
     5581 gaaaaagttt atcctgccat tgaagcaggt gcagcagggt atttgttgaa gacctctacc
     5641 gcacatgaaa ttgctgatgc tataagggca acgcaacgtg gcgagcgagt attagaacca
     5701 gaagtgacaa caaaaatgat ggaaaaaatg agtcggcgaa atgagcctgt attacatgaa
     5761 gaactaacga atcgggaaaa tgaaatttta atgctgattt ctgaaggaaa aagtaatcag
     5821 gaaatagcgg acgaattgtt tattacactg aaaacagtaa aaacacatgt ttccaatata
     5881 ttggctaagc tagaagtgga agaccgtaca caagccgcta tctatgcatt caaacatggt
     5941 ttggtgaaat aaaaacgatg ataaagagga taatcaaaag ctgagaaaat aactattatt
     6001 tttcttggct tttttacaat aatgatgctt gcttttgttt ttgtaaaggc tttgttatac
     6061 tgagaaaaga agatactaaa ggaggaaaac ggtgaaacaa aattttgcaa ttgtaggtct
     6121 tggacgattt ggcggaagta tctgccgaac actgatcgaa gctggtcaag aagtactggc
     6181 tattgatagt aatgaagatc gggtcaatga atatatgaat atagccactc atgctgttgt
     6241 ggcaaatgca caggatgaaa tgacacttcg atcattaggc ataagaaatt tcgatcatgt
     6301 aattgtcgct attggagaag atattcaagc cagtatctta gttactctga tggtaaagga
     6361 aatgggcgta ccaaatattt tagcaaaagc ccaaaatgag tatcatgcca gagtacttga
     6421 aaagatcgga gctgatcgcg tcgtccatcc cgagcgagac atgggaataa ggattgcgca
     6481 taatttagtg tcaaagaata ttttagatta cctagagttg tcagataaat attctttagc
     6541 agaaatccga gtctccaatc ctaaattttt caacaaaaca ttagctgaat taaatttcag
     6601 gcaacgtttt gacttaacag tagtcgccat acgaagagta gatcaaacgg ttatcgcttc
     6661 tcctgcagca gatgaatatg ttcgtgaaaa tgataatcta ctggttattg gagagacagg
     6721 cgatgtggat attctagacg ataagatgaa tcaatagaaa gaaaagagaa agtaggttgt
     6781 tgggatgaaa ttaacggtaa cgccaaaagc acaaaagtgg tttgactcag aaattgatct
     6841 gccagaaact tacggaatac gtttttttgg aaaagtctac ggcaaaacag aggttcatga
     6901 tggtttttcg attggaatgt cagttgaaca gccagaacat cctgtaaaga aagtggaaat
     6961 ggatgggaca ctttttttta tcgaggaagc agatgaatgg ttctttaaag gatatgattt
     7021 acttgttgat tatgatccca acctggagga gccaacctat cactttaaaa aacaataaaa
     7081 aaagagaggg tgacacaatt tttgtcacct tctctttttt cttttgaaaa tattgttttc
     7141 agctattaat atcctgcaga caattttact tgattggttt gaagctcatg ggtggcaaag
     7201 tagcttttca gattggttaa aaaaatagcc attaatttct tttgaaaatc aggtgtcaaa
     7261 cctgcaatgt gggaagtaat cagtacattg tccattttcc aaagaggatg atcttgtgga
     7321 agaggctctt cctcaaaaac gtctaatgct gcaaaggaaa gttgctggtt atttaaagcc
     7381 tgcaccagat cttgtgtctt aacagaagca ccacggccaa cattgatgaa tacagatttt
     7441 ggatcaaagt gttcaaacat atgctgatca aagagcccag ttgtttcctc cgttagaggt
     7501 aagatattga ctacgaaatc tacttcagga agaacttcct ttagttgatt taaagaaaaa
     7561 gtcttttcca ttccttctgc tggatgaccg gatgtattga ttccatagca gttcatgtct
     7621 aatgctttgc atttttttgc aatcattttt ccaatatggc cagttccaat gattagtatt
     7681 tttttccctt ggatagattg gtaatggacc gatgaaccta accatttcat gtcactctgt
     7741 gccttttgtg cttgaaaaag accacgagaa tacccaagga tgattcctaa gacatgctcc
     7801 gtaatcgatt cactgtggat gccactacca tttgaaagta aaatatgttt ctttgaaaaa
     7861 gtctcaaggg gtagctgatc aacaccagct gagatagatt ggacccattt caaggtagaa
     7921 gaggctaata gtttttcttg gtagtctgag ttccagccca aactgatctc tacattttgt
     7981 agatcctctg gttgtaattc tgttttaaag atataaccag gcgctatagt atggatttct
     8041 tcgataaatt catcacgaaa tggacgatct gcataaatga ttctttctga catagttaat
     8101 ttcccgcctt tctttactct aagtgtagcg catacaagaa gaactttgaa gcgccttgct
     8161 tgttttttga gaaagaaata tttttcaaat aaaaaagggg ctgtgacaaa aaaatgtcac
     8221 aacctctaaa agggtctatt gaagtagaaa taacgagttg tctttatttc ttaattgatc
     8281 gatatttttg cccatccttt gacagcttga gcaaaaataa agattcctgc aaaagcaatg
     8341 aaggcgctag tgtcaaaaga aggaagaaag accatcaaga ttgttgcaat tatagtaggc
     8401 aatgaaattg aatagatcaa tgtttttaaa caatcaagaa aagtagcttt tgttagtcga
     8461 aagcgtgcat agagaaaagc agcaatgttg gtaaatagca aagtgatcag cagattcaga
     8521 aaactaggat aaatagaaaa tataaatgtc aggactttta tccaaagtgg tatagatgct
     8581 tcacttaaaa aagaacggag ctgcttacct gttaagtttt ttaaagattc attggtgtag
     8641 ggaagttcta attggttatt gttcaacaaa gcagaagtcg tgcctgtatt aggaagagct
     8701 atgataagtt tgttttttaa catcccgaca ctaacaaaat tccccatcaa atcagtagag
     8761 atgtcctgtt ctgttcgttt tccctctgga tcaaatgtga agataatgga gtttgtttgg
     8821 taaatgaatc cttctttttc ttcggtttga agctgcccgt tttggattgt aaaatcagga
     8881 atgcgagtag caatcttttg cccatccaat tgaatgttgt ttaagacttg aaaaacttga
     8941 aaagagattg gcaaagccat aattatactc aaaactaaaa gatagacaat ggctttccaa
     9001 aatgggacat ttctcgcctc ctttaggtct ttgaatcgta taaaggaact gataattaat
     9061 tgttttgttg tcataagtac tcctcatcta gtagtgtcat cacttgcaat tctagcgaag
     9121 tatcaagtga aatacaagtg ctttaaggaa acagctgaat gattggtgaa atatcaaaaa
     9181 aatgttattc ttgtctaaag actatccaat ataaataaaa tggaggaagg aatagtgcct
     9241 acacagccac agagatcatc aaaaaaacta tttggagtag tggttttgaa tgcggtgatc
     9301 actttatcag aatttattgg agggattatg tcagggagtt tgtctcttgt ttcagatgct
     9361 ttccataacc ttagtgatac attggcaatt attttttctt acggggcaca aaaaatggcc
     9421 cgaaaagaag caaataaaaa acgtacgtat ggctaccagc gtttagaaat attgagtgct
     9481 ttcatcaact cattcatatt gatcatatta tccttgtttt taaccgttga agcattcaag
     9541 cggttcactt caccagaaaa gataaatagt catttgatgc taacagtagc agttattgga
     9601 ttgctcgcga atttgttctc agtattatta ttacgacaag aagcagatga aagtttgaat
     9661 ataaaatcta gttatttgca tttgttaagt gatacactgt cttcggtaag tgtgattatc
     9721 ggagcaattt tgatccgttt tttcgatatc tactggatcg atccagttat tacactgatc
     9781 atttcgatat atatattgat agaagcgata atggtgatca aaaaagctgc cgctattttg
     9841 atacaaagtg cacctgctat cgactatgaa aaaatggaac aagaaattga agcaatcgat
     9901 ggggtaaagg atgttcatca tgtccatatt tggcagtata gcgaaaagat aattattttc
     9961 gacggacata ttgattttga agatcagcta ctaagcgaga ttgaaaagat atatcctcgt
    10021 ataatttctt tattaaaatt aaaatatggt attacacatg tgacgatcca agcagagaca
    10081 aaggcaaagg atcaaaaaaa attaattttt ttaaataaag atgaaagtta ttgaaatata
    10141 gagtatgata aataagaatt tttaataagg taggttggct catgaagcag tatcgacagt
    10201 taaaaggata tttagaagca aaaggatatt gggagatact agtctatctc ttttttggtg
    10261 gtttggctac gattgtaaat tttgttagtt ttgctctcgc acgccaatat tttgatttaa
    10321 gtatggcctt atccaattct atttcgtggt tttgttctgt attgtttgct tttgtaacga
    10381 acaaactttg ggtattccat tcaaaatcac cgaattttac acatgcactt attgaatgcg
    10441 gtaagttctt cttctaccgt atcctttctt acggattgga catgggggca atggtattgt
    10501 tgatcaatgt tatgaacagc aatgagtatg tggcgaaaat catcactcaa attattgtta
    10561 tccttgcgaa ctatattttc agcaagttgt ttatttttaa agaaactgag gtttttgaag
    10621 aagaagtagg tcaaagtgaa aaagacgact aggcataaca attgaagaat ttccttctct
    10681 ttgttactct ttgtaatgta gacattatta agaaaacatt gaggaggaat ttttttatga
    10741 cttacacatt accagatcta ccttatgcat atgacgcatt ggaaccttat attgacgaag
    10801 aaacgatgca tctgcatcat gataagcatc acaatactta tgtgacgaat ttaaattcag
    10861 caattgagaa atacccagaa ttaggcgaaa aaacaataga agaattatta tctgatatgg
    10921 acgctattcc aacagatatc aagacagctg tacgtaacaa tggtggcgga catgctaacc
    10981 attcattttt ctgggaaatc atggcaccaa atgctggtgg cgaacctaca ggagaaataa
    11041 aagaagcgat taatgaagct tttggtgatt tttcttcttt taaagaagaa ttcaaaaaag
    11101 cagccgctgg acgatttggt tctggatggg cttggcttgt aatggaaaat ggaaaattag
    11161 ctattacctc tactgcaaat caagattctc cattgatgga aggaaagaca ccaattctag
    11221 gtttggacgt ttgggaacat gcttattatt tgaaatataa aaatgtccga ccagattata
    11281 ttgcagcatt ctggaatgtt attaattggg atgaagtaaa caaacgtttc gaagcagcaa
    11341 aataaccatt tcctccgtca tatcagtctt aaaaataatt tggaaaataa aagagcgaaa
    11401 gcttgctagc gttttctaaa ttgggtataa tgttcttgca aggttacttg caagaacttt
    11461 tttggaggat atacatttat ggaacacgga acggttaaat ggttcaacaa tgaaaaagga
    11521 tttgggttta ttacagtaga cggcggagat gacgtatttg ttcatttctc agcaattcaa
    11581 ggagacggct tcaagagcct agaagaaggt caagaagtag aattcacgat tgttgaaggt
    11641 gcacgcggac ctcaagctgc tgaagtgact aaattataat aatttgttga ataaagac
//
LOCUS       DAZUMB010000085        11520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000085 DAZUMB010000000
VERSION     DAZUMB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.087"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..669)
                     /locus_tag="ACG80L_001978"
     CDS             complement(124..669)
                     /locus_tag="ACG80L_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5024196.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(681..1373)
                     /locus_tag="ACG80L_001979"
     CDS             complement(681..1373)
                     /locus_tag="ACG80L_001979"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS5024197.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1392..1676)
                     /gene="macP"
                     /locus_tag="ACG80L_001980"
     CDS             complement(1392..1676)
                     /gene="macP"
                     /locus_tag="ACG80L_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS5024198.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1689..2252)
                     /locus_tag="ACG80L_001981"
     CDS             complement(1689..2252)
                     /locus_tag="ACG80L_001981"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5024199.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2480..3142
                     /locus_tag="ACG80L_001982"
     CDS             2480..3142
                     /locus_tag="ACG80L_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS5024200.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3266..4462
                     /locus_tag="ACG80L_001983"
     CDS             3266..4462
                     /locus_tag="ACG80L_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS5024201.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4563..5939)
                     /locus_tag="ACG80L_001984"
     CDS             complement(4563..5939)
                     /locus_tag="ACG80L_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5024202.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5976..6842)
                     /locus_tag="ACG80L_001985"
     CDS             complement(5976..6842)
                     /locus_tag="ACG80L_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5024203.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6931..7956)
                     /locus_tag="ACG80L_001986"
     CDS             complement(6931..7956)
                     /locus_tag="ACG80L_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5024204.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7974..8648)
                     /locus_tag="ACG80L_001987"
     CDS             complement(7974..8648)
                     /locus_tag="ACG80L_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5024205.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8832..9725)
                     /locus_tag="ACG80L_001988"
     CDS             complement(8832..9725)
                     /locus_tag="ACG80L_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5024206.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9799..11496)
                     /locus_tag="ACG80L_001989"
     CDS             complement(9799..11496)
                     /locus_tag="ACG80L_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5024207.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3421 a         2523 c         1975 g         3601 t
ORIGIN      
        1 taacgtaaaa agaataagtt tttttaaaat aacgtataaa aaagagcata aaacaaagtc
       61 gtttgtttta tgctcttctt ttattatttt gtgcttttta ttttatcaaa tcactttgtt
      121 tttttataaa atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc attcatgtcc
      181 aacgggatcg aaacttgcca cggcatcttt gaaaatatgg agtttgtatc ctagattata
      241 cgcatcgact gctgtatgca acacacagat atctgtacat acgccggtca aataaatatc
      301 tgtgatctgg cgttctcgca aacggatgtc tagatctgtt ccactaaaag cggaataatg
      361 ccgtttgtct atccaataaa cattttcctc ttgctcatag tcttggtata aactttttaa
      421 agagccgtac aattcccgtc cacccgttcc tatcacatta tgcggaggaa ataagcggtt
      481 ctccggatga aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga caaaatcctt
      541 ttgatcaata aacttctttg tatgagcagt cagctttttt tcaattactt gtcctgctgt
      601 cccagtagtc agctttccat cctctgcaac aaaatcatat gtgtagtcaa tcgagatcaa
      661 tgcgttcatc ttagtcctcc ttatacgaga tgtttgacaa attcgatcac catttctgct
      721 gatcgtttgc ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga gtgatctgct
      781 gtgtcgctca ttgcacgtac gatcaaaaat gggatattga actgacgagc tgtttgtcca
      841 acagcggctc cttccatttc gcaagctaat gcttcaggga aattcgttaa gatttctttg
      901 attttatctg gcgagtcgac aaatgtatca cctgtaacga tcaatccttc ttttgcgttc
      961 aaattcgttg cttttgctgc tttaaccatt tcactgcgca aatatgtgct tgcttcataa
     1021 tatagaggca ttcctggtaa ttgtcctggt ttgtagccaa atcctgttgc atctgcatca
     1081 aaataagcta ctttgtctga tataacgatg tcacctactt gtaagccttc accgattccg
     1141 ccagctgaac ctgtattgat caccatgttc acgccgtatt gttggatcaa cagacttgtg
     1201 gtaatagatg ctaagacttt tcctataccg gaacgtacga cgatcacttc gtggttacct
     1261 aatgaacctg aaataaataa agcgcctgcg cgttcccatg ataatggttc gcttaggttc
     1321 tctctcaaaa tttttacttc ttcttccatt gcgccgataa taccgatttt catcttttta
     1381 attccttctt tctatataaa tgcgattgct aaaaaaacca cacataacat taggataaca
     1441 ataataagag atttcatcaa aaaagagttc catttggtcg tcttttcccg ttcgctgatc
     1501 cttgttttcg tgattggttt attctttttg ctttctttac gataaaagct ggcaatcttt
     1561 ttttcttctt gctgataagc tgtttctgct tttcgctgtt tttttaatga ctcgcttgct
     1621 tgtgcttgtt gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt ggccataagc
     1681 cgatccctct actttccttt ggtcaatagt tcccagtatt gaatcgcata aatcgtcttt
     1741 gcatcacaaa tcaactgatc tttcatcgct tgttgcgcct cttcaagagt tagatgatac
     1801 aactccagta cttcgtcttc atcttgtgct aaaggattct cgaccttctc taccccttgt
     1861 gcatgataaa tatgaaggac ttcatttgca aaacctggtg aaagatacat cgaagtaaga
     1921 tggctcagcg acttcgcacg atagccagtt tcttcttcta attctctagc tgctgtcatt
     1981 tcgggatttt gtccttctcc tggatcgatt tttccagctg gaatctccaa aatcactttt
     2041 tctaaaggtt ttcgaaattg tttgacaaga agcagacgat cttgttcgtc aaaagcaatg
     2101 atcccgacac caccaggatg aaataccagt tctctcttcg aagttcctcc atctggcaaa
     2161 cgaacttcgt ctacagcaac atcaataatt tttccgctat agatttcttt gcgactgatt
     2221 gttttttctt caaattgctc attgttcagc atatcttctc ctgctttctt tgtaatcata
     2281 actgcttttt tttcattttt caatttttct caaaaactcc ttattagttt accatagata
     2341 aaccttagat tttctctaaa tatagaaaat ccttctttag tccgatactc tttctagtag
     2401 tccgtatgct aaaataagta tgtaatcgaa taacgaaaag aattcaatac tcaacgataa
     2461 atgacatgga gggtttatta tgataaaacg aaattggccg tggcttctag cattggtctt
     2521 gctatttctc tttgtccgaa acaatagttt ttcttttgtt ctaggacttt tattgatagg
     2581 tgtagtttta gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa aacaagaaga
     2641 aattcctgca ctgactgacg aacttgaaac tcactatgaa aaaagcggta tgacagcgaa
     2701 tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca atcaattgca
     2761 aatgaatatg cagcagacag ccaaacttaa agcaatcgat ctccgccatg atccagtcaa
     2821 agcagcgaaa gctttattca aagaattggt gaaagaacca aaccgactgc atgaagcaag
     2881 tcagtttctt tatacgcatc tgccgaatct agttgatttg acaaataagt ataatgagat
     2941 caatgaccat gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa gcgcgcagat
     3001 catcgaccag ctgtcagctt taatcgcaca agattatcaa aaattcgttg ctgaggatct
     3061 agatgatatc gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg ataataaata
     3121 tgaaccagaa caaaaggatt aactcttttg ttcataaaag aagactcgga acattcagat
     3181 gatgtctggc tgtttcgagt actttttcta aagtagagac attcatgtaa gaatcgagaa
     3241 cagattcaac aaggaggaat tttttatgga tcataaaccg actgaaacaa aagatgttac
     3301 accagttaac gatacacttg aagatttatt gaacaacccg ttttcaactc caacagatac
     3361 gctgaccact tcacaacaag cggagattga tcagctccaa gagcaacaga cagctgctcg
     3421 tttaattgac aaactaccgg cggaacgcca agaacaagct aaacagctag cagcaaaaat
     3481 cgatgctaat gatgcacagt ctgtcatcag ctacggatcg gctgcccaag caaaattaag
     3541 tgaattctca caatcgatgt tgaatcatgt gcaagcacaa gatattggtc ctgtaggaga
     3601 ttccttaact gaactgatgt atcgtctcca agaagccaat cctgacgaac tgcgtgccgg
     3661 agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct atgaagtgac
     3721 agcaaaatat caaaagattg gtgcacaaat tgacaagatt tcagtgaaat tagataaaga
     3781 aaaagacggt ctgctaaaag ataatctgat gttagaacag ctttaccaga aaaacaaaga
     3841 ctactttgat gcactgaata tttatatcgc tgctggagaa ttgaaaatgg aagaacttca
     3901 aacaacgatc attccagaag cgatgaaaag agcagaagaa actggcgatc agatggatgt
     3961 tcagattgcc aatgattaca cgcaattcct agatcgtctg gataaacgta cgcatgatct
     4021 tcgcttagct agacagatca cgatccagca agcaccacaa atccgtttga tccaaaacac
     4081 aaatcaggcc ttggctgaaa agatccaagc atctatcgct acagccattc cgctgtggaa
     4141 aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg
     4201 ccaagtctct gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc
     4261 agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac
     4321 cactcaaaat gatttgatcg aaacaatcca agagacattg cgtatccaaa aagaaggaaa
     4381 agaaaaacgt cgtcatgcag aagtagagct tggacacatg gaagaagatc tgaaacaaaa
     4441 actgttggat ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa
     4501 cagtttatta caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga
     4561 gtctatttct cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca
     4621 ataaccatta cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca
     4681 atacaagtca agatggagac gattggcaat agcggaacga atggcacttt gaattctcca
     4741 gcttttggtt tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa
     4801 taggcgagcg ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca
     4861 aagaatacag ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga
     4921 tcgatttctg tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga
     4981 gcaagtgaat acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg
     5041 atcaccgcgc ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg
     5101 actcctagtt cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg
     5161 acagtagcaa tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg
     5221 acttcctcgg ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct
     5281 gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta
     5341 acgaaaaata ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg
     5401 gcattattca aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt gacgaatatc
     5461 actacgagca ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt
     5521 aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat
     5581 ccggaagcaa cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc
     5641 caggcagcaa attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc
     5701 actcgcgaag caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag
     5761 acaaatgaaa gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca
     5821 aatattcccg taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt
     5881 tcttttttca tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt
     5941 gaagttcctc cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa
     6001 aggataccac ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc
     6061 gtttttcttt cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt
     6121 gaaagtgaga agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt
     6181 cctaagcccc ataataatcc acagatcatg tttttccaca caaactgatc gactttaact
     6241 ttacggaaag caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt
     6301 gacatcgcat ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta
     6361 gagaagatca atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt
     6421 tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca
     6481 ataataccgt atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca
     6541 atcgtattca aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg
     6601 tacttcattc catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat
     6661 ccgatcaaga aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa
     6721 aacagaaatg caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta
     6781 ccacttacta aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc
     6841 ataaccaatc tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca
     6901 caagcacttt ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat
     6961 tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat
     7021 acctaatttt tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt
     7081 gctaagtcga ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc
     7141 tgggataccg atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat
     7201 gctcacaatg atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt
     7261 gaagataaaa gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc
     7321 gtagcgaata tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat
     7381 tccttttagt ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata
     7441 atacaaaact tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata
     7501 cttttgatcg tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg
     7561 cactttgatt tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat
     7621 tgctgattgc attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag
     7681 tttttcttct tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat
     7741 gtcggctaat tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat
     7801 cagaaagacg ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt
     7861 tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa
     7921 tgtccactga tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga
     7981 acgatatatc cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt
     8041 tttcggcgta accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca
     8101 tcccacagtg ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa
     8161 ataaataata gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata
     8221 cttccgttgt caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct
     8281 gtgtagttgt aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg
     8341 aaatgttttg tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc
     8401 gtattcctgc tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac
     8461 caataatagc catccaaaac gggaagatta atatctagta atacaagctg tggttcttct
     8521 ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat
     8581 ttctcaagtt cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt
     8641 tttgccataa aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg
     8701 tgaaacaagt tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa
     8761 tatgcgattt aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa
     8821 taattggtct attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa
     8881 caccaataac cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga
     8941 taaagatggt atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact
     9001 cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt
     9061 gtgactagat cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca
     9121 attttctcaa cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca
     9181 tcttgctcca tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg
     9241 gtaatcacgg aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc
     9301 gctcctctat ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt
     9361 ttcggaatat agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg
     9421 acatttctaa ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat
     9481 gtatgagcaa gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct
     9541 tctttatcgc gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg
     9601 ctgagagcat ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact
     9661 gccataaata attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt
     9721 ttcattttct tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct
     9781 gcagcttatt tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta
     9841 cgctccaatc atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt
     9901 agcaggaagt gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc
     9961 tacgatctga atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc
    10021 ctcacctgtt ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc
    10081 ataatctaac ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg
    10141 tccgcttgtt gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc
    10201 ccctttataa aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc
    10261 tttaagcaat tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac
    10321 tttctctggc ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat
    10381 aaccgctgtg ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata
    10441 cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc
    10501 tactccattt tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat
    10561 ataatcttgt ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata
    10621 ataagttaga ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc
    10681 gttttcaagg acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt
    10741 ttttcctctt tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat
    10801 cgttacttgt ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc
    10861 gtttttcgga tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca
    10921 cacgccatcc accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat
    10981 tctggaaata tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga
    11041 aggtgcttct tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt
    11101 ttgtttgtca atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg
    11161 aatccattca atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt
    11221 atctcgaatc aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg
    11281 aatattttct tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac
    11341 agatgagaaa ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc
    11401 ttcggctttg gctagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag
    11461 tacactcatt cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta
//
LOCUS       DAZUMB010000086        11137 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000086 DAZUMB010000000
VERSION     DAZUMB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.088"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG80L_001990"
     CDS             6..305
                     /locus_tag="ACG80L_001990"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5024208.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG80L_001991"
     CDS             378..1739
                     /locus_tag="ACG80L_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5024209.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG80L_001992"
     CDS             1761..2021
                     /locus_tag="ACG80L_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024210.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG80L_001993"
     CDS             2035..2787
                     /locus_tag="ACG80L_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS5024211.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG80L_001994"
     CDS             complement(2881..3486)
                     /locus_tag="ACG80L_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5024212.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG80L_001995"
     CDS             complement(3539..4513)
                     /locus_tag="ACG80L_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS5024213.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG80L_001996"
     CDS             complement(4891..5610)
                     /locus_tag="ACG80L_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5024214.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG80L_001997"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG80L_001997"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5024215.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG80L_001998"
     CDS             7161..8558
                     /locus_tag="ACG80L_001998"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5024216.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG80L_001999"
     CDS             8639..9079
                     /locus_tag="ACG80L_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5024217.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG80L_002000"
     CDS             9227..9553
                     /locus_tag="ACG80L_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5024218.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG80L_002001"
     CDS             9554..10303
                     /locus_tag="ACG80L_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS5024219.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG80L_002002"
     CDS             complement(10383..10838)
                     /locus_tag="ACG80L_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5024220.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3533 a         1949 c         2290 g         3365 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttact
//
LOCUS       DAZUMB010000087        10708 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000087 DAZUMB010000000
VERSION     DAZUMB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.089"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1263
                     /gene="lysA"
                     /locus_tag="ACG80L_002003"
     CDS             4..1263
                     /gene="lysA"
                     /locus_tag="ACG80L_002003"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS5024221.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1250..2050
                     /gene="dapF"
                     /locus_tag="ACG80L_002004"
     CDS             1250..2050
                     /gene="dapF"
                     /locus_tag="ACG80L_002004"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS5024222.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2053..2934
                     /gene="dapA"
                     /locus_tag="ACG80L_002005"
     CDS             2053..2934
                     /gene="dapA"
                     /locus_tag="ACG80L_002005"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS5024223.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2931..3647
                     /gene="dapB"
                     /locus_tag="ACG80L_002006"
     CDS             2931..3647
                     /gene="dapB"
                     /locus_tag="ACG80L_002006"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS5024224.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3653..4348
                     /gene="dapD"
                     /locus_tag="ACG80L_002007"
     CDS             3653..4348
                     /gene="dapD"
                     /locus_tag="ACG80L_002007"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS5024225.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4364..5365
                     /locus_tag="ACG80L_002008"
     CDS             4364..5365
                     /locus_tag="ACG80L_002008"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS5024226.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5362..6723
                     /locus_tag="ACG80L_002009"
     CDS             5362..6723
                     /locus_tag="ACG80L_002009"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS5024227.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6683..7834
                     /locus_tag="ACG80L_002010"
     CDS             6683..7834
                     /locus_tag="ACG80L_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5024228.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7809..8978
                     /locus_tag="ACG80L_002011"
     CDS             7809..8978
                     /locus_tag="ACG80L_002011"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5024229.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9153..10532
                     /locus_tag="ACG80L_002012"
     CDS             9153..10532
                     /locus_tag="ACG80L_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5024230.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3535 a         1884 c         2415 g         2874 t
ORIGIN      
        1 aaaatgacag aattgataat tggcggagtc acagcaagca cattggcaga aaagtataag
       61 acacctctgt atgtgtatga tgaagaaaag atgagacaaa cgatgctgtc attcaagcga
      121 ggctttgtat cgaaacgttt tgatacaaaa gtgctttacg cctctaaagc attccaaacg
      181 attgagatgc tgcatttagt tcgtgaatat ggattcgggc tagatgttgt cagtggtggg
      241 gaaatctata ccgctttgca agctgatttt cctagagata ctatctactt ccatggtaac
      301 aacaagacgc cagaggaact gatctatgca cttgaaaatg atttgttgca tattgttgcc
      361 gataatttga tggaagttga attattagca gaattaagcc aacgctattt aaaaaaaatc
      421 tgtatcatgc ttcggttgaa tgtcggagta gaagcacata ctcatgaata tatcgtcact
      481 gcgcatattg attcaaaatt cggcatgagc tatgaaagta aagagtgcca acaagccctt
      541 cagttgataa aagaaagcag ctatctagag ttagaaggat ttcatgctca tatcgggtca
      601 caaatttttg atgttactgc ttggttagct gaaatcgaca aattgacgaa ttacttaacc
      661 gattttgacc aacctctttc cttgaatctt ggcggagggt ttgggattcg ttatacacaa
      721 aatgatcagc cgatcccagt cgaagaggca ctaaaaaaac tgattgaata tacggaagaa
      781 gctttggaaa aacgaaaact gaccattcgt caattactga tcgaaccggg tagaagtatt
      841 gttggagaag ctggtacaac actatacacg attggtttca tcaaaaagac accacataaa
      901 aaatattact ttgtagatgg cgggatgaca gataatatcc gtccagctct gtatcaggca
      961 gaatacgatt gtgatttagc agcaaaactg tcggaagaaa aaacagagaa agtgacgatt
     1021 gcaggaaaaa tgtgtgaatc aggcgatgtg gtaattaaag aagcttattt gccgaaagca
     1081 gaatcgggtg atcttttagc tgtttattcc acgggagctt atggatactc gatgagcagc
     1141 aactacaatc gagcgacacg accagcagtt gtttttgtca ataatggcca atcaagactg
     1201 attgttcgca gacaagcatt ttctgatcta ctaagggggg aagtttccta tgatccttca
     1261 taaatataat ggctgcggaa atgattttat cttattagat tacgcagaag acacagatta
     1321 tagtcaatta gccgcttctt tatgcacaaa agaaaaatat gatacagacg gattgatcgc
     1381 cgtaaaaatc gatccgttgg aaatgatcta ttacaacaaa gatggcagcc gcgcgccgat
     1441 gtgcggaaat ggcattcgct gttttgctcg atacgtaaat gatcagaaca tgatgaatga
     1501 acaggaattc aacgtagaga cattagcagg cacaagaaaa attacggtgt tggatccctc
     1561 acctttttat tgcagcgtag atatgggagt gccagatgat tctccggaaa aaatcaaagc
     1621 taatcaaact tatccaataa aaaaccagga aatagagatc aatggtgaaa aagtaacgat
     1681 cactagttta tttttaggaa cgattcatac agttgttttt gttgaaaatg cgcgtgagga
     1741 agtctggaga aaaagaggtg aactgatctg tcatcatcct ttgttcaaag aaaaaaccaa
     1801 tgtcaacttt gtagaagtag tcaatgaaca agaattgatc gtccgtacct atgaaagagg
     1861 agtaggttgg acacttgctt gcggtacagg ttgctgtgcc tcttatgtgg ttgctaagga
     1921 aactgggaaa atttcagcag atcaggtagt tgttcacttg gaacaaggag acttgatgat
     1981 ctctggtgag gatgcgatac aaatggctgg cccagcagtt catgaatgga caagaatatt
     2041 ggaggaatga agatgataaa aggttcgatc gtggcgctga tcacgccgat gaatgaagag
     2101 ggatcagtag attatgctgg tttggaaaaa ttgatccaat tccatttaga tgaacaaaca
     2161 gatggattat tagttttagg tacaacagga gaatcttcta cgctcacaca atctgaggag
     2221 gagcagatcc ttcaactgac agtaaagaaa gtggctggcc gtgtgccggt gattgcagga
     2281 gctggaacga ataatacgaa agaaacgatt gaaaaagcga agcattttgc ttctttgggg
     2341 gcagacgcac ttttggtcat caccccttac tacaacaaaa caagtgatgc aggactagct
     2401 gctcatttta cagcaattgc agaagcctca ccgattccgc ttattttgta taatgttcct
     2461 tctagaacag gcatgtcgat tccaatccat gtattagtaa atttagcaga acatcctaat
     2521 atcattggtt tgaaagaagc ctcaggagat atggcttatg tgatggatgc cgctcggttg
     2581 attggagaag aattcttcct ttacagtgga aatgatgatt tgatcctacc agtaatgagt
     2641 gtaggaggaa gcggtgtaat aagtgtatgg gcgaatatcc aacctaaaat agtccatgaa
     2701 ttagtaaaag atacgcagga cggaagatgg cagcaagcaa aagaaaagca attaaatgca
     2761 cttgaactga tccatgcctt gttcagtgag accaatccta ttcctgtcaa agcagccatg
     2821 tcattattag acttaccttc cggacctcta cgcctgcctt tagtttctct ttcaaaagag
     2881 aaaaagaaac aattagctca gcttttgtta aaagaaagga cggttgaaaa atgaaaatcg
     2941 gactgattgg cagtggacag atgggaaata gagtcgaaga agttgcacaa gaaagaggag
     3001 atacggtcct tcttttacgg acggcaccta aagaagcaac tacgcttgct tttacggaac
     3061 tgccagaact gcttattgat ttttctcatc cagataattt agagaaaatc ctttcgtata
     3121 gcttatcata tcaacttccg ttagtcattg gcacgacagg ttatacacaa tcacagttcc
     3181 aagaaataaa agaacatgcc aaacatgtac ctgtgatgta tagtgctaat ttttcgttcg
     3241 gcatcatggt gatgaatcaa ctaatcaagc aagcagctgc aatgttgcaa ggctggcaga
     3301 tcgaactgat tgaaaaacat catagtcgta aaaaagatgc accatctggt actgcaaata
     3361 tgctgcttca aacaatccaa gaagtgcaaa agctgcagcc cgtatacaac tggcaaggga
     3421 aaaaaagaga agaaaaccaa attggtgtac acagtattcg tgcaggttct ttacctggtg
     3481 aacatgatgt attgtttgct gctactgatg aaatatttac gatcaaacat gaatcttttt
     3541 ctaatcgaat ctttgctgca ggcgcagtag aagcagcgga ctggttgaaa gatcagtcac
     3601 caggttatta caaattagaa gatttattaa tggacaaagg ggtgtaaaaa acgtggaagc
     3661 tcaagaaatc atccgctaca ttcaaacggc atctaaaaag acgcctgtga aagtctatct
     3721 gaatgtaacc gagccaatcg aatttaaaga aagtaaagtt tttggtgaag gaagcagctt
     3781 cgttgttttt ggtgactatg agacgattgc tccggtttta gaagctgaat cagaaaaaat
     3841 cattgaaatc gaaatcgaaa ctgctgcaag atacagcgca gttcctttgc tagatattaa
     3901 aaaaattaat gctcgtatcg aaccaggtgc tattatcaga gatcaagtgt caatcggcaa
     3961 caatgccgtg atcatgatgg gagcgattat caatattggt gcagtaatcg gtgaaaacac
     4021 gatgattgat atgggtgctg tcttaggtgg acgagcgaca gtgggcaaaa actgccatat
     4081 tggtgcagga gctgtcttag caggtgtgat cgaaccagca tcggccaaac cggttatcgt
     4141 agaagacggc gtactggtag gtgcgaatgc agtgattgtt gaaggtgtcc atatcggaaa
     4201 agatgcagtc gtggcagcag gagcagtggt tttagaagat gtggcagcag aaactgttgt
     4261 cggtggaata cctgcacgtg tattaaaaat aagtgatgat cagacaaaag aaaatacagc
     4321 attgattgct gcgttacgcg aattatagag gagatggaga aaaatgcgcg aaatagatac
     4381 ctgtattgtg ggggcaaccg gattagtcgg ccaaacgatg ctcaaagtgt tagaagaata
     4441 tgattttcca gtaggacgat tgaaattatt agcttcaaaa cgttcagccg gtaaagtttg
     4501 tgtattcaag aaaaagaaac acactgtaca ggaattgaca gaaacttcat ttgaaggata
     4561 tgatttagcg ctattttctg caggtgcttc tgtatccaaa gagtttgcgc caatcgcacg
     4621 aagtcaggga ctatttgtca tagataattc ctcagcatgg cgggaagatt cggatattcc
     4681 attaatcgtt ccagaggtca atctggaaga ctatacattg aatcggctga ttgcaaatcc
     4741 aaattgttca accattcaag cggtacttcc gcttaaagct ttacaagaag cctttggtat
     4801 ccagcgagtc aattattcga cctatcaagc agtttctggt tccgggcaaa aaggcattga
     4861 agatcttcat cgtacgcaaa atgacgagga accagttttg tatccttata atattagcca
     4921 aacggtgatt ccggaaatcg atcagccttt ggaaaatggg tatacaaaag aagaacagaa
     4981 aatgataaat gagacccgta agattctgca tcagcctgct ttgccagtat cagcaacctg
     5041 cgtacgtgtg ccaatcgaaa atggacatgg agtctctgtg gcagttcaac tgcaaaaaga
     5101 gtttacgacg gaagaaatta gagagtgtct cagccagttt ccagggattc aattagaaga
     5161 tgatctagca gcacataaat atccgacgag cattcttgct agaggaacag atatggttta
     5221 tgttgggagg atcagaaaag acacttcttt agagaacgga ttgttgttct atacgactgc
     5281 cgataacata cgcaaaggag ccgctgcgaa tgccgtacag attgctgcag ctttaatgca
     5341 gaaaatagcg gaggtggcaa gatgagtaat acgctgaaag tagcgaaatt cggcggaaca
     5401 tcgatgtctc atgaagagca attcaaaaaa gtccgtacaa ttgttctttc agatcccaat
     5461 cgccaagtga tcgttgtttc tgctttaggc aaacgtacgt cagatgatga caaagtaaca
     5521 gacttacttt atttgattta tgcttacctt cagcatcatg ttgattggac acccctttgg
     5581 aaaaggattt gtgatcgttt cattcaagta agagatgttc ttgccctgca gttacctatt
     5641 gaagatgaac tagcacgcat acagaaacaa ctggaagacg aaaagataac tgaagattat
     5701 ttagtaagta gaggtgaata tctgactgct caactgatgg ctgagtatct tggttaccag
     5761 tttattgacg caaaagacct gattttcttt gactatgatg ggaaaatcga tcaggaaaaa
     5821 acagcacagg aaatcacaaa agcatttaaa agatatccga aaatggttgt tccagggttt
     5881 tacggggcga accctgcagg aaaagaaaaa ttattgggcc gtgggggaag cgacattacc
     5941 ggcgcgatac tggcgaagat gttgcaagcg gaaagttatg agaattggac ggatgtttca
     6001 ggaatcatga tggctgatcc gcgaatcatc gatcatccta aaatcatcga ggaaatctct
     6061 tttagagaac tgagagagat ggcttatatg ggggctaacg ttcttcatga agcagctgtt
     6121 ttcccagttc aagaggctga tatccccatc caaataaaaa ataccaatga tcctttcgct
     6181 caagggacaa aaatatcgaa tcacgaaata gaaaaggaaa atggactgac aggaatagct
     6241 ggacgcaagg attttctttc catcacttta ttcaaacgtc atatgtccga tgaaatcggg
     6301 tttatctgga aagcaatgag catttttgct aaacatgaga tatcaatcga acatattcct
     6361 tcagggatcg acaatatcgg agtgatcgtc tcagcagaag cgattgccga taagttattt
     6421 ctcatcacga aagaattaaa agaaacactt ggcgtggaag aaatcgtcgt gatcgaagac
     6481 ttagctttga tctctgtcgt tgggggtccg cataaagaat tgattggatt atctgggaaa
     6541 gtgttatcga tattgaacaa attagaaatt cgcacatcga tcttatcaca aggagcacag
     6601 gagttgaatc tgatcatcgg tgtaccgaat aatcaatatg aaacagtagt gaagggtatc
     6661 tatgaaggga tggtgaatac ggatgctgaa agaatatcga atggcactcc atcaaattcc
     6721 tgaaatcggg tttcatgaat tcaagacaaa aaaatattta tatgaacaag taaaaaacag
     6781 cggggccgtt atccatgaaa tcggcgaaac aggactactc ctttattttg ataatcagca
     6841 agaagaaacg attgcttttc gaacagatat cgatgcactg ccagtcaaag aagaaacgaa
     6901 tctctctttt gcttctaagc atgaaggtta tatgcatgca tgtggtcatg acggacatat
     6961 ggcgatgctt ttagggttga ctgactttat aactaaacac attgaggatc ttatttataa
     7021 tgtagtactg atctatcagc catcagaaga aatttccggt ggcgcacaaa gcgttattca
     7081 gtcaggattg ttggataaat atgaagtaca agcaatattt ggctttcatc tatggcctgg
     7141 actagctaag ggagaagttt tctctcgacc aggacctttg atggcacaaa gcagtgaaac
     7201 agacattatc attaaaggcc agtctgcaca tattgcttct agtgaaaaag gaatcgatag
     7261 tctggaggca gctgtacgat ttttgaagaa tgtttatgat tttgaccggc aattaccggc
     7321 agagtggaat catctaatga aatttggcca aatccaaggt ggaacgatcc gcaatgtttt
     7381 agctggagaa gttattgtat ccggaagtat ccgttcttac agttcagata tgcaaaatta
     7441 tttgaagagg ggattagacc atttagcaga acagtttcac tctacagcta ctatttcttt
     7501 tcgctataat gacggctatc cagctgtaat caaccataga ggcttatatg cagcactgtt
     7561 agattcaaac aaactacatg aattagatgc tccagttctt caagccgaag attttggcat
     7621 atatacgcaa caatatccat gtgtattttt ctttttaggt gtcggtgata cccctgcttt
     7681 acatgatccg acgttcgatt ttgatatgga tgttttagaa aatggtgtag aatggtacaa
     7741 aacgattctt tttacagaaa atcttcttgt aaaaatcgat agcagttcaa aagaggagcg
     7801 agtaaataat gatgaattta tccaatcgat ttagtcctag gctttctaga atagaagtat
     7861 caaaaatacg acaattcgat cagcaaatat cgtcgatacc agatgtgatc aaactcaccc
     7921 tgggagaacc ggattttcca actcctgaac atgtcaagca agcaggaata gcagcgattg
     7981 aagaagactt ttctcactat accggaatga gaggattaga ggaactgcgg gaagcggctt
     8041 gtatatttca gcaacagcgt tatggattaa cttatgatcc gcaaacggaa gttttgacga
     8101 cagttggtgc aacggaagcg attgcctctg cgttgttatc tgttttggaa gaaggagaca
     8161 aggttcttat tccagcgcct gcttattcag gatatcagcc attagtggaa ctagctggtg
     8221 ctgaattgat cccgatagat acttctgaca cagggtttgt ctgtcagccg gaacaatttg
     8281 aacgagcatt tgaacaatat ggatcagctg tcaaagcagt cattttaaac tatcctaaca
     8341 atcctactgg gacgacattg tctgcaaaac aattgaaagc aatcgctgag gttttaaaaa
     8401 aatatccggt atttgttata agtgatgaag tatatgctga attgacttat agtggaacgc
     8461 atatgtccat agctacttat cttccagaac aaacaatcgt gataagcggg ttatccaagt
     8521 cacatgcgat gactggttgg cgagtgggct ttatttttgc tcaaaaagcc cttattgacg
     8581 aactaattaa agtccatcaa tatctggtga ctgccgctac aacgatgtcg caaaaagctg
     8641 cggctgaagc gctaataaat ggggtgaaag atagcgagaa tatgaaagag caatatcaaa
     8701 cacgaagaga ctatctggtc cagcaattgg cgcctttagg atttgaagtg acccagccaa
     8761 acggtgcatt ttacttgttt tgcaaactcc cagctgccat tcaaactgac tcttggcagt
     8821 tttgtgtaga attagcggaa caggcaaaag ttgcgtgtat cccaggaatt gcatttggcc
     8881 cagaaggaga aggttatatc cggatttctt atgctagcag catggaaaaa ttacatgagg
     8941 cctgcaaacg gatcaaagac tttttaacag aacagtgatt catagatttt atcaaaaaaa
     9001 caatcgttaa ttttgcggta tattctgcaa ggagacgatt gttttttaaa tttagttcaa
     9061 atgaacgcgt ttcttttgat tcataactaa tgtaatgtta agtttaaatt gtttagaatg
     9121 attttaaaag aatattcaag gaggtttcat caatggaaaa aatcaaagtt gtcattgtag
     9181 gtgcatctca tggagggcat caatcgatct tggaactatt gacacgatac ggtgaaaatg
     9241 tagatatcac gctatttgaa gcaggagact atatttcctt catgtcatgt ggtatggagc
     9301 tgtatttaga agacaaggta acagatgtaa atgatgtacg taatttcaga ccagaaaact
     9361 ttccgcaatc caatgttcat attttaaata atcacgaagt aaaaacgatc gatgccgata
     9421 aaaaaacagt aacggttact agcgcatcag ataacgaaac aaaagaatat ccttatgaca
     9481 aattgatttt gagttctggt gtcaaaccaa acaatctgcc agtaccagga gcagatctag
     9541 aaaatgtcta cttgatgcgt ggatacaatt gggcaaccaa aatcaaagaa aaattaaatg
     9601 atccggctat taaaaaaatc gctgtcgtag gtgcgggtta cattgggatc gaagcagcag
     9661 aagcaagccg aaaagctggg aaagaagtcg tattgcttga tgtgatcgat agacctttag
     9721 gcacttattt agatgctgag atgacagata ttttagaaca acacctaaat gaaaacggta
     9781 ttgaagtttt gacaaatgca aaaatcgaag cttttactgg taacggaaaa gttgaggcaa
     9841 tcaaaacaag tgaaaaagaa atcccagcag atctagtcat tcaagctgcc ggtgtgaagg
     9901 caaatacaga atggttaaaa gggatcgtcg acttagatga acgtggctgg atccaaacaa
     9961 atgaatactt gcaaacgaat cttccagatg tgtatgcagt aggagatgct acacttgctt
    10021 attctatccc agcacgcaaa aaaatgccga tcgctttagc gactgttgca cgtagagaag
    10081 cacgttatgt agtaaaacat ctttttgaag aagtaccaaa caaaccgttc ggcggagtcg
    10141 taggttcctc tgcattaagt gtctttgact atcattttgc agcaagcgga ttgaatagct
    10201 ttacagcagc aaaagcagga gtagatgtac gagtgtctta ttatgaagat acgttacgac
    10261 caaaatatgt accagaagaa caaggaaatc caaaagtgtc catccaatta ttctcggatc
    10321 caatgaccca tcaattgcta ggaggagcag tattatctac ctatgatgtt acaggtcaag
    10381 gaaatgtatt ggcactagcg atccaaaaca aactgacttt agaagaacta gcagaagctg
    10441 acttcttctt ccagccaggc tttgaccgac aatggagcat gttgaatcta gccgctcaac
    10501 aagcgttagg agaagaacca tttgtagaat aagaaaacga aatatttgaa aagaggttgt
    10561 gaaaaagctg ccttgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt
    10621 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attttcaaaa
    10681 aagggactgt gacacgattt gtgtaccg
//
LOCUS       DAZUMB010000088        10498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000088 DAZUMB010000000
VERSION     DAZUMB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.090"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG80L_002013"
     CDS             34..657
                     /locus_tag="ACG80L_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024231.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG80L_002014"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG80L_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5024232.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG80L_002015"
     CDS             2153..3034
                     /locus_tag="ACG80L_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5024233.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG80L_002016"
     CDS             3037..3807
                     /locus_tag="ACG80L_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024234.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG80L_002017"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG80L_002017"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS5024235.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG80L_002018"
     CDS             5118..6281
                     /locus_tag="ACG80L_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5024236.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG80L_002019"
     CDS             6398..6808
                     /locus_tag="ACG80L_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5024237.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG80L_002020"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG80L_002020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5024238.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACG80L_002021"
     CDS             9381..9665
                     /locus_tag="ACG80L_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024239.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACG80L_002022"
     CDS             9825..10061
                     /locus_tag="ACG80L_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5024240.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACG80L_002023"
     CDS             10058..10456
                     /locus_tag="ACG80L_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5024241.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3660 a         1766 c         2224 g         2848 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcat
//
LOCUS       DAZUMB010000089        10432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000089 DAZUMB010000000
VERSION     DAZUMB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.091"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..602)
                     /locus_tag="ACG80L_002024"
     CDS             complement(117..602)
                     /locus_tag="ACG80L_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS5024242.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    692..1037
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1082..1723
                     /locus_tag="ACG80L_002025"
     CDS             1082..1723
                     /locus_tag="ACG80L_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5024243.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1742..2377
                     /locus_tag="ACG80L_002026"
     CDS             1742..2377
                     /locus_tag="ACG80L_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5024244.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2395..3210
                     /locus_tag="ACG80L_002027"
     CDS             2395..3210
                     /locus_tag="ACG80L_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5024245.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3529..4965
                     /locus_tag="ACG80L_002028"
     CDS             3529..4965
                     /locus_tag="ACG80L_002028"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5024246.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5154..7601
                     /locus_tag="ACG80L_002029"
     CDS             5154..7601
                     /locus_tag="ACG80L_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS5024247.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7831..8898
                     /gene="rlmN"
                     /locus_tag="ACG80L_002030"
     CDS             7831..8898
                     /gene="rlmN"
                     /locus_tag="ACG80L_002030"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS5024248.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8888..9094
                     /locus_tag="ACG80L_002031"
     CDS             8888..9094
                     /locus_tag="ACG80L_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024249.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9091..10194)
                     /locus_tag="ACG80L_002032"
     CDS             complement(9091..10194)
                     /locus_tag="ACG80L_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5024250.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3437 a         1793 c         2270 g         2932 t
ORIGIN      
        1 ttcagcgttc atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt
       61 tgtgctaaat aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac
      121 aaattttcat aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa
      181 tccataatca acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca
      241 atagcagcat cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga
      301 gtcactgtac ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat
      361 aagcgctttt cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc
      421 aagatgacaa gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc
      481 ggaccaggta gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt
      541 tgaaaagaga tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc
      601 atcgcgcatc accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa
      661 ataatcatgt aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt
      721 taggtgtttt tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca
      781 tcttggagtc gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc
      841 ggtcgttgct agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt
      901 ggaaccacgt gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga
      961 acggcaacga ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg
     1021 tataatacgt cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa
     1081 gatggagtat ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa
     1141 attattttta tttaccttga ttggctcatt accattaggg attgttttag cattaggatt
     1201 gtctttgcat tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg
     1261 aacaccgctg ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt
     1321 ttttgatcgt ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc
     1381 agagatcttt cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt
     1441 tttgcaatta agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat
     1501 tgttttacct tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta
     1561 tatattaggt ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac
     1621 tttactgcct ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat
     1681 cataatgaaa caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa
     1741 tatgattgaa ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt
     1801 gaatctaatc attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa
     1861 aaccacgctt ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt
     1921 agacggtgta ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt
     1981 attccaagac ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc
     2041 aaaaatggta ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc
     2101 ttcattaggg attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa
     2161 acagcgggta gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga
     2221 accaacaagt gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt
     2281 gaaagaagat ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt
     2341 cggtgatcaa ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa
     2401 aaaaccagtt ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac
     2461 aaaaaaactt cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt
     2521 ttagacgata catttgttcc tatgggattt agagataaag acgggaagct cacaggattt
     2581 gatattgatt tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc
     2641 attgactggt caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac
     2701 ggctattcca aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa
     2761 aacgatcaag tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat
     2821 aataagagat taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca
     2881 gaagtattaa aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa
     2941 gcattgatcg atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca
     3001 gactattatt tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat
     3061 gagagtgagg attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc
     3121 aatgaagccc tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg
     3181 tttggcgaag atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc
     3241 acagtccctt tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga
     3301 atggcaggct cacaacctct tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc
     3361 caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact
     3421 aagcgacttt ttcacaacct cctttacata attttatttt gttgtggaag gggtttcgtg
     3481 ttattctaaa agaggaacga ttgaaggtta taggaaagga agagatgaat ggaacacaaa
     3541 gaacttacct catttccaaa agattttctg tggggctctg cttctgcagc gtatcaagta
     3601 gaaggagctt ggcaagaaga tgggaaagga gaatcagtct gggatcgatt tgtacggata
     3661 cctggaaaga catttaaagg aacgaacggt gatttggcag tagaccacta tcatcgttat
     3721 aaagaagata ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct
     3781 tggacacgga tttttcctaa tggacgagga gaagtcaatc aagctgggct cgctttttat
     3841 gagagattga tcgacgaatt gattgaaaac gatattgaac ctatgttgac tttatatcat
     3901 tgggatcttc cacaagcatt gcaagatgaa tataatggtt gggaatcacg tcagatcatt
     3961 gaagatttca ctcattatgc agaaacatta tttgaggcat tccgcgggaa agtccattac
     4021 tggattagtt tgaatgaaca aaatatcttc acttcattag gttatttgtt agcagcacat
     4081 ccgccaggtg tgacagaccc gaaacgaatg tatgaagtta accatattgc taatttagca
     4141 aatgcatctg tcatcaataa atttcacgag ttagaaatgc ctggaaaaat cggaccaagc
     4201 tttgcgtata cgccaaatta tccaattgac agtaatcctg aaaatgttct ggctgctgaa
     4261 aatgcagaag acctgatggc caattattgg atggatgtct atatgtgggg aaaatatccg
     4321 attgctgcca tgaggttctt agaagaaaaa ggatgggcac caacgattga agctggcgat
     4381 gcagaattgc tagaatctgc taaaccagat tttcttggga tcaattacta tcaaacggcg
     4441 acgaatgcat tcaatccatt aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa
     4501 aaaggttcat cagaagaaac tggagttcct ggaatgtata aaaaagttga gaatccattt
     4561 gtcgaacgaa caaattggga ctgggagatt gacccagaag gattaaggat cggactgcgt
     4621 cgtatcacaa gccgttaccg catgccggta ctgattacgg aaaatggctt aggagagtat
     4681 gacaagttga cagaagataa acaaatccat gacgactatc gaatcaatta cttacaaagc
     4741 cacgtcaaag ccatcaaaga agcaatttcg gatggagcag aagtcctagg ctattgcacc
     4801 tggtcttata cagatttgct tagctggctg aacggttatc aaaagcgata tggatttgta
     4861 tatgttgatc aagacgaaac acaagaagga tctttggaac gatataagaa agacagttat
     4921 tactggtacc aaaaactgat tgaagaaaat ggagagaacc tttagttcac atacttaaaa
     4981 caagaaggta cggctggata tggccgtgcc ttttcttgat aaaatcattt agaaattaag
     5041 aaaaactgta aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt
     5101 agttccctta cttttttttg tataatgaaa cttgatgaaa tgagggataa taaatggcac
     5161 aaaagaaacg gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc
     5221 agcaggaaca aatgctgacg attggtgttg ggtttgcctt gattttattt gctgtcttcg
     5281 gctttttaaa attaggtttt ttaggaatat tgattgcaaa cggctttcgg atcattgcag
     5341 gaaatactta ccaaatcctc tgtcttttat tagccgtatt aggattttgg attgtcatta
     5401 agaatacaga gttttcaatt ggtaaaaacc gacgctggtt tggcggcata ctcttttatt
     5461 tcggtatctt attattgtta catgcccatt tatttggcaa actccatact ggggaaccaa
     5521 atatcatggg gacgacatgg gatttgttag ccagtgatat aaaacaaagt caagtagaca
     5581 ataacgttgg tggtgggatg attggagcaa tcctttatca ttttacttac ttcttgattg
     5641 cacagcctgg aagttattta gcagcagtgc tattactggc aggaggagca ttcctgttca
     5701 gtaatctgga aggctatcag ctgttaaatg gtatccaatc aattggagaa cgagtacagg
     5761 aattattgga aggtgatcct gccaagcaag ctcgaaagca agcggcaaag gaagagagaa
     5821 tgaaacagcg cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa
     5881 aagaagctgc agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg
     5941 atgaatggca ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca
     6001 gttttcaaga aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac
     6061 agaccaatac agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag
     6121 aatttgagat tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc
     6181 ttgattctat accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg
     6241 gcgtattaga acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct
     6301 taggaccatc cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa
     6361 ttgtgaactt aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag
     6421 caccgattcc aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg
     6481 tttcctttag agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac
     6541 ctttaggacg tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact
     6601 tactcgtggc tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa
     6661 gtatattaat gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg
     6721 ttgaattgaa tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta
     6781 gaaaagcagc gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat
     6841 tcgcggcaac cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact
     6901 tggaaaacgg aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag
     6961 ctgatttgat gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa
     7021 tggctcgggc agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta
     7081 tcacaggaat catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta
     7141 cagattctcg gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata
     7201 tgctattctt gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg
     7261 accacgaagt agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag
     7321 agaaaatgat gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg
     7381 agctgtttga agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc
     7441 tccaaagaag atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag
     7501 ctcatggagt tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg
     7561 aagaaacatc agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt
     7621 tgaaaaacga ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt
     7681 ttttccatct tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag
     7741 ttctattcga tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag
     7801 cgaaactttg agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg
     7861 aatgaagaat taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa
     7921 gtttgggaat ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg
     7981 aaacaattga tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc
     8041 gtccaagaag cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg
     8101 attgagactg tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta
     8161 ggatgtaata tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg
     8221 actgctggag aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat
     8281 cttggcgaac gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac
     8341 gacaatgtaa tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct
     8401 cggcacatta cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac
     8461 ggcttgcaag tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg
     8521 atcatgcgga tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat
     8581 ttagagaaaa caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat
     8641 agaccagagc atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat
     8701 gtcaacttga ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa
     8761 gctgatgtat tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt
     8821 aaagaacatg gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa
     8881 aaaacagttg ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt
     8941 tttcttaatt aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat
     9001 aaagcttttt ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac
     9061 agcctgcttg atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct
     9121 ttcttgtgta gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt
     9181 gatttcataa ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc
     9241 taaatcggtc gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt
     9301 cataaacaca tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag
     9361 tacaccataa ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt
     9421 accgtaatag aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac
     9481 caatccggta gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc
     9541 gtcggcacta tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt
     9601 tccgatttgg attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt
     9661 agattcgact tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc
     9721 ttcggcaata attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag
     9781 tccgtccatt acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt
     9841 cgaaccaata gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac
     9901 agtatcgcaa gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc
     9961 atgctggatc aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc
    10021 gacaacctct atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc
    10081 agtcttccct ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt
    10141 ttcatacgca tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat
    10201 ttccttcttt cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc
    10261 tgtagatgga gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca
    10321 ccttgaaatc atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca
    10381 ataatgaatt tttgttcctc ttagtatttc taccgattcc ctttgctttt tt
//
LOCUS       DAZUMB010000090        10335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000090 DAZUMB010000000
VERSION     DAZUMB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.092"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACG80L_002033"
     CDS             complement(25..162)
                     /locus_tag="ACG80L_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5024251.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG80L_002034"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG80L_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5024252.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACG80L_002035"
     CDS             complement(1599..1853)
                     /locus_tag="ACG80L_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024253.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACG80L_002036"
     CDS             complement(1878..3020)
                     /locus_tag="ACG80L_002036"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS5024254.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACG80L_002037"
     CDS             complement(3080..4432)
                     /locus_tag="ACG80L_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5024255.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACG80L_002038"
     CDS             complement(4503..4847)
                     /locus_tag="ACG80L_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5024256.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACG80L_002039"
     CDS             complement(4857..5231)
                     /locus_tag="ACG80L_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5024257.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACG80L_002040"
     CDS             complement(5244..5558)
                     /locus_tag="ACG80L_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5024258.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG80L_002041"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG80L_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ACG80L_002042"
     CDS             complement(8976..9638)
                     /locus_tag="ACG80L_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024259.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         2817 a         2318 c         1755 g         3445 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattc
//
LOCUS       DAZUMB010000091        10258 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000091 DAZUMB010000000
VERSION     DAZUMB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.093"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..1091)
                     /locus_tag="ACG80L_002043"
     CDS             complement(243..1091)
                     /locus_tag="ACG80L_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5024260.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1255..2157
                     /gene="gnd"
                     /locus_tag="ACG80L_002044"
     CDS             1255..2157
                     /gene="gnd"
                     /locus_tag="ACG80L_002044"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS5024261.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2159..3160
                     /locus_tag="ACG80L_002045"
     CDS             2159..3160
                     /locus_tag="ACG80L_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS5024262.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3157..3441
                     /locus_tag="ACG80L_002046"
     CDS             3157..3441
                     /locus_tag="ACG80L_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS5024263.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3474..4271
                     /locus_tag="ACG80L_002047"
     CDS             3474..4271
                     /locus_tag="ACG80L_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5024264.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4285..5118
                     /locus_tag="ACG80L_002048"
     CDS             4285..5118
                     /locus_tag="ACG80L_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5024265.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5136..5993
                     /locus_tag="ACG80L_002049"
     CDS             5136..5993
                     /locus_tag="ACG80L_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5024266.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6015..6524
                     /locus_tag="ACG80L_002050"
     CDS             6015..6524
                     /locus_tag="ACG80L_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5024267.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6561..6962
                     /locus_tag="ACG80L_002051"
     CDS             6561..6962
                     /locus_tag="ACG80L_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024268.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7074..7427
                     /locus_tag="ACG80L_002052"
     CDS             7074..7427
                     /locus_tag="ACG80L_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024269.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7461..7904
                     /locus_tag="ACG80L_002053"
     CDS             7461..7904
                     /locus_tag="ACG80L_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5024270.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7921..9006
                     /gene="uxuA"
                     /locus_tag="ACG80L_002054"
     CDS             7921..9006
                     /gene="uxuA"
                     /locus_tag="ACG80L_002054"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5024271.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8999..9712
                     /locus_tag="ACG80L_002055"
     CDS             8999..9712
                     /locus_tag="ACG80L_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5024272.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9919..10068)
                     /locus_tag="ACG80L_002056"
     CDS             complement(9919..10068)
                     /locus_tag="ACG80L_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024273.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3421 a         1629 c         2235 g         2973 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1141 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1201 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1261 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1321 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1381 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1441 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1501 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1561 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1621 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1681 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1741 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1801 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1861 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1921 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1981 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     2041 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2101 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2161 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2221 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2281 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2341 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2401 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2461 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2521 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2581 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2641 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2701 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2761 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2821 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2881 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2941 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     3001 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3061 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3121 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3181 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3241 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3301 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3361 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3421 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3481 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3541 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3601 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3661 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3721 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3781 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3841 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3901 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3961 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     4021 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4081 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4141 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4201 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4261 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4321 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4381 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4441 gcgatgattg ttacagccgc tttgcaaatg atttatatgg ggggtttttc tccaggagga
     4501 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4561 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4621 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4681 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4741 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4801 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4861 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4921 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4981 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     5041 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5101 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5161 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5221 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5281 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5341 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5401 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5461 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5521 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5581 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5641 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5701 tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5761 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5821 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5881 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5941 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     6001 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6061 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6121 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6181 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6241 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6301 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6361 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6421 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6481 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6541 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6601 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6661 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6721 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6781 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6841 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6901 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6961 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     7021 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7081 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7141 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7201 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7261 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7321 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7381 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7441 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7501 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7561 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7621 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7681 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7741 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7801 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7861 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7921 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7981 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     8041 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8101 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8161 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8221 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8281 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8341 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8401 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8461 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8521 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8581 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8641 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8701 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8761 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8821 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8881 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8941 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     9001 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9061 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9121 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9181 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9241 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9301 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9361 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9421 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9481 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9541 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9601 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9661 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9721 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9781 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9841 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9901 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9961 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
    10021 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10081 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10141 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10201 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttc
//
LOCUS       DAZUMB010000092        10193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000092 DAZUMB010000000
VERSION     DAZUMB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.094"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACG80L_002057"
     CDS             120..626
                     /locus_tag="ACG80L_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5024274.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACG80L_002058"
     CDS             643..1542
                     /locus_tag="ACG80L_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS5024275.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACG80L_002059"
     CDS             1629..2513
                     /locus_tag="ACG80L_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5024276.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="ACG80L_002060"
     tRNA            complement(2705..2776)
                     /locus_tag="ACG80L_002060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="ACG80L_002061"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="ACG80L_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS5024277.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="ACG80L_002062"
     CDS             4319..5410
                     /locus_tag="ACG80L_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS5024278.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="ACG80L_002063"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="ACG80L_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5024279.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /gene="pgk"
                     /locus_tag="ACG80L_002064"
     CDS             6562..7752
                     /gene="pgk"
                     /locus_tag="ACG80L_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS5024280.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /gene="tpiA"
                     /locus_tag="ACG80L_002065"
     CDS             7856..8611
                     /gene="tpiA"
                     /locus_tag="ACG80L_002065"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS5024281.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="ACG80L_002066"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="ACG80L_002066"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS5024282.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DAZUMB010000093        10085 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000093 DAZUMB010000000
VERSION     DAZUMB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.095"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..759)
                     /locus_tag="ACG80L_002067"
     CDS             complement(247..759)
                     /locus_tag="ACG80L_002067"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS5024283.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACG80L_002068"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACG80L_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS5024284.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ACG80L_002069"
     CDS             complement(3212..3655)
                     /locus_tag="ACG80L_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS5024285.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ACG80L_002070"
     CDS             complement(3656..4444)
                     /locus_tag="ACG80L_002070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5024286.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACG80L_002071"
     CDS             complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACG80L_002071"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS5024287.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ACG80L_002072"
     CDS             complement(5559..6146)
                     /locus_tag="ACG80L_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS5024288.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACG80L_002073"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACG80L_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS5024289.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ACG80L_002074"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ACG80L_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5024290.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ACG80L_002075"
     CDS             complement(9063..9998)
                     /locus_tag="ACG80L_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5024291.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2297 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataa
//
LOCUS       DAZUMB010000094        10074 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000094 DAZUMB010000000
VERSION     DAZUMB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.096"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..382
                     /locus_tag="ACG80L_002076"
     CDS             152..382
                     /locus_tag="ACG80L_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS5024292.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            479..1780
                     /gene="murA"
                     /locus_tag="ACG80L_002077"
     CDS             479..1780
                     /gene="murA"
                     /locus_tag="ACG80L_002077"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5024293.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1793..1969
                     /locus_tag="ACG80L_002078"
     CDS             1793..1969
                     /locus_tag="ACG80L_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5024294.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2104..2400
                     /gene="yidD"
                     /locus_tag="ACG80L_002079"
     CDS             2104..2400
                     /gene="yidD"
                     /locus_tag="ACG80L_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS5024295.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2464..2931
                     /locus_tag="ACG80L_002080"
     CDS             2464..2931
                     /locus_tag="ACG80L_002080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5024296.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3016..3900
                     /locus_tag="ACG80L_002081"
     CDS             3016..3900
                     /locus_tag="ACG80L_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5024297.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4014..4250
                     /locus_tag="ACG80L_002082"
     CDS             4014..4250
                     /locus_tag="ACG80L_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS5024298.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4338..4418
                     /locus_tag="ACG80L_002083"
     tRNA            4338..4418
                     /locus_tag="ACG80L_002083"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4372..4374,aa:Tyr,seq:gta)
     gene            4425..4496
                     /locus_tag="ACG80L_002084"
     tRNA            4425..4496
                     /locus_tag="ACG80L_002084"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4457..4459,aa:Gln,seq:ttg)
     gene            4621..4791
                     /locus_tag="ACG80L_002085"
     CDS             4621..4791
                     /locus_tag="ACG80L_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024299.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5011..5718
                     /locus_tag="ACG80L_002086"
     CDS             5011..5718
                     /locus_tag="ACG80L_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5024300.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5778..6422)
                     /locus_tag="ACG80L_002087"
     CDS             complement(5778..6422)
                     /locus_tag="ACG80L_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS5024301.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6549..6836
                     /locus_tag="ACG80L_002088"
     CDS             6549..6836
                     /locus_tag="ACG80L_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024302.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6944..7264
                     /locus_tag="ACG80L_002089"
     CDS             6944..7264
                     /locus_tag="ACG80L_002089"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5024303.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7345..8658
                     /locus_tag="ACG80L_002090"
     CDS             7345..8658
                     /locus_tag="ACG80L_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5024304.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8655..9374
                     /locus_tag="ACG80L_002091"
     CDS             8655..9374
                     /locus_tag="ACG80L_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS5024305.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9502..10053
                     /gene="raiA"
                     /locus_tag="ACG80L_002092"
     CDS             9502..10053
                     /gene="raiA"
                     /locus_tag="ACG80L_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS5024306.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3358 a         1666 c         2150 g         2900 t
ORIGIN      
        1 gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata
       61 tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta
      121 ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct
      181 tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg
      241 cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt
      301 ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg ctttatgccg
      361 aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt
      421 ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat
      481 ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc
      541 taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac
      601 attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt
      661 aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg cttcaagaca
      721 attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat
      781 gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat
      841 tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat
      901 tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt
      961 agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg
     1021 aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt
     1081 gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt
     1141 ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt
     1201 tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca
     1261 taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg
     1321 cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca
     1381 tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg
     1441 tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg
     1501 ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga
     1561 attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc
     1621 tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata
     1681 ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg tagacatgga
     1741 tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa
     1801 ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct
     1861 tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa
     1921 agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat
     1981 cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa aagaaaacag
     2041 aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa
     2101 ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca
     2161 tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa
     2221 acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct
     2281 tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg aaataagaaa
     2341 gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac tgagcgataa
     2401 agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata tgggggacaa
     2461 aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat
     2521 cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa
     2581 aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc
     2641 aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca
     2701 gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat acgaaccggc
     2761 agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga gattggtcga
     2821 gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta
     2881 agggatcaag taattttagc aattcactta cctaaaaata gctttaacta ataaactttc
     2941 aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa
     3001 aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat
     3061 ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg
     3121 gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc
     3181 gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga
     3241 atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt
     3301 caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa
     3361 ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg
     3421 gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg agaatttggg
     3481 ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt acagatggct
     3541 tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa agatgtcttt
     3601 gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat
     3661 cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc
     3721 ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg
     3781 acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga atacagaaac
     3841 gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg ggagaattaa
     3901 gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata ggagattttt
     3961 acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt
     4021 caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta
     4081 agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct
     4141 atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc
     4201 ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca
     4261 aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag
     4321 gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg
     4381 ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc
     4441 ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata
     4501 ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct accttgttcc
     4561 ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg agggatccac
     4621 atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca
     4681 gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa
     4741 tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa
     4801 aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa
     4861 catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg tctaaatcct
     4921 ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata
     4981 atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag
     5041 attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt
     5101 ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca
     5161 attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg tacatatgtt
     5221 gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa
     5281 tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca
     5341 agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt
     5401 cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca aaagctgatc
     5461 gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc
     5521 gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt
     5581 gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca gatttcttat
     5641 tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag
     5701 ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa ataaaagaga
     5761 atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata agaaacagga
     5821 ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtca
     5881 gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt
     5941 ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc
     6001 atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga
     6061 gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa
     6121 tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc
     6181 ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt
     6241 tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct
     6301 tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt
     6361 gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc
     6421 atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt
     6481 caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa aggtaggtgg
     6541 atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca
     6601 atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta
     6661 acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc
     6721 ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg
     6781 acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa
     6841 accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt aacagacgcg
     6901 aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta aaaaaacaat
     6961 catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc
     7021 tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg acgcagataa
     7081 caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc gctttatgaa
     7141 agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca tcaatatggc
     7201 tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga
     7261 ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc
     7321 gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga
     7381 gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca
     7441 gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt
     7501 gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt agggcgatgc
     7561 gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc
     7621 tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc
     7681 cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg
     7741 ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg
     7801 cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa
     7861 atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt
     7921 acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc
     7981 gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa
     8041 ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact aaaggaaatc
     8101 aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt acttcctgaa
     8161 cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa gaagataaaa
     8221 tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt attttgtcca
     8281 agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc gagatatgag
     8341 atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa aatgcgagaa
     8401 gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac atttgaaaga
     8461 atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc tttggtacaa
     8521 atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata ctttttctat
     8581 gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa
     8641 gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa aatttaacat
     8701 tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca gtttgtcgcg
     8761 cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca atccatttca
     8821 atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt gactgtcaag
     8881 aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt gtgtacgatg
     8941 aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga ctagcaggca
     9001 cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc tattgtccaa
     9061 tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc gagatgttga
     9121 aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg ctggctcaag
     9181 caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt gcagtcacct
     9241 tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca acaggacaga
     9301 ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc cgaaccattt
     9361 ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca aataacctag
     9421 agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg
     9481 tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta
     9541 actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt
     9601 gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa
     9661 gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat
     9721 ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa
     9781 acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc tactgcttta
     9841 ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc
     9901 ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat gctgggacat
     9961 aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa
    10021 gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata acaa
//
LOCUS       DAZUMB010000095        10008 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000095 DAZUMB010000000
VERSION     DAZUMB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.097"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            266..535
                     /gene="rpsN"
                     /locus_tag="ACG80L_002093"
     CDS             266..535
                     /gene="rpsN"
                     /locus_tag="ACG80L_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5024307.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            550..729
                     /gene="rpmF"
                     /locus_tag="ACG80L_002094"
     CDS             550..729
                     /gene="rpmF"
                     /locus_tag="ACG80L_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5024308.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            761..1138
                     /locus_tag="ACG80L_002095"
     CDS             761..1138
                     /locus_tag="ACG80L_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024309.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1159..1308
                     /gene="rpmG"
                     /locus_tag="ACG80L_002096"
     CDS             1159..1308
                     /gene="rpmG"
                     /locus_tag="ACG80L_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5024310.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1332..2315
                     /locus_tag="ACG80L_002097"
     CDS             1332..2315
                     /locus_tag="ACG80L_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5024311.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2345..2467
                     /locus_tag="ACG80L_002098"
     CDS             2345..2467
                     /locus_tag="ACG80L_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5024312.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2485..4008
                     /locus_tag="ACG80L_002099"
     CDS             2485..4008
                     /locus_tag="ACG80L_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5024313.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4032..4271
                     /locus_tag="ACG80L_002100"
     CDS             4032..4271
                     /locus_tag="ACG80L_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024314.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4464..4925)
                     /locus_tag="ACG80L_002101"
     CDS             complement(4464..4925)
                     /locus_tag="ACG80L_002101"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5024315.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5120..5338)
                     /locus_tag="ACG80L_002102"
     CDS             complement(5120..5338)
                     /locus_tag="ACG80L_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024316.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5456..7072
                     /locus_tag="ACG80L_002103"
     CDS             5456..7072
                     /locus_tag="ACG80L_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5024317.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7313..8653)
                     /locus_tag="ACG80L_002104"
                     /pseudo
     CDS             complement(7313..8653)
                     /locus_tag="ACG80L_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9091..9237)
                     /locus_tag="ACG80L_002105"
     CDS             complement(9091..9237)
                     /locus_tag="ACG80L_002105"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024318.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9276..9470)
                     /locus_tag="ACG80L_002106"
     CDS             complement(9276..9470)
                     /locus_tag="ACG80L_002106"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5024319.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9380..9712)
                     /locus_tag="ACG80L_002107"
     CDS             complement(9380..9712)
                     /locus_tag="ACG80L_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5024320.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3315 a         1597 c         1983 g         3113 t
ORIGIN      
        1 gctacttaaa acggtatttg tgtttgattg gactaataaa agtgaattct tatttttcag
       61 agcctaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga taaattcgtt
      121 ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt gacaagagca
      181 actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat
      241 caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa
      301 acaaatggcg atgattgaaa agtatgcaga taaacgtcag gaattaaaag ccgcaggaga
      361 tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga
      421 tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc gtattaagtt
      481 tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga
      541 ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg gttaagaaga
      601 actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg tgattataga
      661 cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat taaaggagct
      721 tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta tttacccacc
      781 tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc tagataaatg
      841 cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt taccgaccca
      901 acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct tatcatttga
      961 tgatttttta agaatctatc caatttttga agagtatgac gaggagttat ttcaatgtat
     1021 cgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac aattggaatc
     1081 tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag atcgataagt
     1141 attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga aactggagaa
     1201 cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga attaaaaaag
     1261 tattcgccaa aattgcgcag acgggcaatt tttaaagaag tgaaatagct aagtggtaaa
     1321 aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa
     1381 caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa attattataa
     1441 ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat agcgaggaac
     1501 gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa
     1561 tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca caaatcatca
     1621 ttgaaactac aggtatagca gatccccaac caatcattca aacactttta acagcgcctc
     1681 aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt gacagttttg
     1741 aacttattga aagagaacca caagccctta agcaacttgt catggcagat cgcatttttg
     1801 tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga gaaatcaaaa
     1861 gtattaaccc tttatccgat attcgaatgt tcacacatag tgaaacgatt caaaaaaagg
     1921 atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa
     1981 atcactataa tggcaaatac tatcatcaac accatcaagt acatgagttt aaatcaattc
     2041 tcttaacttc agatcaagac ataaaaaaag attttttact ccaatggatt gactggctga
     2101 ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta caagagcagg
     2161 attttatagt tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac accactcgac
     2221 aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac
     2281 agagttttga taaattactg gcggaaagca aataatgata agtagtgatt gggggtaatt
     2341 tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc cgaatattaa
     2401 aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa tgaagaaatt
     2461 agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat tgggatagca
     2521 gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc gaatgataaa
     2581 gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac caaaaacatc
     2641 gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc tcatgactat
     2701 gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt atatcataac
     2761 gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc tgggcctaat
     2821 gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga agatcatgat
     2881 cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc acccaaaatg
     2941 gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc gaaaaaagca
     3001 aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac
     3061 tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt
     3121 ggatatttag ctttggatta tggattgaaa caagttccta ttgctggact aagtcctgaa
     3181 gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa aaataagatc
     3241 aattatattt actttgaaaa gaatgctaat gataaaattg caaaaacttt ggccaacgaa
     3301 gctgggataa aattggaagt actgaatcct cttgaaagtc ttacaaaaga acaaatggat
     3361 aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga aaaaacaact
     3421 atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa gacagttgct
     3481 aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa
     3541 tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt cgattacaaa
     3601 gcaaaaatca ataaagacat gacagctgcc gagtacaaag attattatac tactggctat
     3661 aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt agtcaacggt
     3721 gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga
     3781 aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg ttttaaatat
     3841 attcaattta gtgatcatgg aattgcacca agtaaggcag agcatttcca tattttcttt
     3901 ggtggagaaa gtcaagaaaa actctataat gaattgcata attggcctac tttctatcca
     3961 gcttcattgt ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt
     4021 ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat attgatgctt
     4081 atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta gctgatattg
     4141 ctgcaacttt acatgttagt attgacacag ttcatagagt attaaaacgg gatcctgatt
     4201 gttgttttat caaggtcaat actggaagaa gtagacgttt agcaaaacgg cataatagct
     4261 tggccaatta attgataata ccagagtaga tcatttttcc cttatttgga tttgatctgc
     4321 tttttattat gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga agacttcata
     4381 aggtgttctc caatctaaac actttctagg tctgttattc agtttttcaa tcatctgatt
     4441 tatcgttgag ttatctaccg tagctatctc tttcgatttt ggcaagtatt ctcgaatgag
     4501 tctattcgta ttttaatttg ttcattgttg ccaagggcat gaggatctgg aaaataaaag
     4561 ggcatattat tcaatgcttc tgtaactgcg aaatgattgg agaattcttt tcctctatct
     4621 ggagtgatcg ttttgaccgc ctcatttgga atactgctta aaagttcaat cattttctcg
     4681 ctcacataag cggctttctt tctggggacc ttctttcctg ctaaaaggaa gcatgatttt
     4741 ctgtctgtca aagtcactaa acaagtacca ccagttttgc cagctacaat atcggctttc
     4801 caatgaccaa aatcacttct atcatctgcg gattttggac ggccatggat cgaatgagta
     4861 aggcaaatct ttccacgaga ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg
     4921 tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg gttctgtatc gaaaagatca
     4981 ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc
     5041 aaacgattcg agatttcctc tggcgaccac tgaaaatcga caaataaatg acgaacaatt
     5101 gaccaaactc acggatcatt tagaatgtga taacgtccgc atttttttct tcgataagca
     5161 taattttttt ccgctttgga aggtgaatag tttttttctt gtttcttctc agttcatgta
     5221 aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat actaatagaa aatccttctt
     5281 cgtgaagcag aaagattctt tctcgttcga gtatagtaag atgacgatac tggctcataa
     5341 ttattttcct tctaggagtg ttctcgacaa acttatacta gttggatatc gattatgact
     5401 catttttatg tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa
     5461 aaaaatacct attacaatct taactggcta tcttggttca ggaaaaacga tacttctaaa
     5521 tcatattctg tacggtgatc acggaaagga aattgctgtc attgtaaatg acatagggga
     5581 agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact
     5641 tgtttcgatg ccgaatggat gcatttgttg tacattacgc gaagatttga ttgttgaact
     5701 agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt caggaatcag
     5761 caaaccaatt ccaattattc aagcaattat attagctaaa aaccctttag acattgattt
     5821 gacccaacga ttagagattg gtgccattat tatagtagtg gatgtaaaac gtttttggga
     5881 taatttcgaa acgggtgaaa agattcagga aagaaaagct gaaggtaatg tagttgaaga
     5941 agaacaggat attcgtggct tgttaattga tcaattcgag tgttgtaaca tattactttt
     6001 aaataatagt gacttagtgt caaccaagac gatttacaaa attaacggtc taattgctaa
     6061 gttacagtcc aatgccaaaa taattaggac aattaaaggt gaagtagact tatcgaaaat
     6121 tctaaataca aatcttttta atatagatga gacttcctat tcagtgggct ggttcaaaga
     6181 gttagagctt ggctatcaaa atcatattcc tgaaacagaa gaatatggta tctcaagttt
     6241 tatctatcga aatcggttac tccttgacgc acaaaaattt gcccatctta cggataaaaa
     6301 ttttcctggc aatattacaa gagcaaaagg gtttctttgg tttgcccaac atagtgattt
     6361 ttcgacgatc tttgaaacat cgggtagcta taaaagtatt gaatcattaa attattgggt
     6421 agctagttta cctaaaattg agtgtcaaat gattatacaa aaaaatcccg aattgaagga
     6481 agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg gtatgaagat
     6541 ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc ttaatgcaga
     6601 taatttccga aaaatttcac catttccatg gaaaaagtct aatatgttta gccagatatc
     6661 gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa ttattggcat
     6721 tttgattatt acctctttta ttcccttatt tgtattctct atagaaaagg agaatgaaac
     6781 aactgaagat gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt
     6841 cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg gacaatataa
     6901 tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc ctgatcaaat
     6961 cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt taccccataa
     7021 gttagttatt agtattgaga aaaaagaaag cacagattac tacatttact aatctggaaa
     7081 tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca ggggctttat
     7141 taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc acaagtatct
     7201 ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt tggctcttcg
     7261 tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag
     7321 ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagta ttcttagttt
     7381 tcttaaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctat
     7441 agccataggc aattcgtttt agtactttga tatggttatt caaacattct atagggccgt
     7501 tggaataggg aacgcgcaac gctcgtttga tttctgtttg gtatttcttg aaggtcgtga
     7561 acactggttg gtagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt
     7621 caggaacatc ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg tagcctcgct
     7681 ttaaggaatc atcataagat aatagccgat cgactatctc tgcttctgtc agatgatctc
     7741 gaaatgacgg acgccagtag cgatgctgac ctttcaattc ccacgcattc ttttgaagta
     7801 gtttccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta
     7861 cttgaattcg gtggttggta aaagcccgac caatgtgttg gctaatatga aagcgatcgg
     7921 cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat
     7981 ctgaaaccac tagacgcact tgttcacgga cttttatagg gtagcgcaag aaatagtcgc
     8041 gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca
     8101 tcatgacaaa gctcatggct cccgagacat ttttcactga tttgaattca tcgaaacaca
     8161 ccacttctgg taacagctgg ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc
     8221 gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat caaagacata gaaagaggtt
     8281 cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc aatggagcag cgacgatcac
     8341 taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt
     8401 taatccgcag aatggtcttg tagttactga catcattcaa taagatggat acttttttcc
     8461 agccctattt aatgatcgtt tgttcatttt gatagtggcg atgaaagcat tcctccggtt
     8521 gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attgattcgc tctttgcgaa
     8581 aggcatcttc atgaaagata agatttaggt ctagaatatc tagtaactct ttggtaaact
     8641 ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta ttctaaagga
     8701 ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt
     8761 caccaacact agatattata gatcccttat tttcttcgct tcaaattgtc aaggagtcat
     8821 cataaatttt gtatggattc ttttgtttgg gtagtagtct tctaaataat cttggattaa
     8881 caccttcatc tctattttac ttttatattt gttggagtcg attaattctc atttgattga
     8941 tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc ccagtttggc tcactgaatg
     9001 tgtgatgtgg tggtcgttca acagctcttg ataaataaaa gaccgatgct ggctacctct
     9061 atcggaatga attaccgtag gcaattctcc ttaataaatt tgtaatggac ggacgacaat
     9121 ctcttttttc atgtgtgaat ctaccatata gtaacgtaga atacgagttg ctaagtcaat
     9181 ataggtggac aaatagaggc gaccgtcatt acatggaatg taagtaatat ccatcaccca
     9241 aacagcatca atttggttct gttcaaatta tcgattcaac atttttttac catttctttg
     9301 attgtttttt ctctaccatg agaagatttg cgtcgatgaa atcctcttgt gcataaattc
     9361 atttcacgca taagaacagc tacaacacgt ttattgattt cgattccttc atcgtaaagt
     9421 ttttgagtga tccctggaga tccgtatcga tctttgtgtt cgtagaacac tcgtcttcct
     9481 cttttagtcg tttattttcc tgttcaagaa tgtagaaatc tgctttatca ggtaatcttt
     9541 ttccttgtcc agcaaaagca tttttcccgt atcgacgata ttctgaaacc catcgggaaa
     9601 ttgttggacg agagatattg tattcttcag caattttctt ggtagttact tttttctcaa
     9661 ggatatcctg tgaaatcttt agtttaaatt tacgatcata tgtttttctc aatagagttc
     9721 attccttccg gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca acttagtatc
     9781 agggtttgtc aactaaactg tggaagttga tagataagag tttttaacaa ccaaaattct
     9841 cttgactatt tttgaaatca gacaaatttt gattggacac atagttgaat aaatctaccg
     9901 tcgttacgga aggtaaatcg catgcttttc aattctagag gagaagtcct cgttgaagta
     9961 ccatagccca gttctggatt cgacctcctg cccacttcat acgtaaat
//
LOCUS       DAZUMB010000096         9983 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000096 DAZUMB010000000
VERSION     DAZUMB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.098"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..3393
                     /gene="fss3"
                     /locus_tag="ACG80L_002108"
     CDS             166..3393
                     /gene="fss3"
                     /locus_tag="ACG80L_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS5024321.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3506..3820
                     /locus_tag="ACG80L_002109"
     CDS             3506..3820
                     /locus_tag="ACG80L_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5024322.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3833..4207
                     /locus_tag="ACG80L_002110"
     CDS             3833..4207
                     /locus_tag="ACG80L_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5024323.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4217..4552
                     /locus_tag="ACG80L_002111"
     CDS             4217..4552
                     /locus_tag="ACG80L_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS5024324.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4635..5975
                     /locus_tag="ACG80L_002112"
     CDS             4635..5975
                     /locus_tag="ACG80L_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5024325.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6053..6727
                     /locus_tag="ACG80L_002113"
     CDS             6053..6727
                     /locus_tag="ACG80L_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5024326.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6720..6884
                     /locus_tag="ACG80L_002114"
     CDS             6720..6884
                     /locus_tag="ACG80L_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024327.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6960..8102
                     /gene="dcm"
                     /locus_tag="ACG80L_002115"
                     /pseudo
     CDS             6960..8102
                     /gene="dcm"
                     /locus_tag="ACG80L_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8092..8382
                     /locus_tag="ACG80L_002116"
     CDS             8092..8382
                     /locus_tag="ACG80L_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024328.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8641..9825
                     /gene="mobT"
                     /locus_tag="ACG80L_002117"
     CDS             8641..9825
                     /gene="mobT"
                     /locus_tag="ACG80L_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5024329.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9822..9959
                     /locus_tag="ACG80L_002118"
     CDS             9822..9959
                     /locus_tag="ACG80L_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5024330.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3305 a         1682 c         2252 g         2744 t
ORIGIN      
        1 ctaatttaaa ggcagtatat ttcgtatttt agattaaatt tttaggctca ttcattctca
       61 aatccattct attcagtcaa taaaaatttt aaaaagactt tataaacaaa gatgttcaag
      121 cgagcatctt atttttttgt gcttaatttg aaaggagaac atttcatgta taaaaaacaa
      181 aaaaagaaat tatgtgtatt aactattttg agtttggctg ttcaagcaat tgttggagca
      241 attgctccta cgattacttt tgctgatgaa attacccatc cacaaacggt aaccgtagaa
      301 ctagatttag cacatcaata tgctgttgaa ggaactttta gtgatggtcg tccaatgtca
      361 gaagtcactg ttccgcatta tgcagtgtac aacggcttta aacaagatat tttctgtatt
      421 gaaccaggag tcccgattta caatgagttc actcctggct atgagaaaaa cccgttacca
      481 gatatgtccg ataaagccaa attagtttcc gttttatgga aaaaagtagg tacagatgca
      541 gatactcata ttgtggcaca gaagatgatc tggcaagaag ttaatgggta tacccttcac
      601 tcaataaaac gatcagatgg tagtgccgta aatatcgcag ccattgaggc aaaaataaat
      661 caagccattg acgattatca gaagaaacca accttccata atagtacggc taaaacagta
      721 ttagggcaat cgactactgt gacggatacg aataatctga atttgtcaga gttcgatgag
      781 gttgtggaaa atgccgcaaa cattgattat cgtgtgaatg gtaatcagtt gataatcacc
      841 ccaaatgcca attcaaaaga aagtggtgtg ctgacgctta agaaatctgc tggtaccgga
      901 acacctgtcg cctataaaaa ggctggccaa cagactttaa tggccggggc gattgataaa
      961 cctaatacct acacaatcaa aattgatgtg gaaacggagg gttctttaaa gatcaaaaaa
     1021 gtcgataaag aatcgggtga tgttgtacca ggaacggttt tccatttaga ctttggaaaa
     1081 actttacctg caaaagacgt gactactgac aaagaaggca ttgctacatt ggatgagatt
     1141 cctcatggta caaaagtaac cattaccgaa aaatcagtac cggcgcctta tacgattgat
     1201 accaccccca tgactaccac cattaaagca ggcgaaacca tttacgtgac ttcaaaaaac
     1261 gcacgggaaa aagggcaaat cattctggat aaaagcggag ttgaaacagg gagtgatctt
     1321 tggaatgaca attattcctt agctggtaat acgtttgcca ttcgcaaaga cagtccaacc
     1381 ggtgaaatcg ttcaagaaat gaccacggat gaaaacggtc atgcggaaac accaaaagag
     1441 attgctaatg cgttggaatt gggaacctat tatgtgaccg aaactaaagc tagtcatggg
     1501 ttcgtgaata cgttcaaacc tgtaaaagtc gaattgaagt atgccaatca aaccgtagcc
     1561 cctgttacca gtaacgtaaa agggcaaaat caagaagtca ctggcgaaac cactttgacg
     1621 aaagaagaca aagatactgg ggataaagca caaggtaagg cagtctttga gggtgctgaa
     1681 tacaccttgt ttactgctaa ggatggaaaa gctgttaaat ggagtgaggc ttttaaacca
     1741 gagttagcga agggaacgaa aggttctgat gaaacggtca ctttgacttt agacgaaaag
     1801 aaccaagttg ccgttaaaca tttagctatc aacgagtatt attggcaaga aacaaaagcg
     1861 cccgaaggat atagccttga tgaaacaaag tatcctgtat ccatcaaaaa ggtggatgat
     1921 agcgaaaaaa atgccgtgat tacccgaaat gttacggcaa aagaacaagt gattcgcttt
     1981 ggctttgatt tctttaaatt tgctggatca gctgcgggca ctaccgaaac tggcttcaat
     2041 gatctgacct ttaaagtgtc gccattggaa gggaccaatg aaatcacagg tgccgaagat
     2101 gaagcgacca cagcttataa cgagcaatta gggtttgatg gttatggcaa gtttgaaaat
     2161 cttccttatg gggattattt acttgaagaa gtagaagccc cagaaggttt tcaaaagatt
     2221 acgccattag aaatccgttc tacttttaag gaaaacaaag aagactttgt gaagagtgag
     2281 tatgtcttta ccattaccga acaagaccaa aaacaaccaa ttaaaacggt gaccgttcct
     2341 tacgagaagc tgacgaacaa agcattttct gttagtttga accgtttaat gctctatgat
     2401 ttgcctgagg aagaagatag tttaacttct cttgcgactt ggaaagacgg aaacaaagaa
     2461 ttaacttctc tggattctac cgagctagtt gataaattga gttacaatct acatgaaatc
     2521 aaagaagact ggtatgtggt agcgcaagcc attgatgtgg atgcgacaaa agctgcccaa
     2581 gaaaaagatg aaaaagccaa accagtggtg attgcagaaa caagcgcaac gttggcgaac
     2641 aaagagaaaa caggaacttg gaaaattcag cataaattaa ccgccgaaca agttttaaat
     2701 aaaactattg tcttattcaa ctatgtgtat gaaaataagg aagcttttga agcaggcgat
     2761 aagccagtag cgaaggatgt tagcttgaat aaccaagccc aaactgtcag ttgtacagtg
     2821 gaacaccatg tttctatcca aacaaaggcc cacctagaaa acggttctca gacctttact
     2881 catggtgatg tggtggatat gtttgatgat gtgtcgatta ctcatgatgt actggatggc
     2941 tcaaaagaag ctttcgaaac aattctgtat gctttattac cggatggtac aaacaaagaa
     3001 atttggaaat ctggcaagat tgattatgaa gtaaatgata aagaattcac caaaactgtg
     3061 cttgcgaaaa aagtagatac cggaaaatat ccagaaggaa ccaagtttac ttttgcggaa
     3121 atcaattacg ataaagatgg gaatatcaat ggaaaacaca atgaagattt gaaagaaaaa
     3181 tctcaaacct taacaccaaa agaagttcca accatactaa gtacgccgaa acaaccggaa
     3241 acaccaactg ttccaagtgg ctctcaagaa tctagtccca cagtgaagac cttccctcaa
     3301 accggggaga aaaattccaa tgttttactg ttcattggtt ttactttgat ttttgcgacg
     3361 gcgggctatt atttctggaa tcgccggaac taaggtgatg agatgataca agtaaatcct
     3421 aagaaccaac gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt gtatccaaaa
     3481 ctaacaatga aaagaggaaa agaagatgga attgaaattt gtcgtgccag atatggctga
     3541 aacctttgga aagttaagtt atgccggtga aggagaaatt ataacagaag gatatggacg
     3601 gaatactacg gtgattggtc gcagttatca tttgtattcc agtaagcaac gggccgatga
     3661 tattgaggtg gtagtagctg cggaagccgg tgaaaaggat tttgatcaag accaaccgct
     3721 aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc gtgggtttac
     3781 tgattacgtg ttgtatgttg atgatttagt gaaagcatag gaggaaaaga agatgagatt
     3841 aacagaaggt attgtcgtag attcaggttt aacatttggg aagttgcgtt tttccgcatt
     3901 acgccgagaa gtacggaagc aaaatgagga tggaacaatt ggtaatgagg taaaagaacg
     3961 aacctataat ttaaaatcct ctgcacaagg gcgaatgatt caagttagta tcccagctaa
     4021 tgtaccctta cgcgagtttg cttatgatgc agaggtagag ttggtcaatc caatcgtgga
     4081 taccgttgcc aattatgttt ttcgagaagg aactaccgtt aattggttca ttaaggcgga
     4141 tgatttagtc ttgaagcggc agccgaatca aggaaatcct acgaaccaaa atgaaagaaa
     4201 gaaataggtg aagattatgg aaattaatgt tgaacatatt ttagattgtc tcgatcaata
     4261 tggaaaaggc gagctgactg aggaacaact cgttaaagca ttaacctctg atgaaaaaat
     4321 gtttctgatt atacatcaag gtttacttgg agaaaatagc gacagtgaag aagactctga
     4381 tgtactagac tggttaggag aagaagagtc tttctttata gttgtcgaag taaatgaaaa
     4441 cttatgtcgg caggcagaat ccattttgga agaaattggt gtggagatgc cggatgctat
     4501 tgaaggcttt ttaaatcaat tggttgaaac gaaacaactc ccctcctcgt agcagtaaat
     4561 gactagcata actttttgaa attgcctaat gaaatcctat tgggcttttt cgattggagg
     4621 tggaaaccat caaaatgtac aaaggacatc gcatacgcgc aggagatcag cacttggttt
     4681 atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt
     4741 ttcaagagtt tagacaattt gatatttcta agctatcact ttctactatt gaaatcgttt
     4801 ggtctataaa agatttagta tgtctgcttg gaagtctcgc tttttcagga gctatgatat
     4861 tgctttatat tcactttttt cttgatcatt ggcgaagctt gtggcatcga caaaagctag
     4921 ctcggatgat tctagagaac cattggtatg aagtgaaaca aactcaaagt gaaggctttt
     4981 tcaaagattt gaatagtagc cgaaccagag agactatcag ttacttcccc aaaatctgtt
     5041 atcgaatgaa agatggatta ctgtctattc gcgttcaaat ttcacttggg aaataccaag
     5101 atcagctttt aaaattggaa aagaagttag aaagcggact gtattgtgag ttagtagaaa
     5161 aggaactcaa agactcctat gtggagtata ctttactgta tgatatgatc gccaatcgaa
     5221 ttgggataga cgaagtagtg gcagaaaatg ggactctgcg attgatgaaa aatcaagtat
     5281 gggcttatga ttctttaccc catatgttaa tcgctggtgg cacgggtgga gggaagacct
     5341 atttcctcct caccattatc gaagcactat tgaagtcaga tgcagaattg tttatcctcg
     5401 atcccaaaaa tgcagattta gctgatttag gtacggtgat gcctcatgtt tattcacaaa
     5461 aggaagaaat ttctgcttgt gtggaagatt tctatgaacg tatgatggct cgtagcaagg
     5521 caatgaaaga aatgtctaac tacaaaacag gagagaatta tgcctatctt ggacttccac
     5581 ccaacttttt aatctttgat gaatacgtgg cttatatgga gatgttaacg acgaaagaaa
     5641 gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt gggtcgtcaa tctggtttct
     5701 ttctgatttt ggcttgccaa aggccggatg cgaaatattt aggagacgga atccgcgatc
     5761 aattcaactt tcgcgtggct ttgggtcgta tgagtgaact cggatattcc atgatgtttg
     5821 gggaggtcga aaaaaacttc tttatgaagc acatcaaagg tcgaggttat gtggatacag
     5881 gtggcagtgt gattagtgag ttttatacgc cacttgtacc aaaagggtat gatttcttag
     5941 attcaattag taaattagaa aagatgaatg attgagtgat cttcaatact gactttatac
     6001 ttcaagtaac gtataatact tttatatagt aggaagaaag gggataatta atatggaaag
     6061 taatgattct tatttagagt atgcaaaagc aaataaggat aagtttattt cttatattat
     6121 caaagggaaa tcccccgaag ttggacgaga cgcagttttt atggcaggga gtcctggggc
     6181 tggaaaatca gaggttgcca ttggattagc tgagaattat gataaccatg tgattattga
     6241 tgcggattac tttcgcaccc agtttcctga ttataatgga aaaaactcta gtctctttca
     6301 aaaagcatct tcttggttgg tagagcaggc atttagatat gtcttagatc atggctattc
     6361 ttttattctg gatgcaactt ttgcaatatt aagtgctgaa aaaaatatct cgcgggcgtt
     6421 gaaaaaacat tttcgagtga cgattttcta tgtttatcaa gaccctaaaa tcgcttggaa
     6481 atttacacaa gaacgtgaaa ttgcagaggg acgtcatgtg ccgaaaaagg catttattaa
     6541 cgcttttttc aaatcaagag agaacattgc aaaagtaaag agtaaacatc cagatgtatt
     6601 gcttcatatc ataataaaag attatacgaa taatatttcc gaagttcatt atgctacgga
     6661 taatctagaa ttatttttac cactagggta tagtaggaaa gatttggagg ttcaattaca
     6721 tgactgagaa agaacaagta acgaaaattg taaagaaata caataagagc attgcggatt
     6781 tatcggaaaa tgctactgca aaagagttca aaacggtgat taaatatgta gccgatcagg
     6841 ccaatgaaaa acaacggaaa ttagttggat taaataaaaa atagtaggac aaattaataa
     6901 tgtatgagcc aagagcaaaa tgttcttggc ttttttcttt gcaatgaatg aggtaatgta
     6961 tgagattttt agatttattc gccggtattg gtggctttcg tttaggtatg gaacaggcga
     7021 gccatcattg tatcggtttt tgtgaaattg ataaatttgc tagacgtagt tataaagcaa
     7081 ttcatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca gatgagttta
     7141 ttcaatccct cggaccagtg gacatccttt gcggcggatt tccgtgccaa gctttttcaa
     7201 ttgcgggaaa gcggcaaaga ttttccgata ctcgaggtac tttattcttt gaaatcgctc
     7261 ggttcaccac tcttctccga cctaagcttt tattccttga gaacgtccga ggattgctca
     7321 atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga ttggggtatg
     7381 acgtggagtg gtaggtgctt aactcaaagg cctacattcc ccaaagccgg gagcggatat
     7441 tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc attggaccgt
     7501 cttcaacatc tgatcaaaac atcagaaatt tggtaaatat caatccttcc aatcgaggaa
     7561 tgggtggaca agtctatggt tcagatggcg tagcgccaac cttaacaggt gatgaaggaa
     7621 tcaaaatcgc actgccagca agggatggaa tctctgtagc aggactatta ccgggaaagt
     7681 ttgaacaagg taatcgagtt tatgaagtta ctggaaccgc ccccaccttg tcaacgaaac
     7741 aaggtggtac aaaaatcatg attcgaaaaa atgaaacaga atatcaagag gtgaagccag
     7801 aagacagtgt gaatctggct tttcctaatt ccacaagtcg ccgaggtcgt ctgggaaaac
     7861 agagtgttca taccttacta acaggagacc aacaagcgat cgtcacggat cagtatcaaa
     7921 ttcggaagct aacacctaga gagtgttggc gacttcaggg ctttcccgat tgggcttttg
     7981 atcgagattc tcaagtcaat tcagatagtc agttgtataa gcaagccgga aactctgtaa
     8041 cagttccggt catttttgat attgccagaa gattaaaaga gttggatgta catggtttat
     8101 gatgagttga ttggagaaat ttattgggtg attggaaaga ttcagtcaga cccagaatta
     8161 gaagaacaat tgcgacggtt aaaatttgat attcgcaaga atggtgtcaa agtcgctggc
     8221 gatccatatt taatgaatga ggagacggat gctcgaattg aaataaatca agtaattgca
     8281 gaatttgagc ggattgcgga ccaagcaaaa gaaccagata ttcgtcaata cttgttagaa
     8341 atgaaagcag agttggaagt aaatggtatt acagatgagt aacctaaacc aacacaactg
     8401 actgttaggc gctgaccgcg gaggattcgc gaagcagaaa tcctccgtgg tgctgtgcct
     8461 gttcatggac ggcgttagcc gaccatgatg tctcaccccc gtatctaaca ggggggtaca
     8521 aaaacacaaa aaataagtaa gcaaccatta agaatgcttg ttgtatcaaa gatagtaagt
     8581 aatttgtata ggtgttaact catttgaaaa agttaacacc ttgaatttgg aggtgagaac
     8641 ttggcacaac ggaatttaga ttatcggcta ttaaaagacc gacgcaacga atacggcgtt
     8701 tcacaaaata aattggctac ggcttgtgga cttagtcggc cgtacttaaa tcagatagaa
     8761 aatggtggcg taaccgcatc tataaaaacc atgaggaaaa tttttgacca actggaaagc
     8821 tttaatcccg atttgccttt gacgttactg tttgactatg taaggattcg ctttcccaca
     8881 acggatgcac gaaaaattat tcaagaaatt ctccatttga aatttgagta tatgctccat
     8941 gaagattatg ccttttactc ctatcaagaa cagtatgtca tgggtgatat tgtggtgatg
     9001 ttgtctcatg aagaagataa aggtgtgctt ttagaattaa aggggcgagg ttgccgacaa
     9061 tttgaaactt ttttactcgc tcaaaagcgt agctggtacg actttttcga ggattgtcta
     9121 aaggccggtg gcgtgatgaa acgcttggac ttggcaatca atgatcgagt ggggctattg
     9181 gatatacctg agctaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc
     9241 tttaaaagtt atcgttccgg ggaactttta aaggctgatg aaaaggacgg catgggcaat
     9301 accttatata tcggcagtct caagagtgag gtttatttct gtttgtatga aaaagactat
     9361 gaacaatata tcaagttagg gattccatta gaccaaactg agacgaaaaa tcgttttgag
     9421 attcgactga aaaatgaccg tgcttttcat gcgattcaag atttgttaaa aggtcgtagc
     9481 attgagagca ccacgttttc cattatcaat cgttacttgc gatttgcgga taaagtagaa
     9541 ggtaaaaggc gaaccaactg gcccttgaat gaacaatggg ggcgttttat tggacggaat
     9601 cggaaagaga ttcaattaac ttccgagcca aaaccgtata ccattgaacg aaccttgaat
     9661 tggctaggcc gacaagtcgc ccctacgtgg aaaatggcta aggagttgga tcgattgaat
     9721 cggacaacct atatccagga tatggtacag aatgcacgct tatcagaccg acataaaaag
     9781 attttggagc aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtca
     9841 tgggaattgt aaaagatatt tttttggtat ttggtggcag tatgcttggt gttactgtga
     9901 tgtgcttgat gaatgcaagt gcagcagcgg atcgagcaat ggaaaagaaa ggaaagtgac
     9961 aagatgaatt tcggacaaaa ttt
//
LOCUS       DAZUMB010000097         9966 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000097 DAZUMB010000000
VERSION     DAZUMB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.099"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..138)
                     /locus_tag="ACG80L_002119"
     CDS             complement(<1..138)
                     /locus_tag="ACG80L_002119"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5024331.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CM"
     gene            complement(284..1162)
                     /locus_tag="ACG80L_002120"
     CDS             complement(284..1162)
                     /locus_tag="ACG80L_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5024332.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1201..2319)
                     /locus_tag="ACG80L_002121"
     CDS             complement(1201..2319)
                     /locus_tag="ACG80L_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5024333.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2316..4415)
                     /locus_tag="ACG80L_002122"
     CDS             complement(2316..4415)
                     /locus_tag="ACG80L_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS5024334.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4466..5287)
                     /locus_tag="ACG80L_002123"
     CDS             complement(4466..5287)
                     /locus_tag="ACG80L_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5024335.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5284..6093)
                     /locus_tag="ACG80L_002124"
     CDS             complement(5284..6093)
                     /locus_tag="ACG80L_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5024336.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6114..6608)
                     /locus_tag="ACG80L_002125"
     CDS             complement(6114..6608)
                     /locus_tag="ACG80L_002125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5024337.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6620..7120)
                     /locus_tag="ACG80L_002126"
     CDS             complement(6620..7120)
                     /locus_tag="ACG80L_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5024338.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7185..9881)
                     /locus_tag="ACG80L_002127"
     CDS             complement(7185..9881)
                     /locus_tag="ACG80L_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5024339.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3015 a         2251 c         1645 g         3055 t
ORIGIN      
        1 catacattgg taagcaacta taactattac cacagacata aagtaacaca atatattact
       61 atttaagtgt acgccaccag tgaatatcct caaggtatac agcgcacaac aaaaagaaaa
      121 aaggaaacgc agaagcattt cctttcagtg atttaatgaa aacaacacga tttgacttag
      181 agccttttta tttgaattca ttttcgcttt atccaacttc atttgatcat ttgattactc
      241 ggttaaatac ttttgacatg agtatcaatg aaacattcat taattatggt tgcttatttc
      301 ttaccgcttt tctggccaat gctaatccgc cgattattcc ggcattatca cctaattcgg
      361 gcgtaataat gtagctatca attggtggta tttcaacata tccatttata agctcaacaa
      421 atttatcctg aacttttttc ttcatgtgtt cttgtttcat tacaccaccg ccaaaaataa
      481 ttctatctgg cgaaaacatc agtgttgtat tgtatgcaca ttgggcaagg taaaaggctt
      541 caatctgcca aaacgaatcg tcagccaata aattctggcc ttttacaccc aaccgttttt
      601 caatcgctgg tcctgctgcc atcccctcta aacaatcttg gtggaaggga cagtttcctg
      661 aaaaattgtc ctttggatgt cgcttgatca acatatgccc catttccgga tgactgaacc
      721 cttccacgaa agtcccattg aacaaggcac cacctccgat acctgttccc acagtgaagt
      781 atgcaacacc tgctaaccct ttgccagaac cagcaacata ttcaccatag caagccgcat
      841 tgacatctgt tgtccattca atcggaatat tgaaatattg ctttagcgtt cctacaaaat
      901 tgaaattttg ccaagcaagt tttggtgttg atgtgatatg accgaaactc gttgatttgc
      961 attgaatatc aattggacca aagcagccga ccccgatgcc tgccaattgt tccttaaaag
     1021 gtttaaaaaa atcaatgacc tgcttcattg tttcaactgg agttgttgtg ggaaaactga
     1081 ctctttccaa aatcgttaat tcctcagtac cgactgcaca gacaaattta gtccctccag
     1141 cttcaattgc cccatatatc atcataggtt cctcccttcg aatatgtgaa aaacacttca
     1201 ttaacgcggc aagcgttctc gatcagtgaa atgacgttta gtatttcctg aagcttctgt
     1261 tgaataccag taagcgacac tagcaacatc gtcttgccgc tcaaataagc gaacatcatc
     1321 gttgccgatt tgttgtaaag tgaccgataa atcttcggta aaacagatgg ggtcttgaag
     1381 atgccaacga taaagaccat gtcgtggcat tgcgtcgtca ccaaaggcat gaacggcatt
     1441 caattttccc gtttcaaaac gatctcttgt ggcatctcta tttgattggt aaggataccc
     1501 taaaaaggct gttgaatagt ttttcgcatg tggacgtccg aactcatcgt actcatgaaa
     1561 ggcccacgcc ccgccaaaat aatcttctga acctgttgag gttacggtcg gatactcact
     1621 atccccatca atgtagaatt taaactcgcc ttcgccccac caataacgtt ctaggcaggt
     1681 aagcgcaagg taagtaccca cgtaatagcc ttgacccttt aatcgatcaa ttaacttata
     1741 atcctcctgt aatttggtaa tgcgttcccg atgccaattt gcgtgaaaat ataggctatt
     1801 ttcttccaat tcgtccacca aagcgtaatt tatcgtataa aaaaatgagg acatatcctt
     1861 cggatgtaaa ttctcgatcg taattttagc ggatgtttgg aaaggcattt cgaaatagga
     1921 attaaagccc cctgtaggat taaccactat cggcaacgag ttcacctcgc aacgctctcc
     1981 aaacccatta caaaagaaat cccctaaagg actttcaaca gcagggctat ccgacccatc
     2041 ccaataaatt cttaagatta aatcacgtaa aacaaaactg ccaaattctg ttcgatcacg
     2101 aatcgtcatc cacatatgac gaattattcc cgttccagta atatccgcaa tcacagtttc
     2161 ttcaccagaa gtcagcggaa ttgcgccacg acctttgcgg cttggtccta gctcgcttgg
     2221 caacatcgca gctttacctt tctcgcctgt tggattttcg ggagtgattg ctcgactctg
     2281 gattttttta aattgggtaa tattttttag tatactcatc tttttctcct ttgaatgtga
     2341 atcgtttttc ccttttctac gtgaagaatt tttttcgtat cacttatttt caatggcttt
     2401 tctgacaaat ttgaaatact caactcggat tgcgtacatt caatcgaaat gaaggcttga
     2461 ccgaacttca aattcttcca agaaaacgtt aaattatcaa caggatcaat ttgcaatact
     2521 ccttttttga atttcaatcc gaaatattga ctgatcatta ggctgacaaa aagacccgat
     2581 gcccagccgc atttgccgca gccaaagtct cgcacgactt ctccttgttg agcctctaag
     2641 cgatacggcc accaccacca cgaaccgtct aaatctgcca aacaaattag ttcttctaat
     2701 cgggttacga aagcttgagg gttgtgcagc tcgctcatta aaacagtgat aaagccagga
     2761 aaagtggcgc cggattctaa tccccaagtg atgcctttga tttcctcgct ataggttgga
     2821 ttgaaagaac tagcagaaaa ggccatggtg ttctgatata aatgatcttt attatctaaa
     2881 aattgataaa aaggcatcaa cgtagtgtct gattcttctc catcatgcat caataaatta
     2941 atttttccat ccacaatcac atcacttaaa aagattaacc cttgctcaag aatcggtttt
     3001 tgatagtgct tcactgaata ggttgttttc tcctcatcac cgattccctc caaaaattgc
     3061 tcaccaaaag gaccatcgat agtcatttgt ttacaaattg attcctttag tttacaggca
     3121 atgtttttgt atcgcttgct aagactcagt tgttctaggg cttcaaaaat ttctgcaagt
     3181 ccaatacagg ccttccaaag acaaagattg ctgccggtat gatactttcc aagtgcaaag
     3241 gcgtctgata accaaacaga tggaaaaagc attggttccc ctgaccgacg ttgagctaat
     3301 atgtcctcaa taattcttga agcattcagt agaacctgcg gacaccgctc aaagaatttt
     3361 gtatccccag tatgttcaaa ataaagacta gccagtaacg cactggataa cgaattactc
     3421 agagaatgat tgatacctcc tgtaaatttt gaaccaggaa acttaacgcc atattgatca
     3481 aaccatagaa ccgttttttg acataactcc ggttcaaaca tggtgaatgg caatgatgcg
     3541 taatacatat ccttattcca aattcgatta gtagcaggat agctgcccca attgctgccg
     3601 acgacctgtc cctgtcgatt cataccgaaa ctagaaaagc tttgataaag tgcacgacga
     3661 tagagatgga caactctatc gtttggcatt gacagctgcc caaatagctg agcaaaatag
     3721 ttcaatgttt ccttcagcca tttttcgata tcagattcct gaaaagtcat tacttcttca
     3781 aaagcatttg ggtccacaaa agcaatggcg aagcttgcca cttcctgttg tgcgatcgtg
     3841 tccttatttc ctttttgaga aatcaacaca ttttgctgat ccgaatactt ctcttgatac
     3901 gaaggcacat ctttcactga aacttgaagg ctttcgtgtg tttcattcac aatgtaaacc
     3961 tgttgaacaa gcatacttaa acgcttatta gccagtatgg gagcaaagga tactacaatg
     4021 accttaaacg tcgaataatg atgaatatat ctaggcaaac agtcttgaat cacatcgact
     4081 tcaatacgat gatccgattc tgacaaatgg tagacaatcc cgtttacttc aacctttaag
     4141 gataacgaat tggtttgaga gagctgttta ttgacccaaa cacctggttt ttcctcagtt
     4201 agtaaatttt ctcgataaaa ggtcaacgtt tgaatcgtcc cgcaaacatc aaggtttgct
     4261 aatgtctttt gattacttac atcgaaggtc aatcccttta catcaggaat attccgatag
     4321 tagctgtatt cgtttgaagt agtcacattt ccgtcgttta actcagcatt aaaaaatggg
     4381 tgtttgggtg aataaattac ttcattaata gtcatctttt ccctccttgc taaaaaaact
     4441 gcagcctagt agctgcagct tcctactatg cgattccaag taatgacaat aaaattccca
     4501 agacgaccat tccaaaaata attttgatga cgccgacttt tttatctagt aatttgaaac
     4561 agctcaatgt taccaataac ggtacaattc ctttaaaaat actatcgagg atttcttgga
     4621 cattcattag ttcttcgccc ttcattgaaa agctcaaaac ggtattgaat ttgaccatac
     4681 tgatcgtcat tgcacctacc atgatcagac ccaagatact agcagctttt gtcagaattt
     4741 caattaaccc gttcgcatac agcttttcaa tatatttaga gcctaatgaa tagcctaaaa
     4801 cgccgccata gtatcgcatc aagtaattag gtatattgaa cataactaaa aagataattg
     4861 gtgccaaaat atttccggca ttggctaagt tgatcgctag tcccgcggta acgacacgcc
     4921 aaactcccca gaacaatgaa tcgccaattc cagccaatgg tcccatcaaa ctcgttttga
     4981 tcgcgttgat actttccacg tcaaaaccag ctttttcaga attttccttc tccatcgatg
     5041 ccgcaatccc catgacaaag gacgcgcaag gaatggtcgt cgaaaaatac tgtgtatgac
     5101 gttccagcgc cgcagctcga tcttcttcac ttttgtagaa atgattaatg aagggcatca
     5161 tagaataggc gaaaccaccg ccgcccatcg tcaccggatt caccgaagca tacatagtca
     5221 tcgagcgcca aaacatcttc cgaaccaatt ttttctcatc tttagaaatt ccatcagcta
     5281 atttcattga aagaaatcct cctcttcttg gtcaagcgtt gcatttacag cagcatcgct
     5341 ttgaactgca tgaacagata tattttttag ttgtagatct ctttgggcta taacaattgc
     5401 aataatcgtt ccaactgcgg caattgcaat caatggtaac tcgagatacg ccataacaac
     5461 ggccccaaag aaaaaccaca cagcaatatt tttactccaa agcatccgta acagcatcgc
     5521 catcccaact gccggcagca gatttccagc gactgttaat ccgttcatga tgacctctgg
     5581 aatattatcc aagacgcttt tgacaacatc tgctccaaat aaaacggcaa agaatggaat
     5641 aagagcataa agcgcatagt ggacaaacca cataaagaag tgcagattga tgattccttt
     5701 ttgatcacca gattttgcaa atttgaaaaa tttagcagcc atcggtccac atacaaaaga
     5761 aaaaatcata ttcattaact gtaatcctaa tactgctagt ggaaagacaa tgggaataat
     5821 cgcatccacg tttcctccca ttttgattgc gaacgctgta gccagcccag cagccaaaga
     5881 aggctccgcc gtactagaga ttccgatatt gacgacaccc ataaagatgg cttctagtga
     5941 cgccccgaca atcaaccccg tctgcaaatc ccccatcaac gctcctgcta acggagcaac
     6001 aacaatcgga cgactcagca tggacatccc gaacatttca tgtccagcaa ttccaataaa
     6061 aacggccaaa gcgactacaa atgcatcata cataattttt ctccttcctt tttctaaaaa
     6121 tcactcaaaa tagagatagc tgaacgtttt tccatcgccg gtgtaggctg aaaaacggta
     6181 tctggataaa gttcaactaa ttcttttaag tcttgaattt ctgacgtcgt taacatcact
     6241 tcctttgata aggttttctt atcttccgat ggcggtgtca ttttcccagc attccccacg
     6301 ttcattaatt cgatttccgt tgggaaagct ttcgcaactt ttaccgcatc tttgacatta
     6361 gggactaaaa ccatcaagcg catgtccttc gcccgcggat cttttaacaa atgaatcgcg
     6421 tcatcaactg ttttaataat gactttgaca ttcgatggtg cagccatctt caatgtcatc
     6481 ttttgaatat cgttggtagc cgcctcatcg cttgctacta ctaacccggt taaatttaga
     6541 ctctttgtcc aagtcatcgc tacttgtcca tgaattaatc gttcatcaat tcgcactgct
     6601 ttaatcattt ttcttcctcc taaaaaaaat cttcctcttc atttactaca tgtacctcat
     6661 caagaatggt catctttaat tcctgttggc atttatttac cagttcctga atatcctcct
     6721 ttgttaattc cttggttgcc aatactattt cgagcagaat tggcagatta aagccagaca
     6781 aaatagttac agcaatctct ttctccaaaa aggttttggt aatcttctga ttgacacttc
     6841 cgccaaataa atcggtaaaa acaaaaatct gatcctgctc gttcacctgg tttacaaata
     6901 cattcaactc ctgttcaaaa tcacactcat ccacataagc attaatcacc gtaatctctt
     6961 gaaccttccc cgtcagtaat tgaatactgc taagaaagcc tgaagctaag tgaccgtgag
     7021 tcgctattag aattttcttc atatggttaa ttcctttctt tctgttcact tttatataag
     7081 caattcctgt gccaactttt aaaaacattg gtttaatcac gttaattcac attaaaaaaa
     7141 gtgtctaacc taaaaaaaac agattagaca cttttttaga cactttattt ttgaatatac
     7201 ggtcctaata attcatagag cagcgaaagc tcataagcat tgactttaaa attatatttc
     7261 atctccattg attgaaaaat acttctagaa acataataaa attcatcttc atctggtttc
     7321 aaatcataat tcggttcttc agatttttct gtccgagcca agaataaacg ttccatcatc
     7381 aatgccgtat gcatatagag atttagtttt acacggccat ttaaagtgaa atgatagtaa
     7441 ttttcatatt tagaaatgac tgtttccact tcactcatca cgatcgttgg gttcagaaag
     7501 ttcaaacggt ccgcaattcc ttccaacgaa aagaatttta ctagctcctg aatcaagcgg
     7561 ttaaattctg ccggcttcat ttcaggactc aaaatatgct ccaaattggc tgctccatca
     7621 ccgtctaaga tgtcataaac attaatatta ggaattgaaa aggaacgtgg aagatccgaa
     7681 gtcgtgatga ccaattttgt cttgtcaaaa taagtttcat cattttggtc gattaattca
     7741 cgtagttctc tgtagtcctt cgtgaaaact tcgatggatt catccgataa atgatgacga
     7801 aaaacatctt tcaatttgtc cgaaatcccc aaacccgaca tacaggaaat gataatgttg
     7861 ctactttgag aaaagccttc aaagtattga acattgattg aatacttgtc tgctgcatcc
     7921 tccgcaatct gtttaaatgg cgcattcatc gtcatcttca agccaatatc aagtgcaatg
     7981 gaagtcgtca aattattcag aagaagtaac tcccctttta aatcgttctt gatctgtgtg
     8041 taaatttgat ttaaagagcc catatctacg atcagcacta gactatctgt attcacttgc
     8101 cgtttcacga aattccttgt cgattcgaca atctctgaca cattactctc tactggcata
     8161 tccaacgctt caaaaacata attgccgcat aattgattca ctactgcttg aatgctgcta
     8221 gccgtactat ctccgtgagc aagcaacagg cccttcagct cgatggattc atctacataa
     8281 tcagataaag tcacgacaat aagcagtctt agaaagttct cgtccttttc tgagagcggt
     8341 ggtaaatgct ccaagaactg attacataag taagtcgtcc gaggtagatt tgcagctaat
     8401 gtacgattta tcccggttaa gtctgtctta tagcttgggg aaatgacata ggatctgcta
     8461 ataatttctg caacaggtcc actcgtcgtc aatccgtacg tttttcctaa aacggatacc
     8521 cagacctttc taaacatctg aatcatcaat tttctactca ttgataaaaa agaatcctta
     8581 tcaatgtgat gcaccaaatg ctgtacttca ttctttatcg aagaaaaatt ttcattgaaa
     8641 ttaccttcat ttatcagctg agtaattttt tccaaaatcg aatcataatc aaacggtttg
     8701 attcgatccg tttttatctt ttgatttttc tttatcaata aactagcaat cggcaagctt
     8761 tctacttcgg attccagcat atttaactga atcaagatct catttttccc aatcgttaat
     8821 ggttcttctt gtgttcgact atacacatcc gcacaactga ttttaatgat attcttcagc
     8881 ttaccaatat tcccactaaa gggatgagat aacattaaag acaaggcttc tcttgaaaca
     8941 ataatatctt tttgtaaaac atccgcttca ttttgaaaaa ataattgaat cagctctaat
     9001 cgttctcgaa tggggcgatc ttcaaaagtt ggcaacataa cactaatttg aatacgccga
     9061 tcaaaggtgt ctaataaata attttcacct ttttccgtcg tggcgaaaat aaaacgaacc
     9121 ttagcagaat gccaatttac attttcaccg actggacgat agtttccagt atcaataaat
     9181 agaaagagct tttcttgatt ttctttcgat aggcgatgga cctcatcgag aaaaagatag
     9241 ccgttgttgg ccgttgccaa taaaccatcg gtatcctttt cagcccccgt aaaggccccc
     9301 ttcttgtatc caaacagtgt tgccgaaagc agttcgggat tgtccgcata atcggcacag
     9361 tttaagatag caaatggtgc atctttttca ataatttgct catggatagc gtattcaaaa
     9421 atttttgtgg ctaaaaaact ttttccaaca cccgtggctc catttattaa tacactgagt
     9481 ccgttaggcg gatatttgac agcggctata catttttgaa tgacctctct cagacttccg
     9541 tcatgaccaa ctacagaatg aaaggcttcg tttatttgct ccgtattatc tacaaccgca
     9601 tattcatcta aaggcttcca caaaacaggc tttcccggta atttctcgac ctttttatct
     9661 tccactaatc tcgtcaaata atggctggca ttctgacgag agatacctag attcttcgaa
     9721 attttctcgg cagtgacaga agacatattc cctgttagtc gtatcagctc atcaaaaact
     9781 cgatctttag cgctcttttt cattttttcc tccagtttaa tgtgtctaaa agcataataa
     9841 actatcggct gcaatatgtc tattctatat ttgtattcca atttttactt tgaaacacaa
     9901 atagtcgaat atttgcctca gatatctgac aaaataattt atctaccaat ttagttttta
     9961 tctaat
//
LOCUS       DAZUMB010000098         9906 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000098 DAZUMB010000000
VERSION     DAZUMB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.100"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..139)
                     /locus_tag="ACG80L_002128"
     CDS             complement(<1..139)
                     /locus_tag="ACG80L_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5024340.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     IL"
     gene            complement(273..1463)
                     /locus_tag="ACG80L_002129"
     CDS             complement(273..1463)
                     /locus_tag="ACG80L_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFS5024341.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(2335..3966)
                     /locus_tag="ACG80L_002130"
     CDS             complement(2335..3966)
                     /locus_tag="ACG80L_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS5024342.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3980..5653)
                     /locus_tag="ACG80L_002131"
     CDS             complement(3980..5653)
                     /locus_tag="ACG80L_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS5024343.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5666..5983)
                     /locus_tag="ACG80L_002132"
     CDS             complement(5666..5983)
                     /locus_tag="ACG80L_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5024344.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(6008..7408)
                     /gene="lacG"
                     /locus_tag="ACG80L_002133"
     CDS             complement(6008..7408)
                     /gene="lacG"
                     /locus_tag="ACG80L_002133"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS5024345.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7645..8430
                     /locus_tag="ACG80L_002134"
     CDS             7645..8430
                     /locus_tag="ACG80L_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5024346.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8433..9125
                     /locus_tag="ACG80L_002135"
     CDS             8433..9125
                     /locus_tag="ACG80L_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5024347.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9399..9770)
                     /locus_tag="ACG80L_002136"
     CDS             complement(9399..9770)
                     /locus_tag="ACG80L_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS5024348.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2993 a         2152 c         1734 g         3027 t
ORIGIN      
        1 ataagattct caatgcagta agagcattgg ttttccctga atcattctca cctataatag
       61 tatttgtctc attatcgaat cgaaagattt cggattcata atttctaaaa tttcgaattt
      121 gaagagtaga tataaacata agcagatctc ctaactattt aattaatatg attatatagt
      181 aagtttatca taaattatgt tagtaaaagt tctcattata agggagcgtc aataattttg
      241 tgtaaatgat tcttccctac tgcagattgt ttctaattct caatcagttg gcttagcatt
      301 tgttcgagct cgccttcggc ctgttggaaa cctttatgac tgcgacctag gaatttttgg
      361 ttgtaagtat caaaagacga gactaagaaa cgttccatcg actcttcatt ttggaactgt
      421 tccttgcggt ggctgtattt cttgatttgt ttattaaagg attcaatcaa gttcgtagaa
      481 taaatactct tgcgtatggc caaggggaaa tcatagaaag tgagcaagtg atcgttcgaa
      541 agaatcgatt caaccacttt tggatagctg gttttccatt tttccgcaaa agtaccacgt
      601 gcttccaacg ccacctcttt agatgaggct tgatacacca ttttaaaatc atcacagact
      661 tccttacgat catcgacacg cactttatga ctgatattac gggaaacgtg tacacaacaa
      721 tgttgaaaac gagctttggg atagaaccga ctaatcgctc ctaccatccc ctttaagcca
      781 tccgtgatga acaggaggac atttttcaaa ccgcgctctt gaaggtccaa taaaatttcc
      841 tcccaaatcg tgatggattc agtcggtgca atcgcatagc tcaatacttc ctttgatccg
      901 tccgggcgaa tgccaactgc gatatgaata gcttccttgg cgacggtttt ccgctttaac
      961 ggaatatacg ttgcgtccat ataaatagca gcataacggt catgaagctc ccgcccatta
     1021 aacgccgtta cctcttctgt aaatgatttt gttatattgg acatggtttg gggcgtgtag
     1081 taatgcccat acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg
     1141 aagaggtgaa tgacggtctc ctctaacgtg tcattcgtcc gtctataagc aggaacagtc
     1201 tgttgcttga actcaccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg
     1261 gtcttgaccg tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga
     1321 tcatactttt cgtaatctag gaaagccgtt aactccgttt tgagaagcgt attgactgct
     1381 ttctccaaat ggacacggaa taattcattc aaatcgcctt tgttcgctag agtctttaga
     1441 atttctgtag taaaatcgtt catagggaag tcctctcttc tgtgaatgtg ttgtggtaac
     1501 tctattctac agaagggact tccttttttc tatttacaca aaatatttta cactctccat
     1561 tataacaatt gggttaaaaa gaaaatagat agtattctac ctcatattta tttgaaagta
     1621 atatataaaa gacgttcaaa ttcgaacgcc tctcgttaac caaatattgg attcagaatc
     1681 tagttttcat atataaacag agtatgatta ggtgcaaaaa ttgcactttt attgcacttt
     1741 tatttttatt tatagtgttt tactgttctg tatattgttt taactccctt gatagtatcg
     1801 cattctgttt tatacacttt acgttgaaaa cagagtggga ataaagagcc aacataatat
     1861 ggagggggaa tccctataaa tcaacattct tcaatagcta aaatgcttga ttttcattaa
     1921 aaataagatg gttccctacc aaaagagact ataatataag ttaatttgtt tcattcttat
     1981 cataaaaaga agttctcgaa aaacgaactg ttttccgaga acttttttct atccatctaa
     2041 gtgctcaaat catattcacg acggaattct aaggttaccc tttcttaaac aaactttatg
     2101 ttatctgcac aactttttgc aattttttca ttgcttctta cgaaatcgtt gaactctctt
     2161 tagcataata caaagtgtag atgaagtctg gtgctttact cgttttggta aaaatgactg
     2221 gaaatcgttt ctggttattt ttggcaaagg cgaggtgcag gttcgattcc tgccttctac
     2281 gctaacgatg tggctgaagg gaacctccga tcttgaatcg aaacgatccg cagtctattg
     2341 tttcaccaat gaaacaaaat caacgttcgt ccaagcattt cctgaagcct tgctataaac
     2401 cccaatttca caaacacctg ttgtaattgg aatgtctttg attgtaattt ttgtccattc
     2461 acttgtttta acaaccgcag tatttagttc cgctccacca taatttttga tatataactg
     2521 attagtatca aactctcccc cagacattac ataggcactc aatgtgtaag ttcctttgct
     2581 tatgccacta acattttgat aagctgatgc ctcgtatgct tgatcctgcc aaaaagttag
     2641 tttgtagtcc ccaaaaaaag tgcccgcttc cgttttgatc gtacccttag cggttccttg
     2701 ttgaaaccag gtttgccaat tggaaggact ggtcgttggt ccatcgcttt cgaaacttgc
     2761 atttttaact agatttattt gcgcccctgc ttgtttaaac gcacgcaagc ttggcaaggc
     2821 gtttccagca aaatcaaaca tcgcttggtt gtcccattcg tttcccatag tggattgaac
     2881 actgagataa ttcatacctg cttgtgttgc ccaactcaca tttccgttgt accaaagagg
     2941 ttcccaatag aagaagcccg ccccgcgatt attcggtact gcctgaatcc gatcggcaag
     3001 atccagcaaa tactctgctt gtcctgtcgg tgttggttca tatccccccg cagctgcttc
     3061 ggcttcacca aaagcatttg gcatagtatc ggcattcgct gttgtatgcc cataagctgt
     3121 ttcaactacg atcacttctt taccataccg ttggctgatc gcattcatat tggtttgaag
     3181 ttcattcatt gaaccgtgcc aatacggata atacgacact ccaattaaat caaatgacac
     3241 acctcgactc gtgatttcat caaaccacca agtaaatgct cctgtatcgc caccatcagc
     3301 taagtggagc attactggag ttgttttagg ttgagtgtct cgaacagctt ggataccaga
     3361 cttcaaaaaa gcagccaatc gatcaaattc tcccccgcct tctccccaag acttgccata
     3421 aggccagagc attccgctat tcagttcatt gccgatctga accatatcag gataaacgtc
     3481 cagctgcttc atttccgaca gaacgttctt tgtatagtcg tacaatgccg tattcatttc
     3541 ttcaaaggtc agaccttgcc atgctttagg aagattctgt ttcccaggat caacccaaaa
     3601 atcactgtag tgaaaatcca gcagcacctt aagtcccaga ttttttgctc gttttgcaag
     3661 gtcaatcgtc gtttccagat catttgtccc tgctccataa ctttctccat tactattata
     3721 aggatcctgc cacaaacgca gtcgaacata gttgacacca ttatttttta aaatctctaa
     3781 ggcatctttt gaaatatcgt cttcataata ttttgcacca gatttttcca tatctgacag
     3841 gatcgaaata tctgctccgc gaataaattc gctggcagat gcttcaacag tttttgaaac
     3901 agcccccaaa gcccctaaca cagctgcaaa taaaacagct ttacgcaacc aaattcccat
     3961 tttcattatg gctcctcctt tattgaaatt ggttcagaat aaactgaaca gctttttcag
     4021 gatctctcgt taatccgata tattctgctc cttttgtcgc aaccagcttg attcctaagc
     4081 gatcggtgtc ttctttgata tcatcatagt aagaattgac ttgcggtgct aagaccacca
     4141 tatcaaaatc ctgcagcatt tcgtaatgag aaccataagc acccgccatt gcactgatcg
     4201 gtgctttcga ttcagatgca ccttttttta aagcattagc taacatcgca cttgtccctg
     4261 cgccagcaca aagtaccaac acattgattt ctttatttgc atcgctgata acttgactcg
     4321 ctgccacagg tacttcctca cgtacatcgg cagaagcacc ttcctcggtt gcgatcacct
     4381 ctgctttttg tgcttctttc tctactaaga cagcatcgta tgctttgcaa aatggtagat
     4441 agatcaggaa gtcaacgacg attagaaccg ctgctagaac aaaagctaag atacccatgc
     4501 ccgttcctag aaccaaaccg atcggtgctg gagttgccca aggcaaaacg tacataaagc
     4561 tgttcatgcc caatacatcg acaaaaaact tgaacaggca aacgtttaca acaggtgtta
     4621 acaaaaatgg aataaaaaaa taagggttca aaatcatggg tgccgcaaat agcaatggtt
     4681 cgttgacagc gaaacttact ggaataaagg aagctttccc aactgccttc aactgcttag
     4741 acttagcaaa gagtaggaag ataaaaggaa caaccaatgt tgccccagtt cctcccatag
     4801 ttcctacaaa attccctaaa cccaccgtca gtactttaga agcttgttca ccggaattga
     4861 acaattgcag attggtttcc acattggcat agataattgc cgcaattgcc ggctccacga
     4921 tcgacggtcc atgaacaccg acaaaccaaa acagcgccat cgctccccag ataatagcga
     4981 ttcctaagta cccgtcagct gctgtaaata gcggctgcag caccgtaatg attgcttctg
     5041 caaatgagat ttggaagatt tgtcgaatgc ctaaatcaat taaaacggcc gcaaatactg
     5101 aaaaagagaa aggaaaaata tctcggaagg tttgcgaaat cgttccagga acttctttag
     5161 gcattttaat ggtaatatct ttcagcacac aaaacttata taaattaact gttaaaaatg
     5221 cagcgacaaa ggaagccagt agtccctttg tccccatata ttccgttgaa aatccacctt
     5281 caatcggaga gacgcttaac aacaggaaac ttaccacaga ggccagcatc acagaggtgg
     5341 tgttgattac tttgtttttc ggtaagcgac gattcatcga atcagccaag cagcgagcag
     5401 tggttgctga aacgagtaac cctacaacac ccatcgaata attatagact ttccatagcc
     5461 atgtggagac agattcaggc cattggtaac caaaaatctc aggaaccgca gcaatcaaaa
     5521 tgaaaatgct tgaaaataaa atagctggca tcgctgttaa aaatccgtca cgaacggctc
     5581 ctaaataact attacgtgag attttttcaa aaaatggttt ccctttttcg atttgtttaa
     5641 tgattccatc catagcgttc ttctcctatc cttgattctg tctgtacagt acaataaagt
     5701 cttgaatcag atctctcagc aataaagtgg tcattaaatg atcctgccca tggataaaaa
     5761 tgaatcccat ctccatctct ttcccagatg cttcttccgc taaaattttt gtttgcgaat
     5821 tatgtgcaag ggttaaattt tcgtcggctt ctttcaacgc cgaatcaaca tgttcaaagt
     5881 ttccttgtct tacttctttc aataatttca ataatgtact gcgagcgtcg cctgaatatg
     5941 caacgatctc aaaaccaagc atttgtaatt cctctttatt catcttttct tcctccatta
     6001 tcacttatta aataatcgtt ttggtttcac tgaccaactt gtaccagtaa gctgattctt
     6061 ttgggtaacg cttctgggtt tcaaaatcaa cataaaacaa gccatacctt ttgttatacc
     6121 catttgtcca tgagaaaaga tccataagcg accacaaaaa ataccccttc acatttaccc
     6181 cggctgttat cgccttgctt aaggattcca aatagactct caaatagtcg attctcggct
     6241 gatccataat aatgccatct tcaaactgat ctttataccc catcccgttc tcagtgatat
     6301 agattttgtt gtaatgtggg taatcgcttt taattcgcag caacaagtca tatagacctt
     6361 ctggataaat cagccagtcc caatcagttc gaggaattcc ttctttataa atacgttctc
     6421 cgattccctt cactttataa acagaggtcc ctttttcacc cgtaccgtta tgatggattg
     6481 cattttctcc atcgtaagct ttgacaaaat ggcattggta gtgattgatc cctaagtagt
     6541 cattacgcgt ggatgctttt ttcaattcaa cgaaatcttc ttcaggaaaa tgatagctgg
     6601 cctgatttgc ttcacatatc tcatcaagag cagtcaaggt ttcagttgaa taatagccta
     6661 aataggtagc atctaataag aaacggatcg acaatgcatc gtctaaaaag gcagcatgct
     6721 tatcttccgg cgcgtctgtt gccgcatatt tcgtctctaa tgagtgaact acaccaattt
     6781 cacccggaag ttcgttttct ttgaaatagt tcactacacg ggcatgagca accatcatat
     6841 tatgaagaca ggcgacgatc tttgtaaaat catatttgat tcctggtggg aaaacaccta
     6901 acaaatattg attggttgca actggataga tttcattaaa ggtactccac accttgactt
     6961 ctttgaattc atgaaaacaa aaaatggcat aggaaacaaa tgcttctatt gtttctcgat
     7021 tcaaaaaatc tccatggtca aacaaacgtt taggggtatc aaaatgatgc aacgtcacaa
     7081 acggcgttac gtttcttttc ttacattctt cgaacacacg atgataaaaa gcgattcctt
     7141 ctggatttgg tttcccagct ccatcaggaa aaatgcggct ccaagcaatg gataatctta
     7201 gaccatttac accaaaacgt tcacacaatt caatgtcctt tgcatattga tgataaaaat
     7261 cacttgccgg atcgggactg aatcgccctt gttcctctaa aaagtcatcc caagcaacag
     7321 cgcctttgcc accttcctta gtggctcctt caacctgata ggctgctgtg gctccgccaa
     7381 aaataaaatc ttctggtaat ttaagcatgt acatctctcc tcaaatctat aatcagtttt
     7441 cgctctttct aactgattgc attgtaaacg catccaaaac atttgtcaac aaaaacaaac
     7501 acaaataatc ataaaaacca gaaaacagca ataaaatcta ttgtaacaaa cataaaaacc
     7561 accctaaatg tttacttttg ttcgaattag attgactgtt gtccgtttat atgatagatt
     7621 atccttaaat tagaggtgaa gacaatgttg aaaaaagaaa gattgcttac tatcgtagaa
     7681 atggtcaata aaaaaggaat ccttaccgtt aatgaaatca tcaatcaatt agatgtttct
     7741 gatatgaccg ttcgcagaga tttagatgaa ttagaaaaat cgggcaagct gctgcgtgtc
     7801 catggagggg ctcaaagtct ttcctacact ttagatcaag aactttctca cactgaaaaa
     7861 gccactctgc agattcaaga aaaaaaagaa attgctgcca cagcagcctc cttgattcac
     7921 gagggagaaa cgattttctt aggaccaggt acaacaatcg aactattggc gacgcattta
     7981 ctgaacaaga aaattcggat cattacaaat aattatccag tttttgatat tcttgctcaa
     8041 gcagatggac cagaaattct actgattggc ggtgatttcc ggaaaaatac cggcgcattt
     8101 gtcggaccga tcaccaacga caatttaaga agactaaagt ttacaaaagc atttatcagt
     8161 tcaaatggcg ttcacaacga agcgatctct acttacagta ccgaagaagg agaagcacag
     8221 cagatcgctc taaataattc ccgtaagaaa tatctactcg ttgataataa aaaattcaat
     8281 cgagatgatt tttatgtgtt ttataatttg catgatttcg atctgctcat tacggatcgc
     8341 cagatctcgc gggaggttca agagcactat gcacaatatg tcgatctgca aattgctgat
     8401 tcaaccgaaa ataccactat aggagcgtaa ctatgaaagg gattatattt gatttcaacg
     8461 gaacaatgtt tttggactct catctgcatg aagctgcttg gctgcatatg atacatgatc
     8521 atacaaaaga tggtttatca gacgaggata ttttaaaaaa tattcatggt cggacgaata
     8581 cggagatttt gaaacatttc atttcagagc atttgaccgc ctctgagatc gccgtattgt
     8641 cagaagaaaa agaaagctat tatcgtatgc tttgcttgca aaatgaaaac gagcttcagt
     8701 tgacaaaagg gcttgaagcg actcttgacc agctgactga ccaacagatt ccattaacga
     8761 ttgccacggc tactgtcaaa gagaatgttg ctttttattt tgatattttc gatttagccc
     8821 gctggtttga ctgggacaag attgtctatg acgacggctc tttccctggg aaaccgcaac
     8881 cggatatttt tcttaaagcg gcagacaaac tgagcttagc tccggctgat tgtcttgtta
     8941 tcgaagatgc ttactcagga ttacttgctg ccaaacgtgc ggggatcgga acaattattg
     9001 caattgatcc attcggtaaa aacaaaacag tatttgaaca ggcacagcta aataaagatg
     9061 gaaccatcaa agattttact ggtttttgga cagagcacct gtctgccaca tccgttaatt
     9121 cttaaacgat tcaactaact accaaagaaa agcaactgaa atgcacacct ccttttataa
     9181 aaaactgcca gtgaaacgac atgaaagata cactgctata tccttagtga aatatagcag
     9241 tgtatctttg actttttcat tcttttaacc atatgacgcg ttcatctcta tctataatta
     9301 acattaaaaa tagtgttttc acattatttc tcaaaccaat cttaaaaatc atcaggagta
     9361 cttttttgat atacttttat taatgctggg cgtaatggtt aaaagatttc cactagttcc
     9421 tctccaccaa gctgagttga aaaatgctta tctgtaaaca acatagacaa agaggaaata
     9481 aacgtttccg tatctttctc tgtcccaagg atattaatta attttcctac taggtaagta
     9541 tcattaaact taagatctgc cttcacatca aaagttttga taaaagcttt tatcatgcgt
     9601 acaattttgt ctttatcgcc ataattgatt tcaccactca tatcaatata gtcactaatc
     9661 cctgaagaaa taatggcaac tgcttcttct ttcgccttat cagataattc atttaatgac
     9721 tgggattgat tgatgacttc ttgagtttta tctgttacgt aatctctcat tatcctatcg
     9781 gcatactccg caaagtaatc ttgaaaaaat tcttctacag ggtaaccaac ttcgttttcc
     9841 caaccaggag aaatttcatt atcagtactt attaaatcta ttaaaaacca acccgttctc
     9901 tgagtg
//
LOCUS       DAZUMB010000099         9901 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000099 DAZUMB010000000
VERSION     DAZUMB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.101"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..684
                     /locus_tag="ACG80L_002137"
     CDS             205..684
                     /locus_tag="ACG80L_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS5024349.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="ACG80L_002138"
     CDS             717..2945
                     /locus_tag="ACG80L_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5024350.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="ACG80L_002139"
     CDS             3074..4717
                     /locus_tag="ACG80L_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5024351.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="ACG80L_002140"
     CDS             complement(4790..5335)
                     /locus_tag="ACG80L_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS5024352.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="ACG80L_002141"
     CDS             complement(5358..5843)
                     /locus_tag="ACG80L_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5024353.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="ACG80L_002142"
     CDS             complement(5833..6645)
                     /locus_tag="ACG80L_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS5024354.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="ACG80L_002143"
     CDS             complement(6784..8316)
                     /locus_tag="ACG80L_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS5024355.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="ACG80L_002144"
     CDS             complement(8425..9456)
                     /locus_tag="ACG80L_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5024356.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3296 a         1834 c         1905 g         2866 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttatt ctatggtagg agcaattata ttgagaatta
     9901 a
//
LOCUS       DAZUMB010000100         9573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000100 DAZUMB010000000
VERSION     DAZUMB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.102"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..203
                     /locus_tag="ACG80L_002145"
     CDS             <1..203
                     /locus_tag="ACG80L_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024357.1"
                     /translation="KFGINFSKYLKKVRISKAKLFLTETRLTLTEICEKVGYTELGYF
                     SRVFKEETGMTPSQYRKGTFVK"
     gene            200..1936
                     /locus_tag="ACG80L_002146"
     CDS             200..1936
                     /locus_tag="ACG80L_002146"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5024358.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(1999..2835)
                     /gene="pheA"
                     /locus_tag="ACG80L_002147"
     CDS             complement(1999..2835)
                     /gene="pheA"
                     /locus_tag="ACG80L_002147"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS5024359.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(2832..3341)
                     /locus_tag="ACG80L_002148"
     CDS             complement(2832..3341)
                     /locus_tag="ACG80L_002148"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS5024360.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(3334..4629)
                     /gene="aroA"
                     /locus_tag="ACG80L_002149"
     CDS             complement(3334..4629)
                     /gene="aroA"
                     /locus_tag="ACG80L_002149"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS5024361.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(4642..5733)
                     /locus_tag="ACG80L_002150"
     CDS             complement(4642..5733)
                     /locus_tag="ACG80L_002150"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS5024362.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(5750..6916)
                     /gene="aroC"
                     /locus_tag="ACG80L_002151"
     CDS             complement(5750..6916)
                     /gene="aroC"
                     /locus_tag="ACG80L_002151"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS5024363.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(6918..7982)
                     /gene="aroB"
                     /locus_tag="ACG80L_002152"
     CDS             complement(6918..7982)
                     /gene="aroB"
                     /locus_tag="ACG80L_002152"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS5024364.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(8015..9037)
                     /gene="aroF"
                     /locus_tag="ACG80L_002153"
     CDS             complement(8015..9037)
                     /gene="aroF"
                     /locus_tag="ACG80L_002153"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS5024365.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         2607 a         2133 c         1780 g         3053 t
ORIGIN      
        1 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
       61 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
      121 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
      181 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
      241 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
      301 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
      361 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
      421 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
      481 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
      541 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
      601 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
      661 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
      721 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
      781 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
      841 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
      901 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
      961 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     1021 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     1081 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     1141 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     1201 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     1261 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     1321 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     1381 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     1441 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     1501 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     1561 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     1621 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     1681 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     1741 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     1801 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     1861 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     1921 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     1981 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     2041 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     2101 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     2161 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     2221 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     2281 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     2341 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     2401 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     2461 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     2521 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     2581 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     2641 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     2701 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     2761 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     2821 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     2881 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     2941 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     3001 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     3061 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     3121 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     3181 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     3241 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     3301 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     3361 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     3421 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     3481 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     3541 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     3601 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     3661 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     3721 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     3781 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     3841 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     3901 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     3961 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     4021 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     4081 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     4141 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     4201 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     4261 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     4321 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     4381 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     4441 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     4501 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     4561 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     4621 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     4681 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     4741 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     4801 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     4861 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     4921 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     4981 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
     5041 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
     5101 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
     5161 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
     5221 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
     5281 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
     5341 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
     5401 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
     5461 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
     5521 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
     5581 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
     5641 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
     5701 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
     5761 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
     5821 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
     5881 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
     5941 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
     6001 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
     6061 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
     6121 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
     6181 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
     6241 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
     6301 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
     6361 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
     6421 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
     6481 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
     6541 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
     6601 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
     6661 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
     6721 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
     6781 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
     6841 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
     6901 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
     6961 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
     7021 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
     7081 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
     7141 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
     7201 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
     7261 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
     7321 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
     7381 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
     7441 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
     7501 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
     7561 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
     7621 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
     7681 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
     7741 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
     7801 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
     7861 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
     7921 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
     7981 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
     8041 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
     8101 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
     8161 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
     8221 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
     8281 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
     8341 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
     8401 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
     8461 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
     8521 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
     8581 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
     8641 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
     8701 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
     8761 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
     8821 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
     8881 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
     8941 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
     9001 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
     9061 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
     9121 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
     9181 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
     9241 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
     9301 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
     9361 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
     9421 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
     9481 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
     9541 actttgtttt tcagaaaaat aacaattggt ttt
//
LOCUS       DAZUMB010000101         9497 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000101 DAZUMB010000000
VERSION     DAZUMB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.103"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..289)
                     /locus_tag="ACG80L_002154"
     CDS             complement(<1..289)
                     /locus_tag="ACG80L_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS5024366.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEEL"
     gene            complement(279..2453)
                     /locus_tag="ACG80L_002155"
     CDS             complement(279..2453)
                     /locus_tag="ACG80L_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFS5024367.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2469..4811)
                     /locus_tag="ACG80L_002156"
     CDS             complement(2469..4811)
                     /locus_tag="ACG80L_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5024368.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4833..7376)
                     /locus_tag="ACG80L_002157"
     CDS             complement(4833..7376)
                     /locus_tag="ACG80L_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5024369.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7386..7784)
                     /locus_tag="ACG80L_002158"
     CDS             complement(7386..7784)
                     /locus_tag="ACG80L_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS5024370.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7865..8149)
                     /locus_tag="ACG80L_002159"
     CDS             complement(7865..8149)
                     /locus_tag="ACG80L_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024371.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8173..9183)
                     /locus_tag="ACG80L_002160"
     CDS             complement(8173..9183)
                     /locus_tag="ACG80L_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5024372.1"
                     /translation="MAFLMSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDR
                     MIQFSQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQ
                     TLSSTEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVA
                     KDVRFIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLN
                     LSESKNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKG
                     LEGGELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTL
                     NE"
     gene            9310..>9497
                     /locus_tag="ACG80L_002161"
     CDS             9310..>9497
                     /locus_tag="ACG80L_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5024373.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTK"
BASE COUNT         2716 a         2022 c         1498 g         3261 t
ORIGIN      
        1 gaagttcttc ttgtcttggt tcttttcttg tttcaattcg atatacattc ctagagaatt
       61 ttttgtcgta tctttccaag aaagattcaa cttcttggca ttttttacaa tcatttcttg
      121 ctagatacac aaaaaatgat tgattcgttt tttgaagttc tagtacttct tctaaattac
      181 tattgattag ttttccattt gattcttcgt tagtttttat cagtttaaaa caaccaaata
      241 agaggaaact aacaataagt atcctaacaa taactttctt atttcgcata ttgtttctcc
      301 aaatttggtt tccgaatata gacgactcgc ggtggttcct ggacgtcttc agctgttcga
      361 tacaccacaa attcgcctgt tctcaatgct cgaatgcgat tcggattgaa tttaaagcgg
      421 tctacgtctc gtttcgtgcc tctttctcct gtccaatcaa cctttcccat atcagaataa
      481 cctgcttctt gtgtcgtcat ttccgttaat tcaatgtctt cttctgtccc cattaaatct
      541 gcccaataat caatttctgt cgggtcattt gtcttcccaa taaagaacgt attcacattc
      601 ccaatcaact gttttcgaag gacaggatca attctatcaa tgtccgcaag cgtttgaggg
      661 gcatacgccg catgcacccc agcgcctcgt cccttattga ctaagtctat aaggttatca
      721 tttagataag aagctggttc gtcaaatatt actaggactt tcgagcgatt ggctgtttct
      781 cctgcttgtt tatacacttc tgacgcaaag aaattaatat ctcccacaag catttgagcc
      841 aatgtccgaa taaactcact ataaatcaat ccgtttaacg agatataaac aatctttcct
      901 tctttaacaa cttcttgaat atctaattct ctttttcctc cagaagtatc aaagagttca
      961 ccaagctcac tatccaataa catattgata ttggtctgta aattctcaga agtcgcatac
     1021 aaataaaacg ggttcttctt atgttcgtaa cgaacaaaca accgtttaaa caacgtttgc
     1081 tcgatttctg tcatgagaga aatgttttct tttaataata gataatacgt ttctaagtca
     1141 acggttttac gatcatgttc aacgattcgc tcttcactta actgttccac atcggcaaaa
     1201 tcacgttctg ttttgtccat cgcttcattc tttttcgttt ttttcttcgt ggttttcttc
     1261 ttttctacga ccactttttc tttaaataaa tctggatttt tcaactgaat acgatccgca
     1321 aacaaatgta agacgtttcg tggcaataaa taatgttgta accaaactag atttctttca
     1381 attttatggc tcttttggaa ctcgtctaaa agctgaatcg ttacctgtaa taacgctttg
     1441 gctgctgtcg aataatacac gctttctgtt tctgctagat tggttaattt atctcgaatg
     1501 gcaatatcat tgccattttt tacaggatta taacgcaaat cgccaccgtc cacaaagact
     1561 tcaatcgggc gattgtatgc ctctgccact tcttttaacg cttgtaaagt ctctcctgct
     1621 cctttcccat cgactaagat aacggggata cgaaacttac acgcatgttc tgctagtaat
     1681 tggatcaccg tcgtttttcc tgaacctgta gtcgctggaa taatcacatg ttggttcaat
     1741 tcactaaagt tcatcacata agggagccca tactcatctg ttcctaatag aatcactttg
     1801 gctaatgaag ccagttgttt attccgcaaa gcaacgtctt ttatgacaat tgctacctgg
     1861 tacgcttgct gatactctgc tgtatatttt tgggactcct gaaatttatt cttccgacgt
     1921 tccttgtatt ctttactatt tagcaaagct gtttgccgtt ctttctttga aacaactttt
     1981 cttttttcaa taaaagacgc atacgcaatc aaacaacttc ctgcacctgt cccacctaaa
     2041 aggagggcaa aaatcgtttg tccattaaac tgcaacgcat gagaaaacaa gtttaatgga
     2101 aataataagt aacacgacaa ataccctcta ggaagaacaa aaataaaaca aaatagattt
     2161 aatagcgtaa aacctaacgc aaaaataaaa aaaagagagc ttcttcgttt taagaagctc
     2221 tcatactcaa agtcataaat atgtgcacgt gtctcttttt tatctatcat aaagacaatc
     2281 gctaaaggaa tactgcctac aataaaaaat ggatatagac aaacacctaa taaagtgacg
     2341 ataaaataaa gttgttgaaa ccgacttttt ggcgtttctt tttcttgttt tcttttgcga
     2401 tcgacagaaa acatttgctc aaaatgtgtc ggtcgttttg gtgcattcat catactatcg
     2461 atttcctcct atcgttttcg ttgtttcatt tctcttcgat aaatcggttg tttttctcgt
     2521 tttcgtttca tttttgcttg agttgctcga aactcactcc gtgtcttcgg tgcttgtttt
     2581 ggtgattttg tttgtttcat ggttgagtat gctttagata cggttgaagg cgctttggtt
     2641 tgtgctgtta ctcgattgac tgctgacata tgttcccgaa ccccctctgg acgtcgaaca
     2701 gatttcggtg tggtagaacc tgttgctcgt ggtacattcc cttttcttag tggtttttgt
     2761 accgcatgtt tttgtcctgc aactgtccgg gttttcatcg aacttgtttt tgttacggga
     2821 gtaagtgcct ccttccgacg acgacttgtt tgtccctgac gacgttttcg atcgttcaat
     2881 tctgttctag gcgtatgctt ctggccataa gacttgtgtt tttgctttgg attcgctaat
     2941 gacaccacca ttgcagaacc ctgattgcga tacggactac gagacgcacc ttttctactt
     3001 ctttctctta gactaggatt gccttgcaat tctcgttgta ataaatcggc tgtttttctt
     3061 gcctgtttct ccattgctgt tcgttgcata cgttcttgac tttgctcttc tgcttgtttc
     3121 gcttgatcat gcaagtctct tgctttttgt gccatacgtt ccatacgatt tggtgcttta
     3181 tgacgaggtt gttgtgcgga ttcacgccga aaagcacttc gtgtggcttg tttcgccgtt
     3241 tgtggcatac gaacgtctga gccaccccca cgttttcgtt gacgttcttg tctcttccgt
     3301 gcgttttctt tttctttttg tttctgtgct tttctcgctt ctttgcgttc tttcaaccgt
     3361 tgtttggctt cccttctcaa ttttgcttct gccccaaatg gatttcccgc aaaagctaac
     3421 gctcctcgaa cacccattct tccagtaaat agactgccta aaaagcctcg gaacaaataa
     3481 attcccaacg gcgcaaacaa ataaacaatg atcaagagaa ttttttccca tggatcgtct
     3541 tgtgtactac ttaattggaa cccgtaacta agaaaagaag aaaaaaggat acatgcaaaa
     3601 cctgtcattc cttttaaaaa cgtcaaaata ccaaaaactt tggcatagtt accaaacaca
     3661 ttcattgtga caagaaacaa tcctaaaaaa agtaagaacg gcaacatcgg tactaacaat
     3721 agttgcatag ttccaataat caatcgaaac atacttaaga caaaataaac aacggtttga
     3781 atcagattca cgacaaaata aaacaaggca aatgctgtat ttttccaact attgtaatat
     3841 tgtatgttct tgtttttcag tgtatctgct tcatactctg tgactttgtc atgaattttc
     3901 tcccctattt ttgtacctaa atcattgtcc aataaaagat tgattcgatc atattctttt
     3961 ccagcatatt tgactttctt ctcacgaatt gtgtctacat tggttgtgcc ataatttaac
     4021 aacagatatg gttcataaac attactataa aagacccgag ccgccatcaa tttgcctgcc
     4081 gtttccactt ttttatccgc tgttacctct tctgtttctt ttgtttcata atttgtttct
     4141 tcttcaggtg ttaacactgg attgaccttc acgaacgccg tttccactgt agaatctaaa
     4201 gaaaaaagaa catcaaaaag attcttattt gtcccagaat cttttaatac gcttaaccca
     4261 gtaaaaataa ggatcgtcat aagaaagaca cggagtgctt gtttcattcg agcctctcca
     4321 gcatatttga ttgccatcat aattgccgaa aaactgattc caatagttcc tgcaatactt
     4381 aacatattgt ccgcaatttt ttgtgttaag ttttgaatgg gttctcgaat ggcggaaata
     4441 atatctaatt caaagagaaa ttcaaccatt gaagcattga attctcctaa catttttacg
     4501 ccactccaaa caaaattttt catcactcct gcaacattgt acgatagctt ttttttaagg
     4561 ttcattgact catagtcatt ggtcatcaat tcaaacgaat tctttttgta atttttatac
     4621 aattgatcca catcagtcgt tacttctttt acttctactt tatcgttggc attctctcct
     4681 tcagaacgat cggtttcagt gacacccgca ccatcattca tttgatcggc agaaacagta
     4741 gccccttgta gacagaaaaa aagaccccct aataaaagga gcgtccacca tttcagagat
     4801 ttttttcgca aaaaaaccac ctcttttttg ttttatcgtt gtttttcatt ttctaacatt
     4861 gcttgaattt tttcatcttg attttcagta gaagaaacag ccaataacca actatcaaac
     4921 aacacatcaa tggcaatcgg ttgatttctt cccaaatgat cctggaataa acaggttcct
     4981 gtttgtaatt gttccatcat ttctaaattg gctgaattca cttccatatc aaagaaggac
     5041 aatagtttct ctcgtgcttc tttttgtttc ggacgaaacg caaatttgta agaaattaac
     5101 tcttttttat cttcttgatc gtaatcttta aacgcttgca caacaaggta aatatccgta
     5161 ttattcgcac gtccttttcg caagctatca tcaatcaaat agcttccttg tgcggtatct
     5221 tcataccctt ttgcctcgtc ataaataatc gcagcgtctt cttctttgtt tgtagaaaag
     5281 atatacgtaa acttcataat gacttccata attgccattc ctgcaagttg ttcgctattt
     5341 aatcgacccg aatcaatctc tttttgtgtc ggaatgcgta ttccttgtgt ccctaacacg
     5401 ttgatttgtg aatcaaaacg tatcggcgta ctgtgctcat tcccaatgag aatctttcct
     5461 aaaccaacgc tatgccctaa aatcaattta gctagttctt tgtcactttt tcgaatttct
     5521 tcgataactt gacttaacga atgcttacct ggcatggtgt aaactacatc gatggcgtct
     5581 tggatcaccg ttttcttgtg gctcgctgtt tctcgattgg tatctacttc tcctaacatt
     5641 cctaacacat ttttagcggt ttgaattgcc tcttcttttg gtaaaaacaa tagaggatca
     5701 agcatccctc gacagctttc atctgaagat aacgaaataa aattaattcg ttcatacata
     5761 gccacaaact ctggtatgtg tccgaatttt tcaagtgcct ttaaaaagaa cttttgggtt
     5821 tcatttttcg gatcaatata aagaattttt tgacctaaaa acatcagcca taaaaaaata
     5881 tattttacaa ggaccgattt tcctttacct ggtggaccaa taatcaacgt atttccattg
     5941 gtatgttccg cgccttcaat acttttcttt gttaaattgg gataatacca tactagttta
     6001 ctagatagcg ctaacgcttc tttcgcagat ttaaactttt ttgcaataac aatccgtcct
     6061 aaaggaaatc ctttcttatt tcctacttct ttctttaagt ccaagccata atctgccaca
     6121 aaacctgtcg tagcgacctg ctcgtaacta cgaaaacgtt gcgtacttcc aattaagcat
     6181 tgattaaaca acgttagctg atccgctacc gatcgataaa atttgaaatc tgtagactca
     6241 atcgcaaatt caatatcttt gactcgttct tccaattctt cttttgaaga agcagataca
     6301 accagcgtga cggtagaata acacaatcgt ttttctttgc gcttcagctt atcttttaca
     6361 tccattaacc gttctgtacc aaaatttaat acatcgtctg aatctaaaat gccgtcaacg
     6421 gtctcatatt ctttttcttg ttcataaatc cgtccagcca ttttattaat ttttcgttca
     6481 tcagattttg aatgctcaaa tcgaagtcga atatgcgtat caattggaaa agataagcta
     6541 tcctgaagat tttgaataaa gctactccca tacatggcta aaggagcgtc cgtttgactt
     6601 aaaaacgtcg aataatgcgt ttgattcaac tgttcaatcg caatgtatcc tgcttcattc
     6661 gaaatgacgc cctcgcaaat ttccattggc gtaagctgtc tttcaggcaa tttttcttct
     6721 gatcgatgga aaaaataata ataaatttgt ccaatctctt gggcagataa gcgctctatc
     6781 ggcttataat tggacaattc cgtatagagc tccttttcta atgtttcata cgttggtaac
     6841 acactactta taggaactcg ttgtcccgta atacgataga taaacttttt aaaaacatgc
     6901 ttaagcaatt gaaaatattc gatcggatca gacactttca tttctgtagt gaaacgcaca
     6961 aacagatacg ttcgatactc gtttaattgc acttcgtctt ttaaaatctt tcctgcctgt
     7021 tcaaagtaca ctttcccaag atctgcgcat tctcccttta ccaaatctcg aatggtttct
     7081 tcaatgtgcg tatctaggtt aaattgacgt ggaatattga taaaatgata gtcatattca
     7141 tcatgtccaa gtatgccttc gccatcttga agatagttct tgaaaaaatc aatgtcattt
     7201 aaaggaaata cttgggaaga aagttgatag cctacccaca catcatcatt tttggtcaaa
     7261 acaaaatttc ggataatggc gtctatcgga aattctaatc gaggaagctt cgtttttttc
     7321 tttttgaact gccaaatccc gataataaag acgaccaaaa gaaacaaaaa cgccatttac
     7381 tcgactcact ccttccattt gtgtagggaa tacgtcactt tttcatggaa aaacggcaca
     7441 tacatttcat gttcaaagcg ttgtttccgt ttctgataat aggacgttcg cccaattaaa
     7501 tactcgaaaa agtgtttatt gtcccatttc attgaaaaca atgtccaaac gagaacacca
     7561 atcccaaaaa tcataatcaa ccaggacttt cgaaacagtg tctgcaagaa actaatttga
     7621 ttcacatacg caatcaaaaa taggatcaaa agaatgaaga gaaatacccc acctatgact
     7681 aattgttcca tgcgaatgcc attagaaatc cagggaattt tttgtccttt aatcccgtaa
     7741 attttgtgag ggtgcttaaa ctcctttgta tagttgtatt ccatgttcac tctacctcct
     7801 tttacaaaaa aagaagcgac cagaagttct aatcttctga tcgcttttgc agtttgtctt
     7861 tttcttatga taattgtatc cagggcacaa tcatttgcgt tacccccacg ttctcttcac
     7921 tatccttaat aaagataatg ttctttagcc attcaccaat atcaacggca ttcgataaca
     7981 ataagacgag cattatcaaa ataaagacgc caataatggc aaacgctgcc cgcgcaaagc
     8041 cttgggtcgc ataagcacca accattagaa caatcgcaac gattgccaat agccaaatga
     8101 cttgtccaga tacatcatca aataacgatt taagatcaaa aagtttcatt tccattctcc
     8161 tctaagttca ttttattcat ttaacgtagt aacaaaataa ctatcgccac ttgtttttac
     8221 ttccattgaa aagtttgatt ggaactcgtt tcctttctca aaatagaaac gatacttccc
     8281 ttgcaaaaag tagtttcctt ctttattttt tactgcatga tccagttgta cattgatcaa
     8341 ttcgccgcct tctaaccctt tttcgttaga aatcaatgcc aatttttcat catttttgac
     8401 atataactca aaaaaccgtt tcacaaatgc ttccagcttg atttcttcgc tttcagtgac
     8461 cgcttgccca gaagaataaa atcgattttc ctgatagttc acagaattgg tattcttact
     8521 ctcagaaaga ttcaaaaatt gaacgccatc aagtactttt aaaacattgt tttgataact
     8581 gactggaaaa gaaagttgac tgcttttcca atgttctcct tctgtaaaag taacctcata
     8641 aaataggcga tacattgctt gtgtcaaatc gatttcttct agtttaataa agcgaacatc
     8701 tttcgctact cgattggtag ccgtcgttac aaagcctaga tcagacgctt ccaatccttg
     8761 tgctaaataa tgattgacgg aagcctccca ttcgtttcgt ttttctggcg aataagtaaa
     8821 taacctttca ataaaatgtt gtccctcgta cttcaattgt tccgtagaag acaaggtttg
     8881 gttgacttca gttaacaatg tttctggatt gacttgtttt ttcactgcta aacgcgttaa
     8941 tgtatccact cgtccaaagt ttaggaggag cacgacaccc attaacagaa acaaacaaaa
     9001 aaaagcgcct gaaaatacac tcatggcact tttttgcgaa aattggatca ttcgatctga
     9061 cggttctgtt ttcttttttt ggaacaaaaa acgtttcttt tgtttctttt ccttctgttg
     9121 tttcggtggc ttagtttgga acgcttgtgg attaaatttt gcattactca tgagaaatgc
     9181 cataccgttc taacaataat ttcggtattt tgtccacaat cactacttcg tggacaacca
     9241 cacttgcccc ctccaatgat gtgcacccca aaagttagac tagaaatcta attaaaaggg
     9301 gtgctttttt tgcggaaata tacatttgtt tttaagaaaa aagtagtttc agactattta
     9361 aataacgaag gcggctacaa atatcttgca cataaatatc aaataaatcg tacactggtt
     9421 agacattggg taaggattta taactatcat ggttgggaag gcttggttgg aggtggcaaa
     9481 agctacacta caaaatt
//
LOCUS       DAZUMB010000102         9415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000102 DAZUMB010000000
VERSION     DAZUMB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.104"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..1133)
                     /gene="recA"
                     /locus_tag="ACG80L_002162"
     CDS             complement(84..1133)
                     /gene="recA"
                     /locus_tag="ACG80L_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS5024374.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1242..2483)
                     /locus_tag="ACG80L_002163"
     CDS             complement(1242..2483)
                     /locus_tag="ACG80L_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS5024375.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2723..3301)
                     /gene="pgsA"
                     /locus_tag="ACG80L_002164"
     CDS             complement(2723..3301)
                     /gene="pgsA"
                     /locus_tag="ACG80L_002164"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS5024376.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3510..4433)
                     /locus_tag="ACG80L_002165"
     CDS             complement(3510..4433)
                     /locus_tag="ACG80L_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS5024377.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5172..6458)
                     /locus_tag="ACG80L_002166"
     CDS             complement(5172..6458)
                     /locus_tag="ACG80L_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5024378.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6451..7719)
                     /locus_tag="ACG80L_002167"
     CDS             complement(6451..7719)
                     /locus_tag="ACG80L_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5024379.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7971..8432
                     /gene="mscL"
                     /locus_tag="ACG80L_002168"
     CDS             7971..8432
                     /gene="mscL"
                     /locus_tag="ACG80L_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS5024380.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8492..8938)
                     /locus_tag="ACG80L_002169"
     CDS             complement(8492..8938)
                     /locus_tag="ACG80L_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5024381.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9031..9276)
                     /locus_tag="ACG80L_002170"
     CDS             complement(9031..9276)
                     /locus_tag="ACG80L_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS5024382.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         2618 a         2032 c         1663 g         3102 t
ORIGIN      
        1 acgatttctt tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca
       61 gccgtttttg tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt
      121 tcatcttctg ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa
      181 acttctgcca tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga
      241 ccaatccgat cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct
      301 actgccatgt ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact
      361 tcagcgattt tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta
      421 ccaacaatat ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt
      481 gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct
      541 actttttcac ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca
      601 gagagtttac gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc
      661 atttcgccgt cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata
      721 tctacagccc cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca
      781 ggctgtgaaa gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct
      841 aacgcatgct cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata
      901 gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt
      961 ccacgggggt aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt
     1021 gtagagattt gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc
     1081 ttttcaattt ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata
     1141 atcctcctta tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag
     1201 tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt
     1261 tttctcaaaa gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg
     1321 gcaaagtgat atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt
     1381 cctgcagatt gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa
     1441 tctgttcttg ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact
     1501 gttccatatt ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc
     1561 gagtaggtaa cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg
     1621 gcttggaagg cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc
     1681 agtagttcca ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc
     1741 cctactcgtt cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca
     1801 tcatttgttt tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc
     1861 gttggattga tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca
     1921 atcccataaa agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct
     1981 agtaaaggac gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga
     2041 aggatatacg catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt
     2101 ggtagaggaa ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt
     2161 cgatgggttg tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga
     2221 gacttaccga ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat
     2281 ccgccacata agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa
     2341 cgctgctgat tatctcctac aactgtttga taataaactt cgatacctag atcagctagc
     2401 tgttctgaca agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg
     2461 acagctatta tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt
     2521 tatatttttt tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct
     2581 tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa
     2641 aacatgaaaa gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca
     2701 caacctctcc actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga
     2761 agtaatccac acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta
     2821 ctggaatacc gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca
     2881 tttgtgtagc cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga
     2941 caagtagtaa acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc
     3001 aaccaggggc ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat
     3061 ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt
     3121 ttccatctaa ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg
     3181 tgacagccag tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa
     3241 ccaaaatgaa aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca
     3301 cactatcgac tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt
     3361 atttactttt ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga
     3421 attaacgaag aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag
     3481 taaacagatg cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca
     3541 ttttttggac tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg
     3601 atcgttgcat tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac
     3661 acggtactct gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact
     3721 agcacaccta accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg
     3781 tctgtgatat ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc
     3841 ttttcttcac ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt
     3901 tgctctgagg aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct
     3961 ccgattattg gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc
     4021 gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc
     4081 gattcttcct gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca
     4141 gggaccgatc ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct
     4201 tctcctacag cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat
     4261 tggtcaaaat catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc
     4321 tgtaattcat ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg
     4381 gtttcaaaag ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt
     4441 tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt
     4501 ccgaataaac attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata
     4561 taagaagctg ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac
     4621 ctctttctta aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa
     4681 tataaggaga tatttttccg tatgtttctc taatactcag gtcaaaacgc tgatttcaca
     4741 acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta
     4801 aaaacattaa gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt
     4861 tcacaacctc ttggtacacg gtgttccaga agtagcttct tcggcaataa gccgaaatat
     4921 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
     4981 ctgtcacaac ctctttgatt acttcgtttg attttatttt aatataagtt gattaaaaga
     5041 gagcatgatt tgttttattt tctcactaat ttttccaaca caactaagaa taccataaaa
     5101 gctgattttg gaggaggttc ctactacaag tctaaaacga aatttaagac ttttcctcat
     5161 aaaatactgg tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat
     5221 gcttccccag ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca
     5281 aataaagttc gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa
     5341 tttaatgatt ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct
     5401 gacacttccg gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc
     5461 tctggttcgt ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat
     5521 tcaaaaccaa agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa
     5581 gtattgccta aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct
     5641 gttccttctt gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta
     5701 atggctgctt ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt
     5761 acaatttctt gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc
     5821 aatttctcag gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac
     5881 gtccgatagc acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc
     5941 gcaatatcga tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc
     6001 cgccagttag ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg
     6061 tttacagttt cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc
     6121 ttttctacct gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca
     6181 ttggcggaag ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac
     6241 aacttatgtt ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca
     6301 tagggaataa attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg
     6361 tgatagtcat ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa
     6421 agtgtttcat tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa
     6481 gaagattgcc tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct
     6541 ctccatacgt ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa
     6601 aaaagaagct tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag
     6661 cattgctttt gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa
     6721 ttgctcgtga atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac
     6781 actcatgaat cctcggaacg tatccacgct actagaggca taataagcta gactttcttt
     6841 ctctctgaca ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac
     6901 cattagcgca aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag
     6961 ctttgcttga acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg
     7021 ggtataaaac agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc
     7081 ttcagctgct ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc
     7141 catcatttct tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac
     7201 tacgcccaac cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc
     7261 ataggaagca aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt
     7321 cttttccaag tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa
     7381 aacctctttt aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc
     7441 attcacaaaa ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa
     7501 tccaaagctt gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccgaata
     7561 attcaaacta tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg
     7621 ttgcagacgc gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa
     7681 gacatgaaga ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca
     7741 gttgattgcc cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct
     7801 tgaaatttat aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta
     7861 tagcatagat gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat
     7921 tttttatttt tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag
     7981 aattcaaaga gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg
     8041 gtagtgcgtt tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag
     8101 gttggtttgt cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg
     8161 actggtcgcc gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta
     8221 catttctgat cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga
     8281 atttacgctc caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc
     8341 ttagtgacat acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag
     8401 agcaaacaaa tctttccgat acttctaaat aataaagaat ctaaaaaaaa agaaagccga
     8461 ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt
     8521 cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact
     8581 cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt
     8641 tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt
     8701 cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt
     8761 aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttct ccatcaatgt
     8821 catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga
     8881 cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta
     8941 agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg
     9001 aaccttacga atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc
     9061 aactgtccga gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc
     9121 aggatccaaa tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc
     9181 tgtttgacgc tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc
     9241 taaatctttc ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta
     9301 taccacatat ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat
     9361 aaataagaag ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaag
//
LOCUS       DAZUMB010000103         9357 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000103 DAZUMB010000000
VERSION     DAZUMB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.105"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG80L_002171"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG80L_002171"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS5024383.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG80L_002172"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG80L_002172"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS5024384.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG80L_002173"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG80L_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS5024385.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG80L_002174"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG80L_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS5024386.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG80L_002175"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG80L_002175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS5024387.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG80L_002176"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG80L_002176"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS5024388.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG80L_002177"
     CDS             complement(8053..8523)
                     /locus_tag="ACG80L_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS5024389.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG80L_002178"
     CDS             complement(8569..8994)
                     /locus_tag="ACG80L_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS5024390.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG80L_002179"
     CDS             complement(9061..9330)
                     /locus_tag="ACG80L_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS5024391.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3286 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttt
//
LOCUS       DAZUMB010000104         9182 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000104 DAZUMB010000000
VERSION     DAZUMB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.106"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..2838
                     /locus_tag="ACG80L_002180"
     CDS             61..2838
                     /locus_tag="ACG80L_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5024392.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2919..3338
                     /locus_tag="ACG80L_002181"
     CDS             2919..3338
                     /locus_tag="ACG80L_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5024393.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3350..3820
                     /locus_tag="ACG80L_002182"
     CDS             3350..3820
                     /locus_tag="ACG80L_002182"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5024394.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3837..4616
                     /locus_tag="ACG80L_002183"
     CDS             3837..4616
                     /locus_tag="ACG80L_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5024395.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4603..5424
                     /locus_tag="ACG80L_002184"
     CDS             4603..5424
                     /locus_tag="ACG80L_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5024396.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5441..6490
                     /locus_tag="ACG80L_002185"
     CDS             5441..6490
                     /locus_tag="ACG80L_002185"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5024397.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6503..7507
                     /locus_tag="ACG80L_002186"
     CDS             6503..7507
                     /locus_tag="ACG80L_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5024398.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8140..9159
                     /locus_tag="ACG80L_002187"
     CDS             8140..9159
                     /locus_tag="ACG80L_002187"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5024399.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3058 a         1538 c         1942 g         2644 t
ORIGIN      
        1 tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg
       61 gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc
      121 gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta
      181 aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa
      241 atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag
      301 ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg
      361 caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc
      421 gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt gattacaggc
      481 gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat
      541 gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat
      601 cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa
      661 gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta
      721 catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt
      781 tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg
      841 actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc
      901 gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt
      961 tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca
     1021 cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt
     1081 tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat
     1141 catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc
     1201 tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag
     1261 cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta
     1321 tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt
     1381 gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag
     1441 acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc
     1501 ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt
     1561 atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac
     1621 tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg
     1681 gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg
     1741 ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta
     1801 aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca
     1861 cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg
     1921 atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta
     1981 tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa
     2041 tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag
     2101 ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc
     2161 ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg
     2221 gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt
     2281 gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc
     2341 accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta
     2401 gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc
     2461 attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag
     2521 gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc
     2581 agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc
     2641 cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa
     2701 gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc
     2761 cccaattctg agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg
     2821 atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc
     2881 ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag
     2941 cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa
     3001 atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag
     3061 taaggcagct ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg
     3121 gcggaagtgt caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg
     3181 ccggattgaa tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga
     3241 caactgtttt gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg
     3301 agcggctcaa tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt
     3361 gacaagagtc gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat
     3421 aggcgcagac tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac
     3481 aacgatcaaa ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc
     3541 gattgccgcg ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc
     3601 gatcgaggat gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg
     3661 agggatcaag gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga
     3721 agaggaggaa cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt
     3781 accagcagat aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga
     3841 ttgtacaagc agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag
     3901 ggacatcgtt gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg
     3961 atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct
     4021 cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg
     4081 ctattacagc tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt
     4141 tgacattgat attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag
     4201 cagacaatta tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg
     4261 gtatcggtct ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca
     4321 atacgatcgg aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag
     4381 ctacagggtt gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac
     4441 aagttgcacc atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga
     4501 ccggagtagc tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc
     4561 gtcaactacc acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc
     4621 aaaaacagca aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg
     4681 gagttttctt ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg
     4741 attttgaaaa agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta
     4801 gagtttttta acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg
     4861 gaggaagaaa ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca
     4921 tctttgatgg gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg
     4981 attgccacag gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg
     5041 ttcattttga ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga
     5101 tatcgcttag gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg
     5161 acattaggcg cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc
     5221 accatcaata ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt
     5281 tttgatgata tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta
     5341 ttgaagaaag aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt
     5401 ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta
     5461 tatctatgaa gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag
     5521 agaacatatg aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc
     5581 ttgtttagca gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga
     5641 acctttttat tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt
     5701 gtcacaaagc gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg
     5761 gttacctgtt gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca
     5821 aattattgat ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc
     5881 gactgtttta cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag
     5941 caaaaaaata gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc
     6001 gattattcaa aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca
     6061 gcgtttcgtc accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa
     6121 attgactgaa acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg
     6181 cccatacctg gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa
     6241 taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac
     6301 cattaccaca aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga
     6361 atatctgtct gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt
     6421 agctaaaggt attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag
     6481 taaattataa gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat
     6541 aaaaggcgca ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga
     6601 aaaatttgac gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc
     6661 tgtcacttat atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata
     6721 ttacactact ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac
     6781 cggtacaact caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact
     6841 gattggattt atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac
     6901 ttatccagct cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta
     6961 tttgaatgga gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc
     7021 aacagggtta gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa
     7081 acatcgacgt gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta
     7141 ttcatatgcc atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc
     7201 tgccgagttt cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt
     7261 tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat
     7321 ttgcagcaat tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa
     7381 atatcgcgga cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc
     7441 tcatgtggag cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga
     7501 ttattaggat agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc
     7561 cctttatttt tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc
     7621 tacagttcaa cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg
     7681 caatagtact atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc
     7741 gccagtttac atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa
     7801 ccgtcaacag agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg
     7861 tctcttatgt attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa
     7921 tgctggtagt aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg
     7981 taaaaataaa atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa
     8041 agaagtaaac aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact
     8101 ataatggaaa ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt
     8161 cttggtcctg gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat
     8221 gtccgtattt ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat
     8281 cagcattttt tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca
     8341 gaagcagtgg ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca
     8401 gtcgcacagg aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa
     8461 ggactagaac aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa
     8521 gaaaaaagac aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta
     8581 catgatatca caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa
     8641 aaactgttca tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa
     8701 actggtgcag cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga
     8761 ttcggagatg atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt
     8821 ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg
     8881 atcgttactt gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag
     8941 ggacataagc tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg
     9001 acaaaagcag cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca
     9061 atctataatg ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta
     9121 cgtgacggaa aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc
     9181 gt
//
LOCUS       DAZUMB010000105         9076 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000105 DAZUMB010000000
VERSION     DAZUMB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.107"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..851)
                     /locus_tag="ACG80L_002188"
     CDS             complement(27..851)
                     /locus_tag="ACG80L_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS5024400.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(824..1504)
                     /locus_tag="ACG80L_002189"
     CDS             complement(824..1504)
                     /locus_tag="ACG80L_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5024401.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1527..2465)
                     /locus_tag="ACG80L_002190"
     CDS             complement(1527..2465)
                     /locus_tag="ACG80L_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5024402.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2710..3561)
                     /gene="ispE"
                     /locus_tag="ACG80L_002191"
     CDS             complement(2710..3561)
                     /gene="ispE"
                     /locus_tag="ACG80L_002191"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS5024403.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3715..6288)
                     /gene="mprF"
                     /locus_tag="ACG80L_002192"
     CDS             complement(3715..6288)
                     /gene="mprF"
                     /locus_tag="ACG80L_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5024404.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6429..7235
                     /gene="proC"
                     /locus_tag="ACG80L_002193"
     CDS             6429..7235
                     /gene="proC"
                     /locus_tag="ACG80L_002193"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS5024405.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7314..8870)
                     /gene="rny"
                     /locus_tag="ACG80L_002194"
     CDS             complement(7314..8870)
                     /gene="rny"
                     /locus_tag="ACG80L_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS5024406.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2670 a         2011 c         1564 g         2831 t
ORIGIN      
        1 tttataaggc ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt
       61 cgtttgatga tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct
      121 ggaggtgtat ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca
      181 caaacactga tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg
      241 ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata
      301 gccacaccag taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg
      361 tgggcggtat cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat
      421 ataccgatgg caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg
      481 atcgaaccaa acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc
      541 aatactaacg ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag
      601 gttcggtaga tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg
      661 gtcgtaagtg taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca
      721 tgagaaagcg tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc
      781 ggggcgattc cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa
      841 agcaacgcca ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt
      901 tgatatcttc atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat
      961 gtcggagaag acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat
     1021 caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt
     1081 gtccgccaga caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta
     1141 aagcacgctg tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg
     1201 gatatcttcc tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg
     1261 caacttgttg aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa
     1321 tctttatcat tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact
     1381 tggctgctcc attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga
     1441 catcttctag tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac
     1501 gcaccttaca acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt
     1561 ttttccatga cagaaatata attttcgcct gctttgattt gttcattagt caagctttct
     1621 aaaggattca acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc
     1681 gaagaggcat tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt
     1741 agctcagcta agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact
     1801 tgttttaagt cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg
     1861 cggtttgttg catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg
     1921 atataagcat cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat
     1981 tcttcagcaa tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga
     2041 tcgtattcat gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc
     2101 gattctaaac ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact
     2161 gaatccttta acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct
     2221 gcatcggaaa tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca
     2281 gcagggatca aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac
     2341 atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta
     2401 tttccacatg ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt
     2461 ttcatttata tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact
     2521 aatgtatcaa aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg
     2581 tatattcttg attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta
     2641 caataaaaaa ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact
     2701 tcttatattt tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc
     2761 attatatact ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct
     2821 tccactcatc aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat
     2881 gactggatgc cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg
     2941 ctgataatct tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc
     3001 ttgcgtatct acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa
     3061 cacaacccag cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac
     3121 aaatgccgta cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc
     3181 tgccagttct tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc
     3241 tgcacagtct gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa
     3301 gatcttgaca ccggttttga tattgtattt attttttagc aataaagcag cttgataaac
     3361 attgttccgt tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc
     3421 tgttatttct tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa
     3481 ctcatgatac ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg
     3541 cgctttttca atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct
     3601 atcataggga aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt
     3661 tcaacagctt tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa
     3721 ttctgtgagc acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact
     3781 gagtagctgc atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc
     3841 ttcccaatga tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa
     3901 attaccataa ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc
     3961 tccaacatta gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta
     4021 tacaataaac agattggcta acagatagtc agctaattcc tcagattctt taggtaagac
     4081 ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac
     4141 gactttttga tctttacgga caataccgac accgctagat aatacatatg atttatcaaa
     4201 tcgtcccaca gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt
     4261 tacagcttga tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt
     4321 catcaaacgt tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc
     4381 aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac
     4441 gtagctttct gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat
     4501 aaactgattc gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa
     4561 gcatttgttt cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta
     4621 ataaaaataa ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt
     4681 ttccaataaa cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc
     4741 agctaaatat tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc
     4801 tgagaaccac acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac
     4861 taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa
     4921 gctagcatca aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt
     4981 tctggataac cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa
     5041 cgtacgtgtg attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc
     5101 ctttttgact tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag
     5161 attcactgtc tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact
     5221 gattgcagac agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac
     5281 agcacttacg agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa
     5341 aaatcgattg attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg
     5401 tagaacataa taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac
     5461 agacaattga ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac
     5521 catcgtcagc attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga
     5581 aatatctatt ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg
     5641 tccaaaggat gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt
     5701 ctttctcttt agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag
     5761 ccagatccaa tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt
     5821 aagaaacgac catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc
     5881 taaaaccatt tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac
     5941 cacaccacca aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa
     6001 ctcttttctt tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc
     6061 atacaacaac atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat
     6121 gcggctttgt tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt
     6181 tgcgacttga tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag
     6241 actatgttcc tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc
     6301 ctaacttgta tacttttgtg tcatacccca atgttatctt atttatttta atttttccac
     6361 tttagacctg ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag
     6421 aggataacat gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg
     6481 gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg
     6541 aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag
     6601 ttgcatcaca atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat
     6661 tggaagaact aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat
     6721 cattaataga tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc
     6781 cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca
     6841 cggaggaaca gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt
     6901 tagcagaatc agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt
     6961 acatgctgat cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag
     7021 ccactgagat tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata
     7081 aaaatccatg ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg
     7141 tagttgcctt agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga
     7201 tcgagaaaga tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt
     7261 acaaaaaaag aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta
     7321 gcataatcag ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc
     7381 agttcttctt cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag
     7441 atttcttctg gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat
     7501 tctacaccgg cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt
     7561 tctaatgatt cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca
     7621 aggacagaga taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt
     7681 actactgggt tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct
     7741 tcaatctcat ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat
     7801 tggatatctt cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa
     7861 tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt
     7921 agatctggat gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga
     7981 atacgttcat ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg
     8041 attcgtccat cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga
     8101 tcgaatccgg aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc
     8161 agcgtctcaa gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca
     8221 tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt
     8281 tgaatcgcta atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt
     8341 tcagattctt ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg
     8401 atgacatctc gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga
     8461 tcttcaatca ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta
     8521 agtgcttctt ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga
     8581 ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt
     8641 tctctcagct cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac
     8701 aatgcttctt tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct
     8761 gatgattgtg caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa
     8821 ccaagaccga caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc
     8881 tccatactat ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac
     8941 tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg
     9001 tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta
     9061 aacgatggtt tttaaa
//
LOCUS       DAZUMB010000106         8851 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000106 DAZUMB010000000
VERSION     DAZUMB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.108"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1277
                     /locus_tag="ACG80L_002195"
                     /pseudo
     CDS             63..1277
                     /locus_tag="ACG80L_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1785)
                     /locus_tag="ACG80L_002196"
     CDS             complement(1456..1785)
                     /locus_tag="ACG80L_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5024407.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1775..2038)
                     /locus_tag="ACG80L_002197"
     CDS             complement(1775..2038)
                     /locus_tag="ACG80L_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5024408.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="ACG80L_002198"
     CDS             complement(2498..3301)
                     /locus_tag="ACG80L_002198"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5024409.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="ACG80L_002199"
     CDS             complement(3279..4403)
                     /locus_tag="ACG80L_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024410.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="ACG80L_002200"
     CDS             complement(4419..5234)
                     /locus_tag="ACG80L_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5024411.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6096)
                     /locus_tag="ACG80L_002201"
     CDS             complement(5224..6096)
                     /locus_tag="ACG80L_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5024412.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="ACG80L_002202"
     CDS             complement(6181..7470)
                     /locus_tag="ACG80L_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5024413.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7614..8126)
                     /locus_tag="ACG80L_002203"
     CDS             complement(7614..8126)
                     /locus_tag="ACG80L_002203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024414.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8193..8336)
                     /locus_tag="ACG80L_002204"
     CDS             complement(8193..8336)
                     /locus_tag="ACG80L_002204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5024415.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2742 a         1883 c         1410 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DAZUMB010000107         8836 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000107 DAZUMB010000000
VERSION     DAZUMB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.109"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(315..398)
                     /locus_tag="ACG80L_002205"
     tRNA            complement(315..398)
                     /locus_tag="ACG80L_002205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="ACG80L_002206"
     CDS             926..1426
                     /locus_tag="ACG80L_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5024416.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="ACG80L_002207"
     CDS             complement(1616..1960)
                     /locus_tag="ACG80L_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS5024417.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="ACG80L_002208"
     CDS             complement(2114..3034)
                     /locus_tag="ACG80L_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5024418.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="ACG80L_002209"
     CDS             3221..3712
                     /locus_tag="ACG80L_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS5024419.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="ACG80L_002210"
     CDS             complement(3736..6111)
                     /locus_tag="ACG80L_002210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS5024420.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="ACG80L_002211"
     CDS             6437..7063
                     /locus_tag="ACG80L_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5024421.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="ACG80L_002212"
     CDS             7086..7790
                     /locus_tag="ACG80L_002212"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5024422.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7982..8815
                     /locus_tag="ACG80L_002213"
     CDS             7982..8815
                     /locus_tag="ACG80L_002213"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS5024423.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2678 a         1682 c         1559 g         2917 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt
     7861 tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt
     7921 gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt
     7981 tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga
     8041 atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga
     8101 tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc
     8161 tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga
     8221 aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat
     8281 cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc
     8341 cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa
     8401 aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt
     8461 attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc
     8521 tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc
     8581 tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt
     8641 caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt
     8701 cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg
     8761 gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa
     8821 accagaacac ttctct
//
LOCUS       DAZUMB010000108         8645 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000108 DAZUMB010000000
VERSION     DAZUMB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.111"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG80L_002214"
     CDS             <1..135
                     /locus_tag="ACG80L_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5024424.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(88..300)
                     /locus_tag="ACG80L_002215"
     CDS             complement(88..300)
                     /locus_tag="ACG80L_002215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5024425.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFLMILFVKLSETKLSIYVTQKNF"
     gene            complement(744..1865)
                     /locus_tag="ACG80L_002216"
     CDS             complement(744..1865)
                     /locus_tag="ACG80L_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS5024426.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2193..3602
                     /gene="istA"
                     /locus_tag="ACG80L_002217"
     CDS             2193..3602
                     /gene="istA"
                     /locus_tag="ACG80L_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS5024427.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3599..4327
                     /gene="istB"
                     /locus_tag="ACG80L_002218"
     CDS             3599..4327
                     /gene="istB"
                     /locus_tag="ACG80L_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS5024428.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4455..5366)
                     /locus_tag="ACG80L_002219"
     CDS             complement(4455..5366)
                     /locus_tag="ACG80L_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5024429.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5383..6390)
                     /locus_tag="ACG80L_002220"
     CDS             complement(5383..6390)
                     /locus_tag="ACG80L_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5024430.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6387..8516)
                     /locus_tag="ACG80L_002221"
     CDS             complement(6387..8516)
                     /locus_tag="ACG80L_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5024431.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8521..8631)
                     /locus_tag="ACG80L_002222"
     CDS             complement(8521..8631)
                     /locus_tag="ACG80L_002222"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024432.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2654 a         1759 c         1505 g         2727 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat cattaaaaag ttagtaaata attgccctct
      181 aagttccacg gtctgttcat taataacttt atatcctcgt ttttcaaaaa atggctttgc
      241 ggtaatggaa gcgtgggtag tgattttttg cataccagtt tctaaaacat ggttttccaa
      301 ttcatctgca attttagtcg caataccttt tccttgatgg ctatgttttt gtcacaactt
      361 cagtattcct gtaacaatac ggatttcagt atacaacaac agcttttgaa aagatacttg
      421 caaaaggatt aagatatcta tgacctgcta tgctcactga tgttgaaaag aatgttttat
      481 taggattgag gtaagcaaga tagtgaatat gtccggaaag cctgtaaaat aaggcttttc
      541 ggacatatgt gaagttataa aaagatagcg aaaaatctat ttagtatcac taatgttgcg
      601 aacatatcgt cagaatagtc tgacatttat ttcttgctaa aaatttcaaa aaagtcaagt
      661 gctgacgcag cggatgacta ttttccagaa aattttagta tgtattcaaa aatcaaacgt
      721 gacaacagct attaactaca tcctcatcca ggtctgtaac gtttgaacca ctcttctgcg
      781 ggctcaaaca ttaagtaatg gaaggtacgt ttcaattgaa acagcgtcgc tgtcgtattc
      841 aactcaattt ttatcacata agtgagaagc gttgcgatta aggtgaggat gacttgattt
      901 atcgcccctt gttcactatg tgaatatagt cgtttaatgg tcagattttg ttttaagtgt
      961 ttgaacaaca attcaatctg ccaacgggct tgatacatgt cggccacttc gtttggcgat
     1021 acatcaaaga gattggtcac cattcgcagg attttttgtc ccttggtaag aaccgttacc
     1081 aacctaaaac gctctgtcac atggttgaca ccgccgacaa gaatcacttg ttggtcactg
     1141 attatcgtcc catcacgggt tgttgtatct gctacttcaa tttgatccaa gatggtaatt
     1201 tttgtattag attttgtccg tgtaacgaag aaatagccgt cacgatttag tttgtctaac
     1261 aacttgtaat caaagtagcc gcgatccacc acatacgttg cttgcgtttt atcaacaaga
     1321 acttcaaggt gattacggtc atgctcactc gcatttgtca tcgtaaaact ttcaggatac
     1381 tgattggttt tatcaagata acagagattc aaatgtagct tgatgccaga ctttgttttc
     1441 cgaaacttgg cccatggaaa ccatgctttg ttcaacggaa tagtcgttga atcaatcaat
     1501 tgtagactgg ttgtttttga gcttggctgc tgagcagaaa ttctggcgac taattgcgta
     1561 aaaatttcca tcaatacttc ttgcttgatt tctggcgctc tacgagataa cgagctgtaa
     1621 cacagggaat caatgccaag ttccttgcag aggcgctgat ctaaaaaggc gcgatcaatc
     1681 tcgcgatagc ttggcaattc ttcataaacc gcatgaagta acactttaag cgtcgtttgg
     1741 aagtctagtt ttttactgta gaaatcaaag tcgctaatta agctttgaga gttttctgat
     1801 aaaccagaaa aatttattgc agaaaaccat ttttgaaatg aggtttttac gttatactta
     1861 tccatgcgtg agtccttttt attggtcttg gataagtcat ccgagaatag tataaaggac
     1921 ttttttgcgt ttggatagct ttgtattagg cttttgatgg gttaaaaagt ctttgcaaca
     1981 ctagtaattt agtatttatg tataaattga aagatatttt accaccactt agtcttctta
     2041 aaagtgatcc aacattttgt ttgtggcaga atttaactac acactaatgc agccgaaagt
     2101 acacaactat gcagcagttt gactgcgaac tggtttcctt ccatcataag tttgaatgac
     2161 ttagtgatgg aggtacagaa aggttgttaa ctttggatga tattaaaaca ataaaacatt
     2221 tgagaaataa tcgtgatcag tcaattgatc agataagaaa agcaatgaat atcaattggc
     2281 gaacagcgaa aaaatacgcc gatagtgatc aactattcat tacagatccc actcgaaaaa
     2341 acaaatccat gatggatgat ggttggggag aaatcatcgg tagttggcta gaagaagacc
     2401 tacgtttacg cagaaaactt cgtagaacgg ccaaagctat ccatgaacaa ttgaaacctt
     2461 tgggtttccc tggctcctat cgaacagttt gtgtatacat ttctcaatgg aaaaagcagc
     2521 gttacgacga aacgaattac caagctgtcg atcgtcttga tcatccgatc ggagaagcac
     2581 aggtcgattt tggaacaatg gaagttatgc atgaagatca catgaaagat atacaacttc
     2641 ttatcatgtc atttccctat agtaatgcga cgtccgcaag tgccatgcca gctcaaaacc
     2701 aagagtgttt cctggaagca ttaaaacaac tatttaatca atgcggcggc gttccaaaaa
     2761 cgatcagaat agataactta actccagcgg tgaaaaaaac aaaatcaaaa tttgaagaag
     2821 cacaactaac cgatgaattc ttacgtttca ccattcatta tggttgtgat gttcaagtct
     2881 gtaacccacg tagtgggcat gaaaaaggac atgtcgaaaa taaagtgggt tatgtccgat
     2941 acaatttctt ctcgacttcg ccaatcatgg actcttttga gtccctaaat aagcaacttg
     3001 aagaacaatt aaaagcagat cgaaaaagga cgcattattc aaaacatcaa ttaatcgaag
     3061 atttgtggaa gaaagaaaat aaagcgttac attctcttcc acaagaggat tacccaattt
     3121 ttaaagaagt ggaagctaaa ctaaatcgtt atcacgaaat gaaattggat caaacggtta
     3181 ttcatattcc taaagcatat ggctattctc aattgactgt tgttttgact tggaataaat
     3241 tcaaagttct tacgcctcat ggagaaatat taatgaacga atatcgcccc tatatgaaca
     3301 aaaaaagatt gatcccatgg agtgacatca tgaaacaatg gagtaataaa ttgaatgtcg
     3361 ttagttattc gaggtattgg aagtacttgc ctgcacgcct tcaaaattat ctagcgattg
     3421 ataataatca cctgaaatac agacggataa aacaattact aagtttatta gcgacctacg
     3481 atatccaaca aattaatgaa caattttacg aattagtcgt tcaagaagac gccccctctg
     3541 actttgatgt gaattggtca aactatgatg cattaacaaa aacaggaggt gtgcaaaaat
     3601 gattggacag acgttagaag attattgtaa aaagcttcga ttaagccata tcatcacgaa
     3661 cataccaaga gcttctttcg aagctccaga acaatttatt accgatttat tccgtttaga
     3721 actggagacc agacagcaga tcaaattaga acggatggaa aaattagcca aatttcctgt
     3781 accaaaatcg ctaaagaact atgaatggca cgaagatatt tttctaccat ctcatctaga
     3841 taaggagacc cttgtctctc ttgattttct aaaaaggcaa gaaaacattg tttgtgttgg
     3901 ctctcctggt acagggaaaa cacatctagc gatcgcatta gggagaaaag cctgtagtga
     3961 aggtgtttca accaaatttt tccgagttaa caatctcgta gagcaattag agaatgcatt
     4021 aaaaaaacaa cagctagcca gtttcaagaa gcgattcgaa acaacagaat taattattct
     4081 tgatgaaatg gggtacttac cattgacaaa agaaggagct gaacttctct tccaactgat
     4141 ttcagattgg tatgagcaaa agagcattat tattacctct aatttagaat tcagtcaatg
     4201 gaatcgagtg tttatggatt ctcgattaac agctgctctc gttgatcggc taatccatca
     4261 cgctcacatt atcagcttca aaggcgaaag tttcagattg aaaaattcat tatctaaatc
     4321 atattgaagg aaaaaactag ttgcagcaaa actctgcact ttcggctgca tttttttgta
     4381 aaaagctatt gcacaacaca aacatttgaa cgacgtaatt ccaaatgttg gattgctttt
     4441 agataatctt ttatttattc acaattgcta atagtccaat tttcattttt gtatagtttt
     4501 aatctatact gaaaaacact tgttgttttg gacaactcat caagatactc aacaccaaca
     4561 atcacttgaa tcgctttacc cttttgtttg taaataggat tcactaattc cacaaatttc
     4621 aaattagtat taattggttg catcgcgtct ttttcaacat aatgctctag ttctttttct
     4681 gaggctgctg gataaagcct gaaaaacgtc tctaaaaact cggtgatttc ttccgttgtc
     4741 tttgaatcaa tttttgaatc actttttact tgtcttactt tatgttttgc cttttctggc
     4801 ttaccaatca tagtgggtaa actcgttaca atatgattgc ctttttcatc ttcaaagatc
     4861 atcagacgat atgcagaaga aattgattgt gtctttttcc cttctgtaat cttttgatct
     4921 accacaaatg aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttactgat
     4981 acaacctcgg aactagtggg aatatccgct cgaaccatat cttggctcaa agcgagccct
     5041 tcttctgtaa ggtactgctc aagattagtc attctctttt caattacctc cttgttattc
     5101 ttccatgaaa aatattcttt cacaaaatct acagtaaaat tttctactcc agaggtattc
     5161 actattttct ctttcacgac cactttctca tgagttgtat gctgatcaat ggccgtgaag
     5221 tttttgtaga ttccaaaagc aaggctacaa gacaataaaa gccaaagggc aagcaccatt
     5281 tttcgatgct tgcccacgga tactactcgt tctttcttct tttttggaat cttttccttg
     5341 ttacttcgct caatttttat cttcattttc cattcctctt ttctattgtt tataccgacc
     5401 agcaccaata atgtgctgtt gccaatagct atcggtaaga ttggtccaac ccaccggatc
     5461 tcctgcatga aacattctgt tctctcctgc ataaatgccc acatgagtca cataagtacc
     5521 ggcatcataa gttgaatgaa agaaaatcaa atcgcctggt ttggcatctt ttaagtcaat
     5581 atgagtcata gcatcatatt gatcctgagc cacccgaggg agtttcaaac caactttggc
     5641 ataacaccat tgagttaaac cactacaatc aaacccagtt ccgggacttg acccaccaaa
     5701 tacataagcc gttccttcat acttgaacgc ctcgtcaaaa attccttgga ctgttttatc
     5761 atcaaactgt gttgtagtta ggtactgctt cactaataag caataaaaca tattgccata
     5821 gttataccgc cacccactat tttccttaat cgcgatggga ttgggataat cgactttttg
     5881 accacccgat ttttcttttg aaaaagattc ggctaaagca aaactatgct tctttccgtt
     5941 tttcgcgaca taatccaaat acccgtcacc atagttataa gcctgaatga tactatcttg
     6001 atcgcatcct agttctttgg aacacttaac caaagacgaa aaatagagac acacttgttt
     6061 gatggatgcc tcggtactta atgaattcgg tgataaacct gctgattcag aactttgcat
     6121 gacatcttcc attgtacctc cagattcgac ttgaataatc gccaaaagaa tcgaaacata
     6181 ctctgttata tctgattctt tggcatactt ttccaccatt gaacggtgtt tcaaaactac
     6241 ttctgagaca ctactacctc caggagaagt tccagaacta ccacttccct caccgtcctc
     6301 accaaacaac aaacccacac acatcaagat gcccacaaaa gagatggtca ggaaggttaa
     6361 cgaaataata ctcaattttt ttaagttcat cttttctcac ctggctttgg ttttgggcga
     6421 gtgttctttt ttctttgtac ttgttgattg gttcgctttg gaaaagtgaa tctctgtttc
     6481 acttgttgaa aggaattgtt cttgcttagt ggctgagtgc ttccgtttgg aatgacttct
     6541 tgactagaaa gtttccgttt gatttcagga ttcgaatgta cttgtaccct tggttttaaa
     6601 gacctatttt ctggtagttc atttttctgt ggttttatct ccttttttat cggaacattt
     6661 tcccttcctt tctcatgacg attccatgct tcgttcaata ctttgcgttt ttcatccatt
     6721 tttgcgtctc ttcgttgtcg ttgtttttct cgcaactccc ctcgatttgc tttttcttcg
     6781 aaaggccctc gcttaaagtc ctctggtact tttttcgtct tttccatgcc tttatgaagg
     6841 ctgtatttca agttagttgg tgcatcggtt acttgatttt ttgcttgttt cgctttgtcc
     6901 ttcacgattt cttttgtatc gagtactttt ccaacttttc taccaactaa atttattcgt
     6961 gattctttct tcgtgcccac tggttgttct tgttttttca gatcagaatt tagctcgtta
     7021 atgcttctcg cggaatggtt ctttctattg gacactctat tgatttttga actacctttc
     7081 ccttttgtag aactggtagc tagtttccca ccaactgctg caccgccacc aaaagcaaaa
     7141 gttcgaccca catttcgatt aagctgtcgc aattttcgat tcatcaacat ttgcggtttc
     7201 cgcatgactc gccttcccat tgactgacta tcattacttt gaaggttaaa catgctcata
     7261 atgtcaccta atttgaaata gataccggca aaggtcacaa tttgtagaaa agctaccata
     7321 aagaagggat aggtggccga aatattgaaa aacatcgtgg agatactgaa tgcagtcgta
     7381 atgactaagg taactcccgc tcgcatcata atcgtattga acaaacgaat aatcgccctt
     7441 ttgcccaaac tctcatacgt cggcaacatc gacaataaga aactaatggg cagaaacatc
     7501 gcaaagataa tgaaaaggat ttgtgagaac aacataatcc ctgtcagaag aaacacaaaa
     7561 aaggaaatcc caatattaaa caacactaaa aagaccacgg ttcctaaacg gttcatggtc
     7621 ttggtaatac tgagattcgc attgtcatta tcttcaatct ctgctttgac tgcttcttcc
     7681 cgatccttac ccttattttc atccggactc acggataaaa tcttattaac ccgaccctcg
     7741 ccaatttctt ctatattgga atcatcgaat tgtaatagta accagggttg ttgaacttga
     7801 atcgaaaata aactgtctcg aatcaaatcc acactatctt ttccctggct ttctgaatta
     7861 ggtacaacaa tcttggttcc aaggctaaga gccgcttcac taacgtctga gctaaagtca
     7921 ttgatttttg taatataggt gggcgcatac gcaatgaaag aacccgagag tagaaaaatc
     7981 atcacaaaat taatgacggt tcggactgct ttggtagttt ctcgttttaa taatccggta
     8041 tacgccacat aaactcctag agctaaaatc atcaataata ggaacccaaa gtagaatccc
     8101 gaagtctgta atccattttc agtgataccg gctaaagttt ggatattctt tccaatactt
     8161 ttagccgtat ccgaaatgaa atccaacttg taagcttctt gaaccacata gcccgtggcg
     8221 tttgataaat acagacttac tgtccaaata aaattggtaa tggcataaag accatacatc
     8281 acgcttttcc ctaagccatc cccccaattc caaggtaacc aatcccaaga actatccaca
     8341 aagaaatcca gttgataatt gttcagagaa tatttggaat aaaggtttcc ggcttcaact
     8401 gtatcatcaa ccagacctgt cgcctcagct accgtaccga ctaaactcaa tagaactgtc
     8461 agagtgacga tgctaataaa aacgattccc acaatccgaa aaagtttctt tttcatcccc
     8521 tcactccatt ctctcttgaa ccggcggacg agtgtcaaaa gcatggaaga gttcttcaaa
     8581 gactggatga acttgaacca cacctactcg cccatacaag tcttgcatca agcattgacc
     8641 gtttt
//
LOCUS       DAZUMB010000109         8465 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000109 DAZUMB010000000
VERSION     DAZUMB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.112"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG80L_002223"
     CDS             476..1339
                     /locus_tag="ACG80L_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5024433.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG80L_002224"
     CDS             complement(1468..3090)
                     /locus_tag="ACG80L_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5024434.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG80L_002225"
     CDS             3339..3860
                     /locus_tag="ACG80L_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5024435.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG80L_002226"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG80L_002226"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS5024436.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG80L_002227"
     CDS             5941..6759
                     /locus_tag="ACG80L_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS5024437.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG80L_002228"
     CDS             complement(6862..7059)
                     /locus_tag="ACG80L_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024438.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG80L_002229"
     CDS             7228..7806
                     /locus_tag="ACG80L_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5024439.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG80L_002230"
     CDS             7901..8038
                     /locus_tag="ACG80L_002230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024440.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2558 a         1515 c         1655 g         2737 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatca
//
LOCUS       DAZUMB010000110         8325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000110 DAZUMB010000000
VERSION     DAZUMB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.113"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(180..1286)
                     /locus_tag="ACG80L_002231"
     CDS             complement(180..1286)
                     /locus_tag="ACG80L_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS5024441.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1286..2422)
                     /locus_tag="ACG80L_002232"
     CDS             complement(1286..2422)
                     /locus_tag="ACG80L_002232"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5024442.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(2454..3905)
                     /locus_tag="ACG80L_002233"
     CDS             complement(2454..3905)
                     /locus_tag="ACG80L_002233"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5024443.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3949..4713)
                     /locus_tag="ACG80L_002234"
     CDS             complement(3949..4713)
                     /locus_tag="ACG80L_002234"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS5024444.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4741..5439)
                     /locus_tag="ACG80L_002235"
     CDS             complement(4741..5439)
                     /locus_tag="ACG80L_002235"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS5024445.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5451..6230)
                     /locus_tag="ACG80L_002236"
     CDS             complement(5451..6230)
                     /locus_tag="ACG80L_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS5024446.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6246..7190)
                     /locus_tag="ACG80L_002237"
     CDS             complement(6246..7190)
                     /locus_tag="ACG80L_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5024447.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7236..8015)
                     /locus_tag="ACG80L_002238"
     CDS             complement(7236..8015)
                     /locus_tag="ACG80L_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024448.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2363 a         1736 c         1249 g         2977 t
ORIGIN      
        1 ctgtttttta ctttcattat atccattacg attcatgact tgaatatcaa ttttagcttg
       61 taacgtggca aatgcttcct tccaagatgt tatctttgca attcgtcttg ttgcattcaa
      121 ttcttcggat atgcgatgta taattctttt tatacttgaa tgtttctgtg tcatattctt
      181 taacctcttt gtattaattc aaataactca taatattttg attctaagat tttgtatgct
      241 gtttgaatat tataatctgc ctttattaaa atcgaacgtt ctgaatctcg atgagagata
      301 tctacatttg caatctctct cgcccattct tttgccgatt gatttaaact tatgaaattg
      361 gtcttatcac tgaaagcaac ttctgtagga actttctccg aaaagaaaca cggtaaatct
      421 gcaaattgag cttcaatacc cactaacgga accccttcaa ataatgatgg caacattaat
      481 acgtcaaatg cttgataaaa tttttctata tcatttctat ttcctaaaaa tttaatatca
      541 tcatctatgc cacttttctt tgcataattt tttatttctt cttccatatc accagttcca
      601 ataattaata atacggaatt attcttaata gcgtgaaatt ctttaaaaat ttcaatcaaa
      661 aaacgatgat ttttagaata tgaaattctt ccaatatgtc ccattacaaa cgtctgactt
      721 gaaattccta atttcattcg tacatcattt ctaatacttt catcataata aaatttttta
      781 gcatccactg catttggaat caaagtgaat ttatcatttc gaaacaaaaa atcacctgct
      841 acttttccac atgaaaattc atgtgtcaaa acagacgtta acttacatcg ataatattgc
      901 tttaaaagat atttaaaatc tctatctatc gatgtattat ggctatgtgc tatccgtatc
      961 ggaatcccag ctttttttgc agcttttaaa ggtaaataac tcatcgcatc aatatgagaa
     1021 tgaacaatct catattctgg atgatcctta aaaaattgtt ccatccattt aaaatattta
     1081 ggataatttt gtggataaag tctcggtgca taatagacct ttcctccaag acttattatt
     1141 tcctcatcat aatcactttt atatggacga tgtgtcaaaa aatcaaactg aattttagtt
     1201 cgatctatat tcctataata gttcattagc atcgtctcca accctgcacg atgcatatca
     1261 ttcacacact gtaatattcg aatcattata tatttcccct atacaaattt aatgtttctt
     1321 gtacgatttt atcccaatta tatttactac aaataaactc agtagcttga ttcttcaaaa
     1381 ctttaaccat ttccgattga ttgcatgcct cttctaatct tatctttaaa tcacttacat
     1441 ccgatttctt aaaaatcata gccttgtctt ctactacttc cgtacactct gagatatttg
     1501 aaactataca acagttccca taactcattg cttctaacaa acttaatggc attccttcta
     1561 aatcacttgg aagtgtatag atatacgcat tactatataa ttcttctaat tcctgtcctt
     1621 gtacaaatcc agtaaatata attcgctcat caccttttgc taattccttt agttcatttg
     1681 caaattcatc tgtatcagag cttcctccgg ctattataag ttttttatct gtctgaacat
     1741 ctttaaatgc ctcaattaga taccgaattc ctttttcggg aaccaatcgc cccaaaaaca
     1801 aaatataaga atcttttgtt aatccatact tttctgtaat caatcctgct gattttattt
     1861 caattttttt aacgccattt ggtataaacc ttgtttttct tccatatata tcttcaaaat
     1921 aattttggac gctttcacta agtacaatta tttcatctgc aaattttaca gcacactttt
     1981 cccctaacat gatgtatgta ctagcaagct tgttccattt ttctctttga tgatctagac
     2041 catgaattgt tgcaacacat cttttcccaa aaagttttgg caaccacaac atagcacatg
     2101 gtccttctgc atgaaaatga acaacatcat attttccgaa tgctgctcta attccaccaa
     2161 atactgaaga actcattgct gcaattcctt ttatatttaa tgtaaaaata gtctttaatt
     2221 ttacaccttt atattcttta tttttatgct ggtcaaattc ctttccgcta acatgatgac
     2281 cacttcgatt atagcaagtc acttcatgcc caagtgcaac cattcgcgtt gccaattctt
     2341 ctaccactat ctcaattcca ccttctcttg atggaattct tttatgacct agcatacaga
     2401 tttttaattt ttttcttgcc atcacgtttt actccttcta tagtatcttt atcttacatg
     2461 gaaccgtttc tatgaagcac agttttgact gtcctaaaca aaattctaaa atctaatccc
     2521 atactccaat tcctgatata ttctgcatca agttttacaa cctcttcgaa gtctgtgata
     2581 tcacttctac cactcacctg ccacattcca gtaatcccag gtttcatcgc aattctcgca
     2641 cgatgatgaa aatcatactt ctcatattct gacttcaagc aaggtctaaa ccccaccagg
     2701 ctcatttttc ccattaggac attataaaat tgtggaaact catccaatga tgtagatcga
     2761 ataaattgtc caattcctgt cttctttgtt ccatcaggaa gtattttatt gccaatgatt
     2821 cttgggtcaa aatccatctt aaacatgaga ttattactca tcttattttc tttcatcaac
     2881 tctgctttgc gttcttctgc atccatatac atactacgga acttatacat cttaaatgtc
     2941 ttaccatttt gaccaatacg ttcttgtgca aagaaaatcg gccctggtga tttgatgtaa
     3001 ataactggtg ctacgaagat aaaaataaca cctgttgcaa tacatccaat aattccagca
     3061 agaatgtcaa tcgttctctt tataaatgct tgcctcatcg tcataacatt aatactagat
     3121 gtaataacag tataatcacc aactcggcca ataaattgtt ttccacgtgt actatcagac
     3181 acttttgtaa gattcatatg tacagcaagt cccatttccg aacatctggc gattaattct
     3241 ttgggatatg gaacactttc gtcaacattg atgaatactt catctaccca attctgtaaa
     3301 agatattccg ctgcattatc agcatttgca actactggga cacctccgat ggtagttcca
     3361 tacatatctc tgtctactac aatcacacca ctaagtttat acatgccata attcttctct
     3421 ttaagattac gaactacatt ttttgcaatc ttttgagatg ttacaataac taacgataca
     3481 tcttcaccgt ttttcatgcg gtgatgcaac aaccctttcc aaagtatacg aattatatat
     3541 gttaataatc catacaccac ccccattata aataaagaaa ctctggaata atcatttcca
     3601 tcctgcattg taataaggta taaactgcac acaagtaaca gcataaaaga ttgctttata
     3661 gaagctacag cctctttata atatcctctt tttaaaacat ttttatatgc ctcaaataaa
     3721 aatataatcg taagattctc aagttctata aaaattgcca tgtggcgata aataggaatc
     3781 acatacggat tccagtcacc ctgtcgcatt acatatgata tataaaaagc aagctggaca
     3841 caaaataagt ctaataaaat aaaatcaata tgtttagacc aaccacttga gttttttcta
     3901 tacatctctc actctctcct catttccttt ttatctacga ataaagaact aaaataaacc
     3961 aaacttcttc ttttttacct cttcatagat tggataattg atctcatccc cgttgatcag
     4021 atcctttgcg acttgtttca tctgtactac tttttcctta ccaaactcct tggcaatctg
     4081 ctcatagcac tcttttaagt aaaaggtgcg cttagagacc ccgtgtgcat cagaagcagc
     4141 catctgcacg agattatgtt tgaccattgt tttggctgtt ttctggatat ctttgccaaa
     4201 tacaccaaca taacttggcg cagtaagttg ggccaaacat cccatatcta ggaacggaag
     4261 caaatgattt gggtcttctc gaaaatgcgc gttgcgttct ggatggacga tcactggtat
     4321 ctttcctagt tctaacaact caaaaaaaag ccgttctgca tataacggca cttccagtgt
     4381 cggaaattcg atcagaagat atgtatcatc taaatctgta aataaaattt catctcggtg
     4441 gatgtcttcg atgagcgttc cggtaatacg tacttcttgt ccttccaata acgtcaaagg
     4501 caactgtctt ttctctaatt cggcttgtaa cgaagcgact agagaaatga cttgtgattt
     4561 ttcattgctg tattttccat tattgtggtg tggtgtgcat aaaatatgcg taatgccttg
     4621 ctgaatcgct ttttcagcca ttgcaatact tgcttctaag ttttctgccc cgtcatcgat
     4681 tcctggtagg atatggcaat gtagatcgat catttctctt gtctcctctc ttcttattcc
     4741 ttaatttccg tagtaataac cagcatcttt gctgtgttct gctccgttgt atacgacacc
     4801 taagactcgt gcttgtacca tatctaacag ttcttttgct ttagtcaatg attcttttcg
     4861 tgacgtattt tcccgtacga ccaataaggt gccatccgtt tttgaagaca tgatctgtgc
     4921 atctgttacg gcaacgactg gcggcatgtc gaagatcact acatcaaata gttgtcttgc
     4981 ttctgctaaa atctgatcca ttcttggcga acttagtagt tctgatggat ttggtggttt
     5041 aggtccactt ggcagaatcg acaaattgtc gactggtgtt cgttggatct catctgctac
     5101 acttcctgat gaactaagaa cagtacttaa tccgcttgcg ttgttcaact tgaacgtttt
     5161 atagatgacg ggcttacgta agtctgcatc gactagcagc acccgttgac cagatttggc
     5221 aaaaacaacg gcaatgtttg ctgcagtggt cgatttccct tctccaggtc ctgatgaagt
     5281 cacaacaatc gttttgattt gctgaccagc tgcggaggca aactggatat tcgtccgaat
     5341 cgtacgatat tgttcagcga ttggtgaaga gggattggtg agtgtgacca aactgactgc
     5401 atgagtttgg gctgcttttt gtttctgtgt tcgtgccatt ttctttcctc ctagactctt
     5461 gagcggcttc tgcgtgatgg cgctgtttta tcgtttgtag cttttggttg tgtctgagct
     5521 ggtccttttt tacggatacc agcattcaat tctttcgctg acatttctgg aactgttcct
     5581 aagattggga atcctaatgt atctgtcaca tatttttcat ctttcactgt ccgatcgagt
     5641 aattccaata agaatgccag acctacacct accatcattc ctaacattaa gccagtcatt
     5701 aaattcaatt tattatttgg cgaaacaggt gtcgtattgg caactgcacc tgaaatgatc
     5761 gagattttat caacattcat cacatctttt gcgttctctt ggaaaacttg tgaagttgtg
     5821 ttagcgatct gttgagcagc cactgcattc gtacttattg cctgaatcga gaacatttgt
     5881 gaattttctg cctgattaac tgagatggca tctttgattt gttcagcagt cattttcaca
     5941 ttgtcttctg tttctaaacg atctttgact tcattgagca ctaaatcact gacgatcata
     6001 tctttatacg tattgatcat ttgcaggttc gtattgacat cattgacatt tgcagtgtct
     6061 gattgtggca gtgtcacgat caattgtgct tgtgaactgt attttggtgt caagatgaaa
     6121 aatgttacga ttcctgagat ggccaagcca gcaaacattg agatgatgat tattaccaag
     6181 tgtttgcgta atgtttcaaa aatttctttc aagctgattg tctcttccat tttttctcca
     6241 ctttcctatt tattctctag attcatttga tcttttaatt cttttttcac acgatcaagt
     6301 tcttgatcac tgactttttg ataggagata ttatcttgca tgaagccttc tccttgcagc
     6361 tgatcttgct taatcgtacc aaatgcctcc cgataatcta aagcaatttt tttcatttca
     6421 tcaaagttca tgtctgtttt catattcgac tctagagcat tcaaaacgga ttgatagttg
     6481 ctgacaccgt ttagactcaa tacttttttc gctacgcctt ctacgatctt tctttggcgc
     6541 ccttgtctac cgtaatcacc atttgggtct tcataacgca tacgagaata agataatgcc
     6601 tgatccccgt tcaatgtaat tttaccgatt ggaaagtcat agccatcttg tgagaaagtg
     6661 aggtcattat tgacttcgat accaccaact gcatcaacga gttcctttag ccctttcata
     6721 ttgatagaga catagtgatc gattgggatg tctacatatt tttgtacggt atccatcgcc
     6781 atggcagctc cgccgaatgc gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg
     6841 atgtcgacat acgtatcacg aggaatgctg acgatcgttg tctgtttgtc ttcaggactg
     6901 actgtggcaa ccatcatcgt atctgatctt ccttgatcga cccgacctaa atctccagta
     6961 tcaatcccca tcaatagaac agagaaagat tcttgtttgt ccagatcaac tggtttcgtt
     7021 cgttctcctt gggttcgttc cactgattcg taagttttct caatagaatg tgagagatcc
     7081 agatataatt ttgctcctac tcctacaatt gctaatacta atacgataag aactgctaaa
     7141 ataatcagaa atattttttt ccctcttgat tttcttcttc gtcttgccat agtttcctcc
     7201 gtttgctaac cttgcttgaa tcaacgtttt aactgtcaat agcttatatt gctatagccc
     7261 atttgctcag caacagtttg tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg
     7321 atctgctcta tttgactgag caccgtcaaa tattgatcgt aagtagcggt ggcagtcact
     7381 tgaccatcct ttaacatcgt atcgaataac tgatctagtt gcgtgacttg cgcgatctga
     7441 atatcagcga atcctagata ttgcataaca accgttttcc aaggactatc tttaaaaaag
     7501 ctcatatttt cacgtacacg agcgacatct gcacttgtca ccttttcttt gatgatgaca
     7561 tcgtctacag cttgctgcca gtttgataca tttttagtaa ataaatcgtt gacagctgtc
     7621 tggatcttca gttttgattc tgcactatct aactgtgtca cgattgcttt cttctcatct
     7681 tggattgcaa cagcttcttt aggcaattct gattctttga tgtcgtaatc atctgctgaa
     7741 agtttgacag aggatagctt atttgacaaa cttgtgattg tttcttctgt tatttcaggg
     7801 tttagaaaaa ttttttcttt tgtactatac agcttgtcta cttcttttga taaagaagtc
     7861 aaggtgtctt tttgattggt gatggcagat tctgcataat tgatccgttg ctgatgttgc
     7921 cgctgattat tgatatggta tccaaacaaa cagatcgcaa tagcacctgc acctaccgcc
     7981 acagcagtca atgacttgac gaatggcttt tccatagatt cactcctaag tattgatgat
     8041 agataaatta agatataatt cacttacata aaaatgacag ctttttattt aaaaaaacat
     8101 aattaaacta atatattcac agaaatattt gtaaacaagt tttccctata taatcatacc
     8161 acaaactgtt ttcttgacta gggcaattta cgatttaaaa gtaaataaaa cattaataag
     8221 ataaaaatta atcgatttca tttttatttc cataattttc ataggtgttt ttaaaagtta
     8281 cacaaaaaat agaataacaa aaaaattaac attgaattaa taata
//
LOCUS       DAZUMB010000111         8245 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000111 DAZUMB010000000
VERSION     DAZUMB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.114"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(223..1005)
                     /locus_tag="ACG80L_002239"
     CDS             complement(223..1005)
                     /locus_tag="ACG80L_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024449.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1070..1906)
                     /locus_tag="ACG80L_002240"
     CDS             complement(1070..1906)
                     /locus_tag="ACG80L_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5024450.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1924..3333)
                     /locus_tag="ACG80L_002241"
     CDS             complement(1924..3333)
                     /locus_tag="ACG80L_002241"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5024451.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3347..5170)
                     /locus_tag="ACG80L_002242"
     CDS             complement(3347..5170)
                     /locus_tag="ACG80L_002242"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5024452.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5450..5677)
                     /locus_tag="ACG80L_002243"
     CDS             complement(5450..5677)
                     /locus_tag="ACG80L_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024453.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(6013..6339)
                     /locus_tag="ACG80L_002244"
     CDS             complement(6013..6339)
                     /locus_tag="ACG80L_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024454.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6385..6747)
                     /locus_tag="ACG80L_002245"
     CDS             complement(6385..6747)
                     /locus_tag="ACG80L_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS5024455.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6784..6999)
                     /locus_tag="ACG80L_002246"
     CDS             complement(6784..6999)
                     /locus_tag="ACG80L_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5024456.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7126..7461
                     /locus_tag="ACG80L_002247"
     CDS             7126..7461
                     /locus_tag="ACG80L_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5024457.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7537..>8245)
                     /locus_tag="ACG80L_002248"
     CDS             complement(7537..>8245)
                     /locus_tag="ACG80L_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024458.1"
                     /translation="TFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFNHKRSNVYRL
                     DVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSK
                     EKQLILDEAHEVLQGLSWQGTYLMTEKELINTVCERLRNVGDNSVKNALIKNRGYFVK
                     QGFTLERASKDIKKLYNVKASSSFLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVT
                     RDDGRRVRKRKDPIVLN"
BASE COUNT         2530 a         1753 c         1274 g         2688 t
ORIGIN      
        1 aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt
       61 aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga
      121 aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata
      181 acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt
      241 aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt
      301 cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact
      361 gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt
      421 atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc
      481 tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc
      541 tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc
      601 tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt
      661 agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc
      721 aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag
      781 cattttgttg tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg
      841 gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc
      901 tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt
      961 agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt
     1021 agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc
     1081 ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt
     1141 cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca
     1201 ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa
     1261 taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt
     1321 tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca
     1381 tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc
     1441 cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc
     1501 agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa
     1561 aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa
     1621 atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga
     1681 cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag
     1741 catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc
     1801 ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat
     1861 ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc
     1921 tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt
     1981 tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg
     2041 cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa
     2101 aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc
     2161 aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc
     2221 attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg
     2281 tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc
     2341 tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac
     2401 atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat
     2461 aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa
     2521 tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc
     2581 taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat cttctggact
     2641 ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt
     2701 caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg
     2761 aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg
     2821 aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc
     2881 agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc
     2941 gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat
     3001 tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt
     3061 tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa
     3121 gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac
     3181 ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc
     3241 atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt
     3301 ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca
     3361 tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac
     3421 tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat
     3481 cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa
     3541 agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt
     3601 ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa
     3661 caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct
     3721 tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt
     3781 caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc
     3841 caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt
     3901 gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa
     3961 caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa
     4021 tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag
     4081 taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt
     4141 tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc
     4201 ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca
     4261 ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa
     4321 ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca
     4381 acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca
     4441 tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat
     4501 aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta
     4561 ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg
     4621 gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg
     4681 ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca
     4741 aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa
     4801 tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa
     4861 tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa
     4921 ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca
     4981 ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg
     5041 ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg
     5101 ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc
     5161 tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca
     5221 atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata
     5281 acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt
     5341 aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta
     5401 gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta
     5461 aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat
     5521 ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag
     5581 taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt
     5641 tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcgata
     5701 gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc
     5761 taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat
     5821 ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa
     5881 attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa tttataggaa
     5941 actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat
     6001 aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg
     6061 gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc
     6121 tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg
     6181 atttaagttg tactgttctt gtaaaatctg tgttaccaat ggttttgatg acttctctaa
     6241 tccagatgta cctgtcattt ttgaaaaaat aatacctata atagccaaga caacaatacc
     6301 tataatgaca aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa
     6361 aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat
     6421 tgctccagct acccctgcta tcatcaaaat cgtacccatt ttctcatctt tttctcgata
     6481 taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgatac
     6541 taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat
     6601 ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt
     6661 tgtctcatct gtttcttgta gtgctccaca aaactggcaa tacttacttt catctgttat
     6721 ttctttgcca cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat
     6781 tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa
     6841 tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga
     6901 acttacacat tgctgtgtaa cattcatttt ttttgctaac tccgtttgag aaagtccttt
     6961 ttcttttctg tatcggctga gattacttac ttttttcatg attctcccct cctgttttat
     7021 tatatacaac aatatgatgt atttcaactc ataaaatata aaaaacttca aataccatta
     7081 caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa
     7141 tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct
     7201 gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct
     7261 aacctcgata gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta
     7321 gacaatgttg tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact
     7381 gaagatgcag ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc
     7441 aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt
     7501 gagatgaaat aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt
     7561 tctcttcctt actctccgtc catcatctct tgttacactt gtaaattgat acagaggaac
     7621 tatcccagct cctgataata tcaattcttt tattgtttca tctatccctg cccctttcgc
     7681 taaatatatt aaaaaactac tacttgcttt cacattgtat agtttcttaa tatccttaga
     7741 agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgtttttta ttaaagcatt
     7801 tttcacgctg ttatccccaa cattacgtag gcgttcacaa acagtgttaa taagttcttt
     7861 ctctgtcatt agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc
     7921 taaaatcaat tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa atactctttt
     7981 agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa
     8041 atgaattttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc
     8101 attaaaaacg tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt
     8161 ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa
     8221 tttttttatc aatcctataa aagtc
//
LOCUS       DAZUMB010000112         8185 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000112 DAZUMB010000000
VERSION     DAZUMB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.115"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..305
                     /locus_tag="ACG80L_002249"
     CDS             18..305
                     /locus_tag="ACG80L_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024459.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            721..2259
                     /locus_tag="ACG80L_002250"
     CDS             721..2259
                     /locus_tag="ACG80L_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS5024460.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2256..3221
                     /locus_tag="ACG80L_002251"
     CDS             2256..3221
                     /locus_tag="ACG80L_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024461.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3208..4530
                     /locus_tag="ACG80L_002252"
     CDS             3208..4530
                     /locus_tag="ACG80L_002252"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5024462.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4593..4697
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4771..5514
                     /locus_tag="ACG80L_002253"
     CDS             4771..5514
                     /locus_tag="ACG80L_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5024463.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5529..6521
                     /locus_tag="ACG80L_002254"
     CDS             5529..6521
                     /locus_tag="ACG80L_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5024464.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6533..7270
                     /locus_tag="ACG80L_002255"
     CDS             6533..7270
                     /locus_tag="ACG80L_002255"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024465.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7260..7982
                     /locus_tag="ACG80L_002256"
     CDS             7260..7982
                     /locus_tag="ACG80L_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5024466.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8066..>8185)
                     /locus_tag="ACG80L_002257"
     CDS             complement(8066..>8185)
                     /locus_tag="ACG80L_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5024467.1"
                     /translation="YLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2687 a         1402 c         1751 g         2345 t
ORIGIN      
        1 aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca aaatcaccca
       61 aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg cctgtgacaa
      121 tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc aaagcaccac
      181 ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct gtttattatc
      241 agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat aaagtggtga
      301 tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt ttttacattt
      361 ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat aaaggtgttc
      421 tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa agttgtgtgt
      481 gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat ggtattcgta
      541 gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct ttttttgtca
      601 gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa ggcaagacaa
      661 gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa ggaaggtcgc
      721 atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga agctgcattt
      781 caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc tattccttta
      841 gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat cacgggtgtg
      901 ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt agggatcgga
      961 actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt ccaaaacacg
     1021 tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc atttggtatg
     1081 tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt cattatctca
     1141 ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc attgatcgga
     1201 gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc tgttttgaca
     1261 ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact gattcgctgg
     1321 ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt gtgtttattt
     1381 ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac gattgctgga
     1441 aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat tgaagacaac
     1501 acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt gttcaatgca
     1561 ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct agaaagcttg
     1621 gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac atatgacgaa
     1681 gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat ggcttaccaa
     1741 aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact taaaaagatt
     1801 tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga atttattgat
     1861 cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc aagtgtacaa
     1921 agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa tattgtggat
     1981 gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga agatgatcga
     2041 gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc taaagaacat
     2101 cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacggagga tcaaatgatt
     2161 gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc acgaatcttc
     2221 ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc gagttcaaac
     2281 atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg gcaatcaaaa
     2341 aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact actttgaaaa
     2401 tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga aaatcgttaa
     2461 atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag caaatcgctt
     2521 tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg ttaggttggg
     2581 aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc ttatctcctg
     2641 atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa cggatcggga
     2701 tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca tttagcgcat
     2761 tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac gaagaattgg
     2821 gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta ggtgatcgta
     2881 ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag ttattgatga
     2941 atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg aaagagtggc
     3001 gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa aatgctcgtc
     3061 aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg gtgtatccaa
     3121 gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa gaaatctttg
     3181 cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat catcggttca
     3241 ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac agtactagtg
     3301 gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc taaaaaagtc
     3361 ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa aggttttaac
     3421 gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt tacggatcca
     3481 gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct ttcaggaacc
     3541 gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca tgcggattac
     3601 ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa ggaatacttg
     3661 aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat ttttatcggt
     3721 ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag caaagtaacc
     3781 attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt agaggaagcc
     3841 gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac ccaaaagatc
     3901 atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt agcagatatg
     3961 attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga gcaagcgaat
     4021 atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag caatccaaaa
     4081 atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc agtcgccact
     4141 tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc tatcaaatat
     4201 ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg agttacaaaa
     4261 agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg gttcacctat
     4321 catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca aaattatttg
     4381 attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt gacactcgtc
     4441 atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta tccgacattg
     4501 gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg cttgttgctt
     4561 tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg tcgtagacga
     4621 catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact ggtgaaggga
     4681 gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag cgttttttgt
     4741 tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc agcagtcagt
     4801 atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat acctgccttt
     4861 attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca agtattactt
     4921 tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc tagcgttctt
     4981 tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc tttttatccg
     5041 ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact taccctatgg
     5101 tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta ttttccgatc
     5161 gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat ctttttgaaa
     5221 acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc gggtttatcc
     5281 ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc agtcgttgga
     5341 gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa aaacagctac
     5401 cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt attgctgaat
     5461 ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt ttaagagaag
     5521 gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta tccgcttgtg
     5581 gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa gtgacacttg
     5641 ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa gaaaaaggct
     5701 attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat tcgacaagta
     5761 ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa gatgtcactt
     5821 atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg atcaaacata
     5881 atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa gattttgaag
     5941 gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa gctgtgatgg
     6001 aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga gaagggccag
     6061 aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat atgatcaaag
     6121 caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat gaacggttag
     6181 attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat ccagaattgg
     6241 tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag gatccaaatg
     6301 acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt ttagaagaat
     6361 cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga ttgatggaag
     6421 aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc aagcaagatg
     6481 taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag atatgtcaat
     6541 ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag acatccattt
     6601 cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg gtaagtcaac
     6661 attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca aagtagacga
     6721 tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag atatgttact
     6781 tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc atctaacaat
     6841 caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct atgaacattt
     6901 tttgccggaa caattatctg gcgggatgaa gcagcgaacg gccttgttac gaacaatcat
     6961 gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg ctatgaccag
     7021 agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga ctactttact
     7081 tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg tgctttccgc
     7141 ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt cacgtgaatg
     7201 gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag gtgaattaga
     7261 tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc cttcagcagg
     7321 aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag gaaaacagac
     7381 agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg aaagcagata
     7441 gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc ggtgaaatag
     7501 ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta tttgagcgac
     7561 agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa ggatgcgaaa
     7621 aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat tggtattctt
     7681 ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt ggtcttgatt
     7741 tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac cacttactta
     7801 ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt tcagaaaaag
     7861 attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa gagctatcag
     7921 ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga ttaggtaaat
     7981 aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt gtataaaagt
     8041 aaactgtttg attaataaat gaatcttagt accaaccgtt tgcttcccag aatgctttcg
     8101 ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt tcttggtttg
     8161 cagcagagta gtcaccattt aagta
//
LOCUS       DAZUMB010000113         8127 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000113 DAZUMB010000000
VERSION     DAZUMB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.116"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1248
                     /locus_tag="ACG80L_002258"
     CDS             94..1248
                     /locus_tag="ACG80L_002258"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS5024468.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="ACG80L_002259"
     CDS             complement(1270..2022)
                     /locus_tag="ACG80L_002259"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5024469.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="ACG80L_002260"
     CDS             complement(2386..5052)
                     /locus_tag="ACG80L_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5024470.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="ACG80L_002261"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="ACG80L_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5024471.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACG80L_002262"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACG80L_002262"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS5024472.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2358 a         1692 c         1450 g         2627 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgca
//
LOCUS       DAZUMB010000114         8059 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000114 DAZUMB010000000
VERSION     DAZUMB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.117"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..853
                     /locus_tag="ACG80L_002263"
     CDS             152..853
                     /locus_tag="ACG80L_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5024473.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1462..2478)
                     /gene="tsaD"
                     /locus_tag="ACG80L_002264"
     CDS             complement(1462..2478)
                     /gene="tsaD"
                     /locus_tag="ACG80L_002264"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS5024474.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2475..2939)
                     /gene="rimI"
                     /locus_tag="ACG80L_002265"
     CDS             complement(2475..2939)
                     /gene="rimI"
                     /locus_tag="ACG80L_002265"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5024475.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2946..3488)
                     /gene="rimI"
                     /locus_tag="ACG80L_002266"
     CDS             complement(2946..3488)
                     /gene="rimI"
                     /locus_tag="ACG80L_002266"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5024476.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3472..4194)
                     /gene="tsaB"
                     /locus_tag="ACG80L_002267"
     CDS             complement(3472..4194)
                     /gene="tsaB"
                     /locus_tag="ACG80L_002267"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS5024477.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4385..5365)
                     /locus_tag="ACG80L_002268"
     CDS             complement(4385..5365)
                     /locus_tag="ACG80L_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5024478.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5389..6873)
                     /gene="galT"
                     /locus_tag="ACG80L_002269"
     CDS             complement(5389..6873)
                     /gene="galT"
                     /locus_tag="ACG80L_002269"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS5024479.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6885..7874)
                     /gene="galE"
                     /locus_tag="ACG80L_002270"
     CDS             complement(6885..7874)
                     /gene="galE"
                     /locus_tag="ACG80L_002270"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS5024480.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2284 a         1714 c         1415 g         2646 t
ORIGIN      
        1 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
       61 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
      121 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
      181 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
      241 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
      301 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
      361 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
      421 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
      481 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
      541 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
      601 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
      661 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
      721 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
      781 cgggctgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
      841 tatgagagaa taacgcgaac aaaaataagc agtgaccctg ataaatatca gggtcactgc
      901 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg gacaagactc
      961 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgtta
     1021 gattaactag atgaagcgtc ttgtcctcat ccgatctaga ttattcgata ttcctcgaca
     1081 ataaacgatt actcagtgag ggcaatcaga ggaacggata atgataaata agctgtaaaa
     1141 aatagcaaaa taacaataat gattaaaagt atgtaaaaaa tagggctttg acaaaaacgt
     1201 gtcacagccc tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata
     1261 atgttcataa acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg
     1321 acaggcttac gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa
     1381 cagccaggtc aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag
     1441 ggcagctttt tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg
     1501 atagctagca aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc
     1561 attatctcca caaagtcgaa gagggggaat cactagttct acctcaggga gtttagctgc
     1621 caaagcatcc tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctataat
     1681 cagttgcttg actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat
     1741 cacacttgct tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg
     1801 tgcattatgc accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt
     1861 atcttcatgg atcatcgctc gcggaaaatg gtaattatct tttccctgat gagctagctg
     1921 atcgatttct tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc
     1981 ttctcctgct gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat
     2041 atagactaat tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg
     2101 tttaaccaga cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa
     2161 ttggtgtgcc caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag
     2221 tcctggtcca taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac
     2281 cagtgcatct tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg
     2341 tactacgcca ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat
     2401 ctctgttcca tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag
     2461 aatcaattct tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca
     2521 tcttccgtcg gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat
     2581 tcataaagtt ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa
     2641 tcttttattt gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt
     2701 tctgaaactg caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca
     2761 ataaagccac agatctggtt atcttcagct aaaagcagat aatcagagtg aggctgttgg
     2821 atgtctgcca aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta
     2881 atcatccata cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac
     2941 taatctcact cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg
     3001 taatatcttt ttttgatcgt tgttgcttga aaccccaatt tccgataaag tctttgtgca
     3061 tcttgattac tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag
     3121 ttctcgattt cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg
     3181 gcaatattcg tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt
     3241 cttccattat caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata
     3301 aaggcacttt ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc
     3361 tctttgatat cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgact
     3421 gttttttctg tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt
     3481 ttcaacataa ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac
     3541 tcgttttaaa tagcgaggaa gaaaagcttg tttctctgca actggttcaa ctaaagcacc
     3601 taattgagcc acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc
     3661 actaggcaat atttccttta tttcagagaa gaatttgtga caatcactcc cgacgaaata
     3721 aatagcttca ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc
     3781 ttcaagtaca gtttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt
     3841 tcttcgagca tcaaacatgg gcacaataat attatccaca ccgatgcaat tagccgcgat
     3901 ggttttcaga ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc
     3961 tgtcgttaca cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg
     4021 gtcaatgtct tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag
     4081 tgttacactg tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc
     4141 taccacaccg atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt
     4201 gatcctttct gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat
     4261 gaaaatacca aaggcaaaac tagtacaata atacatgatt atctgctggc gggcaaattt
     4321 tgccccttcc tgaaacacaa aaaagctgcg gtatcactcc attttcccac agcttttcca
     4381 tcttttattt cgttgattct cgaataatca actctgtttc cacaataatc tttcttgaaa
     4441 ccggtgcttt ggaattactt aattgaagaa tcgtctccac tgctagctct cccatccatt
     4501 cggtataaac ttttacagta gaaagcgagg gcgttacata tttcgctaca ctgacatcat
     4561 tgaagcccat cacagaaata tcttctggta ccactaaacc tttttcttga atcgctttca
     4621 gtgcgccaat cgctaatgca tcattagctg caaaaagcgc atcaggaaca tgcggattcg
     4681 tttttaaata ttgcttcact gcttgatatc ctgactcgac agaaaaatct gcttcgataa
     4741 ttggaccttc gtcgtagcct ttttctgcca aaacttgttg aaatgccata attctgggat
     4801 cttttagccg ctgatggttt ttcttcgtat attctgttcc agcaagcata gcgatatgtt
     4861 gcgctttatg ggagacaaag tgttcaatga ctttttgaac acttgactta aagtcgacaa
     4921 ctagagagtc gattccttga gaagtgccgt cagaatctac caaaagcaag gcattttggg
     4981 atcctttcat ttctaataac tgagcttcgt cgaattttcc tagtgcgatc gttccatctg
     5041 ccggaagatc actaatggaa tcccacgttt ctttcaataa agtaacgttt gcttcttctg
     5101 cctttttttc gattcctagg cgaatagata aataataaag atcttcaagc tcttccgtat
     5161 cgtcgtacca ctggatcagg cgaagcgttt gcttagtatt tgctgcattt ttacgatgtt
     5221 tcgtataatt taagcgttct gctgcttcaa aaattttttg ctttgtggct aagccaacgg
     5281 aaagatcagg atcatagttg agaactcttg aaacagtagc aggagaaact cctgccaact
     5341 gcgcaatgtc tttaattgtt gccatttgtc tctcctcctt ttttacgttt ataaaatcgc
     5401 agtaaatcgt tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc cagcgtcttc
     5461 cagtacacgt gcaaaaacct gacctacaga tttttgtacg acatcggtga ccgtatcttt
     5521 cgtcaaatca ggatgttctt tttttaattg ttctgcccac ggacgatgat attcagccat
     5581 ctggttttct tctcctaata agaaatgctc tacttctact agttctggtt tcaaacgagg
     5641 tggtaaaaca gcaagcccca ttacttcgat caacccaatg ttctcctttt tgatatgttg
     5701 gacatcttta tgtggatgaa aaatcccatc aggatgttct tctgaaacat tgttgtctcg
     5761 aaggacaagg tccaactcaa agtaattgtc ccttcttcgt gcaattggcg taatcgtatg
     5821 gtgtggtgta ccatctgctg aaaaagctct gatagatact gtttcatccg aatatgcttt
     5881 ccatttttcc aatactgagt tagctgcttc aataagcaag tttttctctg ttgattgcaa
     5941 acggatcaca gacatcggcc atttcacgac tcctgcgatg acttgcggat aatcttctag
     6001 tttgataagt ttttctatcg gtgcttttgc cataggaaat tcatgacgtc ctgcctgata
     6061 atgatcatga gaaagaatcg atcctccaac aattggtaag tcagcattcg aaccaacaaa
     6121 gtaatgaggc aagacttcag taatccgaag cagtctagca aatgtttcct ttgtgatttt
     6181 catcgggcgg tgttgttctg ataaaatgat cgaatgttca ttgtaataag cataagggga
     6241 atattggaat ccccagcttt caccgtctag attcattcga ataatccggt gattggtgcg
     6301 ggctggatag ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca tgcaggtagg
     6361 atagtctact tttaccgcct cgcgttctgc tgcgatttgt tttggatctt tttctggctt
     6421 agataaattg attgtgattt ccagttcacc atactccgac tctgccggaa atttgatgtt
     6481 ttttgcgatg gctcttgttt tgatgtagtc atttttctta cttaaattaa agaaatactc
     6541 agttgcttcc tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga cgactgaagg
     6601 tggcggtgtg agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg ctggcaattc
     6661 tgaaagaaca ccattttctc ttgccttagc gacaagctga tccaataagt ctacagattc
     6721 ttccttaact ggtcttactt caatgctttc tagagcatct tctcctacca tccctaaaag
     6781 acgattctgt aaatataagc ggtccatttc catccatccg ccagcttcga tcgcaagtgt
     6841 ggcaaaatca caaatcgttt gactgattga catatctcat tcccctattc ttcataacca
     6901 tttggatggc tgacatgcca atcccatgct gttttgataa tcgctttgat atctgtgtat
     6961 tcaggtttcc agcccagaat ctctctcgct ttttcacttg aagcgactaa tcggctagga
     7021 tctccagctc ttcttggtgc tatttctgct ggaatttctt ttcctgtcac ttctctagct
     7081 gcttctagca tttccttgac tgagtatcct ttgctgctgc ctaaattgaa gaagttgctt
     7141 tcatttcctt ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa atcttctact
     7201 tgaacataat ctctgataca tgttccatct ggtgtatcat aatcgtctcc ataaacagcc
     7261 aaggcttttc tttgaccaag agctacttgt aaaatgattg ggacaagatg cgtttctggt
     7321 gtatgatctt ctccgataga tgcatctgct tttgctccgg ctacattgaa gtagcgcaaa
     7381 gcgacatagc gcatgccata tgcttggtca caccacttca tcattttttc catcatcaat
     7441 ttactttcac catacggatt tttaggatta gctggtgtat cttctgtgat tggagactct
     7501 ttcggctctc cataagttgc tgcagtagaa gagaatacga ttttattcac gttgaactcg
     7561 tgcatgactt caagtaagat ttgcataccg tatacgttgt tgttgaaata catcaatggt
     7621 ttttcgacag attctcctac taaagagctt gcagcaaaat ggattactcc ttcaatcggt
     7681 tccttttcaa atacggaacg tagaaattct ttatcccgga tgtctccttc ataaaaatga
     7741 gcatctggat gtactgcttg tttgtgacct gtgagcaaat tatctaccac agctacttga
     7801 tacccctttt gaactagctg gtctacagcg tgcgaaccga tataaccggc tccgcctagt
     7861 actaaaattg tcataaaaaa cactcctttg attagtaaaa ttgtgtttgt tgattctata
     7921 taatttaaaa aacgaagttt acttttatgg tgaaaacaaa caaagtaaaa tttcgtaaat
     7981 tcatatggta aatttataat aaaacattta gtaaacattt tcaagggttt tttgcaaagg
     8041 tttacaaaca aacgtaaaa
//
LOCUS       DAZUMB010000115         8041 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000115 DAZUMB010000000
VERSION     DAZUMB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.118"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..427)
                     /locus_tag="ACG80L_002271"
     CDS             complement(<1..427)
                     /locus_tag="ACG80L_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS5024481.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQA"
     gene            complement(557..1267)
                     /gene="budA"
                     /locus_tag="ACG80L_002272"
     CDS             complement(557..1267)
                     /gene="budA"
                     /locus_tag="ACG80L_002272"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS5024482.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1280..2944)
                     /gene="alsS"
                     /locus_tag="ACG80L_002273"
     CDS             complement(1280..2944)
                     /gene="alsS"
                     /locus_tag="ACG80L_002273"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS5024483.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3152..3814)
                     /locus_tag="ACG80L_002274"
     CDS             complement(3152..3814)
                     /locus_tag="ACG80L_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5024484.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3824..4639)
                     /locus_tag="ACG80L_002275"
     CDS             complement(3824..4639)
                     /locus_tag="ACG80L_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5024485.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4900..5343)
                     /locus_tag="ACG80L_002276"
     CDS             complement(4900..5343)
                     /locus_tag="ACG80L_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS5024486.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5374..5487)
                     /locus_tag="ACG80L_002277"
     CDS             complement(5374..5487)
                     /locus_tag="ACG80L_002277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5024487.1"
                     /translation="MEADSSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(5477..5815)
                     /locus_tag="ACG80L_002278"
                     /pseudo
     CDS             complement(5477..5815)
                     /locus_tag="ACG80L_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5803..6180)
                     /locus_tag="ACG80L_002279"
     CDS             complement(5803..6180)
                     /locus_tag="ACG80L_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5024488.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6404..7597
                     /locus_tag="ACG80L_002280"
     CDS             6404..7597
                     /locus_tag="ACG80L_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5024489.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7763..>8041)
                     /locus_tag="ACG80L_002281"
     CDS             complement(7763..>8041)
                     /locus_tag="ACG80L_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024490.1"
                     /translation="LEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSY
                     NYHLIEFMYQHPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2349 a         1636 c         1380 g         2676 t
ORIGIN      
        1 atgcttgatc gatttcatca taccctttcg ttactccgca atcgatatct aaaacaccga
       61 ttaaattacc ttcttttacc attggcacta cgatttctga ttgtgcggct gaatcgcagg
      121 aaatgtaatt tgcatgggtt ttgacatcat caacgatcaa tgtttcttgt ttttcagctg
      181 attctccaca aactcctttt cccatcttga tacgtgtgca agaaactcgc ccttggaacg
      241 gtcctaaaat caattcatta ccatcgaata aataatatcc tgcaaagaca gtctcaggca
      301 aagtctcatt caacaatgca gatgaatttg ctaaatttgc taaccagtta tcttctgctt
      361 ctaataatcc ttgttgctgt aataataaaa tttcgtatgc atcttttttc tgtttttctg
      421 tccacatgat aaaaaccgct cctttttgat ctatggagaa gattatagct cagttttttt
      481 gtaaacgcta ggtttcctcc tcaaaaaaac gagatacgtc aggttaatca ccttgaacgc
      541 atctcgctgt ctttttctat tctgcttgtt ggatttctgc tgctaaatca gcataatcaa
      601 tcgtttcgtg aacaaaagtc ggatcatcaa ctggaaaatg ctgtacaagg gacgagatgc
      661 ggctaatctc aacttttcct tcagccactt caaaatccat tacatgtccg ccaaatgtat
      721 gtgtatcatc gataaaatgc aggtgaaatc ccgctgcagc aactccttgg aataattcag
      781 gcgtaaagaa cccgactaat gttccttgga tattgctttg ttggaattct ggttgagact
      841 ctgaaatttt tgctagtctt ctgtaaggtt tttcttgctt cggcatgata cggatgtgca
      901 tatttttgaa taatccagtt atcttcactg ccaaaaatag gttttccccg gcttctctta
      961 ctaatatgtc ttttttcaat tgttctccat ctgcagtttt tgagacagaa aaactagtat
     1021 ctggcgtaaa atctgtaatt gctgcatagg gggtaagttc acttccatct aattgggcaa
     1081 ctgagccatc tgatcttcct tgataagcta cgccatctaa aaagatgact tcaccatcaa
     1141 gcccatgcaa tgttcccaat cccagagttc ctttttctaa tatatgagca atcgtttctg
     1201 ttccatccat caagccggcc atcaaagctc ctaatgtccc gtgctggtat aagatttttt
     1261 cagtcatgtc gacacttcct taatagaatt gatctggtaa gattgtttct cccaatttct
     1321 cattatcacg gtaatcaatc ggaatatcaa taattacggg tccttctgtc gcaaatcctt
     1381 cttctaaagc tttttctaat tcctctacac ttgtagctcg gattccttta gcgccaaaag
     1441 cttctgcata tttcacaaaa tcaactggac caaagtctac acctgacgcc cgctgatatt
     1501 tcattttttc ttggaactct accatattgt agtgtccatc attccaaatc aaatgaacga
     1561 tattcaattt tttacgtacc gccgtttcta aatcctgggc agagaataaa aatcccccat
     1621 cgccggaaac tgatacgatt tgtgtatttg gacgcaccaa tgcagcagaa atcgcccatg
     1681 gcaatgcaac accaagcgtt tgcataccat tactgaataa caaatgacga ggttcataac
     1741 ttctaaagtg gcgtgccatc caaatataat gactacctac atctacggta acagtcatat
     1801 catctgttac ttttgactga agcgtgttaa taacttctaa aggatgcaag atacctgctt
     1861 ctccttttga ttgaacaatg tcccgctccg ttaattttgc ttgaagagat gctaaatatt
     1921 cttttgcatc ttctgacact tgttgaggct ctaatgaacc tgtcaataaa tcaagagtgg
     1981 ctgagatatc accgatcaat tctctttcag gctgcataaa aggatcgatt tcagctggcg
     2041 cttcatcgat cacaatgatc cgcgcatctt tttccgcatt ccagtttctt gcttcatatt
     2101 cgattggatc atagccaatt gcaataacta gatcacttcg tttcaaaagc atatctcctg
     2161 gttggtttct aaataaaccc acacgaccaa agaaatgcgc ttctaattca cgtgagatca
     2221 ctccagctgc ttggaatgtc tctacaacag gcaatgctgt cttttcaact aattgcctga
     2281 tggctaacgt ttctttttca cttgatcctc gcatacctac taatagtacc ggtagttttg
     2341 cttctctaat gcgttcagct aggtaacgga tatcatctgc agaagcagaa cctaatttag
     2401 gatcgctcaa tggcttgatg acattacctt ggacaggtgc atccactaca tcttgcggga
     2461 tactgataaa gctcgcgcct ttttttgaac tcttagccat acgataagca ttggcaatca
     2521 cttctgaaat tgtttctgga tcttggattt ctgcactgta ttttgtgatt ggctggaaca
     2581 gtgctgcatt atccatgctt tgatgggtca acttcaatag atcgcttcgt tttacttgtc
     2641 cggcaattgc taaaactggg tcaccttctg ctgtcgctgt taccaaacct gtagctaagt
     2701 tactagcacc aggaccactt gttgcgataa ctactcctgg ctctcctgta atacgtccga
     2761 ttgcttgtgc cataaatgca gcattttgtt cgtgacgagt cacgattaat tcaggacctt
     2821 gatcctctaa ttcattaaat acaccatcga tttttgcacc tggtataccg aatacataat
     2881 caacatgatg attaatcaaa ctttctacta cagatttcga accttgtttc attttttcag
     2941 ccattgaaca tcgtcctttt tatgctttat ttttaattat tagttagaaa taatcacaaa
     3001 taagtgatac agtttctaaa cctgtttcat acaaaagtat agcacgtttt atttcatttg
     3061 tttagccata tgtttcaaaa aatgaaaaag aatgacagtt tttacataaa aaaaactgtc
     3121 attcttaatt gaaagtttca atttataaag tctacagatc catagttctc atcaatttac
     3181 gattgttgaa gtaaatcaaa ccaaatacga tagaaggacc tagtagaata caccattggg
     3241 gctttacgaa aaagaagaga aacaaacttg tcaatagtaa aaaatctagt aaactcacat
     3301 accatttttt caacgtatag tagaacgcta tccgtagaac ttgatttggt ttcgacgctg
     3361 gatttctcac ctgaaaatac cagcagttgg tgctaaagga aactgataaa caagcaagta
     3421 aaacaaacaa cggaatcaac cattttccat aagaaaaacg tataaaaaag aaacaatcaa
     3481 aaatcattat cacactaact atccaagcaa atagccaata aaaaaatccc tttagccaaa
     3541 attggcgaaa catctttatg aacaaacgta ctggtgaaaa actattttct tttgctaatt
     3601 cttctgtcat acctatcatc acgatgcaac ctggaccaat aaaaagtaaa gaaaaaataa
     3661 aggaaaagac attccttcca tctatcgaca aaaggaacgc agctgctata tacaagatat
     3721 tgacacacca aaaacaaagg ctacttgcca atgattgata caaccaataa aagaattcca
     3781 tataaacatt tttgtcaaaa cgctgctctt tcataatcac ctcctattcc ttcattcctg
     3841 atgaagcgat cccttctaca aaatattttt gcatcgctaa aaatataaaa gcaacaggaa
     3901 gaacagaaac aactgaggca gccatgatta atgcgtactc tgcatcaaat agcccaataa
     3961 acattttcaa ccctaactgg attgttttat tttccgtaga agacaaataa atgaatggcc
     4021 ccatataatc gttccatgta ttaacgaatg tgatgatcgt caaactggca atcgccggtt
     4081 ttgtaagagg taaaatgatc ttgcgataga ttccccactc tgataagcca tcgatccgcg
     4141 cactctcaca taaagaatct gggatactac tgtaatattg cttcaacaag aacacaccaa
     4201 aagcattgaa cgcttgtaat agcaccaacg accacaacgt atctgttaaa cccacttttc
     4261 tcatcataat gaactgcggg atcatataag actgccaagg gacagcaatc gtgccgatat
     4321 atgctaaaaa taaaatatcg cgccctttaa aatttagttt agaaaacccg taagcagcaa
     4381 aactagaagt aaacaactga atcaacgtga tcacaacgct taaaaaagct gtattcttaa
     4441 aaaatgtcac taaaggaata cgagaccaaa taaccagata attctctaaa tgccaagttt
     4501 tagggatcca ttgaatcgga atcgtgaaca cttcattatt tgctttgaat gaagcagaaa
     4561 ccatccataa aaaaggaatg atcgtcaata aagctaatac aatcagcaga atcataccca
     4621 accattgaat aaagggcatg ttttttgaac gaatttttgc tttttttctt tcatttcgaa
     4681 cacctccaaa gttataatct tcatttcttt gtaatgatta caatccagaa caacggttat
     4741 tatagctgat tcatttccgt tcttcttatt tttgtaagcg cttatttaac aagttatgta
     4801 aatctcttta tttttttgat ttaaaaatga aaaaatacaa aagcaagact gtcatacgtc
     4861 tcgcttttgt attgaaattt cttcgattag agatacttat tactgttcaa aagcttcttt
     4921 gaaacggtga atcaattcat tgactacagg tgataaaatc cgattctttt tccagactag
     4981 catgcagttc gtttcaatca ttgggtcaag cggaacgaat gtcactcgct ttgaatgatg
     5041 acttacagca ccttctatga ctaaagcagc acctacctga ttctcaatta gcgcaaatac
     5101 attgaaaatc aaattgtacg ttcctgtgat cagcaactgc tcctttttta tgcctaacca
     5161 gtcagcaaac attcgctgta tttctggacg ctttgagatc aaaagaggta cattcgcaaa
     5221 gtcttctttt gttatcacgt ctttttttgc taaaacagaa ttttctaaaa tgagcaatcc
     5281 ccatttttcc tttcttggta acaaaatcgt ttcgtatttc tctgtatcta ctggatctag
     5341 caaaatcgct aaatctaaca ggcttttttc tagtcactcc gtgatatcat cacttgttcc
     5401 gctgtggata tgaaattgaa cgctgggata atcacggatc aacttttcta gtaatagtgc
     5461 caatgtattc gaactatcag cttccacaca gccgatcgta aatcggccgc tgaataattg
     5521 tttttttctg ttctcgaatt cttgagtggt ttgattcgtt aaatctaaaa tttcttctgc
     5581 tcggcttttt tagaaaataa cctgcttctg tcaagatcat tttgcggttt tcgcgtacaa
     5641 acagttctgt tcctagttac tcttctaact ccttcagttg tcggcttaag gttggttgtg
     5701 taagatgtag aacttctgcg gcttgagaga ttgttccttc ttctgcaatt gtccaaaaat
     5761 attttagtaa gcgtaactcc atagcgatgc tccttgattt ttttacttga tacattccat
     5821 tttatttcta gataacacac gatcatattt cttgattttt ccttgtaaac gttccaaaca
     5881 ttcttgcatt tcagcaattt ttttcgtcaa aagttcactt tcttcaatca aaagcttacg
     5941 ccgctcaaat acagttgctt ctccttcgct agataggcga atatattcaa tcaaagactc
     6001 gatcgataat ccggcatttc gcatacagat cgtaaagtcg acccatttta ggtcttcttc
     6061 tgtataatca cggattccgc cttttgtccg attaactggt ggaatcaacc cgattcgttc
     6121 ataatagcgc aatgtatcgg ccgaaatgcc cttttcttca ctgacttgtt tgatattcat
     6181 ctgatcactc tttctcttaa tttcttgcca ctttacttat gtattttatt tattctacaa
     6241 ctttaagtgt actccaagtc aatactcatt tctattaaaa ctaagctgga ttatctatat
     6301 cggttgtttg tgtattattt caaataaaaa agtcttgttt ttttattctt ttttggatat
     6361 acttttaaca atttagggaa attttaggaa aaggggtata aaaatgacaa cgtatcagcg
     6421 aaaggtatta gtatcgacat cggcgggaat tgctttagag aatatggata ttatgttttt
     6481 agctttttca ttatcttcaa tgattacttc ttttcatatt agtggtacgc aagcgggttt
     6541 catttcaacg atcactaatt tggggatgct cattggaggt attttttttg gcatcatagc
     6601 tgataaatat ggacgtgtga aagtattttc tcaaacggtc cttctgtttt cgattgcttc
     6661 tttattgatg tattttgctt caaacatcta cttggtttat ctctttcgct ttatcgcagg
     6721 tgtaggagcg ggaggagaat atggggcgtg tatgtccttg atttccgaaa gtttttcaaa
     6781 aaaacaaatt ggacgagctt cttcggttgc tggtattggt gcgcaggtcg gtgcggcttt
     6841 ggctgcgatt ttagctgctg tgatcattcc aacgtttggt tggaagatgt tgtatgtcgt
     6901 aggtgtactg ccggtgttga tggtcttgtt cattcgtcgc ggcttgactg aaccaaagga
     6961 attcaaatct gtacaatctg ccgaaaagaa aaccaaactt agcagactgt ttgaatcaaa
     7021 acatcttgct tggcaaacat gtggccttag tatcatggta actgtccaaa ttgccggcta
     7081 ttttgggtta atgaattggc taccttcaat tatgcaaaat cgattagggc tttcggtttc
     7141 tgggtcttca ttgtggatgg tcagtactat tttaggcatg tctttgggga tgatgacttt
     7201 tggcataatc atggataaat tggggcctaa actagccttt tctttgtttt taatttgttc
     7261 atccttatct gtctatctac ttgtgttagc ccatagccag tggagtttgg tagcagccgc
     7321 tgtgatagtc ggctacttta tcaatggcat gtatggtgga tatggcgcag tgattagtag
     7381 tttgtatcca acagaaatcc gcgcgacggc gaataatttc gttatgaacc ttggacgagc
     7441 tgttggtggt ttttcttcta tcgtcatcgg tttcttgatg gatcattata gtttaactgc
     7501 cgtaatcttg tttttgagcg gtatttacct agtcagtctg ctggtcttga caactcttac
     7561 tggtgttaca gcattaaggg aaagcttgca gcattgatag ttgagtctgt aaatgacaag
     7621 aaagaggtgt ggaaaaagct tgcatctgcg gtaataagac cagccaaatc aaaaacatga
     7681 gacactgttt ccgtttggct ggtcgtatta cttgatgcag ggcagctttt tcaactgcta
     7741 atttccttct atctggcggc gactattctt tgacgtccgt catttcaaag tactttacta
     7801 ataacttagc ataacgaaaa actgtggata cactcgtata ttgagcgtct gcataatctt
     7861 ccaaattttt ctcaggatgt tgatacatga attcaatcaa atgataatta tatgattttc
     7921 tgtagaaatg aagataaata atgtagattg ggaagttctc ctgcttttcc atagaaaagc
     7981 gccgattatt ctcattcttc aaaatgacct tatcaggata ttgttggaac aattcttcta
     8041 a
//
LOCUS       DAZUMB010000116         7995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000116 DAZUMB010000000
VERSION     DAZUMB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.119"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..572
                     /locus_tag="ACG80L_002282"
     CDS             384..572
                     /locus_tag="ACG80L_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS5024491.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="ACG80L_002283"
     CDS             650..1663
                     /locus_tag="ACG80L_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5024492.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="ACG80L_002284"
     CDS             complement(1730..2764)
                     /locus_tag="ACG80L_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5024493.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2879..5104
                     /locus_tag="ACG80L_002285"
     CDS             2879..5104
                     /locus_tag="ACG80L_002285"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5024494.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5122..7005
                     /locus_tag="ACG80L_002286"
     CDS             5122..7005
                     /locus_tag="ACG80L_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS5024495.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6986..7960
                     /locus_tag="ACG80L_002287"
     CDS             6986..7960
                     /locus_tag="ACG80L_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS5024496.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2578 a         1458 c         1696 g         2263 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa
     2821 cagtatacag gaattataaa aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat
     2881 ggatattacg catagaaaaa cagataaaaa tttcatctta tttgatgaaa agagccgagt
     2941 cttccattta cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt
     3001 ggcgcatatt tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg
     3061 aagagatcgt ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga
     3121 tacactaccg caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat
     3181 cattcagcgc aagaatggat cagacttact ggatctgaga tatgacagcc attatattac
     3241 agatgggaag ccagagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc
     3301 acaaacgttg gtgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta
     3361 taccatattt actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg
     3421 agaaacaatc aaactagaaa aagcggctag tttccagcta gactttgccc atactaggcg
     3481 atttgatgaa gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca
     3541 atctgtttta tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat
     3601 gaataacttt attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg
     3661 acttcaattt gtttattccg gcaaccattc tttcgaactt gaaaaagatc agatcaatca
     3721 attgagagtc gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca
     3781 gtcttttcaa acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag
     3841 tcagatccac catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga
     3901 acggccgatt ctggtcaata actgggaagc cacttacttt gacttcaata gtgaaaaaat
     3961 caaggcgatc attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg
     4021 ctggtttgga aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg
     4081 caagttgaca aatggattac gtgaaatagc agattatgct catagcaaag gattgaaatt
     4141 tggtttatgg tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacatcc
     4201 tgattttctt atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact
     4261 ggattttaca aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga
     4321 tacgattcct cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc
     4381 gattaccctt gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta
     4441 tgaactactt gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg
     4501 tggaggtgga cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga
     4561 taacacggat gcagtggagc gtatgaaaat ccaatatgga acaagccttg cttatccgat
     4621 ttcgtccatc acagctcatg tatcagctgt accaaatcat cagaccgaca gatctacttc
     4681 gttgaaaaca agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac
     4741 aaagctgtct cctgaagaaa aaaagcaagt aaaagaccaa attatatcct atcaaacgat
     4801 tcgtccggtg atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat
     4861 tgccgcatgg atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat
     4921 actagcttca gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc
     4981 actttatcag gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg
     5041 actttacttc cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt
     5101 atgagagaga aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt
     5161 tttagagtga accaatcaaa tgcacatagc gatcatgttt gttatgcaac aagggaagaa
     5221 gtagaaacca catcctccct taaacagtct ttaaatggat catggcgttt ttgttattcg
     5281 ccaaatcctt cggtacgacc aaaaacattt tataaaaaag attttgatgc ctgttctttt
     5341 gatgaaatca ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc
     5401 aatacgatgt atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct
     5461 gagaaagagg cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg
     5521 acgacttttc gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt
     5581 gtagaacaag caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg
     5641 ttcactccgt ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg
     5701 gaggtttaca aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt
     5761 gggatcttca gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg
     5821 atcaaacctc gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt
     5881 gagcaagtag ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa
     5941 atctatcaac agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa
     6001 aatgtggcac tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata
     6061 gacgaagaaa aaagagtaac ggagtatatt gactattcct tcggttttcg taaaatcgaa
     6121 atcaaagata aaatcatcta cttcaatgga aaacggatga ttatcaacgg ggtaaatcga
     6181 catgagtggc atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg
     6241 gctatcataa aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc
     6301 acttggtatt atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa
     6361 tcccatggct catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca
     6421 gtacctcagt ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa
     6481 aaccatccaa gtattctttt ttggtctctt ggaaatgaat cttatgcgga agaaaacatt
     6541 cgacaaatga acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg
     6601 aattttccaa atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg
     6661 ccaccagaag agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt
     6721 gaatatatgc atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt
     6781 gatcgttatg aaatgtacca aggaggctat atatgggatt ttaaagatca ggcgatccaa
     6841 gtgactgatc caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa
     6901 ccaagtgact atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc
     6961 gccatgcagg aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac
     7021 ggaaaaagtt gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca
     7081 ttatattttt tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg
     7141 gctataccgg gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag
     7201 cggatttgga tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga
     7261 aatagaagta ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca
     7321 atattcaagt aatgaatacg ctgaaacggt tgaaatcatt tttacactgg aaatcaacac
     7381 agtaccaaaa acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt
     7441 caatatgcat tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt
     7501 cattatgcca acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc
     7561 ggatcggatg gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc
     7621 ataccttgtt ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag
     7681 aaatcaaacg ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca
     7741 gcaggatacg ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc
     7801 aacacatata gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt
     7861 aagaggagtc gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag
     7921 cagcgaagag gaccatgaaa tcggctttat tattcagtag caatctttag attattattt
     7981 gaaaaacgga gttta
//
LOCUS       DAZUMB010000117         7899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000117 DAZUMB010000000
VERSION     DAZUMB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.120"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..995)
                     /locus_tag="ACG80L_002288"
     CDS             complement(21..995)
                     /locus_tag="ACG80L_002288"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5024497.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ACG80L_002289"
     CDS             complement(1075..1950)
                     /locus_tag="ACG80L_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS5024498.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ACG80L_002290"
     CDS             2080..3987
                     /locus_tag="ACG80L_002290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS5024499.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="ACG80L_002291"
     CDS             complement(4528..7806)
                     /locus_tag="ACG80L_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS5024500.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2235 a         1665 c         1465 g         2534 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtg
//
LOCUS       DAZUMB010000118         7880 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000118 DAZUMB010000000
VERSION     DAZUMB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.121"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACG80L_002292"
     CDS             <1..152
                     /locus_tag="ACG80L_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5024501.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ACG80L_002293"
     CDS             complement(230..763)
                     /locus_tag="ACG80L_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS5024502.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACG80L_002294"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACG80L_002294"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS5024503.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACG80L_002295"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACG80L_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS5024504.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ACG80L_002296"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ACG80L_002296"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5024505.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ACG80L_002297"
     CDS             complement(4488..5066)
                     /locus_tag="ACG80L_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS5024506.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ACG80L_002298"
     CDS             5388..5858
                     /locus_tag="ACG80L_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS5024507.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ACG80L_002299"
     CDS             complement(5966..7192)
                     /locus_tag="ACG80L_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5024508.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2299 a         1573 c         1326 g         2682 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta
//
LOCUS       DAZUMB010000119         7856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000119 DAZUMB010000000
VERSION     DAZUMB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.122"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG80L_002300"
     CDS             278..787
                     /locus_tag="ACG80L_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5024509.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG80L_002301"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG80L_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5024510.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG80L_002302"
     CDS             2322..3302
                     /locus_tag="ACG80L_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5024511.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG80L_002303"
     CDS             3424..4842
                     /locus_tag="ACG80L_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5024512.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG80L_002304"
     CDS             4913..6382
                     /locus_tag="ACG80L_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5024513.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG80L_002305"
     CDS             6392..6859
                     /locus_tag="ACG80L_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5024514.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG80L_002306"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG80L_002306"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5024515.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT         2685 a         1184 c         1603 g         2384 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaa
//
LOCUS       DAZUMB010000120         7683 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000120 DAZUMB010000000
VERSION     DAZUMB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.123"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..2381
                     /locus_tag="ACG80L_002307"
     CDS             54..2381
                     /locus_tag="ACG80L_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS5024516.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2378..3292
                     /locus_tag="ACG80L_002308"
                     /pseudo
     CDS             2378..3292
                     /locus_tag="ACG80L_002308"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3365..3880
                     /locus_tag="ACG80L_002309"
     CDS             3365..3880
                     /locus_tag="ACG80L_002309"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS5024517.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3877..4323
                     /locus_tag="ACG80L_002310"
     CDS             3877..4323
                     /locus_tag="ACG80L_002310"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5024518.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4473..5288
                     /locus_tag="ACG80L_002311"
     CDS             4473..5288
                     /locus_tag="ACG80L_002311"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5024519.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5494..6666
                     /locus_tag="ACG80L_002312"
     CDS             5494..6666
                     /locus_tag="ACG80L_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5024520.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6828..7535)
                     /locus_tag="ACG80L_002313"
     CDS             complement(6828..7535)
                     /locus_tag="ACG80L_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024521.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
BASE COUNT         2587 a         1451 c         1532 g         2113 t
ORIGIN      
        1 aatttagaat aacgtgtaaa ggattatttg atgatttttt attgagagga gggatgaaaa
       61 tgattaagaa aactcaaaat aaaatccaac gtatatgtca aaaacatttt gtttctagac
      121 tcaaagctgt atctaaaaga aaatacttac atcccattat gatttcgatc atcttgattt
      181 ttactttctt agggagctta tcaagtattt atgtaaacgt ttatacagaa acgtcaaatt
      241 ttgctaacca aacgacagag gagacaaata gtccacccta ttctgaaata cagtccatag
      301 aaagcaccaa tcagtctagc aacaacgaga caacctcaac cacatcctcg actcaactga
      361 ttgatccgac agaaaaagaa atcacagcag caaaagcagc aggggagaaa gcttattcag
      421 aaacaggtag aagccagaca tttagtactg ttgcggccac tgcgaatttc aacggacata
      481 ccataccaga tggatataca gtgatatatg ccaaagatgg agtcattgtt gcggttaacg
      541 gatcgatgag tgcggataaa actgcgttac aaggcgtaac caaagcaatc gcttttattg
      601 ataaccgtcc agatatccat aatctgttta ttggctccac agtaaatggt actactatca
      661 cattcccaaa aagtatctat atgggggtgg ctaccgctgg aaatacgatc gattcagtta
      721 cacccaatgc ggataaaagt tattctttta caccctcaac atggggaggg cttcctgccg
      781 gaggaggtat gagtactttt tcaatcggta tacagttagc aaatgatatt ccattcacgg
      841 ggtactcgat caaaaatggg cagatcgttg atggtaaggg aaatgtttca aattttccaa
      901 cctatcaagc agcaacggtt caggtcaatg cgaatcggac aggaactttt tcttatacag
      961 tcaatggtgg agcagcaaaa atcggaacgt acgggactac attatctgga atccatgtgg
     1021 gtgacaaagt ggttatcact ccaacagcag taacgggcta ttcttttgca caaacaaacg
     1081 gttctttcac agccctagaa agtaacgtcg atacggtgac ctatacaggc aaacaaacac
     1141 caatcactat taagtatcaa gactattttg gacagatgat tgctccttct aaaataatga
     1201 atcttactta cggaagtgct gcacaagacc tgacgactaa tacgcctact atcaacggat
     1261 atacctttac agctgtttca gcgatagaaa caaagaacca atcagcaatg agtgttttag
     1321 ctagtttaga tacaaatgga aatgtaatcg taaaggacgc aaatggaaaa cagatttctg
     1381 aagtcatcct atattataag acaaatgcaa cagtatcaat caatgcaaat ggcagtaagt
     1441 actatgatgg tctttctgtt gtaccgatcg ttaaatatgc ctttaatgat aaaacagaat
     1501 caacatccct tttaaacaat gatctcagta ttgatcaaat cacatggaat ccaactgatt
     1561 tcaaagcgat gaatgaaaag ggagttgaag taactgcgcc tactgaaatt ggaacctata
     1621 cgtcttggca attgacaaaa agcggactag ctaaactagc tgccagaaca aactatttat
     1681 ttactatcgt gcagacatct gatgtctata cgattaagca aatcagcggt gcggtagagc
     1741 tcagtggcag taagacatat gatggaaaag caggtgtccc gtcaatccat gtcaagctag
     1801 cagagggtgt cactagttcg cttactccag tacaagtggc acttagcagt atggattaca
     1861 cagcagatgc acaatcagca aaaaatatgg ttaatgctgg cagctataca attaaactaa
     1921 cgaatagcgg aatagacaaa gtcaaagcag caaatcccaa gttgtcattt actgatatag
     1981 ccaacacatc aggaacttat accatctata aagctaacgc agtgattact gtcgatgacg
     2041 ctgcctttaa ttatgatgcc caaagtcact cgatttcggt tggaaatctc cacgttaccg
     2101 gtgttgtctt aggcgaatca ttggattata ccctaacagt taacagccgg acagatattg
     2161 gatcacaaat agtaacgatt agtttaggcg aaggaagtgt aaacaataat tatacgatta
     2221 tcgcaacaga taccgctaag ttgacgatca atccgactct cttgcttcct tcgacaggtg
     2281 ggataggtct actgcccttt gtctttttag gactgatctt tattttttct ggcttctttt
     2341 attttatcca tcgtaagaaa aaagcaggtg aacagcgatg aaaaaaacaa aaaatgtacg
     2401 tgtggatata tttgctgttc tcttttttat cctactggtc ttttttatag gttctccgac
     2461 tgtgtcagct agagaatcac ctattacatt tactttgcat aatcgaatgt tcgaaagcga
     2521 taagatgcca agtatcagtc aaaacgacgg aataaatatc ccggataact ttcaaggagt
     2581 aaaagggttg aacggcgtta ctttcgctat atatgatgtc tcggatgaat tttacaagct
     2641 gcggtcagaa ggaagtagtg tggaagacgc acaaagaaaa ctagcacaaa aaagtgatag
     2701 tgaaaaaata ctggcagaaa atgtgacaaa aacagtagac gaggaagagg gcattgcttc
     2761 ttttagtgca tcggacaaag atgagcaagg tcgtgacgct gtttaccgat ttacggaaat
     2821 aaagacttca gattaagtaa aagaaaaatc agcaccattt gtcgttgtgc ttcctgtgac
     2881 agattcatct aatcataaat tgacgaccat ccacttgtat ccaaagagcg aacaaaaaat
     2941 acctgctgca ctcacattaa caaaaacagt agagaataag cagacagatt ttgcagatgg
     3001 ggataaagtc ccttatctca tcaccgtaac aattcctgag aacatcaacg agataggaac
     3061 ctatacgatc aaagatacag ctgatcctca gttatggcta gaaccagaaa cgatcgattt
     3121 tttgattggt ggagacaaga ttcatacatt tcacacacaa aaaataaaac atggttttgt
     3181 cttatcagat tctggaaaag atcttagttc gtttgctgga aaaaaattaa cgatccgtta
     3241 ccaaatgaca ttgaaagaaa acagtgaaaa aacgacattt gataacgatg tacgtttgac
     3301 gactgacaat caaacagtag aaacacatct tgccatccaa acaggaggca aacaatttgt
     3361 caaagtggat cggcaaacga aacaaaaact agcagatgct caatttctag tcaaaaaaaa
     3421 tgaccaatac ttagcgcaag aaaacgggat aaatcattgg gtagccaaag atgattcaag
     3481 agcaacgatt tttacttcca gtaaagatgg aacattttct gtccatggat tgtcttttgg
     3541 aagttacgaa ctagtggaga ttaaaccgcc taaaggatat atattgagtc aatcaaccat
     3601 tctctttgaa gttgaaaaag gcagttatga tccgcttcat cctataccat tggaaatcat
     3661 caacgaacca aaagcaaatc cgacgaataa tggaaaaacg ccagttcaga tgcagacaaa
     3721 tggtccatca aatgaaaaaa atggaggaag ctttccaaaa acaagcgaag agatgcttgg
     3781 aggtttctca attttagggt tattgctagt cctttcaaca gggacagctt ggttttataa
     3841 aaaacaacaa aaaggaaaca ataggaggga aataaaatga aaaacatcaa aacaaaaata
     3901 tggacgtttt taggaactgc tatcatgctg cttcctttcg ttctcggcct aggaactgca
     3961 gaggtttctg cagccgtatc acctacacca gaaaatgtta cggttaattt gcataaattg
     4021 aaattcactt cagcaccaga aaatcaaata aacaacggaa cggaattgac atttccgaat
     4081 agcgaaccgt tgaacggcgt tgaattcaac gtatatgaca tcactgctac gtactatcca
     4141 agtaaagaca cagcagttcc agcagacgct acacccttcg caagtgtaac gacttctggt
     4201 gaaggattag ctaacctgac tcttccaggt aaatctgatg ggaaagatgc tgtatacgtc
     4261 tttgtagaaa caccaaagcc cggtgtcgaa acgtcgccaa atatcgtgtt aagcttaccc
     4321 tagaccctga caaaccttta gacacgattc acctatatgc taaaaatgtc atcaaagaaa
     4381 gcaatatcaa aatccagaaa gctgggaact tttcaggggt agataagtta gcaggggcgc
     4441 aattccagat caaaaattca gagaatcaat tcgtgaccgg ttttgatgaa aacactggtg
     4501 tcgctctcta tgatcctgct gagtcagcgt tgacattcac aactgatact gaaggatcca
     4561 tcagtgtcaa tggattaata gacggcaatt atactttagt tgaaacaaaa gcaccagatg
     4621 gttatcaatt aaccaatcca caaaaagaaa ctacttttat agtaaaaaac ggaaaattgc
     4681 aacaaacaac cggtatcatc catggcgcag atgaagccaa tcttgggaat ccaaacggtg
     4741 tagtcaatcg ggaaaataca actacggata acacgattac tgtagaaaat gtccaaaact
     4801 acggtgattt ccacttcatc aaacaagatg tgaacacgca agaaaaatta gccgatgcag
     4861 aattttctat tctaagagca aaagatccaa ctgttaaatt aatgaaaaaa atagcaaacc
     4921 ttgcctcagg agaataccca tatgcttggt cagatgaagt cacaggttct gactgggaag
     4981 aagtgaaatt gatttctgat tcagaaggga aattttcaat cgaagggctg aaacaagggg
     5041 catactgcct tcaagaaaca aaagcaccag aaggctacgc attacctaag aatgaagctg
     5101 cgtacactga atttacagta gatgatgatc caacaacttc tgatgaaaca acgatcgata
     5161 accaacctaa aggaatcttg ccttcaactg gtggtatggg gatcatcgta ttcatcttgg
     5221 taggtactgc tttagttgga ggagcagtga tctatttcaa aaaacgccat caagaaacag
     5281 aagcttaaac tattggaaac aaaaatcata cgataaataa acgatagtga ttcaaatggg
     5341 gagaaaggtt gtgacaagtg gtcggtctct tggtattcgc tcaacaaacg gcaaaatagg
     5401 aggaactatt ttgccgtttg cttcgctaat actcaggtca aggcgctgat ttcacaacct
     5461 cttcatatac atattagaaa ataggtggac atgatgaata aaagcagaat aagaatactg
     5521 gacatcttca tggcaatcat tttagtcgtt gggatcggga tcttttctta tccttttgta
     5581 gaagattcac tgaatgattt tcttgcccag caaatgatca tccattatca aaagcaagca
     5641 tcgaataaga attcagagga aataaaaaaa cagcaggaaa aaatgacaaa gaaaaaccaa
     5701 cagttggcag agcaaaacgt atcaccaggg ataggcagtt tcaaccaagc agttgatgca
     5761 aaagcattaa aagatttacc tagtaatgcc ttttttatgg aacatatgct tggagtcatc
     5821 gaaatcccaa aaatcaatgt cagcttacct atattcgatc agacaacgga aatatttttg
     5881 caaaaaggaa cgtcattatt ggaaggaagt tcttatccga ctggcgggaa aaacacccat
     5941 gccgttcttt ctgggcatcg tggactccca gaagcaaaat tatttacaga tttgcctaag
     6001 ttgaaaaaag gagaccagtt tttcatccag atcaatggaa agacattggc gtatcaagta
     6061 gaaaaaatcc aagtggtctt acctgacgaa gtagattctt tgggtatcca aaaaggcaga
     6121 gatctcgtga ctttgcttac ctgcactcct tatatggtca atacacatcg actgcttgtg
     6181 acggggcatc gtattcctta tcaagcaaaa gaggcaaaaa aagcgatcca aggaattgat
     6241 caatggaaaa aatggaaatt ttttatttgg tttatcggta tactacttgg atcaatcggc
     6301 ttagtttggc tactcatcgc ttatttagat tctttggcca tcgctaagcg aaactatcct
     6361 ttatcttttt atgtaaagaa cacaaacgga cgaccaatcg aaggaatggt cttttctgta
     6421 aaaacgctca acggcaaaca ctatattact agagaaaaag ttccatttgt gaaagcttct
     6481 gatgaatacg gactagtgag attttctgat ttaaaaggca ggaactatcg cttgcaacat
     6541 gaggagctcc tgcttaaaat ccatgtaaaa cataaacatt cgaaacagtt ttcaatgaaa
     6601 ttgaaaaaag gaagatataa actgcgaaaa gaaaaagagg tgtactattt gattgagaaa
     6661 gaatgaatga agatgtccaa aaaagcagag ggaacagact aaatgaaaaa agagagaagc
     6721 tgacgaaatg gacgtgtaaa ctgctatttt tggtttacta tctggaacgc cagcttctat
     6781 ttttttatgt ttcagaagaa ttttattatt atagtataac ttttgaatta gtttccataa
     6841 catttggagc aaggggttaa accacgagct tgagcttcgc ttaaagtggc aggggtatac
     6901 gtaccatttc cacatttatg tgtatggtat tttgaaccgg tcggtgtgac atagacagtt
     6961 tgttcattat tttgctcagg agctgcggct tgtgcctgct gagaagcctg ttcagctgct
     7021 gcttgctgtc gcgcctgttc tgcttgttgc tgtgcgattg cttcttgcct cgcctgttct
     7081 gcttgttgct gtgcgattgc ttcttgttgc gcttgttctg cttgttgtcg tgcaatttct
     7141 tcttgctttt tccgttcttc ttctgcttct tttgctttga ttgtggaatc aattgcagca
     7201 agacggcttg tcaactcttg tttgttttca ggtagcgctt ggatagctgc tgaagcagtt
     7261 gcatagtttt cttttgtagg atttgcttca gcttgtgaca aagcagattc cgcttctgtc
     7321 actttttttt ctagtgcagc tttttccgct ttttgtttct cttcttcttt tttcttttca
     7381 atttgttctt tcgatgaatc tgccgtactt tcggagatcg tggtactgct gctggaagcc
     7441 tgagctgaac ttgtgctttg agtagtagac gtagtagcac cacacccact caacaaactt
     7501 cctgcgacca aaaacaacgc aactaatttt ttcactaaat ttcctcctta agtaaaaaac
     7561 aaccgttttc aataaatgtc cttttcaatt gtttacttac ttgttataat aattgtgtta
     7621 tgaatagtga ttgaggaaac ttaatctaat cccgtgttga tacacgggat tttttgctat
     7681 agt
//
LOCUS       DAZUMB010000121         7592 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000121 DAZUMB010000000
VERSION     DAZUMB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.124"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..787)
                     /locus_tag="ACG80L_002314"
     CDS             complement(59..787)
                     /locus_tag="ACG80L_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5024522.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="ACG80L_002315"
     CDS             complement(800..2080)
                     /locus_tag="ACG80L_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5024523.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="ACG80L_002316"
     CDS             complement(2221..2823)
                     /locus_tag="ACG80L_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5024524.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="ACG80L_002317"
     CDS             3068..3274
                     /locus_tag="ACG80L_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024525.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="ACG80L_002318"
     CDS             3586..4305
                     /locus_tag="ACG80L_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5024526.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="ACG80L_002319"
     CDS             complement(4386..5069)
                     /locus_tag="ACG80L_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS5024527.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="ACG80L_002320"
     CDS             5287..7473
                     /locus_tag="ACG80L_002320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS5024528.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2720 a         1281 c         1427 g         2164 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa ca
//
LOCUS       DAZUMB010000122         7584 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000122 DAZUMB010000000
VERSION     DAZUMB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.125"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACG80L_002321"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACG80L_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS5024529.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACG80L_002322"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACG80L_002322"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS5024530.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACG80L_002323"
     CDS             complement(2303..2965)
                     /locus_tag="ACG80L_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5024531.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACG80L_002324"
     CDS             3149..4795
                     /locus_tag="ACG80L_002324"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5024532.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACG80L_002325"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACG80L_002325"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5024533.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="ACG80L_002326"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="ACG80L_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2380 a         1380 c         1396 g         2428 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttt
//
LOCUS       DAZUMB010000123         7534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000123 DAZUMB010000000
VERSION     DAZUMB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.126"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG80L_002327"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG80L_002327"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS5024534.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACG80L_002328"
     CDS             complement(2032..3285)
                     /locus_tag="ACG80L_002328"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS5024535.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACG80L_002329"
     CDS             complement(3291..4214)
                     /locus_tag="ACG80L_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS5024536.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACG80L_002330"
     CDS             complement(4435..6927)
                     /locus_tag="ACG80L_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS5024537.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACG80L_002331"
     CDS             complement(6934..7401)
                     /locus_tag="ACG80L_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS5024538.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2075 a         1638 c         1208 g         2613 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAZUMB010000124         7419 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000124 DAZUMB010000000
VERSION     DAZUMB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.127"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..778)
                     /gene="nth"
                     /locus_tag="ACG80L_002332"
     CDS             complement(101..778)
                     /gene="nth"
                     /locus_tag="ACG80L_002332"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS5024539.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(785..1483)
                     /locus_tag="ACG80L_002333"
     CDS             complement(785..1483)
                     /locus_tag="ACG80L_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5024540.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1751..2935
                     /locus_tag="ACG80L_002334"
     CDS             1751..2935
                     /locus_tag="ACG80L_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS5024541.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2941..3507)
                     /locus_tag="ACG80L_002335"
     CDS             complement(2941..3507)
                     /locus_tag="ACG80L_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5024542.1"
                     /translation="MTSFVNPFFSWFQIGFSYKIFLSLLNRKQKNNIMKEKEGVEMDK
                     RQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHIGKIPDPNVTKLANASYM
                     TRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKIRNRHEQLHQKWVQRDAT
                     VFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3610..3897)
                     /locus_tag="ACG80L_002336"
     CDS             complement(3610..3897)
                     /locus_tag="ACG80L_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5024543.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4068..5420)
                     /locus_tag="ACG80L_002337"
     CDS             complement(4068..5420)
                     /locus_tag="ACG80L_002337"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS5024544.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5693..6139)
                     /locus_tag="ACG80L_002338"
     CDS             complement(5693..6139)
                     /locus_tag="ACG80L_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5024545.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6375..7301)
                     /gene="cysK"
                     /locus_tag="ACG80L_002339"
     CDS             complement(6375..7301)
                     /gene="cysK"
                     /locus_tag="ACG80L_002339"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS5024546.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         1996 a         1526 c         1223 g         2674 t
ORIGIN      
        1 atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc cataataaca
       61 agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt
      121 ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt gatagcaaag
      181 gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg
      241 tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt tcttctactt
      301 ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg
      361 tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt gctgtctttc
      421 tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc ccatcgaaac
      481 gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga tacaaaccaa
      541 tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg
      601 gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc gcctgggcac
      661 ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct ccatgtgctt
      721 cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg cttaacatat
      781 tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct tgttgagctg
      841 actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct tcttccactt
      901 gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca acgtaattaa
      961 aactataagc ctgatttaat acggcttccc gcagtgctag cttcaaaatt tccggctgat
     1021 aatggtcttc ttctagccat tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc
     1081 caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt gttttttctt
     1141 cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg gggtaaagat
     1201 tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata aatccatttg
     1261 ttacgagacg atccagtatt tgaaaaatct gatcttgttt gattcccata tccatagcaa
     1321 tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac tgtagaacaa
     1381 ataaaaattc ttcagattgt agaccgatcc tatgataatt tttaagtaaa agattagata
     1441 tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct cctccgctca
     1501 atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc tatttacttc
     1561 actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat gtttttgatg
     1621 cgtatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct tagaatgttg
     1681 acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta ttgcataaag
     1741 gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat acgtctctgt
     1801 cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt taacgctgcc
     1861 ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt ctgtttatgc
     1921 tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt ttgggcgtca
     1981 tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt ttggtttagg
     2041 aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg caggagaaat
     2101 ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa ccaaagtttt
     2161 tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta tagggggtct
     2221 attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta ctttcttaaa
     2281 tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac gttcagtaga
     2341 tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca agatttcatc
     2401 cgttattcct ttattgacag caggttttgc tgtttggttg gcagctggct ctcttcaatc
     2461 tatcttttct caattttcga ttgacgcctt tcagtggaag gctgggttgg ttggtttaag
     2521 cttctctttg attggaatct tggatactgt ctcgcaattg ttcattatgc ctcgattgct
     2581 caaaaaattt tcagaacagc agatcacaag aatagggatg ttcagtgaaa tcctcgccta
     2641 tctattcatc acattatctg gaattcttct actgccgatt ttgtttttat ttgggattat
     2701 ctgttatggt ttcggtgatt ctatttttac tcctgtattc aatggccaac tatccaattc
     2761 tgtctcagat aaccagcaag gtcttgtaat gggcggtacc caaagtatac aatctctatc
     2821 gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct gcagcaccat ttcttccgac
     2881 ggtattcgga tttttgctta tgatttttgc ctattatcac tacaataaaa aatagtagct
     2941 tcatttgcta cttcgtataa attctctgta tcgaccgata aaacgaaata ctgtgtcaaa
     3001 ttcttctttt gacatatttt caaatacagt agcatctcgc tgtacccatt tttgatggag
     3061 ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc aattgaaaat agatttcttt
     3121 tttattctct tcgctttgat aagtgtttat gatccctcta gctatcagtt tttttgttag
     3181 cttactgatg gctcctctag tcatgtaact agcattcgct aattttgtca cgttaggatc
     3241 agggattttc ccgatatgtt caatcacttc tagttcattt agagtaaatc ctgcaaattc
     3301 tttttccatt tctggtttat tacgatgaat aatctcatta gctaaatcca gatattcttc
     3361 tattagcagt tgtcttttat ccatttctac accttctttt tctttcatta tattgttttt
     3421 ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa aacccaatct gaaaccaaga
     3481 aaaaaagggg ttgacaaagc ttgtcataac ccctttttct gattaaaaga tagtaaagag
     3541 acagtcatcg gtaattgtga tgattttttg tcatcactta aggatgagcg gccttttcaa
     3601 aaactgtctt taaagatcat catcagtttc tagcacatat ggctgacctt catatgtgta
     3661 ctctgtattc aatgtgccgt cattttcatc tgagtagatg cgtctactga aaggaaaacg
     3721 atgagaaaca ttatctttgg cgtcatttgc tggcatcgtg taatattcct cttgcgtttc
     3781 attcaactga gagagtactt gatctgccaa ctcatcaata ttgatcgtta atttcttcga
     3841 agcactttta taatcttcta aaaatgcatc ttgttggaca aaatattctt tattcatctt
     3901 ctttctcacc tttcttttca aaatcaaata aagctgcttg ttcttttatg taacagcttt
     3961 atctttgctg cttattgtga caaaaaattc gaagaaagac aactaattgt acttttcaca
     4021 aaaaaagaca gagggtcgtg acaaaattgt gtcacaaccc tttttcttta ttgtgcagtt
     4081 tcgatttcgc ctagcgctgc ttgtgcaagt aggttcaagt aattccatgg gcggtcaaaa
     4141 tgtggttgga agaagaaatc tgaaagtgcg agatcttcta cagtcatttt attttgaaca
     4201 gctaaagaca tggtatttgc tgattgtgta atgtcgtatt tggacatgaa ctgagcacct
     4261 acgatacgat tcgttccttt ttcatagact agctccatca ttactttttc agtagttggc
     4321 atgaactctg gacgataatt atcttcaaat acagtcgctt ggacttctag atcgttcaaa
     4381 gttgcgcttt ctttggtcac acctgtcgaa ccgatcgtcc agccaaatag gtaaagtcct
     4441 gaagttcctt gagtaccacg atacgccatt ttatgatctg tcaagttatt tcccacaagc
     4501 aaaccttgac gaacggcatt tgttgccaaa ggaatatagt tttgcgtgcc acttggattg
     4561 tagttgacta ctgcagagtc tccagctgca aaaatgtctg gattacttgt ctgcatgtat
     4621 tcattcactt tgattgcccc gtttggcagt gtatctactt ttccttcgat cagttttgta
     4681 tttggacgga agcccacaca taaaatgacc atatctgtat caaatgtatc tgttggtgta
     4741 gctacttgtt tcacttttcc ttcttcatct gtgataaatt cagtaacatt ttcgcctagc
     4801 gcaagagtaa caccgcggtc tgtcaattct ttttctaaga tatctgtgaa tggtttatct
     4861 agatatttat tcaagatgcg atctaatcca tcaatcaatg tcacttgttt tccagattcg
     4921 acaaaagctt ctactaattc aataccgata tatccaccac caacaattac tacacgtttt
     4981 gcttctttcg cttgtgcaat aatttcattt gcttgattgt agtttttgca taggagtaca
     5041 tttttcgcat cgattccact aatcggtggg ataataggcc atgaaccagt tgtcatcact
     5101 aatttatcat aagaaaccgt ttctgtttct cctgttttca gatcttttgc cgttactgtt
     5161 ttattttctg tatcgacgtc tgttacatcg tgttccattt taactgttgc tcctaatgaa
     5221 gataattctt caggatttga gtagaacaag cctgctgggt cctttactac tccgccgaca
     5281 taaagtgcga taccgcaaga taagaaagaa atattgtcat ttctttcata tactgtcact
     5341 tctgcttctg gatgattttt taaaatactt tttacagctg cagtacctgc atgagtacag
     5401 ccaattacta ctactttcat ttatggtccc tccgattaac attttgtcct tcgacaaaac
     5461 tactataaca gaaataaaac ggatacaaca gtttaatgct tgtgaattca caaaggtttg
     5521 tttgtagttt cattcctttt caagcataag aacccaatat ctacaagatt cttattttaa
     5581 taatattcac ttttatatct aagattgtga ataaaagaca aaaaaataaa aaagctagcg
     5641 ttttttttac acagctagct ttttttaggt ataacacttg tagaaactgt ttttacattt
     5701 gcgccttttt tgctgactct ttacaatccg gacagagtcc atacaattcc aaccgatgtt
     5761 cattaatctc aaaatcagtc agtctgcttg cagccatttc tatatcttca agccctgggt
     5821 aatgaaaatc aacaatctta ccgcagtttt cacaaattac atgataatga cgctttgaac
     5881 tgaaatcaaa tcggctagaa gcatcgccgt acttcatttc ttgaacaaat ccaatcgaag
     5941 taaataagcg aaggttattg taaacagtcg caacactcat atttggaaac cgatgttcaa
     6001 gtgctttata aatctcatca gccgttggat gggaatgatg ttcaatcaaa tattctaaaa
     6061 ccgcgtaccg ttgaggtgtg attcgaatgt tctctttttt taacttttcg ataacctctt
     6121 cgactaactt attttccatg taaacacccc acgttgttat tctcgtctta tcatactatt
     6181 tttacatttt aaaatccact taacatgaaa agaaaattca attttattgt attttaatga
     6241 aaaaagacgt aaaacagtcg cgttcactat tctacgtctc tattcaaata atttttctca
     6301 tcataaattt ttgtgtgttc ttttcatctt taaaagtttt ttacataaac ctatctggag
     6361 aaaaaccaag tgccttatat ctcttggtaa agggctgtag ataaataacg ttcaccattg
     6421 tcgggaacca cagctaatac cttcttgcct tttcctaatt cttttgcgac ttttagcgcg
     6481 gcagcaattg cagcgccaga tgagatgcct actaaaatcc cttcttttcg cccaacttct
     6541 ctagctgttt ccatcgcttc gtctccacta atagaaagga ctttatcata cacagaagta
     6601 tccagtgttt taggaacaaa acctgtaccg attccttgga ttttgtgagg tcccgcttct
     6661 ttcccttcta aaatagcaga ttctgctggt tctaccccga ttaattcgat tttcggatac
     6721 acacgtttta attctcggcc agcgccagtg atcgttcctc ctgtaccgat acctgcaaca
     6781 aacgcgtcca agccatttac tccaaatgct tgatgtatct caggaccagt cgtcttctca
     6841 tgcacaagag gatttgcttc attttcaaat tgtaatggta aaaagtagcc gttttctttt
     6901 gccaaacgtt ctgcttctct gattgatcca gaaataccat ccgcaccagg agtcaaaatc
     6961 aactctgcgc cgtacccttt catcaataaa cgtctttcga tactcatcgt ctcaggcatc
     7021 acgatgatta ctttatatcc ttttgcaact cctaccatcg acaatccgat tcctgtattt
     7081 ccagaagtag gttcaatgat agtatcacct ggtttcagta gaccatcgtg ttctgctttt
     7141 tctatcatac ttaaagcaat acgatctttt acacttcctc ctggattaaa aaactcaagc
     7201 tttacaaaaa catctgctga atcttctggt acgattttat ttaatttgac gattggcgtt
     7261 ttgccaatca attcggtgac tgagttatag atagctgtca tcttcaattc ctccttgtct
     7321 ttgctcttct tacttatcat aatacaatct gttcatttat ggcgagtgat tcgtctttga
     7381 ctagcaaaaa aaagacaaag atataccata tatggtaat
//
LOCUS       DAZUMB010000125         7285 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000125 DAZUMB010000000
VERSION     DAZUMB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.128"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..2681
                     /gene="clpB"
                     /locus_tag="ACG80L_002340"
     CDS             72..2681
                     /gene="clpB"
                     /locus_tag="ACG80L_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS5024547.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2832..3518)
                     /locus_tag="ACG80L_002341"
     CDS             complement(2832..3518)
                     /locus_tag="ACG80L_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024548.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3496..3990)
                     /locus_tag="ACG80L_002342"
     CDS             complement(3496..3990)
                     /locus_tag="ACG80L_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5024549.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4010..5230)
                     /locus_tag="ACG80L_002343"
     CDS             complement(4010..5230)
                     /locus_tag="ACG80L_002343"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5024550.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5377..7212
                     /gene="lepA"
                     /locus_tag="ACG80L_002344"
     CDS             5377..7212
                     /gene="lepA"
                     /locus_tag="ACG80L_002344"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS5024551.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2395 a         1394 c         1498 g         1998 t
ORIGIN      
        1 agcgatataa tagtttttgt aaagatcaaa gaaataagtg atcttacctt taattaggag
       61 ggtatacctg tatgaatatt gagaaaatga ctacgacgct tcaagaagcg attgctgaag
      121 ctcaacagat tgcggtcact cgaaaacatc aagatatcga cattgcccat gtatggaaaa
      181 tctttttaca atctaatcat tttggtcgaa atttctatac ggatgccgga ttggatgtcg
      241 aatcttttga acatgaaatt gatcgtctac tggatgagta tccagttgtt tctggtggaa
      301 acgtccaata tggacaaaac ctcagccaga atttctttcg attattgaat gaagcagacc
      361 aaatacgtga aagcttcggc gacgagtttc tttcaacgga agtcgttatt ttagcgttga
      421 tgaaattaaa aaattatccg ttaactgttt atttgaataa aaacggatta tctgaaaaag
      481 aattgcgtaa aaacatcgaa gagatgagag gaggagaaag agtgacctca caaaaccaag
      541 aagaacaata caaagcatta gaaaaatacg gcgttgattt agtacagcaa gtcaaaagcg
      601 gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc cgtattcttt
      661 cacgaaaaac aaaaaataat cctgttctga taggagaacc cggtgtaggg aaaacggcaa
      721 tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat ttaaaagata
      781 aaacgatttt ttcattagat atgggtgcat tgatcgctgg agcaaaattc cgtggtgaat
      841 ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga cgtatcctct
      901 tgttcatcga tgaaatccac aatatcgtag gggcaggaaa aacagaagga agtatggatg
      961 ccggaaatct gttgaaacca atgcttgctc gcggtgaatt acatttgatc ggtgccacaa
     1021 ctttagatga gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt cgctttcaaa
     1081 aggtattagt caaagaacca acagtggaag atacgatcag tattctgcgt ggattaaaag
     1141 aacgttttga gatccaccat ggtgttaaca tccatgacaa cgcattagta gcagcagcta
     1201 ctttatctga ccgttatatt actgatcggt tcttaccgga caaagcaatc gatttgatcg
     1261 atgaagccag tgcaacgatt cgggtagaaa tgaactctat gccaacggaa ttagatcaag
     1321 ttactagacg tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa gaatcagatg
     1381 atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt agctgaactt cgtgaagaag
     1441 caaatgcgat gaagatgcag tgggaaacag aaaaagaaga agtcaattct gtttcagcaa
     1501 aacgtgctga aatcgataaa gcaaaacacg aattagaaga tgcagaaaat aactatgatt
     1561 tagaacgcgc tgctgtattg cgtcatggta cgatacctca attagaaaaa gaattgaagg
     1621 aattagaagc taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc gttaccgaaa
     1681 atgagattgc tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa cttgtagaag
     1741 gtgaacgaga aaaactaatt aaattgaatg aaacacttca taaacgagtg attggtcaag
     1801 atgaagcagt tgatgcggtc agtgacgcgg taattcgttc acgggctgga ttgcaagacc
     1861 caaatcgtcc tctaggttct ttccttttcc taggaccaac tggtgtcgga aaaaccgaat
     1921 tagcgaaagc gttagctgag aacttgtttg actcagaaga tcatatggta cggatcgata
     1981 tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt cggagctcct ccaggctatg
     2041 taggttacga agaaggtggt caattaacgg aagctgtcag acgaaatcct tatacaatcg
     2101 tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg ttacaagtcc
     2161 tagatgacgg acgcttgact gattcaaaag ggcgagtagt agatttcaaa aatacggttc
     2221 tgatcatgac cagcaatatc ggttcacagt tactgctgga aggcgtgact gctgatggaa
     2281 cgattccaga agcagtggct gaacaagtca acacgctatt acgtggaaac ttcaaaccag
     2341 aattcttgaa ccgaatcgac gacacgattt tatttacacc attgagccta gacaacgtga
     2401 aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg tctggaacat caagaaatcc
     2461 tgctgacaat cagtgatgaa gccaaaacgt ggattgctga aaatgcttat gaaccagcat
     2521 atggtgcaag accattgaaa cgatttatca ctaaggaagt agaaacacca ctagctaaag
     2581 aaatcgttgc aggacatgtt atgcctaaat caaaagtaac gattacttta cttgatggac
     2641 aacttcattt caaaacagaa gagctagaag aaatcgtcta atcttccatt gagactataa
     2701 aagaaaaaag gggtctgaca caactagtgt cagacctcct tttttcaaat aaacagtgtt
     2761 ccagaagtag tttcttcgaa aaataagccg aaatttcttg aagctgcccg cttctgtcac
     2821 aacctctttt attaggtatt tttcactttt tctaatttat aactttcaaa gtaacgctca
     2881 tcttttttac tttgagacca tgcaaacaat cccattaagg cagcacctat cgtatttgtc
     2941 agcaagtcgc ccatcgtatc catcaatgct gctcgtccga caaacagtgt gccatcagaa
     3001 gcagcaaaac gttgaagatt catgcctaag aactggtcgc ataagaattc ccagaactcc
     3061 caaaacacgc cgcaaagtcc agcaaacgca aagccgaata agaggaacaa ccaaggactg
     3121 gtttttgata tctcagcatc tttcatcaag taaccaatca atccatagcc aagagctgtc
     3181 aggaccattg gactcaccgc atgtaagatt ttgtcccaaa aagagatgat actgatcaaa
     3241 tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa caaaacgatt
     3301 gctgctggta ttttaagttt gaaaactttc aaaatgactt gtggcatgaa aatcaatgca
     3361 atccctaata aagattccac gataaatgtg attccttgag aagtcgaata tttttgtgtg
     3421 ataaattcat aaaaattata gcagacaacg aatacaccaa aagcaagtaa agctatttgc
     3481 acctttttga tttctttaat cactgaaagt ccctccatct aaaaatcttt ttacttctga
     3541 aagcaactca atttctgaag aaaccacttt tccgttcaat ttgatcaaac ctattgtata
     3601 taagttcagg tagtgaaatt gattttcagc tgtttcttgt agtgcagcga tttttttcgg
     3661 gtgggttccg ccttgctgtc gagtatcagt atataagccg atcactggga tgcctttcgc
     3721 ataagccaca ccgatttccg atgctacccc ggcatcgatc gtcagcccat ctagtaaagc
     3781 aatcattaaa tcactagcca acactttttc tgtgtcagca agagcaatca ttcgactgtc
     3841 tgcataagca gttttgtcat tgattgcttc attttcttga ggaagataga tcgaaagctc
     3901 agaagagagc tctctgattt ctttaactaa ctgttggtta tataacaaat cacttttagc
     3961 aaacattggt gcggcaaaat aaattttcat ctaaacatca tcctttttat caaaaataat
     4021 cgctaaagta agcttccatg tttggaatga tacgctctag tgacttcagt gtttcttcat
     4081 ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg gcgataattc atcatcaagc
     4141 aagtcaatgt ttggatatcc agtcgaacgg attttcctat aggttcattt aagacacgga
     4201 cttgtccctg ttcatcccat gttaaagcga agacaccatt attccaagga gcgacaggat
     4261 cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt tgaaggaatt
     4321 cttttacatc aacgatccga gccataaaat atggttcgat ctgctctttg atctgactat
     4381 cttcaatcaa aaatgctaaa ggctcatttt tataaatatc tccatgtacc cagtagatca
     4441 tggaaaaatg tgcggaaata aagttccata agccatttcg agcttcttga tcaagataga
     4501 acatttcttt tacgtgaaat atttcttcag ctacccagta gaacagaaca cctcttggtt
     4561 catggttgct atcgtagtag acagcggctg tgcgctcatc ttcattttcg aaacgccaat
     4621 actcttccca atgaaaatca ctgcggaata aagcaccatg gttttgttgg gcaaatttgt
     4681 cataaacgga aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata ccacttactg
     4741 ggatttgttt aggtagctgt gtatctctga ttttaaagga aagtttatca gacatgattt
     4801 cccatccttt acgccgatag taggggatac tgtaaggata caaataagag atccattgct
     4861 tattcttgcg catattatcc aatgcaacga taattagatc ttgcatcaat ccatggttag
     4921 catattctgg ataagttcca actccagtca caccacccat tttataacgt gcaccgtgaa
     4981 tattgacttc acaaggatag atagcgattt gagagatcag tttattttcg tggaaccatc
     5041 cgaacacttt cgatacttcc aaaatcggtt ttttggaacg gataaaagct cgtttattct
     5101 caaacccgct ttcctcgatg tctgcttcag tcacttggaa cacataggaa agcaattcat
     5161 tgaattgatc gatatgttct tgagtgaccg gttttaattc aagattttct ctaaaatctt
     5221 tttgatccat tttttcatct ccatttatta gcttgataac agtatagcaa aattctacaa
     5281 aaaaaacttc tatagctgac taattaaaaa ctctttaaat tactgctttt cgttggtata
     5341 atggaaagtg ctgattatat gaaaagagga gacgctatgg atataaagaa aatgaaagaa
     5401 cgccaagagc agattcgtaa tttttcgatc atcgctcata tcgaccatgg gaaatctaca
     5461 ttggccgatc gtattttaga aatgacgcac actgtgacgt ctcgcgaaat gcaagatcag
     5521 ctgttagact cgatggattt ggaacgtgaa cgtggaatca cgattaaatt aaatgctgta
     5581 gaactcaatt acacagcgaa aaatggagaa acatatatct ttcacttgat tgacacccca
     5641 gggcacgtcg acttcaccta tgaagtctct cgaagccttg ctgcctgtga gggtgccgtt
     5701 ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat tggccaatgt ttatttagcg
     5761 ttagacaatg atttggagat cctgccagtc atcaataaaa tcgacctacc cgcagcagat
     5821 cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag tgaagctgta
     5881 ttagccagtg ccaaatcagg gattgggatt gaagatattc tcgagcagat cgtggaatat
     5941 gttcctgcac caagtggaga cttagatgca ccgctaaaag ctttgatctt tgactctatt
     6001 tatgatagtt accgcggtgt tgttctaaat gtccggatca cggacggtat ggtcaaaccc
     6061 ggagacaaaa tcaaactgat gagtaatggc aaaacatttg atgttacaga ggtcggggtc
     6121 ttctcgccaa aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg ttatatcaca
     6181 gcaagtatca aaactgtcca agatacacga gtaggggata cagtaacgtt agcagacaat
     6241 cctgccgctg aagctttacc aggttaccga aaaatgaatc cgatggttta ttgtggtctt
     6301 tatccaattg atacttctcg ttacaacgat ttgcgggaag ctttagaaaa actgcaattg
     6361 aatgatgctg ctttacaatt cgaaccagaa acctctcaag cattaggttt tgggttccgc
     6421 tgtggattcc tcggacttct tcatatggat gtcgtccaag aacgcttgga acgggaattc
     6481 aatctagaat tgatcacgac tgcaccatcc gttatttacc atgttaataa aacggatggt
     6541 acgactgcta cagtagataa cccggcagat tttcctgaac ctgtaacgat tcaggatgta
     6601 gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg tgcagtaatg
     6661 gaactttctc aacgaaaacg gggagaattc attacgatgg attatttaga tgattaccga
     6721 gtaaatgtcg tctataatat tccgctttct gaaattgtct ttgacttttt cgacaaattg
     6781 aaatcaagca cgaaaggcta tgcctcattg gattatgaaa tgtctggtta tcaaaaaagc
     6841 aagttagtga agatggacat cttgctgaat ggagaaaaag tcgatgcatt aagctttatc
     6901 gttcaccgag actttgccta tgaacgtgga aaagcaatcg ttgaaaaact gaaaaaacta
     6961 atcccacgcc aacaatttga ggtgccaatc caagcagcta ttggacaaaa aatcgttgcc
     7021 cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg tggagatgtt
     7081 tctcgtaaac gtaaactctt agagaaacaa aaagaaggga agaagcggat gaaacaaatc
     7141 ggctccgtcg aagttccgca agaagccttc atggccgttc tgaaaatgga cgaggacgaa
     7201 ccgaagaaat agcaagggtt tgtttgaaaa cagtgtaaaa aataaaccat gtttacatgg
     7261 atttgccacc ggtttgccac cggtg
//
LOCUS       DAZUMB010000126         7048 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000126 DAZUMB010000000
VERSION     DAZUMB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.129"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2521
                     /locus_tag="ACG80L_002345"
     CDS             <1..2521
                     /locus_tag="ACG80L_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS5024552.1"
                     /translation="EKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDI
                     DPKRKEGSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNH
                     ETILTIQNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSS
                     FLSIPDNIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPE
                     LTYTIKEGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQ
                     DFVKKYPLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIR
                     KNIEQKVITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQ
                     IQRENAPIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFD
                     ALVHKYVLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFI
                     ASLFGNPTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGE
                     ERLRLDSNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANH
                     AEKVKSLSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNN
                     LALIYSGALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMN
                     SHKFLTNSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQL
                     LDSGNMCAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2703..2942
                     /locus_tag="ACG80L_002346"
     CDS             2703..2942
                     /locus_tag="ACG80L_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024553.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2958..4331
                     /locus_tag="ACG80L_002347"
     CDS             2958..4331
                     /locus_tag="ACG80L_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5024554.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4335..5969
                     /locus_tag="ACG80L_002348"
     CDS             4335..5969
                     /locus_tag="ACG80L_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5024555.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6132..6338
                     /locus_tag="ACG80L_002349"
     CDS             6132..6338
                     /locus_tag="ACG80L_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024556.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTG"
     gene            6407..7048
                     /locus_tag="ACG80L_002350"
                     /pseudo
     CDS             6407..7048
                     /locus_tag="ACG80L_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT         2514 a         1156 c         1416 g         1962 t
ORIGIN      
        1 agaaaaacag aaattgtctg aaggtacagt aaacataact tataatccta ttaaacaaga
       61 ggtgattaca caaaaaaagc attctgtgag agacaatgat attgaaaaaa ttcggtttgt
      121 atttattgat attgacccta aaaggaaaga gggaagtgca acagatagcg aaaagaaaaa
      181 ggcagaaaat gttatggaac aagtagaacg ttatttgaaa gaaaagagaa tagaatcatt
      241 tgtaaaagtt gatagtggga atggctacca tatttttctt cccataaatg aacaaccaaa
      301 taatcacgag acgattctta ctattcagaa ttttcttcaa ttacttcacc gaagatttgg
      361 agtagaagat gaagttgata ttgatacttc tgtttataat ccttcaagat tatgcaaatt
      421 agttggtaca ccagcggtca aaggaaagtc cacaaaagag cgtccgcatc gtttatctag
      481 tttcctttct attcccgata acatagatag aagtaattgt tttaaaattc ttgaacagat
      541 agttgaagaa aattccatga tgtcaacctc tagagtaaaa agccatccat cttttagaga
      601 agtgttacct tttattcaag cagactataa acaatggtta gctcactatc ctgaattaac
      661 ttataccata aaagagggcg atcataaagg agttacctta cttatatttg acgaatgtcc
      721 attaagaaaa cacacgaata attctaatgg ttcttgtttg tctgttacgg ctgataataa
      781 gattcgattt cattgtctgc atgcttcgga taaaaataaa gatattcaag actttgtaaa
      841 aaaatatccg ttgccaaaac ctgtaaatcc ttctgtagaa tcattagaat ccaatcaaga
      901 atattatttt gaccagttta aactgaatca agaaggagta tttttggtga aagaaggtca
      961 agaagaaaaa gttgcttgtc cgatgtttat taagcaaata cgcaaaaata tcgaacaaaa
     1021 agtcattaca cttgttcttg cctattatgt cgatcattgt tgggatgaaa tagaaataga
     1081 aggtggatgt ttacaaacta ataattttaa acaattagca aaacatggct taatcattaa
     1141 agctagaaga gaagctgaag tcgttgactt tttacagatt caacgggaga atgctccaat
     1201 cttttttgaa catgaatctc ttggctggaa aaaagaaaat gaagcaatga tttttcagtt
     1261 acaagattct tttggacgtg aaattccttc tgtattaact aaaaactcat gctaccaatt
     1321 aacaactaat ggtacagaag cggaatttga cgcattggtt cataagtatg ttttaggaac
     1381 gaacttagaa cttgcttggt cgattggtat tgcttctatt gtgttaggct atttaaacgt
     1441 tacgaaggct aatacttttt tgagtttact aattaaccta cagggcaaga gcacaacggg
     1501 taaaaccact acgttacact ttattgcctc tttatttgga aatccaacta cggttcttag
     1561 aaatatgaat gctaccaata aagccattat aaagctagca tcaaacaatc agggaggtgt
     1621 tccactaata ctagatgagt taggagcagc aactcaaaat aactttagct cgttgatcta
     1681 tcaattagct tcaggagaag aaaggctaag attagattct aattgtcagc ttatgccaca
     1741 agagactttt tctgttttga cccttacctc ttctgaagag cgtttggaaa attatttcaa
     1801 tgagaaatta actggtttga aagttcgata tatcgaattt tctgatatca agtttacaaa
     1861 ggatgcgaat cacgcagaga aagttaaaag tctttcttct aatacttttg gttggggtat
     1921 aaagttattt cttttgaaac tctttgaaca agggattaca aaaattgaag caacttttgt
     1981 cgaagctcaa aaaaaactat gggctgtttt acctgaacat tctttgaagc aacgcattgg
     2041 taataatcta gctttgattt atagtggagc cctcctaatt caagatatac tgggctatcc
     2101 tttacgatta gagcatatac agacggtgct tattaattcg tacgaacagt taattgtaga
     2161 tactagttta ggaagtgagc aaacggtatt tgaccgagtg aaaacattgt tggtgatgaa
     2221 tagccataaa tttttaacaa acagtgacaa caaacatacg cccagtacga tttggggaaa
     2281 agtatcaatt caaaaaaatg gggatattaa ggtaaatatt tttcctatcc cgtttgagga
     2341 cttgttaagg cgagaatttc aagtaaagga tctaacctat ctttttaggc aattgcttga
     2401 ttcgggtaat atgtgtgcag aaaaagggag aagaacgaaa cgtattcaca tgaatcgtca
     2461 agttcttcca acatacgaga ttctattacc ttcttcactc aaatcgtatt ttcgattgta
     2521 atttagaata aaccaaagta tgaaagtgag gcaccatgtt atggaaatat ttcttattga
     2581 tttagcaaaa caactgatta cttatctgta tgatagcgag caaacccaaa cggaagtaaa
     2641 tgaagcttga tctatgcttc atttacttca ttcaatcaaa catgaaagaa aggaaaattc
     2701 taatgactaa aaataaaatg acactaaaag cagaggttct tctgtatatt caagaacatt
     2761 tttcaaatca agcctttttt acaaaaccaa tttacttgga tttcgaaata agaggggtat
     2821 ctgctggatc tatcgggggt actcttcaag cgttgaagaa cgaaggatat ttagaaaatc
     2881 actttgtgca acgttcgttc aatagaagag tcgtgaaaaa atggtattta gttcattcat
     2941 agagagggga atcaagcatg cgtgtggctt gttatgatcg aacgtctgtt tatgaagaag
     3001 cgaaaattag taatgacgct caatatgatg cagtgaaaga gtatgtttta gagaaatatg
     3061 aacaaattcc ccaacgattt tcagacaaag gagaatcagg ggaaaaagcg gagcgtccag
     3121 cttttcgaca gcttagagct tgggtttgtg cgtcagaaga agagggggtg atctatgttt
     3181 ggcgttatga tcgattggca cgtcaagcca aaatagcctt agaatttatt gagttgtgcc
     3241 aacaacatca ggttcaagtg tattcgattt ctgaacctct tcctgcttct ttctcacacg
     3301 cgtcaacgaa taaactattt gtgcaattat tgttcacttt tgcagaacaa caacgaaacg
     3361 tcattattga gaatgttcgt gccggacttt cctataaaaa gcaacaacgg caatatttgt
     3421 cttctgcggt accatatggg tatcgtttag tgaatgggaa aatccaacaa gaagtacacg
     3481 aagcaaggat tgttcgctat atttttcaac tttatttgac caaaaaatat ggctacaaaa
     3541 agctttgtca acgattgacg caacaaaagc ttttcttccg cgaacgtcct tttcagccct
     3601 atcatattta tagtatccta aaaaatccat tgtactatgg cgaaatcaaa ggaggatctt
     3661 tggggaagta tctaggaacc tttgaaccga ttctctcgaa agcaaccttt ttacaagccc
     3721 aagagattcg ccaatcaagg tgtacagcta aaaaagatac gtatctgtac ttattacgtc
     3781 aaaaaattcg gtgtcccttc tgtggtcgtc atctgagttc gaagtatcaa tggaatacaa
     3841 agaagacgaa gacgcttcat tattatcatt gtaccgaccg aagctgccaa ggaatttttc
     3901 ttcgggcaga aagggttgag tcagaagtat tggaaaagat acaggctttt tttcaatacg
     3961 aaggcgtcta tcaagccttg attgcccaat taacgcaaca attagccgaa aagcgaaaag
     4021 atgaaaaacg caaagtaatc caacaaaaga aacaagaagc acaatggatt aaggcatttg
     4081 aagagggcaa ttgttcactg gaagaactca agcaacaact gcaagctctt caaaagccga
     4141 tgtctacttc ttcgtttctt tcactccaac agtgtgaagc aaaagtgaaa gaatggcttc
     4201 aactaaaaga cctctctagt cgacaattat tatgtgacta tgtttgtcgg attgaggtaa
     4261 caaaggataa aacagtttgt gggatttact tccgacaact gacacaaaat attttagaaa
     4321 ggaacgaatg atctatggaa atgatacaaa ccaaacgagt cgcattgtat attcgcgtga
     4381 gtacacaaga acaagcttta gaaggacata gtattcaagc acaagaacaa aatggacgac
     4441 agtttgccga acggatgggc tatgaagtgg tagaagttta tgccgatgaa ggaatcagtg
     4501 ggaaatccac aaaaaatcgg ttcgcctatc aacgaatgat gaacgatgct cggaaccatc
     4561 gttttgatct actagtgatt tggaaattga cccgtctcgg acgcaatatg ttagatgtct
     4621 tacaaacagt cgaggaatta ttgcgatatg acgtaggatt acattcgatc tcggaacaat
     4681 ttgacgtgac cacttcgaca ggtaaattga tgttacaact ccttgggagt ttcggtgaat
     4741 ttgaacgcaa ccaaattgcg gagaatgttc aaatgtcaat gcaaagctta gtgaaagata
     4801 aaaagcgtta tgcaggaggc cgacgtttag gctatgtcag tggtattgac acgaatggtc
     4861 aaaaacaatt ggtcattgaa ccagaagagg cgaaaattgt tcgtctgatc tatgcaaaat
     4921 atttaggcgg agcagggtat cgagcgattg ccaatttttt gaatcgtcaa ggatatcaaa
     4981 cagtcaaaaa gaatgctttt tctaccacgg cagtcaaaga tatcttacgc aataaaattt
     5041 atgcagggta tttagaatat gcgcgctaca tcaattggga aaccaaacga cggaagggga
     5101 agaatccgag accgatttta gtgaaaggaa cccatgaacc gattatttcg gaagaagatt
     5161 atcaaaaagt acaagaacgc cttgctttag aaggaaaaca acccaaatgg aaccatacag
     5221 gtgaaaatgt attgacaggc ttgttacgtt gtccagaatg tggcgcaccg atggcagcga
     5281 gtaatgtcac aaatacccta aaggatggca caaagaaacg gattcgatat tattcttgtt
     5341 cagtctttcg gaataaagga gcaagtgtgt gtcatgccaa ttcgattcgt gccgaacaag
     5401 cggaaacatt tgtcgccgac cgattaaagg agatcgttca gctaccagaa gttcttcccc
     5461 gattggtggc agcgttaaat gaagaaatag tgcgacaaag ccaaccattg gaacaagaac
     5521 tcgtggtgtt actggaacga aaggaagaat tgaagactaa aattgaaaaa tgggaagctg
     5581 ctttggaaga tagtcctgaa ttgtttccga tgttaaaaga ccgcttagac gaattgactg
     5641 aaaaacgacg acagttgcac attcgagaaa atgaaatctt agggatcttt caacaaggag
     5701 agccgattca agtcaaagat gtccaacgga tattgactaa tttggatcgt tttcttgccc
     5761 agagtgagaa gaagcaaatc aaagccttgt atcgtacctt tatagagaaa ataacctttg
     5821 atcctaacca taaggagatg ttggaaatta cgatgaaatt cacgcatgcc gtggtacaac
     5881 aattgaatga gcaatatcaa atagccgtat ccaaaacaaa ggatacggct atttttgtgt
     5941 taaaaacacc ctttacgtta acgatctagt ctaggttata tcaaacaacg ttagaggggc
     6001 gctaacgctt ctctagcgat ttgaaatcgt tagagaagta gttagggagg tccttatcat
     6061 aaatagggat atttattaat gaatatttga aatataattg tatataagaa ttaaaaagag
     6121 tggggataga tatgaaaaag atgggtaagt gtattttatg tgaaaaatac actgaactta
     6181 ctaaagaaca tgtaccacct aaaaaatcag ggaatactgg aggtaaaaag accagaactg
     6241 gtaatttaga tgatttcctt aagagcgatt ttacaaaagg tgattttccg aaggggatta
     6301 aaaggaaacc tcaaggaaat gtttattata ctggataggc tacaattaac tagacagaaa
     6361 aattaaggtg tgtagactag aagaaaacat accaggagga atttttatgt ctaagagaac
     6421 acgaagaact ttttcacaag aattcaagca acaaatcgtc aatctttact tagctggaaa
     6481 gccacgtgta gaaatcattc gagaatatga actaacagct tcagcatttg acaaatgggt
     6541 aaagcaatct aaaacgagtg gttcattcaa agaaaaagat aatcttacgc ctgaacaaaa
     6601 agaattgtta gaactacgta aaagaaacca gcaattagaa atggaaaatg atattttaaa
     6661 gcaagcagcg ctgatattcg gacgaagaga caagtaatcg atgcgaataa gcatctttac
     6721 cctatatcag cgatgtgcag aatattaggt ctatcacgtc agtcctatta ttatcaatca
     6781 aaaccaaaga aagacgaatc agaacttgaa gaagtagtcg ctgaagaatt tatccgcagc
     6841 cgaaaggcct acggctcaag aaaaataaaa aaagccttat caaaacgagg cattcagatc
     6901 agccgacgaa aaattagtag aatcatgaaa aatagaggat taaaatcgag ctatactgtt
     6961 gcttatttta aagtacatca ttctacttgc aatgaagcca aaacgacaaa cgtattgaat
     7021 cgtaaattct taagagacaa cccattag
//
LOCUS       DAZUMB010000127         6969 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000127 DAZUMB010000000
VERSION     DAZUMB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.130"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..1259
                     /locus_tag="ACG80L_002351"
     CDS             159..1259
                     /locus_tag="ACG80L_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain"
                     /protein_id="HFS5024557.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSIT
                     SILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYVYTVKKNTNG
                     EYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVS
                     SFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWC
                     AAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPK
                     KVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPW
                     YNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1232..1675
                     /locus_tag="ACG80L_002352"
     CDS             1232..1675
                     /locus_tag="ACG80L_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024558.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2798..3730
                     /locus_tag="ACG80L_002353"
     CDS             2798..3730
                     /locus_tag="ACG80L_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5024559.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            3747..4262
                     /locus_tag="ACG80L_002354"
     CDS             3747..4262
                     /locus_tag="ACG80L_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5024560.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4558..4839
                     /locus_tag="ACG80L_002355"
     CDS             4558..4839
                     /locus_tag="ACG80L_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024561.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            4841..5257
                     /locus_tag="ACG80L_002356"
     CDS             4841..5257
                     /locus_tag="ACG80L_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024562.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
     gene            5959..6183
                     /locus_tag="ACG80L_002357"
     CDS             5959..6183
                     /locus_tag="ACG80L_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5024563.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            6203..6490
                     /locus_tag="ACG80L_002358"
     CDS             6203..6490
                     /locus_tag="ACG80L_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5024564.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT         2652 a         1094 c         1165 g         2058 t
ORIGIN      
        1 attatgttcc caaaacaata ttttatgaac tataacaaaa atttgttatc gcatattgta
       61 tattttaatt ataggtgtat atcacttaat tttaacacaa aggcgtgatt attttgaaac
      121 ccaaaaataa aatttatcta ggtctcctct tttccttgat gattggaatt tttagtatag
      181 gaattacaaa tacctatgct tcagaaaatt ctaatttcaa tatcaactcc caaccaattg
      241 aagaacaagc aattaatttt gcgaaaactg attttgaaca ctccataact tcaattcttt
      301 taatggaaaa taacactgaa actgatatta actatactct tggttcgcca tttaaaatgc
      361 tcgatggaga aaatacggca aatagttcta ttttctttcc agtattttca gacgctgata
      421 atacgattaa atatgtttat accgtcaaaa aaaatacaaa tggagaatat tcaggaagta
      481 tttcacaatt tttagcaaaa gaacttcaaa atctagtaga tagcaatata acagatacaa
      541 ccttattttt tcaagataat agcatttatt attccgataa tggccaaatt aaactacttt
      601 ggaaatcacc tttaagtaca gaaaaaaatg gtgtctcttc ctttacagaa aaaaatgatc
      661 ttatatccgt aaatataaaa aataaaatta aaaatttctc taaaaaacaa aattttcctg
      721 cggttaaacg tgtaaaaagt gtctcctccg gagataactt tattatgatt aattggcgta
      781 tagcagaaac ccaaacaagt tttccgtggt gcgctgctta cgtagaagct gctatcttat
      841 gtaataaaac agatgtacgc gctacttcag ctagagccat tatgcattat acctatccta
      901 atttaaacaa cagtcaaatt gaatcaaaaa gtcttaatca agatcaaata ataaaatatg
      961 caaattactg tggtgtttat cctaaaaaag tagcacgaat actaaacctt tcagaggtcc
     1021 aaacacaact aagaaaagga aatgccattt atatgggaac tgctggttat ggaaattata
     1081 gtaacagccg tcatgcactt gcattatttg ggtgggtcaa tcaaggtggt tatcaaactt
     1141 actatgtgtg gaatccttgg tataactacc caactgtaat aagtgcaaat gcagggccag
     1201 ttacaattcc tgtacctgga ggtggtttta cttgggacaa taccattaca aattggtaaa
     1261 taaacccatt gttttagtta tgggtctagt gttagttctt ttcttaacta gttgttcaaa
     1321 aaaacaagaa atacaaaaga ctgagctgat tccttttcta ggaacttggc aaactaaaga
     1381 tcactcagaa cgtctagtaa tcactaattt aaatacttca aaaaatatag aacaatatac
     1441 catcttagta aaagagaaca atcataaaat tcaaatcatt gataaaaatt taaatataaa
     1501 tgaacaagtt ttcatttaca aagcaacaaa taatttacta acactgcgct ctaataaaaa
     1561 acaattagat tacactttta gtttggattc tccaaaaaag ctcgtattta ttttgacaac
     1621 gaatgatggg acagaaggga cagctaaacc aattgaattt tttaaagctg attgatgaat
     1681 agttgtttga tattacaaaa caataatttc accgaaggtg aaacaagcga aactttacaa
     1741 aaagtggcta gcatttgcta gccttttttg tattgcggag cgtgagtttt gagggggatt
     1801 caagggggag tttctcccct ttgacaagcg gttatttgca ccaaatacga aggtatttgg
     1861 ctagcaaata tagagcttgc caaacccttt agtgtgtttt gtagaaaggg accttatagt
     1921 cactttcaca ttacaaacga tacgtcctat ggacgtatca aaagggtggg acttatgagc
     1981 aaattaaagc aaaatttggc tagcaatcct tctaaaaaat acacctattg atcagagtca
     2041 aaataaatca gttttcgagt gagcgaatcc gaatttgcaa atctgaagct ttgcaaatga
     2101 gtgtgccggc ttccgtaaaa gtctaggtaa cattagtcga tatatacata aggctctaat
     2161 ttgcgtttta aggcgtttta aacgttctgg tatgtaattt ataccaaagc gactttaaaa
     2221 cgccttaaaa cgaattttat aggtcttgta ggacgtttca gggttgttag ggcttgccct
     2281 gaccgcctgt aaggcgcttt aaaaacaaga taagggattt tatctagact tgtcttaaaa
     2341 cgccgtagat gagcgctcag agcttcttaa aggactcctt acgaactgtt tttaagcaaa
     2401 ggagccttta ggcgactgcg cagttcaatg tgagagcgta gcgatcacac ctttaaagcg
     2461 accgtaggga gcagattccc agtttaggaa atttcgcgaa cctgtttgga gaaaaaaagt
     2521 aggaaatttt taggcgggaa attttgcccc cctcttttat aatctctttt ataacctctt
     2581 tttaaacctc tttaccgtcg gctgtatcct tactctccca aggcctgtag cttttttagg
     2641 gcatgaaatt atctgtttat aggcatgaaa ttatctgttt ataggcatga aattatctgt
     2701 ttataggcat gaaattatct gttttaggta ttgttttaag acataatata ccttaaaatt
     2761 atgtcaataa aattagttag actggagtat caaataaatg gcaaatgaaa tcgtaaaata
     2821 taaaccagaa ttaaatacca ttcctttaag gaaatttact ccaatagaaa tgaacttgtt
     2881 tttttctatt gtttctagaa tgcgtgataa aggtaatcaa aaaatacgct tttatttcga
     2941 tcagcttaaa gatttaagtg cttataagca aactgctgga gaagtcttta taaaagattt
     3001 aacaagaaca tacgatcatt taatggattt acgttttgga aaaaaaagca atggtggatt
     3061 aaccatagaa cgatttacaa tgtttactga ttttgaaatt catggagaag ccgataaccc
     3121 ttatgtggat ataaaagttc acgaaaaagc tttgccttta cttaacaatt tagaaagttg
     3181 ggtacgtttc tctttaacag aatttcgaga tttaaatagc agttatgcta aaacaatgtt
     3241 tagattacta aaacaatacc gaacacaagg atgggctgaa ttttccaagg aagatttttt
     3301 agaactatta gatattccta aaagctatag acaaacaaat ataaatcagt ttgtactcaa
     3361 acccataaaa gaagaactca caccattatt taaaggatta acgattcgta aaaaatatgg
     3421 taaaggccga ggcaaacctg ttattggtta tcgttttact tggaaagctg aaattaatca
     3481 tgctgacgat ttttcaaaag ggaagcaaga agatttacgt acaaaattat ttaatattga
     3541 acacaatgga gaattaacgc aagaagaaaa atggcgcgct aaagatcgaa tactagatct
     3601 tccgttaggt actcatgaag ctaattttaa aaaacaacaa caaactgaaa aagaagaagt
     3661 ggaaaaacaa acactttcaa acgaacttaa acaagattta ttaggaaatt tacaaaactt
     3721 attcgattaa agtataagga gtatttatgg cagataaaac gatcaaagac cttgctgaag
     3781 aattaggagt tagtaagaca gctattaaca aaaaagtttc tgacgaaaat cgaaaacttt
     3841 ggtttgcgaa aatcggaaac aagtttgtga ttaacgaaat cggtcaaaaa gctataaaat
     3901 caatgttttt taccgaaaac gaaaactcaa atcaacaacc agttggcgaa aaatcgaaaa
     3961 ccgaaaacca taaaaacgcc aaccaagttt ccgaattaga actctttttt ttcctagaaa
     4021 aacaattaaa tcaaaaagat atacagctac aagaaaaaga cgaacaatta aaacaaatgc
     4081 aaaaattact cgaccagcaa caacaactca ccttacaagc caatcaacaa atcaaaaaac
     4141 ttcacgaaca actccagcta acctatgcag aagaatcttc agaaaaagaa cccctttctg
     4201 ttactgaaga aactcaaaaa ggacaaaaca aggagaaaaa atggtggcaa ttctggcaat
     4261 aacccaacaa tttttattca taaaaattgt tgggttactt tctttttttg cttttacaag
     4321 catagcgaca tgatctcacg tcgctatttt ttaaagagca taaggaaacg cggtatcact
     4381 ttcttacgaa aggtgctagg taggctgtgc ctaccacgtt tctaagaaaa aaatttgcta
     4441 tcactttttc gccattgtgg ctagcttatg tatagtattt cggtaaaaaa cagctataca
     4501 aatactatat ttgtatgata tacataaatt atacacacat aagaaaggac gttttctatg
     4561 gacattttag ttcgaaatat caatccaaaa attattacaa gagtagataa acgttgtcag
     4621 gaacttacaa ccaaaacagg agaaaaatgg agtcgaaata aatatttaaa agtgctaatc
     4681 gaaagtgatt ttgaccgtca acttatgaat tacaaaaaag agcaattcga tttattactc
     4741 gaaaaattta tcgatattca aacaaaaaat actgaaacat tgaacgacta tgttcataca
     4801 aacaaccaac tcattgaaac acttatcgga ggaatataac atgagaaaaa cttctgttca
     4861 aaaacacgtt accctttatc ccagtcacat tgacgtttta gattccatca tcgaaaaaaa
     4921 tccagatatc aataactact cggaagccat tcgcttttta tgtatgagtt atgaacctga
     4981 taccaataca acaactacca gtaaccaagc caaactaaat gcgatgggaa aagaaatttc
     5041 tatcatgacc gaaattatcg cagaatttgc tgatttgtat ttaaaagata caggcgttct
     5101 tatcggtaca aactcgaaag tatatcaaga agtaaaagaa aaaattgaaa aacagctaaa
     5161 tccctcccaa cgaaatcaac caaagaatcg tcaaaaagaa acgagtcaaa cattcacttc
     5221 taaaaatttc gacccaacca actttctaaa aagataaaaa aataaaccag gaaattttta
     5281 atttcctggt ttattttcta gagagcggtc ttccaataag atttgcaaat cgatcccaat
     5341 ttgcgtaaaa aaaaagctgc tcgtacgagc agctaaacaa gaaaaagtca gcttgtttta
     5401 agggtaaaat aagtgtaaca aattttgcga atgcaacgat cttttttatt atacgcaaac
     5461 aatcaaaaaa aaagactgct cgaaagagca gtctcaagca aaaaataaac gaacaaacgc
     5521 aattactcac gaacacaaac aaatacaata aaatacctaa ataatgaaaa taagtgataa
     5581 aaaacaaggg tatttatagt ttaacaagcg ctcataaaaa taacaagtct ttaaaaaatg
     5641 acagataaaa atgcacaaag aatcggacac agataaccgc gaaaaatcaa gctttagaaa
     5701 gttgttttta gctagaccaa tgaaaaatca caaagtccgc ccctgtatcc gttggtagag
     5761 gggctacttg gtttagtata cattatgtct actagctgtt taaataactg atcttataat
     5821 tttatttaaa cgattatgaa ttcaaaacta ctttttatga cgttaatcat tgttatataa
     5881 taaaatgtta tataataaaa ttactaagat tatttaatat aacaagcaat tttaaaaaaa
     5941 cgaaaaggaa aaacaatcat gaaaaagaaa gtattaaaac attgtgttat tctaggaata
     6001 ttaggaactt gtctagctgg catcggtaca ggaataaaag ttgatgcagc tacttactat
     6061 ggaaatggtc tttattgtaa caaagaaaaa tgttgggtag attggaatca agctaaagga
     6121 gaaattggaa aaattattgt taatggttgg gttaatcatg gtccatgggc acctagaagg
     6181 tagtggtagg gaggttatta taatggattt tactaaagaa gaaaaacttt taaatgcaat
     6241 tagtaaagta tacaatgaag caactataga tgactatcct gacttaaaag aaaagctctt
     6301 tctttattct aaagaaatca gtgagggaaa aagtgttggt gaagttagta tgaaattaag
     6361 tagttttctt ggaagatata ttttaaaaca taaatttgga ttacctaaat ctttaataga
     6421 attacaagaa attgttagta aggaatctca agtatataga ggatgggctt ctattggtat
     6481 ttggagttaa ttctttggaa taaaatgaaa atgggactga agaatcaatt ctgattcatc
     6541 agtcccataa attatagagc cgaaaaatta tcatatttat agatttatta atagtaaatg
     6601 gcataagaat ctttgaacaa cgaaaaaaat gtagctctac ctaattattg atttgcaaca
     6661 atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt tgctagtctt
     6721 ttttgtattg tggagagtgg gggggagggg gattcaaggg ggagcttgcg cccctttgac
     6781 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     6841 cctttagtga gctttagtga gctttactta ttgtaccttt ttaggtacaa tggtattgcg
     6901 aacgatacgt ccgtaggacg tactgaaagg gtgggactta tgagcgaaca aaatcaatat
     6961 ttggctagc
//
LOCUS       DAZUMB010000128         6935 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000128 DAZUMB010000000
VERSION     DAZUMB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.131"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1151)
                     /locus_tag="ACG80L_002359"
                     /pseudo
     CDS             complement(1..1151)
                     /locus_tag="ACG80L_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1394..1849
                     /gene="tnpA"
                     /locus_tag="ACG80L_002360"
     CDS             1394..1849
                     /gene="tnpA"
                     /locus_tag="ACG80L_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS5024565.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2114..2212
                     /locus_tag="ACG80L_002361"
                     /pseudo
     CDS             2114..2212
                     /locus_tag="ACG80L_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2435..2665)
                     /locus_tag="ACG80L_002362"
     CDS             complement(2435..2665)
                     /locus_tag="ACG80L_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS5024566.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2895..3713)
                     /locus_tag="ACG80L_002363"
     CDS             complement(2895..3713)
                     /locus_tag="ACG80L_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5024567.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3874..4563
                     /locus_tag="ACG80L_002364"
     CDS             3874..4563
                     /locus_tag="ACG80L_002364"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5024568.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4577..6079
                     /locus_tag="ACG80L_002365"
     CDS             4577..6079
                     /locus_tag="ACG80L_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5024569.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6092..6568
                     /locus_tag="ACG80L_002366"
     CDS             6092..6568
                     /locus_tag="ACG80L_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5024570.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6665..6775)
                     /locus_tag="ACG80L_002367"
                     /pseudo
     CDS             complement(6665..6775)
                     /locus_tag="ACG80L_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2252 a         1092 c         1265 g         2326 t
ORIGIN      
        1 ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc
       61 ttcctccaaa ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa
      121 aatctcacta ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat
      181 gcctttggca tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg
      241 gcagatactg gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc
      301 tcgataagaa ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt
      361 cttgatcagg cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt
      421 ggtcatccgt aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc
      481 aatctcttga tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt
      541 agggaacttt aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat
      601 gattcgtgcg attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat
      661 tcttttaaaa aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg
      721 cgttcacgtt ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt
      781 ttcttagaca agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca
      841 cggaattgac gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca
      901 acttcacgat aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga
      961 acagaatatt ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag
     1021 gcattgatcc agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa
     1081 tcacttccgc ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta
     1141 tacttagcca taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg
     1201 gtcggtacaa aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt
     1261 tgtgcgtaaa gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt
     1321 tagcgataaa aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga
     1381 aaggagtttt ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa
     1441 gtatcatatt atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa
     1501 agatcttgga aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc
     1561 acatgcaatg ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc
     1621 cagttttatg gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa
     1681 tctcaaatat aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt
     1741 cggattgaat caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat
     1801 tcgatatatt ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag
     1861 tgagtgaaga tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt
     1921 cgaccccttc tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata
     1981 gacttgattt taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa
     2041 tttacggcta ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat
     2101 tagaaaatgc tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt
     2161 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
     2221 agtatattta cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat
     2281 aaataaaatc tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt
     2341 ttaacaagtg atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg
     2401 aagtattttg caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa
     2461 caagatgatt gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa
     2521 acattcgctt agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt
     2581 cctgctcatc gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga
     2641 gaggatgatc cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt
     2701 tctttcaagt tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg
     2761 taggaactac agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat
     2821 caagtatgct ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc
     2881 taagtggggg tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt
     2941 aattcaactg aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata
     3001 taaagttgtg atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt
     3061 tgtaaaacta agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata
     3121 atttttgcgc catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca
     3181 gataaagaaa aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga
     3241 aaatgagggt ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa
     3301 tcaagactag tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg
     3361 atcattttaa tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga
     3421 gcttctgtta aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt
     3481 ttttgactaa agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca
     3541 attttttgac aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca
     3601 ctcatcgtac tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt
     3661 tcagacttcg ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact
     3721 cctctacaat ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata
     3781 ctccattttt atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc
     3841 caaaaataat aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt
     3901 atttcaaaag gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag
     3961 gaagaaagtt taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata
     4021 cgtgcaaatt ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt
     4081 ggaattatta aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa
     4141 gtagatgaat tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat
     4201 cgacatgatg gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat
     4261 cagttattta tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga
     4321 atagattttg taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg
     4381 ccagatatag atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga
     4441 aggattcata ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt
     4501 gtgggtggtg ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt
     4561 tagggggaga aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc
     4621 aattgctata ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga
     4681 gagtgctata aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat
     4741 tatgagttat gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat
     4801 tgctgtagga cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta
     4861 tctagtatat actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac
     4921 actagataca ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa
     4981 tcgttataga aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat
     5041 tccgattgtt tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc
     5101 accaattcaa aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg
     5161 aactttttta tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat
     5221 ttaccctttg ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat
     5281 tgctggagcc caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt
     5341 tacttatggt actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc
     5401 agcatgttat gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct
     5461 ttattttagc ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat
     5521 gtttttattt gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt
     5581 ttattttgct gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga
     5641 ctttttatta ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc
     5701 ttttggtatt gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt
     5761 taaggtagct attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa
     5821 caaaaataca tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga
     5881 aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga
     5941 gattattcaa aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg
     6001 tggcatccaa gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat
     6061 attaggaaaa gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca
     6121 agaaactttt tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc
     6181 tgtcttttca caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat
     6241 ttatgctcca ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat
     6301 aaaaagcaac tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca
     6361 aggagagcct ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt
     6421 ggttgatgtg gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt
     6481 ttttccggag aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg
     6541 gaattcatgt ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct
     6601 aaataggata tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat
     6661 ttttttattt tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat
     6721 aaaagcttcg aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata
     6781 aaatatagag agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca
     6841 acttaggaaa tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc
     6901 actatatacc aaatatagta aaatgctgaa agatg
//
LOCUS       DAZUMB010000129         6600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000129 DAZUMB010000000
VERSION     DAZUMB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.132"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..486
                     /locus_tag="ACG80L_002368"
     CDS             <1..486
                     /locus_tag="ACG80L_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5024571.1"
                     /translation="LIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSD
                     ANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDK
                     ALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQETIKKLRIG
                     G"
     gene            493..1344
                     /locus_tag="ACG80L_002369"
     CDS             493..1344
                     /locus_tag="ACG80L_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS5024572.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            1356..1625
                     /locus_tag="ACG80L_002370"
     CDS             1356..1625
                     /locus_tag="ACG80L_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024573.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            1630..1794
                     /locus_tag="ACG80L_002371"
     CDS             1630..1794
                     /locus_tag="ACG80L_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024574.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            1787..2089
                     /locus_tag="ACG80L_002372"
     CDS             1787..2089
                     /locus_tag="ACG80L_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024575.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            2086..2397
                     /locus_tag="ACG80L_002373"
     CDS             2086..2397
                     /locus_tag="ACG80L_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS5024576.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            2394..2612
                     /locus_tag="ACG80L_002374"
     CDS             2394..2612
                     /locus_tag="ACG80L_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFS5024577.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            2623..3438
                     /locus_tag="ACG80L_002375"
     CDS             2623..3438
                     /locus_tag="ACG80L_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFS5024578.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            3577..3933
                     /locus_tag="ACG80L_002376"
     CDS             3577..3933
                     /locus_tag="ACG80L_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS5024579.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            3893..4138
                     /locus_tag="ACG80L_002377"
     CDS             3893..4138
                     /locus_tag="ACG80L_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024580.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            4135..4533
                     /locus_tag="ACG80L_002378"
     CDS             4135..4533
                     /locus_tag="ACG80L_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5024581.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4530..4868
                     /locus_tag="ACG80L_002379"
     CDS             4530..4868
                     /locus_tag="ACG80L_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024582.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4865..5095
                     /locus_tag="ACG80L_002380"
     CDS             4865..5095
                     /locus_tag="ACG80L_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024583.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            5102..5305
                     /locus_tag="ACG80L_002381"
     CDS             5102..5305
                     /locus_tag="ACG80L_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFS5024584.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            5302..5607
                     /locus_tag="ACG80L_002382"
     CDS             5302..5607
                     /locus_tag="ACG80L_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024585.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGIKN"
     gene            5591..5884
                     /locus_tag="ACG80L_002383"
     CDS             5591..5884
                     /locus_tag="ACG80L_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024586.1"
                     /translation="MELKIDKVTFEEPAFQEMDERIAELVEKKQDELIALLEKKYQFP
                     KYMNKMQAAKYLGVSYNTMMDKYVPAGLKMGIVNGVIRISKEECDRFMKEHQK"
     gene            6144..6560
                     /locus_tag="ACG80L_002384"
     CDS             6144..6560
                     /locus_tag="ACG80L_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5024587.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2470 a          969 c         1497 g         1664 t
ORIGIN      
        1 ctcataggcg atattttcat cgatacaggc atgccaacgg acttctggaa ggaattcttt
       61 tacttccgct ttgaaggtgt cacagggcgc aaaataagcc tcaaagatga atcgaatacg
      121 actgtgagtg atgctaacgt cttagcaaat atcatcttag atttcatctt tgaacatcat
      181 attcctttca aagaaggcta tgagatttta ccagcgaatc aagaatatta cttctacaaa
      241 tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag gagctgatat cgatcacttt
      301 gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc attcagagta cacatttgca
      361 gcactctgca gaatccatca cacggagaaa cacaagatag gtgtgatcaa tttcaaaaat
      421 aaatatcaaa tcaaagggat caagttaaac caggaaacaa ttaagaaact tagaatagga
      481 ggataaaaaa tattggctga caacaaacga tactactatt taaaactaaa agagaacttt
      541 ttcgatagcg acgagatggt tcttttagaa agtatgccag atggatacat ttattctaat
      601 attcttctca aactttattt aagaagtctg aaacatgaag gcaaactaat gtttaatgac
      661 aggattccat tcaattctac aatgcttgcg actattacaa gacactctgt gggagttgta
      721 gaaaaagcag tacaaatatt tcgtgattta caacttattg aggtattaga taacggagca
      781 atttatatgt ctgatataca aagttttatt gggaaatctt caactgaagc tgataggaag
      841 agaaaataca gaaaagaaat tgaggaagca aaacagaatt taataacaag tggacaaatg
      901 tcggacaaat gtccggacaa aactacacca gagttagaga tagagttaga gaaagatata
      961 gagttagaga aagatataga gaatgtaacg ccttcgaaaa aatcgaaggc taagcccgtc
     1021 cgtcataaat acggagagta taaaaatgtt cttttgtcag atgagcagat ggagaaactc
     1081 aaaatagaat tccctaatga ctatcaagaa cgaatagaac ggctatctga gtattgtgaa
     1141 tcatctggta agacttataa aaactatttg gcaactattc gaagttgggc aaggaaagaa
     1201 aaaagtgagc ctaagaatgc aagtggtgca tacaagcgca caggaagacg agagaagctt
     1261 cctgaatggg caatcgacca agaagcctat ctcaagaaaa aagcgctaga acgagctaat
     1321 agacaatcaa aagcaccatt ttaagaggtg gaaaattgaa aatcgattat ctagaactaa
     1381 ttaatgaaat agcaaagtat aaaactggtg aggaaataga aatcctgaga gacgtatatg
     1441 atcaactcga agaagctgga atcgaaggaa ttaataatga tcgttcgagt tggagtaaac
     1501 tcagatacta tttcgcactc tatatcgata caacacaatt aagaaattta gcttatacaa
     1561 aattactatt tgttgattgc gttaaaggat tgcaaaaaca tcttagtgaa cttgagcagg
     1621 tgtaatcaga tggatctaaa aacatttaca gcacagatcg aactaatgca tcaagaagct
     1681 ttaagacaaa gcgcctcgta cgaagacaag tggctcaaca cgttccatgg tggacgtgag
     1741 agcgcacttg atcaagtact caaattattg aaaggggaac gtcgggatgg ataagaaagc
     1801 ggcaatgaaa cgaatcatcg aactgacaca ttctgagaat tggcaagaag acaaagaaat
     1861 agttacagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
     1921 gccgagaaag attgcaattt ggcatggtga tcgaattcta gtaacaggta ctgctgaaca
     1981 gttatctgaa attactggtc tgagcaaaaa catcatctgg gatagagcaa ggagcttatg
     2041 gattgattca aaaggacgac aatttaggta tgtggaggag aaaaaatgaa cgaactaatc
     2101 acaaaagtag agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag
     2161 tttttgaaag tagctgagga attcggggaa atcgcttcag caatggcaag aagtaatgat
     2221 gagctattta aagatagcgt aggagacgta atcgtcacgt tgattatcct ttccatgcaa
     2281 aaagggacaa acgtacaaga gtgtttagaa atggcataca acgaaatcaa agggcgcaca
     2341 ggaaaaatgg tagatggtgt attcgtgaag tcgagtgatt tggaggacag caaatgatag
     2401 gtttaacaat attttggtgt gcgattggtg gtttactagc atttctagga tactacctca
     2461 tctgtgaaag tagaaaagga agaaaatata cagtgacagg tattgtcctg ttaattatcg
     2521 gaattctagt tgcagttgga gcagaactga atctttatgt aaatggtaca caagaagatt
     2581 tggtgcgatt tatgttttgg atgaagaatt aggaggaaaa aaatgaacga aaacaaatta
     2641 atcaaattag gtgtagcagg agcagtaatc gtaggtattg gagttatcgg aggatttaag
     2701 ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa cggcggtatc
     2761 aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt gatccaatat
     2821 ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag cgacggcaaa
     2881 aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc agcaaaaatg
     2941 tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt aaaatctaag
     3001 cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt cctttcagga
     3061 gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt tgaatctaaa
     3121 gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga aacacaaaaa
     3181 tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct agatgcagaa
     3241 actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc agaagcagag
     3301 gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg ttatgaagaa
     3361 gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac tgtagttacg
     3421 gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt tcttaggttt
     3481 agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca aagaagatga
     3541 caacaacgat gaaatgaaat tttaggagga gaaataatgg atctcattac acaatacagt
     3601 gatatcatcc tcaagaaaat catggcaaag attcagaaag ataaaaaagc aaaagaacga
     3661 gcggaattag ttaagttgga aatggctgaa acaggagtag gagtgcgaag tagcaggcat
     3721 tggaaagcag cagcaaacat tgaattttat tacaacgaaa ttcaaaaagg gttcgatcag
     3781 atgcgtgagc tggatcggca aacaaattgg agcaagaaac ttcatcaaga tcgtttcaaa
     3841 tttgtagaga agtataaaga aatattagaa gagtatttga ggaggacagc gaatgataaa
     3901 aaagctcatt caattcagca tggatttata tgatatcgaa tcaggagcaa cagtatctgt
     3961 ggagtctgat catttaatca taagttttgc tgacgaacgt caaattatca tatgggtagt
     4021 tgacgatatg ctatatccag aaattgtaca tgattttgaa gaatcaaaag cggttgagtt
     4081 tgaaatagtg aaaaaagtaa tggaattgat tgaaaaatac gaggaggaca gcaaatgata
     4141 ccgaagttta gagcgtggga taagcgagaa aacacaatga gagatgtagc tgtcttacat
     4201 tttactaaaa acggcaaaac aaactttatt gaatattgga taaatcctac cgaattgaaa
     4261 tcatatcacg tgcgaaacat cgacctcatg caatccacag gaatgaaaga taagaatggt
     4321 gtggagatat ttgaagggga tgtagtaatt acatcaaaag gagcgattgg atatgttgcg
     4381 tatcttcaac aagaagccgg ctttgtagtt gtactaaaga aaagtgatta tagattaggc
     4441 cataggaata ctggcgaaag ttatgatgta gcaactgctc atgaagtcat cggaaatata
     4501 tacgagaata gcgagttatt ggaggtagag tgattatctt agctactgac tacatcgatt
     4561 ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa ttgtggaatt aaaagcaaga
     4621 atagaatcat tttgttacgg atatcggctg atttagcaaa atatctttat ctgagcaaga
     4681 cagcagtata ttacactgcg gctacacgtt atcagtacaa gggaaaaaca atcagtcagg
     4741 attattatga tcgtttaatc agtcctgaca tgcatggaaa atcaaaatca gcgatcaagt
     4801 tatttggcgc aatagagata tatgccgatg attttccaaa tttatggttg aaggaggaag
     4861 cggaatgaaa tacgaaatac cactaagtga agcgggcatt caagcaatta tcaatggtcg
     4921 ggaggttaat aaaaaacttc ctgatggtac tgaattagtc atcagacaaa gttatttgaa
     4981 agatatggca gctccagtat taattgatcg ttttaacgtg actgattctg tggtagagaa
     5041 ccacttgaaa gaatttcgat caagtataaa cgacactttc agattaggga gttgattgac
     5101 aatgaacacc agacaccgca gagtagcaaa actaagaaaa caggaactga atgtactaaa
     5161 gacaaagttt gaaaaagaat atggaatttc agcagaagaa acatataaag tggcaagtca
     5221 gtttgttgct gatgcgagtg agactattcg taagtttggg atttcgatat taaatgatga
     5281 tcgtaaatgg gaggcagaaa gatgaaacta aaagacggat tttacactga tgcttatgga
     5341 attggtggtt tgatgatgga tctaccaact aagaatccta taaagcaaga ggaatcagaa
     5401 attaaagtcg gtgacatggt tcgctgcgaa gcagaagggt tcatctatcc atttcgtgga
     5461 tatgtagagc atctctataa tcactcagcg atcattcgta ttgaaaacac aatggaatgc
     5521 gataagtggt tagcgaaaag caaagagaat ttagcagtgg tgagattggt ggatatggag
     5581 gttataaaca atggaattaa aaattgacaa ggtaacgttt gaagaaccag catttcaaga
     5641 aatggatgaa cgaattgcag aattggtcga gaaaaaacaa gatgaattga ttgctttgct
     5701 agaaaagaaa taccaatttc cgaaatatat gaacaaaatg caagcagcaa aatatcttgg
     5761 tgtaagttac aacacgatga tggataagta cgtaccagct ggtttgaaaa tgggtatcgt
     5821 taacggcgtt attcgtatca gcaaagaaga atgtgatcgt tttatgaaag aacatcaaaa
     5881 ataaaataaa aaaacgttta gccccttagc taaacgcaca attataattt acctgacagt
     5941 taattataac aaaattgcgg aggttgagga atggcggaat gaaaaagaca ataaaaacag
     6001 aagaagcata tattgaagta tttgacacac cagcatgcta agtcgagaag tgtaactcgg
     6061 caaataaaaa aagccggatc gctccgactg atgtaataaa cccaacaagt ttattatatc
     6121 acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa
     6181 gatgcaatgc gagagatgtg ctgaagaatt ttcgacgctt agagcggatg gcaggtcgct
     6241 ctttgataga tattaagtcg ccgattatta cggatatgcc gaaggcaccg aagcacggca
     6301 ataaggcaga ggacgcgatt attcagatga tggatataga agcagagaga gatgcgattc
     6361 tagcggcttt gatggctctt agtctgatta gccgtcagat actctattat agcttttgtg
     6421 acgtaaacaa gcactctaat tatgaaatag ggcaattgat acgaggatac ggagagaaga
     6481 atgtagagaa gctgaaatct atcgcgctga tcgaatttgc tgaagcatac aaaaaaggcg
     6541 tattagttca gtatcgttga ttttgtaggg tttttgtagg gatagtgtag ggtttttgag
//
LOCUS       DAZUMB010000130         6550 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000130 DAZUMB010000000
VERSION     DAZUMB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.133"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..1015
                     /locus_tag="ACG80L_002385"
     CDS             8..1015
                     /locus_tag="ACG80L_002385"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFS5024588.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1027..2031
                     /gene="neuB"
                     /locus_tag="ACG80L_002386"
     CDS             1027..2031
                     /gene="neuB"
                     /locus_tag="ACG80L_002386"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFS5024589.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2028..3176
                     /gene="neuC"
                     /locus_tag="ACG80L_002387"
     CDS             2028..3176
                     /gene="neuC"
                     /locus_tag="ACG80L_002387"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS5024590.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3149..3826
                     /locus_tag="ACG80L_002388"
     CDS             3149..3826
                     /locus_tag="ACG80L_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS5024591.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3777..4958
                     /locus_tag="ACG80L_002389"
     CDS             3777..4958
                     /locus_tag="ACG80L_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS5024592.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            4971..5948
                     /locus_tag="ACG80L_002390"
     CDS             4971..5948
                     /locus_tag="ACG80L_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5024593.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5948..>6550
                     /locus_tag="ACG80L_002391"
     CDS             5948..>6550
                     /locus_tag="ACG80L_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5024594.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEIN"
BASE COUNT         2440 a          823 c         1250 g         2037 t
ORIGIN      
        1 gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt ccattggaag
       61 tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt atagtagagg
      121 tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt tgagattttt
      181 aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg tggatattat
      241 ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta tggaagctgt
      301 aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa ataatgtaaa
      361 acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca tgggtgcgac
      421 aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa aaacagaaac
      481 aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg ttattcctct
      541 ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg atatgacacg
      601 atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta tgaattcaaa
      661 atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt tagctcaatc
      721 attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa taaaagttgt
      781 aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg aaattgaacg
      841 ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta ctgacgaact
      901 gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa cagaattaat
      961 gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt ggtaaggagt
     1021 tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca taatggaagt
     1081 ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa cgcggttaaa
     1141 tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc aaaatatcag
     1201 aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt agaattatct
     1261 caatctgatt ttattagaat aaaagattat tgtatacaaa ataaaattga ttttttgtct
     1321 actccttttg atgaagaaag tgcagacttt ctaaaacaaa ttggtgtaca aggatttaaa
     1381 attggctcag gagatttaac aaatttacct tttcttagaa aaatagatat gtatgggttg
     1441 ccagttttat tatctacagg tatggctgat attcatgagg tcagtgaggc tgtagattgt
     1501 ttcatagata gtccagttac tattttacat tgtacttcaa attatccggc tagttctgaa
     1561 gatattaatt tattggcgat taacacatta aaagaaatgt tcgggatacc cgttgggtac
     1621 tccgatcatt cattgggata tgatgttggt atttgtgctg ttgcgatggg tgctaaggta
     1681 attgagaaac attttacctt ggataaggaa ctacctggtc cagatcataa agcttcttta
     1741 gatcctcagg aatttgcaga ttttgtaaag catattagaa acactgaaat atttttaggt
     1801 gatggtgtta agcgagcaat gccttctgaa cttagtacaa aagaagtatc aagaaaaagc
     1861 ctagtggtca ctgagaattt aaatgtggga gatattctta cagagaaatc tcttacaata
     1921 aaacgtcctg gaaatggaat agctcctaag tttttatatc aatatattgg caaaaaagtt
     1981 aaaagaccaa taagtgcaga tagtgttctg cttgaggaag atgttgaatg aaaaaaatac
     2041 ttatagtaag tggaacacga gctgattatg gaatatataa acctgttgct gagaatttac
     2101 tacaagataa ttgggatgta tcatttgttg tttctggaat gcatttatcc cataaatatg
     2161 gatttactaa agaattgatc ttagctgacg gatttagaat tagtggagaa gttcaaagtt
     2221 tgtttttaga gaacaataag ggaaacatgg ctcggtctgt ggcattagag ataaatggtt
     2281 tcacaaatat atttgaacaa actagccctg attttgtatt attactaggg gatagaggag
     2341 aaatgttggc agctgcgata gcttgtttgt atcttggaat caaaacagca catattcacg
     2401 gaggcgaagt gtctggtact gtagatgaat caataagaca tgccataacg aaaatagcat
     2461 ctgttcattt tgctgctacg gaaaaaagca aagagcgttt aataaaaatg ggggaagatt
     2521 cttggagagt ttttcccgtc ggagctccta gaatagatac tattttaaat aatagattac
     2581 ctagttttag agatataaaa gaaaaatatc atctaaaatt tgaaaagaaa aaatacgttt
     2641 tgcaagtatt tcatccagtt gtaaccgaac tagaaaatat agaaaaacaa attttagctt
     2701 tgatcaattc aatggaacaa caatctcaac caattatttg cattttacct aatagtgatg
     2761 ctggtagcga aattatcaga aaaatgtacc agaaatattt aaaaacggat attatttata
     2821 ttacgaattt agagccggag gaatacctga ctatattaaa agagtgcaaa tttatgatcg
     2881 gaaattcttc ttctgggata attgaagctg catcattcaa aataccagta ataaatttag
     2941 gaaccagaca aaatggacga gaacaatcta aaaatattat aaatgcaaat ctaaatgaaa
     3001 atgaaattga aactgctttg aataaaatac aagatcctca gtttataaaa agcttgaaaa
     3061 atgttaagaa catctacggt gacggcaaca gttctagaag aataacaaaa acattgaatc
     3121 aaatattgaa tgatggggaa gatattaagt ggattcaaaa acaaatagct tattaaaata
     3181 tactagtgat ccatacatta ttattggggc tggcggacac gcaaaagtca ttattgaaac
     3241 cttaagatta gaaaataagt taatatatgg attttttgat gataagcaaa ataaaatgca
     3301 agatctgaaa tatcttggga aaataaacga attgaatagc tgggcggata gcaaaaaaaa
     3361 aatctataaa tttattgtag ctattggtga ctgtgtagta agaaaaaaga ttataaaaaa
     3421 attgaatatg ccaagtgagc aatatggaat agctgttcat ccaagtgcta tagtggcaaa
     3481 agaaatttca attggttatg gaactgttat ttttgcgaat gctgtaatta acccagatgc
     3541 tgtaattgga gagcatgcta taataaacac aggtagcatt gttgagcatg attgtagaat
     3601 taataactat gttcatttat ctccaggtgt atgcttagcg ggtggagtac atgtaggtgt
     3661 aggaactcaa ataggaattg gttctcaatg tatccagatg aagaaaattg gatcttggtg
     3721 tatgataggc gcagggagca ctattgttaa ggatattccc tcacattcct tagcttatgg
     3781 caatccagct aaaattaaga aagaaggaat taattttgaa ttctagaatt ttattatcaa
     3841 gtcctcatat gggaggaaca gaagaaaaat atgttgctaa ggcttttaaa gataattgga
     3901 tagcgccatt aggtgaaaat gtggataaat ttgaagaaag tattacaaaa tatactggtt
     3961 ctaagggcgg agcttctgcg ttaagttcag gtacagctgc catacatctt gccttaagac
     4021 tgcttaatgt tggaaaaaac gatattgtct ttgctccttc atttactttt atagctacag
     4081 taaatcctat tttatatcaa ggggctgtac cagtgttcat tgactcagaa cctaatacat
     4141 ggaatatgag ccctgttgca ctagagagcg cattagaaga agctgaaaga aacaaccaat
     4201 tacctaaggc aatcatcgta gtgaatttat atggtcaaat ggctgattat gaacgaattt
     4261 tagctattgc aaaccaattt aatgttccag tgatagaaga tgcagcggaa tcgttaggtt
     4321 ctacttataa gggaatttcg tcaggcttat ttgggacaat tgggatctat tcatttaacg
     4381 gtaataagat catcacaacc tcaggtggag gagctctagt gtctagtgat aaaaaatatg
     4441 ttgagcattc aagattttta gcaacacaag caagagatca agcaccgtat tatcaacata
     4501 gcgaaatagg ttataattac cgtctaagca atatttctgc cggtatcgga cgtggacaaa
     4561 tggaagtatt gaatgataga gtagctaaac gaagagaaat tttcaatcga tataaggaag
     4621 ctttttctgg aattgataga attgaaatga tgccagaagt ggatggcatg gtgccaaatc
     4681 gttggttgtc tactttaacg atcaatgatt ataattctat tgatcaagta gttaacgtaa
     4741 taaataaact aaatgaaaaa aacattgaaa caagagcttt gtggaaacca ctacatttgc
     4801 aaccagtatt tcaaggggta aaaatgtatt accatgataa taatcttagt gtgtcagaaa
     4861 agttatttaa tagcggctta tgcttacctt ctggaagtaa tatgacagat agtgaacaag
     4921 aatttgttat tgatgagttt aaaaaaatat tcaagtaggt gtgatatttt atgaacatag
     4981 gaattattgg tttaggttcc atggggaaaa gaagacttag actcattcaa gaacacttta
     5041 atgatatttt actatgtggt gttgatacta atgaggcgag acgaaaggaa gttgaaaagg
     5101 aatttggaat accaacttat aatagtctaa cagaggcaaa aaatttgttt gaaataaatt
     5161 cagtttttgt ttgtacatcc cctataaccc atgaatttat tataaaagag gctttaaatt
     5221 tagattgtaa tatctttacg gaaatcaatt tattaaatga atattatgat gaaataatcg
     5281 aagtagctaa aaagaaagat agattacttt atctatcttc tacatttttg aagagaaaag
     5341 aaattcaata tattaagtct aaagtgacgc ataataaaaa aatttcttat caataccatg
     5401 taggacagta tttacccgac tggcatcctt gggaagatta tcgcaatttt tttgtttcaa
     5461 acaaaaaaac taatgggtgt agagaaatat tcgctattga attaccttgg attatagata
     5521 catttgggaa agttaaatcg tttactgtaa tgacccagaa attatccaat cttgatattg
     5581 attattcgga cagtttcact cttattattg agcatgaaac tggtatagtt ggaactttaa
     5641 gtgtagatgt tgtttcaaga gtagctaaaa gaaatttaac tataattggt gaaaagattt
     5701 taatagaatg ggaaggtaac ccggattctc tattttcatg gaatgaagaa acttctagta
     5761 tgacagatat tcaattgtac gatgaggtta tgcatgataa taattattca catactatta
     5821 ttgaagatgc atatttagag gagattaaag aatttttcac gcttttggaa aagaaaatgg
     5881 aaccccaata tacttttgaa aaagataagt atattattaa cttaattaac gacattgagg
     5941 taaattaatg atgaaagtaa ttttttttgg gttagggtca attggaaaga gacatctaaa
     6001 aaatctaata agctatggaa aaaagaattc tattaatttt gatataacag cttatagtag
     6061 cagtggaaaa aaagagtttg aagaaataac gtatattcaa aatagagacg atttggatac
     6121 acattatgat atagctttta ttacaaatcc gacttttaag catctggaaa cactaaatgt
     6181 aattgttgaa aaagttgatt acatattttt agaaaaacct gctttttctg agagtattga
     6241 tcttactcaa tataattatg attacaaaaa tatttatgtt gctgctccac tgaggtataa
     6301 aaatatcatg gaagttgttc gtaatagtat tcaaagtaaa aaagtatatt ctgtaagagc
     6361 tatttgttct acttatcttc ctgattggag aaaaggtact gattatagaa ataattattc
     6421 tgcctttaaa tctatggggg gaggagtaga attagactgt atacatgaat tggattatat
     6481 tataaaccta tttggtttcc ctaaatcagt aaaaaaacag tttggtaaat attcgaatct
     6541 tgaaattaac
//
LOCUS       DAZUMB010000131         6366 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000131 DAZUMB010000000
VERSION     DAZUMB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.134"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1831)
                     /locus_tag="ACG80L_002392"
     CDS             complement(<1..1831)
                     /locus_tag="ACG80L_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5024595.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETF"
     gene            complement(2043..2939)
                     /locus_tag="ACG80L_002393"
     CDS             complement(2043..2939)
                     /locus_tag="ACG80L_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5024596.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2932..3612)
                     /locus_tag="ACG80L_002394"
     CDS             complement(2932..3612)
                     /locus_tag="ACG80L_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5024597.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(3613..4395)
                     /locus_tag="ACG80L_002395"
     CDS             complement(3613..4395)
                     /locus_tag="ACG80L_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5024598.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(4388..5305)
                     /locus_tag="ACG80L_002396"
     CDS             complement(4388..5305)
                     /locus_tag="ACG80L_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5024599.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(5469..5723)
                     /locus_tag="ACG80L_002397"
     CDS             complement(5469..5723)
                     /locus_tag="ACG80L_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024600.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1886 a         1296 c         1030 g         2154 t
ORIGIN      
        1 tgaatgtttc ataaatttta ttcaattcag gttttacttt ttcattaagt gcaaaaaaga
       61 aaacagaatg aacaagtgtc ccatcccaat caattccttc tggattaacg tagacacaga
      121 ttactggctt ttttataaaa tctacttcga tatgcggcat agcaaaatca ttcatcgctg
      181 ttgaagatag tttttctctt ttgattgctc cctctagtat cccttctttc gcgtaatttt
      241 cttttaccaa ttttttggta attatattta aaacgttcac ttgattatcc tttatttggt
      301 gggtaaaaaa gcaaaatcgt ttatctaata aatttagaaa agcatcttct gtttgttcat
      361 ttaactgtag atactgtttt acttttctga catctgcccc taacatcaaa ggactaacaa
      421 caataacagg aaaatttgta ttttcaatcg gaatcgtgct tataacaagt tcatcagtga
      481 cttttctatc ttgtagttca ttgatgctta gtacatcttc tatctcaacc atatctgcaa
      541 aaccgttatt tattctttgc tctaataatt ttgatgttcc atatccgcta ctacaaacta
      601 atatcacttt cgttttgtca ggtttgtaac gatccaaaag tgattgaata tggagtgtta
      661 cgtaagcgat ttcatcttca ttaaaatgta tacagaatct ctcttcgatt tcctctaata
      721 aatctacact tatcataaat gaccttttaa aagaatattt gattttgtcc gtataagggt
      781 tatagatatt tactcccaat ttgagtcttt taactgctgc attgagatgt aatactagat
      841 tattgatcaa ttcttcatca ggataaaatt catttaattt aattcttact aactcttgta
      901 gctcaacacc catatctaaa taggaaagct tcgtttgatc taagtcgtga ttcatagcat
      961 ttttttcaat cgaagtaatg tgaatgtcaa cataagcttg ttcatctatt ggcacaataa
     1021 ttgaaaatgc tttttcaatc attttgatta aaataagtgt attctttgag atttcttctt
     1081 cagaattatt actacgtgat aagaaattgc ttgtttcaat cctttctatc gcaatagcaa
     1141 ggtggatagc taaagtttga aattcataat ctgtaaattt caattgtgtc ttattcatgt
     1201 atcgattaag caattcggct acttttttca atatgtcctt atttaagaaa ttattcgctt
     1261 caccaatgaa attgatattc aagtctaaga gatctgtttt actgttagat gctgtaaagc
     1321 caccccaata atagttcaca acttctgaca tcaaccttct tttacttttt tcatcagctt
     1381 tgatgcgcac gccatttcga tctgaaacta aggacatccc atgcttgttt agtctttttc
     1441 taacatcttt cattgtgctt tctaccgtcg acctgctcac gtataattcg tcagcaagat
     1501 tttgaatctt tagagaatca ttagtattca ataatttagc aaacacataa gttagacggt
     1561 cctccggaga gtctgcgata ttgattttat gactggataa taaatttcga agtgtttctt
     1621 tttttccaat aagcgaaacg ccttttttag gttgaaccaa catttgtaca tctggcattt
     1681 tttggattat ttctttcaaa attaaaatat ctttgctaat tgttttcgta gatacatata
     1741 attgttctga taattctttg taagttgtgt gctcattttc aatcaaaaag tatagtattt
     1801 tcatttgccg ttcgctaaat tcgatgccca ttatcttcac ctcctgaata cagcttaagt
     1861 gtacaattat tatctctatt ttcatacaga agaaagttct tgattaatgt agaagtttct
     1921 aatattcttg attatttcaa atcaataaaa aagcctgcaa tatttatatc acaggctttt
     1981 ttctctatta aatatatata agataataca tttttctact agttctcact aatcttgtat
     2041 aattaaaccc tccgcaactt gacggagatg gagaagatgc ccccgtctaa tctagcggtc
     2101 atctctcctc ccattccttc caccaatttt tttgacaggt aaagcccgat tccgctagat
     2161 tccgtatttg tgcggctggt attttcggta taaaaacgct ggaagacttt ttcaacagag
     2221 gtttgcgttc ctggtttgat aggattggcc actgttaatc gaatgaatgg atcctctttt
     2281 gaaaatgtca cctttgcttg tccatctgca taacgcaata aattctgtat gatattttgc
     2341 agcactcgca gcaacatgag cggatctgct aatacagttg tatttatcga gtgttcagcg
     2401 aacgctacct cgatattttt ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc
     2461 acctctttag aaaaagaaag cgtatggaga tccatttctt tttgcttcgt ctctattcga
     2521 gcaagatcat aaaactgatc cgtcatactg agtaatcttg caacagattg tttgatgatc
     2581 gttaaatact tctcccgttt ctccgtatcc tctacctgtt ccagtaattg tacataaccg
     2641 ttaatagcag tcaaaggagt tctgagatca tgagaaaggc cgacgatcga tagtctgatg
     2701 ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat tttcatccaa ccatgtattc
     2761 agtgcatcta ctaggtccat tagattcttt tctcgaaatt cgactgaaag acgaccacca
     2821 tattcttgtc gttcagaaag agtggcgatt ttcttagtta atctcttgat ttccctttgc
     2881 aggaagaaaa taaaacttcc cataaagata acaacgagaa gcaacagcca tttaatcaaa
     2941 tctaaacccc actccccaca ccgtatgaat atacacagca tgtgcctttg ctagcttccc
     3001 tcgaagattg gaaatatgca tattcactgt tttctcacta tcaaaatatg gttcttgcca
     3061 aactgtttca taaagatttg ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt
     3121 cagcagatcg aattctcttc ctgtcaaatg gacagccaca ccattcacat ttacatccct
     3181 cgactctttg tccagtaaga tttccttgta atagatgcgt tgcgacagat cttttacttg
     3241 gacagaagtc tgatgacgaa gttggatgcc gattcgtaac aaaagttctt cgatatcaaa
     3301 tggtttcgtc acatagtcat ccgctcctgc tgccaacaag gaaactttat ctctttgatc
     3361 gctttttgct gaaagaacga tgatcggaac attcgatgtc tgtcggattt ctgctaacaa
     3421 tgcttcacct gaaagccctg gaagcatgag atctagaagg atcaaatcga caggatatgt
     3481 gcttaacaat aattttcctt ccgtacctga ataagcatca agaagtttgt atctctgttc
     3541 aagaacctcc tccagtaagg aatgaacagc ttcatcgtct tcgataatca aaatagtttc
     3601 cagcaggacc acctacttaa tgtcagattt ttggaaaata aacattccta gagctgatga
     3661 taccacgatt gttactagac tgaccagaat cattttcggt gcttgcgaat aagttgcttg
     3721 ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac cactccgtta gaaattccaa
     3781 gcctttgatt tttgcgaaaa caccgggaat gaaggccgtt cccatgatat aaagaaaaga
     3841 aagaagcaaa ctgacactgg tacttctagt gatgaatgtc aaagccactg gaacagataa
     3901 tgtccctaat gtcagcagaa gcaatacagg aaatactttt agaacataat ttccatagtt
     3961 tttgattccc tcaacttcac caaatccgaa agcaagtgtg taagcaagca tggctagtcc
     4021 cgtgatcaaa accacgccaa gtgttgtgat caaaagcgta acgagaagtt tcgacacata
     4081 ataagtcatt cgattccgtc ctaaagataa cgtattgcga atcgtaccgt tttgaaattc
     4141 ttccccaata taaaagcacg caaatgctgc gaaagccaga agaatccatg cagccatttt
     4201 ttctaccatt aaaatccctg ttgcaccgtt gatgactgta tccgttgctt ggtttaattc
     4261 attgccgtat tttgtatgca tataccattc gttccaagaa taaaaaagcg gaagtacgac
     4321 catcagtcct agtgagaccc acattttacg gctcttccat acttttattt tttcagcagt
     4381 taatagatta aacatgtgga ttgcctccag ttaattgtaa gaaataatct tccagttttt
     4441 gcccaactag gtgaatacca tccacaggaa cttttgcttc taccaatgct tgattgatgg
     4501 ctgctacttg gtcaattcct tcaaaaagct ggataacacg gctagattgt acaaaataat
     4561 ccctataacc aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt
     4621 ttataaattg acgagattct tgtgccaatt cttctttcga taattctttg atcaagtgtc
     4681 cttcatgcaa aataccatag tgagtggcga tctgtgacaa ttcatctagc aagtggctag
     4741 aaagcaagac ggtgatgccc cgttctttta ccaaacgttg gatgatttct cgcatttctg
     4801 ccatccctgc tggatccatc ccattcgtcg gttcatccaa aatcaaaaat tctggattcg
     4861 tgatcagtgc atttgcaata gctaaacgct ggcgcatacc taaagagaaa ttctttgctt
     4921 tctttttccc tgtattttcc aatcgcatca ttttcagcag gtcttcgatt tctctatccg
     4981 acacaccgcc gttcgctgct tggatacgta ggttatctct tgcagttaat tctggataaa
     5041 gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc
     5101 caaataattc gatcgaacct tcatcaatcg agatcaaacc cataatagtt ctcataaagg
     5161 tagacttacc cgctccattt tccccaatca atccatagat cattccacgt ttgatttcga
     5221 ttgatacttt atctaatgct ttggcagcac cgtatttttt ggttatacca gttgctttca
     5281 agaccgtttc atttccaaaa tccatactta tccttctttc ttattgatga gtgaagtata
     5341 gttgacgggt ataaagaaag cagccagaaa tgtataaaga aagtgtaaat tttccaattg
     5401 aatgtcaaaa aaaacaatcg gacaatacgt gtctttccta ttatcaggca gtttgttata
     5461 cgcttgtatc agtttcctga ttctttcttt tcgaccaaag tcgtttctct gtattcctct
     5521 tcactgaaaa tttttgcttc tttcagatca agcttgagta attttaccag accggttata
     5581 aggataacta tagcaagact ccctcccaga aaaattagac tgtctcgcag acgtggttgc
     5641 gttgtcttcc aatcaaagag cgcaccaaac agtgctaatc caagtggact ggccaccatg
     5701 gtaatcgttg ttatcgttga cataagtcgt ccttgtatat gcgttggaac ttctcgctga
     5761 agccagacca tataaaaagc aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa
     5821 taatacatgc cgtttgggat ttacaattag ctaccaaata agaggttatg acaaaagact
     5881 tttcacaatc ccttcttccg aataaacggt gttctcctgc caactcctcg gtcacaagtc
     5941 acaatattta tcaaacatga gaagcaattt ttgtaaattc cttcttattt cttgaagctg
     6001 accacttctg tcacaacctc agaagcggat aaataatatt gtttgatgat actttatctt
     6061 tttcccgttt gtttatagat cgaatgatat tatagaccaa gaatcctacc tattaacata
     6121 gctaaaaaca caaaaaaggc actttccaaa ttgtggaaag tgccagaaaa gttaggatta
     6181 aggtaactgc gctcagcatt gcttcgccca gtacgattct cgtaactcgt gaactcgaac
     6241 gataatcgca attaacgttt tgagaactgt gaagctttac gggctttctt aagacctggt
     6301 tttttcttcg tatcattgaa taagtttgaa aaaccttgat ttataaaggt tttcccgtaa
     6361 atatat
//
LOCUS       DAZUMB010000132         6362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000132 DAZUMB010000000
VERSION     DAZUMB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.135"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACG80L_002398"
     CDS             complement(207..1169)
                     /locus_tag="ACG80L_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5024601.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACG80L_002399"
     CDS             complement(1171..2610)
                     /locus_tag="ACG80L_002399"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS5024602.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACG80L_002400"
     CDS             2795..4741
                     /locus_tag="ACG80L_002400"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS5024603.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /gene="scrK"
                     /locus_tag="ACG80L_002401"
     CDS             5005..5877
                     /gene="scrK"
                     /locus_tag="ACG80L_002401"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5024604.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6173..>6362
                     /locus_tag="ACG80L_002402"
     CDS             6173..>6362
                     /locus_tag="ACG80L_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5024605.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKR"
BASE COUNT         2039 a         1103 c         1121 g         2099 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aatcgtaata cccgctaaag catacaagga gagtgaacat aaatgactta
     6181 tacccatctt acaccaaacg agcttgtaat gatagaagca tattttcatc aagaaactcc
     6241 ggttgctatc gttgcgaagc agcttaaacg tggacgccaa acaatttaca atgtctataa
     6301 ctttctcaaa tgtggtggaa cagcacttga atgctttgaa caatacaaag aaaataagcg
     6361 ac
//
LOCUS       DAZUMB010000133         6289 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000133 DAZUMB010000000
VERSION     DAZUMB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.136"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..341)
                     /locus_tag="ACG80L_002403"
     CDS             complement(<1..341)
                     /locus_tag="ACG80L_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5024606.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGV"
     gene            complement(338..859)
                     /locus_tag="ACG80L_002404"
     CDS             complement(338..859)
                     /locus_tag="ACG80L_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5024607.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(861..1895)
                     /gene="sppA"
                     /locus_tag="ACG80L_002405"
     CDS             complement(861..1895)
                     /gene="sppA"
                     /locus_tag="ACG80L_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5024608.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1928..3343)
                     /locus_tag="ACG80L_002406"
     CDS             complement(1928..3343)
                     /locus_tag="ACG80L_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS5024609.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3312..5465)
                     /locus_tag="ACG80L_002407"
     CDS             complement(3312..5465)
                     /locus_tag="ACG80L_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS5024610.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5723..5959
                     /locus_tag="ACG80L_002408"
     CDS             5723..5959
                     /locus_tag="ACG80L_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS5024611.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            5978..6193
                     /locus_tag="ACG80L_002409"
     CDS             5978..6193
                     /locus_tag="ACG80L_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS5024612.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT         1997 a         1150 c          936 g         2206 t
ORIGIN      
        1 actccaggaa ttacccacaa taatgttaaa agaaaaaatg gaataataga taaaatgagt
       61 aaactaatac catagaccac tgttttttga ttttttctta ctaaaaaacc tattatgaaa
      121 atcattatcg aaaaaactag ggtaattcct ccaaataaaa caacattccc gcttagattg
      181 actaacatat ctatatcctt actggttagt tctgtagtat ttaaagtagt tgaatcaata
      241 gaagatagaa acataccacc catcaatgct aaaagactac ataaaatccc tgttattcca
      301 ctaattaaaa tcaaaataaa ctctgttttt ctcttattca tttaattctc tccagattaa
      361 gtgttttaga attagaaatt ttttgaccat agttaatagc ttctaatgaa cgaataacaa
      421 ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg aaagcagcca
      481 ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata cactcacgaa
      541 taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt atcccaaaaa
      601 tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa taggttaaat
      661 aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat tccagtccca
      721 tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa attaaatcaa
      781 taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc aaagtttcaa
      841 tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt tggagtatca
      901 gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc ctttagccct
      961 tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt gtcattttta
     1021 tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc taatgcatct
     1081 tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc atcataaatt
     1141 cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt tgctacaaca
     1201 ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt ctcttcctct
     1261 gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata cgtcgcatca
     1321 gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat gacaccaatt
     1381 gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga aatgtagtac
     1441 cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag ctttttaatt
     1501 tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg tgaatttact
     1561 tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg tgttaaaaaa
     1621 ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc tgcaataact
     1681 ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa cgtttcttct
     1741 gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt atttgatgct
     1801 tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa taatccgcac
     1861 gctactccca cagcaatcca tcttctttta ttcatataaa ctcctcctat attcttagta
     1921 aaaataatta ttttttattt agaaacacat cgacaatttg ttgccaatat gttttttctc
     1981 ctacaattaa agacaactct ccagttaatc cataacgaaa cggaaacgat tgagttgttt
     2041 ttaacgttcc tttcactgca taaaaaacac ctttctcagt ggcagtagaa ttttctgaaa
     2101 tttcatctaa agttccgtcg atcgtaatcg gagccccacc tttttgatcc aatttaaaat
     2161 gaaccttcat cccttcttta atataggtac tttcattcgc aggtaaaagt gctgtaaatt
     2221 tcatttttga tttttcgtgg atcggataaa tttctgcaag cactgttcct ttggctagtt
     2281 ctgcttgtcc ctttgtttct tcatttaaat gaaccgttcc agatacagga gcttttagta
     2341 ttccttttga aagttgatcg tccaaacttt tgataactga ctggttcttc tcttgttttt
     2401 catttagctc tgtaactgtt tgttttacag ttgttattgc ttgttctagc tcttgctttc
     2461 ctttttccgt ttgactaatt ctgtcattct cagaagtagt tggtttaact aatttagctt
     2521 tttctgactg atactgttct acttcttttt ttagttgctc aatttgttca tttatgcttg
     2581 ctgaaattgt cagtttcact tgattttttt gatcatcaga aacattattt agttgttctt
     2641 gccaattctc atagtttgcc atcatttctt ttgaaaaatc ttttaatgat tgattattac
     2701 tccaagctac ctgtacttgt tgccattctt gtaatttact atctgaactc tcaatttgtt
     2761 tttctagttg tctttttgtc ttttcgtata cttctaattg cttttgatca tttaattcac
     2821 tttgcttcaa agcgtagcgg agtgattctt tttctgacaa catgctcttt aattgattac
     2881 tgtagccaaa actgtcatcc gttgaaaaca aattttttcc tttacttata ctttcaataa
     2941 atagctgagc catctttatt tgttgagtaa tacgttggtt ttcttgttct atctgagcct
     3001 tttcattctc tattaaagaa caatccaatc taaccaacac atcgtcctta tgaacaaatt
     3061 gattttcttt taggaagttt tccattattt tagcttctaa tggcacttgt aacttctcta
     3121 ttttttcggg gttcaattga gccgttgtcc gaataacaac ctctttttta gcaaaggtta
     3181 aaaacaatcc aataatacat aaaaaaagaa ttacagggta taacacccaa cggtagaagc
     3241 tatgatgttg tttagtatag atactagaat gttcgaacca attattattc tgcataaatt
     3301 gtttccctct cctagatttc ccataatttt tgatagatac catcggcaaa acgaagttca
     3361 tcgtgcgttc ctttttctac caaatgccct tcatggagaa ctaaaatttg atcacatgct
     3421 tttgctattg gtaaatgatg cgcaataaaa ataatcgttt tttcttttaa attgaggaga
     3481 taattcataa ttgcatgttc taacaaggta tctaatccac tagttgcttc atctaaaatt
     3541 aatatgtccg gattttttaa taaagcccgt gctaaggcta atcgttgtcg ttgccctcct
     3601 gaaagattat ttccaccttc ttccaacaat gtctcgtaac gtaatggctg ttgattaata
     3661 aattcattta gttggactat ctcacaaact tctattattt gttcgaaact tggtgggcta
     3721 tctaaaccga atataagatt ctcaatgatt gttccactaa agaaaaatga ctcttgaggc
     3781 aaatatgtca ctcgatcgcg taatatctga aaaggaatgt ctaagtgatt aatgtttcca
     3841 taatggatcg atccttctga tgcttcataa aaacgaacaa ataactttgc aagagttgat
     3901 tttccagaac cactaactcc aactatagcc gttttactgt gcgaaggaat tgtacatgaa
     3961 atttcgttca acgttggggc cttcatgcta tacgagaatg aaacgttttt tacagtgata
     4021 tcttgttgaa ataattgttc agaaagcata tattcaaaag actggttcga tttttctgaa
     4081 tcaatcgcga aaatttcatt taaacgatta tttgcaactt gagccgtttg cattttcact
     4141 tgtaaattaa taatgttttg taaaggatct gtcaaaaata ctagtaaagc attgtatgtg
     4201 ataagttgac ctagactaat tctattatcc ataacaaaat aagaacctag ccataaaatt
     4261 aatgcactgc taattaattg aatcaaatgt ttaattcctt gttgaatatt atcaagcgta
     4321 accgtacgaa atgatttttt cattaattta acaaattcat ggtctatacg gttatatact
     4381 ttctcttcac cgctatatgc tttaattgtt tctattcctt taagactttc tatgatacta
     4441 gaatttacag ctgcaccggc tgccatttct tccctgttcg atttttcata gcttttcacg
     4501 aaagcgaata ttgcaactac ataaaaaggc aaagagcata gagtgattaa aaacaacgta
     4561 ctattctgaa tagctaacgt aaccccaaca agcaacacca taccaatatc taaaaatatt
     4621 gataacgtag cacttgctaa agcatcaata atcttactgg catcaagaaa acgagaaata
     4681 atttcccctg attttctcgt agcaaaaaaa ttcattggta aagttaacac atgtttgaaa
     4741 tactgaagca taattgccat actcattcgc tgtcctaaaa taataagaag ataactacga
     4801 ctatattcaa acaatacccg aaataaatag acagcgatca agccaatgga aattatattt
     4861 aaagttgaac gtgcttgatt agggatgaaa aaatcaagaa taccttgaaa ataatatgag
     4921 cttcctatgc caaaaaacgt aatgaaaatc gatgctaaaa cgatgtggaa tactaatcct
     4981 ttttgtttcc aaattatagg caaaaaagaa cttaatccag ctactttttc tttaatagga
     5041 atataagttt ctctaggcat aggtaataaa agaatacctg tccattcttt ttgaaacgct
     5101 tcaatcgttt ttctattttt tccattagcg ggatctgcaa tccatagtac gtttcccttc
     5161 gttttataaa ttacaacgta gtgcatatag ctgttatcaa ccaatatatg tgcaattaat
     5221 ggtaagataa gatctctttc tttccagatc tcactatctg cttgaatagc catacaatca
     5281 aaagacaatt tttcaaatgt ctttttcaat ccaaatgctg aagtaccctc taaatcagtc
     5341 ccagataggt cccgtaattt atgaatcggg atatcagttt taaaatattt taaaatcatc
     5401 gaaaggcaag caacaccaca atctttttca tcttgttgct gcacaaaagg aaatgatttt
     5461 atcacttatc taacacaact ccttctaaaa aatttttata gaaaaattct caatatagct
     5521 ttaatacagt gaaaatattg tctcataatt ccacaaaaac tttgaaaaca gttcttgaac
     5581 actaactttt tcattcctga atcaaattta ttttggaaat tttactattc aagaataaac
     5641 ttgataagtt tcaggaagat ttttactgtt aatcaaatac gtatgttaca gtagcaccgt
     5701 aaaaaatatt atttaaagaa gcgtgtttaa acatgcaaaa tgtaaaggaa ctaagtattg
     5761 tggaaatgca caaactgttg gtggaggcaa taatgcttgg ggaaaattgg ggcaagttgt
     5821 aggtggactt accacagggg cagtaggtgg tgcaggttta ggaacagcaa tttgcggacc
     5881 agcttgcggt gttgttggag ggttatatgg agctgtcgct ggaggtgctg cagctggctg
     5941 ggatgctcga aaaaaataaa aaaggagaat aagtagtatg catattaaaa acacaaaaac
     6001 aacatttata ttatcttcag aagagttgaa aaatattcaa ggtgggagtg ccgtgggagt
     6061 tttaggcact actttttctg gggcaacagc aggagtaaaa ctttgttcag ccggaggacc
     6121 ttatgcaata gctgcttgtg gtgtaggtgg agctttatta ggtgctggat tcagcatgtg
     6181 gaccggtgcg taaacaatta aaaaatatca aacctcaaga ttggtttgtg tcgattacga
     6241 acataggtat gttctttatc ttttcaaaag tttggaatgg acagaactt
//
LOCUS       DAZUMB010000134         6287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000134 DAZUMB010000000
VERSION     DAZUMB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.137"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACG80L_002410"
     CDS             190..690
                     /locus_tag="ACG80L_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5024613.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACG80L_002411"
     CDS             787..2634
                     /locus_tag="ACG80L_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS5024614.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACG80L_002412"
     CDS             2637..3533
                     /locus_tag="ACG80L_002412"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5024615.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACG80L_002413"
     CDS             3583..3972
                     /locus_tag="ACG80L_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5024616.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACG80L_002414"
     CDS             3959..>6287
                     /locus_tag="ACG80L_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5024617.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAZUMB010000135         6263 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000135 DAZUMB010000000
VERSION     DAZUMB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.138"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..327)
                     /gene="rpmB"
                     /locus_tag="ACG80L_002415"
     CDS             complement(139..327)
                     /gene="rpmB"
                     /locus_tag="ACG80L_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS5024618.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            597..959
                     /locus_tag="ACG80L_002416"
     CDS             597..959
                     /locus_tag="ACG80L_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5024619.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1011..2693
                     /locus_tag="ACG80L_002417"
     CDS             1011..2693
                     /locus_tag="ACG80L_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS5024620.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2810..4846
                     /gene="recG"
                     /locus_tag="ACG80L_002418"
     CDS             2810..4846
                     /gene="recG"
                     /locus_tag="ACG80L_002418"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS5024621.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4895..5896
                     /gene="plsX"
                     /locus_tag="ACG80L_002419"
     CDS             4895..5896
                     /gene="plsX"
                     /locus_tag="ACG80L_002419"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS5024622.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6018..6260
                     /gene="acpP"
                     /locus_tag="ACG80L_002420"
     CDS             6018..6260
                     /gene="acpP"
                     /locus_tag="ACG80L_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5024623.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2093 a         1088 c         1425 g         1657 t
ORIGIN      
        1 ttagaataaa taacttcata atttgttcgt ataacaaaaa taaacgcaaa aaaagggtag
       61 gaatcaaaag tcccaccctt aaaaattttt attaaataaa aaaccgttca ctatctgaac
      121 ggtccaagta tcattcaatt aaacgcgttc aacttttcct gatttcaaag cacgagttga
      181 cacccaaact tttttaggtt ttccgtcgac cataacgcga actttttgta agttaggttt
      241 aacagtacgt tttgttgagt tcatagcgtg tgaacggttg tttccacttt ttgtcttgcg
      301 acctgtaaag taacaaactt ttgccatggt aatttcctcc tttgccttga tttggagcaa
      361 tagaataatt cagctcatac cagactaaca tatcatatag aatgggaatt tgcaagtaca
      421 ttttcataga tacaattgac aattgagaag aataattgta aattaaacaa ggttatacct
      481 gaaattttgc aaggtttttg agtactttgg gtaactgcct tttatttcaa gttgcgctat
      541 gataaaatat gatagaagaa aaatgggagt tttttacccg ttaaggaggc tcattcatgg
      601 ctgtaaaaat tcaaacgtca gctggaacca ttgaaattac caatgatgta attgcaacag
      661 ttgttggtgg cgccgcgaca gatgtgttcg gtgttgtagg aatggccagc aaaaatcaaa
      721 ttaaagataa tattactgag attttgcgta aagaaaatta ctcaagagga gtagttgtcc
      781 gccaagaaga taatggtatt gctgtagatg tttatacgat tatcagctat ggtacgaaaa
      841 tctctgaagt ttcccgcaac gtccaagaaa aagtgaaata caatctagag gcattattag
      901 gcgtaactgc caattcagtt aatgtctttg tacaaggtgt tcgtgtactg ccagactagg
      961 caggagactg gctggtaact cagcagtcga tctgaaggag gattatctag gtgagtgtaa
     1021 cagaaataag tgcaagccag ttccaggaaa tggtccaggc aggtgcgaat cgtctgcaag
     1081 taaatgcaga atacgtcaat tcattaaatg tattccctgt gccagatggc gatacgggaa
     1141 caaatatgaa tctatctatg actagtggag ctaaggcagt agtagattca acctcagaaa
     1201 aagtaggaga attagcagca atgctttcaa aaggattgct gatgggtgcg cgcggaaatt
     1261 ccggcgttat tttgtctcaa ttgtttcgtg gattttctaa gcaaattcca gatgttgtct
     1321 cattaaatgc ttctgatctt gctgcagcct tcactcatgg agttgaaact gcgtataaag
     1381 cagtaatgaa accagtagaa ggaacgattt tgacagtagc tagagaagca gcgaaagctg
     1441 gagaaaagaa agcaaaaagt actgatgatg tcgttgaagt aatgacagct gttgtaaaag
     1501 gagcaaagcg tgctttagct aaaactccag acttgcttcc agtcctaaaa gaagtcggtg
     1561 tcgtagacag tggagggcaa ggactgctgt ttgtttatga gggcttttta agtgcattga
     1621 atggagaatt ccaagcggat gaaacatatg aaccttctcc agcagaaatg gatgatatgg
     1681 tcaatgcaga acatcatcgc agtgtccaag gtcaaatggc gacagaagat atccattttg
     1741 gttactgtac agaaatcatg gtaaaaatcg gagaaggacc tacagtcgat agtacattcg
     1801 attacgaaga attccgtaac tatttagatg gtataggaga ttctttactt gtggtcaatg
     1861 atgacgagat catcaaagtc cacgtgcata cagaaaaccc aggagaagtc ttgaattatg
     1921 gacaaaaatt cggttcatta gtcaaaatca aagtcgacaa catgcgcttg cagcatgaga
     1981 cgattttgga acacgatgaa gaagtgaacg agtttgcgaa aaaagaaata ccacgtatcc
     2041 cgtatggtat cgtagcgatc gctgctggta aaggcgtcca agagctgttt gaaagtttag
     2101 gcgctaatta tgttatcagt ggtggacaaa caatgaatcc aagtacggaa gacattgtta
     2161 aagcgattca agaagtaaac gcagataaag tgattgtctt gccaaacaat aagaacatct
     2221 tcatggcagc tgatcaagca gcagaagtag cagatatccc agtagcagtt gttccttcaa
     2281 gaacgatttc ccaaggattg acagctatgc tttcattcaa tgatcaagct tctttagaag
     2341 aaaacaaaga aacgatgaca gaaatgttgt ctagcgttgt gagcggtcaa gtcactcacg
     2401 cgatcagaga tacctcaatt gacggtgtag agatcaaaga aggagacttt ttgggcatga
     2461 tcgatggaaa aatcctgatc tctaacccag atatcacagc aacagcgtta agtacactag
     2521 aacaaatgat caatgaggac acagagatcg taacgatctt ggcaggtgat gaaggaacag
     2581 ttgaacaagc aaatgaattt gctgagaaac taacagaaaa acatcctgat ttggaaatcg
     2641 agatccacca aggagatcag cctgtctatc cttatctatt ttctgctgaa taatttctgt
     2701 gtcagaatat ctttctaaca cagtattcat tcataaaagg ccttcggtta gtgatcaggt
     2761 atgatcgctg accaagtcct ttttttgtta aaggagggag gaaaaattta tgcgttcttt
     2821 agatgatcca atcactatgc tggcaggagt cggagaaaaa agagcagcta gtcttgccag
     2881 cttgggcatc caaacgattg aagacttact gacttattat ccttttcgct acgaagatat
     2941 tcaggaaaga aatctaaacg agatccagga tcaagaaaag gttactttga aaggaatcgt
     3001 agtctcgcct cctgtgatga atcgatttgg ttataaaaaa agccgattgc aatttcgtat
     3061 gatgcaagaa catgatgtgt tcaacgtttc tttttttaat caaccttatc taaaagaaaa
     3121 agtcatttta tcggaggaaa tcgccgtata cggtaaatgg gatgcaaagc gtaaagcctt
     3181 gaacgggatg aagattttag gttctcaaac aatggatgac ttttcgccga tatatcacgt
     3241 caacaaatca attcgtcaaa cgacattagt tgatctaatc aaaaaagggt ttcaagaata
     3301 cggcacattg attgaagaaa atctgccaaa caaccttgtg gagaaatatc gactgcttga
     3361 ccgagcttct gctgtaagaa gtatgcattt tccaaaagac cacgaagaaa accatcaagc
     3421 caaacgacgt gtcgtttttg aagagttctt tttatttcaa atgcgtcttc aaggattgaa
     3481 aaaagcagaa aaagcagaaa cgaatggttt ggagatcctt tatgatgtcc aaaaattgaa
     3541 acaatttact caaaagctac cttttgaact gacaaatgca caaaaacgtg taacgaatga
     3601 aatctgtcgg gatcttcgca gtccgcagca tatgcagcga cttttgcagg gagatgtagg
     3661 tagcggaaaa acagtggttg ccgcgattgc gttatacgct accgttacag caggtttcca
     3721 aggtgcgttg atggtgccta cagaaatctt ggcacaacaa catatggaaa gcttgcaaca
     3781 gctgtttgat cccaatgaag taaccatcgc attgattacg gggtcaacga aagcaaaaga
     3841 acgacgtgag ctccttgaaa aactagagca aggggaaatt aacgtcgtga tcggcacaca
     3901 tgcattgatc caagatggag tcgaatttca gaatttaggt ctagttatta cggatgagca
     3961 gcaccgtttt ggcgtgaacc agcggaaagt acttcgtgaa aaaggctggc gaccagatgt
     4021 cttatttatg acagcgactc cgattcctag aacattagct attacagcat atggtgagat
     4081 ggatgtatct atcattgatg aacttccagc tggacgtatc ccgatcgaaa cacgctgggt
     4141 aagaacacct caattggaca gtgtactgga ttggacgaaa aaagaactgg caagaggaca
     4201 tcaaatgtat gtcatctgtc cgctgattga agaatcagaa gcattagatg taaaaaatgc
     4261 agtggaaatc tatgaaaaac ttcgtgaact atttgcacca aaatatgaag ttggcttgtt
     4321 acatggtaaa atgaagaatc aggaaaaaga agtaatcatg gaagccttca aagacaacca
     4381 gatgcagata ttagtttcga ctactgtaat tgaagtaggg gtaaacgtgc cgaacgcgac
     4441 cgtcatgctg atcatggatg cggatcgatt tggattggca cagctgcacc aacttcgtgg
     4501 acgtgttgga cgcggaagtg atgcttccta ctgtatctta gttgctaatc ctaaaaatga
     4561 actaggtgtg gaacggatga aaatcatgac agagaccacc aatggttttg tactaagcga
     4621 aaaagatctg gaattacgtg gtcctggtga agtatttggc ttccgtcaat ctggtctgcc
     4681 tcaatttgcg attgctgact tggtgacgga tggaaatgtt ttggaggtag ccagagaaga
     4741 ggctgcagcc ctttggcaaa taaaggattg gcagatgctt cctgagtatc agcctttagc
     4801 gcaaagcctc caaatagaaa atcaggataa tcgttatttc gattagcaga ctgtgctata
     4861 ctttatactg atgacggaaa aaggagtgga aacaatgaaa attgccgtag atgcaatggg
     4921 cggagatcac gcacctcaag caattgtaga aggcgtggct ttagcgcagc aagattttcc
     4981 agaagtagaa ttcttacttt acggaaaaga agcagaaatc aagaaatact taacaaatga
     5041 aaaaaacatc acgattatcc atacagacga aaaaatcaat agtgatgatg agccagtaaa
     5101 agcaattcga cgaaaaaaga ctgcatcaat ggtccttgct gcacaagcag tgaaaaacgg
     5161 tgaagcagat gctgtatttt cagcagggaa tacaggcgcg ctactagcag cagggttgtt
     5221 catcgtagga cgtatcaaaa atatcgaacg ccctggcttg atgtctacct tgccagtagt
     5281 agggaaagaa ggtgccggtt tcgatatgct ggatcttggc gctaacgcag aaaacaaacc
     5341 agaacatttg ttgcaatacg gtattttagg ttctttttat gcacgtaaag tccgaggtat
     5401 cggacgtcct cgtgtcgcac tcttaaacaa cggaacagaa gcaacaaaag gtagtgaagt
     5461 gacgaaaaaa gcctatgaac tccttcagaa tgaaacaagc ttgaatttta ttggaaacgt
     5521 agaagctcgt gagctactca atggtgtggc tgatgtagtg gtcacagatg gatttacagg
     5581 caacgctgtt ttgaaatcta ttgaaggaac agcaatgaat atgatgaatc tattaaaatc
     5641 tgctattcta aatgaaggga tcaaaggaaa aatgggcgct atgcttttaa aagacggctt
     5701 acgttctttg aaagcagaaa tggattattc caaacatggc ggtgccgttt tgtttggctt
     5761 gaaagcacca gtcattaaaa ctcatggagc aacaggcccc gatgctgttc gctatacgat
     5821 ccgccaaatc cacacaatgc ttgagacaga tgtagttgga caattagtcg aacaatttga
     5881 aaaaagcgaa gaatagtatt tggatatctg aagcgatccg ttccattgaa aaaaaggtgg
     5941 acattcctga aaaatgccag taaaattagc catggtgtag gtaattgaca agtgtatgat
     6001 tcttggaggt gaaaaagttg actcgcgaag aagtatttaa caaagtagcg aaaattattt
     6061 caaatcattt tgagatagat accgataaag tgacggatga attgaacatc aaagatgatt
     6121 taaaagctga ttcaattagt attatggaat tcgttttaga gcttgaagat gaattcggta
     6181 cggaaatttc agatgaagac gctgaacaaa ttgaaacagt cggtggcgca gtagattata
     6241 tcagcagcca tcttaaataa ttt
//
LOCUS       DAZUMB010000136         6110 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000136 DAZUMB010000000
VERSION     DAZUMB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.139"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(322..807)
                     /locus_tag="ACG80L_002421"
     CDS             complement(322..807)
                     /locus_tag="ACG80L_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024624.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(946..1161)
                     /locus_tag="ACG80L_002422"
     CDS             complement(946..1161)
                     /locus_tag="ACG80L_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS5024625.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1385..2185
                     /locus_tag="ACG80L_002423"
     CDS             1385..2185
                     /locus_tag="ACG80L_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5024626.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2204..4072
                     /locus_tag="ACG80L_002424"
     CDS             2204..4072
                     /locus_tag="ACG80L_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5024627.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4065..4556
                     /locus_tag="ACG80L_002425"
     CDS             4065..4556
                     /locus_tag="ACG80L_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS5024628.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4544..5098
                     /locus_tag="ACG80L_002426"
     CDS             4544..5098
                     /locus_tag="ACG80L_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS5024629.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5116..>6110
                     /locus_tag="ACG80L_002427"
     CDS             5116..>6110
                     /locus_tag="ACG80L_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS5024630.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2162 a          968 c         1223 g         1757 t
ORIGIN      
        1 tttgaaaaag ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa
       61 ttcttctatc caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt
      121 aactcagttg accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa
      181 gtgagtatat tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat
      241 ttatactccg tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta
      301 aatccgtata atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga
      361 tccttcgggt gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg
      421 tttgctggaa gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat
      481 atcgtcactt tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt
      541 ttctaatgga agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa
      601 cgactgaata cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat
      661 ggaagtagta gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga
      721 ttctttaaca gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa
      781 caagcctgcg taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc
      841 agtatatcac ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa
      901 gggaaggttc atgaagataa taactttttt aataattata gctttctaat atctttcatc
      961 aacttttcta gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac
     1021 gatataaatg atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc
     1081 aagttcgaac aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc
     1141 aatagcatct aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat
     1201 caaaaagaga aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat
     1261 gaaaattttt tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg
     1321 tcttaatttt ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc
     1381 acatatgtcg gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc
     1441 aggaattgga agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt
     1501 tgatgtaact gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc
     1561 ttctaaaacg gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga
     1621 tggtctagtc aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc
     1681 aaaaagcaag tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa
     1741 aagtgtatat ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt
     1801 gatcgtgaat ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc
     1861 tgctacaaaa gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag
     1921 gaatgttcgt gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca
     1981 agaatatgaa gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg
     2041 ttacagtcga acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga
     2101 tctagtctgc tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat
     2161 ttcaggaggt aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag
     2221 atggtaccaa ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca
     2281 aaaagaaatc aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga
     2341 aattgccggt attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga
     2401 ttttgatttt tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc
     2461 ggcatccaaa agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg
     2521 tatggatgat ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa
     2581 gacattaaaa gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg
     2641 actaagaata ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc
     2701 ctacttttca gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt
     2761 tcagaagaag acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat
     2821 ttctttgaat agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg
     2881 gtgttttgaa tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga
     2941 actaactttg agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac
     3001 aagtataacg atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat
     3061 gatgcgtatt cgacgattag atctcattga tctggatgag tccttcttat atcaagagat
     3121 gaaacagcac ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt
     3181 attttatggt gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat
     3241 acaagcatta gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac
     3301 gttgtatttg gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc
     3361 cattgttcat acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat
     3421 attggggagg gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct
     3481 aaacaaatat ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc
     3541 ggtgatccat ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc
     3601 aaaaaaaaag cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga
     3661 aaaaaagacg tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta
     3721 tgtggatgaa gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt
     3781 taacaaagaa tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc
     3841 agtagggcta ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt
     3901 atttgccata cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga
     3961 tatcctattt gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca
     4021 agaatcattt ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc
     4081 tattcatagt tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac
     4141 taaagcattt caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta
     4201 aacgacctgg gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc
     4261 aagttttaga agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac
     4321 ctgtctatgc gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg
     4381 aagatcgttt gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg
     4441 caggaaatga aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc
     4501 ggatatggaa aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa
     4561 atttgctgag ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat
     4621 gacaggaatc gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt
     4681 tttaggtgaa gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg
     4741 ttaccttgtg ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat
     4801 ggctcgtttc cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg
     4861 tcatacgagc aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg
     4921 gattgctcaa gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt
     4981 gcttgtggga ctgctgatga actttattgg tggttggata actgatttta ctactggatt
     5041 tgtatgcaga cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag
     5101 aaaggagagt tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga
     5161 ggtcctttga ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga
     5221 ggtgagaggc caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat
     5281 gggttcaaaa catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg
     5341 actcttagat gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca
     5401 ggaaaaagtg gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg
     5461 ttagaacaaa tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca
     5521 tctccagata aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag
     5581 cagcaaaact ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat
     5641 caatcagcaa gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc
     5701 gtttcccttt tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt
     5761 atggcacctt tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg
     5821 ccatttttat ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg
     5881 atcggtgtag agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt
     5941 gctatcaata ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca
     6001 aaagctgaga cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc
     6061 atcccacgag ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAZUMB010000137         6002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000137 DAZUMB010000000
VERSION     DAZUMB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.140"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG80L_002428"
     CDS             <1..395
                     /locus_tag="ACG80L_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5024631.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACG80L_002429"
     CDS             398..1228
                     /locus_tag="ACG80L_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS5024632.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACG80L_002430"
     CDS             1244..2095
                     /locus_tag="ACG80L_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5024633.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACG80L_002431"
     CDS             2092..2451
                     /locus_tag="ACG80L_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS5024634.1"
                     /translation="MNKYRNKKTVHRGIKSDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACG80L_002432"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACG80L_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACG80L_002433"
     CDS             2624..2929
                     /locus_tag="ACG80L_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024635.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACG80L_002434"
     CDS             2929..3240
                     /locus_tag="ACG80L_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS5024636.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACG80L_002435"
     CDS             3237..3410
                     /locus_tag="ACG80L_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024637.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACG80L_002436"
     CDS             3407..3823
                     /locus_tag="ACG80L_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5024638.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACG80L_002437"
     CDS             3823..4491
                     /locus_tag="ACG80L_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS5024639.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACG80L_002438"
     CDS             4488..4865
                     /locus_tag="ACG80L_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024640.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACG80L_002439"
     CDS             4862..5161
                     /locus_tag="ACG80L_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024641.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACG80L_002440"
     CDS             5151..5309
                     /locus_tag="ACG80L_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024642.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACG80L_002441"
     CDS             5403..5819
                     /locus_tag="ACG80L_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5024643.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          900 c         1391 g         1480 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtctg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAZUMB010000138         5787 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000138 DAZUMB010000000
VERSION     DAZUMB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.141"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..1226)
                     /locus_tag="ACG80L_002442"
     CDS             complement(96..1226)
                     /locus_tag="ACG80L_002442"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS5024644.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1285..1512)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1874..3076)
                     /locus_tag="ACG80L_002443"
     CDS             complement(1874..3076)
                     /locus_tag="ACG80L_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5024645.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3136..3339)
                     /locus_tag="ACG80L_002444"
     CDS             complement(3136..3339)
                     /locus_tag="ACG80L_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5024646.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3481..4548)
                     /locus_tag="ACG80L_002445"
     CDS             complement(3481..4548)
                     /locus_tag="ACG80L_002445"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5024647.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4606..4713)
                     /locus_tag="ACG80L_002446"
     CDS             complement(4606..4713)
                     /locus_tag="ACG80L_002446"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024648.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4876..5688
                     /locus_tag="ACG80L_002447"
     CDS             4876..5688
                     /locus_tag="ACG80L_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024649.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1714 a         1189 c          921 g         1963 t
ORIGIN      
        1 taaataatat aaaaggtaaa taaaaaagag tggatttctc cactccatga aaagccgact
       61 ttttgggacg cttttttttg ccaagcctca catttttata gccaaacttc ttttgcgact
      121 tgctgcaata attggatttt cttccattgt tcttcttctg ttaaatggtt tccttcttct
      181 gttgaagcaa acccacattg aggactgata cttagatttt ccaatggtac aaattgttgt
      241 gcttctttta ttcgttcttt tattttctcc gtatcttcca aatctggaaa cttagaagta
      301 actaatccta aaacaacttt actttctttg ttactccaat gtcttaaagg agtaaaatct
      361 cctgctcggt cagaatcgta ttccaaaaag aaaccatcat aatttgttga aaacaactct
      421 tctgcaatgg gttcatatcc accggaaaac agccaagaag aagcgtgatt cccgcggcag
      481 atatgtgttg tgatcgtcaa atcttctggt ttgtcttcca gtgcgttatt gataatctct
      541 gtacaaatat gggccatttc tttcggatca attccttttt gaagaagtgc ttctctttta
      601 gtttgatcag ctagaaacgc ccaatttgta tcatcgaatt gcaggtaacg gcaacctttt
      661 tcataaaaag catgaatcgc tgctttgtaa gtagaggaca agtcctcata aaaatcagat
      721 agatcaggat aaatctcctt gatttttgaa gaaccatcat tagataggat ctcttgtcgt
      781 aaaatcatcg tgggactagg gatcgttgct tttgcaacaa aagtatcatc tgtttccact
      841 acttctttca gaaaagcaaa atcttttaaa aatgggtgat ttggattgaa agataattta
      901 tctactaccc gaatattata agctggtgct gcttttccgt ggaatgtttg attatatcca
      961 gtttctggaa catagccttc gataccattt agttcttcta aaaaatcaag atgccaaaat
     1021 ccacgccgaa attcaccatc tgtaattcct ttgatgccga ctgctttttg gtcttccact
     1081 aattttttta tttcctggtc ttcgattgca cgaagttctt cttttgaaag taagccttct
     1141 tgaacttttt ttcttgcttc tttgatcgat tgcgggcgta agaaacttcc gacatgatct
     1201 actgtaaatg gtctattttt gctcatttca ctcactcttt tctttgtttt ttgtaaaaaa
     1261 aatcctttta ctagcaaata ctagtaaaag gacgattata ctcgtggtac cacctttttt
     1321 cagaaaaaac attctttctc tcagcctgac aataaaatca gtggtcttgg taacgaaaga
     1381 ctgttcgttt ttgcctacct ttcgacaaaa aatcctctaa ggccattttc gacagtcaac
     1441 ttctagcttt ttacaccaac caaagcctct cttgaaccat tttctgtcta ctttccttct
     1501 catcggatac tattagaata aaattagaat agcatgtttg aaagaaaagt caatagggaa
     1561 taagaacaaa caaaaactaa tttgttattc ttagtaattt gtttgtggaa tacaataaag
     1621 cttttctaat atgataattg ttcagtcgtc ctttcatctt agatcacttc cttaaaatga
     1681 tcatacccgt ttcaaccaca taaaaaagga cttaattttg aaaaatttcc catcaaaact
     1741 aagtcctttt tattatagtt atgcaaattt gcttattttc tcgctatttc cgatacatct
     1801 tgaacaggag caattcgact gaaaacactg gtgcaaaagc atttatttgt cttatggtag
     1861 aaccgtggaa aactcagact ggtaataaat tgtcattttt tgaaagaata gcgccctctt
     1921 gaacattatc aatcgcactt accacttgtt gatctgactt tgcatataag tgaccgtaag
     1981 ttcctaaagt gatttgaata ttcgcatggc ccaatcgttc ttgaatagat agcggttgaa
     2041 tattctgctc aattagaaat gcaacatgag aatgtcgtag cgcgtgaagc ttaatcggtt
     2101 tgacacccgc ttttttagca gcttctttta accagttcgc aaaagaataa ggtggcgtaa
     2161 atgttccgtc caactgagca acatattcaa aatttccaat acgtttttgc aagtcatacc
     2221 actgtctgag ttttaaggaa agcttcttcc ccaacccaat cgttcgataa gcacatttag
     2281 ttttgggagt cgaaaagtac caatcatctt ttgtattata aatcaatgtc ttctcaatcg
     2341 ttatcgtgtt tttctcaaaa tctacattcg accaagacaa agcttcaagt tctccaatcc
     2401 gaatgccagt ataaaagagc atttcataca caatcttccg atggtgggtt tgaatcttat
     2461 catccttcaa ttcattcatc actttttgaa actcactgtg ttcccaaaac tcaactttct
     2521 tattctcaga aggaagcgaa gaaacatctt caacgggatt tttagcaatc accccctctt
     2581 taacagcccg atccaataca atttttaggt gccctcgaat ccgattgata taagattttg
     2641 ataacgtttc gccattaact tttttccgtg aattacgtga ctctgtctgg cttagttcaa
     2701 ataaccaatc ctcaatatcg tgacttgtaa tatctcgaac acgaaaatga tagaaaaact
     2761 gcatacgctt ttcgaaaatg aattgcgcat tctcatatgt ttgacttttg acctgtctct
     2821 cgtaccattt tacaaaccta tccttgtaaa actcttcgaa agtcatttga ttggtagaac
     2881 acagttgatt aggaattgtc ttgttaaaat catgtgtttc catgtacttg tcataagcaa
     2941 ttttggcatc tttttgtgaa gcataacctc gtttagtctt ctgaaccttt cttccatttt
     3001 catctatacc tagatacgct cggaagaacc atttttttgt ctttttatct tggtaaatat
     3061 ttttaatttt tggcatcgtt ccattcctcc ctgtacttga gaaaagctac tttaatagta
     3121 ccgcgcttgt actgtttatt ccgcaacttg aatgtgaaaa agatgttcaa ttactcggga
     3181 tggcaccaca ccaacttggc gattattata aaaatcaata ccttcaatcc ttgcaagata
     3241 tgccttgctc tctttaatca ttcgtgcagc ttgactaggt ttaaagccca tctctatcaa
     3301 aattgcacga tttaccatga aatcacattt tgtttccatt ctctcacctc ctgattcttt
     3361 actcctttta aaataatcct ctttataatc tattgtttct cattcgatgg ttgtgttata
     3421 ctcgtcttgt cgaggcggag tacgttgcac aacttgttgt gtaagcagta ctttgctttt
     3481 ttagtagctt gttttctcat ttgatatact ttgtaatttt cgttcataat actcaacctc
     3541 ctcctgataa tactccgggt ctgccttcat attttggaga agtttgatct gtcttggatt
     3601 catttctcct tgttcaataa tcaaaggcaa caaggaagaa tgaaacgttt gatcgacctg
     3661 tgcaataaaa actaatgatg gtgaaacacc atgaattaac caactcatag ttttctcaaa
     3721 actttgagga attgtatcta aagaaatggt gattgcgcca taattgctaa taaaggctgc
     3781 ccatttggca tcaacttttg cacctagatc atttggatca cggtcataaa aacaaattgc
     3841 tccattaagt aactcaaaga acagtttggt taaatcatgg gtttttgaaa taatctttgc
     3901 caagctttgc gctttctctt ttcgataccg tagttcaaag cgattcttta cttttgcctc
     3961 ttctaaagga attcctcgtc gcttccgttg ttcatggtcc ttctgataaa agcaaaaacg
     4021 acactgactc ttctttgagc ctaaatacaa ggtctcacca tttgattcat tgtttatgat
     4081 atccgtaccg ccatgagatt cactcacttg aaaagtggtc cacacatggc ctagtattac
     4141 cttatgctta agttctggaa tgctgagact ccccacaaaa tcatctaaag taaaatcaat
     4201 ccgtgtaaag ttcccctgat aatctaatac cttacgaaaa aactgttgcc aatttgtttt
     4261 tcgtgttttt agccatcttg ctaagtgttt acagccttga cctgaaaact ccatcacagt
     4321 tcctttaacc gcatcgtcaa tggaataccg aatcgtgatt acttgattcc acacgtattg
     4381 gccaatatag ccctttggcg cataatccag ttcctcaaaa cgttcaatgg gcatttcaat
     4441 aatctcttca attagcttta aatgttgatt cgagaaaaag tgaatggtta gataatcaat
     4501 caaaacacgg ttaactggaa cgacctcttc ttgcaaacta tccaacacaa gattcaattt
     4561 ttcttcactt aattgtctcg ttcctttttc tactttcgat aataactatc tcgaaatccc
     4621 cactttttct gctaaatatc gttgtgtcca ccccctttct tctcgagaaa ctcgaatggc
     4681 ttcaccgaga tcatgttgat taacttggtt catcgacaaa cctccttctg ctatttactt
     4741 gtcaaagaac ctattgaact tattttaagt acatttttat tgtacgcctg tatttatttt
     4801 aagtcaatat gttttgactt tttttaagta cacacgtata ctaaatttaa gaacttatcc
     4861 tgaggaggag ttttcatgga gatagacaag caagcagtcg gcaatcgaat tcggcagatt
     4921 cgccaagaat taaagttgag tatggagaaa tttggcaaac taattggtga tttaccgcga
     4981 agtacagtca ataactggga acgtgggatc aaccttccaa aaaccgaaac ccttcaccaa
     5041 atcgcagaga taggtcatgt caccaatgaa tatcttttgt atggtgatca agagaatcaa
     5101 tacattttag aaatgctcca aaaaaaggca ggtaagcttg atcctaagat tgaaggtctc
     5161 atagtagacg aaatcaaaca cgctgacctt gttagtgaaa aagaaatgga tcgaatgatt
     5221 gaatttttta ttacaaatct cattccacca actgaacaag accagtttac tttccaatgt
     5281 atcgatgaaa aaaagcagct ttatcttggt tcaactaatt ttggtaagga agcccagctc
     5341 tatttgcaac atgataatca aaatcatatt ctgcacatga tgccctttac cttctccaac
     5401 tttagtgtgg atcgcatact cgtctattta gcaaatcaaa aatcctattc ctattttggg
     5461 aaacatttac ccaaggaact aggtgagaaa gccatccttc tatactcaat taatcaattg
     5521 accagcgatg tgcgaattgc cccgttggtc tattcagaag aaaccgcttc ttatcagtac
     5581 acagaggaca atcaatatct actagaacag caatatcttt atctaccatt cgtgatggaa
     5641 gtcgagaaag aacgcctgtt aaatgcagcg tatccatcat caaaatgagg tgacaaaatt
     5701 gataagcacg tgacaaagaa aatactgcta ggacactccc caatgggtag ttgtcacagt
     5761 ttcctgttct ttgttagccc cctgtta
//
LOCUS       DAZUMB010000139         5771 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000139 DAZUMB010000000
VERSION     DAZUMB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.142"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            284..1276
                     /locus_tag="ACG80L_002448"
     CDS             284..1276
                     /locus_tag="ACG80L_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5024650.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1336..2757
                     /locus_tag="ACG80L_002449"
     CDS             1336..2757
                     /locus_tag="ACG80L_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS5024651.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2750..4249
                     /locus_tag="ACG80L_002450"
     CDS             2750..4249
                     /locus_tag="ACG80L_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS5024652.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4288..5682
                     /gene="uxaC"
                     /locus_tag="ACG80L_002451"
     CDS             4288..5682
                     /gene="uxaC"
                     /locus_tag="ACG80L_002451"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5024653.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1895 a         1069 c         1170 g         1637 t
ORIGIN      
        1 ctaataaaaa catcggtaaa aatattttat aaaatactgt acttttcttc attttcttcc
       61 tctcctcctt ttaaaataaa aggctttcat ttttctgtaa aattgatact catctaatta
      121 aatgagcatt ccccgctttc aagaatagca taattaaaaa taaaaatcca agtaattttt
      181 aacgtgtgca atcttttcat atcaaagatc tattgactta tcgtagttat cgtaatatga
      241 tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa cgatcaaaga
      301 tatcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga ataacagcag
      361 tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa tgaattattc
      421 gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg gactattttt
      481 ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg caggcatcca
      541 ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg ttgatttaac
      601 agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg atgatgatca
      661 gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca atcgtcacat
      721 caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta tgcaggcagt
      781 agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa agaaaaattt
      841 tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag cgagcatccc
      901 tgtcaatcta gcctattttt taacaggaga ttacagctta gaaagcggcc tccaaaacat
      961 ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca acgacgacat
     1021 agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc agaacatttc
     1081 tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc ttaccaccgt
     1141 acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag atttgatcga
     1201 aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc ttcgtgagtc
     1261 gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg tgcataacac
     1321 agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa cgaagcacct
     1381 gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga ttggcagatt
     1441 cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca acctttagaa
     1501 caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat tttacaagga
     1561 ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga acaaatgatc
     1621 aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga cttggcctat
     1681 atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa tttaacagat
     1741 actcccccac agagcttccc gggcaaactg acagcctttt tataccgacg tttcaaatta
     1801 aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg cgaacgtcta
     1861 aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt tgttgagtgg
     1921 ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc aggttatcct
     1981 cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt gatggtcaaa
     2041 gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa tcaactacca
     2101 ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta tcgtgaacgt
     2161 aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt gttggcggaa
     2221 ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt tgatcagtta
     2281 ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc catttatgct
     2341 gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga agcgatttca
     2401 ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa gtatatcgaa
     2461 gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact tcttatgtat
     2521 cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt tactgatgct
     2581 tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct ttggggagaa
     2641 gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc aaagatcgat
     2701 caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca tgcttgaaac
     2761 cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat taacccctat
     2821 tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg aagatatccc
     2881 gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga tcaaatatgg
     2941 ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc acactcataa
     3001 tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg tccatccaag
     3061 tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg gaaaagtagg
     3121 gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca ttgctgaact
     3181 gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc atattacgat
     3241 tttaaagcat ccttatggct gttcgcagct tggaaaagat catcaaaata ctcgagaaat
     3301 tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg gattaggttg
     3361 tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg aagaacgagt
     3421 gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg ctctattaga
     3481 agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct ctaagttgaa
     3541 tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta atcctttact
     3601 tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa cagaagtgcc
     3661 agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag aagtttttga
     3721 ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg gggaaccagt
     3781 ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag aagataagtc
     3841 tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac aatatggcga
     3901 aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc ttgtagctgc
     3961 ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa gagggactcc
     4021 gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt ttgaacgcaa
     4081 aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg aagtaatctt
     4141 agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga aaaatgaaca
     4201 aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat tttcataaaa
     4261 cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt attaaaagac
     4321 gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat tgattatcac
     4381 tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct gacagaagct
     4441 tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt gcctgaagaa
     4501 aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca aaccgtacca
     4561 aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg tttcttcggt
     4621 attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc aaatgaaatt
     4681 ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt agaagtgatt
     4741 tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc tcatgaagaa
     4801 ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat tgaaaaagaa
     4861 ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat caccacctat
     4921 caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca tggatgtcgt
     4981 gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac agtattagaa
     5041 gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat cgatgcttat
     5101 cttacagaaa cactgataca tttgacccac ttctatcatt ctcataattg gacgatgcag
     5161 ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt agggcccgat
     5221 accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa attattgaac
     5281 cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa tccgaatgac
     5341 tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg aaaactgcag
     5401 ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca attgacacaa
     5461 tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc aagaagtttc
     5521 ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt aggcgaaatg
     5581 gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt tcaagcgatt
     5641 agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa tagtaacagt
     5701 aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt ttagagaaga
     5761 agacggacca a
//
LOCUS       DAZUMB010000140         5614 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000140 DAZUMB010000000
VERSION     DAZUMB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.143"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..2008)
                     /locus_tag="ACG80L_002452"
     CDS             complement(59..2008)
                     /locus_tag="ACG80L_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5024654.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="ACG80L_002453"
     CDS             complement(2050..3108)
                     /locus_tag="ACG80L_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS5024655.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="ACG80L_002454"
     CDS             complement(3227..3682)
                     /locus_tag="ACG80L_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024656.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="ACG80L_002455"
     CDS             complement(4266..5069)
                     /locus_tag="ACG80L_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5024657.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DAZUMB010000141         5610 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000141 DAZUMB010000000
VERSION     DAZUMB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.145"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..474)
                     /locus_tag="ACG80L_002456"
     CDS             complement(16..474)
                     /locus_tag="ACG80L_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5024658.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(487..1818)
                     /locus_tag="ACG80L_002457"
     CDS             complement(487..1818)
                     /locus_tag="ACG80L_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS5024659.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1829..3271)
                     /locus_tag="ACG80L_002458"
     CDS             complement(1829..3271)
                     /locus_tag="ACG80L_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5024660.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3258..4022)
                     /locus_tag="ACG80L_002459"
     CDS             complement(3258..4022)
                     /locus_tag="ACG80L_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS5024661.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4126..5001)
                     /locus_tag="ACG80L_002460"
     CDS             complement(4126..5001)
                     /locus_tag="ACG80L_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5024662.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5256..5340)
                     /locus_tag="ACG80L_002461"
     tRNA            complement(5256..5340)
                     /locus_tag="ACG80L_002461"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5304..5306),aa:Leu,seq:caa)
     gene            complement(5358..5429)
                     /locus_tag="ACG80L_002462"
     tRNA            complement(5358..5429)
                     /locus_tag="ACG80L_002462"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5395..5397),aa:Gln,seq:ttg)
     gene            complement(5439..5511)
                     /locus_tag="ACG80L_002463"
     tRNA            complement(5439..5511)
                     /locus_tag="ACG80L_002463"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5476..5478),aa:His,seq:gtg)
BASE COUNT         1702 a         1198 c          854 g         1856 t
ORIGIN      
        1 ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg
       61 ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg
      121 tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg
      181 gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat
      241 atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa
      301 tatattatcg attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc
      361 gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat
      421 aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta
      481 tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat
      541 tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt
      601 ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctactgcaa cttgtgcctc
      661 catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattggct ccacaccagt
      721 tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc
      781 ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat
      841 atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc
      901 gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt
      961 agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc
     1021 taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa
     1081 ggttttttgc caagttttat ctcctatatt aaaatcggcg acttgcattt cttttgttat
     1141 tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt
     1201 ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc
     1261 gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt
     1321 tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt
     1381 caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt
     1441 gattgagcgc atcccataag caaatcctcc gagtccacac gtttcttgtc ctactaatcc
     1501 atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg
     1561 tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg
     1621 aatatttgaa aaaccttttc ttttcagcat aaatttaaca atcaagtaca tatccatatt
     1681 tctttcttca tcaatatcat ataaacgtat ttcagataat ggcaaccgtt tttcattgtt
     1741 caaaatggct tgaacgattc ctggtgtata cgtactcccg cctccagcta tagtgataat
     1801 ctgttttttc atcatcatct tttcctttct attcttctaa taatttattg aattctgcca
     1861 taacataagt cacttctaag ccgatgataa tttgataatt tttttcagtt ggtttaatga
     1921 tcccgcttat ccctaattgc ttgattcgct gttcctcgac catggtggga tccactacct
     1981 ctacccgtag gcgtgtatta caactataga catccaggat attggctttt cctccaaacg
     2041 cttgtagcat ttttgttgcc atatattcat aatttttact tgctaaccct aactctgttt
     2101 cttctaaggt tacttcattg ccaaactctg ccttagcttc acgtcctaat gtctgaatgt
     2161 ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga
     2221 ttggaagaat caaaatagca ttggtagcga ttcgaaaatt gatgagataa tcaacaatac
     2281 atgctccgaa tgtaaaacca attcgtatat tgaataagta acagacaatc cctgctaaac
     2341 cggcaaaaag tgcatggatc acgtacaaca taggagaaag aaacatgaaa ctaaattcaa
     2401 caggctcagt gatatttgca ataaatgaag ttcctgcacc gcttcccatg attcctttga
     2461 ccatctcttt attttcttta taagctgctt tatagatagc tagacatata gctggtatac
     2521 caaacatcat tactacaaaa aaaactgata aaaacagtcc agcctgagga tctccgttga
     2581 taaagcgtgt catctctcct ctgaaaactt ccttgctagg agatgtatag cttcctaaat
     2641 cgtaataaat atacgtattc aacacatggt gcagtccaaa ggggatcagc aaacgattca
     2701 aaaacataaa tatccccaca cctaatgctc ccatatcaac gagcagtttt gcaaaattat
     2761 caatcccaat ttggatgagc ggccagataa agcccaaaaa aacagctgcg attgtttgta
     2821 gaatcatcac catagtcaat gggaattttt cgcccgcaaa ataagaaaaa actactggta
     2881 atttttgttc tttaaagcga ttatacgtcc atgccgctac taaaccggaa ataattccac
     2941 caaaaatccc catattcact tctgggtcca taatttctaa gccttttttc aaggtagcaa
     3001 tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt atttttgaca aaaccgattg
     3061 caactcctat agcaaataag atatccaagt ttccaaaaac ggcatttcca gctgcaccaa
     3121 ttactgggat gttcagcata tcgtcagctg ataaacgcat gatcaatccc gctaatggca
     3181 tcgctgcaac tggaagtagc atcgctcttc caagcttctg cacatttgat ttaacattca
     3241 tttttattcc tcctctatta aatatcctca tatgttacta actcaatcaa aggatcattt
     3301 ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt acgaggatac
     3361 acaaaacttg aaccatttaa caattcctga tccaaatacg ctgcgtgaca catgacttcg
     3421 atcgttttat cttctctcaa tatcatgagt aaacttgcca aatattcttc cacactcgta
     3481 ttgatttgtc gttttttttc atcagtttct cctacgacat cgaaacaagg ctcaaaataa
     3541 tcgacatgac gaacttcatc ttttcgttca aagttgctcc gaaccggcaa atcatatttt
     3601 tttgctaatg taacaactac ctcctgattt tcatccaaag tatgagtatg atgatgactg
     3661 tctaaatgag ttggtatgat ccccgcatga taaatttttt gaatctgagc attccattcc
     3721 tgatacagtt gatctttgtc aatgttaaat ggatgctgat aataagataa ggatctgaac
     3781 tctccgcttt cgataagagt atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt
     3841 aaatggactc ctacacctag cctaggcatt tcctttctta gtttcacagc atgctcgaaa
     3901 cccggcatat ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata agcatccata
     3961 atcccataat tcacaccatg gctatagcca aaatcatccg aattaaaaat cacttttccc
     4021 atttcattct ccttcttttt tctactaaaa taggcgcaac caaataatca tagtccattt
     4081 actatgtgtc atttggttgc gcctatagat agttacgccc ttattttaat agattcttgc
     4141 tagatgaatt gccaaataga tgatttcatt ttcttgaatg gcaagttgaa attgctgttc
     4201 gatgatatct ttaatcttta gtgctgtaca atacgctttg ctatgtttaa atgtttcagt
     4261 aaaaaaacca atattttcta ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa
     4321 tttgatatga accattaagc gatgataagt cagtgattct ttatcataaa cgtttgaata
     4381 ggatttttct agatgggaca aaatcgtggt cgtcatttct actattttta agctctttaa
     4441 actattaggc tctcctagct cagcattaat gatatgcata gtgatgaatc ctacttcatt
     4501 tgaccctaac tcggtttgat atcgatgatt gattcgctcg accacttcta ctgctatatc
     4561 atattcattt ggataaagga atctcatttc tggcaacata attgctgtga catctctatc
     4621 ttctcttttt cgttgaatag caaaataaat atgatctgtc aatgtaatat agatatttga
     4681 atctaatgga gaggagatcc tagctgttat cagttcaatc acttcttccg caaaatccat
     4741 aaattcgatt ggaatctgcg agactatctc actgaatcgt ttgatctttt ctttagaatc
     4801 taatacaaat aatttgtcag catcctcaac atcaaatggc ttactcactt tagcagaaaa
     4861 gccaatccct ttccccatcg caattttttc tattccatcg tcatcgatca atacagcatt
     4921 atgattcagc tttttcatca catacaccgt taattcacct cctctatatg ataaaacaaa
     4981 acaacttgca gactccttca tacatatttt tgtctccaaa caatttttaa agacaaaaaa
     5041 cgacctaaga aagaaccctt tcttaggtcg tgcctaattg aatagtagca ccctacataa
     5101 tttacaaaaa tagtttaaca cataataaaa gcgttatcaa tatctttttt actcgtttta
     5161 tgataatact tgtatcgaat tgatttggag gtaattacat aaaaacaaaa aaaatatagc
     5221 aactctctat aagaaagttg ctatattgat aattataccg gcggccgggg tcgaaccggc
     5281 acgtcctcaa cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc
     5341 aaatttaaaa ataataactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt
     5401 cccttgcctt accgcttggc tataccccaa gaataaaggg cgaatgatgg gaatcgaacc
     5461 cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatcataaa
     5521 agatgttatt taatttctca ataaaaaact aagatgaaga catcttagtc aataccggcg
     5581 gccggggtcg aaccggcacg ccctcaacgg
//
LOCUS       DAZUMB010000142         5556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000142 DAZUMB010000000
VERSION     DAZUMB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.146"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..192)
                     /locus_tag="ACG80L_002464"
     CDS             complement(<1..192)
                     /locus_tag="ACG80L_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024663.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNN"
     gene            354..1463
                     /gene="pdhA"
                     /locus_tag="ACG80L_002465"
     CDS             354..1463
                     /gene="pdhA"
                     /locus_tag="ACG80L_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS5024664.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1466..2443
                     /locus_tag="ACG80L_002466"
     CDS             1466..2443
                     /locus_tag="ACG80L_002466"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS5024665.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2468..4111
                     /locus_tag="ACG80L_002467"
     CDS             2468..4111
                     /locus_tag="ACG80L_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS5024666.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4119..5525
                     /gene="lpdA"
                     /locus_tag="ACG80L_002468"
     CDS             4119..5525
                     /gene="lpdA"
                     /locus_tag="ACG80L_002468"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS5024667.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT         1856 a         1040 c         1263 g         1397 t
ORIGIN      
        1 attatttgta cgtccgttca cgatgtgtaa ataaaataaa tgtttgcaac aaaactgtat
       61 aacaccaacg tttttaaata gaaacagatg gttgtaagtt tgattagaaa atagaaaaaa
      121 tgcagatgaa aaataaaacg ctttccttat tgtgatttta ttcactaatg ctgttaatat
      181 gcggattggc ataatttcct ttcatcgtga aaatttgtgc ttttagcttg tatttctcat
      241 aaatccatga taatttatta atgaagacgg ttaaatagtg atacagtttt attcactgtt
      301 tcaatcgcag aatgaagaga ttttcttata gatgagagag gaatgtgtga caaatggcaa
      361 acaaaaaagc accgatcgac ttccaagcac tgctagatga agtcaatgca gatttcccag
      421 tatatcaagc acttgatcaa gatggaaacg tagttaacaa agatttagta cctgatttat
      481 ccgatgatga actcgttgaa ttaatgactc atatggtatg gtctcgtgta ttagaccaac
      541 gttcaacagc gctgaaccgc caaggacgcc taggcttttt tgcaccaact gctggacaag
      601 aggcaagtca gctggctagt gcttacgcat ttgataaaga agatgttctt cttccaggtt
      661 atcgggatgt acctcaacta gtaaaacatg gtttaccttt atctcaagct tttttatggt
      721 ctcgaggaca tgctgcaggt aattcttatc cggaagaatt gaaagctcta ccaccacaaa
      781 ttatcatcgg tgcacaatat gtacaagcag ccggtgttgc attaggtttg aagaaacgaa
      841 acaagaaaaa tgtcgtcttc acttatactg gtgatggcgg ttcatcacaa ggtgattttt
      901 acgaagcaat caatttcgct ggcgcttacc atgcaaatgc cgtattttat atccaaaaca
      961 acgggtatgc gatctctaca cctcgtgaaa aacaaactgc agcaaaaaca cttgcacaaa
     1021 aagctgtcgc agctgggatt cctggtattc aagtcgatgg aatggatccg cttgcagtat
     1081 atactgtatc taaaatggca cgggaatggg cgatcagtgg aaatggacca gtcctaatcg
     1141 aaacactgac ttatcgttat ggaccacata cactatctgg tgacgatcca acccgctacc
     1201 gtacaaaaga cacagatgac gattggcaga aaaaagatcc attgatccgc ttccgtaaat
     1261 atttgacgga aaaaggactt tggtctgaag aaaaagaaga acaagtcatc gaagcaacta
     1321 aagaagaaat caaagcagct attgctgaag ccgacaaagt accaaaacaa aaggtttcag
     1381 atttcttgaa aaacatgttc gaagtatctc cacaatcaat caaagaacaa attgcaattt
     1441 atgaagcgaa ggagtcgaaa taatcatggc acaaaaaaca atgatccaag caatcacaga
     1501 tgccttagca cttgaactag aaaatgacga gaatgtcgtt gtttttggtg aagatgttgg
     1561 gaaaaacgga ggagttttcc gggcaactga aggattgcag gaaaagttcg gagaagaccg
     1621 agtattcgat actcctttag ctgaatctgg aatcgcagga ttgagtttcg gtcttgcgtt
     1681 agaaggattt cgtccagttc ctgaaatcca attctttggt tttattttcg aagcaatgga
     1741 cgaagtagta gctcaaatgg ctcgtacaag atatcgaatg agcggcacta gaaatctacc
     1801 catcacaatt cgttcgccat ttggtggcgg tgttcataca ccagaattgc attcggataa
     1861 tttagaaggt ttgatcgctc agtctcctgg tattcgagta gtgattcctt ccaaccctta
     1921 tgatgcaaaa ggactattga tttcttctat tagaagtaac gatccggttg ttttcctaga
     1981 acacatgaaa ctatatcgct cattccgaga agaagtgccg gatgaagctt acgaagttcc
     2041 tttggataaa gcagcagtta ctcgtgaggg aacagatgta tctattatca catacggagc
     2101 aatggtgcgt gaagcaatca aagcagcaga caatcttgca aaagaaaata tatctgttga
     2161 aatcattgat cttcgtacag ttgcgccatt agatgtagaa acgatcattc aatccgtgga
     2221 aaaaactggt cgcgtagttg ttgtccaaga agcacaacga caagcgggcg ttgccgcgca
     2281 agtagtttct gagatctcag aacgcgctat cctttcatta gaagccccaa tcggacgagt
     2341 ttctgcacct gatacagtct tcccatttgg acaagcagaa aatgtctggt tgccaaatgc
     2401 aaaagatatc gaagacaaag taaaagaaat cgcggaattt taagaaaaga agggacgaaa
     2461 caaagtcatg gcttatcaat ttaaattgcc ggatatcggt gaaggaatcg cagaaggtga
     2521 aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga
     2581 agttcaaaat gacaaatcag tagaagaaat cccgtcacct gttacaggaa cggttaaaaa
     2641 tgtgattgtt ccagaaggta ccgtagcaaa tgttggggat gtattagtag aaatcgatgc
     2701 accaggacac gaagacaatg aaggggacag tggtgtagcc gcagaatccc aaactccagc
     2761 taaaccggct gctgaaccca ctgttgatac agaatcagct ggatcgtcaa gcgaaggagt
     2821 cttccaattt aagttaccgg atatcggtga aggaatcgca gaaggtgaaa tcgtcaaatg
     2881 gttcgtcaaa ccaggagata cgatcaatga agatgataca ttactagaag ttcaaaatga
     2941 caaatcagta gaagaaattc cgtcacctgt cacagggaca gttaaaaacg tgattgttcc
     3001 agaaggcact gtagcaaatg ttggggatgt attagtagaa atcgatgctc ctggacacaa
     3061 tagtgcaccg tcaactagtg cgccatcagc tgaagcacca aaagaaaaag tggaaacatc
     3121 aggttcagca agtgtcgttg aagctgccga tccaaataaa cgtgtattgg ctatgccatc
     3181 tgttcgtcaa tttgcccgtg aaaaagatgt agatatctca caagtaactg caactggaaa
     3241 aggtggcaga gtaacaaaag aggacatcga aaacttcttg gcaggtggtc caagcagtgc
     3301 accagcgaaa tcagaagcgc cagaagcggc agcaccgaaa gaagcagctc ctgcagcaga
     3361 aagcaaacca gctgcaccag caaaaccatt caagtccaac ttaggagact tggaagaacg
     3421 tgtagcaatg acgccaacac gtaaagctat cgctaaagca atggtcaaca gtaagcatac
     3481 agctccacac gtgacattgc atgatgaagt agaagtgtct aaactatggg ataatcggaa
     3541 acgatttaaa gaagttgctg ctgcaaatgg tacgaaactt actttcttgc catatgtcgt
     3601 gaaagcttta acagcgactg ttaagaaata cccagtattg aatgcatcta tcgacgatgc
     3661 aaaccaagaa atcgtttata aacattacta caatatcggt attgcaactg atacggatca
     3721 cggactatat gtaccaaatg tgaaagatgc agaccgcaaa ggaatgtttg ctattgcaga
     3781 cgaaatcaat gagaaagcaa aattagctca tgacggcaaa ctatctgcag aagatatgcg
     3841 caatggaaca attacgatca gcaatatcgg atctgtaggt ggcggttggt ttacaccagt
     3901 catcaactat ccagaagtag ctattttagg tgtaggaacg attgcacaac aaccaatcgt
     3961 aaatgctgaa ggcgaaatcg ttgtcggtcg tgtgatgaaa ctgtcattaa gtttcgatca
     4021 ccgtatcgta gatggagcaa ctgcgcaaca agcaatgaat aatatcaaac gtcttttagc
     4081 tgatccagag ctattaatga tggaaggatg ataaacaaat ggtagttgga gattttgctg
     4141 tagaattaga tacagttgta attggtgcgg gacctggtgg ctatgtagct gccatccgcg
     4201 ctgctgaaat gggacaaaaa gtagcaatca ttgaaagaga gtatattggc ggtgtttgtt
     4261 tgaatgtcgg ctgtattcct tcaaaagcac tgatagctgc aggacaccat tatcaagagt
     4321 cactagattc tgaaatgttt ggagtaacta gtgaaaacgt caaactagat tttgcaaaaa
     4381 cacaagaatg gaaagaaaac aaagttgtcc atacgttgac ttctggtgtt ggtttcctat
     4441 taaaaaaaca taaggttgaa acaatcgaag gagaagcgtt cttcgtagat gatcacacac
     4501 tacgcgtcat tcatccagat tcagctcaaa cttattcgtt caataacgca atcatcgcaa
     4561 caggttcccg tccaatcgag attccaggat ttaaattcgg aggacgtgtg ttagattcta
     4621 ctggcggatt agcattgaaa gaagtaccta agaaatttgt gatcatcggt ggcggtgtga
     4681 ttggtgccga attgggcgga gcgtacgcaa acttaggagc agaagtaacg attcttgaag
     4741 gctcaccaca aattttacca acttacgaaa aagatctagt aaaattagta gaagatgatt
     4801 tcaagaaaaa aggcgtaaca gtcgtaacga atgcaatggc gaaagaagca gtcgataacg
     4861 gtgacagcgt gacagtgaaa tatgcagtgg acgggaaaga agaatccgtt actgctgatt
     4921 atgtgatggt cactgtaggt cgtcgtccga atacagatga tatgggctta gaacaagcgg
     4981 gtgtcgaagt aggagaacga ggattgatca cggtggataa acaaggacgt acgaacgtac
     5041 ctaacatcta tgccatcggt gatatcgttc caggggcggc attagcacac aaagccagtt
     5101 atgaagcaaa aatcgctgct gaagcaatct ctggcaaaaa agtagctgta gattataaag
     5161 caatgccggc agtagccttc actgatcctg aattggcttc tgtaggtatg acgatcaaag
     5221 aagcaaaaga tgctggtatc gaagcaaaag catataaatt cccattttca gggaacggtc
     5281 gggcattatc gcttggtaaa acagaaggat tcatccgtct agttacgacg atcgaagata
     5341 acgtgcttat tggcgcgcaa atcggtggag tcggcgcaag tgatatggtc tctgaattag
     5401 ctttagcaat cgaatcagga atgaatgctg aagatatcgc cttgacgatt catcctcatc
     5461 catctcttgg cgaaatcgtc atggatgctt cggaattagc attaggttta ccaatccata
     5521 tttgatttga ataatacagg cgaaacgcaa ctgaaa
//
LOCUS       DAZUMB010000143         5555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000143 DAZUMB010000000
VERSION     DAZUMB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.147"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG80L_002469"
     CDS             complement(<1..281)
                     /locus_tag="ACG80L_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5024668.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACG80L_002470"
     CDS             453..1541
                     /locus_tag="ACG80L_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5024669.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACG80L_002471"
     CDS             1669..3045
                     /locus_tag="ACG80L_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5024670.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACG80L_002472"
     CDS             3446..5026
                     /locus_tag="ACG80L_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS5024671.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACG80L_002473"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACG80L_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
BASE COUNT         1870 a          910 c         1134 g         1641 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagtt
//
LOCUS       DAZUMB010000144         5532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000144 DAZUMB010000000
VERSION     DAZUMB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.148"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..985)
                     /locus_tag="ACG80L_002474"
     CDS             complement(<1..985)
                     /locus_tag="ACG80L_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFS5024672.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACG80L_002475"
     CDS             1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACG80L_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS5024673.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="ACG80L_002476"
     CDS             1810..2964
                     /locus_tag="ACG80L_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS5024674.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="ACG80L_002477"
     CDS             3179..4147
                     /locus_tag="ACG80L_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS5024675.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="ACG80L_002478"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="ACG80L_002478"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS5024676.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5532
                     /locus_tag="ACG80L_002479"
     CDS             5177..>5532
                     /locus_tag="ACG80L_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFS5024677.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1669 a         1078 c         1252 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc gg
//
LOCUS       DAZUMB010000145         5488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000145 DAZUMB010000000
VERSION     DAZUMB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.149"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      50..216
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            364..921
                     /locus_tag="ACG80L_002480"
                     /pseudo
     CDS             364..921
                     /locus_tag="ACG80L_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            979..3018
                     /locus_tag="ACG80L_002481"
     CDS             979..3018
                     /locus_tag="ACG80L_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5024678.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFNNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3127..4278)
                     /locus_tag="ACG80L_002482"
     CDS             complement(3127..4278)
                     /locus_tag="ACG80L_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5024679.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4432..4962)
                     /locus_tag="ACG80L_002483"
     CDS             complement(4432..4962)
                     /locus_tag="ACG80L_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5024680.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5061..5411)
                     /locus_tag="ACG80L_002484"
     CDS             complement(5061..5411)
                     /locus_tag="ACG80L_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS5024681.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         1983 a         1011 c         1037 g         1457 t
ORIGIN      
        1 tgcctaatca ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg
       61 ttaggtgagg ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg
      121 ggtaaaacag aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg
      181 ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa
      241 ggtttctgac cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc
      301 ctcacctgac aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt
      361 ggaggtcgct cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa
      421 ctgatctcta tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata
      481 aatgcagctt actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc
      541 aaatttgaag ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat
      601 gaactgtttc ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta
      661 tatataaaca aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt
      721 cgggaagaca aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct
      781 atctctcagc agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa
      841 ttacttggta gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat
      901 ttcctcaaag acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag
      961 caagagaagg aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct
     1021 tatattgccg gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag
     1081 cactatcaag aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa
     1141 gctttgaaca agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat
     1201 aaaagtgcat tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct
     1261 gatgtcaaag gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac
     1321 agtttttcat ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat
     1381 aaaggaacat tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt
     1441 tttccagaaa tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg
     1501 aatattatag atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga
     1561 gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct
     1621 tcctccttcg acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa
     1681 cccgattact ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga
     1741 gctaccattc aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg
     1801 attggttacg tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt
     1861 aatggaaaaa tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca
     1921 acaggtggga aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa
     1981 catgaagttc aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaata
     2041 gcttttgaca gtctaggagg aaaagctgca tcaactgttg cgacaacgcc aaaaaccggt
     2101 gatcttcttg cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc
     2161 tcacaagaag attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt
     2221 gcgacaggtt atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac
     2281 aacggcacta tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac
     2341 agttcttggg gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta
     2401 aaaactgctt tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt
     2461 gagaaaaaat ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca
     2521 attagtatga atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta
     2581 gctgatactg gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg
     2641 tattctgttt ttaacaacaa tggcacactt gtctatccta aattgattgc agataaagag
     2701 acaaaagata agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg
     2761 agagaagttg tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg
     2821 ttggcagcga aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag
     2881 aacagtttct tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg
     2941 gaaaataaag aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc
     3001 aaccaaaatt atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc
     3061 gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg
     3121 atttactcac acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag
     3181 tgtacttaat aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact
     3241 ttgctgtcca atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc
     3301 gaccatcaat gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag
     3361 caatttggtc aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa
     3421 gtcagaaaaa tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata
     3481 tcctaaagcc tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat
     3541 cccagcaaaa aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat
     3601 tcctaaacaa ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc
     3661 aaatgaaagt aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat
     3721 tagctgttca atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa
     3781 agataggctg aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa
     3841 aattgttgat ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat
     3901 actgacagaa gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg
     3961 gaatgctttg cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac
     4021 gaacatacca ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa
     4081 aaacattaat aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac
     4141 ccaattcaat ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg
     4201 gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa
     4261 aataccaata aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat
     4321 aacatacatt attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga
     4381 tttttttgga ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat
     4441 agtcattttt cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat
     4501 aaagccgaat atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa
     4561 tccgccaatc aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat
     4621 gctgaagaac aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag
     4681 gatagaaacg attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag
     4741 accactgaac agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa
     4801 aagattcaac aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa
     4861 aacagcagaa gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa
     4921 taaggcgtat ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg
     4981 acttatttta acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga
     5041 gaagaatatc tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct
     5101 agctcatcga tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct
     5161 tctactcgta caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg
     5221 gcttccgcaa aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt
     5281 tgggaataac ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt
     5341 ctctccgctt ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga
     5401 ttttgcgcca taatcagtca cctttctttt tttcaaatta tttactactt atagtatata
     5461 agaaaaagag aagaaaaagc gattattc
//
LOCUS       DAZUMB010000146         5474 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000146 DAZUMB010000000
VERSION     DAZUMB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.150"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..291)
                     /locus_tag="ACG80L_002485"
     CDS             complement(<1..291)
                     /locus_tag="ACG80L_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024682.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYI"
     gene            complement(589..1563)
                     /gene="sgrA"
                     /locus_tag="ACG80L_002486"
     CDS             complement(589..1563)
                     /gene="sgrA"
                     /locus_tag="ACG80L_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS5024683.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1759..2598)
                     /locus_tag="ACG80L_002487"
     CDS             complement(1759..2598)
                     /locus_tag="ACG80L_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5024684.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2783..3670
                     /locus_tag="ACG80L_002488"
     CDS             2783..3670
                     /locus_tag="ACG80L_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS5024685.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4254..4949)
                     /locus_tag="ACG80L_002489"
     CDS             complement(4254..4949)
                     /locus_tag="ACG80L_002489"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS5024686.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4933..5331)
                     /locus_tag="ACG80L_002490"
     CDS             complement(4933..5331)
                     /locus_tag="ACG80L_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5024687.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1744 a         1098 c          802 g         1830 t
ORIGIN      
        1 aatataacca atcgttccct ctaaaagtat agaataaact ttactcagta aaaactgaga
       61 agaaaactcg acttttatca tcttgttttc tgaaagaaaa gataatttat attctggata
      121 cctttcgcaa aacgattctg cttgttgaat caccgaattc atcttagcca gggtgtagtt
      181 tagatctaaa gcgatatcct tcaccacata taatttctcg tctctttcat aacaatagtt
      241 caaaaattga tatacttgcc tttcattgat atcaataaat tcttcaaaca aagtacatac
      301 tcctttttat ctattattta gacttattcc gacagctaca tgaataacgt acaaatagat
      361 tcgttactga tttttcagag atactaccat gcgcagtgtg cattgtcaat tattcttgct
      421 cttattttcc atttgtgttc ctgttatttc ctgtttctca aatatgttag taaacaaaaa
      481 tagatctggc aaagctacaa cttcgccagg caatacagca ctctcgaatg aaatattcac
      541 tcaaaaatct atcgttttat tcaattttag atctgttgct tttattcttt atttcactcg
      601 atggaagaga gaacgaaaat agcttgctcc tacaatcaaa atgagtaaca cacccatcca
      661 tgtaattagc ttgttcaagg ttctactacc agtttctggt aaatcgcttt ttctattatc
      721 tgtttctttc gctttttctt gatgatcaat tccacgagaa acaaaatcag gctgttttag
      781 cacacttttt aggttgctcc ctgcatttct cttttgttcc ttagttggta tgataggtac
      841 caaatcaagt tcattattta cttttcctgt gctgctttcg ctgctcgtgc ttcctgtgct
      901 actctcacta ctcatgcttc ctgtgctact ttcactactc gtactctccg tactgctttc
      961 gctgctcgtg cttcctgtgc tactttcact actcgtactc tccgtactgc tttcgctgct
     1021 cgtgttttct gtgctacttt cactgctcgt actctctgta ctgctttcac cgctcgtgtt
     1081 ttctgtactg ctttcactac ttgtgctatc cgtactgctt ggcggtccaa caactgtcca
     1141 actgcttggt ttcaaattct ccgtgatcgt catctgtcca tcataaaaaa caatcaaatc
     1201 aactttatct ttactcttga tctcatcaat cgatagttct gcagaaatca ttattgtata
     1261 ctcaatacca gcggtccatt gtacgtcttt taaataaact tccccaataa ctttttctga
     1321 acttccatta ccatcccaac catatgttag aacagcatgt acatcactag cattcacgct
     1381 tttgtttggt ttgactggaa tagtaatgac ttgttgtgtc atgtcataaa caatccgatc
     1441 agttttcaca gtaaatgaaa gattctcatt tgcccgttca tttgcagaag tactgattcc
     1501 aatcattgct gcaattatac tcagcccaag aataacaaat aatacggtcg ctgttttctt
     1561 catttctatc actctccata taattacctt ctatttgata aaggaattat ataaaatgaa
     1621 aatgatgaga acacgacagt cagttgtgaa aatctaacaa aagatgtcgt gtattcgctt
     1681 gaatttgttt actttttcgt ttaatcaatg acagacttaa aaaagcaaga ccgatatcag
     1741 ccttgctttc ttcatctttt attgataaac agcagtgtat ttcttcatcc gctttgtttt
     1801 atatgcatag agtaacaaac caacaatcaa gactaacaag aataatgtag ttgatataga
     1861 tgcttcagcc ccaaagtttc ccccagtaat tattgtattt tgcgattgga tcagagtgga
     1921 gaaaaaacct tgttcttgcg ttccgctgac tggtataccc aaaatagctc cttgtgtcca
     1981 attccaagta atatgagcac caatagccgt aaataaactt cctgtactgt agataagtgc
     2041 gctgaaaaag caacctgcta aaaagatatt gaaaactgcc agaaatgtca tgttaggatt
     2101 caatccgtgc aaaagagcaa ataatgcagc agatccaatc attgctatta acggaccaaa
     2161 tctaagcatc tgatgctgaa aaaagccacg gaataaaagc tcttctgatg cccccactgc
     2221 aataaataaa ataaaactcg ttatcagcgg cacaatgttt gcgtttcctg aatctaaccg
     2281 atacgccccc gtcaaatata atggactagt aataagcaga aacactccaa ttccaacaac
     2341 gagacctccc gctaaatacc cccagtcttt tcctcgaaca gaaacaccat aatcttctat
     2401 tctttttcct tcgaccttac tagcaagaaa taaagctagt aaaaaaacag ctaaatgtcc
     2461 gaccgtaccg gcaattgctc caatccattc actatttcca ataaacatat ccgcaagtgc
     2521 attattgaca agaattccta aaacaaaacc aactaagact attaaaacag atccaatcaa
     2581 ccgaataatc ctgatcatct ttcatccctc ctttatatta agtttaacaa cccttttctt
     2641 tcttgtctcg tgaaaactat gtaaatgttc tccatcatag agagaataga tttttacgaa
     2701 ctaagaatat ctacagcaac cttacgaaaa tgaaagactg tctccctaat tcttctgtta
     2761 gactgttttt aataaggaaa ggatgggaaa ttttttgaaa aaacaactac taattatgtg
     2821 cctttcagca tgtgcaattt tactattctc aggttgtagc aagtccaatg aaacgattgc
     2881 aacgtttaaa ggtggaacac ttacagttca ggatttttat gataacccaa aagttaaatc
     2941 aaataatcaa acactattaa aaaaaatgat tgtcttcaaa gcatttgatg atgtatacgg
     3001 aaaagagatt tcttcaaagg aagtaacaaa agaatataat gaacaaatca aaaaactcgg
     3061 tccaaactac aaggaacaat taaaagcagt tggccaaaca gaagagacat ataagaaact
     3121 ctttaaacag atgctggctt tccaatacgg tttgaaagcc aatgtaaaat taacagataa
     3181 agatctagac actgcttgga aagaattcta tccagaagtt agtacacaga tcattctatt
     3241 ttcgactaaa gaagaagcag ataaagcaaa aaaagaagca aatgaagggg aaaatttctc
     3301 caagcttgtt caagcttatg gaaaaaataa aacattaaag gaaactgatg gaaaaatgaa
     3361 ttttgattca accaatccag agattcctac tgaagtaaaa aaagcagcat ttaaactaaa
     3421 aaacggggaa gtttctgata tcattcctgt aacagatcca acgacttatc agcaaagcta
     3481 ttatctagta aaaatggtaa aaaaacagga taaaggttct aataaagaca aatacaaatc
     3541 tgagctagaa aaaattgcta ccgaagcaaa aactcttgat acggaattca tggataaaac
     3601 aatccgtaaa gtgatgaaaa aagacaatgt aactattaaa gatccttatg taaaaaatat
     3661 attcaaataa acaaatttga cccaattggg gatgtggcaa aatttgagaa gtgatttttg
     3721 gaaattcctt cttatttttg aggcaaacca cttttgccac aacttcatcg gggccttttt
     3781 ttatttgtac caaaacaaat cactggattt gttacaatca tgaggatata atttaaaaaa
     3841 gttttttatt tctatcacat gaatcaatgc gccattaagt agattctttc atgcttccct
     3901 attttttcac ttgccgtatc gcttcaaaaa caactatcat aaactcaaac tcgactgaaa
     3961 agttatttta aaaaagacta aaaatatccc ttctggactt gttcctctta ttctgttata
     4021 ctagagaaac acgaaagata tttttatgcg acacttttca tttgttttta tgaaatttat
     4081 tcaaaactgg cgtaacttca gagttacaag gatgtattag aggttaggga atacatcgtt
     4141 taaatgagaa ataagtaata gtcacccaaa ctttaaatta atgaatattg aatcgtcaga
     4201 tagcctcctc gagttttcga gaaggctatc gtttatttta tggagaaatt gattcagaag
     4261 taatcagcga ccttttttag ttcaccaatc gtttctgctt ggaaagtttg cacccaatct
     4321 tcttggaagc ccctcactgc tttatcaatc gagggcatct ctaatacttg atctagtagc
     4381 tctttaccta acgttatcgc atgagctcca gatcgaatgg ctgtagtgac ttgttcacaa
     4441 tttttgaaac tagctgctaa tatttttgtt ggtctttgcg tacgttcgat ctcagtccgc
     4501 aacgaatcaa tcactttcat agcatcaatg ccttgatttt gcatcctgtt ataataaggt
     4561 gcaatataat ctgctccatt attaatagct aaataccctt gtaattctgt gtaaatagct
     4621 gtaccggtaa tcaaataacc ttcttttttc aactttttga tcgcttgaag gcctgcttga
     4681 ttcaccggca ccttgataaa cgtatctttt cccagttctt ctatgataac tcgagcatcc
     4741 tctatcattt cttcttccgt tcttcctatt gtctgaatat ggataggaat ctgtccgatt
     4801 atttttttga tttctttcat atgcgagaag aagtttgacg ctccttcttt tttcagaatt
     4861 gtaggatttg ttgttacgcc tgttaaattc aaaaactgtt gacaagaata aatacttttt
     4921 gtatttgctg tatcaagcat gatttccata tagctttccc ccattctgat cctcttgatc
     4981 gattttaatg attgtcccct ttcttaaatg cggcatttcc ttatcttcta catacattgt
     5041 tcctggaagt tccggctcat gagatccatc aaattttatc gtgatatgtc ctaattcatt
     5101 caaattagtt tggacaacat ctcctactgc tgtgattaca tacatttgtt tatcaaaaca
     5161 gagcttcata tcacaagtta tttcttttct tacctgattc aattcaatct tataacaata
     5221 atctgttaat gtttccggtg cttcatctcc aaataaaatc attatttttt ctttcctgaa
     5281 tacttctgct tctataccaa catctgttac aaccgtttca aagatcgtca tctctgttcc
     5341 tcctcgtaaa atatcatttg tctaatagtt tttattcaaa atgtttctag attataaaat
     5401 ttattgaata acacattcct aacggaattt gtttcgatat tgtgtgattt ctcagagtac
     5461 aatggatgta tgga
//
LOCUS       DAZUMB010000147         5472 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000147 DAZUMB010000000
VERSION     DAZUMB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.151"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACG80L_002491"
     CDS             complement(<1..450)
                     /locus_tag="ACG80L_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024688.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRA"
     gene            complement(492..713)
                     /locus_tag="ACG80L_002492"
     CDS             complement(492..713)
                     /locus_tag="ACG80L_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024689.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(710..1000)
                     /locus_tag="ACG80L_002493"
     CDS             complement(710..1000)
                     /locus_tag="ACG80L_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024690.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(997..2181)
                     /gene="mobT"
                     /locus_tag="ACG80L_002494"
     CDS             complement(997..2181)
                     /gene="mobT"
                     /locus_tag="ACG80L_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5024691.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(2363..3766)
                     /locus_tag="ACG80L_002495"
     CDS             complement(2363..3766)
                     /locus_tag="ACG80L_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5024692.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3788..4561)
                     /locus_tag="ACG80L_002496"
     CDS             complement(3788..4561)
                     /locus_tag="ACG80L_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024693.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4571..4948)
                     /locus_tag="ACG80L_002497"
     CDS             complement(4571..4948)
                     /locus_tag="ACG80L_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5024694.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4969..5283)
                     /locus_tag="ACG80L_002498"
     CDS             complement(4969..5283)
                     /locus_tag="ACG80L_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5024695.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
BASE COUNT         1609 a         1092 c          929 g         1842 t
ORIGIN      
        1 ggcacgttga tattcaccat tactcataaa agactcagcg tcttcaatag cttgtgaaac
       61 cacatcacta attttcaact gactaaagtt aattacttcg taattctctg gaagtggtac
      121 taaaatagta tccactaata cattatcacc ttgtttatca tatatggagc ttgctgtctg
      181 ggtaatcaaa tctttatgac tttcaaaaaa gtctaaatca tcaaaaggaa ctcttatttt
      241 aagatatgtg caaagcgtat tccagccctt tttatatcct ttataataag taaaatcgtc
      301 agtctcatct aaagttacct ctgatatttt cattttagaa acaatctcgt tacctccttc
      361 ataataattt gaagagctct ttctttctaa tgttttaatt aaaagagatt taaattgatc
      421 atttgtaaac atattcataa atcctttcat aacaaaatat atacttccta ttttatcatg
      481 aaaaatatct gttaagcacc aatgattcgg ttaaataagt ttaataacac atctttcacg
      541 ccagctgcat tgaacacaag tccgacggcg attagagcaa taatcaagaa gccaatgagt
      601 tttgaaaatt ctcgtttaaa acctaaaaac agtccaatga caacaatcgc taacaatacc
      661 aatgactgtg cattatccaa gaaccagttg tacaaattct gcccaaaatt catgtgattg
      721 tcctcctttc cttaagatat ttttctcgat aatagatata gctttttaca cgccttaata
      781 attgaaaagg ggttaaatgc tctccataga cggttactcg attggtataa ttattataaa
      841 atctcacctt gtaaaaatca taggtataga tggtatcacc agcttgccgt tttccctcaa
      901 taatgagaga cgtatatttc cctgtatttt gtatatacgc ttgaaactca atgccacact
      961 gaaaccctaa cttctttaat tcttttgtta aaaggctcat gtaatcaaat cctccatggc
     1021 tagatgttgt tgttcaatta atttctcatg tttttctgtc agtttggctt cctgaatcat
     1081 ggtggagata atggttgtac cgttgagaac atctaaaatg cttgcgattt ttaatgtagg
     1141 ggcgacttga tgatgtaacc agttcaatgt tcgttcaaaa gaataaggct ctggctgtgt
     1201 ggttaggcgt aatgctccac gatttttacc gataaaccat tcccaacgtt cattagtttt
     1261 ccaatcactc cgacgtttag tgtcgtcttt atctgcaaag cggatatagc gattgataat
     1321 ttcaaaggct gttttttccg cctgttgatg tgccaataaa tctttcatgg cttgggtggc
     1381 tcggtcattt tttaaccgaa tttcaaaccg attctttact tctgcgtctt ctaagggaac
     1441 atcatttttc acgtattgct cataatcttt ctcatatata cagaaataga cttcactttt
     1501 taatgagcca atataaagtg tgtttcccat gtatggtttt tcatcacggt gaaccaattc
     1561 accactgcga taatttttaa agcttcgaaa gacggaaatg cattcttcct gtttacattt
     1621 ctttgccaaa tctggaatat tcaaaatccc tgttttatcg ttgatagcta aatccaagcg
     1681 tttgaaaata cctttatgtt ccatgcaatc ttgaaagaaa tcataccagc tccgatgttg
     1741 agccaataga aagttttcaa attgccgaca gccttttcct ttcagttcta ataaaacacc
     1801 tttacttaca tcatgagaga ctaggacaac aatatcaccg aaacgatagt gttcgggata
     1861 agaataatag ccaaagtctt cgtgtagcat ataatccata ttcaaacgta aaacctcacc
     1921 gataatttga gccacatcat tggtagggaa tcgaatccgt acatagtcga ataacatctc
     1981 caatgggtta tcgggattga agcgttccaa agcgttcagt aaatcttctt tcacctgttg
     2041 agtagggact gccttgcctg tttcaatatc cgataggtaa ggtctggtaa tatgagaagc
     2101 aaccgccagt ttattttgag aaacgccata agccagtcgc ttttctttca attccttaat
     2161 ccaaattttt tcgttttcca ttcgcaaacc tccaatctaa aaaagatgta aactatgacc
     2221 cttgagttta cacctttcat attttccgca atcctttgta ccatcaacat tggttgatga
     2281 gaattgcctg ttttcagttg tatttgtgcc cccctgttag atagaggggg ctaaaggatt
     2341 ggcgtggcta acgccacacc aatcaaagca agtctgaacc tgtacctttc acttcgtgcg
     2401 gtacaggctc agattgcttt ttttgaatga cttttcctat ttctcctaag aaatcgtacc
     2461 tttttggtac aaggggtgta taaaactcac taatcacact ttttcctgta tccacgtaac
     2521 ctcttccctt gatgggtttt aagaaaaatt gtttttgtgt atcgcttccg aacatcatac
     2581 caaagcccat ttcagacata cgacccaaag ccacacggaa gttaaagttg tcccgaatcc
     2641 catcactaaa atattttgcg tctggacgtt gacaggcaag aattaaaaag aagcctgctt
     2701 gtcgtcctaa cattacaatt tgtttgagtt tatgaataac ggtggtactt tcacgaccta
     2761 gcatatccat cattgccaca tattcatcaa aaataagaaa attggcagac aaaccaagat
     2821 aagcataatt ttctcctgtt ttatagttag ggtgttgttt catggtttca ctgcgatcaa
     2881 tcatatcttc gtaaaattga ttgatacatg ccaacatatc ttctttacgg taatagacat
     2941 tattcatgac cgcccctaag tctgccaaat cagcattttt agggtcaaga atataaagct
     3001 ttgcatttgt atgcaggaga gattcaatca atgtgagaat aaaataggat tttccactac
     3061 cagtgccccc agcaattaac atgtgaggga gagaatcgaa ctcccaatag accgattcca
     3121 tcaatttgac acgtccatct tgagctacca cgtcttcaat gccaattcga ttgccaatag
     3181 tatcataaag taggacatac tctacataag aatctttcaa cgttttttcc actaattcac
     3241 aatacaatcc tgtttctagt ttgttttcta ggtgcaaaag ctgatcttga tatttcccta
     3301 aggtgatttc tacatgaata tggattagtc catctttcat tcgataatat attttaggaa
     3361 aatggcggat tcgttgtttg gttttcttgc tgggtaaatc tttaaagaat ccctctgatt
     3421 gagtttcttc ggcttcatac caatgatttt ctaaaatcat acgagctaac ttttgacggt
     3481 gctctaactg ttttatagaa tctatacgat agtgataata aagccagctg ataagtccgc
     3541 atacaaaaag agcagtgaga atactgctcc aaaagtatgg cgtatgaagt tgatagaaaa
     3601 atgtatctaa ctgaactgct tgccagttca ccgattgtaa cgctctccaa tgaaaaggga
     3661 gtaggacgac tagaaaaact accagcaatg aaattagtac aaaatgatag accaagtgct
     3721 tatcgttggc tttgattcgc tctcctctgt catgaagcaa tggcataaaa atcctccttt
     3781 ccacctatca aaataattgt gtattgtaga tacgagcctg tttatcaaaa gttggttgag
     3841 aaatggcttc atatttctga taagcgattc gcttttcaaa gcgtaaattt ttttctttcg
     3901 gataataatg acgtcgatag agagtgtgtc ctaacagttt tttcgcttct tttgtcaacg
     3961 gatagagata gcgaaacatg cgtccattga ttttctcaat ccctttgtat tcgcaaaatg
     4021 cctgggaaag ccaatgcttt ttctctacct gttcaaagcg agcattttcc tctaataaaa
     4081 ggcgagcact tcgagggtgt atcttttccc atgactgctt atcacgatag acacttgtct
     4141 tgaaataccc gcagtaaaag aagttggacg cttgatagac atagccacat tttccctcaa
     4201 ttccatctgc cagtgtatat aaaaaatgac agtccgtatg ttctttcaac cacttaatta
     4261 gagctgaaag agcttgactg ccaaaataat tggtttgatt catttctgga agaaaacaca
     4321 tctttccaat ttctaaataa tctgtggttt gaagggaact gtcgggaaag agtttgcgta
     4381 tggtttgtag gggttgcgta ccccagccta attccacaac gcctaataat ttttcttctg
     4441 aaaaaatccc tagaaagtat ttacacaatc tagggagtat ctttgaataa tggtattgcc
     4501 gaataaaaga aatggcttca tccttcggaa tttctttcaa aatcaatggt gtgtttgtca
     4561 taagcaacac ctatttttca ctttcggacg cttctttttt atatttctga tgaggttttg
     4621 tctgtccttt taatacaaga tcgtctgctt tcacgtacca atcaacgtct gcccctcgaa
     4681 aagtagccgt cgctacggta tctgccacag gattgattaa ttccacttct gcgttgtagt
     4741 caaattcttt caaaggaaca gaagcgggga tactgacttg tatcatgcgt ccttgtcctt
     4801 ttgattttaa gtcataggta cgttctttaa tttcttcgga aaccgtacca tcttcattct
     4861 ggatacgaac ctcacgacgc aaggcggaaa atttcaatgt gccaaatgtc tggtcttttt
     4921 caatcacaat accttctgct aatctcatat tttttcacct ttcctttctt atgctttcac
     4981 catatcgtct gcgtgcataa tgtaattggt aaatcctcga ttgccaattt tataaccttc
     5041 tgcggtaata tgtgggttga tgagtttgac tttttcgtcg gattcaaaat gtttctcgcc
     5101 agcttcggag ggaagaatga caacaatatc atctgctcgt tgaatcgttg agtataaatt
     5161 gtaactacga gagagaatgg ttggtctgcc atttattcga cgttgttcaa tgttcccttc
     5221 accagcatat tctaattgtc caaatgtttt ttccatattg ggaatgacat atttaagttc
     5281 catattttta cctatccttt ctgtttttat gtttgaatga atcttatttg tgtgatggtt
     5341 ttaaaggggg gagagcgacc ttttgattct tgatttttta ttttcataag ttcacttcct
     5401 ttcaaaattg ggtaaaaaaa tagacgctcc gtactgagcg tccgattcct actatattta
     5461 aaacaacgac ta
//
LOCUS       DAZUMB010000148         5464 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000148 DAZUMB010000000
VERSION     DAZUMB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.152"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1135
                     /locus_tag="ACG80L_002499"
     CDS             <1..1135
                     /locus_tag="ACG80L_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024696.1"
                     /translation="LEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLA
                     LVRYVNWEGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAF
                     PLGLKEEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMEL
                     LRKDFEVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVF
                     LPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGR
                     QEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLC
                     DAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1356..3119
                     /locus_tag="ACG80L_002500"
     CDS             1356..3119
                     /locus_tag="ACG80L_002500"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS5024697.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3203..3661
                     /locus_tag="ACG80L_002501"
     CDS             3203..3661
                     /locus_tag="ACG80L_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS5024698.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3770..4453
                     /locus_tag="ACG80L_002502"
     CDS             3770..4453
                     /locus_tag="ACG80L_002502"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5024699.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4600..5214
                     /locus_tag="ACG80L_002503"
     CDS             4600..5214
                     /locus_tag="ACG80L_002503"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS5024700.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5230..5436
                     /locus_tag="ACG80L_002504"
     CDS             5230..5436
                     /locus_tag="ACG80L_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024701.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1950 a          854 c         1116 g         1544 t
ORIGIN      
        1 tcttgaattt atttctttga gcaaagtata tacaatcaaa aaaatcgtat tggatttttt
       61 taaagaaaat gattatttga aaaataaaga aatcattatc ccagaatttg actcgcggaa
      121 catcttgcta gctttagtac gttatgtcaa ttgggaaggc tatgaaaata aaaatccgta
      181 tgtagaagaa gcttgtaacg agttgattga ttatgtagag actcattttt ttaaaagaag
      241 atattcggaa gatgaaaagt cctttatcat acgaggaata gagatcgcaa tcggaagaaa
      301 ggcatttccc cttggactga aagaagaaga taagaaaagc gcagaaacga agccgttatt
      361 tcaattagtc tgtcaaggat tggaagagca aaaaggaatt ctagacttac aagaagaaga
      421 tgtctattat attttttctt tgttcaacag tagaaattat accaatgaga acatggagct
      481 gttaagaaaa gatttcgaag ttgtttataa aaattttgtt ctcaatgacc tatcactgaa
      541 tgaattgatt ttacagatta ttagtatcgc tgaaatcgac gatgcagaaa attttatttt
      601 caagcaatcc tttttacaat ttgtccgtac gttgtgggca gatggacaag tatttttacc
      661 ggaaaggatc tatcttctat cagaaaaaga acaacttctg tacggaaaaa ttgtagaaat
      721 attggaagag tggaaaaaga aaaatcaaat ccaactaaga tggaatgaaa atttcattcg
      781 aaaattcgtc aaaagtcttt ctttgatcaa cagtgaacaa gcggggagac aggaaataga
      841 aatttttgtt gtttcagaaa gttcagtcaa acaatttttt tatcgaaccc aatttcattt
      901 gtatgcggaa gaaggcatcg ctgaaataaa tcctatttta tttcgttcat tagaagaatt
      961 gcctgatgaa tgtctatgtg cgaaaaaaag agtggttttg tgcgatgctg ctagctatca
     1021 aaatggatta gaaactgaaa agacaaagat ctatccaatc tcattaaaag gtgcaaacat
     1081 ccatttgtat catattgctc aggaacttca cctattaaaa aaacatattt cttgatgaga
     1141 ccttaattag gagaccttct acaaagcttt ttaattgtct gtagaaggtt tttatttgag
     1201 tcctgattcg tgcgaagatc tacttatata acaagctttc tctgaaattt ttttaaaatg
     1261 ctattgactt agagtcaact ctaagtagta gaatagataa agtgtaacaa atctattttc
     1321 attaaaagaa gatagtacgg aggagagaat gtattatgag tacagtatta catgtattgg
     1381 atgcaatcaa tagccccgca gatgtcaaaa aattatctgt tgaggaaatg aaacagttgg
     1441 cagaagagat gcgccaaaaa atcttgcaaa aggacagcga tgtaggtggg cacgttggtc
     1501 cgaatctagg tgtagtagaa ttaacgattg cgtttcatta tgtatttgat tcaccaaagg
     1561 ataaaatcgt ttgggatgtt tcccatcagt cttatggtca taagctttta actggtcgaa
     1621 aaaatggttt tgaaaaagga cattaccgtg aagtgagcgg atattccaac caacatgaaa
     1681 gtaaacatga ctattttaca gtggggcaca catctacatc tatcagctta gcaacaggtt
     1741 tagctaaagc gagagatctg aagaaagaat caggaaatgt ggtagcattt atcggtgacg
     1801 gctccctaag tggtggctta gcttttgaag gattgaacaa tggtggtgtt ttacaatcca
     1861 atctaatcgt attagtaaac gataatgaga tgtctattga tgagaatcat ggcagtttat
     1921 ataaaaactt agctgagctt cgtcaaacca acggtaaaag cagcaataac ctatttaaag
     1981 caatgggatt tgattatcgt tacgtggata atggaaacga tctggaaacg gtgatccgtt
     2041 tatttgaaga agtaaaagat attgatcatc cgattgtact tcacgtccat acggaaaaag
     2101 gtcgcggtta tcagccggca atcgataata aaatgaaata tcattggcat gtaccatttg
     2161 atctgtccac cggggaggca aagcaagtaa cttctggaga aacatttaat atgatcatca
     2221 tggattattt ggataagaaa atgcaaacaa gtacagctcc tattgccgca gtcaatgcag
     2281 cgataccagg cgcctttgga ctgaaaacat tccaagaaaa acatccagat cattattttg
     2341 acgtggggat cgctgaacag cactctatca ctttcgcttc tgctttggct tctcagggag
     2401 tacgtccagt cgtcttccaa tcaagtactt tcttgcaacg ggcatatgat caattatcac
     2461 atgatttagc gatcaacgag aatccagtga ttattttagt cggtggcgga acgatctcag
     2521 ctagtgaccg aacacaccaa ggaacatttg acattcctta cttatcaagt atcccgaaca
     2581 tcaaatatct tgctccagca acaaaagagg atatgatctc tatgctagat tgggcgttcg
     2641 aacaaacagc gcagcctgta gcaatccgaa ttcctacaca tggtgtagaa catggtacag
     2701 ctatcgcaaa tgattatagt attccagcct atcaaacagt aaataatgga ggaaaagtag
     2761 caattttagc tttaggcggt tttcttgaat taggaaaaga aacagtaaaa gcttttagaa
     2821 aaaaaggaat cgaagcaacg ttgatcaatc caggaacgat cacagaaaat gacgtagaaa
     2881 cattagataa attaaaagaa acacacgaat tagtcatcac attagaagat ggtagtttgt
     2941 caggaggatt tggcgaaaat atcagtcgtt attatggtga ttcttcaatg aacgtattaa
     3001 attttggtgc agagaaagaa ttcacggata gcgttcctgt agggcagctt tatgaacgtt
     3061 atcatttaac accaaaacaa atcgttgaag atagtctgcg tgtcttgaat attcaataat
     3121 tcattatgct caccatccag ataattgttt atactagtat ctggatggtt ttttcattat
     3181 aaaaagcaaa ggggtggatg ttatgtatca gcaagtagaa gaatatgtca aaaagttcca
     3241 agaaaaatat cctagttatc aatcagaaaa attaggtttc ttttcatttg gggatacaga
     3301 aaaaatggca gacgagttga gcgaactagt tgtaaaaaag ataaagactg gtacatcaag
     3361 tggttacgat ctgtataaac aagagaatga gcctttacca aaagttggtc aattagacgt
     3421 ggtcctgaat ggaaaaaatg aaccagtttg tatcatcaaa aatataaatg tgaccatctt
     3481 gccttttagt gaagtcacga aagagcatgc ttggaaagaa ggagaagggg atcggacatt
     3541 agcttattgg cggaaagtcc atgctgagtt ttttaaacat gcttataaag aagcgttgaa
     3601 catagaattt acagaagaga agctggtcgt atacgaagaa tttgaagtaa ttttctttta
     3661 gtaagaaagg tctgctgaaa gaaatagctc attattgtct cctgaataga aaagggttac
     3721 atttttatat gaagctgtat ttttatataa cattactgga ggagatattg tggatacact
     3781 ggaacaaatc aaaggcggag tcgtcgtttc ttgtcaagct ttggaaaacg agccgctaca
     3841 cagttctttt attatgggga gaatggcttt ggctgcaaaa gaaggcggag cagttggtat
     3901 tcgagcaaat acgacagaag atatcaatga aatcaagcga atcgttgatt taccggtaat
     3961 agggattatc aaaagagatt acgatgggtc agaagtgttt attactgcga cagaaaaaga
     4021 agtggaagaa cttttggaga cgcaagcaga aatcattgca ctggatgcaa cttctcgtaa
     4081 acgtccgaca gaaaaaacaa ctgctgaact tgtccaaatg attcacgaac acggtcggtt
     4141 agctatggca gatatttcga cttttgagga agcgcttcaa gcgcaagaag atggttttga
     4201 tcttttgtct acgactttag caggctatac agaatattca cgaaagacgc agacacctga
     4261 ttttgattta ttgaatcaaa tcatagaaca agtcgatatc ccagtaatta tggaaggaca
     4321 tactgactct cctgaacaag tagagaaagc atatgaatta ggtgcttttt cagtcgtagt
     4381 tggatctatt atcacccgtc cgcaattgat cactaaaaga tatgtcgaag ccgcaagtaa
     4441 agcaaataac taaaaaacac gcacggcaca gattcttgac aaaaaagaaa aattgataga
     4501 tagtttaaag cagattctaa gaagatgaaa aaagcattac ttttcatttt gtcttcaaga
     4561 tctgcttttt aacgaatttt atagaaagaa ggcgaaacca tgtttgaacg gagtccttca
     4621 gaattgacag aacgtgaaaa ttacaagttg ctgattggtt cagtcatccc tagaccagta
     4681 gcagtagtaa cgactaaatc agcaaaagat atagtgaata tcgcaccctt cagttacttc
     4741 aatatcgtca gttcgaatcg acccatctta tccttggcga tccagagaaa acaagatgca
     4801 gtaaaagaca cagcaaagaa tattcttgag acaagagaag ctgttatcca tatccttgat
     4861 gaggacaacg tagaagaagt aaataaaaca gcagccaatc tgcctaatga ggaaagcgaa
     4921 ctgtacctga ctgacttgac tttaactgat tcagtaatca tttcagtacc agcattgaca
     4981 gaagcaaaag ttcgttttga aacatcctta tatgaacatg tcgagatacg gaataaaaat
     5041 aaagtaacag ctgatttatt tttgctagaa gtacagaaat atcatttaaa tgaaacagtc
     5101 tatgatgata agacaggaag gatcgatcca agaaaattga atgctgtaag ccgtttggca
     5161 ggaaatgatt atgcagcaat aggagaaatt ttcacgattg cacgtcccga ataaaaagga
     5221 gcactagtta tgttaaaaat aagctataaa ccatcgacag attcaaaaga aatgaaaaaa
     5281 gaatatgaaa cagtgaatga ttttctgcaa gggcagtact tagaagttcc gccattgcaa
     5341 gaccattttg tcgttactac tgttacttta gatggtaagg aaatagaaat gccagatcaa
     5401 acgattagcg gattgtttaa ttactttaac aaataaatat ttattccgaa aagtgaaaaa
     5461 acat
//
LOCUS       DAZUMB010000149         5425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000149 DAZUMB010000000
VERSION     DAZUMB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.153"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACG80L_002505"
     CDS             complement(152..550)
                     /locus_tag="ACG80L_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS5024702.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACG80L_002506"
     CDS             complement(547..1320)
                     /locus_tag="ACG80L_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5024703.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACG80L_002507"
     CDS             complement(1317..2000)
                     /locus_tag="ACG80L_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5024704.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACG80L_002508"
     CDS             complement(2167..3054)
                     /locus_tag="ACG80L_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5024705.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACG80L_002509"
     CDS             complement(3051..3920)
                     /locus_tag="ACG80L_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5024706.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACG80L_002510"
     CDS             complement(4057..5343)
                     /locus_tag="ACG80L_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5024707.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1665 a         1211 c          905 g         1644 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttatt
//
LOCUS       DAZUMB010000150         5289 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000150 DAZUMB010000000
VERSION     DAZUMB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.154"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..339)
                     /locus_tag="ACG80L_002511"
     CDS             complement(19..339)
                     /locus_tag="ACG80L_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5024708.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(487..2721)
                     /locus_tag="ACG80L_002512"
     CDS             complement(487..2721)
                     /locus_tag="ACG80L_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5024709.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3200..3466
                     /locus_tag="ACG80L_002513"
     CDS             3200..3466
                     /locus_tag="ACG80L_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS5024710.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3466..5193
                     /gene="ptsP"
                     /locus_tag="ACG80L_002514"
     CDS             3466..5193
                     /gene="ptsP"
                     /locus_tag="ACG80L_002514"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS5024711.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         1595 a          984 c         1066 g         1644 t
ORIGIN      
        1 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
       61 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
      121 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
      181 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
      241 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
      301 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
      361 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
      421 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
      481 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
      541 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
      601 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
      661 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
      721 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
      781 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
      841 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
      901 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
      961 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     1021 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     1081 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     1141 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     1201 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     1261 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     1321 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     1381 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     1441 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     1501 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     1561 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     1621 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     1681 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     1741 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     1801 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     1861 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     1921 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     1981 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     2041 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     2101 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     2161 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     2221 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     2281 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     2341 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     2401 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     2461 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     2521 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     2581 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     2641 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     2701 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     2761 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     2821 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     2881 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     2941 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     3001 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     3061 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     3121 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     3181 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     3241 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     3301 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     3361 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     3421 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     3481 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     3541 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     3601 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     3661 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     3721 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     3781 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     3841 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     3901 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     3961 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     4021 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     4081 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     4141 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     4201 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     4261 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     4321 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
     4381 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
     4441 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
     4501 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
     4561 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
     4621 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
     4681 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
     4741 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
     4801 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
     4861 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
     4921 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
     4981 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
     5041 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
     5101 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
     5161 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
     5221 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
     5281 tagacaaaa
//
LOCUS       DAZUMB010000151         5261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000151 DAZUMB010000000
VERSION     DAZUMB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.155"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..354)
                     /locus_tag="ACG80L_002515"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="ACG80L_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="ACG80L_002516"
     CDS             complement(614..1162)
                     /locus_tag="ACG80L_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024712.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="ACG80L_002517"
     CDS             complement(1163..2020)
                     /locus_tag="ACG80L_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5024713.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2593)
                     /locus_tag="ACG80L_002518"
     CDS             complement(2306..2593)
                     /locus_tag="ACG80L_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5024714.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2583..2912)
                     /locus_tag="ACG80L_002519"
     CDS             complement(2583..2912)
                     /locus_tag="ACG80L_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5024715.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2989..3054)
                     /locus_tag="ACG80L_002520"
                     /pseudo
     CDS             complement(2989..3054)
                     /locus_tag="ACG80L_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            3090..3659
                     /locus_tag="ACG80L_002521"
                     /pseudo
     CDS             3090..3659
                     /locus_tag="ACG80L_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            3794..4346
                     /locus_tag="ACG80L_002522"
                     /pseudo
     CDS             3794..4346
                     /locus_tag="ACG80L_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015474649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4498..4731
                     /locus_tag="ACG80L_002523"
     CDS             4498..4731
                     /locus_tag="ACG80L_002523"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5024716.1"
                     /translation="MFQSFIHEKQRELSIKMIQALKTFRQSEEAIVNALNYHYSNGLV
                     EGINNKIKVIKRTAYGYRNFSNFRNRIFIEREL"
     gene            4816..>5261
                     /locus_tag="ACG80L_002524"
     CDS             4816..>5261
                     /locus_tag="ACG80L_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS5024717.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDSDTLPMKGKRKPNGHQEKRGKQQYQRSIHDR"
BASE COUNT         1652 a          996 c          841 g         1772 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccagtc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ccccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagg aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttatttga
     3061 tagtagattt aaaactttgc aacagaacca ttgtagctat tggggcaaag acttattcag
     3121 ctcactcgat tgccggcagc attgaaagtt ttgtttatat gccagcttat ggattagcta
     3181 ccgctgcagc aactttagca ggaaatagta ttggaaaaaa agactatgtg gaaacaaaac
     3241 gtgttgcatt ttatgcggtt aaatacggcg tgattgtttt aagtatccta ggtataggat
     3301 tgttttttgg tacgccatat attgcacctt ggtttaccac agatgctgag gcaataaagc
     3361 aaattgttat tgcgctaaga attgacgctt ttaatcaacc aggactagct atcagtctaa
     3421 tattagctgg cgtactccaa ggaatgggag atactaaaac cccattatac agtactgcat
     3481 ttggaatgtg ggtcacacgc atattaggtg taatactttt agggaatata ttggatttag
     3541 ggattgctgg agtgtggtta gcaattggca tggatctata tatacgttca ctttttttaa
     3601 tctatcgttt cagacgaaat atccaaattt taaataacaa taacgaaaca ccttcctaaa
     3661 ccagtgattt tttatcgctg gtttttgcca tttagcgatt aaaaaaattg ccatataaat
     3721 agaaaagtaa cacaaggggt acaatggtgt cataactaat ggtcataagt gaagtgaaat
     3781 aaggggtgaa aacatgaaat atggctatgc acgggtgagt acccgccacc aagatctgga
     3841 agggcaaatg cgtcagctgg aagaggaacg ctgtgataaa attttctttg aaaaaataac
     3901 gggaacgaag agtgatcgtc cagaattcca aaaactgctt caggaagttc agactggtga
     3961 tacgttagtg gtcacaaaat tagaccgatt tgctcgtagc acgcaggatg ctcttcatac
     4021 aatcaagctt ctttttgaaa aaggcgtgcg aattaatgtt ttgaatttgg gcattattga
     4081 gaacacgtct actgggcgat taattattca ctatttttag tgcctttgcg gactttgaac
     4141 gcgatttaat tgtggaacgg acacaagagg gaaaagaaat tgccaagcag cggcccggtt
     4201 ttagagaagg gcgaccaaaa aaattttcca aacaacagat gactttagca atgaaactac
     4261 tagagacaaa ttcctataaa gaagtagaga aaatgactgg tattagcaaa agtactttaa
     4321 cgcgttataa aaggaagaag agttaggagc cacagaatac cgatatcatc gtcttttcaa
     4381 aggcatggtg acggaaaggg gaatcgttga ttattttctt tccttggacg aaggtttacg
     4441 ccgccattac aacgtctacc aaacaactgt ctttgccatt aactatcgga accgaaattg
     4501 tttcagtcct tcatccatga aaagcaacgg gagctttcaa ttaagatgat tcaagcctta
     4561 aaaactttcc gacaatcaga ggaagccatt gtcaatgcgt taaattatca ctactccaat
     4621 ggtttagtgg aaggtatcaa taataaaatc aaagttatta aacggacagc ctatggctac
     4681 cgcaactttt ccaattttcg taatcgtatc tttattgaac gcgaattgta aaattaagtt
     4741 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
     4801 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
     4861 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
     4921 caaactattt ataatgtagt caataagttc aagcaaggta agactgctct tgattattgg
     4981 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
     5041 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg
     5101 cgaaaagaaa gacctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga
     5161 atatttgata gtgacacact accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa
     5221 aaacggggaa aacaacagta tcagcgctca atccatgatc g
//
LOCUS       DAZUMB010000152         5220 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000152 DAZUMB010000000
VERSION     DAZUMB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.156"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACG80L_002525"
     CDS             complement(<1..1256)
                     /locus_tag="ACG80L_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5024718.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2526)
                     /locus_tag="ACG80L_002526"
     CDS             complement(1270..2526)
                     /locus_tag="ACG80L_002526"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5024719.1"
                     /translation="MKRGYKVRWRCSNMSNDTRPEIRFPGFTEDWEERKLGDMMDVTS
                     VKRIHQSDWTNSGIRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLL
                     VTGVGSIGVPLLITDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAG
                     IGTVGTYTIDSGKKTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQ
                     KMFPKNGAKVPEVRFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNF
                     NTNDRWYYSNLELEEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKEL
                     DKQYLYTWLLTDKEKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLD
                     DTIALHQRKLDLLKETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="ACG80L_002527"
     CDS             complement(2477..4072)
                     /locus_tag="ACG80L_002527"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS5024720.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="ACG80L_002528"
     CDS             4197..4982
                     /locus_tag="ACG80L_002528"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFS5024721.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            4975..5217
                     /locus_tag="ACG80L_002529"
     CDS             4975..5217
                     /locus_tag="ACG80L_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024722.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAZUMB010000153         5159 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000153 DAZUMB010000000
VERSION     DAZUMB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.157"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1214..1879)
                     /locus_tag="ACG80L_002530"
     CDS             complement(1214..1879)
                     /locus_tag="ACG80L_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024723.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2059..4041
                     /locus_tag="ACG80L_002531"
     CDS             2059..4041
                     /locus_tag="ACG80L_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS5024724.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4045..4683
                     /locus_tag="ACG80L_002532"
     CDS             4045..4683
                     /locus_tag="ACG80L_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024725.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4711..5139
                     /locus_tag="ACG80L_002533"
     CDS             4711..5139
                     /locus_tag="ACG80L_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024726.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1698 a          787 c          945 g         1729 t
ORIGIN      
        1 ttgaccagat tttgaccagt ctaatttaca ttctaatata ttcatatgag ctaatttttt
       61 gattaaatgg taagtttttc cttgtttgtt ttagtttaaa ctagtgtgta atttaaaaat
      121 ctatatggaa gggtctgtgg ggtaatactt aaataaaggc taggaacctg ataacatcaa
      181 ggttcctagc ctttttataa taaaatgaag cgaaaataat ttcttttaaa gagccgagat
      241 tatcagctat ataagagtga atttgatgca aaaaacagag ccgaaacagt cttggtgttc
      301 gtagctctgc ttatatgttt cgaatttaag acaaagagtt ggcaaaagat gttgataagg
      361 aagataagat aactgtgtta tataaactag gtaactaact gtaacaagct aattttaaga
      421 gattcttatt agaagacgtt taggaatata actaagaaaa tgattataca ggtaattgtc
      481 atttcaataa tgttttctaa ttttcgcgtc tttgttttaa gaaaaaggcc agaatcagtt
      541 ggaaaaaagt tcgtacgata gtgaagaggg gcaaaatttc ctactaatat tcccactaac
      601 aagagataaa acggccaggt tggtgtgatt cctctataaa aaaatgccca tgctaccgaa
      661 atcccataga ttaacaactg aacaattttt acaatgaaaa aattgttctg ccattactca
      721 aagtctcttc ctccttgtat cgtttatttg tttcatttta tcattatgat aggcgcttga
      781 acaatccatt ttgtatttta ttgagtactc ttggataaac taccagcgat tatcatattt
      841 atttatgtta ctgtaagcca tcatataaaa gtaaatgagc agatcataga aatataaaaa
      901 agaactaaaa ttgatctaaa ttatgtgcag cttgactacg taccaatcta tattacttac
      961 tagattggtc gttaatctat tcggatcgaa tgagcaaaat atttttgtga atgtccatcc
     1021 ttgattatga ttccaaaaag aatcattggg aggctgtggc gtaaatatta tactataagc
     1081 cagaaatacc tatcattctg aactttttta tttaggaact ttcatctcaa ggctttttca
     1141 attaatttgt acccctgttt tttggataag atcatgtgca ttgatttttt ctatctctac
     1201 gttcatctcc tacttaatgc ctatctggct cctgagggct tctttttaag gcgtcgtcat
     1261 agtcttggct gaggatttct tggtaatctg agggaatggc ttcccaagtg agcaggcgaa
     1321 cgttcatgac gtgttttcct ttgtgtttga ttacagcatg gtttttctct ttttctacct
     1381 ctccagcgtg aaaagacagc catgtaccat ggccaaagat atcattacat acccaacgtt
     1441 cgatggaatt gtccacgtat cctacaggga gtgtggtata gccgatttgt ggtttcaaaa
     1501 aaggattgaa tcgatcgatc gtttcatttt tgtattgata attgataaga aaaagataga
     1561 ccaaacaaaa gacgagcaga aagaaagata gtgtcttttt gagtgagaag ggttgttttg
     1621 atgaataaga tttcttgtta acaagaggtt catctggcaa tgttggttct attgattcgg
     1681 tagctgattc aatagaagga aaatcaatcg gcagacctaa agtatgatgt aaaagtgaca
     1741 gcatttcaag gtctggcatt gtacgagaat tttcccaact agaaatcgtt ttatcggaaa
     1801 caccaatttt ttcgcctaat tctttttgcg taagcttaca atttctgcgg taagttcgta
     1861 ttttttctcc ggtgttcatc ttcttctcct ttcgattcta cagtatatag atttattcta
     1921 catgacctat acacttgata gatacgtttt tttgatgaaa aacggtatga ttttatcaaa
     1981 gaatcattta agtaagggag agtggtgata atcaaaaaaa agttcacagt tttactcaca
     2041 atcatagtga ccggtatcat gagtggtttt gccttggtta gtataaataa acaggtactc
     2101 tatcggacaa atgaattttt ccatcaaaca gatacttata ctccgattcg attgccacaa
     2161 cattttattg ggcaagaaga atatgagcag ctaatcatgg tatttgaaaa tgtctcatca
     2221 gaaactgagc tttcttatat gaaaaaaaca gtaaatgaat ccaatgaagt agatacatct
     2281 tggaacttca agatacagcc aaagcttgaa atgacattgt attatcaagg ggatgtcaaa
     2341 tcttctcacg agaaggtacc ctattctaat acctcctttc gcgtaacgtc cattgaagaa
     2401 tcgatcaaaa taaaagattt tgaaggtgaa tttttcttga tgacgagaga aaaagaagcc
     2461 tatgaacggt tcatttcact attcactgat cgattcaatc aagcatttca cactgattat
     2521 gccactaata tttttgaaga ctttgaaaat agctattcgt attcggttat ttcaaataca
     2581 gacgtatata attatcgagt gtatttaact attggattga ttcttttatt ccttttgtta
     2641 tcatcctttt tcttattgat gaataaagaa atcagtttat tgttgaataa tgacttttca
     2701 ttacatcaag tattgtggtg tttattgggg aaaaagaaaa tcattattac attcataatt
     2761 gcaggaatca ttggtggttt tatttttatg tattataagg agcaatttct ttatttgttc
     2821 cagatgtacg ttttcttgtt cagcggaata tatggtatct ctttgatttc tcttttatta
     2881 gttgaaaaaa tcgcttcgtt aagagaagcg ttcaactata tccaatacat tctttttaca
     2941 tttggattgt tgttttctct tcttttgacc gttgccaatg tcgaattagc aactatcgtt
     3001 ttttcttctt cacagttagc tgtttcccat gaaccaccaa aagaaatcgc tggtgaggac
     3061 tatcatgtat tttatccagt aaccatcggg aaaaaccacg tggattttat ttattccaat
     3121 cgttttgcat cagcagcaga tcaggaatta tatgaaactt tgaataaaaa cggaagtata
     3181 ttagtcaatg tatcagatta cttgaatgaa gaaaatgaac agtttggcag agggataaaa
     3241 ataaatctga attatcttaa gaaatttcct ttgattgatg tctctggaag gttgatccaa
     3301 ataaccgaaa aagaaacgca ccctgttata ttgatccctg aacgatatag acatacagat
     3361 ttaaaaaatg acctggcgca aataacagaa tattatcaag aaatcagtga aaaagaaccg
     3421 aaatttctat ttataaaaaa caatcaaccc atttatacat ttattccttc tatcccctgg
     3481 atcgaacatt atccggttgt tgaaatactg acattgaaaa acagtatgta tttggaaaga
     3541 aatattctca gcggagaaat ctatccaccg ttaaagatca agataggctc tttgtcgaaa
     3601 gaaaggttat ttgagctgat tgaagaaagt cagttacgtg ataacttgca gttatcattt
     3661 ccgtatactc agactgaaga gatagctgtc aaagtgttaa gcagatcatt tcattacttg
     3721 atacagatat ttttactcac tttcttttct ttcttcttgt tgagctatgt catgttatgc
     3781 atatattttg tctacaattg tcggaaaatc gctatctttc gttcaagtgg ttattcgctc
     3841 tttgaaacgt ataaagattt cttcatgatg aacctcatca aatggggaac gacaagtgta
     3901 atcttccttt ttcttataga aagagagcct aaatatcttt ttaatatttt tttctttagc
     3961 ttcattgatt ttattctttc agttgttttt attttgtcta cagaaaagaa aagtcagtta
     4021 ttgctgatga atggaggtta acaattggag ccgttgatag tgataaaagg tgcgaaaaaa
     4081 atcattcgaa acaaaacgat tcttcatttg gatcgtttca ctgcggatgc tggggatctt
     4141 gtgactgttg ttggagaaag tggagcaggt aaaagtacgt tattgaatat attaggattg
     4201 aatgatcagt tcagttcagg acaattctat ttgttcgatc aagagacgaa acaattaact
     4261 gggagagaga agcgcttgat aaaaagaaat accatatctt ttctttttca agattttggg
     4321 ttgattgaat atgaaacaat cgattttaat ttagatattg gattgaagtt ttcgaattta
     4381 aatgtttcag ataagcgaac agcaaagtat gaggcattga aaaaagtaaa tataaacaag
     4441 aagctaagta cgcctatcta cacattatct ggtggtgaga agcaaagaat cgcctgtgcg
     4501 agagtcttat tgaagaatag caaaatcatt ttagccgatg aaccgactgg gtcattggat
     4561 aaaagaaata aggataaagt agttgaaatc ttatcgcaac aagcttccca aggtacgtta
     4621 gttatcgtag tcacgcatga tccatatctg accaaaatag gaaacaagaa gatcatgctt
     4681 taaaaatcaa aaaggaaagg atgtcgtttt atgaaaaaag cagttattat ttgtgcacta
     4741 gggataggtt ttgttggagg aaacgctatg tcatctgaag tggaagcaaa ttcagttcac
     4801 ggtgaaatct attacaaatc tttaaagcca ggagagattc attttgatgc taaaggtaat
     4861 attattaagt caaatttacc agtagaccgg gaaatttcta gaacagcaat taatcatagc
     4921 tctggaagat gggtttatta tagtgataca acttgtacga gaaaaacagg tcattctaac
     4981 cactatagtc gtgtctataa tcgtcacggt gcaaaagcga aagttggaaa ctctattcga
     5041 tcagcaaatg caacaaaagg agtatgggcc tatgcaagtg cgacaggaaa tagaactgac
     5101 acattctcat gttggtacaa tccaaccggt tggtattaag tatccaaaag tttatataa
//
LOCUS       DAZUMB010000154         5102 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000154 DAZUMB010000000
VERSION     DAZUMB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.158"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            169..1884
                     /locus_tag="ACG80L_002534"
     CDS             169..1884
                     /locus_tag="ACG80L_002534"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024727.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1887..3650
                     /locus_tag="ACG80L_002535"
     CDS             1887..3650
                     /locus_tag="ACG80L_002535"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024728.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3690..4226)
                     /locus_tag="ACG80L_002536"
     CDS             complement(3690..4226)
                     /locus_tag="ACG80L_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5024729.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     regulatory      complement(4264..4362)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(4549..4971)
                     /gene="mgsA"
                     /locus_tag="ACG80L_002537"
     CDS             complement(4549..4971)
                     /gene="mgsA"
                     /locus_tag="ACG80L_002537"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS5024730.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1533 a         1023 c          980 g         1566 t
ORIGIN      
        1 attttctttt ttttaaaaaa aatagtctat tcaatttcta aagcttcttc ataataaaac
       61 tttcatttat gaaaaggctt gttctctttt cattctaaat ttcctaagct acaatgtggt
      121 agtacttgtt ttttcaattg tggacaaata aataatagga ggaatcatat gattcagtta
      181 ttacgttatg caaaagacta tcgtaagcaa atcattttgg gcccgttctt caaatttctt
      241 gaagctgtct tcgaacttgt cctgccttta atgatggctt ccttgatcga taatggacta
      301 aaaatgaatg accgaggaaa aatcattgaa atgggcttat ggatggtcgc tatgtctgtg
      361 attggtctga tttgtgctat catttgccaa tactatgctt cagtagcttc acaaggattt
      421 ggtacagagt tacgtaatca gctaatcaaa aaaatcaata ctttctctca caacgagttg
      481 aatcattttg gaacagatac cttgatcacc cgaatgacaa acgacatcaa tcagcttcag
      541 ctagctttag caatggtcat tcgtttattg atcagagcac cgtttttaag tatcgggtca
      601 gtggtcatgg cttttgtgat cgactgggaa gtaggtctgt ttttcttagc attattgcct
      661 attttctcaa tcattttatt ttttatcatc aaaaaaaccg ttcctctcta ccaaaaagtg
      721 caagaaaaac tagatcagct caatgaacac gttagtcaga atcttagcgg tgttcgtgtc
      781 atccgcgcat ttgcgcagac aaaaaaagaa acagagaagt tcgataaaag cacaaatgat
      841 ctagcgaaca attatttacg tgtttccaat atctctgccc tattatcacc tgctacaacc
      901 ttgatcatga atgtcgggat catctgtttg ctgacagttg gcgggatcaa agtaaatatt
      961 ggtagcttgc aacaaggaca agttcttgct ttgatcaatt atatgaatca aatgcttttg
     1021 gcactgatcg ttgtttcaaa cctcgtcgtg atcttcacac gtgccgaagc ttcaggaaat
     1081 cgggtgaaag aggtgctaga cacagaaaat tcgattcttg aagctgaggt cagtactact
     1141 cccgattttt cttctgaagc aattatcgct tttgaccatg ttgacttccg ttatactcct
     1201 gattctggtc tttctctcca aaatattaca tttcagctga agcgaaatac agtccttgga
     1261 atcattggtc caaccggaag cgggaaaacg actttgacac aattgattcc gcgtttctat
     1321 gatgtcagtt ccggtacagt tataatggac ggaatggatg ttcgttcttg gcagctggat
     1381 catctccgtc agcaaattgc tcttgttccc caaacttctg tactatttac cgggactatt
     1441 cgtgaaaacc ttcaatgggg taagaaagac gccacagaaa aagaatgttg ggaagctctg
     1501 aaaatcgctc aggcagaaga atttgtccgt cagcttccaa atggtttaga tacaaaagtc
     1561 atggaaaatg ggaaaaattt ctctggcgga caaaaacagc gactgacgat tgcaagagct
     1621 ttgattgcca agccagcatt actgatttta gatgactctt taagtgcgtt ggattatcaa
     1681 acagacttgg atttgcgtaa ggcgctacag acttatcttg gcagtacagt gattattatc
     1741 tcccaacgtg tccgctctat tcaagaagca catcatatct tagtgatgga tcaaggtaag
     1801 attgctgctc agggtaccca tgaagaatta cttgctcaat cagcagaata tcaggaaatc
     1861 gtcgcatcac aggaggaaga atagttatga aagtaaaaaa attatccttg cagacgctcc
     1921 gccgattcat tccttacctt actgcctatg caaaagacat ttggctagct gtgatactag
     1981 gaatcatcaa tgggattgca acagttgtga tgacgttaca aattggtcaa agtatcgatc
     2041 aaatgattgg agaaggacag gtccgcttca atcgactgtt ccatttactt attctattcg
     2101 gtggtatcgt tttaatcaac gtagtcagtc aatggctcat ccaagtgtta agcaatcggt
     2161 tagcttactt atccgttgca aaattaagaa aagatgcatt tgctcatctg aatcgattgc
     2221 cacttcgtta ttatgatcaa aactcccacg gaaatatcgt cagtcgattt acaaatgata
     2281 tggacaatat ttccattgca atttctgcgg ccttcaatca attattttct ggagtggcaa
     2341 tcatcattct ttcgtttatt tttatgatgc gtctaagtcc acttctgaca ttggtcgttc
     2401 tttgttctac accaattatt ttccttgtga gttggatcgt tgctcgtgct tcacaaacaa
     2461 atttcgatag acagcaaagg attattgggg atatctcggg ctttgtgacc gaacgaatcg
     2521 gtaaccagaa aatcgtgaaa gcttttcagc aagaagaagc tagtcaacag caatttgaac
     2581 tgctaaatgc tgatctatat gaaaaaggac aacgggcaca attttcttct tccttgacaa
     2641 acccgtcttc tcgttttgta gatcaccttg cttatttatc gattggttta gttggcggcc
     2701 tattggcatt gaagagtggt tcgtcaatca ctgttggtgt gatttccagc tttacgatct
     2761 actcaagtca gttttctaaa ccatttatcg aactttctgg aatcacgaca caactgcaaa
     2821 ccgctttttc tggtctagaa cgagcatttg atttgatcga tcagccagaa gaaaagcctg
     2881 atttgccaga tgcctatgcc ttggaaccca aaaatatcaa aggaaaagtc tcttttgatc
     2941 atgtttcttt ttcttatgac cccaagcagc gtttgatcga agatttcaat tttactgctt
     3001 atccaggtca aaccattgct atagtaggaa aaacaggggc cggaaaatcc acacttgtta
     3061 atttgctgat gcgtttttat gacacaacaa aaggaacaat tacagtcgat gaccatgaca
     3121 tccgctctat ccaacgtgat acattacgta aaagttttgg aatggtgtta caagatactt
     3181 ggctatttga cagtacattg cgagaaaatc ttcgttttgg gaatccggaa gcaagtgacg
     3241 cagaaatcaa tgatgcccta aaagcctctt atatgtatga atttgtagaa cgtttgccgg
     3301 aaaaattaga tactaagatt ggcggtcaag ggttaaagat atcagatgga caacgccaac
     3361 tgttgacgat tgctcgaacg atgatcagta atccccctat gttgatctta gatgaagcta
     3421 ccagctcagt cgatacattg acagagaaaa aaatccaaca agccttctta gcaatgatgg
     3481 aaggaaaaac cagttttgtg attgcccatc gcctgtccac aatcaaaaat gcggaccaga
     3541 tattagtgat ggatcaggga gctattgtcg aaatgggcac ccatgatgag ctgcttcaaa
     3601 aagatggtta ttaccgacgg ctctatcaag cacaattcac agcacaataa aaacaagcag
     3661 tcttctggga tgggaagact gcttgttttt tatttagcgt ggatcgctaa acttcttaaa
     3721 atttttttgt aaggaatact acgtcctacg taataaatgg caattgattg aatcggtaac
     3781 catattactg tttttaacag gcggggcgcc caaacgaccc agctgaacaa tggcacatgg
     3841 tacatcaacg caagccataa tggtgtcaaa aataagttga tgatcagcgt gacactaagc
     3901 gtagccagaa tggcattttt ccaagtgatc tcttttcgat agaaaaagaa tccgtagatt
     3961 gctccttcaa taaatgcatt caatgtaaat ccaataaaga atgcagattt cgagaagagg
     4021 gccattccta ctaaatcagc taaaactgcg cctatccctg accaaaaagg accaaaaaga
     4081 atgcccatga tcacttgtgg aataaatgtg acactgattt taataaatgg ggtctcgatt
     4141 gctaccagtc gggacaaagt gaccatcaat gcaatcaata agcccataat agcaatcata
     4201 cgtgcgtcta accggtgttt cttcattttt agcatctcct ttaagagctg ctacatcgta
     4261 tcatgcagcg aatgcgaaaa taaaaccgcg aacattacgt ccttcgtcca agaggcaaca
     4321 tcccatcctc ttagcactta acgctttatt ttctactctg tattattttt gggatctttt
     4381 cccacagaaa ctatagcata ttttcacatt tactgcaaat acgaaaaact aatattattg
     4441 tttatattat ccaatacgtt cgtattatat cgcatattat cagtttgttt agcttttata
     4501 gctacatata ggcgaacatt aaacgttatt tttccatacg cttatccatc aaaatgaaag
     4561 tggatgcttg tctatttcat ggacgattct tcgaaaatct aggaagccgg cttccagtcc
     4621 tcttagtagc acctcagctg ttccgatatt cgtagctaac ggaatctcat agacatcaga
     4681 taaacggata agtgctgtta catctggttc atggggctgt gcagccagcg ggtctcgaag
     4741 aaaaatcacc aaatctaact gatcctccga aatcatggct ccaatttgct gatccccgcc
     4801 taacggtccg gatttaaacc gatggatcgg caagccggtt gcttccgtga tcctctgccc
     4861 agtcgtcccg gtagcaaaaa gttcatgttt ttccaatatt gttttatacg ctgttgctaa
     4921 tttcaccatc ataggttttt ttcggtcatg agcgatcaaa gcgattttca taagagacac
     4981 tccttttgat attgttaaca tacttattta ctccagtgta tcagttttta tttcttatgg
     5041 atatttatta aagcttaaaa ctagcacatt gacaaaaaaa agaaaaaatt cttgttcaac
     5101 at
//
LOCUS       DAZUMB010000155         5061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000155 DAZUMB010000000
VERSION     DAZUMB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.160"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..1179
                     /locus_tag="ACG80L_002538"
                     /pseudo
     CDS             38..1179
                     /locus_tag="ACG80L_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1305..2384)
                     /locus_tag="ACG80L_002539"
     CDS             complement(1305..2384)
                     /locus_tag="ACG80L_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5024731.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2487..2927)
                     /locus_tag="ACG80L_002540"
     CDS             complement(2487..2927)
                     /locus_tag="ACG80L_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5024732.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2984..3394)
                     /locus_tag="ACG80L_002541"
     CDS             complement(2984..3394)
                     /locus_tag="ACG80L_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5024733.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3574..4008)
                     /locus_tag="ACG80L_002542"
     CDS             complement(3574..4008)
                     /locus_tag="ACG80L_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5024734.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4108..4956)
                     /locus_tag="ACG80L_002543"
     CDS             complement(4108..4956)
                     /locus_tag="ACG80L_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5024735.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1845 a          971 c          845 g         1400 t
ORIGIN      
        1 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
       61 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
      121 gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
      181 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
      241 catcaaagca aaaattgact attcgggaga cttagcacct atactacaat cagctgttac
      301 atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt tgactatcca
      361 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
      421 gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa cgccgtatag
      481 tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc ttgttgaaga
      541 accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga
      601 cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga
      661 gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg taactccaga
      721 accagatacg gattctggaa accaaacagt accggaaaca aatccagaca cagataatga
      781 aactgaaaat ccagaaaaac cagaggtaga tccagaagaa aaaccagatg tgactccaga
      841 accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa aaacaattca
      901 agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa taaatgaaga
      961 gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa ccttaaaaag
     1021 tagcaaggat acaacctttg ctgtcctgaa acgaaaaata aacagctacc aaaatccgga
     1081 gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat agcaacaatg
     1141 ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa tgtctgtgaa
     1201 cgcatttttc aaataaaaag aaatatccag aaaatgaccc caaaatacca gattgaaatc
     1261 tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg gatatctgtt
     1321 tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa gaaggtaaag
     1381 atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac aagtaagacc
     1441 acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg attcagcaca
     1501 taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac atctcggatg
     1561 cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac acctgctact
     1621 gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg aaagccaaat
     1681 acagccaagg cgattgtagt gagcaacgta tttaaaagag cgacaacaaa ttgagcttct
     1741 aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc ttgaaagaac
     1801 caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt cttttcaatc
     1861 ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt tcctatatcc
     1921 tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc
     1981 tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa
     2041 aaattaataa cggactggat catgtcaaat gtttgatgaa taagcatcgg gatatacttt
     2101 gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc taaaacacct
     2161 gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag
     2221 ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac aaaaacgaca
     2281 aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg ataaacggtc
     2341 aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg atggaatatg
     2401 aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga gttggttttc
     2461 ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg aaaattatct
     2521 agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag tggaagataa
     2581 gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc ttgagcagtc
     2641 agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat ataatttttt
     2701 tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa atcatttcct
     2761 agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag atactgggta
     2821 taggtaagat tcagcggctt gaggagggga gtgtagtgtc taatgatttc tttagaggcg
     2881 gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt tccacctccg
     2941 ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca ctaactgctt
     3001 tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact ttatgcgctg
     3061 cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct tcaatctcta
     3121 cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca ctgacagtag
     3181 aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta aaacatgcac
     3241 tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag
     3301 ctgcaatatt caattcgaat gtacgatcgg gattatgcac ttctccagag cgtccgcctg
     3361 tgttgatcat tttcgtagaa tacatttttt tcataaaatt accaccttta attatatttt
     3421 atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt ggcataagat
     3481 aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa
     3541 tatcagagac aaaaaataat ctcatcataa acattacata tatttctcaa aaagttgttt
     3601 tctttcttca aaaattactt cacgatggac ctgatgaagc atccataaat aaccaaaagg
     3661 atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt cttgaatctc
     3721 tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga cgatgatatt
     3781 gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag acgtgttttc
     3841 atccagcata tggaatctcg taccatacaa ggtaaaaatg acttcattat tgcctctagg
     3901 gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca gttctagtgc
     3961 tttcaaacta tcatttaata caaaatcgat ttccgttcct ttatgcataa agatctcctc
     4021 tccttttcag tcgaacagtt actagattct tttattatac cagctaaaga aaacgcattc
     4081 aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa atccgcgtcc
     4141 cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt cttctatcaa
     4201 tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct cttttgactt
     4261 tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct cagcacttaa
     4321 gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt tctttcgatt
     4381 gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata aaatagcata
     4441 aagaaactct tctattccaa aaataaacag attaagagaa acaacgaatg cgtctgtaaa
     4501 aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg gagggatcgt
     4561 cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag caaaaatcgc
     4621 acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg gaactattgc
     4681 aatgattaaa ggaaaaacca agcttccgaa aagtacttta aaaaagggtt ttttacctaa
     4741 aaatagaaga gctagtacca acataacgat attcaacact aaaataacta tagctcggtc
     4801 aaagcctaaa gcagcttcca gtaatattcc tagaccgctg acacctccag cagcaatatg
     4861 atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa aaataatctt
     4921 aatgtatgtc catataaatt gtctcgtttc tttcatttat tcacttcctt aattcattaa
     4981 taatactctt atcttattat aacttacttt tcaccttaag ttaactttaa ttaataaaaa
     5041 aattaggcta gtctaaatta t
//
LOCUS       DAZUMB010000156         4994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000156 DAZUMB010000000
VERSION     DAZUMB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.161"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..877
                     /gene="purR"
                     /locus_tag="ACG80L_002544"
     CDS             59..877
                     /gene="purR"
                     /locus_tag="ACG80L_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS5024736.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1380..2753
                     /gene="glmU"
                     /locus_tag="ACG80L_002545"
     CDS             1380..2753
                     /gene="glmU"
                     /locus_tag="ACG80L_002545"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS5024737.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2847..3818
                     /locus_tag="ACG80L_002546"
     CDS             2847..3818
                     /locus_tag="ACG80L_002546"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5024738.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3824..3926
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3992..4561
                     /gene="thiT"
                     /locus_tag="ACG80L_002547"
     CDS             3992..4561
                     /gene="thiT"
                     /locus_tag="ACG80L_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS5024739.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4642..>4994)
                     /locus_tag="ACG80L_002548"
     CDS             complement(4642..>4994)
                     /locus_tag="ACG80L_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024740.1"
                     /translation="DEFYRIFASKLSSILSDKINVKKSFFKKETPVEKISVELLKEMR
                     NLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSVTDRHW
                     QLVEDADLSYLPLK"
BASE COUNT         1634 a          880 c         1082 g         1398 t
ORIGIN      
        1 ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt
       61 gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca
      121 tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag
      181 tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac
      241 aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa
      301 aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg
      361 atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat
      421 cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg
      481 cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg
      541 ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga
      601 aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt
      661 agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga
      721 attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg
      781 cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa
      841 tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag
      901 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc
      961 ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc
     1021 tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga
     1081 gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt
     1141 ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg
     1201 tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat
     1261 ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg
     1321 aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt
     1381 tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat
     1441 tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg
     1501 tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta
     1561 aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac
     1621 atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca
     1681 gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag
     1741 gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac
     1801 gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac
     1861 cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt
     1921 tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag
     1981 atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt
     2041 ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga
     2101 gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga
     2161 catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa
     2221 tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag
     2281 acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca
     2341 agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa
     2401 acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag
     2461 tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg
     2521 gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca
     2581 gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga
     2641 ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag
     2701 caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt
     2761 gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag
     2821 ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa
     2881 tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg
     2941 agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag
     3001 aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata
     3061 acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca
     3121 acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac
     3181 caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga
     3241 ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga
     3301 tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg
     3361 tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga
     3421 aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga
     3481 atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag
     3541 caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg
     3601 cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta
     3661 tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa
     3721 tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca
     3781 aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag
     3841 tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga
     3901 gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt
     3961 gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg
     4021 aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag
     4081 tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa
     4141 ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta
     4201 ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc
     4261 agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat
     4321 tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt
     4381 tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatc cttggctctt
     4441 ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt
     4501 attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta
     4561 aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt
     4621 attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac
     4681 caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg
     4741 ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata
     4801 tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt
     4861 acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa
     4921 agattttttt acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct
     4981 ataaaattca tcta
//
LOCUS       DAZUMB010000157         4976 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000157 DAZUMB010000000
VERSION     DAZUMB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.162"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..1255)
                     /gene="nagA"
                     /locus_tag="ACG80L_002549"
     CDS             complement(107..1255)
                     /gene="nagA"
                     /locus_tag="ACG80L_002549"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS5024741.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1331..2167)
                     /locus_tag="ACG80L_002550"
     CDS             complement(1331..2167)
                     /locus_tag="ACG80L_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024742.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2351..3568)
                     /locus_tag="ACG80L_002551"
     CDS             complement(2351..3568)
                     /locus_tag="ACG80L_002551"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5024743.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3717..3878)
                     /locus_tag="ACG80L_002552"
     CDS             complement(3717..3878)
                     /locus_tag="ACG80L_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024744.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3951..4034
                     /locus_tag="ACG80L_002553"
     tRNA            3951..4034
                     /locus_tag="ACG80L_002553"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3985..3987,aa:Leu,seq:gag)
     gene            complement(4274..>4976)
                     /locus_tag="ACG80L_002554"
     CDS             complement(4274..>4976)
                     /locus_tag="ACG80L_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024745.1"
                     /translation="PGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNI
                     LFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIW
                     SSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILM
                     IMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGL
                     TVLGICTFIITVINR"
BASE COUNT         1529 a         1025 c          797 g         1625 t
ORIGIN      
        1 aattaaagaa acttttgaaa cttatgtaaa gtgattttta ctagaacaca aaaaaacctt
       61 aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat gcgtggaaac
      121 gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat acgataaagt
      181 ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag ctgattgctg
      241 gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag tctacaacat
      301 ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat tctagacgtg
      361 ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca tcaggcatcc
      421 caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct ttttccatta
      481 ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca gaaaagacat
      541 gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat ccacgcatac
      601 cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc gcttcttcta
      661 gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact tgtttgacaa
      721 actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa ccacctgaag
      781 cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt gggttttgtg
      841 cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg attttagctc
      901 ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa cgttctttgc
      961 ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga ccttcagaca
     1021 tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag ccgtgaatat
     1081 gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg ttgacttctc
     1141 cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca agatagcctg
     1201 catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt ctcattctat
     1261 tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag tcaagaatgg
     1321 tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag ccttcataga
     1381 atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc acaaataaat
     1441 cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga ttcacctgtt
     1501 ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta taaaaaattt
     1561 gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg atgcttcgac
     1621 tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt ccgatacaac
     1681 taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta gagtagctta
     1741 atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg aattcgcaaa
     1801 caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt tcagaaatat
     1861 cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa caactatcca
     1921 acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc atcacatgat
     1981 caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc ggcagttcct
     2041 cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa agcatttttt
     2101 gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac tcgattaacg
     2161 tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat tttactttag
     2221 gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt tatttaagaa
     2281 aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact gaaaaatttg
     2341 gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc ttagctgttt
     2401 gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc tggttccaat
     2461 taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc aaaacgaatt
     2521 tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata taaaaagcag
     2581 cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag atatattctc
     2641 tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga taaccgccta
     2701 gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg gaggacaaca
     2761 tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg accatccagc
     2821 ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta acaacgaaca
     2881 aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact aagcgatcat
     2941 agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg atttcttcta
     3001 ataattcttt tggataaagt gccccagtag gattattagg attaatgata acaatagctt
     3061 ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat tctgcctctt
     3121 catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc gtccacaacg
     3181 gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc tgcatacaca
     3241 tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc acattcggaa
     3301 aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc cccttggaat
     3361 ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact tcgtttggtg
     3421 cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta atgccctctt
     3481 cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg acaccttcta
     3541 atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc aaaaagttag
     3601 aactttccaa attttttgtt atttactatt tcaatatcat actccatcct agaaaatttt
     3661 gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt gagaacttaa
     3721 ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc acaaatgatt
     3781 cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac acctaagatc
     3841 attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc tcccattttt
     3901 gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac gcagatgtgt
     3961 tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg tgggttcgag
     4021 tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt ttttgtttga
     4081 acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga acgtacaatc
     4141 atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt agaaacttgc
     4201 aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac taaagcgagg
     4261 gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata ccaagcacgg
     4321 ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt agctctttaa
     4381 ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc cgcattccgg
     4441 aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc attatcatca
     4501 aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt ccaacaatta
     4561 aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga taaaaaagtg
     4621 acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc caaatcattg
     4681 cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca aaataaataa
     4741 tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt tgacatttcc
     4801 tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc atgtctaaaa
     4861 aaaacggctt tccaaatgat gaaataccta aatctaactt attaagaaag ttgaaatttt
     4921 tatattcagg aaaccattta ttaatttcat gtgcagcacg taatgcattc cctgga
//
LOCUS       DAZUMB010000158         4910 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000158 DAZUMB010000000
VERSION     DAZUMB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.163"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1783)
                     /locus_tag="ACG80L_002555"
     CDS             complement(17..1783)
                     /locus_tag="ACG80L_002555"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024746.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1743..3496)
                     /locus_tag="ACG80L_002556"
                     /pseudo
     CDS             complement(1743..3496)
                     /locus_tag="ACG80L_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3606..4175)
                     /locus_tag="ACG80L_002557"
     CDS             complement(3606..4175)
                     /locus_tag="ACG80L_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS5024747.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4193..>4910)
                     /locus_tag="ACG80L_002558"
     CDS             complement(4193..>4910)
                     /locus_tag="ACG80L_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5024748.1"
                     /translation="ALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDVK
                     VGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTES
                     VRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLF
                     LFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKD
                     IKLRVEDSTLIEQIFNDITK"
BASE COUNT         1630 a          934 c          666 g         1680 t
ORIGIN      
        1 tttaaagcct atttcatcat cgtgattgag cttctttttc atagacgaat ttctcagata
       61 gtttagtttt ctttatgaga tttttgtata tttctgacct tcgatacaac tcattatgag
      121 taccttgatc aactagtttt ccttgatcca tcactacaat tttatttacc ttttcaatag
      181 acttcaagcg atgcgatata attagtactg tcttatcttg tattaaacga tttaaacttt
      241 cctgaatacg tttttcattt tccgcatcta aactggcagt aatttcatct aaaattaaaa
      301 ttggcgcatt tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct cctccagaaa
      361 gtgaacttcc gttttctccg accaatgtat gataaccgtc aggtagtttt tcaataaagt
      421 cttcgcagtt agctaaacga gctgcttttt tcacttcctc atctgacgct tgactatttc
      481 caatacgtat attttccaaa atacttgtat taaacaaagt tacctcttga aaaacaatag
      541 cgattttgct aaacaaagaa gctggagata cacgtttaat atcataccca tcaattaaaa
      601 tacagccttc atcataatca tacagtctag acactaattt caagatacta gttttcccgg
      661 aaccactagc acctactaac gcagtcactt ctccttgttt agccgtaaaa gagatatgat
      721 ctaaaattgg ggtattgtta tcatatgaga aagagacatc tcgcaactca atgtcaaaag
      781 atttcagagg actatctgat ccctcttgga tactggtttc cttcatagct ctaattcgct
      841 gtattttagg agctaaataa aatatttcaa gcactgcttc tttcatagaa tcgaaggaat
      901 cctttatttt tattgcggct agtaagtaac caacgacgta gagtatagtc acttcacccg
      961 ttaacagtaa gtgaacacct actagaatga ctactgctaa actaacatat gagaaaatag
     1021 aggataaggc catgactgaa aaagtactta attctacctt taaatgaatc ctttcactct
     1081 cttccatttt tttgaaaagt ctgtcttgaa cttttttaga tagattaaag ctattaatct
     1141 cttgctgcaa ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat
     1201 atttagtatt ggctttcgtt tgggatttct ttgataacaa gattaagaca aaactaaata
     1261 acgttggcaa aataacagct aatcccattt tgacattacc aaccaaaagc atcactgaaa
     1321 taaaagggaa aaacagagcc ataccgccgg ctttaggtat tgcatgactc atcgcatgct
     1381 caataccttc aacgtcagac atgatagttt gagataaatc tgataaatta tgttttgaaa
     1441 aataggataa tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt
     1501 ctttataagt actattatat aagttttcat actccctatc taacaaccaa tacatgaaaa
     1561 gcaaagtaac aaacgatacg gcaaaataaa gccaacgggg ctttgtattt tctaaaacta
     1621 attcgtctat cagcatcatt ataatgaatg ctggtaccat attaatgcag tatacaaaaa
     1681 aactagaaat agaagcttta gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt
     1741 ttttataaaa acttttcatt gtcaagcctc caatcatttg cattctcata attagtgacc
     1801 aattgtttat acacttgatg tttagccatt aattcattat gatttccacg ttctactatt
     1861 ttcccttctg acaagacaag aatttcatct acagatttaa tgctggataa tcgatgagct
     1921 atcataataa ctgttttttc tttcatcaga tgttgaaaag ccttttgtag ttcatactca
     1981 ttatctggat caatagatgc actggcttca tccataataa caatctttgc atcttttaaa
     2041 attgctctgg caatagctat acgttgtttt tctcctcctg acagataaac tcctttacta
     2101 ccaattaagg tatgctctct ttcgggaaat ttttctaaaa tcgattcgca accggctaat
     2161 cttagcgcgt caaatacttt acttgctggc gcttgaggat ttgctataga aacgttatcg
     2221 taaatactct gattgaatag tttcgtatct tgaaagacaa aagcaattgt gttaataatt
     2281 gtgtcttttg aatagtgatt taaaggctta ccaccaattt taatctgtcc cttatccaca
     2341 ttgtagaaac ctgaaattag tttagctatc gtactctttc cgcttcctga ttttcctact
     2401 aatgcataaa ttcttttttg ttctaaaact aagttgaaat tttctaaaac acactcttct
     2461 ttgtatgaaa agctgacatt ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc
     2521 ccatatttta gttggtcttg cttcatttct aaataaaggg agtctagtgt ttcgactgca
     2581 tatcttgctt caaacaagtg catacttaca tacattattc tcatgaaaga aacaaaaata
     2641 attccactta aaaaaaacaa catcaacaag tcaacagcta atatcttggg agtggtaaga
     2701 gaagttataa aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa aaatagccat
     2761 tgaaaaagaa caaaaggtgt tttcccactc aaagaatact tatatgcaaa cttagcataa
     2821 tcgttaattg ctttattcaa ggctttaaat gatgttactt tagcaccaaa tatctttata
     2881 acttgaattc cgcggatata ttctaccgtt tcagagctta acacatctaa cgattcttga
     2941 tatttttgaa taaaatttgt attctctgtc attttcttta acaagaatac accaattact
     3001 aacatggcga ttagaaccaa tccaatttta aagttaatga aaaagcctaa ggcaactgat
     3061 aaaacaggtg ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt
     3121 gttttagccg cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa
     3181 aaacgaaaac ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag
     3241 cgaaacccaa ggatatgaga aaataagcca gaaaaaatat aaaataaagc tctaaagtta
     3301 accaagcaac agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta
     3361 gagactgaag aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat
     3421 cagaaaataa agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt
     3481 ttttgtatac tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa
     3541 ctgttaatgc tttccgtaat agcgtaaatt agagatgtac aaacagataa aaggtaaact
     3601 acgttctatt ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat
     3661 aaacttgcta caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt
     3721 gcatccattg ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta
     3781 aagaaccata taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac
     3841 gcaacttgag gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc
     3901 gccatactaa tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat
     3961 aacaataaaa caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata
     4021 taaacaaata tgattccaga taataatcca aaaatcccgg gggatatgct gtgaccaaac
     4081 gccccaccaa tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta
     4141 gctaaaccaa taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg
     4201 ttatgtcatt aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt
     4261 tttttattcc accatttttt aaataaataa ttctattgca tgttttataa agaaattccg
     4321 gatcatgagt agctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa
     4381 tcctttccat attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag
     4441 tttttgacaa tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa
     4501 acggtaattg atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat
     4561 ctttactaac tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt
     4621 ggtaatccat ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta
     4681 tctttccttt ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca
     4741 caccgttttt ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt
     4801 ttagaatatt ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga
     4861 tatagggaac ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg
//
LOCUS       DAZUMB010000159         4886 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000159 DAZUMB010000000
VERSION     DAZUMB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.164"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..465)
                     /locus_tag="ACG80L_002559"
     CDS             complement(25..465)
                     /locus_tag="ACG80L_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5024749.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            625..1131
                     /locus_tag="ACG80L_002560"
     CDS             625..1131
                     /locus_tag="ACG80L_002560"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5024750.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1299..3212
                     /locus_tag="ACG80L_002561"
     CDS             1299..3212
                     /locus_tag="ACG80L_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5024751.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3217..4218
                     /locus_tag="ACG80L_002562"
     CDS             3217..4218
                     /locus_tag="ACG80L_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5024752.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4420..4626)
                     /locus_tag="ACG80L_002563"
     CDS             complement(4420..4626)
                     /locus_tag="ACG80L_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS5024753.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1801 a          749 c          994 g         1342 t
ORIGIN      
        1 aaaagcagat tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc
       61 gacaagatga ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag
      121 gaacatcctg attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat
      181 aatcggtttt ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact
      241 gagttcatct attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc
      301 ggtcacgccg ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg
      361 acgaattaag ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg
      421 tcttgaagac gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag
      481 ctaagtattt atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta
      541 aaataagaaa atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca
      601 agaaaaaaga aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat
      661 ttacaaaaag gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca
      721 ttagtgaata aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa
      781 cacgaagatg ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct
      841 attcctgagc acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat
      901 ttgagtgttt tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca
      961 gagtattgta tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg
     1021 tgtagaggag caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct
     1081 ttttacgaga aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct
     1141 tcgtttaaaa aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga
     1201 gaaaataatc ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt
     1261 tgcgtttaca tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac
     1321 aagtattaaa gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga
     1381 cagtatatgg agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa
     1441 ctactgatgc tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa
     1501 caacggttga gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc
     1561 aacaaacaga agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag
     1621 taagtgacaa agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg
     1681 gtaaagaata tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag
     1741 aagacatcaa gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg
     1801 gtgtagataa agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt
     1861 ttgcaaaaaa cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta
     1921 cagaaaacac aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac
     1981 aagatggcaa aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag
     2041 tcatgatcca tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca
     2101 atgattttta taaggccggg tataatttat tatttgttga ttccagagcg actggtaata
     2161 gcggaggcga ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa
     2221 atcaagaagt tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag
     2281 cagctacgat gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg
     2341 aaaattgcgg atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag
     2401 cccccgcact accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg
     2461 aagatttatt tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata
     2521 caaaagctgc tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg
     2581 gaacagcaga tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag
     2641 ggtataaaga tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc
     2701 atgctgccta cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa
     2761 acgtaaagta tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc
     2821 tttacggagc ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat
     2881 tagcaaatgt tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt
     2941 tctattacaa aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag
     3001 acaataaggg aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc
     3061 agatgacagt gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg
     3121 gagaaaagaa acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta
     3181 tcacatggat ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg
     3241 attgcagtac cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc
     3301 acaccaaagc cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa
     3361 gtatatgccc gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa
     3421 atatcctatc cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa
     3481 aagaaaaaat taccgattct tttctggatg catggaggcg catatgtagg aggggataaa
     3541 aatgattgtc gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat
     3601 atagattatg aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa
     3661 gccattcaag cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt
     3721 ggtggaaatt cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa
     3781 gaaattaaaa aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt
     3841 atttctttgt ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct
     3901 agctttcttt atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca
     3961 gagaaggtca aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc
     4021 ttaacggatg gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt
     4081 gagaaagcgg gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac
     4141 catgaatatc agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta
     4201 atgtttttca aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc
     4261 caagtcacaa ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt
     4321 gtgactgagg agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca
     4381 cgattattgt cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg
     4441 tttaaaaaaa gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg
     4501 taagcaaaag aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca
     4561 aagatgaata gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc
     4621 tttcataagc agcacctcct tttttataat tgtatgataa atagattaag aatctataaa
     4681 tgtgacaagt ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg
     4741 gctaatgata agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg
     4801 tctagaccaa taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa
     4861 gaatttcccc aaaaattcga tgtatc
//
LOCUS       DAZUMB010000160         4824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000160 DAZUMB010000000
VERSION     DAZUMB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.165"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1442)
                     /locus_tag="ACG80L_002564"
     CDS             complement(<1..1442)
                     /locus_tag="ACG80L_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFS5024754.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIYDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRL
                     TDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATW
                     LWKGKEPLFFCSKIRNEVDKKLFQARVTSL"
     gene            complement(1414..2613)
                     /locus_tag="ACG80L_002565"
     CDS             complement(1414..2613)
                     /locus_tag="ACG80L_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5024755.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(2743..4119)
                     /locus_tag="ACG80L_002566"
     CDS             complement(2743..4119)
                     /locus_tag="ACG80L_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5024756.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(4119..4775)
                     /locus_tag="ACG80L_002567"
     CDS             complement(4119..4775)
                     /locus_tag="ACG80L_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5024757.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT         1434 a          933 c          723 g         1734 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat gactcgttga tgatttttac ttatggtaca ttggtttagc
      361 agaggacagt tcacacattc ttttggattg gatttgtatt ctcggtaccc ttctcgagta
      421 accgttgaaa aagcgagcat ttgatcttgc ggacaaatat aacaatcaaa atactcgtca
      481 tagagaaact cattttttga taatagcccc tttttccctt tgggccttga ataaggcaaa
      541 caaggtcgaa ggttcaaatg agtcaaaaag tgaacaaact taggtgtctt atacccagca
      601 tcagcgacaa cacaattgat ttcaggaaaa agtctcttcg attgtttgac gagctgaaag
      661 gcgacttgac tgtcataaat atttccagga gtgatgattg tgctaagaat aaatccattg
      721 tcgtcgcatg aagtatgggc agaataagca aattgttttt ctcgttcgcc tttgacgtag
      781 tagccgcttt ccggatcagc tttacttatt tttctttctt tcagcttcac ttccttttct
      841 aagtccaaag taaaagggcg ttttccagcc tctattcgtt gacgattgat ctcatcttct
      901 aattcatcct gataggcttt cgcttcatca tgggtcattt ctttcgtaaa cttatgttta
      961 ttggcgttag ctttgatgtg ggtagaatcc agatacaaat tatcctctgt tacaaaacct
     1021 gctttaactg cttgttctaa aatataggca aaaatatcct caaacaccgt cgtttctcta
     1081 aatcggcgaa cataattctt tccaaaagtt gagaaatggg ggattttctc ctcgaaggag
     1141 tagcctaaga accagcggta agcgacattg gtatcgactt ccttaatggt ttgtcgcatg
     1201 gaacggatac cgaataaata ctgaataaac accaatttga tcagaatcac tggatcgata
     1261 cttgggcgac ctaaatcgga gtaagtggct tcgacgattg gataaataaa ttcaaaatca
     1321 agcgcggcat caatctttcg aacgagatga tcttgaggaa ctagatcatc aatcgtaagc
     1381 atcgcaaatt gatttctacc attcgtattg tttttagaca tcatagctat cactccttgc
     1441 attcgttttc ataaatctat tataaaagaa aagactgtag acatcatcca taaaatggac
     1501 tttgtctaca gtctgcgcat tctcattaga gaacactccc ttttttgtga cttttctgaa
     1561 gctagagtga atactttcaa ctgcattagt agtgtacatg atttttctaa tggcgctgcc
     1621 ataatcaaag agttgttcaa catgagaaaa gtttcgtttg catacatcta ctgcaccaga
     1681 ataatgagac caccgatttt gaaaactgtc gaaggcagcg tgtgcagcgt ttaaagaaga
     1741 agcactgtag aattttttca tgtctcgaca gacttcctta taatccttac ttgggaggta
     1801 tcgtaacgca tttcgaacaa gatgaacaat acagcgctgc acaatgacag aaggaaaaat
     1861 tgcttttgcg ccttctttga gaccggaaac accgtccatc gaaatgaaaa aaacatcttc
     1921 tacaccacct actttcagtt catcaaatac ttgcatccag cgatttttag attctgtttg
     1981 attgagccat aggcctaaaa tctccttgtt tcctttaaga tcatagccaa gaatggtgta
     2041 cacagcatat tctttagctt catagttttc gcgtaaagtg acgtacatac aatcaacgaa
     2101 tagaaatgca taacacttgg ctaatggccg agcttgccat tcttccaatt caggaaggac
     2161 ggcatccgtg atatcagaaa tcatttcgtg ggaaatatca aagccgtaaa tatcttcaac
     2221 ggttgcggcg atatcgcatt gactcattcc tcgtgcatac atggaaagaa ccttaccttc
     2281 gatgtcggag acatctcgtt ttctattagg aatcaactca ggttcaaaag aagcctcccg
     2341 gtctctggga acatcaatgt ttactttacc aaagctcgtt ttaagcgtct tattgccata
     2401 accgtttcgt cgattgtcat gtttctttgg ctctttagaa tgggcgtcat accctaaatg
     2461 gttattcaat tctccttgaa gcattttttc aaataaggga ccaaacacat cttttaaaac
     2521 atcttgcata tcctctacag attcgggttg ataagtattc aaaatggatt cagctaactt
     2581 ctcagcatca ggatttcttt tctttctagc catcgtgtaa tcacctcttg atcttatttt
     2641 agaaaaataa aaaccgtgaa gcaaccaccc gcctaggatt gatggttact tacacggttt
     2701 atattacact ctcatttttg gtgctaaaaa gcaccgaaaa ttttaattat tgtcaattaa
     2761 taattctttt ggattccttc ttaatatatt gaatgaagaa ataatcaatg atacaagaag
     2821 tactaaactc ataaatataa ctacataaat catgaattta ggtaatacat tgatatctaa
     2881 acttgataat gtcttgttaa atccttcagc ttctgcccca ccacctaatt ggctagatgc
     2941 agattgttta gctatttgtt tagctatatc accagtaacc ttattcaata tattattccc
     3001 aagcttgtca gctgtatatt gagctaagaa ataggaacca acgaatgcgg ggattgatat
     3061 aaataccatc tcaattagga attgaccaaa aatttctaat tttgatatac ctaatgatag
     3121 taatactgct atttctttct ttctagcatt catccataag aataataata atgaaactac
     3181 aactcctgca aatattaatg agccaaaaaa taatttattt gaaattgagt aaatacctga
     3241 tatagattgt tgtaatgcag ggtaatttga tgaactttta atcaaattat attctctcca
     3301 atttatatct aatttcccaa gatcttttat tacactatca agattcttat caccttttac
     3361 aaagaatgtt gcatcttggt atacagctgt atcttcggta ttaccataaa ctttagcagc
     3421 tgtatgaata tcagtgatta atgtattttc gtatagttct tgtgctgcac ttactccacc
     3481 gctattatgc ccatcgaata gacctttaat ttctacttct actgtttcat ctgcaccttt
     3541 ctcattatca gcatcaaata aattagattt tatttttatc ttatctccaa ctttaaggtt
     3601 atttttttca gctaaatctt tatgcattaa gattttattt ttatcttcat tttctaaatg
     3661 ctttccttct actaatttat atgcttctga tacaaatttt gtttcttttg ttgagtcgtt
     3721 aaccccagtt aacataactg ttcttttaaa attctttgct ctttcaggtg attgattcgc
     3781 aagagtctct tgagtttcaa taatatcata atcaactaaa tctgcaacac tgtttattct
     3841 ttttacatag gagtctatac tatctgtttg agatatcttt ttaatatctt caccttttac
     3901 atttccccca cctctaggtg ttcctggatt tacttgtcta tttatctcca tagaaaaact
     3961 atttgttata ttagcaaatg tttcttttga agctctgtcc gtagcatctt taatagataa
     4021 actaatgaga cttaaagttg acatagataa aataactaat atgataatta atgattttaa
     4081 actttttcta gttacataag caaatgcatt ttttatcatt attcaacctc caaattcatt
     4141 ttatttactt ttttcaactt tttaccactt agttctaaaa tgatatccgc agaatctgct
     4201 acttccttac tatgtgttac aactattaca catttatttc tatctttagc taatgtcttt
     4261 aatatattaa ttatttctcc agcagtaaca ctgtctagat taccagtagg ctcatcagct
     4321 agtattattg gagcatctga taccaatgct ctagcaatag ctactctttg ttgctgtcca
     4381 ccagataatt tcataacatt tctttttatt tgttttttat ctaaacctag ttcaaacaag
     4441 atactttcat ctgctgattt atttactaat ctaatatttt caattggtga taaataatct
     4501 attaaattat agttttgaaa tactaaagat atattatttt ttctatgatt actatatcct
     4561 ttattttgta tatcttcatt cttaaacaat atttttcctg tttgaggttt atcaagtcca
     4621 gcaagtaagg aaagaagtgt agattttcct gttcctgact ttcctactat cgcataaaac
     4681 ttcccaagtt caaatttttg atttactcct gacaaaactt tttctttaga atttgcataa
     4741 ctatatgcta cattctttat ttctaatata tccattattt tctcctaact tatttttgat
     4801 aagatttctt taggcttctt gatt
//
LOCUS       DAZUMB010000161         4788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000161 DAZUMB010000000
VERSION     DAZUMB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.166"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACG80L_002568"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACG80L_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS5024758.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACG80L_002569"
     CDS             1019..1183
                     /locus_tag="ACG80L_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024759.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACG80L_002570"
     CDS             2141..3118
                     /locus_tag="ACG80L_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5024760.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACG80L_002571"
     CDS             3128..3625
                     /locus_tag="ACG80L_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5024761.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACG80L_002572"
     CDS             4001..4282
                     /locus_tag="ACG80L_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024762.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACG80L_002573"
     CDS             4284..4700
                     /locus_tag="ACG80L_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024763.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAZUMB010000162         4719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000162 DAZUMB010000000
VERSION     DAZUMB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.167"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1118
                     /locus_tag="ACG80L_002574"
     CDS             72..1118
                     /locus_tag="ACG80L_002574"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5024764.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="ACG80L_002575"
     CDS             1135..2358
                     /locus_tag="ACG80L_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5024765.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="ACG80L_002576"
     CDS             2447..3334
                     /locus_tag="ACG80L_002576"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5024766.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="ACG80L_002577"
     CDS             3420..3650
                     /locus_tag="ACG80L_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS5024767.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="ACG80L_002578"
     CDS             complement(3707..4672)
                     /locus_tag="ACG80L_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5024768.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1594 a          787 c          960 g         1378 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttga
//
LOCUS       DAZUMB010000163         4713 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000163 DAZUMB010000000
VERSION     DAZUMB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.168"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..359
                     /locus_tag="ACG80L_002579"
                     /pseudo
     CDS             108..359
                     /locus_tag="ACG80L_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="ACG80L_002580"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="ACG80L_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS5024769.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1739
                     /locus_tag="ACG80L_002581"
                     /pseudo
     CDS             1656..1739
                     /locus_tag="ACG80L_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1788..1871
                     /locus_tag="ACG80L_002582"
     CDS             1788..1871
                     /locus_tag="ACG80L_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFS5024770.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="ACG80L_002583"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="ACG80L_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS5024771.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="ACG80L_002584"
     CDS             complement(2996..3859)
                     /locus_tag="ACG80L_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5024772.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="ACG80L_002585"
     CDS             complement(3861..4133)
                     /locus_tag="ACG80L_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5024773.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4365)
                     /locus_tag="ACG80L_002586"
     CDS             complement(4150..4365)
                     /locus_tag="ACG80L_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS5024774.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..>4713)
                     /locus_tag="ACG80L_002587"
     CDS             complement(4457..>4713)
                     /locus_tag="ACG80L_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFS5024775.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1447 a          796 c          865 g         1605 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctt gataatattt tggaaaacca
     4621 agttatcttc tttatgttgc ttatacagtt cttccaggtt tgattttatc gttgcgctgt
     4681 ccgtatcaac taaataagga acaaaaccga tca
//
LOCUS       DAZUMB010000164         4609 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000164 DAZUMB010000000
VERSION     DAZUMB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.169"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..398)
                     /locus_tag="ACG80L_002588"
     CDS             complement(84..398)
                     /locus_tag="ACG80L_002588"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5024776.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(449..703)
                     /locus_tag="ACG80L_002589"
     CDS             complement(449..703)
                     /locus_tag="ACG80L_002589"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5024777.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(896..1171)
                     /gene="prgO"
                     /locus_tag="ACG80L_002590"
     CDS             complement(896..1171)
                     /gene="prgO"
                     /locus_tag="ACG80L_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS5024778.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1143..2096)
                     /locus_tag="ACG80L_002591"
     CDS             complement(1143..2096)
                     /locus_tag="ACG80L_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5024779.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2708..4201
                     /locus_tag="ACG80L_002592"
     CDS             2708..4201
                     /locus_tag="ACG80L_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS5024780.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4336..>4609
                     /locus_tag="ACG80L_002593"
     CDS             4336..>4609
                     /locus_tag="ACG80L_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS5024781.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1541 t
ORIGIN      
        1 gcttgaattc ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta
       61 tgttttcttc tagtctacac accttaattt ttctgtctag ttaattgtag cctatccatg
      121 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
      181 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      241 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      301 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      361 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      421 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      481 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      541 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      601 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      661 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      721 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      781 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      841 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      901 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      961 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
     1021 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
     1081 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1141 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1201 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1261 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1321 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1381 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1441 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1501 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1561 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1621 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1681 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1741 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1801 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1861 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1921 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1981 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     2041 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     2101 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2161 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2221 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2281 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2341 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2401 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2461 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2521 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2581 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2641 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2701 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2761 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2821 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2881 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2941 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     3001 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     3061 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3121 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3181 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3241 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3301 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3361 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3421 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3481 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3541 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3601 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3661 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3721 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3781 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3841 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3901 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3961 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     4021 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     4081 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4141 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4201 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4261 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4321 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4381 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4441 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4501 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4561 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaa
//
LOCUS       DAZUMB010000165         4605 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000165 DAZUMB010000000
VERSION     DAZUMB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.170"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            276..872
                     /locus_tag="ACG80L_002594"
     CDS             276..872
                     /locus_tag="ACG80L_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5024782.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(930..1412)
                     /locus_tag="ACG80L_002595"
     CDS             complement(930..1412)
                     /locus_tag="ACG80L_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024783.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1518..1715)
                     /locus_tag="ACG80L_002596"
     CDS             complement(1518..1715)
                     /locus_tag="ACG80L_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024784.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2010..2768
                     /locus_tag="ACG80L_002597"
     CDS             2010..2768
                     /locus_tag="ACG80L_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5024785.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2828..3256
                     /locus_tag="ACG80L_002598"
     CDS             2828..3256
                     /locus_tag="ACG80L_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024786.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3302..3374
                     /locus_tag="ACG80L_002599"
     tRNA            3302..3374
                     /locus_tag="ACG80L_002599"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3335..3337,aa:Gln,seq:ctg)
     gene            complement(3524..3895)
                     /locus_tag="ACG80L_002600"
     CDS             complement(3524..3895)
                     /locus_tag="ACG80L_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5024787.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4110..4295
                     /locus_tag="ACG80L_002601"
     CDS             4110..4295
                     /locus_tag="ACG80L_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024788.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4378..4566)
                     /locus_tag="ACG80L_002602"
     CDS             complement(4378..4566)
                     /locus_tag="ACG80L_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024789.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT         1545 a          879 c          727 g         1454 t
ORIGIN      
        1 aatttttaat ggcaatatca taaaaaaaag aagatagaga tttaataatt atatatcagc
       61 tgtttttttc aaataagcaa aaattcctaa aacacaacaa tgaaagcgat aactaagaaa
      121 atccttacat aacgattttt cttattaaaa ttgttttttg aacttcgctt attttttgtg
      181 agcatatctg tttacttact ttttgtaagg tgctatactt catttcgtaa tcaattaaaa
      241 aaatgcaaaa cgcaaatcaa aggagaaatt ttattatgac aacttttact gacactttga
      301 aaaaccgccg ttcaatctat catctaggac gtaatgtttc actttctaat gaagaattaa
      361 caactttgat caaagaagca attaaagaaa gcccaactgc tttcaatgct caatcaacac
      421 gcgcagttat ccttttcgga gatgcacatg aaaaactttg ggaaatcact gaagaagcac
      481 ttcgcccatt gactccagca gaagctttcc caaatacaca aaacaaatta gctggtttca
      541 aaaatggtta tggtacagta ttattcttca aagatactga cgtggtaaaa ggtctacaag
      601 aacaatttga attatatgca gacaacttcc cagattggtc agaacaatca aacggaatcg
      661 caacagctaa tacttgggta gcattagttg ataaaggatt gggcgcaaac ttgcaacatt
      721 acaacccagt tattgacgaa gcagttgcaa aagaatggaa tatcccttca aactggaaac
      781 tacgttcgca attagtattc ggttcaccag aaacaccggc tggggaaaaa gaatacatga
      841 acgatgccga tcgcttccgc gtatttggat aattttattt gaaataaaaa acagaggttg
      901 tgacaaacat cttgtcgcaa cctctgtttt tattctggat caatcataat atcaatctct
      961 gcctcatcgt ttaagcgttg ggacaaaaat ggaactaatt cttctaatga tgcagaaaat
     1021 ttcagcatcg gtcctcgcca tccgtttcgg aaaaaattca tttttacgat tttagaacct
     1081 tttggctgat ataatacaat tttattgatc tcagaaaaag gaacaccttt tttgtcaaag
     1141 ctattcaaaa caagtccttc ttctgtgatt cctctactgt ccaacaaaaa actaataaaa
     1201 atcattgctg acaagatccc ttttgccaat tcatctgctg taggatgact gatgagtgta
     1261 taccaaatga ctagtgacac taagaagggt atcaccaagt agacccatcg atttcgcaca
     1321 gtccgataaa taatttcttt tcgaaccagc caaagaaata aaaacatcac aacaacgaat
     1381 aaaaacaata gaaccgtaaa gaatgttacc atgacaactc gcctcgcttt ttcttctcca
     1441 gaaaatagga aaagaatact gatttacctt ctcaaagaaa ttggacttgc agcacgccgc
     1501 acgtccaatt gttttcttta ttcaaatgta tcatattttg caacaaccat gcttgctttt
     1561 tcatctaata attcttggtc ctctgcagca cattgggcaa ctttgaccac ggcgcagttt
     1621 gtcatttttg atacaacttc accaatgacc gtttttgtaa aaccaattgg tttgcatgca
     1681 tattgttctc ctaaattaat catttcttcc gtcatcaaaa tcctcctaaa ctgctggaac
     1741 ttttctccac attaccactg ctatttcttt ggagatttaa ctcattgatt tttatttaca
     1801 gttaaaaatc aaccttacag gcattatagc gtactgtttt cgttttcaca attaaaacag
     1861 ctgttgtatt tagaagaatt tttttcaaaa aattaaaaca tattccttaa aaactaatta
     1921 tttcctacat attaaaatat aatacttcca tttccaataa aaatacattt ataataggaa
     1981 agataaacag ctgaaaaggg agatttcaaa tgaagaaaaa aacgttcaaa aaactattgt
     2041 ttctaggggg cacagctgct cttttattaa gcggtacaag ttattaccta catatccatt
     2101 ctccagaaca tacaatgaac actgcacaaa aaagttcaac acaaaaaacg aaaaagacaa
     2161 aagagaaagc agaagtttta ctagatgttc cattagagag ccagtttgat tctccttctt
     2221 tagaaaatgg ctgtgaagtc acttcgcttt ctatgctttt atctttttat ggttatgaaa
     2281 caactaaaaa ccagttagct gagcaattgg attacgtccc tgttttcaat gcagatggga
     2341 ctcatggcga tcctaatgaa ggatttgttg gtgatatcag tggcggagac tgggctatgg
     2401 tcgtctatgt tcccccagtt gcctcccttg ctcagcaaat cgtccaaacg gattatcaca
     2461 cttttccaaa aaccgatgct caactcgatg atatcaaaaa agcactatct aaaggaaatc
     2521 ctgtctggac ttctgtgact atcaattttg aagtcccaga tgaaactgat tttatgactt
     2581 ggaccacaaa taatggagaa gtaagagtca ctcctcttgt tcatgcttgt gtagtcactg
     2641 gatatgacaa agaaaatatt tatgtcaacg atccctatgg tgtaaaaaat cgagcagtac
     2701 cgatcaacga tttttcggcg attttcactg caatgggtgg acagatgcta actttggaga
     2761 aaagctagta attcaaacaa aaatccctta tactcaagat accaaacctt tgaagggagc
     2821 gtagataatg gaaaaattca cacataaaaa aatggatcca aatgaaattc cgattatttt
     2881 tgtccgtgac tgcaagggaa atgttcaagg taaagtctct attaatgagt ggaacgagcg
     2941 tcgcagacct gctaccctca atgaactgga aatcaaattg tatcgtcaat ctcttgttta
     3001 ttacgcagat caagagtacg agaaagccac cgatttattg aaatttctaa ttgctcgaac
     3061 agagtatact cgctttgaat atattgaacg tttagcaaac atctatcata ttatgaatga
     3121 acctgtcaaa gaatatcagt tattggacac cgtcttgtca gtagcagaac tgattgccct
     3181 tcctgctgga ttagaaaaga aactagtacg ccgcctttta cgagtaaagc aacaactatc
     3241 cgatcaagaa aaataacttg cgttttttta ggaaagtgct atgattattt tgttgaataa
     3301 atgggatgta gccaaactgg taaggcactt gactctgaat caagcaattg taggttcgaa
     3361 ccctgccatc ccaatttgat gtgcttgctg aaaaaatgaa ccccctgtaa aagaacaatc
     3421 tttttgcagg gggattattg tattattttt actacttgta tttagtttac ataattaaat
     3481 tatgaataat aaaatcgggc gtattcttaa tacaaaaact gatttaccca ataattgcta
     3541 atgctttctc cggaactttt tctctaggaa cttcctcata cgtttctatg tgtgctcctt
     3601 tattgctaat tttcaaataa tgattttctt ttaacacaga aagaccatta aattcaaccg
     3661 ttcgttcttt cccattggca tcagcagctt tctgcacata ccttgctgta ccatattcat
     3721 ttacttcctc tggtttttgt gtctttacat agacctctcc tttttcaact aatggattta
     3781 gctgatcaag tataccaggg atttctccat aacttccttc agtataatat ttcagtccaa
     3841 atagcaaaat accactaatc gccgccagtc caatcaaata tttaatcact ttcatctttc
     3901 tacctccttg ttatatattc agtatatggg tcagttaaag aaattccatc cgtcccaagt
     3961 tggagatttt tctcctactt tagaagcgag aatcgagtgt ttttttagta aaaatgatcg
     4021 ttatttgata ttattgacgt gtggataaat cagtttttct cgttttgtcc atgtaaaaaa
     4081 tcatgttgat agcaaaggat gtgttcaaaa tgaatcaaga agaattattt caaaaagtca
     4141 aggaaatgat taagaacggc aatttcgatg gcgcaaaacg atttatcgaa gaacataaag
     4201 aacaattagg accttataaa gagaaagccc aaaatctttt aaaagatgta aatatcgaca
     4261 gtgtcaaaaa caaatttaaa aatctattca aataagtaaa actttcactt ttgtaaaatc
     4321 aaaggaggac aatgctgctg catcgtcctc ctttggttct tttctgaata cttcatacta
     4381 aaataaacgt gtcggatcgt aatgataagt cacattgccg ttcagtgagg cttttcccac
     4441 cacatattta cgattctcca acttcagcca cgcttctcga aaagccatta attcttctct
     4501 ttttacatca atcttttcga tttttccatc gatcaaatct tgaataagtt gttcatatgg
     4561 attcattttt ttcctccatc tgctgtcaat caataagttg taaaa
//
LOCUS       DAZUMB010000166         4523 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000166 DAZUMB010000000
VERSION     DAZUMB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.171"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..769)
                     /locus_tag="ACG80L_002603"
     CDS             complement(266..769)
                     /locus_tag="ACG80L_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5024790.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1128..2153
                     /locus_tag="ACG80L_002604"
     CDS             1128..2153
                     /locus_tag="ACG80L_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5024791.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2476..3354
                     /locus_tag="ACG80L_002605"
     CDS             2476..3354
                     /locus_tag="ACG80L_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS5024792.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3830..3946
                     /locus_tag="ACG80L_002606"
     CDS             3830..3946
                     /locus_tag="ACG80L_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5024793.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1489 a          728 c          822 g         1484 t
ORIGIN      
        1 ggtataaatt aagacggaat tcctttctag attgagatat tgactaggaa gaatgttaag
       61 attttaagcc tttaagctaa aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt
      121 cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt agattagaga tgaaaataga
      181 tttttttaaa ccaatgactg aataaaaaga ccgtcataga tacacattgg aatgtgtcta
      241 ttacggtctt tttttacgaa cgattttatt gaagacggac agttcgggtc aaaaataaaa
      301 aagctacgat cagaaataag aataaaatgt atcccaaaat aatggctaga cggtctaact
      361 tatttgtcga tagatgtttg tttagaagtt gataggccca taaacctatc agtcctcctg
      421 ttgtattagt tatcaaatca gtgatgtccg ctacgccaat accgaatagg aattgaatca
      481 attctgccaa gaagcttaca aggaaaataa tccctaattt tttagaaaaa gaaagtctct
      541 tgaaattgac attcaaaagc accccaaaag gaaaaaagac aatcaagtta taagtggtct
      601 cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc
      661 tgtaagaata agtgagaaat tgtggaagat taaatgatag cttgaataaa atcaaccata
      721 atagaaagaa taaataaatc cctaataaaa attttgatac ataccgcata gacatctcct
      781 ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa
      841 ggaccaacgt agttatcgta cgaaaattag aagctttttt ttgttgggaa tccggtttct
      901 ttaacaaagc taagatagcg agttgcttgg aaaagttgct atcttttttc tgtcaaaacg
      961 actcggaatg ttcgtttttt gttctaattt ctccaagttg tttacttttt gatactaagt
     1021 ttatgaaaaa tcaagaaata gttgttatac taggtaatgc tgacaaacgg gattggcatt
     1081 tcatcttggt tcagcttttt ttccaattac taggaaggag ggatagattg ttcaagactt
     1141 attttcgttt caataaaata caagataatc cttttcgtat catgaatctg atgctactta
     1201 tctttattgt attatggtta ggttacataa atcatgattt tttgatttca agtttttcat
     1261 cattagggat ctttactttt ttctattatc aaaacatacc gatgaaatat ttgctgaaac
     1321 gtatgtttct gatcggttgc ggactgttgg ttgctttcgt tttggggatc ttatcgactt
     1381 atgttttatg gcttgaacca tttgccgtag ctttggttgc tttttccagc agattcgtgt
     1441 tgcgattatt ccatatttct aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg
     1501 gaacgagcat gcagctgcca atcgctcaat taccgatcgt ctcactttat ttttttatgg
     1561 gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt actggattca cggcctgagc
     1621 agacaattga aaaagcaact ttaaaagaaa gatttcacga agagccttta gtgatcattg
     1681 attctgtttt ctacagcgct gccttatttc tttccgttta tgtcagtcac ggcttgaatc
     1741 tgcataatcc ttattggttg accttgtcat gtgcatcgat tttgctagca gaaaacttgg
     1801 atgcgatgaa gcatcgacaa gtccaatatc tgattggatc gatgggtggg ttatgtgtgt
     1861 cggcattctt gtcatttgtg ccgtttacgc agttgcagac catttttctg atcacttttc
     1921 tatatggaat cgcacaattt ctagttgcta gaaattatgc agttgctaat atttttctta
     1981 atccgatggc gttgatgcta tctacactta ttcgtggagc atacttgatt tctttgatcg
     2041 aatatcgttt tttagggatc gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa
     2101 tgacggttgg tttgcaacat tatttaacgg ttgttcgtca aaaattagaa tagaaggaca
     2161 aatatatgtg ccaaaagagg ttatgacaag atttttgtca caacctctaa ttcaaggtgt
     2221 tcaaaagcca gattctcagc attaagtcaa attcacacaa aaatgtggaa aggaactttc
     2281 aaaaaaattt tatatttatt ctatcaaaaa attttgttta tgatggtaac tgaataaact
     2341 ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga tattctttct tcatagaaag
     2401 tttaagtttt atagttagtg tacaataaaa gtatgtttat accatatggt gtccggataa
     2461 tcagtcattt aaacaatgag gttcaaagat atgaaaaggg gaaatttgat gaagaaaata
     2521 agcaatgaaa tccaaattct ggcagaaact ttaactcttc ccaaagtcgt tatagagcaa
     2581 atgattgtac aagcaaaaaa catccttgat gaggtgtttg agcaatatag tcatgaacta
     2641 atggatccta ataaagcaaa ggcgactgca aagaaaatag acaaagtcta tgaagaggaa
     2701 ccttttgcag cattagctat ttatctatat gcagcttctc aaagttggaa aaaaatttat
     2761 caaccattga agattccaag aactatctat ttggcaacaa tgaatgcttt tactagattt
     2821 attcatgaac atttccaagt aacaggaagc tatcgctttg accgtggttt ttggatttgg
     2881 cattacacta gtggcttgat ctttcggatt ggtgaattgg agtacgaaat gacatatttt
     2941 ccgaaagggt acacggatct aagattagaa ggaaagcagt gcctttctct tcatattcct
     3001 tccgatgcag atttatctca gaataagata agagatagtt acgaaagtgc cattcgtttt
     3061 tttgaacttt attttcctga ttaccattac caagtgatgt acacagatac ttggttacta
     3121 tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa tctgtctttt tgcagccgat
     3181 tatcgattat tatcagttga tgaacaggac gatagtggag tcccttggat ctttggacga
     3241 gtagatgccc aaatacatga ttatccagaa agtacgagtt tacagcgaca agcaaaagag
     3301 caattgcttg caggcgagca cattggctct ggcttaggta ttcttacttc ataagtttag
     3361 tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     3421 ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc tgaaattcac
     3481 aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg
     3541 tcccagcctc ttttttagtg ttctatcgaa cacacagtac gttcaatagg taaactagct
     3601 ataacaatag atattaaagt taacggacaa gagggccttt tttaggtagc taaaacgtaa
     3661 taatgaaaat ttatttccag agataggagt caatcagcta gtctgatcga ctgttttaac
     3721 aagttgactt tcattttaac ttttgatacc atttaaaaca caaatcgtaa ttgtttcgat
     3781 ttgtgttttt tagcatgact acaatcaaag aagaaagtgg gtcgtccata tggaaaaaac
     3841 agatatctca agtgcatacc gccgcttgaa aagcccaaat atcaaaacac gaaaacgtgc
     3901 gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa aggtaagaag aaggaaactt
     3961 aattaaaaaa atagccgaaa aacgtttttc ggctattttc aataatcggt ttttgtgtaa
     4021 ggtttccgaa agaactgctg ttgattttcc atttttaaca tatgataaat tggtagaatc
     4081 tttcttttca aattgatacc attccctaat tccagttata tagttaacta cagaaccaca
     4141 aagcacgtac gtttataaaa gtattatctg tagttatttt tcaattccct ttcaacctct
     4201 tattttgacc agtagtattg atcactttac cttttaacac ctttgttcgt ttttgaaaca
     4261 tttttagtca agaatggaaa cgtaactaca taatcaaagg ttttgcgaaa tatttcatga
     4321 aatatttcat gtagaagcga gcaatacggt tgcaaataac cattatcgtt taataagcag
     4381 ttacgaaagc tcccaatatc tctaacgttc agctcactcc atttcttttc ttcaaaaaaa
     4441 tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa ctttaaaaag taacgccgaa
     4501 aaaatatgca aaaaaataaa aaa
//
LOCUS       DAZUMB010000167         4486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000167 DAZUMB010000000
VERSION     DAZUMB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.172"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..294)
                     /locus_tag="ACG80L_002607"
     CDS             complement(<1..294)
                     /locus_tag="ACG80L_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024794.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYG"
     gene            complement(468..1193)
                     /locus_tag="ACG80L_002608"
     CDS             complement(468..1193)
                     /locus_tag="ACG80L_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS5024795.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1480..1722
                     /locus_tag="ACG80L_002609"
     CDS             1480..1722
                     /locus_tag="ACG80L_002609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024796.1"
                     /translation="MLNKNQRDVENIAIFLIKITSSISIIIPMTVTFSKEQFNVAATY
                     LSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(1728..1910)
                     /locus_tag="ACG80L_002610"
     CDS             complement(1728..1910)
                     /locus_tag="ACG80L_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024797.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(1910..2209)
                     /locus_tag="ACG80L_002611"
     CDS             complement(1910..2209)
                     /locus_tag="ACG80L_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024798.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2314..2517)
                     /locus_tag="ACG80L_002612"
     CDS             complement(2314..2517)
                     /locus_tag="ACG80L_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024799.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2532..3278)
                     /locus_tag="ACG80L_002613"
     CDS             complement(2532..3278)
                     /locus_tag="ACG80L_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS5024800.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3356..3664
                     /locus_tag="ACG80L_002614"
     CDS             3356..3664
                     /locus_tag="ACG80L_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024801.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3635..3892)
                     /locus_tag="ACG80L_002615"
     CDS             complement(3635..3892)
                     /locus_tag="ACG80L_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFS5024802.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4190..4330)
                     /locus_tag="ACG80L_002616"
     CDS             complement(4190..4330)
                     /locus_tag="ACG80L_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024803.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
BASE COUNT         1230 a          865 c          711 g         1680 t
ORIGIN      
        1 tccgtaataa gagatatcaa tcaagttatc tctgttgtac tcttctagcg catcaatcaa
       61 gccatcttcg atgacataga tgtattcagg tttatttgaa tgttttgata gatggataag
      121 gtaaacatga tcccaaatac tcacaaaatt tcccaagtca tcttgatcac atgatagttc
      181 ttcgttcgtc aaaagatttc gtctgatttt tcgattattt gtttccttga tattcgactt
      241 gccccattca gaatcagtca aatattgatc taaagtggaa agttctttat tcatatgcta
      301 aaattctcct tagatatgtt ttgtgactct atgcttgccg gctgagtcac ttttttattt
      361 gttgccatgc tttttgcttt tcgatatgtt gttggcttag gatgtttggt ttattgtgtc
      421 tccaccagcg attagcaatt accgtcccta ttcttagcgc ttcagctcta ttcattttca
      481 tcaccgaaaa gtctttgttg tctgttcagt tgatcgattt ccatgcggat cgcagtttct
      541 ggtaaccaca tttcaataaa tgaaacagca tcatcgaatc tcttacgagg taactcgcca
      601 tatcttggga ttgaaaaggt acgtttaaat tcagaccaaa attttgagaa tacttttttg
      661 ctgatttctt cataagctcg gctttctttt ccccctagaa cttccataac tttcatattt
      721 cctttttgct taatttcaaa ctcttgttgt ccgctaattc gcatagtatc tttaagcatg
      781 gaaacatctt ttttaacatc tttcatttct tctaattgat agatcatcat gtcttcaatt
      841 gtttgaggaa cagtattctt ccgaataaca tcttccattt cgttgaaagc ttcaataaat
      901 tttagtttga aatttattgc tttacttcca gtgaatccca tagctagcaa ggaaaatccg
      961 tctctattca tgaaataaac tctccgactt cttccgtatg agtctggctc gttcccttcc
     1021 acaaacatct gtccaaaatt ggacccatct tcaacattgg ccgaattttc gaccgatctt
     1081 tttattgatt caattgcttc tagtacatgc ttatgtttct ttttgaaact ttctgccact
     1141 tgtaagctcg tagttacagc ttctttattt ttcaaaatta ctaattcttg cattatttct
     1201 tctctccttt ttggtataat tttcgttaga aagtgaggtg attctatgag gaaaaagctc
     1261 aattacattc ttactgcgac tttaaccata tatataattc tgtttattac cttatctttc
     1321 ctgtcttcaa ttaaaaaaat tccttttatt acattgaata aaaatagtat tttgaaggag
     1381 ttcaataaca ctgactacat cgcatttcta aaactttttt cagtctgtgt gctaatatca
     1441 tgtgtaatta tttttattgt tgctaaaaca tgttggacaa tgttaaataa aaaccaacgc
     1501 gatgttgaaa atattgctat atttttaatt aagattactt cctcaatatc aattatcatt
     1561 ccgatgactg taactttttc aaaagaacag tttaatgtag cggctactta tctttcattt
     1621 atagcgctat tttcttttgt tcttcctaaa agaaataaga gcaactttga caacaaggag
     1681 ctggatgaag tacgcaatag cagcccaaag gaacaaaaat aaaaaaatca tattttagtc
     1741 agtccctccc gactggcttt tttcgctctg tgctcagctt catcaagccc cataaaaatc
     1801 caaaccatgt aaacgattgt ccctatcaac gcttgtctgc ttccccagag gcctaaagcg
     1861 tagatgatta gcggtgcgct gaatactagt gctctgttga atttgcccat taacttgcct
     1921 ccttcaaaac atctgaaggc gataaacttt ttacacgata tttgtttgcg tctttccact
     1981 ttaaaaacca aataaaagta tggtaatgaa taaatgtggt tgagtgacct ggacgaagta
     2041 ttccttctgc aaattcagga attgtttcca tttccttgca gtattcaact aaagtagttt
     2101 ttgacatgcc gtggaacttc tccaagatta aactttgtcg gtaccagtcg tctggtttta
     2161 ctgccttctc agcagcttct atcagctctg aaagtgttgg ttttttcact tattatccct
     2221 cctaaaaatt atcgttccat actcgatgtt taacatttct tgaagctctg attttgtaag
     2281 aatgatttta tcttccaacg actttacctc cctctacaat tcgtacatag tgataatcga
     2341 atctataatt ctgtttgctt ctgcagaagt ctttttaccg tttaaaatta aagataagta
     2401 gcttttgcta attccaaatc tttcagcaag catggtgtaa gttaagaact ttgaactttc
     2461 gacatattct ttgatttttt ctctatctcg ttgagtgatt tctgcaatgt cagtcatact
     2521 aaaactcctt tctaaccaat ttcctctaaa tccatttgag ggtaatagcc ctctgcgatt
     2581 aataagttgt aaataaacac tcgccctttc tgtgtccatt tggtattcat tacaacttta
     2641 gtgccgccat cggctttcgg tatctcactt gtgtgcgatt ttgtgtatcc ttggttcata
     2701 tgttttcggt aaagtatcca ttgtccgcta actttatgct gaacacctaa ctcgtttagc
     2761 aatttgttta gagcaattgc cgacattccg taatcagctg caatttgaga agtagctacc
     2821 gtatctgtcg atgaaagaat cgtatctaag tagctaatct ttggttcgta ctcggcaatt
     2881 tgttgttcga gtaattggtt cttttcttct aaatccgctg ctaaacgcaa tgcttgtgca
     2941 aaactttgag gtacgtttga atagctacct gtttttctaa ttgttggaag gacttcactc
     3001 gttacccaac gtttaaattt tttggcagag ggaagttttg attttaagat taaactgtaa
     3061 aggcctgact cgttaatcaa aacaactgct gttccattga cggtgaacga atcgttctgc
     3121 gtcttatcct ccaagtccac atgatctcga atagcttttt gcggattcga atatcctaga
     3181 acgtctgcta catcttttcc tacaaaatat ggttcatcat ttactaaaat agttcgaact
     3241 tcgttttgct cgaaattaaa aatttgtggt gtattcatat ttctcattcc tttctttggt
     3301 ataatttcct tatcagtgtg acaggctgaa ataattgtta aggaggtgaa aatttatgaa
     3361 accagaattt gtaattacta tgcttgatgg agaaaaaatc aaaatatttg ccgatacttt
     3421 ggtcgttgga tacgatgatg cgtcaaatat cgaagaaaaa gatggaatgt tctattcttc
     3481 tcaaatttac gtgaatacct tgcaaggtga tattggggta tctactcttg caacatcaag
     3541 accgcttgta ggtcttatcg gctttttgtc aaaagcaaca ttcttttctc tcggcgatga
     3601 tatagattcg gatgaaactg tcatctataa aacatcagct gttaaaagta tcggtttcaa
     3661 atagtaattc ttgctcgttg atagcggaag ccaacttttc ttccaacaca tcggcgagct
     3721 ttctttttgt tggattcatt tcaaagttga tagtcttgag aagcattttt gcatccttaa
     3781 ctttttgtcc ttgcaattcc ttgatgattt tatcggcttg gctatcaatt ccatgtgtta
     3841 gatcatctat cattttttgt accttgattg tttggctcat ttccttgttc attgtttagc
     3901 ctcctttaat aaatgatttt ttgtattttt taatttccat aaattcctca aattgctctt
     3961 tggtattatt accacgacca tacatattgt gaaattcttt atggcaactg acacaaagtg
     4021 taacgccgtt tgaaatcgct gcggatttag ttgttgcatg gctaaatcac gaatcacaag
     4081 tatctactaa aggcgaatca atttcgccaa aagaaattgt tcaagcttat ttagatattc
     4141 attatgctgt tatctatggt cagcttccag aagaccgtaa aaacgatgac taaacgtgag
     4201 ctaaaatctc tgctatggct gcaaccatgg cagagtcttc tttatctacc gcgtgttcca
     4261 tcgccacttt tgcttgtttc agtatgtcta atttcaaatc ttcgattttt gccgagactg
     4321 ttttttccat attcttctct cctttctttt atattcgtaa acaaatttaa caacttttta
     4381 aaaaactgcg ttgactttat tagattaata atctatacta aatgcatagt taaataagac
     4441 atataaactt tgatttaaaa agctttcttg gcggttggca tttatt
//
LOCUS       DAZUMB010000168         4416 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000168 DAZUMB010000000
VERSION     DAZUMB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.173"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACG80L_002617"
     CDS             59..913
                     /locus_tag="ACG80L_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5024804.1"
                     /translation="MENSLYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACG80L_002618"
     CDS             1010..3259
                     /locus_tag="ACG80L_002618"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5024805.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="ACG80L_002619"
     CDS             3262..>4416
                     /locus_tag="ACG80L_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5024806.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          626 c          805 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct ctatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acaggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DAZUMB010000169         4365 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000169 DAZUMB010000000
VERSION     DAZUMB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.174"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1034)
                     /locus_tag="ACG80L_002620"
     CDS             complement(<1..1034)
                     /locus_tag="ACG80L_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5024807.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLF"
     gene            complement(1142..3169)
                     /locus_tag="ACG80L_002621"
     CDS             complement(1142..3169)
                     /locus_tag="ACG80L_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS5024808.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3314..4042)
                     /locus_tag="ACG80L_002622"
     CDS             complement(3314..4042)
                     /locus_tag="ACG80L_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5024809.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1218 a          903 c          714 g         1530 t
ORIGIN      
        1 aagaaaagtg aagctactaa gatataaatc caattattgt cttttttaat cgtaacatcc
       61 gtcgtgttca attccttggc tacttttccg tctattgtaa aatctttttc aaatacccat
      121 tgattggtat agttgatcgt agtatttcct ttttttctcg aaaattttcc attttcattt
      181 tcatttccta aaacagtcat tgtaaggtga tattcacctg gggttagcgg ttgaccattt
      241 aaagcagttg gaaaagaaaa atttgtattc ggcgcaatct gcaatccttc tttttcctct
      301 tgatataaaa cttcagaatg tccttttttt gtaatttttg tttcaataac tacttgattg
      361 atataggttt tttgatcatt ttgaacgttt gctaagatga catttcttgc attgatttga
      421 tctggtttaa cttcatgaag agtaaggttc ggagcaactt cgttcagtgt ctgttgcatc
      481 aacaaagcaa taatataggt gtattcattc tcaatcgata agctttgatc cttagtttct
      541 gtttggtcct tttccgtctg gatttcttgg aatgtgatcc caccagccaa tacaccatca
      601 aatcgctcat tcggcatcct gacatgaaaa gcaacggttt ggacactttt aggttttagc
      661 gtcactatct gtggatagct cacataatct accagatcaa agatcaggct catgtctttt
      721 tcaatctcgt tttttccgta ttcaaccatc acatttgaat tggtggttgc gctattcaca
      781 gaaatatcga ttttcacttc tttttctgta tcatttctca attccgcttt tacttctact
      841 tcctgatccg gatcaagttg aagatcgaag taagtttttc tcttgtcgat ttgattctct
      901 gaagttatcg gcgtcactga aaaattaaat gcactagctg acactgatat attgttaaaa
      961 agtgccccta aaataactag gagtgttata aataattgtt gttttggtct caagaaaatc
     1021 gctcctttaa tcatatgtaa gagaagacta taacaaaatc ttgtctctgt gggacacaag
     1081 agcctatggt acctgtgccc ttcttgaaac tcaataattt tgttatattc ttctcttcct
     1141 gttaatttcc tgggacatct gcaagtatcc aagatagttt cccctcaaag gttccgattc
     1201 tttggttttc tacaggaata tctattctga ttccacgatg agctgtagaa tctaaaagag
     1261 aggacaaatc gctttcaccc ttttgatcac tttcgaaaat cgtaacagcg gatgtcccta
     1321 taaccgttgt attttcagag gtaacaaagg aaagagcttc tggaagctca aaatctccat
     1381 ttgtaagaac tttctcttgt ttcaattgta atttccaact tccaaaagcc cgttcatcta
     1441 taattttcaa cccacctgaa aactgtccga attcacttaa tggtctactg gatattttat
     1501 gttttccaaa attgacatat tgaggagcag ttgagagata aagtttgcct tttctgtaat
     1561 aaaaatagat atcctctgta ttttcggaga agatgccttc tgtattcgct gaatccgagt
     1621 caaaaacata cccttcaata tcaataggag tttctttata cttctctcct attttcccag
     1681 tgattgtttt catttcagca atatcaatct gactgtcttt atctttataa taaacatgga
     1741 tatctatctc cttcagcttt gcgatcttta ggtttatttc attcgattgc tcaatcaaat
     1801 tcccgttttc aaggtattca aaatgcatag tgagtacttt ttcaggaatt acttcctgca
     1861 aagttgtctc caattcgatt attaacgtat ctccagcaat aaaataatct gtcagattta
     1921 gcgtgactcg atttccggtt atcaagatat ctttggcatc aatttgattt ccattgatca
     1981 agatatgctc gggaatagtt aattcttgtg ggaggtcgat actagtctgc attttcttat
     2041 attcaagaaa atccccctcg accttcaatg tatttctgat agttatctta tcgtttcctc
     2101 cagcttcagc gatggcccca taatcactat cgttgacttt tacatcagct gttgatgtga
     2161 cattcgatgg aagtcgcgtg acagcgacag cattttcttg aaaaaaagta cctgttgaag
     2221 aagtaaaccc ccaatttact tctgtagaac caaactgcct aatgagatca ggtataatcc
     2281 gagaagagtg accgacacct tccaaatcat aagtaagact tctttgctcc gcgtcccatt
     2341 taattattaa caagcgccat gtgccattag aaagaaatgt aggagctacc gtcacgccag
     2401 tatgctctcc agaataatta ttattatttg gtttaggcgt gacaaaagcg acatgtccat
     2461 gccctctatt atcagcagtt atctctcgat ccattcgatc agaagaacca ctatttttat
     2521 acgtatcaaa ctcaatcgag agcgcattcg caatgtaagg ttggccagaa cgagtagaat
     2581 atgagccaat tcccatacct ggcgatccaa taacttgact gggattacta gccattctag
     2641 gatcgccagt caatgtaaag gtcatgccat ctcccgctct atctcgttga tttcctaagt
     2701 ataaataaga tttaaatgta aaatccgaga gtaaactaat cttcttattt gaccaaatag
     2761 ctcctctttg agacctttct gctgggttca actgcagtaa cttcccattt tccaatatgg
     2821 acgtgctagt accgataggt gtttgaaaaa ttttatcgat agaaataata ttgggatcgt
     2881 caggcagctc aaccgcagaa attggtgcat cttctagtcc ttgtttgtcc gaaccctcag
     2941 cttctacgtt gtcagtggtc tctccactac tcgaaatact taaatcctca ctgctttctt
     3001 gtgtctggtt ggattctatt aaactgtcga atgtcgaagt gagtttgctt tcttcgtctc
     3061 ttctatttcc atgctccaac tgattcaatt ccaaagagta ccctatgatc ggaaagatat
     3121 tagtcactaa gagactgctt aaaatgagca cgatcctcct ctttttcaat atatcttttc
     3181 ctcctgatcg ttaaattcct agatgaacta ttcgtatcca aacaaattta gcctagcctt
     3241 tgacaatttt tgctcgatta cctcgcaaga caatatgact acgtatttat ttttaacagt
     3301 tcttttcagt aaactagttg ttaggtgtat cttctaatgt ccaaactaat ttagtggaat
     3361 attgttgcgc taacttgaca gatttaccgg gtaccgaaag ctgtaccgca tcttgatttt
     3421 cattttcatt tgcaccaaat cgatagaccc aagtccccac acctgttcct gatttagcat
     3481 taattgctaa cgtattgttt cctcccggtg tcaacgttaa ttcagaattc accgtttgcg
     3541 gcgtatatgt tggatcaacc aaagaaacag cctgcccttt cgtaaatttc agctgtgctc
     3601 caactagttc atccccactg gcagttttaa actgttctgg ttgagagaca cttaatttcc
     3661 acccagataa ggtacttctc ctatctgtca cttggacata tgtcggtttt ctagacccat
     3721 cgttcatcac atcagcagct gcataatacg ttttatccgc agtagaaatt ttttgagtcc
     3781 caaatttgaa ttttgatcca taatcaatcg ataatgcccc tcctgttcca gggcttggag
     3841 gatctgatgg atcgacaggt ttattgggat caggattctc tggatcaaca ggaggagtta
     3901 caccctcatc tcctgcttca aatgtaatcg ttccttctgt gttatattcc tttgggtata
     3961 cttcagttgc agaaacaaaa gtcgtacttg ctaccatgct gaataacatc attgctgctg
     4021 ttaatacgat ttcttttttc atcattgaca ttcctctttc tttatttact aaacatatca
     4081 gtcgattgga ttaatccata gcaaaattct gttttacgat acttatgaaa taaaagaaca
     4141 tagacctata tttggtccta tgtatcgatt agttataaat gattatcagc ccgatttgat
     4201 ttctagtttt tattgcaact tgatgcaaac acaatctaga aatacagctg tcatagcctt
     4261 taaaataatt cgtaagtccc cctcctatct atcaaaatgt gacttgactg cctagacttc
     4321 aatcaatatt aattataatt aaataaaaaa tgtatattat atttt
//
LOCUS       DAZUMB010000170         4348 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000170 DAZUMB010000000
VERSION     DAZUMB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.175"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACG80L_002623"
     CDS             complement(387..1853)
                     /locus_tag="ACG80L_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5024810.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACG80L_002624"
     CDS             complement(2144..4228)
                     /locus_tag="ACG80L_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5024811.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1415 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaattt
//
LOCUS       DAZUMB010000171         4324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000171 DAZUMB010000000
VERSION     DAZUMB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.176"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG80L_002625"
     CDS             complement(<1..281)
                     /locus_tag="ACG80L_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS5024812.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="ACG80L_002626"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="ACG80L_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="ACG80L_002627"
     CDS             complement(2359..2721)
                     /locus_tag="ACG80L_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024813.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="ACG80L_002628"
     CDS             complement(2725..3033)
                     /locus_tag="ACG80L_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024814.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="ACG80L_002629"
     CDS             complement(3042..3461)
                     /locus_tag="ACG80L_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024815.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="ACG80L_002630"
     CDS             complement(3454..3687)
                     /locus_tag="ACG80L_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024816.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="ACG80L_002631"
     CDS             complement(3684..>4324)
                     /locus_tag="ACG80L_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5024817.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DAZUMB010000172         4289 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000172 DAZUMB010000000
VERSION     DAZUMB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.177"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            385..1296
                     /locus_tag="ACG80L_002632"
     CDS             385..1296
                     /locus_tag="ACG80L_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFS5024818.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1783..2049
                     /locus_tag="ACG80L_002633"
     CDS             1783..2049
                     /locus_tag="ACG80L_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024819.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2121..2462
                     /locus_tag="ACG80L_002634"
     CDS             2121..2462
                     /locus_tag="ACG80L_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024820.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2517..2936
                     /gene="ssb"
                     /locus_tag="ACG80L_002635"
     CDS             2517..2936
                     /gene="ssb"
                     /locus_tag="ACG80L_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5024821.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3013..3309
                     /locus_tag="ACG80L_002636"
     CDS             3013..3309
                     /locus_tag="ACG80L_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024822.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3324..3755
                     /locus_tag="ACG80L_002637"
     CDS             3324..3755
                     /locus_tag="ACG80L_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5024823.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4060..4242
                     /locus_tag="ACG80L_002638"
     CDS             4060..4242
                     /locus_tag="ACG80L_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024824.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
BASE COUNT         1566 a          663 c          926 g         1134 t
ORIGIN      
        1 actcccccct aaccccctct ttcagcctct tttgaaagag ggggagaatc aaccccacgg
       61 ccaacgcccc agtgttcttg gggctgtcca gaacagaaac ttgcgagaga gtgggggtaa
      121 aacaagtgta gaggaaactt gaagacttgt tcgaacagaa acttgcgaga gagccggagt
      181 aaaacaagtg tagaggaaac ttgaagactt gttcgaacag aaacttgcga gagagttgga
      241 gtaaaacaag tgtagaggaa acttgaagac ttgttcgaac acaaacttac aagagagggg
      301 agtaaagcag ttgcagagga atcaggaaga tttgttcgaa cagaaacttg caagaaagca
      361 ttattaaatt gaaggtgatg aaatatgaag tatttattgc atggctattt tattgattat
      421 tctactcgca atagtagtac tttagcgatt ctatgtttgc ttcatgaatt gagagtggtt
      481 actagaaaac agctatacaa actattgaat tttgacagat caattagtaa acaaggagta
      541 acgaatgctt tagtgagatt atatgaaaaa cagttaatcg atcgaaaaaa atatggaaca
      601 gaaatgtatt attatttgac gaaagaggga catcaaagca taggtgggta ttatacgttg
      661 ccgaaagttc ctgaatataa tttgaatcac catttacaaa ttaacaatta tttgattaaa
      721 ggaatggcat tggcaaaaga ccacccacat ttaaaacgga ttatttcaga aagacgtacc
      781 gtctatgaaa cgaaggattt aaatcctaag acaaaaggaa gaaaattttt tgtgccagat
      841 tttatttttc gttttcgaga caaagaaaat catgagatca attggtcctt tgaaattgag
      901 ctaacgttga aaacacgtcg tcgctaccgt gaagcgattt ttccgaaata tattcgtgag
      961 ttgcgaacac gtcctgaatc acatttaatt tatgtgacac ctagtggcgc aattcaagaa
     1021 gaacttcatg cgtttaagcg tatttttttc aagcgcgaaa ctgaaaaaga tgcgagtcaa
     1081 caagaattgt ttgagcggtt gcatattttt tcagctttag agtttgaaga gggattaaaa
     1141 gcattattag agaatgatcc agcagtcaat tggcggacag tagaacgaga aaaaatttat
     1201 ccgaatggcg ctgttcaaag tcgtttgaat ttatttgaag aatcaactga aaatgaggaa
     1261 aaaccaaaag tgaagtttac gcctgaagta ttgtaaaaga gggtgttcgc ttcgctcttt
     1321 ttttgtgcca gtttgctggc accgttcaga cgcaagcgtc tgaaccggct cgtttttagg
     1381 aaggactaac ttgtttgatt tatccgttgt caaagaagtg gacgttcgag agtccctaat
     1441 cgtatagcag tcattcggcg agtataacct aaaacataac ctgccaattg ccaagggtaa
     1501 aatgcacgct gcgcgccctt ggcaatcgtc agaacatgtt ttcgagccgg ttatgccgaa
     1561 cgaccgctat acgaaaaggg cctctaaaca gaagtccact ttgatccttg acaactgaaa
     1621 cccaaaatca aacaagcagg ccttcgtcta aaaacgagcc gaatgaagac cttgggggtt
     1681 gcacccccaa acccccgtgc ccccgacaca ctaccgaaaa ggtgggggtg ggtggcgaaa
     1741 aaatcaaata aaacattcta ttcaagaaag gaagaaacaa ccatgacaac taaaaacgag
     1801 caactgaaag aacaggcaag aaaagagttg caagaaagag gtttgattat tgaggggagc
     1861 tttgaaggag attttgaaac ctatattggt tgttatgcaa gaccgataaa caaacctaca
     1921 gccttagacc caatgaacga acaagaagta aaagaacaag aaaaacatgc agtgaatggt
     1981 tttccgcaag atttcactga atggtatgag tgggaaatta acaacgggaa gcttgaaaac
     2041 ttcttataaa agtcagatga cgtgcaaaga gaatgattct ctttgtacgg taaaaatata
     2101 aagaaagaag gaaaaagaaa atggcaaaaa acacacctta tcaagtagga gacatttttt
     2161 atagtagttg gggctacgaa caaacgaacg tgactttttg gcaaattgtg aagttaacag
     2221 aaaaaacagc atggtttcag cctattcaaa agaaaacggt cgagcattat tcgtccatga
     2281 gtggaaaagt tgttccaatg aaagacaagt ttatagagta tcctttagca aaaactaaag
     2341 acagtattgt aaaaaaacga attgatccag aaacctatcc tgatatgtta tgtggttatc
     2401 gtagttggga cacgtttcat agatataaaa atcaagctgt ttatgaaagt agttattatt
     2461 aaggggatag tttccccttt caaaaataat aaatagaaga atggagagaa aaaatcatga
     2521 tgaattcagt gaatttagta ggacggttaa caaaagaagt ggatttaaaa tacacaccaa
     2581 aaggcaatgc gacaggcaca tttattttag ctgttaatcg aaattatacg aatgcaaacg
     2641 gcgagcgtga agcagattat atccgctgtg tcatttggcg gaaagctgcg gaaactttag
     2701 caaaatatac tggaaaagga agtttgattg gcattaatgg acgtcttcaa acgaggagct
     2761 atcagaatca gcaaggacag accatgtatg ttacggaagt tctagtgaca gatttctatt
     2821 tgttagagag taaagaaatc aatgagcaac ggtcaaaatc aatggcgaat aatcaaaata
     2881 attcttcaat tccaggaaat gagattcaaa tttcagatag ttatttgccc ttttaagtga
     2941 aaaataattt tgttctatac ctgatttctt caggtataga acaaaaaata aatagagatg
     3001 aggcgatttt ttatgaaaat cattgacagt tacgatcaac gaaccatgca atatttgggc
     3061 tcgtttgatt atacagatga aaatattatt aattatgttg caagtttacc tccttatcaa
     3121 agcgttcgct tggtagattt tgcaagtgat gaattggtat taacaacgat gggcaacttt
     3181 ttagattatg tcggagacaa aaaatggttg gaaaaaattt tgtctaaatt gattgcgaaa
     3241 caaacaggag aagaacccat aaatgcgatt caattgttca atcgatatga acaagaaaac
     3301 aatgattaaa gaggagagaa aaaatgaaaa gattaaaacg aagtattttt attgtgctag
     3361 cattgattga ttttgttggg acaggttgtg aagcaattga cgttcaaaaa gctcccttat
     3421 cggaaataaa acaagcaata acaaaacaag atgaagaaca accgctaaat acacaagtag
     3481 aaatagtgaa tcaaaataaa ccgagttttt ctgaagaaga tttttcgtta gaaaatggaa
     3541 ccaggcaatt tttttcagat ttggatcgtt taaatcgggt aggtgtagca agtgcacttt
     3601 tgcataaaag caggatgcca acagaagaac gagaccgatt atacattaag ccaactggct
     3661 ggaaacaaaa gaaaatgaag aatggcgatt atctttataa tcgttgtcat ttgattggct
     3721 atcaacgtgc gacaagtttc cccataagta tgtaatacga aacgggagga ttaatcttaa
     3781 gattaatcac aactaaccta tttgacagtt ggaatgaaag ctatcatgtc tgctgaatca
     3841 actggtctga tactcctaca tgcaataaag tctactagga agttattgta tgaagctagg
     3901 taaaggcggc agaacaattt ctaagtgtgg aatcatatga agttggatat gccgggacgc
     3961 tataaattac tcagggtgag actgttttta attaaactga cgaacttgcg aatgtacggg
     4021 tctataaccg acttatattc gaaagatata agattgcgaa tgcaaagaac attatcgaaa
     4081 agtaagaatg ttggtcatga aattcgagat ggaagactag cggtgtatct gtcaagagtt
     4141 ggtaaaacat gtttgattga ttcagatgag agtgggaatg cgacaaaacg ttttacagaa
     4201 gaaatagagc aactagcaga actgaatata cgatattttt aaatacttta gcggagaata
     4261 aaaaaatcct ctacagaaaa ctacggagg
//
LOCUS       DAZUMB010000173         4282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000173 DAZUMB010000000
VERSION     DAZUMB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.178"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG80L_002639"
     CDS             complement(37..1257)
                     /locus_tag="ACG80L_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5024825.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG80L_002640"
     CDS             complement(1277..1888)
                     /locus_tag="ACG80L_002640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS5024826.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG80L_002641"
     CDS             complement(1931..2863)
                     /locus_tag="ACG80L_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS5024827.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG80L_002642"
     CDS             complement(3284..4144)
                     /locus_tag="ACG80L_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5024828.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZUMB010000174         4234 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000174 DAZUMB010000000
VERSION     DAZUMB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.179"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..280)
                     /locus_tag="ACG80L_002643"
     CDS             complement(<1..280)
                     /locus_tag="ACG80L_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024829.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKF"
     gene            complement(488..1414)
                     /gene="glsA"
                     /locus_tag="ACG80L_002644"
     CDS             complement(488..1414)
                     /gene="glsA"
                     /locus_tag="ACG80L_002644"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS5024830.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1437..2864)
                     /locus_tag="ACG80L_002645"
     CDS             complement(1437..2864)
                     /locus_tag="ACG80L_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5024831.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2895..4109)
                     /locus_tag="ACG80L_002646"
     CDS             complement(2895..4109)
                     /locus_tag="ACG80L_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS5024832.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1325 a          904 c          761 g         1244 t
ORIGIN      
        1 aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa agagcaatac
       61 catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt atacaatatt
      121 ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc acccttagag
      181 aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca cacagataca
      241 cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca cgcctccagt
      301 ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc tttttattta
      361 gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa acaagctcac
      421 aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca
      481 agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga
      541 taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac
      601 gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat
      661 ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga
      721 ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg
      781 ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga
      841 aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt
      901 aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca
      961 atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt
     1021 catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag
     1081 gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac
     1141 cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta
     1201 ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc
     1261 cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca
     1321 cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt
     1381 ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat
     1441 ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg
     1501 ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta
     1561 gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca
     1621 acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct
     1681 ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca
     1741 acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag
     1801 acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg
     1861 tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt
     1921 gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga
     1981 atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct
     2041 aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg
     2101 gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat
     2161 ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc
     2221 atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga
     2281 atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc
     2341 cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca
     2401 atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc
     2461 aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag
     2521 gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat
     2581 tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag
     2641 acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg
     2701 ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc tgaggtcgcg
     2761 aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa aaagccaaag
     2821 aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct tcctcttttc
     2881 tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa tccttcttct
     2941 tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa aatcactgtt
     3001 gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat ccctaagaag
     3061 taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc tatcagaatc
     3121 gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc atcccattgt
     3181 ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat cccaatcagc
     3241 atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa tccagcaacg
     3301 gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc aatgatcagc
     3361 caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt gactgcttgt
     3421 tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca aagcagtcct
     3481 gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa aggaatcttt
     3541 ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt gtggatgact
     3601 gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc ccatatccag
     3661 tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat atagcctttt
     3721 aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac gataaacatc
     3781 agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat gttggggaag
     3841 cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc gccgatatcc
     3901 cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat cccaacggag
     3961 agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa
     4021 aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat gaatgctgta
     4081 tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg attacgagta
     4141 taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt gcatttataa
     4201 aagacagaca attaataaaa gcttgatgtg ttgt
//
LOCUS       DAZUMB010000175         4197 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000175 DAZUMB010000000
VERSION     DAZUMB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.180"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..907)
                     /locus_tag="ACG80L_002647"
     CDS             complement(35..907)
                     /locus_tag="ACG80L_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5024833.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1035..1604
                     /locus_tag="ACG80L_002648"
     CDS             1035..1604
                     /locus_tag="ACG80L_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS5024834.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1671..2408
                     /locus_tag="ACG80L_002649"
     CDS             1671..2408
                     /locus_tag="ACG80L_002649"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5024835.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2639..3907
                     /locus_tag="ACG80L_002650"
     CDS             2639..3907
                     /locus_tag="ACG80L_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5024836.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
BASE COUNT         1388 a          791 c          853 g         1165 t
ORIGIN      
        1 ttaaaaagct gaaagcgaag gaaaaaaact ctacttaact gtatttctcc gttaggacat
       61 ctaaaaattt acgagcagca ggagaaagag gctggtcttt tttccaaatg agattcagtg
      121 aagcttgtaa tggcggatcg agtgggatga atactttttc cgtcccataa gtattgatga
      181 tcccatctag agctaatgca ccaaaagtcc cgttatcgac catcaaagaa gcattgtaca
      241 ataagttata tgttccgacg atcgataact gatctatgct gaaacctagc cattcagcta
      301 gttgactgtc cactgacgtt tgccgggaaa tgatcaaagg aacagttttc agttcctgcg
      361 gatggatgac tgtttgcttt gctaaaggat gattccgatg tatcaaaagt ccccaacgat
      421 cttgggctgg cagtcgaaat gactggtatt tttgtttgtt tgtcggttcg attacgagtc
      481 caaaatcaag taatccgcta tccagtttgt ctatgatatc atctgcattc ccgctatata
      541 aatgaaactg gatatcagga tgcgccttga tcaacgaatg aaggctatca gaaatgaaag
      601 agattgcttt agtttcgcct ccgccaatgt agatttctcc agagatgatt gcgtcatttg
      661 ttaaattgtt cgccgttttt tctaccaacg acaagatttc ttttcctttt gaataaagat
      721 atcgaccaga atccgtcaac tgtatctctc gggagcctcg agtgaaaagt gtcacaccaa
      781 gttcttcttc taaatctttc agttgttttg agagagtagg ttgcgtgata tgcagagatt
      841 ttgctgctcg gctgatattt ttttctctag ctactgctaa aaaatacgtc agtatacgaa
      901 tatccatttt ttccctcctg tttttaaaaa agcataccac taagtatagt taaaaggaat
      961 agttaagtat gttttataag tattagacat cataagccgt ccgttataaa gtaaggataa
     1021 aaggagggag caaaatgaca agcctcatcg aaaaacaaat cgtcaataaa gagattcgag
     1081 taacggatcc gctctttgaa gtgatccatc agatccaagc ggaaaacgaa gagcctttag
     1141 cagcgctgaa tactgggtac catgaaccag cagatatccg caaacggcta gaaaatatca
     1201 ttagtgacaa agtagatgat acggtgactg ttctgctgcc tttttataca gatttcggca
     1261 aacatattag tatagggaaa aatgtcttta tcaatcgtca agtgatgttc gttgacttag
     1321 gtggcatctg tttagaagat tcagtattga ttggtcctcg tgtcaacctc atcacggtga
     1381 accatctaac cgatccaaaa gaacgaagag gcttgtcagt aaagccaatc cacatcaaga
     1441 aaaatgcttg gatcggagca ggagcaacta ttctgccagg cgtaacgatt ggcgagaatg
     1501 ccattgttgc agcgaatgca actgttacga aagatgtccc agacaatacc atcgttgcag
     1561 gaatccctgc caaaatcgtc aaacctgtcg aacggattat ttgaacagta agatgaaata
     1621 agagatagtg agtataagca agagattttt caaaacagga ggagaataga atgaatgatt
     1681 tatcagaaaa agtgattgtc atcatgggcg cttcaagcgg aatcggtgaa gcaacaacga
     1741 agctattagc agaaaaaggg gctaaactag ttatagctgc acgccgagaa gatcgcctaa
     1801 aagcaataaa agaatcttta ccagaagctg agctatatat tcaaacagca gatgtaagag
     1861 attttgcaca agtccaagca gttatcgatt tagcgatgga aaagttcggt cgaatcgatg
     1921 tattgtacaa taatgcagga atcatgccaa ccgctccatt agttgaaggt caccgtgatg
     1981 aatggcaaaa tatgcttgat atcaacatca tgggtgtgtt aaatggaatt tccgccgtac
     2041 ttccaatcat ggaaaaacaa aaatctgggc atatcatttc aactgattct gtggcgggac
     2101 atgttgttta tcctgattcc gccgtttatt gcggaacaaa atttgctgtc cgtgcaatca
     2161 tggaaggatt acgtcaagag caacggcaaa ataacatcaa atctacgatc atctctccag
     2221 gagctgttca aactgaatta tatcaaacca tcagcgacaa aaaggctgct cttgaacttc
     2281 atgaagcgca aaaagaatgg ggattaacat ctgaagatat agcttctgct gtagcatttg
     2341 cgatagaaac gccagaccgg atgtctgtca gcaacatgat tatccgtccg acgacacagg
     2401 aagtataatc cataacggat aatatccaga gagaattact tgttaaatag ttattcaaac
     2461 gaccatcaat ttaaggcgtg aatggagaag aatattcttc atttacgtct ttttatgcag
     2521 aaaaagcaaa tttattaaaa agaaacaaat gttatttaaa tatggacatt gacttgcttt
     2581 tactttttta gttacactgt tatttaggaa aagtttcata attcaaagga gaaaaaagat
     2641 gaaagcaaag aatttattat ttagcgttat tcttttagga aatctagcag ttgccaacac
     2701 aacagtgcta gctgaagaat cgacagaaac tgtcgcaact cagacatcga gttcttcaga
     2761 aacaggaatt gaaaacgata cagaaagtac tttgcagtca gctactgtct cagaaaatac
     2821 aatttcaacc gaagaaccaa caactgaaac aacagaacaa acacaaaccg aagacagtac
     2881 atcagcagaa gctcagaaca atccagagaa tgcagaagaa atcacgaacc aattgacaga
     2941 tgggcaaaca ttgacacata cccatcaagc agatttgtct gaattcgaaa actatactgg
     3001 ctatgctttt tatgctcgct catctgctaa ttctcaacaa gcgttcattg atagtattgc
     3061 ctcaactgca caaagtttgg caagtgccaa tgacttatat gcatcagtca tgattgctca
     3121 agccatcgtt gaaagtggct gggggaacag tactttggct tctgccccta attacaatct
     3181 atttgggatt aaaggaagct ataatggcca atctgttacg atgcctactt cggaatatgt
     3241 gaatgggcaa tggattacgg tgaatgccgc ttttagaaaa tatccttcgt acaaagaatc
     3301 attgcaagat aacgtaacgg tattgaagac gacttctttt cagccaggcg tttactacta
     3361 ttccggtgca tggaaaagca atacgaactc ttacaaagat gctacggctt ggctaacagg
     3421 tcgttatgcc actgcgccta attatggttc tacattgaac aatgtaatcg aaacctataa
     3481 tctgacacag tatgatacag caccaacttc aacttctccg atgtatcgtc tgtataaccg
     3541 tcatacagga gaacatttat atacattgaa tgccggagaa aaaaattatc tgccaactgt
     3601 aggctgggaa tatgaaggga ttgcttggca agcaccaaac agcggacaac ctgtctaccg
     3661 tttatacaat ccttacagtg gagaccatca ttacacaatg gcacaatctg agatcaactc
     3721 tttaacaaaa atcggttggc gttatgaagg tctaagtttt tattctggtg gttccaagcc
     3781 aatttatcga ctatttaatc caaatgaaaa aactggtacc catcactata ccttgagtgc
     3841 taaggaaaga gattggctaa caccgatggg ctggagatat gaaggaatag gattttatgg
     3901 ttattaaaaa gaggtagtga aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa
     3961 aaacagattt tcatgttttt tgtttggttg aacttattac cgcagatgca agtttttgaa
     4021 caccgtttac tcaaaaaagg ttgtgacaac atcttgtcat aactttttta ttactgttta
     4081 agcatactga tccaatttct aatattcgac gggattcttg caaaaggcga tttcggaaat
     4141 ttttggcggt gtaaaattat ctactgcaga agagctatct tctatagttt gataaga
//
LOCUS       DAZUMB010000176         4136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000176 DAZUMB010000000
VERSION     DAZUMB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.181"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..99
                     /locus_tag="ACG80L_002651"
     CDS             <1..99
                     /locus_tag="ACG80L_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5024837.1"
                     /translation="KRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(352..501)
                     /locus_tag="ACG80L_002652"
     CDS             complement(352..501)
                     /locus_tag="ACG80L_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024838.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            963..1175
                     /locus_tag="ACG80L_002653"
     CDS             963..1175
                     /locus_tag="ACG80L_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024839.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1453..3252)
                     /locus_tag="ACG80L_002654"
     CDS             complement(1453..3252)
                     /locus_tag="ACG80L_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS5024840.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3376..3972)
                     /locus_tag="ACG80L_002655"
     CDS             complement(3376..3972)
                     /locus_tag="ACG80L_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5024841.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1489 a          725 c          705 g         1217 t
ORIGIN      
        1 aaacgaaata atattcctag aaaatcactg gattatcgaa cacctttgga agtatttttg
       61 agttacgtaa gtattgatga tctgtctaac ttaatttgac aattaagatt atattaaata
      121 ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt cataaaaaac
      181 gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag agagacaatt
      241 tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt acaaacgtct
      301 tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc cttatctaga
      361 ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt tattttcgtt
      421 ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc ggtattgcat
      481 atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta gttcttgaaa
      541 gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt tgatgcaatt
      601 aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct attttctttt
      661 aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat ataaatcata
      721 tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga acaacatagg
      781 aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata gttatctaaa
      841 aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt tcaaatacaa
      901 ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa agagggaatg
      961 aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga gtatctcatg
     1021 aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt
     1081 atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc gtaaaattct
     1141 gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga attcgcaaga
     1201 actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa aaagaaaaag
     1261 ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta ttttagctat
     1321 tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg tcaaacaaaa
     1381 aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac ttttttcatt
     1441 aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg aataagtgat
     1501 ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg ttatgatacc
     1561 atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg tccatacata
     1621 tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg tactatattc
     1681 cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag gtaagatgta
     1741 gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt acgtcaaact
     1801 ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa atcgttcgac
     1861 aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt ggtttagctt
     1921 atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc cgttttcagt
     1981 aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat ttacccctgt
     2041 taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg tttcctgaac
     2101 atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt
     2161 attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg gttttgtctg
     2221 aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga aaaaaactgc
     2281 aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg cttgtgggat
     2341 aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata
     2401 aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta aaagcgtcat
     2461 agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca tactttgtgc
     2521 caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat
     2581 aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt cccgaatggc
     2641 tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc
     2701 aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc aacgattagc
     2761 aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat cagaattaaa
     2821 acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca aaaaagtaat
     2881 gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca aagagattgg
     2941 ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga aataaactaa
     3001 cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat gttgagaaaa
     3061 aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta aaataaaacg
     3121 ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca tcagcaagac
     3181 atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta agctacgttt
     3241 caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat gaaaaaggaa
     3301 agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc aatgactaat
     3361 tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat aaatcaaggc
     3421 catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg tatatcgttt
     3481 cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag caactggaag
     3541 aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc agccaatata
     3601 tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat aacgacgccc
     3661 tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga taaatccagc
     3721 ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa tgattggaat
     3781 aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga tggaatcacc
     3841 tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa aataaatcgt
     3901 cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt ttcgagaggc
     3961 agcaatattc attattcatc aactccttag aggttctttg ttaactatac tttactagaa
     4021 aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg taagttcatc
     4081 ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt aataac
//
LOCUS       DAZUMB010000177         4131 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000177 DAZUMB010000000
VERSION     DAZUMB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.182"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..216)
                     /locus_tag="ACG80L_002656"
     CDS             complement(130..216)
                     /locus_tag="ACG80L_002656"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5024842.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            892..1842
                     /locus_tag="ACG80L_002657"
     CDS             892..1842
                     /locus_tag="ACG80L_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5024843.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            1863..2396
                     /locus_tag="ACG80L_002658"
     CDS             1863..2396
                     /locus_tag="ACG80L_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5024844.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            2412..2582
                     /locus_tag="ACG80L_002659"
     CDS             2412..2582
                     /locus_tag="ACG80L_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024845.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            3032..3565
                     /locus_tag="ACG80L_002660"
     CDS             3032..3565
                     /locus_tag="ACG80L_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024846.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            3577..3780
                     /locus_tag="ACG80L_002661"
     CDS             3577..3780
                     /locus_tag="ACG80L_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024847.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
BASE COUNT         1474 a          681 c          763 g         1213 t
ORIGIN      
        1 caaaattgaa tttgattagc aatcatgtta aactgaacct gcaaatgatc aaacattaga
       61 tacacgaaag ccccagagag gtcggattct ctggggcttt ttttcaggtt gatgtcgcct
      121 ttattgattt tacttcttgt ttcggttatt caaccaataa ctgaataaag agacacaaac
      181 ttctacaaga agggggcaaa tgaaccgttc aaacattaga tacacctcct tccagtcgca
      241 agactgtcgg ataggcgaca agttattata ccttacaaaa taaaatctgt gtggtgtttt
      301 taagtatccc ttattttcgg gttgttaggg cttgccctga ccgcctgtaa ggcgctttaa
      361 gaacaagata aggtttttta cccaagactg tcttaaaacg acttagacga gctatcagag
      421 gcttccccca cctccgttac tgacttgttt ttagcaaagg agcctttagg cgactgcgca
      481 gtacaatgtg agagcatagc gatcacactt ttttagcgac cgtagggagc aaggttttga
      541 attggcagtt ttgaaaaatg cgcgaacctg ttggtagaaa aaagaagtgg tttttaggcg
      601 ggaaattttg tcaccctctt ttataatctc tttttaaacc tttttttaaa cctctttacc
      661 gtcggctgtg tccttactcc ttcaatggat acagcgtttt ataaactaca ttttgtctgc
      721 ttttaaacta cattttgtct gcttttaaac tacattttgt ctgcttttaa actacatttt
      781 gtctgctttt aaactctttt tttgcactca cgtatgttat aatatgcatt atcacagaca
      841 aaacgttgtt tataacgtct gtatccattg aaggagtaag gaggaatatc tttggcaaat
      901 gaaatcgtga aataccatca tgaattaaat accattccgt tgcggaaatt tacaccagtt
      961 gaaatgaatc tatttttctc tattgtgtct agaatgcgcg aaaaaggcga taaaacagtc
     1021 cgtcttactt ttgaccaact aaaagattta agtaattata aagcaactgc taataatcgt
     1081 ttcattaatg atttagaaat gacttatgat aaattaatgg atttacgttt tggtaaaaga
     1141 agtgctaatg ggctcgatat agatagattc gttatgttta gtgagtttaa aatacgtgga
     1201 actgctaaag atccatatgt agacatacaa gtttacgaaa aagctttacc tttactaaac
     1261 aatttagatg agtgggttcg ctattcactc caacaattta acgaattaca aagtagctat
     1321 gcaaagacta tgtttcgatt actaaaacaa tatcgaacta aaggttttgc ttatttttct
     1381 aaagaggatt ttcatgaact attagatatt ccaaaaagtt actggaacaa ccaagcaaac
     1441 gttgataatc gtgttataaa gcctatacga caggaattaa ccgcaatctt caaaggatta
     1501 tcaatcaaaa agaaatacgg aaaagggcga ggaaaacctg tcgttggcta tcaatttacg
     1561 tttaaaccag aaatcaaaaa agcagatgat tttaataaac atgcaccaac caaagaagaa
     1621 caaaataaag cattggcttt agctgataaa caaaaagaat acgaagaacc tgctacaaca
     1681 attaacgaaa aaatcaaaca gaagaaacga gaacttgaac tcctccaaca aatcaaacaa
     1741 ctagaaaaag agaaagaagc tcttgaaaaa caaacagagt ccgcactttc gaaaaaagag
     1801 aaaaaaagtt tattatctga tttagataat ttattccagt agcaacctag gaggcaacca
     1861 gtatgcaacc acaagaaacc tttacaataa aggaattgtc agaacttttt aagatgagta
     1921 ggcaagcaat atcgaaacat atccaaaaat tagattcttc catgatagct aaaaatgagc
     1981 gtggttataa ggttgtattg cgttctggag tattgcaact tgctcgcaac ttaggcaacc
     2041 aaatgttgct agataggttg cacgagtacg aacaagacaa cgataaaaat gttaatatac
     2101 caagcaaaaa cgtggttaca aacaagttgc tagatcagtt gcgtgaagaa aatgaatttt
     2161 tacgagaaca acttgccctc agagagcaaa ataattctca tatacaaaaa ctactcgacc
     2221 agcaacaaca actcacccta caagccaaca aacaaatcga aaaactgcaa gaaaaactac
     2281 agttgactta tacagaagaa gctccagaga atgactcaac gactctttct gaagcaacag
     2341 aaacaataga caaacaaatt gatactgaaa aaaaatggtg gcaactttgg aaataactgg
     2401 agggaacatt catgacaaga aaaaatcttc tattcggttc tattttacta ggaattctag
     2461 gattcagtcc gatgttattg tcagtgatca ataaagggaa taaacatggt gaattgaaat
     2521 tgaatccaaa ttattacaac agtcctaacg aagaattgtt tgtaaaagaa acagataaat
     2581 aataaaaaag actgctcttt cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa
     2641 aaagcaacgc gcttatgaag cgctaccaaa aatttatcat attttattaa aaataacaac
     2701 ccagtaaaaa atgactggta aaaattcaca aagaaacgga aagaagcaac cgcagaaaat
     2761 caagcttaaa aagagctttt gacctagacc aatcgaaaat cacaaagtga gcgcctgtat
     2821 ctattgctac aggcgctact tggcatagtg tacattatgt caacaagaat ttaatataac
     2881 atattttcct aatgcattct aactatacca cattatcaaa aatataaatt atctttacta
     2941 aataaaatat tgacaagcct ataccttttg ttttatgatt ttcacaaaga aaacgttttc
     3001 tatatatttt tctgtatcag gaggaaattc tatgaaaaaa tttactttag gggtagcttt
     3061 gttaactatt ggggcagttg ttttaccgtc aactactata gtttatgctt cagaaaattc
     3121 ccaagtggta tcattaaacg aaaacaatgc tactgaaaat gtagttattg agtacactga
     3181 agatggtatg gtcgtaacta aatatgagga tggttatagt gcggaagatt ttgcaaattc
     3241 ttcagatatc atgacaagag ctgcagttgc aagatggggc aaaaaggaat atacaaatat
     3301 tgctgtaacc actggagtag ctgctaatgc tattaatgct gccttttggg caggagggaa
     3361 agctagcact tctatgtttg gtatcccagg ttgggcaatt ggtgctttgt taacaggtgc
     3421 aagttggact aaaaaaggtg cttctccagg gaatgcagtt gcaaaagaat gggacaaaaa
     3481 tggtaatggc tggattggat tttattacca aaggggatat gatgcagctg gaagagttgt
     3541 agctactaga tattcaacgt tataagttaa ggagtgatgt ttttgatatc taaatcatta
     3601 aaatcattaa aaacatttac taaaaagaat ccgcaaatag ctactattcg tatttttata
     3661 tttgctatag ctgttgctat aattgttaat atgctaagcg gagggcaatt atacaaatct
     3721 ggtttatttt tagtgttaat cttagttgtc ttagacataa taaagtctaa aaaaaaataa
     3781 atcgatacat tttagaagaa tagggttgtt tgaatattac aaagcaacaa tttcaccttc
     3841 ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt ttttgtattg
     3901 tggagcgtgg attttgaggg ggattcaagg gggagcttgc gctcctttga caagcggtta
     3961 tttgcaccaa atacggaggt atttggctag caaatataga gcttgccaaa cccttttagt
     4021 gagctttact cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga
     4081 cgtactgaaa gggtgggact tatgagcgaa caacatcaaa atttggctag c
//
LOCUS       DAZUMB010000178         4128 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000178 DAZUMB010000000
VERSION     DAZUMB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.183"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..743)
                     /locus_tag="ACG80L_002662"
     CDS             complement(204..743)
                     /locus_tag="ACG80L_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024848.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            complement(883..1563)
                     /locus_tag="ACG80L_002663"
                     /pseudo
     CDS             complement(883..1563)
                     /locus_tag="ACG80L_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(1885..2106)
                     /locus_tag="ACG80L_002664"
     CDS             complement(1885..2106)
                     /locus_tag="ACG80L_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024849.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2204..2449)
                     /locus_tag="ACG80L_002665"
     CDS             complement(2204..2449)
                     /locus_tag="ACG80L_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024850.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2689..3303
                     /locus_tag="ACG80L_002666"
                     /pseudo
     CDS             2689..3303
                     /locus_tag="ACG80L_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(3673..4002)
                     /locus_tag="ACG80L_002667"
     CDS             complement(3673..4002)
                     /locus_tag="ACG80L_002667"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024851.1"
                     /translation="MDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKS
                     GILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNL
                     DESDLPF"
BASE COUNT         1335 a          743 c          591 g         1459 t
ORIGIN      
        1 atatttaata tatatgaaaa ggaaaacgta gaaaattacg tctacgtttt cctatttagt
       61 tctatatttt cattaaattt tatatttgct cttttcactt ttcggaataa attcactgtg
      121 actcttgata ccttctttta tatattgcta atgtattttc tccattgcta tgaaaataga
      181 atcaacatat ttataatatc tatctaatct attttcattt caccgcagca tcttattaaa
      241 taacgtgtaa ataagaatat aaaagttatt gaactaatta ataaagatag aatcatcgta
      301 gttggatacg tagatgaaac ctcaactata gttcttttcc ccgtaccttt aatagacact
      361 aaaccattgt cattttctat agtgctcttt ttcccattta ctttcacaac tgttccccaa
      421 taattgaata caggtaatgt gatattttgc tcatcggcat cttctactgt atagactatt
      481 ttggaattat atgctttatg agatgcattc tcccaccgat caccaacttt tacttcatgt
      541 ttctttaaat gatctttata tttcaatgca tctaatggtg catagtcata taggcctcca
      601 gttcttgagg atgtataacg tgtaacatct tttttggtca taactaaacg acctttatac
      661 gactgaattt tatgcgaata atttgcttct gttgctgcag tcacagtaaa aataataaga
      721 actattccta caaggctcca catacttttt ttgttgcttg ttttccactt taaaaacact
      781 atggttaaga ttaatgaacc aaaaagcact tgaaaaccca aggttctcgg ttctgttgcg
      841 aagtttgaaa atcaaacgcg ccctctctga actccaaata tcttaggctg ttattcccat
      901 taataccttg atttcagtag acaccgaaaa gccgaagagc gttccatttc ttcggttctt
      961 tttatatatt cctcgaatgg tctccatgcc cttaatcgtg gaagaggctg tacggagact
     1021 ttgataaaat ttatttcgtc gtttaatagg tcgatggtct tgttctatta aattgttaag
     1081 atacttcaca gttcggtgct ctgtcttagt atataaaccc acactctgta actttctaaa
     1141 ggcggagcca agagaaggtg ctttatcggt cacaattgct ttcggctcac caaactgttt
     1201 atggagtcgt tttaagaaag cataggctgc ttgcgtatcc cgtttctttc gtaaccagat
     1261 atctaaggtt aagccgtccg catcaattgc acgataaaga taatgccaac gtcccttaat
     1321 tttgatatag gtttcgtcca ttttccatga atagaaggat tgtctatttt tcttcttcca
     1381 aagataatag aggactttgc tgtactctta tacccaacga taaatcgtag tatgacaaac
     1441 atttattcca cgatcatata acaattcctg aacttcacga tagcttagat tgtaacgcag
     1501 gtagtaacca acagcgacaa taatgacgtc ttttttgaat tgtttgcctt taaaatgatt
     1561 cattactctg tcctctctgt cttttttctc aattttacac taaaatagat tttttggaaa
     1621 actttgcaac agaaccgttt ttactatata cttcttccca gaacttgtcc gtctccttca
     1681 aattatctct acctctcgag ttcattgggt ggtttgccca tttattaaac atggaatcac
     1741 ctttctcata ctcatatatt tatgtcctat ttcaattata tttatatatg cttaaataaa
     1801 tttgtatcct gaaatttatt gtaatatgaa gctgtatttt acatatagta accaaagagt
     1861 taataaaata aaaagagcga accattatag atcagctctt ttttcattgc tgtttttaat
     1921 cttatctaga atcacttcaa atgtttcaaa ggtatttaca tctaactgac ataaccaaag
     1981 caagtacttc ttttctttgt aagtccaatc aatattacgg taagaggaca ttagattatg
     2041 gtaccttgct ctttgttcaa ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc
     2101 ttccattatt gatccctcgc aagacgctac ctcaaactta aataatgggc agcgaaagtc
     2161 taattcctta gatctaagta tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag
     2221 ttcttccgtc ttttgctttg cttctattct taatgtgttt cgttatcggt gccctacaaa
     2281 gattttcatt aattacacac tcatttattg ttgtgtattt tctgacattt ctctcttgat
     2341 caattatttt aattccatgg gcatcctgat agttcatagg agaggagcgg ctatcagaaa
     2401 tagttgcacc tatctcttga acttctttta caacttctgg aaaatccatc catcctgctt
     2461 cagctgtcaa aagtaataat ctataatatt tatctttcct tttcaatcta agaaatcgtc
     2521 accccattct ttttcttcta ttataaacta tttaactttt agaattcaaa aaccgtttgt
     2581 ctatttagag tttaccctaa atagacaaaa atataacatt gaaaaattta gagttctaac
     2641 gtatgattag ggtgtattat aaacgttata gaaaagtagg gaataaattt gaaaattgct
     2701 tatattagag tttcatccat cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag
     2761 tttggcgctg aaaaaatttt tatcgaaaaa caatctggag cgacgattag tcatcggccg
     2821 attttccagg aagctttaga ttttgtcaga gagcaagata tttttattgt agaagccatt
     2881 gatcgacttg gacgcaatta tgacgagatt atcgattcag tgaattatct aaagaagaag
     2941 aatgtgcgac ttattatcac cagtttaccg atcatggctg aagcaattgg aaatcctctc
     3001 ctagataaat tcatcaaaga cttaatcatt taaatctcag ccatgattgc tgaacaagaa
     3061 cgtactgaat cgaaacgacg ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc
     3121 tataaaggtc gtcctaagtt atacagtgca gacaccaaag atcctcaacg tcgccttgtc
     3181 tataggagta tcgttcagga tcttgagaat ggggttgcta tctcaaagat tgcaacagac
     3241 tacaatgtga ctaggcaaac aatttaccga ataaaaaaag aaatcgacca actaattgta
     3301 tgatttcttt tttgaatcac gtaattatta attagctttg tcatcaatac agacaatcat
     3361 tttagattca tctagctaag ttgattacat ttatgttaaa aagaatttag ttaacataaa
     3421 tataattgat gcataaatcg tccttattta aaggcattta acattgacaa cttagtataa
     3481 tgtatcttat gtaaactaat aagatttgaa ttttatttta tacacactcg ataatagatc
     3541 aattttatgg tcccccgcat ttacgattgc acaagagaaa cataaagtcg acctattagc
     3601 atttcttttc aattcaaaca ttaaaaaagt ttatacctct gctactaaaa aatgaaatta
     3661 tctatttaaa atttaaaacg gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg
     3721 tattggagct ttgtatttct ccataataac agatatcttt ttttcatttt ttaaaaaata
     3781 ccttttaaat ttcttatagt cctctgaaat caaaatatag cgtgatggat actcatcaaa
     3841 agaagaatcg tattcttcct ttaaaattcc cgatttactt aatttttcta gtacatcaaa
     3901 tactctatca ctcaagtaac tccttaaatt gttatcactt tcccattgaa ctacttttct
     3961 ataagttgaa ttatcagttg aaaagataga attttcatcc ataaacgcat atattagcag
     4021 caatctttca tcattgtcaa attctattgg atgaatcggc tacactagac tagacagaaa
     4081 aaataaggtg tgtagaataa taaaaaacac actggaggat ttttcatg
//
LOCUS       DAZUMB010000179         4085 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000179 DAZUMB010000000
VERSION     DAZUMB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.184"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..909
                     /locus_tag="ACG80L_002668"
     CDS             <1..909
                     /locus_tag="ACG80L_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024852.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
     gene            923..1072
                     /locus_tag="ACG80L_002669"
     CDS             923..1072
                     /locus_tag="ACG80L_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024853.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="ACG80L_002670"
     CDS             1069..1515
                     /locus_tag="ACG80L_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024854.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="ACG80L_002671"
     CDS             1517..1654
                     /locus_tag="ACG80L_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5024855.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="ACG80L_002672"
     CDS             1691..1984
                     /locus_tag="ACG80L_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024856.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="ACG80L_002673"
     CDS             1981..2205
                     /locus_tag="ACG80L_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS5024857.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="ACG80L_002674"
     CDS             2202..3221
                     /locus_tag="ACG80L_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5024858.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3410..3483
                     /locus_tag="ACG80L_002675"
     tRNA            3410..3483
                     /locus_tag="ACG80L_002675"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3444..3446,aa:Ile,seq:cat)
     gene            complement(3848..4054)
                     /locus_tag="ACG80L_002676"
                     /pseudo
     CDS             complement(3848..4054)
                     /locus_tag="ACG80L_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1399 a          717 c          818 g         1151 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
     3481 acattaacgt agccatttga atcgttctgt gttagaattt tttgaagagt attatacaag
     3541 ctaaagcttt tcttcattgc cactcaaatg agtggctttt ttatgtatcc ttttatggat
     3601 taatgaaagg atgtttcaca tagttatatt tctgtatatt tgaaaagttt tactttgatt
     3661 tttaaataga aagacatttg ggttatattg tgagataata ataaagaaga gtttaaagcg
     3721 ttccccaaaa accactgccc cataagtgtg ttacgcttta aactctttta tatttgaagc
     3781 cattaaaaag cataccatat ttttgaaaaa aagtgagaaa aaaggcttat aattggagtg
     3841 gtagttattc tataacatca agtttaaatt ttgtagtgta gcttttgcca cctccaacca
     3901 agccttccca accatgatag ttataaatcc ttacccaatg tctaaccagt gtacgattta
     3961 tttgatattt atgtgcaaga tatttgtagc cgccttcgtt atttaaatag tctgaaacta
     4021 cttttttctt aaaaacaaat gtatatttcc gcaaaaaaag cacccctttt aattagattt
     4081 ctagt
//
LOCUS       DAZUMB010000180         4001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000180 DAZUMB010000000
VERSION     DAZUMB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.185"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(110..1615)
                     /locus_tag="ACG80L_002677"
     CDS             complement(110..1615)
                     /locus_tag="ACG80L_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5024859.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1612..2298)
                     /locus_tag="ACG80L_002678"
     CDS             complement(1612..2298)
                     /locus_tag="ACG80L_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5024860.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2491..3912)
                     /gene="gndA"
                     /locus_tag="ACG80L_002679"
     CDS             complement(2491..3912)
                     /gene="gndA"
                     /locus_tag="ACG80L_002679"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS5024861.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1099 a          850 c          687 g         1365 t
ORIGIN      
        1 ttaattgttc gctagttttt ttataatgac atccctgaat ttctcacttg cttgtatttt
       61 gtaccttatt ctttgttgga tatataaaaa aacaagtaaa gcacatgctt tacttgtctt
      121 ctgcctttgc gataggaata aagattctaa aaattgtccc ttgccctact acactttctg
      181 ctaagatacg tcctttatag ttttcaacca gttgtttagc gatagacaac cccagaccat
      241 ttccaccctt cgttctagct cttgctttat ccactcgata aaaacgattg aagatcttat
      301 taaggtcttc tttgggtatt ccttcgccaa agtcctgaat cgcgatctca aattcattca
      361 gagtggaaga aatagagata tggacttctt ttcgtgtagt tgaatacttt actgcgttat
      421 ccaatatgat aatgattaat tgttcaaagt gattacgata aatttgcagg gtcacttctt
      481 ttgtcaaatc atcatctaaa gtgatcacat agtctggata taatatcttg aaattattat
      541 aaacttgata ggtgacttct tttcctttag ttgtttcatt cgcataatga acatctactt
      601 gttctgctct tgataagtct agcatctctt gaactaagct tttcatacgg ctgatttctt
      661 gcatgctggc gcttaatgat tcttctaata tctcaggatc atctttcccc caacgattta
      721 gcagactaag atgcccttca ataattgcaa caggtgtcct taactcatga gatacgtctt
      781 ccacgaattg ctcttgttgt tcaatataca aacgcatacg gtcaagcatc tcattgaata
      841 tttcagctaa atctgccaat tcatcatttg tgtcgatttc aggcatgtgg atatctgatt
      901 gcggatcttt acgtatagta tccattgtat ctctgagtac cttgagtggt tttaagaaat
      961 aagaagataa aaagaaacct aaaatactac ttaagatcaa agaaacaact tctaaaacaa
     1021 taagagtcaa taatagctta tttcgaatat catagaaaga agataattca tagaagcttt
     1081 gcgcatagcc gatcttttct cttgtacctt tagaatatat aggctggata gataaaaagc
     1141 cagtttttcc ttctagtgta acgatcgttg gaactttacg tgtagtctga atcaggtttc
     1201 gactgttttc atgggtttta aaaatcaagt tctcgtccaa atcataaaca taaagatcca
     1261 ttgatggctg ccctagctca gctataaagg aatcgatctt cattaagttt ccttcgacca
     1321 gtgaagtatg atctacgacc tcatctccgt catttgtcgc tttcgtcaga ttacgatacg
     1381 tatttactaa tgtcagttct tcatccgagt tagccaaacg agaagctact tctgaaatcg
     1441 tacgctctac attgtttctt tcttttgcca caatcagatt gatagatgat ttataggtaa
     1501 tgacagcgaa aattgtaaac acaacgaata tgaagaaaga actcgcaaag gcccacttga
     1561 ttgtcaagga gggccctttc agttctcttt tttccgattt tttcggcttt atcacgagcg
     1621 catcacataa cctgttccgc gtacagtttg gatatagctt tcctccccgg gaacgtcaat
     1681 tttgttgcga agataacgaa tatatacgtc cactacgttt gtttcaactt ctgtttcgta
     1741 tccccacact ttatttagta aaacgtcacg tgctaatacc acgttcacgt tttccattaa
     1801 agtaagcagc aactcgtatt ctctttttgt tagttcaatt acttcatttc cgcgacgtac
     1861 gacacggttt tctttttcga tagtcaaatc gcgataagta atagtagttt gtttagccac
     1921 atttttatcg ccttcaatat cgattcgacg caacaatgca cgaagacgtg ctaataattc
     1981 ttcgatagca aatggtttga cgatgtagtc atctgctccg tggtcaagtc cagatacacg
     2041 atcgatcaca gaatctcttg cagtcatcat aatgattggt gtatttttga cttggcgtac
     2101 acgtctgcag acttctaaac cgttcaattc tgggagcatc agatccaaca atatggcatc
     2161 ccactcatta tttaatgccg catctaaacc agttcggccg ttgtagtgga cttctgcgtt
     2221 atacccttca tgcttcaact ctagctcgac aaatcgtgcc aagttctttt catcttcaat
     2281 gattagaata ttgctcattt attattttat cctttctatt ctactggctt tttaaaaact
     2341 ctatcattat accgataata tgcttaaaaa tctatagtca ttttatcatg gatagcaatt
     2401 ttaaaaaaga gctgaagtct actcataaca atcgacttca gctctttcgt cctcttttta
     2461 ttttctcatt tcctaacagc cgaaagacta ttattcttca tggtaccatg agtaatggaa
     2521 gattccttct ttgtcgacac gctcataggt atgtgcgcca aagtaatcgc gttgggcttg
     2581 gattatattt gcaggcagac gttctgctct gtatgaatcg aagtaagcga tggctgatga
     2641 gaaagtcggt acaggtacgc cagcttggac agccaaggca acgacgtctc tgactgattg
     2701 ctgatatttt ttcgtgattt caacgaagta atcatctagc aataaatttt cgatctcagg
     2761 atttttgtca taggcatccg taattttttg caagaatcga gctcggatga tacatccagc
     2821 gcgccagatt tttgcgattt caccaaatgg caagtcccaa tcaaattctt ttgaagcgac
     2881 acgtaattga gcaaatcctt gggcataact catgatttta ctgaaataca acgcttctcg
     2941 gattttttcg accaattctt ttttgtctcc cgtgaactca aaatcgttcg tacgagacaa
     3001 aatcttgctt gcttgtacgc gttcttcttt ataagcagaa atatagcggg caaatacaga
     3061 ttcagtgatc aatggaagtg gcacacctaa gtcaagcgcg ctttggcttg tccatttacc
     3121 tgttccttta ttcccagctg catcaaggat cacatctact actggtttac ctgttccttc
     3181 atcgtcttta cgtgtcagaa tgtcagcagt gatttctatc aaatagctgt ctaattcgcc
     3241 ttgattccat tctttaaaga tatcagccat ttcttctact gaaaggttta aaatattctt
     3301 cattaagtca taagattcag caatcagttg catatctcca tactcaatac cgttgtgtac
     3361 cattttcaca taatgtccgg ctccatttgg gccaatatat gtgacacatg gtgctccgtc
     3421 ttctgctttt gcagaaattt gctctaagat tggtgcaact agcgcatagg cttctttttg
     3481 tccaccaggc atgattgaag gtccttttaa agctccttct tcaccaccgg aaacgcctgt
     3541 accgataaag ttgatgccag agttggctaa ctcttcatta cgacgcatcg tatctttaaa
     3601 gaatgtattt cctccgtcaa tcaaaatatc gcctttatca aggtgaggaa gtaactcttg
     3661 gattgttgca tctgttgccg gtcctgcttt gaccattagc aagatccgac gtggtttctc
     3721 gatagattct acaaattctt cgattgtata tgttgctttg aaatttttat ctgggtgttc
     3781 ttctactaca gcggttgttt tagcccctgt acgattgaat aaagccactg aatagccgcg
     3841 gctttctatg ttcaaggcta gatttttacc cataacggcc ataccgacaa cgccgatttg
     3901 ttgttttgac atatgaactc ctcctacata cgggatttct ccctaatctc ttacattata
     3961 gcttaacacg gctacattgc aaactaaaga acaacatatt t
//
LOCUS       DAZUMB010000181         3950 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000181 DAZUMB010000000
VERSION     DAZUMB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.186"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..503
                     /locus_tag="ACG80L_002680"
     CDS             <1..503
                     /locus_tag="ACG80L_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFS5024862.1"
                     /translation="GIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGVLVILEGGMII
                     DTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVMAPLGDLMNVS
                     RDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFAILTLVSAVLL
                     YIATLF"
     gene            536..1381
                     /locus_tag="ACG80L_002681"
     CDS             536..1381
                     /locus_tag="ACG80L_002681"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5024863.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTYSNCQAVKQLAQKHALDIIAFTPESIIYPYNIAAPETMQWEKSKAYFRNAILA
                     TAELGCSLMTINSGYGLMEEPIEESWKRSREMLRYLAEIAETEGVTIALETLRPEESK
                     IVTKLQEAQRMVHDVAHPSLKLMVDTAAMGVAGEGLQDWFKAFGDDIVHLHFVDGTPE
                     GHLAWGDGNFNLHEFLTILEKYDYQGFLGQEITDSRYLKRPYLADKQVFDKLSEALRK
                     REGNV"
     gene            1378..2166
                     /locus_tag="ACG80L_002682"
     CDS             1378..2166
                     /locus_tag="ACG80L_002682"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5024864.1"
                     /translation="MKDFVAVGYACMDIYDDLGKKYPTGNGVDVIAHLSKAGYDVALV
                     SAVGDDTYGKEFIEFCEDMEFDTSHVHVEEGDTAQIHLSLIDGDRVHGKVIDGVLANF
                     SLTKEDRAFIKEFKYLHTDYFGRIYGMLGEFQQAGCKIVFDFSSFINDKDFEMVAPYV
                     DIGQISVEGFSQETKKIMQYMHCLGIKICIATAGSQGALAYDGKNFYCQEALDPQEIV
                     NTVGCGDAFLAGFLMGDAASKPIEECLLLGAKSSQKIIQKFEPY"
     gene            2187..3050
                     /locus_tag="ACG80L_002683"
     CDS             2187..3050
                     /locus_tag="ACG80L_002683"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS5024865.1"
                     /translation="MTIDTCFHPIYFDMIQTSEAELKKREEAFGVHKQSPYTMDEILS
                     EMDYGKIEKSILHPIALTTTENVSIVTNEEISKIIQQEPNRFIGFASVDPNNPEAETE
                     LEQAFQKGLAGLTFHPGKQQIYPNDPKMKPLYQLCEKYDKPIYFHSGLSWEPRSYTKF
                     CQPLFVEDVLIEHPALRICLAHFSWPWIQEVMMLMIKYSNLYTDTSMLYLNSFDDFFG
                     DFFSNQLTPSTIERNFPRQIMFGSNTPRFRAFKVKRALEKIPFSKEVRENILTNNAYD
                     YVYGRKSRHDL"
     gene            3040..3468
                     /locus_tag="ACG80L_002684"
     CDS             3040..3468
                     /locus_tag="ACG80L_002684"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFS5024866.1"
                     /translation="MIYKEYLEFFSTEDVQLINITEEVEKVMARSNITSGVAFILSNH
                     TTTGITINEPLECVEKDIKNTLERLIPENSNYIHAHFLPSYGATSNNSPGHLKSSLLG
                     NHAMFSVEDGKLEKGSAQDIYLVECDGPQTRKILIKILGE"
     gene            complement(3767..>3950)
                     /locus_tag="ACG80L_002685"
     CDS             complement(3767..>3950)
                     /locus_tag="ACG80L_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5024867.1"
                     /translation="NGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPI
                     EIFLSYVQEAFYSSLI"
BASE COUNT         1228 a          676 c          825 g         1221 t
ORIGIN      
        1 ggggcatcgc tagattttct caaaaggaaa ttgctgaagc ttttgtcgaa ggctgttcag
       61 atttcgccta tgctgctgta atcattgggc tagctagagg agtcttggta atcttggaag
      121 gtggaatgat tattgacact attctaaaca gcttggctaa ccttttaggt ggtcttccaa
      181 aattcctgtt cactacgatt ctactcattt ctcaagtaat tgtttccttt tttgttccat
      241 cctcctcagg agctgcggca ttaacgatgc cggttatggc accattgggt gatctaatga
      301 atgtaagtcg tgatgttgtt gttctaagta accagtttgg aaatggttta atgaatatta
      361 tctccccaac tagtggtgta ttactggcag gattagccat cgcagagatt aatttctcta
      421 aatggtttaa ggttggcttc aaaatatttg ccatcttaac ccttgtttct gctgttttgt
      481 tatacattgc tacactcttt tagtcttaat catccattaa taggaggcaa tagttatgat
      541 taaaataaat caattagcag gaatgaatat ccactattta ttttattctc tcaaagaatt
      601 tttcgcttat caacagaaaa ttggtatttc ttctgttgaa ctttggggag gtgctccgca
      661 ttttttcata gatgctttta cttattctaa ttgccaagcc gttaagcaac tagcacagaa
      721 acatgctctt gatatcattg cttttactcc agaatcaatt atctaccctt ataacattgc
      781 tgcgccagag acgatgcagt gggaaaaaag caaggcctat tttcgaaatg caatattagc
      841 tacggctgag ttaggctgct cactgatgac aatcaactcc ggatatggtt tgatggaaga
      901 gcctatcgaa gaaagctgga agcgctcaag agagatgcta cgatacctcg ctgaaatagc
      961 agaaactgag ggagtcacga ttgcattgga aacgttgcgt cccgaagagt cgaaaattgt
     1021 aaccaaatta caggaggctc agcgaatggt tcatgatgtg gcacacccat ctttgaagct
     1081 gatggttgat accgcagcaa tgggggtcgc tggagaaggt cttcaagatt ggtttaaagc
     1141 cttcggggat gatattgtcc accttcattt tgttgatgga acaccagaag gacatcttgc
     1201 ctggggagac ggaaatttta atttacatga gtttcttact attttagaaa aatatgatta
     1261 tcagggattt ctgggacaag aaatcacaga tagccgctat ctaaaacggc cttatcttgc
     1321 agacaaacaa gtatttgata aattatcaga agcattgagg aaaagagagg gaaatgtatg
     1381 aaggattttg ttgctgtagg ctatgcatgt atggatatct atgacgattt agggaagaaa
     1441 taccccactg gaaatggagt ggatgttata gctcatctct caaaagctgg ttacgatgta
     1501 gctcttgttt cagcggtagg agacgacact tatggtaaag aattcattga attttgcgag
     1561 gatatggaat ttgatacttc tcatgttcat gtagaggagg gagatacagc acaaatacac
     1621 ttgtctctga tagatgggga tcgagttcat ggaaaagtaa ttgatggtgt actagcaaat
     1681 ttttccttaa caaaagagga ccgggcattc atcaaagagt tcaagtatct acacacagat
     1741 tattttggcc gcatctatgg gatgctagga gagtttcaac aagccggctg taaaattgta
     1801 tttgattttt cttcttttat caatgataag gattttgaaa tggttgctcc ctatgttgat
     1861 attggacaga tttctgtaga aggattctct caggaaacta aaaaaattat gcagtatatg
     1921 cattgtttag gaatcaaaat ctgcattgcc actgctggaa gtcaaggggc tctcgcctac
     1981 gatggaaaga atttttattg tcaagaagcg cttgatcctc aagaaattgt caatacagtg
     2041 gggtgtgggg atgctttcct cgcgggtttc ctaatgggag atgcagctag caaacctatt
     2101 gaagaatgtt tgttattggg agctaaaagt tcacaaaaga tcattcagaa atttgaacct
     2161 tattaattga cagaggagcg gaagtaatga ccatagatac ttgttttcat cctatctatt
     2221 tcgatatgat tcaaacatca gaagcagaat taaaaaaaag agaagaggct ttcggtgtac
     2281 acaaacaaag cccctataca atggacgaaa ttctttcgga gatggattat ggaaagatag
     2341 aaaaatcaat tcttcatcca attgctctaa cgactaccga aaatgtcagc attgtgacaa
     2401 atgaggagat tagcaaaatt atccaacagg agccaaatcg ttttattggt tttgccagtg
     2461 tagatcccaa taatcccgag gcagagaccg aactggaaca agcctttcaa aagggacttg
     2521 ccggtttaac tttccatcct gggaaacagc aaatttatcc gaatgatcct aaaatgaaac
     2581 cgctttacca gttatgtgaa aaatatgaca agccaattta ttttcactct ggtcttagct
     2641 gggagccaag aagctacaca aagttctgtc agccactatt tgtggaagat gtattgattg
     2701 aacatcctgc gcttcgaatc tgtttagcac atttttcatg gccctggatt caagaagtga
     2761 tgatgttgat gataaaatat tcaaatttgt atactgatac ctctatgctt tatttgaact
     2821 cttttgatga cttctttgga gattttttta gcaatcaatt gactccctca acaattgagc
     2881 gaaattttcc aaggcaaatt atgtttggat caaatacacc acgttttcga gccttcaaag
     2941 tgaaacgggc tctggaaaag attcccttct caaaagaagt gagagaaaac atcttgacaa
     3001 ataacgctta tgattatgta tatgggagga aaagtaggca tgatctataa agaatactta
     3061 gaattctttt ccacagagga tgtccagtta ataaatatta ctgaagaggt agaaaaggtt
     3121 atggcgcgga gcaatataac aagtggcgtt gcctttatcc ttagtaacca tacaacaacg
     3181 ggcatcacaa tcaatgagcc actggaatgc gtggagaagg acataaagaa tacactagaa
     3241 cgattaatcc ctgagaattc gaactatatt catgcacatt ttcttccctc atatggagcg
     3301 acaagtaata actctccagg acatttaaaa tcctccttac taggaaatca tgcaatgttt
     3361 tcagtggagg atggtaagct ggagaagggg agtgcccaag atatttattt agtagaatgt
     3421 gatggacctc aaactcgcaa aattctaata aaaatactag gagagtagtt ttgaattacc
     3481 ggaagggact gggacaaaag ttttttctta aaaaatagtc ccatttcttc agtattaatt
     3541 aaataaaaag taaaatacat tcccgaaact attggatgaa gatcccagta gtttcgggaa
     3601 tgtatttttt ctagaggctt tcttttgctc tttgtcaaga agtgttgatg acgagatttt
     3661 ctaattcaaa tgaaggataa tttcacaatc tattaagcag tggctaggaa ccaattggtt
     3721 cctaagttgc tgcttttatt tctaataatt tatattctat gatttgtcaa attaagctag
     3781 aataaaatgc ttcttgtaca tagctcaaaa atatttcgat tggtgttctg taattcaatg
     3841 attttcttgg aatatgatta cgttgattgc tgacactgga aataaatgtc tgattcactt
     3901 ctctaaaatc tattgatttc ggcagtccat tacgacgcag aatcccgtta
//
LOCUS       DAZUMB010000182         3922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000182 DAZUMB010000000
VERSION     DAZUMB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.187"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..645
                     /locus_tag="ACG80L_002686"
     CDS             <1..645
                     /locus_tag="ACG80L_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024868.1"
                     /translation="RTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYTGRENTIQIPLV
                     TAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCKIADLVGKDN
                     LIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMS
                     AKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            642..2063
                     /locus_tag="ACG80L_002687"
     CDS             642..2063
                     /locus_tag="ACG80L_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5024869.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            2099..2815
                     /locus_tag="ACG80L_002688"
     CDS             2099..2815
                     /locus_tag="ACG80L_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS5024870.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            2834..>3922
                     /locus_tag="ACG80L_002689"
     CDS             2834..>3922
                     /locus_tag="ACG80L_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFS5024871.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGK"
BASE COUNT         1471 a          468 c          706 g         1277 t
ORIGIN      
        1 agaacaaaag ctatcgcagt actcaattac ttaaaaaaaa ggactccact atataacaaa
       61 agctataatt tttattgttt ttttcctaat atatatacag gaagagagaa tacaatacaa
      121 attccattag ttacagctgg gtcaagaacc gcattgcaac tttcggatat ttttaaccct
      181 atgatagata tttataaaga aaaaaaatat atatttttta cgtctgttta tgattttgag
      241 ggcggagaac ctgtcggaga atatgaatta gtatgcaaaa tagcagactt agtaggaaaa
      301 gataatctaa ttgttaagac gcatcctaga gatgtaagaa caatttataa agataatgga
      361 tttatcgagg ataaaaattc atcaatacct tgggaggcga ttcaattgtc aggcgatttt
      421 aacgataaaa tatttatgac aattaattca ggaagtgtta tttcaggtag tacaatgtct
      481 gcaaaacctg tacgcacgta ttatatgtat aaattgtgta acatagaagg aaatccgtct
      541 tgcaaaaaga atgcgtatga tattgagcga ttgcttaagc aaaaaacgat gaaaaacatt
      601 ttaaagaatg tgaaaatagc agaaagctta gatgatatac tatgagcaaa actaaatatt
      661 taattaaaaa tatgggaata ttaacaataa gtaatttttc ttcaaaaatt ttagtttttt
      721 tgttagtccc tttatataca agtgtattat ccacaacaga atatggcaca tatgatttga
      781 caatttcaac agccacatta ttgtttccaa ttttaacctt aaacattgta gatgctgtga
      841 tgcgcttttt aatggataaa gataaaaata aaaaaaatat agcatctata gcgattaaag
      901 ttatattttt aagcgtgata gtctttggat ccgtaatgta tttgttaaat attacaaaat
      961 tatggcaaga tgtttatgga ttagaaaaat atattttttt gtattatata tcctatgtat
     1021 ggaatcaatt tctaatacaa tttgctaaag ggttagaaaa agttaaagct atgggcatag
     1081 caggtattat cagcacaatt gtaacaataa ttgccaatat tttcttcttg cttattctga
     1141 aatggggatt aattggtttt ttcattgcaa atattttatc acagattatt tcagctactt
     1201 atctgtcagt attagtgaga atatgggaat atatcgattt tgaggctaaa gatcctcagc
     1261 taaagagaga aatgctaaat tactcgattc cattaattgc aactgttgtt ggatggtgga
     1321 ttaatagtac agctgatagg tatgtggttt cctttatagt aggtgtctcg gcgaatggct
     1381 tattgtctgt ggcatataaa atacctcaaa taattaatac tttgcaggga atatttatac
     1441 aggcctggca aatttctgca attaaagaat atggagataa tgagactgca aaattttatg
     1501 gaaatacatt tgttatgatt aatttgttaa tgtgcataac ctgttcaagt cttatcatat
     1561 taactagacc gttttcacat attctttatg cgaaagactt ttatgaagcg tggaaatatg
     1621 ttccattttt attaatagct aatgttttaa attgtgcatc aggtctgtta ggacctatct
     1681 tatcagcaaa aaaagattct aaatccatga tgtggtctgc aatcataggg gcattagtta
     1741 atgtgattat gagtgtagtc cttgtttata agataggggt tcaaggtgcc acaatagcca
     1801 ctgttatatg ttcatatgta atatatgttg ttcgtaaacg agctgtggga aaagatattt
     1861 ttataaagca ttatcatatt gttgtggaat cttggggatt attgtttgtt caggcaatca
     1921 tagaaatata ttttaaaaat tatgtaatag aaattataat tatgattatt ttaattatta
     1981 taaatatcag ttatattaag cagttgtgtt atagaggaag aaacattatt tcaatgatta
     2041 agagtcgaga gcacaaacgg tagacgatgt tatttttata aaaagatata atttattaat
     2101 ggtcgataat tatgaaagga aaaatattct taaaaaaaag agtataggat gtcgtatgaa
     2161 aatagtcgct atagtaccaa tgaagttaaa taacaaaaga ttgccacaaa aaaacacgaa
     2221 agcttttacc aatggaaaac cattgtgtta ttatatactt tcaacacttt taaaagtaaa
     2281 acagattgat aaagtgtatg tctattgtag taacccagat attaaagaat ttataccaga
     2341 aggtgtaaca tttttaaaga gatctagttc cttagatcag gatacaacga gtattacaca
     2401 ggtattagcc ggtttcacaa aagaagtaga ggctgatgta tatgtaatga cacacacaac
     2461 agcaccattt attagcgaaa aatctattga aaaaggacta gatgccgtgc tgacaggaga
     2521 atatgattct gcatttgctg caaaaaaatt gcaagatttc ttatggaaag acggtgaacc
     2581 atttaattat gaattggaca atattcctag aacacaggat ttgccgcctt tatacgaaga
     2641 aactagtgga ttttatattt ataatcgcaa tgtgatgacc gagttgcata gaagaattgg
     2701 gaaaaaacct tgcatagttg aagtaggtga gattgagtca gttgatattg atgaagccga
     2761 ggattttatg attgctgatg cgatttataa tcatcttatt ttacaaggta gttaagacag
     2821 ggggaaggtt aggatgaaca atgtacagat tttagattgc acacttagag atgggggcta
     2881 ttgtaacaat tgggaatttg gatttgataa tacaaaaaaa attatttctg gacttataga
     2941 atcaggaatt gagattatag aatgtgggtt tctcacaaat aaaacagtat acaataaaaa
     3001 tgttacaaaa tatacgacct tagaagaatt agcacagata attcctgaaa aaagagaagg
     3061 aaaattattt gtagcaatga tgaactatgg cgaatataat atagaggatc ttccagatta
     3121 tgatggttca tctattgatg gaattaggat tgcgtttcat aaaaaaaatt gcaatgaagc
     3181 gttgaaatta tgtcgtgaga taaagaaaaa aggctactta gtttttgttc aagctatggt
     3241 atctctcaat tatacagatg aagagttttt agcagtaata aaaaatgtga atcagtttaa
     3301 accatacgct ttttatattg ttgatagttt tggaatgatg aaaggaaaag atttaactcg
     3361 tcttttttac atgattgagc ataatctaga aaaagatata cagataggtt ttcactcaca
     3421 taataacatg cagcttgcat attctaatgc acaaaaactt ataaccgtac aaacaaatcg
     3481 aagcctgatt attgattctt cggtgtacgg aatgggaaga ggagcaggta atcttaacac
     3541 agaactattt gtagaatatt tgaatgataa tgcaggaaaa aaatatcaat taaaaccatt
     3601 attatatata attgatgaaa tactaaatag tttctatcag aagagttatt ggggatattc
     3661 attaccaaac tatatttcag cagttcataa tgctcatcct aattatgcaa gctatctgtc
     3721 atataagaat actttaacaa ttgaagcaat gaatgaaata ttcgaaatga tggatgaaga
     3781 aaaaaaggta tcttatgata aacaatatat agaagattta tactacaaat atatggaaac
     3841 tgggaaaata caggaggaat atatggaaga actgagaaaa tttctctcag acaaaaaaat
     3901 tctactgatt gcaccaggaa aa
//
LOCUS       DAZUMB010000183         3890 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000183 DAZUMB010000000
VERSION     DAZUMB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.188"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1415
                     /locus_tag="ACG80L_002690"
     CDS             <1..1415
                     /locus_tag="ACG80L_002690"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS5024872.1"
                     /translation="KQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQA
                     KQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLND
                     VDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFS
                     VFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRN
                     GDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFL
                     LHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIP
                     TTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLA
                     TFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQ
                     TTPENEAWLQGALEVFKHEQ"
     gene            1405..2622
                     /locus_tag="ACG80L_002691"
     CDS             1405..2622
                     /locus_tag="ACG80L_002691"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5024873.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2635..>3890
                     /locus_tag="ACG80L_002692"
     CDS             2635..>3890
                     /locus_tag="ACG80L_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5024874.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1345 a          667 c          810 g         1068 t
ORIGIN      
        1 gcaaacaaac aatgttgtat cgtgaattac tttcagatga agaatcaaaa gaagatctga
       61 ttgccacaat cgttgatatt ttgggatatg caattgcacc agagtatctt ttcaacgtct
      121 tagcggatca agcaaaacaa gcaacgttcc aattgaacga tctaaacaaa gcctttgtgc
      181 aattggcatc tacctacaat caatttaatg ggttatttga tgatgtggat ctccaatcaa
      241 aaaaactagg aacagatgag caacaacgaa atgtgacgat taccgaagtc atcaagaaat
      301 tgaatgatgt agatgtttta gaacacgatg gggacgtgat cggggatgcg tatgagtttc
      361 tgatcagtca atttgcttca gaagcaggga agaaagccgg agaattttac acacctcata
      421 tggtttctga tatgatggcg caaatcgtaa cgctcgatca aaaggaacga cgattcttta
      481 gtgtttttga tccaacgatg ggttcaggtt cattgatgtt aaacgtacgt aactacttaa
      541 cacacccaga taacgttaaa tatcatgggc aagaactaaa tacaacgaca tacaatctag
      601 caaaaatgaa cttgatttta catggtgtcg acgccgaaga aatgaatctc cgcaatgggg
      661 atacgttgaa taaggactgg ccaacagatg aaccttacac atttgatgca gtagtaatga
      721 acccacctta ttcggctaat tggtcagcag atacgacttt cttagatgat tctcggttta
      781 atcgttatgg gaaattagcg ccaaaatcca aagcagattt tgcgtttctt cttcatgggt
      841 tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt accacacgga gttttattcc
      901 gtggcgctgc agaaggggtc atccgtcaaa aattactaga agatggcagt atttatgctg
      961 tgatcggtat gcctgcgaac ttgttctttg ggacatcgat tccaacgacg gtgattgtgt
     1021 tgaagaagaa tcgtcaaact cgagatgtat tattcattga tgctagtcgt gaatttgtta
     1081 aagggaaaaa tcaaaataaa ctatcggaag aaaacattca aaaaatcctc gaaacctacg
     1141 ctgaaagaaa agatgtcgag aaatacgctc atttggcaac ctttgatgaa atcaaagaaa
     1201 atgactataa cttaaatatc ccacgctatg tagatacttt cgaagaagaa gaaccaattg
     1261 atatggttca tgtcggaaat gacatcaaga agatacgaca agaacagcaa gtgctagaaa
     1321 aaaaactttt agaagctatc tcttccctgc aaacaacacc tgaaaatgaa gcgtggttac
     1381 aaggtgcgct ggaggtgttc aaacatgagc aatgatacac aaccagaaat ccgtttccca
     1441 ggatttacag atgattggga acagaggaag ttgggggact tcgttgtaga ttatgttgaa
     1501 aagacaagtg tacaaaatca atttcctatg ctaacttcat ctcaacaaaa agggatagtt
     1561 ttgcaagaag actattttgc aaatcggcaa gtaactacag aaaataatat tggttacttt
     1621 gtactaccta gaggatattt cacgttccgt agtcgaagtg ataatgatgt gtttgtgttt
     1681 aaccgtaatg acattattga tagagggatt ataagctatt tttatcctgt atttacttta
     1741 aaatctgctg attccgactt cttcttaaga cgtattaaca atggaatcca acgacaatta
     1801 tctattcaag cagagggaac aggtcaacat gttctgtctc taaaaaagtt taaaaatata
     1861 gtagcaatgt ttcctagtga ggaagaacaa caaaaaatcg gcaccttctt caaacaactt
     1921 gacgacacga tcgctcttca tcagcgtaag ttagatttgc tgaaggaaac gaaaaaagga
     1981 ttcttacaaa aaatgttccc tgaaaacgga gcaaaagtgc cagaaatccg gtttcctggt
     2041 tttacggaag attgggaaga gcgtaagttg ggagaattag gtaaaactca atcgggaatt
     2101 ggatttcctg atgccgagca aggtggttct gaaggtactc cattttttaa agtttccgac
     2161 atgaataaca ttggtaatga gtatgaaatg agaaatgcaa atcattatgt atctaatgag
     2221 caaattgagc gtaaaaaatg gaagccaata aaagatgtgc cagcgattat atttgctaaa
     2281 gttggcgcag caataatgct taatagaaaa cgtttggtca caagcccttt cttaatagat
     2341 aacaatacca tggcttattt atttaataat acttgggata tctattttgg taaaatattg
     2401 tttgaaacta taaatttgcc aagatactcc caagtagggg cattgccttc ttataacagt
     2461 tctgatattg agaatatcag tgttaaggtt cctgtaaagg atgaacaaca aaaaatcggt
     2521 actttcttca aacagctaga tgatacgatc gctcttcatc aacgtaagtt agatttacta
     2581 aaagaaacga aaaaaagctt cttacaaaag atgtttgtct aggagggaag aagaatgaaa
     2641 attagtcacc gtgaggaagc agaagtcgaa gaacagttga ttcgtgtact tggagaaggg
     2701 cataatcagt ggacgtatcg tccggatcta aaatcagaag aagacctttg ggtcaattta
     2761 cgccaaaaaa ttatctcaaa taatcaagca gaactgaatg attttccttt gaccgataaa
     2821 gaatttgaaa cgatcaaaac ggaattactg ctgcgaacga aaaccccatt tgatgctgcg
     2881 aaatggttaa aaggtgaaaa tgggatggct cggatcacga ttgaacggga agattcgcaa
     2941 ttgggttctg tctcacttat cctttattcc aatcaagaca ttggtggagg aatctctact
     3001 tatgaagtgg tccatcaaat tgcaaaacgt ggaagtaaca tagaagcacg ggatcgacgt
     3061 tttgacgtca ccttattaat caatggatta ccgattgtcc aaatcgagct gaaacaagta
     3121 actgcaaaag atggtgttta tcaggcattt aaccaaatca aaaaatatgc agaagaaggt
     3181 atgtttcgga acaatatctt ttcgacctta cagttgtttg tggtttccaa tgaacaaacc
     3241 actcggtatt ttgcaaatgc cttaccgaaa gacttgcacc ctaagttttt atttagttgg
     3301 cgaacgaaag acaatgaaaa agtagaaaac ttatatgagt tttgtaaaca agtgttaaat
     3361 atcccggatg ctcatcgttt gattgcggat tatacaatcg tcagtgaaga tcaagacaat
     3421 aaaaccctca tggtgttaca tccttatcaa gtacatgcga ttcaagcctt gtttattgcc
     3481 gcaaataaac atcaatcagg gtatgtgtgg catgcgactg gttcaggaaa gacgttgact
     3541 agttttgtat ctactaagct tttagcacga aaatcgggga tcgatcgaac gatcatgtta
     3601 gtggaccgca aagatttaga taatcaaacc accacagagt ttacaaaatt tgcttcggaa
     3661 tttaatactg ggatttcttc cgggaacgcc aaagcgaata gcttgatcgt tggaacggga
     3721 agtgccaaag aattaagtga aaccctgttg gcagatgcca atgcaaatgt tgtaatcatt
     3781 acaacccgtc aaaaactaga tgcggcgttg aaatatgcaa agaaacaaga agagaaaaaa
     3841 ggaaccaatc gcttccaaaa attaatgggc caacatatcg tgtttgtagt
//
LOCUS       DAZUMB010000184         3875 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000184 DAZUMB010000000
VERSION     DAZUMB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.189"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACG80L_002693"
     CDS             158..2257
                     /locus_tag="ACG80L_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS5024875.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACG80L_002694"
     CDS             2453..3316
                     /locus_tag="ACG80L_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS5024876.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3875
                     /locus_tag="ACG80L_002695"
     CDS             3421..>3875
                     /locus_tag="ACG80L_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5024877.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1543 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaa
//
LOCUS       DAZUMB010000185         3742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000185 DAZUMB010000000
VERSION     DAZUMB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.190"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG80L_002696"
     CDS             complement(573..1130)
                     /locus_tag="ACG80L_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024878.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG80L_002697"
     CDS             complement(1309..1920)
                     /locus_tag="ACG80L_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024879.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG80L_002698"
     CDS             complement(2033..2290)
                     /locus_tag="ACG80L_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024880.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG80L_002699"
     CDS             complement(2887..3165)
                     /locus_tag="ACG80L_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024881.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG80L_002700"
     CDS             complement(3199..3450)
                     /locus_tag="ACG80L_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024882.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1126 a          767 c          506 g         1343 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc tt
//
LOCUS       DAZUMB010000186         3732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000186 DAZUMB010000000
VERSION     DAZUMB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.191"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..454
                     /locus_tag="ACG80L_002701"
     CDS             <1..454
                     /locus_tag="ACG80L_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5024883.1"
                     /translation="KEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIK
                     GLIFLAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKII
                     IGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            447..2171
                     /locus_tag="ACG80L_002702"
     CDS             447..2171
                     /locus_tag="ACG80L_002702"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5024884.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            2168..3619
                     /locus_tag="ACG80L_002703"
     CDS             2168..3619
                     /locus_tag="ACG80L_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5024885.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         1354 a          595 c          731 g         1052 t
ORIGIN      
        1 taaagaaatc acaataaaaa gaggctggaa tatatataaa agaaattttt taagaggcaa
       61 tctgctattt agtttatttt tatctggtac tgctttattg agttacaatt tatatttatc
      121 tgttcaaatc aaagggctca tttttttagc tattgatttt attttacttt ttgccttgct
      181 ctgcctattt gctacttatc aatacagtat gattttagat agtgaatata cgatctctgt
      241 tcctaatctg ctaaagctaa gttttatttc ggttttttcc agcttttcgt cttttctaaa
      301 aataattatt ggtagcggta taattttagg cgtgacttgg caattcaaag gattgatcct
      361 gtttggtgtc attggtcttt tgacggtttg gaatggtacg atgacaacac attggcgtga
      421 agaattagat aaacagttgg aaagttatga ataagtttcg gattctattt tcaaaaggaa
      481 ccttactaaa tcgtctgttg cggaaatacg cggttatcat gatcggtgta acgatgactg
      541 ctaccgttat ttttagtatc catacttggg aacaaaatca aaaacaggca gaaaacatga
      601 caagtgatgc cgttcaaagc acaagcagaa tgctgaatga taaaacaacc ttgagtagga
      661 tcatcaaaaa ccagcttgta gggaattccg aaaagatcga aaatgtgacg acctacttaa
      721 caaaacccat agatcagtat ctgatgtatg tatacgaaca gcaaaacagt accgatgaat
      781 tagtttcttt cccaaatcaa atcaaagatc tctacgcaaa ttatgaagaa ctaagtgcga
      841 tatatatcgt gttgaatcaa ctaccggagt attatgaatc ttctagagaa aacaaaggtg
      901 gggaaataaa aaaggggaca ccaaaattac tagacgcctt ttatatcaag ttgcctatca
      961 cacaaggagg agctgagtca ggaagtattt ttattggttt tcaaaagaaa gaactagata
     1021 tgattttaga aaatctaacg tcattcaatg gactctcttt atacatgatt tctggaacga
     1081 caaatcgact atacacattc catgataata aaatagctaa agaaacgttt gaaagacaag
     1141 aaatgatcat cacagagagt ctgaagaaaa attcacaatt gccgattgac gatctggaaa
     1201 aaaacaattt tgttcaacac caagaactgt ctgatgatta tcgtatatta gctgtactag
     1261 ataaaaaaag tgtaaaaatc gaaacgggaa aaaatcttgc cccgctgatc attggtatca
     1321 ttattttaga caatatactg cttatctttc tttataaaac attcaaacga tattcacagc
     1381 aagtatctat cgtcatgcag gccatggatc aaacagcagc agggaattta gagacgcgga
     1441 tcgacataac aaacacggaa tatgaactga gggaactttc aatcggtatc aacgaaatgt
     1501 tggatagcat caatcagttt gtagaagaca tctacaaatt agaaatcaaa cagcaagacg
     1561 cacatatgcg ggcgcttcaa gcacagatca atccacactt tttatataat acattagaat
     1621 atatccgaat gtatgcaatc agcgaaggaa gtgaagaatt agcagatgtt gtgtatgcgt
     1681 tttccgcttt gcttcgaaac aatacgaatc aagagaaaac gatcacttta aaagaagaac
     1741 tggacttttg cgaaaaatat gtctatttgt atcaaatgcg ttatcctaat cgagtagcct
     1801 atcattttat gatcgatcct gatttggaaa agatcgaagt acctaaattt gtgatccagc
     1861 cattagttga aaattatttt aaacacggta ttgattttac tcgattcgat aatgcgctaa
     1921 gtgtcaaagt cttgcaggaa ggcaaacggg taaggatcat catcaaagat aatggaaaag
     1981 ggatgactga aaaacggcta aaacagattg aagaaaaact gtctcatcct aaagtggaac
     2041 ttcacggttc tatcggtctg caaaacgtga atgaacgttt acgtgccagc ttcggcagca
     2101 gttattatat gtcgcttgag aataatgaga caggtggact gaccgtttct attacattca
     2161 aggagggatg agcgtgtatc aagtgttatt ggtagatgat gaatacatga ttttgaatgg
     2221 gttaaaaaag ataattgatt ggcaaagttt aggctttcaa atagtagcaa cagcagagaa
     2281 tgccaaagaa ggactagctg tacttgaaca gcgacagatt gatttagtag taacggatgt
     2341 gacaatgcca gaaataaatg gactggaatt tattgaagcg gctcaaaaag aacgtcataa
     2401 ttttgaattt atgattttat caggatacca agaattcgat tatttgaaag gaggtatgca
     2461 attaggcgcc gtgaattatt tgatgaaacc cgtgaataag ttcgaattgg ttgaaagcct
     2521 caagaaaatc aaaacacggt tagatcagca aaatgaacaa aaaaatcaac aagaaatcta
     2581 tcaagaactg ttattcagtc aatggttgaa tgaagaattg gatgaaataa gtgaagaaga
     2641 aataatagga caaataagtg agaagcagca taggatagta ttgattcaac tggcaagaac
     2701 tcatgatact cttatcaata gatggttgca agaacggcaa caaatctttt actatcaacg
     2761 aaattacgga gaccgtgtat tgatcacgtt gttgttggag gcagatgaag ttgatggctt
     2821 ctgttgtttt gttcaagaaa actttcctga tcaggaatgg ctgatcagta tcggggaaga
     2881 gacagattct ttggacaaaa tccctgaaac attccaacaa gcgaaagaca atctacagtt
     2941 gcatcaattt tatggagata aagacgagca tgttttttac gcagtacaag cagaaacaaa
     3001 agatcaaacc atcgattttt caagtttcag acgcgtgctg caaaataaac aattggaagt
     3061 cgctcaaaaa atgatcactg atttttttga acagtttcag ttagcagcca tgatgccgga
     3121 agatatacga tattccagtt ttttactgtt tatggagatt caacgggaat tgattgatct
     3181 ggaagatgag gaatatttac aaggaatcga aaaaatccat caggcaaaaa cggtgcaaga
     3241 acttcagcag ctacttctgt cattcatcaa aaagcatcag agacagaaaa agtacagtga
     3301 caatgtggaa aaagtaatcg taatcctgca ccagcattat caagaaccac ttacactcaa
     3361 agaagtgtca gaaaacctgc atttaaatgt catgtatcta ggacagctgt tcaaaaaaga
     3421 aacgaaaaaa agtttttcag cttatttgaa ccatttacgt atggaaaaag cgaaacaact
     3481 gcttttgcac agtaatcaaa acatcaatga gatagcaagt gagattggct acaacaacac
     3541 cacgtatttc tctaaattat tcaagaaaat cgtcggcaga tcccctaaag agtaccgaga
     3601 aaacagtgga gaatcataaa agaaaaagcc aatgtaatga aataacttat aaaaaattgt
     3661 ctagaaatct aattttgggc tgttcttatt acttgaggca aggcagcttt ttcatgacct
     3721 ctcgataaac gg
//
LOCUS       DAZUMB010000187         3712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000187 DAZUMB010000000
VERSION     DAZUMB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.192"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG80L_002704"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG80L_002704"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS5024886.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACG80L_002705"
     CDS             complement(2260..2511)
                     /locus_tag="ACG80L_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS5024887.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACG80L_002706"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACG80L_002706"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS5024888.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACG80L_002707"
     CDS             3461..3679
                     /locus_tag="ACG80L_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024889.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DAZUMB010000188         3672 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000188 DAZUMB010000000
VERSION     DAZUMB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.193"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..545
                     /locus_tag="ACG80L_002708"
     CDS             75..545
                     /locus_tag="ACG80L_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS5024890.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
     gene            complement(465..683)
                     /locus_tag="ACG80L_002709"
     CDS             complement(465..683)
                     /locus_tag="ACG80L_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024891.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="ACG80L_002710"
     CDS             790..1104
                     /locus_tag="ACG80L_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024892.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="ACG80L_002711"
     CDS             1208..2386
                     /locus_tag="ACG80L_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5024893.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2567..2752)
                     /locus_tag="ACG80L_002712"
     CDS             complement(2567..2752)
                     /locus_tag="ACG80L_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024894.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            2789..3082
                     /locus_tag="ACG80L_002713"
     CDS             2789..3082
                     /locus_tag="ACG80L_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS5024895.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3316)
                     /locus_tag="ACG80L_002714"
                     /pseudo
     CDS             complement(3170..3316)
                     /locus_tag="ACG80L_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1256 a          538 c          715 g         1163 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt at
//
LOCUS       DAZUMB010000189         3595 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000189 DAZUMB010000000
VERSION     DAZUMB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.194"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..717)
                     /locus_tag="ACG80L_002715"
     CDS             complement(133..717)
                     /locus_tag="ACG80L_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5024896.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ACG80L_002716"
     CDS             complement(928..1131)
                     /locus_tag="ACG80L_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024897.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ACG80L_002717"
     CDS             complement(1141..1563)
                     /locus_tag="ACG80L_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024898.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ACG80L_002718"
     CDS             1801..2415
                     /locus_tag="ACG80L_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5024899.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ACG80L_002719"
     CDS             complement(2580..3404)
                     /locus_tag="ACG80L_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024900.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         1184 a          631 c          516 g         1264 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctcc
//
LOCUS       DAZUMB010000190         3582 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000190 DAZUMB010000000
VERSION     DAZUMB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.195"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..878)
                     /locus_tag="ACG80L_002720"
     CDS             complement(27..878)
                     /locus_tag="ACG80L_002720"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5024901.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            1001..1468
                     /locus_tag="ACG80L_002721"
     CDS             1001..1468
                     /locus_tag="ACG80L_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS5024902.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(1731..2213)
                     /locus_tag="ACG80L_002722"
     CDS             complement(1731..2213)
                     /locus_tag="ACG80L_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5024903.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2313..3176)
                     /locus_tag="ACG80L_002723"
     CDS             complement(2313..3176)
                     /locus_tag="ACG80L_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5024904.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         1043 a          771 c          565 g         1203 t
ORIGIN      
        1 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
       61 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
      121 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
      181 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
      241 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
      301 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
      361 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
      421 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
      481 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
      541 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
      601 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
      661 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
      721 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
      781 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
      841 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
      901 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
      961 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     1021 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     1081 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     1141 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     1201 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     1261 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     1321 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     1381 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     1441 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     1501 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     1561 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     1621 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     1681 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     1741 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     1801 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     1861 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     1921 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     1981 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     2041 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     2101 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     2161 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     2221 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     2281 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     2341 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     2401 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     2461 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     2521 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     2581 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     2641 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     2701 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     2761 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     2821 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     2881 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     2941 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     3001 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     3061 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     3121 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     3181 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     3241 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     3301 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     3361 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     3421 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     3481 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     3541 tatttctaac aaaatcactt catttttgtc agataaataa aa
//
LOCUS       DAZUMB010000191         3556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000191 DAZUMB010000000
VERSION     DAZUMB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.196"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG80L_002724"
     CDS             complement(<1..446)
                     /locus_tag="ACG80L_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5024905.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEE"
     gene            complement(777..1010)
                     /locus_tag="ACG80L_002725"
     CDS             complement(777..1010)
                     /locus_tag="ACG80L_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5024906.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1025..1975)
                     /locus_tag="ACG80L_002726"
     CDS             complement(1025..1975)
                     /locus_tag="ACG80L_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS5024907.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1972..2814)
                     /locus_tag="ACG80L_002727"
     CDS             complement(1972..2814)
                     /locus_tag="ACG80L_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS5024908.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2816..3535)
                     /locus_tag="ACG80L_002728"
     CDS             complement(2816..3535)
                     /locus_tag="ACG80L_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5024909.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1168 a          667 c          423 g         1298 t
ORIGIN      
        1 tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa cttatctaga
       61 caacagactg tctttttcgt cacaaatgat gtaatttttt caacaatatc ttgatatttg
      121 gttccagctg attggaataa aatcaaatcg aatatacgtt tattctcaaa aatataatta
      181 ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc tatattttct
      241 aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc attaatatgc
      301 ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata aaaagcaccc
      361 gtagttacgc ctgcggtcag gcacagttgc cttatcttag tttgctgaaa tccttctttt
      421 tcaaaatggc ttagtccact ttctattaac ttttggtgag ttataatata atctctggtt
      481 tctatgggta cttcctccta ataagatgaa ttaactttat tatcttacac aggacttgaa
      541 gtttcaatcg ttaacgttta ttactgaact cattccttag agctaaacta atgagaaaga
      601 gcagaataac tgatacaaaa agcaatccta caagaattaa tagactcatc taaatcattc
      661 accttttcta aaataatccg agaaagattt aaaaaaatta tatccagcta ttaggataag
      721 tcctatcata atcactccaa tagccatttt cttatcctcc ctaaaaataa agtcttttac
      781 ttaaaacctt ttttgtaatc gtcaataaat tttttccctt tatcagttaa ttgaaatcct
      841 atatctaaat agtcctcata tctaagttta gccagcatct cacttacaga tgctggacta
      901 acacttagaa cttttgctaa atcattattt tttaatttct tttttctttt tgataattcc
      961 tgaattgcta gtaaataaat tagtatagta gtcgttttaa tttgtaccat acctaccctc
     1021 caaattattt cgaaaggcct tcagcaattt tatccaggtt ccatttcatc atcgcatagt
     1081 aactatcacc atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt gaatagatag
     1141 gaatattggt atcttttgat actgttttca ttggtctatc atccacactg ctttctacga
     1201 ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt tgatctggtg
     1261 ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga aggcacatta tacgctttcg
     1321 agaaatattt aaagcatcct tcacttgtaa caatcatttt tttatcattc ggaattgaag
     1381 caaatttaga tttagcttct ttgtctagag aatccaattt ttcaatatac ttatctagat
     1441 tttctttata gaatttttta ttatcaggat ctttttccgc taactgtttt tcaatatttt
     1501 tagcgtaaat aataccgttt tctaaattta accaagcatg aggatcttcc ttccctttct
     1561 cactctgacc ctctaagtaa ataacatcta tgccatcact tgctgcaaaa tagtctttgt
     1621 tttcctcttt gttcgcattt ttaactaatt ttgtaaacca agcatttcct ccagtttcca
     1681 agttaacacc gttataaaaa atcaaatctg cttttgaagt tttttgaaca tcttcaggca
     1741 aaggttcata ttcgtgggga tctttcccaa taggtacgat actgtgtaaa tcaattttat
     1801 cttttgctat attttcggta atatctgcta aaatcgaatt agtaactact acttttaatt
     1861 tttcgttcga agccacttta tctttgtctt tactatttat tgaacatgcc ccgaatataa
     1921 aaagagcaat caaaatcccc agacttccca gtactgcttt ccattttctc atcataacga
     1981 tctctccttc tttcttaaaa atttttgttt tggtgagatt agaaaactaa ttacaaataa
     2041 gaatgccgct gttaaaacaa tacttgatcc tgctgctatg ttaaaactat aaccaataaa
     2101 caatccaagc aatgatgaac ttgctccgac tgttgcagaa ataaatatca tagttttcaa
     2161 gctatttgca tataaatatg ctgtagctgc tggtgtaatt aacattgcaa caattaatat
     2221 agttcccaca ctttgcatag ctgttactgc tactaaagtt aatagaacca tcaatagata
     2281 atgataaaaa ctgacattca tccctattgc ttttgccatt aatggatcaa aagaagtgat
     2341 taataattct ttaaaaaata gaactactat aaccaacact aaaaccgaaa cgccaattgt
     2401 aatccactta tcaatatctt gtacagctaa aatatttcca aataaaatat gaaataaatc
     2461 cgttgagcta tttgcaactc ctataaggat aactcctagt gctaaaaaag aactgaaagt
     2521 aataccaatt gctgtatctc cttttattac actattgctt tttataaaag taataatgat
     2581 agaagctaat agaccaaata caatcgcacc aataaaaaaa ttaataccta aaatataaga
     2641 aattgctact cctggcaaaa cagcatgaga aatagcatca cccataagag acattcctct
     2701 taaaataata aaacaaccta ctgttccaga aacaatccct ataactacag atgtaattag
     2761 agcattctgt aaaaagtgaa agtcatacag accattaata aaattttgaa gcatattatt
     2821 ccccctctcc aataaatata gtatctccat aggctttttt taaattttct tcattaaata
     2881 cagattctac acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat
     2941 acttctttac cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca
     3001 atttttttaa cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt
     3061 catctaaaaa tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa
     3121 attgtcctcc agataattct cctatttgat gattagaata ttttcccata tcgacctttt
     3181 ccaaagcctt tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc
     3241 cggcatatgt tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat
     3301 cagctttttg ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa
     3361 tggagacctc tccctgatga gggataatat ttaacattgc tttaagcaat gtggattttc
     3421 cagcaccatt tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct
     3481 cgttcaatgc taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt
     3541 tctccttcta aatgat
//
LOCUS       DAZUMB010000192         3405 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000192 DAZUMB010000000
VERSION     DAZUMB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.197"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..1963
                     /locus_tag="ACG80L_002729"
     CDS             197..1963
                     /locus_tag="ACG80L_002729"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS5024910.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2065..3324
                     /locus_tag="ACG80L_002730"
     CDS             2065..3324
                     /locus_tag="ACG80L_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5024911.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
BASE COUNT         1219 a          552 c          723 g          911 t
ORIGIN      
        1 gataaattca ggacactaaa tagtacagga agtagactaa aacttttaaa agttttaagt
       61 ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt taccgataac
      121 atttttgaaa gcgttgtctt ttgcttctct atcacttata gtgatgacgt catattaaaa
      181 cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa gtgaatatgc
      241 atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa atgatatcaa
      301 aaaagtatat gtgctatgtg gagatccata taccataaca actgaaaaat ggtatcaaaa
      361 acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg aaattgaagt
      421 gacgagcgaa acaagtcgct tacagtatgc gttccatgtt gttggggaag ataacacgga
      481 ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta ctgaaccaaa
      541 tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc cagaatgggt
      601 aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg acaaagcaaa
      661 tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag actttttcgg
      721 gggagatttg caaggtgtga tggatcattt ggattacttg acggaccttg gcatcaatgg
      781 catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata cgattgatta
      841 ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga ttgatgaagc
      901 acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg gtgtccattc
      961 accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag actggttcca
     1021 catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa ctgctgaaaa
     1081 tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata cagcaaatcc
     1141 agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat tcgatattga
     1201 tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa aattcagaga
     1261 agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc attcctctca
     1321 agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattta cagattcgat
     1381 taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg gaatgaatca
     1441 tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat tagattctca
     1501 tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga agtctgtgat
     1561 ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag aaatagggat
     1621 gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg aggaacaaga
     1681 tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt tgtctaagat
     1741 tatttctgaa ggttctactc agtggctaat agtaaatgat cgtgaagata agctctattt
     1801 cacacggaaa ctagagggac agatcatcta tgtttacttt aatcagtcaa aagaaccttg
     1861 ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt tagaagacgg
     1921 gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt taccggtaac
     1981 aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg taacagcacg
     2041 ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt gggtttaggg
     2101 ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa ctcttcagca
     2161 gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg atataaagat
     2221 tatataaacg aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat caagctagta
     2281 gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc tggaaaggga
     2341 cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca aggacatctg
     2401 gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc ccaagtgact
     2461 tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt gtactataac
     2521 aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct atccaaagac
     2581 agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc taaatggact
     2641 gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt tggtgatgat
     2701 ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg aatctcttat
     2761 gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa aagtgcagga
     2821 gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga cggtccatgg
     2881 caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat tcctactcta
     2941 aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt cagcaattat
     3001 gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca ggaaaatcaa
     3061 gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag ggaaacagca
     3121 aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac agctcaagcg
     3181 atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt gatgttcgat
     3241 gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa aacaatcagt
     3301 gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat aaaaaatgga
     3361 acaggtgctt cagataaaag tggcaagagc gagatggaaa attat
//
LOCUS       DAZUMB010000193         3332 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000193 DAZUMB010000000
VERSION     DAZUMB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.198"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..282
                     /locus_tag="ACG80L_002731"
     CDS             <1..282
                     /locus_tag="ACG80L_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024912.1"
                     /translation="EELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSE
                     LVSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            296..985
                     /locus_tag="ACG80L_002732"
     CDS             296..985
                     /locus_tag="ACG80L_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024913.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1110..>3332
                     /locus_tag="ACG80L_002733"
     CDS             1110..>3332
                     /locus_tag="ACG80L_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS5024914.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     D"
BASE COUNT         1112 a          445 c          714 g         1061 t
ORIGIN      
        1 gaagaattgt ataaagaatt tggtgattta tccggggaac agttagaaga aagtattcca
       61 ggaattagtg actcagataa aattgttgtc ttgtctgctg gtgggcaatt aatgggagat
      121 ggcaaatccg aattagttag ttataaacaa aatcacagac agtttattga tgttaatact
      181 gattccaacg ctatttcgat taaggatcta aaagagcttt taaatagtaa aagaaatgaa
      241 ttaaagcaga taaaagaaac taattcagtg gaattagagt aaaggaagat tcattatgaa
      301 aaagattttt ggtgtttctg ttgggctact tatgatgtta acatttggaa atgcagtata
      361 tgctgctgag aaagaagtta tttcagatcc agagcagtac gcctcacgat actttgctga
      421 ttttgaagaa gaagataaag tacttgcatt gccagaaggt ggttttttaa aaggggaagc
      481 taaaattgta tctatggata atccagatat aattcaaggt tcatataatt cagaaactga
      541 tcctgcttca gtttcagtga cagaagcaaa aaaaattgtt gctgaaaaat tcaaaaatga
      601 tattgaaact actccaatta tgcctcgagg agcatttcca cccgatgttg gtactagcgg
      661 acaccttctt gtactagcag gaggaggaag ctataaatca cagccattta gtggtgcggg
      721 atggcgtttt ggaggtatgg gtgtaaaacc agctgatggt acaggcaact ttttacgttg
      781 gaaatcctac attgacgatg gaagagtagg aaatgtaaat caagccaacg caactaaatc
      841 tggttctatt caaggaatgg cattgtatgc tcgtgaagga ttcaaatact ttgcaccaaa
      901 cgggcctggt tggacaatga atataggtat ttattacact tataatcctg ttgcaggtac
      961 gtattatgtt gttcaaaata tttaataaaa cagcacaaag agatttcttt gtgctgtttt
     1021 attattttta gttgttgaca aacactaaaa tataaaatat aatcaacaca taaacgaacg
     1081 tatgttcggc aagaaagcag gggattttta tggatgagat ggttttaagt actcagaaat
     1141 ggctaaacaa gaaatacagt aatgttacag ggtttgataa ggttccagaa aatgggcgaa
     1201 cagggtggcc tacaatctat ggacttattg aaggactaca agtagaactt ggtattacta
     1261 atctagtagc taattttgga cctaccactg aaaaaatgta cgataatcag gttactccta
     1321 aatggggcaa aaatctacct aaaaatattg tatttcttat tcaaggtgcc ttttggtgta
     1381 aggggattaa tccaggtggc ttcgatgggg tatatactcc atatttagat actgctgtaa
     1441 aagagttgca gactgatgca gggtttaatg gaagcgctgt aactggagtt ttggatgcga
     1501 aatgggccaa agcgttattt gatatgagtg cttttgttct tgttcccggc ggagatagta
     1561 aaattagaaa aatgcagcaa tggttaaatg taaattattt agaatacacg ggaataatgc
     1621 cttgtgatgg tatttatcaa agaaattcaa atcaagcatt aatttttgca ttacaagctg
     1681 aactaggata ttctcccagt gaagcaacag ggtcttatgg taatgggaca actagtaaaa
     1741 cttatcctgt gtctgaaggt aactcgggaa actatgtaag aataattcaa tatggattat
     1801 atgttaatgg ttattttgaa gacggtacat tcgatggtat tttcactaaa tatatgggtt
     1861 tagaagtact ggcattccga aaatttatgg tattgcctga atatactgaa atagcagatg
     1921 ttgttgtaat aaaaggacta ttgtctagcg ctggagatat tagaagatct gcagatggcg
     1981 cagatacttc tacgcaatta actagatctc aaatccaaac attggttgat aatgatataa
     2041 aaatagttgg tagatatctt actggtactg ttggaattgg aaaagatgaa cgaaataagt
     2101 atctaactac agaggaattg aataatattt tttctaaaaa tttatctgtg tttcctatct
     2161 atcaagatgg aggtgcagca ttagcttatt ttacttatga tcgtggctta tcagatggta
     2221 agaaggctat agatgcagca aaaaatctaa atataccatt aacaacagtt atttattttg
     2281 ctgttgatct agacatgtta ggggaagaaa ttcttgcata tgctggagaa tattttaagg
     2341 gggtatctgc tgtaatgtca tatagcgggt atcaaacggg tgtttacgga acaagaaatg
     2401 tttgttctca aataatcaat aacgggtatg cttcgtttgc atttgtttca gatatgtcga
     2461 ctggttatag tgggaattta ggttttccta tgcctagaca atggtctttt gatcaatttg
     2521 ttgaatttac cataggaagt ggcaacggag ctgttggtat cgatttaatc gctacatcgg
     2581 gtagagatag cggatttaat gagttatcaa atgatactaa taatgataat tacattgcca
     2641 aatacaatca gaaggttata aatcagatgg tacgtgcgta tcaatatcta tcacaagctg
     2701 gtcttgataa tccttggaac cctcatttga cattttatag atatgtaaat tattctggtt
     2761 tgtcatggga catcatttct tctcctgtaa cagagcatga tagaaaaatt tatgatgaat
     2821 ataggaataa attaactaat agcgagggcc tgtataatta ttttatagat cctaatactg
     2881 gttcaataat aggtttgcct cacttaattg tgacgttaca atcacagatg tttctagccg
     2941 acacaatcaa tgatagtgtt tctgactttg caggttggtt aggagattta atgacttgct
     3001 ggggtgaagt taagaaatta ggaatacaaa tggagcaagg tgtatttgca ttagtaggta
     3061 cggctgcagg tggaacattt agtctagaag atttatacca agatgttgac gcggtaaatg
     3121 tttatacctt agcaaaaaga aattctgatt caacagcttt atcggcttta aaaacgtact
     3181 attgtgataa gggtgctatt gatagattta atcaatttgt taagaacaga tttggttctt
     3241 cagataaaat cgcttcagta acaaatagtt tattagaaaa tgacgaaaca gttgatatct
     3301 taggtgtagg gataatttca tttttcattg at
//
LOCUS       DAZUMB010000194         3320 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000194 DAZUMB010000000
VERSION     DAZUMB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.199"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG80L_002734"
     CDS             complement(<1..2232)
                     /locus_tag="ACG80L_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5024915.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG80L_002735"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG80L_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          910 a          744 c          535 g         1131 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa
//
LOCUS       DAZUMB010000195         3304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000195 DAZUMB010000000
VERSION     DAZUMB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.200"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1074
                     /locus_tag="ACG80L_002736"
     CDS             202..1074
                     /locus_tag="ACG80L_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5024916.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1071..3029
                     /gene="nagE"
                     /locus_tag="ACG80L_002737"
     CDS             1071..3029
                     /gene="nagE"
                     /locus_tag="ACG80L_002737"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS5024917.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
BASE COUNT         1019 a          645 c          690 g          950 t
ORIGIN      
        1 aaaagaattg acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt
       61 tcatgtgact agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag
      121 gtttttcgtg tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa
      181 atcttcaaaa agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg
      241 gtagatgacg gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa
      301 agaaatgatt tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt
      361 caaatcaagt tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa
      421 acgatcattg atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc
      481 gctttgaccg accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc
      541 aacaaacttt taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg
      601 gcagtagagt atctgacaaa agagcttggg attccatata cttatgacga agcaggatat
      661 atcgcaatcc atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt
      721 gaagtgacga ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc
      781 cattcagaac cattttcact gaattattca agactggtga atcatttacg actgcttcta
      841 caacgaaccc atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa
      901 cgaaaatacc cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa
      961 taccagctcg cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta
     1021 agaagcgcaa tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat
     1081 atctacaacg aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt
     1141 tactagtagg gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt
     1201 tacaagctgg cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag
     1261 cactagggat gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg
     1321 aagttccagt aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag
     1381 aatcagttga tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat
     1441 tgattgcggc agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt
     1501 tctttagcgg caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg
     1561 cagtgctact gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca
     1621 ttttgagttt aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc
     1681 ctacaggatt gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg
     1741 atattggtaa atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg
     1801 gatttttccc aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg
     1861 cacgtccaga aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat
     1921 tctttactgg tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat
     1981 atgtcttgca tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga
     2041 cagcaggatt tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga
     2101 ttgcgaatca gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact
     2161 ttggctttaa ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag
     2221 atctttcaga tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa
     2281 gcaaaatcta cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta
     2341 cacgccttcg tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca
     2401 ctggcgtacc tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag
     2461 aagtacaatt cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg
     2521 cacaaacaaa caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata
     2581 tttacgctat cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg
     2641 cggaaaaaat gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc
     2701 caatcgctgg tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag
     2761 atgctggtat cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac
     2821 cattcacttt atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag
     2881 ttgatcttgc cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa
     2941 atggcgacaa agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata
     3001 aaatcggctc tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat
     3061 aaacaaacag gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc
     3121 gttctcctgc caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt
     3181 tttatttgac tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg
     3241 gtgttcaaaa accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat
     3301 tttc
//
LOCUS       DAZUMB010000196         3252 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000196 DAZUMB010000000
VERSION     DAZUMB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.201"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            648..980
                     /gene="traE"
                     /locus_tag="ACG80L_002738"
     CDS             648..980
                     /gene="traE"
                     /locus_tag="ACG80L_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS5024918.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1599..1940
                     /locus_tag="ACG80L_002739"
     CDS             1599..1940
                     /locus_tag="ACG80L_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS5024919.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(2177..>3252)
                     /locus_tag="ACG80L_002740"
     CDS             complement(2177..>3252)
                     /locus_tag="ACG80L_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5024920.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1049 a          484 c          666 g         1053 t
ORIGIN      
        1 aaataattaa tttcaaatat agaaaaggga acgtatgttt gcatactcct tttttttgag
       61 ttataataat acgcgaagag agataatttc attatttaaa caagctaaaa atcttgatat
      121 tacttctttt caggcaatac gaatattatt cgcttacatt ttttgtataa atttaattgt
      181 ctgctatgct tgtgatgaat tgattttcgt tctgcacttt tggatgataa atttggacaa
      241 ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac cttcgtcagc
      301 gttcatacac ggcattaaga tttggccctt aatgcgttaa cctttcagtg ttacggtatt
      361 acccgcaatg gatttagtat aacagatatt caaaagtgat accacttttt ttggctgtgt
      421 taaggttaaa aatacgtgat attacgatga atctatgcct aagcacaatg ttataaaaat
      481 gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta gagggaacgc atgtttggat
      541 gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt ctctattttt gaaaaataac
      601 aagaaaaact tgttaaaaat atttaaagaa attgggtggt ttattttatg tcatatacag
      661 gaattttgag tctcaaagat atttgtcact acggtaaacg gtgtaccgct acagagaaaa
      721 tcacgaagaa actatcaact ggccaaagta aaaccgttgt gcagtgtaag aaatatataa
      781 tccagaaaga caaagtctca gaggaaatga tttattacat tgggaaacaa aagcaaataa
      841 ttttaaaaga tcctatcccc ttaaaagagc tatatccaac aatcaaacat gtttatgacc
      901 aaaacggtgt actgattggt cgaagaaaaa atggagtctt gcgttgtacc gcaaaaggga
      961 tggggcgttt gatcggctga attgatcatt ctcataactt gtattttatc gtttttcgct
     1021 tgcttgaaaa tattgtcgag acttctaaag gaacttagag tcactagaaa aataaggagt
     1081 agacttgatc tatcaacaat tcccttcggg gagtgacggg gtaggtttta acgattggta
     1141 acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga aatgaatttg
     1201 tgtaaataat aagcgcagag taaacaagag ggaatttaga agttagacaa gttagaatag
     1261 cgtctagatt aatcatgagc agctactaag ggatctagac tttgacgcaa actcatggaa
     1321 taaggagtag gtcttctcgt aatggggagg acgcacaaga aattgtgtcg tgtgctgcag
     1381 tacatgtaga tacttgcgag gacgttgatt attggatcaa tcgagcattt accgctgtct
     1441 actagggttt ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt atttttaagt
     1501 aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt gatataaaac
     1561 atagagaaaa tgaaatttac gtaatggagg aatgacaaat gaaaaattat gatccgaata
     1621 tacgattggg cacacatact attaaggttt ctttccaacg ttgggattac aaaggatttg
     1681 tgacgtttag aagaggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg
     1741 atttatacga tcaaacattg actgataatc caattggatt cggcttgcta cctgaagatg
     1801 atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga taatggagac gaattatctg
     1861 ttgaagatac ttggtcatat ttgagtgatt atattgtcag ctttgaaatc attgaatttg
     1921 tagcggataa agaagaataa aaactggagg aataaatttt acagataaag gcttagaagg
     1981 agtcacgatg attaataaaa aggtagtatt accttaggca ttgagtaagg aatttattta
     2041 gtctaatggt gataagtcaa gatgaaattt aagattttct ttattgagta atatgttttg
     2101 aagcgcacga aactagaccc gatcaaaatc aaattttttt cgggttgatt caaggatttg
     2161 cgtcaataac ggattgttat ttctctatca cactccttgg tggcgtatag agttggtgtt
     2221 tacgtagtag cgtatccacc agacgcgtaa attttcttgc ggttaggacg agtgcacgtt
     2281 tgtgttgatg ctttggaacc tccttatatt tactttgata aaaagctttg tattcaggaa
     2341 gataattttt tacagagttg gcagcttcaa ctaagtagta tctaaaatat cgatttcctt
     2401 gttttgtcag aggtgtattt tgcgactgat agtttccaga ttggttcact ttccaactta
     2461 atccagcgta ttttgccaat tttgtctgat cttcaaatct ttcgatttga ccaatttcag
     2521 cgattaaacc ggcagcatag actttaccaa cgcctggaat actggttaag cattgatatt
     2581 ctggaatgac aacaattact tgttcgatgg ctttatctaa ttctttgata tttttttcaa
     2641 gtgctcgtat ttctcgtacg agaacactta aaacaacatt aattgattct tgtgcaagag
     2701 cacctaggcg gtaactcatg gtaattgcac gttgaattgc tttagcgatt ttttctggtt
     2761 gcttgaaacg gccttttcct ttttcttgaa gtaaatcaca gaaagcttct aaaggaagct
     2821 cagcaagttc atccaatgtg aaatcttccg tcatgagtga aatgagggtt gcactaaaaa
     2881 gactcgtcga ttcgtctcgc atttctcgag caagcgtatt acatttgtac gttagatttt
     2941 caagaaaatg ttgctttgta cgaatcaatt gcttaataag ttgatagcga gtacgtgtta
     3001 aacgctgtaa ggccatgtat ttctcttctt tgatagggga agttgtgaat cgttgaatac
     3061 gcaagaaatc agcgattctt aatgcatcgt ttcgatcagt tttattctca tcaaacatgc
     3121 gactaaattg tttcacccga aaaggatttt caatcgttac aagtgtgttg agcttcttta
     3181 gtttctcatc ttcatgaaaa aacatggaag gatggaagct gtacatggac gttgactcca
     3241 tgccaatcac aa
//
LOCUS       DAZUMB010000197         3250 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000197 DAZUMB010000000
VERSION     DAZUMB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.202"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..680)
                     /locus_tag="ACG80L_002741"
     CDS             complement(<1..680)
                     /locus_tag="ACG80L_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024921.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFA"
     gene            complement(867..1112)
                     /locus_tag="ACG80L_002742"
     CDS             complement(867..1112)
                     /locus_tag="ACG80L_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024922.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1129..1980)
                     /locus_tag="ACG80L_002743"
     CDS             complement(1129..1980)
                     /locus_tag="ACG80L_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5024923.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2146..3150
                     /locus_tag="ACG80L_002744"
     CDS             2146..3150
                     /locus_tag="ACG80L_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS5024924.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1008 a          564 c          631 g         1047 t
ORIGIN      
        1 gcaaattgtt cgatcaaagg aatcaaagtc aatttgactt tgtatttccg ttcgagtgct
       61 ttttcgataa cgaactcatt ttcagtagtc atagaaatag ttgctaattt acgaggttca
      121 tcagcatttt tttcattaat aactacttca tgttcatttt gttctgtgta aaatttagga
      181 tactcagcaa cggcttttga aaaatcagca aaacttaaag cggcattcgt atcagttgag
      241 cgtgtatttt cagttgtgtc cgcttttggt ttttccgttt tttcttctgg cacactgact
      301 gtttcttctg taactggtgt cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt
      361 tgatcattcc agtaagacac gatttcaata agaagattgt cacggaatcg ttcgaaaatc
      421 ccgttttctc ctgaacgaat tctctttaat acagtattga agttgtcatc gattggcacg
      481 ttgatccgtg tatcttcatt tgttttccca gctaatccga tcgttaagat ttgacggttt
      541 ttgggatcaa gcatgacttg taacggcaaa ccataacgtc gcaagctctc ttcttctaga
      601 gaacggaata attgtcctct tacaccatcc aatgaaggac ggaattgatt taataatttt
      661 tgtttctgtt ttttcttcat atgttctctc cttcgctcat accctttcga ttataagaaa
      721 aaaacagcca aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa attacaacag
      781 aaaaatttgc gtattttccc gttttttgaa agaaaaataa aaaatggaac ggattatgta
      841 tatatacata atccgttcca ttctttctat tttttcggta tcaaggcaaa aacaggtagt
      901 tcaggtgaat aaatgacttc atgttcgtgc cagggtttga ccattagcga atgatcgaac
      961 cgttttttca gttcttcgat gatttgatca tgtgtccagt ttcttgcagg aaaatgttgg
     1021 attttctctg catcgatcaa aaagacaaaa tcggaatgaa aagtctgggt aacttcttcc
     1081 gagagagaat tcaagcgttc gattggatcc ataaaatcac ctttcgactt aaaattgggt
     1141 agtatctggg tcttttgctt tgccgactac accatctaac tgatcgattt gtttcatttc
     1201 ttcttctgtt aattcaaaat caaataattc agtattttct ttgattctgc ttggtgtcac
     1261 tgacttagga agaggaagga attcgtgttg cagcgaccaa cgcaaagcaa cttgtgcaat
     1321 cgtcttgtca tgtgcttcag cgatttgttt catttcaggc acatcgaaga ttttaccagt
     1381 gcctaatgga ctatatgctt cgaggatcat atcatgtttt tttgcatact caactacagt
     1441 tgtctgtaat tcgcctggtg caaggaaaac ttgattgacc atcggcataa tttttgctgt
     1501 ttttgctagt gtatctagat ggtggggtaa gaaattgctc acaccgattg ctttgatttt
     1561 acctgcttca tacagttctt caaatgctcg ccatgtatct gcatttgctt gttcccaatt
     1621 atcacgaaaa gcaacagggt ttggccagtg gatcaaaaac agatctaaat aatctgtttg
     1681 gagcttcttc aatgattctt caaaagaaga catcacaagt tcatagctgt ggtttgcatt
     1741 ccataatttt gttgtcaaga atatctcttc tctaggaatt ccactttcct taatcgcttg
     1801 gccgacgctt tcttcattct tgtatccttg ggcagtatcg atatggcgat aacctgcagc
     1861 cagcgcttct ttaacagagg aaacagcgac atccccatca ggtgtttgcc atgtgccgaa
     1921 tcctactacc gggatttcgt aaccatttga tagacgataa gtgcttgtta atgaattcat
     1981 actttcagct ccttttttat tcgttcaact ataagtgtag cgcatttttc gaaaaaaaga
     2041 atccattttg tttctactct tgaaattttt gtttaaaaga aaatgatttt gattctggta
     2101 tttttgattt tttttccgta aactaagaca gaagggaatg agaaaatgga tatctattta
     2161 aaaaaagcaa ttctgcatat tattgacaga gaaactggtt ctccagtctt ttcacaaaaa
     2221 gagctggatc tgacaaaaga atatattcgt gattttcttc aaaagaagat ccaaaaaatc
     2281 tcttctgctc aaacaaaaac tggacaacta gcagaagaaa gcatgatcgt tgaatcactt
     2341 aaaatcgctc aagaaaattt tgtggaagca agtgaagcaa tcgttcagcg gtggttcgat
     2401 atctaccaag aaagtgaaga agcacctagt gcagatgtat ttgttgtctt gtatgaatta
     2461 gatacagtga tgtacttatc tttattgaaa gtcaattatc gtgaggctta cactcacttt
     2521 gttgaagcag acgaagcagg gatcgacaac cgcttgattt tgaatcgagc aattctaggg
     2581 gcgaaaacac aaaaagcaga tgaagcattc gctgtgaatt tgactgattg gacctacgaa
     2641 ttaattgaaa aaaaatatga gttttctggc aaaaaagagc tttatttttc tggaagagtg
     2701 atcgaaagtg ttcctgcacc ttctttagaa gaaaatgtaa aagtgatcca aaaagtagcc
     2761 aagcatcttg gaaaaaaatt tgagacagaa gaatttgata tcatggctga tttgaaagaa
     2821 gctgtctatg atacgataga agaaaaaggt cggttggatc atgagatgat tgcggaaaaa
     2881 gtcttcaagg aaaacatcac ggcaaaaatg gcttttaaag aagaagtaca agagcaagga
     2941 tttgtcccag aagcacctcc tgtaaaagaa gtacaagaaa tttctgagaa aaaatttgga
     3001 aaacaaaaat tcaagctatc aaacgggatc gaactgatcg taccagtaga tgtatatcgc
     3061 aacccagatt tgattgaatt tgtgaataat ccggatggta cgatatccgt gatgatcaaa
     3121 aatgtggatg aagtattgaa tcgtttataa aaatgactag aatattaggt gttcaagtac
     3181 cagtgatcag ttaaatgaat tgtgtcaaga gaaggatcgg aaaagacgaa tataacaaaa
     3241 aaagagcgtg
//
LOCUS       DAZUMB010000198         3221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000198 DAZUMB010000000
VERSION     DAZUMB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.203"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG80L_002745"
     CDS             301..585
                     /locus_tag="ACG80L_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024925.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG80L_002746"
     CDS             588..1004
                     /locus_tag="ACG80L_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024926.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG80L_002747"
     CDS             complement(1141..1860)
                     /locus_tag="ACG80L_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5024927.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACG80L_002748"
     CDS             2544..2630
                     /locus_tag="ACG80L_002748"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5024928.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACG80L_002749"
     CDS             complement(2932..>3221)
                     /locus_tag="ACG80L_002749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024929.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZUMB010000199         3184 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000199 DAZUMB010000000
VERSION     DAZUMB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.204"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(674..2128)
                     /locus_tag="ACG80L_002750"
     CDS             complement(674..2128)
                     /locus_tag="ACG80L_002750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024930.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(2129..2428)
                     /locus_tag="ACG80L_002751"
     CDS             complement(2129..2428)
                     /locus_tag="ACG80L_002751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024931.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2731..3102)
                     /locus_tag="ACG80L_002752"
     CDS             complement(2731..3102)
                     /locus_tag="ACG80L_002752"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5024932.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT          960 a          696 c          526 g         1002 t
ORIGIN      
        1 atactctata atctgtttat ttgcagactg ataatagcaa aagaaaactt ataatcaaat
       61 aatatcaaca tttgatcaca taatttgaga ttgaatgctg atttacctat gttttacgta
      121 atggtcttta gttatttttg ctcataaaat gcataaaaca cgcgttttta tgtgtgcttg
      181 aaatttcttg gctctataat ttctaagact gataaaaata ttctatcggt tctttttgca
      241 aataaaaaaa catatagaag ctccattaga ttgaatcacc acaacacaat caaaggagca
      301 ttccgtatgc ttgattttca ttctataaaa ctacttttag gaatcaaaga aaatcacaac
      361 ggttcaaact aaaagcaaga atatccgaag taccattgtt tcatcgatca tgagaaaagc
      421 attcacctct gtctgcagat gttcttatat cgctctgtca gtcctattaa taaagtactg
      481 ttttgttttc aaaaaaacta ttacgagtac tttttaagag aatgccttgc aaatattcat
      541 cacacaccgt tatataagtc aaatagtttc tttcagtcgt caagaaaaga ttctcctgtc
      601 cttggcatta tcagttaagt agcaacttaa ttctcggact tgttctactc tagcaaaatt
      661 tattttcatc aacttacgtc caaatgtttt tttgaataat tacttcatcg cgggtctgga
      721 agacttgcct cattcgataa gtcactagct tttggcgtcc ttcaggattt ctagtataca
      781 ccaactgaca tttgggcagc gacgattgat ttaattcgtt tagaaaaagg ttagcgattt
      841 gcttatttcg gccaaagcgt tctggtaccg tcagaactat ctttttccct tcgattaaat
      901 accgcggatt ctccagcggt gctaaaagct ccctcatagc ttcagtaaaa agctgtcctt
      961 ctcgatagcc agcattctca agatagcatc taaattcctg ctgatcagcc gctacgatta
     1021 cttttctatc actaacttca ccaaggcgct gcaacgtcgt ttgaacgcat atggccaagc
     1081 tttttttgcg ggcacattcc ttttcccata gcttttgtcg cctacgaatt tggaaatatt
     1141 ccagcaagtt acctttaaat atagctgacc agaagcttag aggctctata ttttttacta
     1201 ctatcggcaa tgaagtttct tgtttttgct ttacctgatc gactttccta acgacgattg
     1261 gcatagatag gtgccagcca tcagctaaag cagtactcca ttgttctttc aaccgacttc
     1321 gttcacaaga aaaatagaac atcgactgag tataagtttc catcgtggtg gtcgtatatt
     1381 ccagtggtaa agcaaagcga tcgccaccac tctcaatttt tggtgaagat tcaaaggtca
     1441 gacctgaaat attgcccaaa cgccgtagca ttcttttcaa atcaggctgt tcagacagag
     1501 gaactcccgg taatccaatc gctaaatgcc aaatgttcgt tactttattt tgtgcaccta
     1561 ggcgtaagcc ccgccctcgt agttgttgtg tttgcacaaa agtggaagct tgattagcca
     1621 agatcaacag attaattgca ggacaatccc agccttctcc tagcaaacta acggttccta
     1681 ccaaaatatt caacttcttt tcggttaata ggcgagtaac tgcttgcatc aatgcttggc
     1741 ggtttttgtc atccaccgtc aaataaaaat aacccgacat ataaggatgt tcattcatac
     1801 ccgtcgctag ttctgccagc tcaacagtta ctagcatttt cggtaacagt aagaactctc
     1861 cacagatggc accgatttgc tgagtgttac ctaatagacg aacaacttca tcaaacaaag
     1921 gaaccacgcc ataagctaag gtaccatcac tgcctgctaa tacctccttg caaatacgat
     1981 ccatcaaaat gaccgctgtc agttcttgct tcagctgaac ttcttctttt aacaaaattt
     2041 gacaaactgc ttgctttttc tcaggaatcg cctgactacc aacagtgtct ggatactctg
     2101 gaaacaaatg cagcgactca tcaaacaatc atccagcttt tttcaattga taaaaaatag
     2161 gcaattccgg ttgatcttca aacatctgct gataaatagt gatcagctct tttttaccta
     2221 aagatggaac tgaagctttt ttaccttgct gcttagaact gcctaatagc tgccaatgtt
     2281 cgggtatcaa ttcatagtta tgatgattca agtaaattaa tgcactagtg aacagctcaa
     2341 aattttcata aatttccttc gaatgctcaa gcggatgctg aacaaattct aaagaaagca
     2401 aataatccac cgtctcttgg ctttccatta acgcttccac cagctcattt tgtttctcta
     2461 aaaaggtaaa ataatgctga gtagtttcct gattagccgg aatcaaatat agaaaatcct
     2521 gatgaggaca tagaacctcc tcctttacca actcgggaat gctaatttct tcgtcaatca
     2581 ggccatttta actcaatata ttgcttccac tccgtctgag agctatcaat cggaggcgtg
     2641 gctgtcaagg aaatcgtcaa cagattcggt tgttttttct tcaagtcaat caacaattca
     2701 ccccagcttt tttttaaatg atgagcttca tcaaaaaact aacagctcca gctgatcacc
     2761 ataagttggc tcctccttca gtcggttgta aatggtctga tacgtttcca ctgtaatatg
     2821 actaggatgt tcaatcgccc cagaaagata gcaactatct gctggattta ctaagaaata
     2881 ttcctcgatt gttgttaacc actgtttctt caatattagg gaaggaacca aaattaaggt
     2941 tttacaatcc aaacgtcgga tcatctcaat tcctaaaaag gtcttgccag cccctggtgg
     3001 agccactaga tggactgaat tgttatttaa ataagtagca aagttagtca atatacgctt
     3061 ctgatagcta cgccactgaa ttttttcatc agatagccac atcccaggtc cccctctcac
     3121 gtattttctc tatcataatc taaatgaata cagcaaaaaa aagatttctt caagaatatg
     3181 tgtt
//
LOCUS       DAZUMB010000200         3091 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000200 DAZUMB010000000
VERSION     DAZUMB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.205"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2006
                     /locus_tag="ACG80L_002753"
     CDS             <1..2006
                     /locus_tag="ACG80L_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS5024933.1"
                     /translation="FFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNSESQT
                     ILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDYEFYY
                     TSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDAL
                     RDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENIN
                     RQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALK
                     IVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEV
                     SEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFS
                     KGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKC
                     AIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLD
                     VNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIE
                     LGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKT
                     SDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2003..>3091
                     /locus_tag="ACG80L_002754"
     CDS             2003..>3091
                     /locus_tag="ACG80L_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5024934.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELP"
BASE COUNT         1166 a          420 c          523 g          982 t
ORIGIN      
        1 atttttttca tggaattcaa aatgggtggc aagggcattg gattattatc tctgcgacat
       61 ttgaaggaat cagcgaagaa gaatttgaca atgagatttg tgcttccatt tctttaaatt
      121 ctgaaagtca aacaatctta gaagaattga tttcaaatgc agataaaggt gtaggtagta
      181 tttctctatt tattagacct aacaaagcta ttagaaagca attatttgat atttccactg
      241 aattagactc tcatcaattt aacgaattta gaaattctat tcgtctatcc gactatgaat
      301 tttactacac atctaaatca aatctaaatt tctgtatcaa tgaaaattat gataaattgg
      361 taggaagact caatgacaat atagctagta atcctgaaga agacgatgaa gctctgttgg
      421 gctcaagaat tgacatgtca gatattttca agcatatttc ggttgtttat gttgacgctc
      481 ttcgagatgt tcttagagaa atgaaaaaca atcgaaaccc agtcaaaaga atcatggaaa
      541 caattgaatc aaaaatatca tcagataatg ttgagtcatt aaaaacaatt atacagcaat
      601 taaatgagac tattaccagt gttcctgaaa ttaggaaaat tggcgaaaat attaatcggc
      661 aactaaatag cataattggt tcagtgtatt ctcctaatct tcttctttcc tcaactatgt
      721 cagatgatat gggttcttta gcaaaatttc tttcaatgaa gccagaacaa aatatcgact
      781 tagacttact agggttagga catctaaata tgatttattt agctttgaaa atagtcgaat
      841 ttgaagcgtg tcgttcaaga gaactactta atattatgtt gattgaagaa ccagaagctc
      901 atattcatca tcatattcaa aaaactctgt tcgaaggatt aaatcttcaa aaaaactata
      961 ctcagattct catgaccaca cattctgtac atttagctga agtgtctgaa atatctcgga
     1021 tgaatgtatt aaaatcgttt aatggcaaat ctatcgttat gaatccagca aataatctga
     1081 actcatttgc taaagaaaag ttaaaaaaga acaatctaaa tctaataaac gcagtagaga
     1141 gatatttaga tgttaaaaga aatggattgc ttttttctaa aggtgttatc ttagttgaag
     1201 gagatgctga agaaatactt attcctcaaa tagtaaaaaa attatttggt ataaacttag
     1261 acgaacttgg aataggtttg attaatgttg gtagtacttc gtttgaatat atcgctagct
     1321 tgtttgacga tgatagaata cagagaaagt gtgctattgt aactgattta gatgaacagg
     1381 caatcccatc agaacatcga ttgtataaaa gcactgcaca agagaaaggt caagcaagaa
     1441 aagaaaaatt atcctccctt tttaatgaaa acaattgggt taattgtttt tatgctaata
     1501 caacatttga aattgagttt ttagatgtga ataaagaaaa tattctaact tatgtcagtc
     1561 caattataga tgacgcattc aaagataaaa aaacaataac tgcttataaa gaagaaatac
     1621 aaagtgaaca atatttagaa agaaatttag cgatgttaaa actagccaat catgttggta
     1681 aggggtggtt tgcaattgag ttaggtgaaa aaattgactt tttaatcaaa attcctaagt
     1741 atattcttaa tgctattgct tttgcatgtc aggaaatagt agatctctca atttataaaa
     1801 aaattctact ccatacaatc gacttgtacg aggaaactac agaattgtca gctttatcag
     1861 ctagtttaaa aacaagtgat acagctgatt tgaatatagc aataatttct ttattagagt
     1921 actttggtaa agatgaggat gttgtgtatc taatcaatca gatagaaaat tacacaaaaa
     1981 tactttatgc tgaggtttca aaatgacaaa tatcttcgaa aaaatcgtaa atgatttaaa
     2041 tttagaacaa aaagatgttg tggaaagtga agataacctt tatgtaaatg catgtcctgg
     2101 gagtgggaaa acaagggtac ttacaaggaa aattgcttac caagcaatta aacacgatgg
     2161 atcactaaaa agaataattg ctatcacata cactaataga gctgctgaag aaattaagga
     2221 acgcctaagt ttacttagta tagatgatga tattaatatt tgggtgggga caatacatca
     2281 gttttgtctt gaatttatta tttatccttt taaaatgaat ctctcaagag tatcaaaagg
     2341 atttacaatt attgatgatt atactcaaag agcttacgtc agccaaatta ataatatgct
     2401 gaatttaaat ctcaaaacgt atgaactgaa caaaattgat ttgtcacttt ctcatgattt
     2461 taaaattaat gaagttcagt atactcaagt ttctgaaatg tatcacacta tgttggaaaa
     2521 aaataaagaa attgattttg atatgatttt actagtagcg tataaaattt taactgtaaa
     2581 cagtaacgtt gcacgtaata ttgctagtaa tataagggcg atttatgtgg atgaatttca
     2641 agatactaga gaattacaat acaatgtaat agctaaatta cttcaaaaca accctcaaat
     2701 tcagtcaatg tttgttggag atattgacca agcaatttat ggatcattag atggtatagc
     2761 taaatcagtt gaagaactag aaatgctaac tagtcacact tttcaatcaa agacgctaca
     2821 tggctgttac aggtctaatc agagattagt agacttctac tctaattttc agagttgtcc
     2881 ttacgagatt gaaagcagag ggaataataa aaacgaccgt ggttttataa gttatgattg
     2941 caaagttact aaggaggatt tacccaacat aataaaaaat ataataaagg aaaagattga
     3001 aagcggcatt catgaaaaag atatttgtgt acttgctcct caacactatc ctctgttttc
     3061 actgggtgag tatttaaaga aagaattacc g
//
LOCUS       DAZUMB010000201         3090 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000201 DAZUMB010000000
VERSION     DAZUMB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.206"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..753
                     /gene="fabI"
                     /locus_tag="ACG80L_002755"
     CDS             <1..753
                     /gene="fabI"
                     /locus_tag="ACG80L_002755"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS5024935.1"
                     /translation="SFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERMK
                     KSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNVS
                     DISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAKA
                     SLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVTI
                     EEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(863..1915)
                     /locus_tag="ACG80L_002756"
     CDS             complement(863..1915)
                     /locus_tag="ACG80L_002756"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS5024936.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2041..2961
                     /locus_tag="ACG80L_002757"
     CDS             2041..2961
                     /locus_tag="ACG80L_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS5024937.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          901 a          631 c          595 g          963 t
ORIGIN      
        1 tcatttctag aaggaaaaaa aatcgtcatt atgggcgtcg ctaacaaaaa aagcattgct
       61 tggggttgtg ctgaagcaat gttgaaacaa ggggcagaaa ttatctatac ttaccaaaat
      121 gaacgaatga aaaaatcatt agtcaaattg gtcggcgaag aagcattcac tgtagaatgt
      181 gatgtttcta gcgacgaaag catcgaaaaa gcaatgaatg ccatcggtga atatgctggg
      241 gagatccacg gccttgtcca tgcagttgct tacgccaaca aagaagaatt gtccggaaat
      301 gtgtctgata tttccagaga cggctatcaa ttagctcaag atatcagcag ctactcttta
      361 attgctgtat caaaatacgc acaaccgttt cttgcaccaa acagcgggat cgttagcatg
      421 acttatctag gttcagaacg cgcaatcccg aattataaca tgatgggtat tgcgaaagct
      481 tctcttgaag ctgctatccg ttatttggca gctgagttct cgcctaaagg aattcgggtc
      541 aatggaatct cagctggggc aatcaaaaca ttagctgtca caggtgttaa agactatcaa
      601 aaactgatcc agttatctga atctcgtacg cctgatcagc aaggtgtgac gatcgaagaa
      661 gtcggtaatg cttgtgcttt cttaatcagc ccactttcca gcggaatcat cggtgatatt
      721 atctacgttg acaaaggtgt ccatctttca taaacttttg ttgcattctt taaagtaaaa
      781 catataaaaa tcaggaaaaa actttttaaa gtctcttcct gattttttct ttttacgttt
      841 ttttcaactc attactttga aattattcca cgacttcaat ccagccttct ggtgcttcta
      901 tgtctccaaa ttgaatacct gtcaattcat catacaaccg ttttgttact ggtcctactt
      961 ccgtttcact ataaaataca tgaagattgt tattgtgata aatcccaccg attggagaaa
     1021 taacagccgc tgtaccacat gcaccagctt ctgcaaattg atctagatca tcaatgaaaa
     1081 tatctccttc aattgcttgc agtcctagtc gttcttcagc taactgcaac aaggaatatt
     1141 tcgtgatact tggtaagata gagggcgatt gcggagtgat gaattttccg tcttttgtaa
     1201 ttccaaagaa atttgctgca cctacttctt cgattttcgt atgtgtggct ggatcaagat
     1261 aaacacaatc tgagtaatcc agttcttttg cttctgttcc tggaagtaaa ctcgctgcgt
     1321 aatttccgcc gacttttgct gcaccagtcc ccttaggagc agcacgatcg taatcagata
     1381 caataaaatt cgttggtttt agtccacctt tgaaatacgc tcctactggc gtacagaaga
     1441 cgacaaataa atattctgga gctggatgga caccaatgtt ttcaccgacc ccaatcagta
     1501 aagggcgcaa gtataatgta gcgcctgttc catagggcgg cacaaaatct tcatttgctt
     1561 ttaccacttg cttgacagca tcaacgaaca tcttttcagg gaccattggc atcagcaatc
     1621 gtctagcact ttgattcatc cgtcgactat tttgttcagg tcgaaagaga ttgaccttgc
     1681 cgtcttttcg acgataagct tttagacctt caaaacattg ctgaccataa tgaagcgctg
     1741 gagagccttc atgaatcgtc aggtaattat cttctgtcaa ttctccttct tcccattgtc
     1801 cgtctttcca tttggcaata aaacgccaag gagttttgat ataagaaaaa cctagattgt
     1861 tccaatcgat ttgcatatac ttaccacctt cgttattttc tccacatttt atcattatcc
     1921 atattatttg tcattttatt ttcagctttt ttccttcatt atagttattt ttgctatttc
     1981 cttttttgaa aacttcttgg tacaatacag aaagaatcaa attttagaaa agaggatttt
     2041 atgttttcat tattagcaac tgccggtacg acagactctt ctggcgagac attgacagat
     2101 caagctgtca gcaaagtaaa tgcgtttcag cggttttggg acgggattga ttgggacgcc
     2161 attttggctg cctttatcca aaaaagttta tatctgattt ttttgatcgt ccttttttgg
     2221 attcttcacc gtgtgggaaa atatctgatt gataagtctt ttaatcagta ttctaaaaaa
     2281 gccattctaa acgagagccg cttaaagact ttacatagtc ttttaaatac tatttttcat
     2341 tatacccttg gtttcttttt tatctatgca attttatctg ctatcggtgt gccgatcgga
     2401 tcacttttgg ctggtgccgg aattgcaggt gtggcaatcg gtcttggtgc ccaagggttc
     2461 atgagtgatg ttattaccgg attttttatt atcatggaac aacagatgga tgttggggac
     2521 tacatcaagc tagcaaactt aacgattgaa ggaaccgtcg tatctgttgg tatccgtacc
     2581 cttcagctga aagcaacgga tggtactgtc catttcatcc cgaatcgaaa catcaccacg
     2641 atcagcaatc tctctcgtgc aaatatgcaa gtacttttag atattcggat tgttcctgaa
     2701 gaaggatatg acaaaatcta tgagatcatt gatcgagtaa atcgtacatt agctgaaaaa
     2761 taccaagaag agctccaaac tgaaccttct atctttggat tagtggatct tggaaacagc
     2821 aattttgcga ttcgtacggt ctgctacgca ttgaacggaa aacaatatgc cttaaaagaa
     2881 gagttcttga gctcctatgt caaagaattg acagcttctg gtttcacgat tccgaacagt
     2941 ccaattgctg tcggaaaata atttggacaa acgatgcaac gaatctcact aaaaagagcg
     3001 tgggacaaaa gtaaagaaca cttttgtccc acgctctaaa cacgtataaa cggcggaagc
     3061 agaagcaact ccttcggaaa taagctgaaa
//
LOCUS       DAZUMB010000202         3030 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000202 DAZUMB010000000
VERSION     DAZUMB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.207"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(250..864)
                     /locus_tag="ACG80L_002758"
     CDS             complement(250..864)
                     /locus_tag="ACG80L_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5024938.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1470..1847)
                     /locus_tag="ACG80L_002759"
     CDS             complement(1470..1847)
                     /locus_tag="ACG80L_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024939.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2147..3028)
                     /gene="galU"
                     /locus_tag="ACG80L_002760"
     CDS             complement(2147..3028)
                     /gene="galU"
                     /locus_tag="ACG80L_002760"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5024940.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          918 a          636 c          467 g         1009 t
ORIGIN      
        1 tccactactt tttatttttc tgacactaca tattcttata actcatttct cataactgtg
       61 aaacaactta taaaaataac aaactttaaa caaaacaagc ttaaaatcaa agatgatcca
      121 tcttaaaata actaaaacaa aaaaagtagt cgttgttaat cctcattaat aatcgttttc
      181 tttttttcaa acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca
      241 tgtggataac tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac
      301 ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt
      361 tggcaaagtc aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa
      421 ttcaaataat cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc
      481 agtaacaatg attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc
      541 cgggtttctt ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg
      601 atcaataatt gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc
      661 aatcaatata cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact
      721 tagtgtgcgg ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca
      781 agtgccagct tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata
      841 atagtcaaaa taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag
      901 aaacagctgc ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt
      961 ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat
     1021 agcaaataaa acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc
     1081 tagattaggt acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc
     1141 agggtgtatg tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag
     1201 ccttttttta ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc
     1261 aagcaagaaa atacaaaaaa acattggtat gatttatata tatataaata acataaaaaa
     1321 aggaatggct tatcccgata caacaagcat tcctttaaaa aagattcttt tatataagtg
     1381 aaaaaagctt ttttccacca attaaataaa tctctcatct caatagttta tccattattt
     1441 tatctttttt tgcgtatacc tactaaatat taccgtcttc ttctcgttct tctactttgt
     1501 tcgagagaat ctgtactaga aaaagtagtt gtatgtctac cttttctgcc tatccgagac
     1561 tttcttttca tatatgggat ataaatgatt agatacagta cagcaaaaaa taaagcaaat
     1621 acgcaagctt cccaactacc taacgccaat tcaaccatca aggtagcaac cccaacggat
     1681 gctgcatacc agataaaaat cagaaacgtt tctttttttc tgaccatgcg aaacatgata
     1741 aatagactaa taagcaatag tggcaacaca aatatccaat tgcttccttc catcagccac
     1801 ttgagtatat caagcagcga ctgtgcaaca gcgactaatt ttcccatagt gaacacacac
     1861 ctttttttat aattaaaaaa ttcgcctttt tattctacta tgaatcttct ggcttggctt
     1921 tcatttctct tatgtaatca ttttctttta tacaacttaa aaaaaaatta aaccattagc
     1981 ataatatttt ctgaatctat tcacgaaagt ctttttctat ctttcgtttt ccacgactca
     2041 actgatctag agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca agctgccgct
     2101 ttaataacaa atagcctttc attctcagca ggccacttat ttccagttaa ctttctgttc
     2161 ttagcttttt actcaactgg ataatatatg cacttaatga agtcccaatt tccgaatgtt
     2221 gaagtccata ttcaatattt gtcttcatat atcctaactt atcgcctacg tcgtatcttt
     2281 ttcctttaaa ctctctcgca tagaccgcct ctgtctggtt aagagtctga agtgcatccg
     2341 ttagctgtat ctcgtttcct gctcctggtt cttgagtttc taatatctca aatattttag
     2401 gagtcagtag ataacgtccg ataatggcta aattacttgg tgcttttccc atttcaggtt
     2461 tttctacaaa cccttttact ctgcaacgac ccttttcgct atctgtttta ggatcaataa
     2521 ttccgtattt agatgtttca ttttctggta cacgcatgac ggcaacagcg gaagcttgtg
     2581 tttcttcata atccatcatc aattgttgag tcaatgggat atcatctttc atcaaatcat
     2641 cccccagcat gacgacaaac ggttcatttc ctataaatgc ttttgcttga agcacggcat
     2701 cccccagtcc tcttggatga gattgacgga tgaagtgaag attgacttga gtcgtttctt
     2761 caactaaagc gagtaattct gttttttcct tttcccgcaa attgttttcc aattcgatat
     2821 ttgcatcaaa gtgatcttca atgggtcttt tttcttttcc cgttacgatc aagatgtctt
     2881 cgattcccga atccaatgct tcttccacaa taaactggat cgttggttta tccacgattg
     2941 gaagcatttc ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg
     3001 caggaatgac tgcttttttg accttcactt
//
LOCUS       DAZUMB010000203         3027 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000203 DAZUMB010000000
VERSION     DAZUMB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.208"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACG80L_002761"
     CDS             202..1077
                     /locus_tag="ACG80L_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS5024941.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ACG80L_002762"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ACG80L_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ACG80L_002763"
     CDS             complement(1642..2973)
                     /locus_tag="ACG80L_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5024942.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         1067 a          549 c          466 g          945 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataata
//
LOCUS       DAZUMB010000204         3012 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000204 DAZUMB010000000
VERSION     DAZUMB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.209"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..359
                     /locus_tag="ACG80L_002764"
     CDS             177..359
                     /locus_tag="ACG80L_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5024943.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="ACG80L_002765"
     CDS             462..746
                     /locus_tag="ACG80L_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024944.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="ACG80L_002766"
     CDS             762..998
                     /locus_tag="ACG80L_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024945.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="ACG80L_002767"
     CDS             1020..1274
                     /locus_tag="ACG80L_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024946.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="ACG80L_002768"
     CDS             1356..1541
                     /locus_tag="ACG80L_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS5024947.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="ACG80L_002769"
     CDS             1608..2003
                     /locus_tag="ACG80L_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS5024948.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            2020..2370
                     /locus_tag="ACG80L_002770"
     CDS             2020..2370
                     /locus_tag="ACG80L_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024949.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="ACG80L_002771"
     CDS             2392..2766
                     /locus_tag="ACG80L_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024950.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DAZUMB010000205         2994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000205 DAZUMB010000000
VERSION     DAZUMB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.210"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACG80L_002772"
     CDS             complement(61..1371)
                     /locus_tag="ACG80L_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS5024951.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACG80L_002773"
     CDS             complement(1383..2801)
                     /locus_tag="ACG80L_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5024952.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          954 a          616 c          457 g          967 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cact
//
LOCUS       DAZUMB010000206         2926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000206 DAZUMB010000000
VERSION     DAZUMB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.211"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..613)
                     /locus_tag="ACG80L_002774"
     CDS             complement(71..613)
                     /locus_tag="ACG80L_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS5024953.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            748..1137
                     /locus_tag="ACG80L_002775"
     CDS             748..1137
                     /locus_tag="ACG80L_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024954.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1141..1905
                     /locus_tag="ACG80L_002776"
     CDS             1141..1905
                     /locus_tag="ACG80L_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5024955.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2034..>2926
                     /locus_tag="ACG80L_002777"
     CDS             2034..>2926
                     /locus_tag="ACG80L_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5024956.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGA"
BASE COUNT          942 a          491 c          603 g          890 t
ORIGIN      
        1 aaataataaa taaaaactgt ctaacattta aaatttaatt tagatattag acagttttta
       61 agtttgtgag ttattttcct tgtcgttttc tcactttttt agtccagaaa ccacctgaaa
      121 tatattttgg ctcataagaa atgataaaag cacgctcttc gatttctttg atcaggcgat
      181 acagttcacg ttcgcttttt ctaggagaaa gtatttcgag aactagccgc tcaccttcca
      241 gccccattgc agaagtctgt gtcacaccat aaccgtgttc tctcagtata cccggcaaac
      301 ttttatcttc tgcgctattt gctggcaaaa tcgttgtgac catgatgtat cctaatgcca
      361 atttgtcttc aatcaaaatt ccgacactga tacctactgc gtaacctaac gcatagacaa
      421 ataaattcag tggattatct aaacgatcta aaacaaggga taagccgacg atataaatcg
      481 tgatctcgac catactgaca agtggagcaa tcagtcgata gcctttcatt gtcaacatga
      541 aacgtaacgt gttcagagtg atataagcaa aattaatgag gaagatcatc cccaacattt
      601 ttggatcaat catttgtaaa actcctttca tttatataac aacaatatca aaaaaaatcg
      661 taagtgaaga aaaattgaaa ctttatacat acaaaaaaat gataaaatga ttataataga
      721 attataaaga taggttaggt gattaaaatg aaaacggtca atcgtgtgtt ttgtgccagc
      781 cacgtgctaa gtctccttct cagtatatac ttaggtgtct attcgattca acagccaatt
      841 gaggtagaga acattgtttt tcagatgtcg ctgacagatg gtttaatttt aagcgttttt
      901 ttctttttga tactgtttat tggtaatatt ttcggtagcg ttgccagttt tttgaatttt
      961 tctatttatc cactcctttc gctagcatta ggagtggtgg gactttgttc tctgtgctta
     1021 tttcccgagc cattcagtcc tgcatttata ttatttggta cactgaacct gttccagaca
     1081 actgttggag catggttgct atggcgatca ggaaatttaa tgaaaatagg tgagtaacaa
     1141 ttgaatccaa ttatattgaa acgttctgtc catcatattc cagtgttaga agttgttctg
     1201 aaagaattaa gagaggaacg tattcccgtt gtcatctatt atcacggatg gcagacaagt
     1261 aaggagttag ttttgacgca aggaagaaaa attgcgcagc agggcattcg tgtgatcctg
     1321 ccagatgcga tgaatcatgg ggagcgcaaa cagccggttt cttctattcc atcctataca
     1381 ttttgggata gtatctacgg aaatctgttt gaatttgaca cacttatcaa gcatttagaa
     1441 aaaagaggtc ttctacatga tcagcttgca gttggcgggg tttcgatggg gggaatgaca
     1501 acatgcgcct tgttggctaa acatcctcat atagtagcgg gcgtgtgttt gatgggttcc
     1561 cctgcacctt tggaatatgg aaaagaaatc gtaaggcgcg catcggaata taattatgtc
     1621 gtaccaaaag actattttga tttgatttca tgggttaaga gctatgattt gtcattgcag
     1681 ccggaaaagt tagctggacg tcctctgttc ttttggcatg gacaagctga cgaaaaaatc
     1741 ccttatatac aaacggaacg atttgtagaa aaaaatcggc ataagtctta cggaaaagaa
     1801 attgtttttc gttcctcaaa aacacagcga catatggtac aaataccact aatggaagaa
     1861 gcttccactt ttttagcaaa aaatttgata aaaaagtata aataattgaa acggaaaatc
     1921 actcatgacg atagaggatt ttccgttttt tattttgggc tataataaac tctgttagga
     1981 aaatttggta taattgatag taagaatgac aacatgttag aaaggaagag agcatgccca
     2041 ctttagcaga tgttgcaaaa cgtgccaatg tttcaaaaat gacggtctct cgagtcatca
     2101 atcatccgga acaggtgact gacgaattga aagagttagt tttctcagca atggctgaac
     2161 tggattaccg tccaaatatt gctgcaaaag cacttgtcac gaatcggtcg cagatcatca
     2221 aattatttat actggaagaa atcgacacaa ctgagcccta ttacatgaat ttgctgatgg
     2281 gaatcgctaa acggatcggt aaagcgcatt attctctcca attagttaca aatgatgcct
     2341 tcgacgtcgg ttcttgtgat ggatacatca tcacaggtgt cagaaaaaaa gacttcgatt
     2401 ggatcgaccg tttggaaaaa ccagttgtct tgtttgggga aaattctgaa ggatatgatt
     2461 atgtagacag cgataatgaa tatggtacag caaaagcaac gcaatatgcc ttagaaagag
     2521 attatcaaac gattatttat atcggtatcg aagtggacga gccttttgaa atttcaaggg
     2581 aaactggtta tttatctgtg atgaaggaag ttgatcttcc tagtaaaatc ttgcggtttc
     2641 caaatcattc gacagaagta gaaagatata tgacagaaaa ctgggcagat tttacagata
     2701 aaacttgttt tgtatgtagt tcagatcggc tagcactagg agcagtaaga gggattataa
     2761 atgctggagg acacttgccc gaagaatttg gtgtgattgg atttgatggg gtatttttag
     2821 accaaatcag ttcacctaaa ttgacaacga tcaagcaaga tattatacgt atgggtgaag
     2881 tatgtggaga aatgttgtta aaaaaaatag aagaaaaagg ggcgag
//
LOCUS       DAZUMB010000207         2886 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000207 DAZUMB010000000
VERSION     DAZUMB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.212"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            798..1052
                     /locus_tag="ACG80L_002778"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ACG80L_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ACG80L_002779"
     CDS             1122..1634
                     /locus_tag="ACG80L_002779"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5024957.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ACG80L_002780"
     CDS             1631..2668
                     /locus_tag="ACG80L_002780"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5024958.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1041 a          513 c          574 g          758 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctac
//
LOCUS       DAZUMB010000208         2851 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000208 DAZUMB010000000
VERSION     DAZUMB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.213"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(880..2220)
                     /gene="glnA"
                     /locus_tag="ACG80L_002781"
     CDS             complement(880..2220)
                     /gene="glnA"
                     /locus_tag="ACG80L_002781"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS5024959.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2248..2640)
                     /locus_tag="ACG80L_002782"
     CDS             complement(2248..2640)
                     /locus_tag="ACG80L_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5024960.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          861 a          609 c          460 g          921 t
ORIGIN      
        1 gaaaatcgtt actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta
       61 tgatagataa tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat
      121 atagcttatc gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa
      181 acgtagtcca cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca
      241 aaacaattcc actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg
      301 attcattgaa ggtaacgaat cacataagta aaaaataagg agatgcgaca aaagtcgtgc
      361 ctccttattt ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
      421 ccatcaaaca tgagaagctg tttttgaaat tatttcttgt gattcggagt taaatggtag
      481 gctcacaacc tctggataaa cggtattcaa aagccagttt ctcggtatta agccaaattt
      541 atgcaaaaat gtagaaagcc attttcacaa aatttttctt aatactcgaa actaaacgtc
      601 tttttcaaaa cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagttcag
      661 ccaattcaaa aacaagaaaa gctgtttttg cttggctatt cttattactt gttgcagggc
      721 agctttttca caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc
      781 aaaaaacggc aaaatatgag gaactatttt tccgttagtt tctctaatac tcaggtcaaa
      841 acgctgattt cacagcctct aattcacggt attcctgttt taataaagtt ctagatattg
      901 ttctctttcc cactcggaaa cagtttgtct gaatgccgcc cattcgagac gttttgcttc
      961 aacaaaattt gagaagatat gttgtcctaa tgcgtcaatc attacattat ccttacgcaa
     1021 ttctttgata gcattatgga tagttgaagg taagtcctgg atatgtgcag cttctctttc
     1081 ttcctcatcc attacataaa tgttacggtc aacagctgct ggcggtgtga tctgattacg
     1141 gataccgtct agtcctgctt gcaacaatac tgccattgcc aagtatgggt ttgcagatgg
     1201 atcaactgac cgcaattcca aacgtgtgga taaaccgcga gattctggta cacgtactaa
     1261 tggcgaacga ttacggccac tccatgcgac ataaacaggc gcttcataac ctggaacgag
     1321 acgtttataa gaattgactg ttggattgca aacggctgtg taggcacgag catgtttcag
     1381 caaaccacct aagaaatgat aagctgtttc acttagttcc aattcaccgt ctttatcata
     1441 gaaaacattt ccttcatcat tgaacaaaga catattgcaa tgcataccag aaccattgat
     1501 cccaaataac ggtttcggca tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt
     1561 gacaacgagc ttgaaggttt ggatattatc acaagcatct accacatctg catatttaaa
     1621 gtcaatttca tgctgtcctg gcgctacttc atgatgtgaa gcttccactt caaagcctag
     1681 actttctaat tccaacacga tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa
     1741 atcaaaataa ccacctttat cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt
     1801 aaataagaag aattctggtt caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat
     1861 atctttcaat gctcgtttca aattcccgcg aggatcgccg gcaaatggtg tcccatccgg
     1921 attatagata tcacaaatta aacgagcaac ttttccatga gcactttccc acgggaagat
     1981 catccaagtg gagagatctg gatacaaata catatcgctt tcttcaattc gaacaaatcc
     2041 ttcaatcgaa gaaccgtcaa acatcatttt attttctaat actttgtcga tctggctcac
     2101 tggaacttct acatttttga tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg
     2161 tacgttttct tcctcaatga ttcgtttgat ttcttcacct gttatctgtt tctttaccat
     2221 ggtatccctc ttcctttttt aatttattca gtcaaagaat tgtctttgca atccttgcga
     2281 ctgataatga cctgttttgc ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg
     2341 tcgaacgtca tgatctgtca acggcttctt gttttttgct tcttgttttt tcgcctgtgc
     2401 catttcgtat acacgtttga tcccagccat attcaatccc tctgaaagat agtctttaat
     2461 ctcaagcaac atatcaatat cattcagcga atacatacgt cggtttcctt cgcttcgttc
     2521 cggatgaact aaatcttgtt cttcataata gcgaatttgc cgagcagaca aatcagtcag
     2581 cttcataact gtaccaattg gaaatacgga catagatcgg cgcaattcct tttctcccat
     2641 cagacgtccc ctttcgtttt ataagttggc taaagtgtac caattaaaaa aaatattgtc
     2701 aactgcatat gttaggtttt ctaacatgag atctctattt tttataaaat aacaaaaaaa
     2761 taagaacaca aaaaaggtat ctagttgatt ttcgtaccag atacctttta ttttttatta
     2821 tactgtacta tcctttatta gtgtttttat t
//
LOCUS       DAZUMB010000209         2846 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000209 DAZUMB010000000
VERSION     DAZUMB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.214"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1170)
                     /locus_tag="ACG80L_002783"
     CDS             complement(76..1170)
                     /locus_tag="ACG80L_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS5024961.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="ACG80L_002784"
     CDS             1359..2120
                     /locus_tag="ACG80L_002784"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5024962.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="ACG80L_002785"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="ACG80L_002785"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5024963.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          910 a          560 c          474 g          902 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctct
//
LOCUS       DAZUMB010000210         2824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000210 DAZUMB010000000
VERSION     DAZUMB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.215"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACG80L_002786"
     CDS             102..2543
                     /locus_tag="ACG80L_002786"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS5024964.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          939 a          487 c          657 g          741 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gact
//
LOCUS       DAZUMB010000211         2810 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000211 DAZUMB010000000
VERSION     DAZUMB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.216"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(275..478)
                     /locus_tag="ACG80L_002787"
     CDS             complement(275..478)
                     /locus_tag="ACG80L_002787"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024965.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(460..993)
                     /locus_tag="ACG80L_002788"
     CDS             complement(460..993)
                     /locus_tag="ACG80L_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5024966.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1014..1958)
                     /locus_tag="ACG80L_002789"
     CDS             complement(1014..1958)
                     /locus_tag="ACG80L_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5024967.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            2595..2681
                     /locus_tag="ACG80L_002790"
     CDS             2595..2681
                     /locus_tag="ACG80L_002790"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5024968.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
BASE COUNT          760 a          501 c          483 g         1066 t
ORIGIN      
        1 tatactacca agtaacgcct gtaccaaggg atccaggcgc tgattttgtg aatttttatt
       61 ggtctaggtt aaaagctctt tttaaggctt ggttttctgc ggttgtttct ttctaattct
      121 ttgtgaatat tgatcggtca ttttttaaat acttgttatt tttgtttgtg tttgttatgc
      181 tttttacata tctcatattc atttattatt cttttctaag gttaataata ggataatctc
      241 tttcagaaag actgctcgta cgagcagtct tttcttattt atcaagtttt tctacaaata
      301 cttcctcatt ttgactgttg tagtaattag gattgagttt tagttcccct tttttatttc
      361 ctttgttggc cactgataat aacattgggc taaagcttaa tattcctagt aggatagaac
      421 cgaatagcaa gtgttttttt gtcataagta tttccttctt catttccata gctgccacca
      481 ttttttccaa ttctttgttt gttcgtccat tgtttctgtt gcttcagaaa gagtcgttga
      541 actattctct gaagactctt ccgtataagt caactgcagt tgctcctgaa gtttttcgat
      601 ttgttggttg gcttgcaggg tgagttgttg ttgctggtcg agtagttttt gtatatgaga
      661 attattttgc tctttgaggg caagttgttc tcgtaaaaat tcattttctt cacgcaactg
      721 atctagcaac ttgtttgcaa ccacgttttt gtttggtata taagcatttt tatcgttgtt
      781 ttgttcatgt ttatgcaact tatctagcaa cctttgattg tctaagttgc gagcaagttg
      841 caatactcca gaacgcaata caaccttata accacgctca tttttagcta tcatggaaga
      901 atctaatttt tggatatgtt tcgatattgc ttgcctactc atcttaaaaa gatctgacaa
      961 ttcctttatt gtaaaggttt cttgtggttg catactggtt gcctcctagg ttgctactgg
     1021 aataaattat ctaaatcaga taataaactt tttttctctt tttccgaaag tgcggactct
     1081 gtttgttttt caagagcttt tttctctttt tctagttgtt tgatttgttg gaggagttca
     1141 agttctcgtt tcttctgttt gattttttcg ttaattgttg tagcgggttc ttcgtattct
     1201 ttttgtttat cagctaaagc caatgcttta ttttggtctt ctttggttgg tgcatgtttg
     1261 ttaaagtcgt ctgctttttt gatctcaggt ttgaacgtaa attgatagcc aataacaggt
     1321 tttccccgtc cttttccgta tttctttttg attgttagtc ctttgaagat tgcagttagt
     1381 tcttgacgta taggttttat aactcgttta tctatatcag gttgcttata acttttagga
     1441 atatctaata attcaagaaa gtcttctttg gagaaatatg cgaaaccttt ggttctgtat
     1501 tgctttaaca gacgaaacat agtctttgca tagccacttt gtagctcgtt gaattgttgt
     1561 aaagaatagc gtacccactc gtctaaatta tttaacaaag gcagtgcttt ttcgtaaatt
     1621 cgtatatcta cgtaaggatt ttctacgtcc ccgttgattt taaattctgt aaacattaca
     1681 aacatatcgt aagaaagccc agactcactt ctacgaccaa agcgtaaaga taagattttt
     1741 tcataagttg cttttaaatc ttctacaaat cgtacatttg cagttgcttt gtagttactt
     1801 aaatctttta gttggtcaaa cgtaaaacga actgtcttat cccctttttc acgcattcta
     1861 gacacgatgg aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta
     1921 tttaattcat ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat
     1981 gaatgacatt ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc
     2041 ttcataaaca gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa
     2101 atgtccttca taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag
     2161 gtttaaaaag aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa
     2221 accacttctt ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct
     2281 tgcttcctac ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc
     2341 gcctaaaggc tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg
     2401 cttatctaag ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc
     2461 ttacaggcgg tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac
     2521 acaagtttaa ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga
     2581 aggaggtgta tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct
     2641 ctttattcag ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc
     2701 aacctgaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt
     2761 gatcatttgc atgtttagtt taacatgatt gccgatcaaa ttcaattttg
//
LOCUS       DAZUMB010000212         2794 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000212 DAZUMB010000000
VERSION     DAZUMB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.217"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..491
                     /locus_tag="ACG80L_002791"
     CDS             66..491
                     /locus_tag="ACG80L_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024969.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(979..1680)
                     /locus_tag="ACG80L_002792"
     CDS             complement(979..1680)
                     /locus_tag="ACG80L_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5024970.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1696..2136)
                     /locus_tag="ACG80L_002793"
     CDS             complement(1696..2136)
                     /locus_tag="ACG80L_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5024971.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(2136..2711)
                     /locus_tag="ACG80L_002794"
     CDS             complement(2136..2711)
                     /locus_tag="ACG80L_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS5024972.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          865 a          559 c          447 g          923 t
ORIGIN      
        1 catccgtttg aagttttaca ttccaatggt ataatttctt tatcctatca aatgggggaa
       61 gtattttgaa gaaattaatc gtactattta ttttgtctgc gggacttttg acattgactg
      121 gctgtgattt gtttggcggc tctgaccaat cctcggacga aacaaccgat tcttctgtct
      181 taaaagcgga aaaagcagat accaccaaca gtgtatatac gctaaaagta cgtaaagata
      241 tggatttatc cgcgggacag ttgacattta cagataaaaa aatggaatgg acacgcacgt
      301 atcagacaca aggaactgat tcttctgaat catctgaaga aaaaacagtg ctgacagata
      361 tcaagatcac taccaaagat gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa
      421 caacagtcat atttacaaaa atcggtaatt accgtttgaa ggatgaggat ggcaacatct
      481 acagtctata gtgccgtctg ttggatcttc ttttgtttct ccccttcttt tctgctatag
      541 taaagaagca agatgattgt aaaggagacg aaatggatga tcgatagtcc tgattattgt
      601 acatgtaata acaacaaaaa tatccataca gaatttaatg accaaggaag cttgagcata
      661 tgcgacaact gtcacaaact agtcaaagat tcatttaaaa catttgaatc ttatcctgtt
      721 caaaattatg taaaaagtag aagaagataa acaaatccaa gcaaaactca ctatggctga
      781 tttttatgaa cttcaaactc agcttcttcc tttgatcaag cttcatcagt aaaacaaaca
      841 gtggatcatt tcagtttaca aatcaaagag taaagattta tttatttcaa atatttgaat
      901 ataaaataac caaacgatag aaaggcttat tctacatcac gtagtaattt ttctttcccg
      961 tcgtttggtt ttttattttt agcctaattc ttgacctttt gcatgctggt tatccgtaaa
     1021 atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct gacggatcac
     1081 cttttttgca tcttctggat tttctacaaa atcgaattta tccccatcca cttgtatttt
     1141 aggacataca tcaaaatctt cataccattg attatagcgg gtgttcaacg tttcataata
     1201 ggtatataac gtctcatcgc tttctagctg ttcatagtca cgcccacgtt ttttgatacg
     1261 ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggcc gtttttttgg
     1321 agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact gctgtacttc
     1381 gatatccgtt acccgaccta aatcagcgtt tagatggaac aacaggctat cttcatagat
     1441 ggatcggtct agcacattgt catcgttcac taaagcattt ttcattgcta aaaatcgctt
     1501 gttcaaaaag aagatttgca ataaaaaagc gtactgctca ggatttgaat aaaagagcgg
     1561 caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct cttttgcaag
     1621 catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa tcactgacat
     1681 acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca atgtttcaaa
     1741 atcatattca cgtacagctt ggtctgattt taaatacgta tggagacttt gtgtaatcat
     1801 caagttcgta ggtaccgtct cttcttgtac aatgatcatc ggttttttca acatagttgc
     1861 cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt caaggatagc
     1921 aacaacaaat tctgcatttg tcagttcttc catatctttt tcgtatactt cctgcgccca
     1981 tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt agaaacttgt
     2041 gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga tttgctcctc
     2101 tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt caataataac
     2161 tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt gttggaaaag
     2221 attacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt ttctaataat
     2281 tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt gtcgggcgtc
     2341 agaccgattg ctatccaaga aagaagttgt gagaattcct ccaatgtacc tagacctcca
     2401 gggaaaacga ggacagcatc tcccttttca aataataata cctttctttc atcgatcgtt
     2461 tctgtaggaa taaatgtagt aacatctttt ccaggcaatt cagtttcaaa aagcccattc
     2521 ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat cactcccata
     2581 atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat gtattttcct
     2641 aacgtttgcg caataaattt gtaactttct ttattaccaa aacgggaacc gcaaaaaaca
     2701 gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct acacgtgttt
     2761 ttttaaaata aaagactaga ttttgtagtt tttt
//
LOCUS       DAZUMB010000213         2777 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000213 DAZUMB010000000
VERSION     DAZUMB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.218"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..351)
                     /locus_tag="ACG80L_002795"
     CDS             complement(13..351)
                     /locus_tag="ACG80L_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS5024973.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(364..549)
                     /locus_tag="ACG80L_002796"
     CDS             complement(364..549)
                     /locus_tag="ACG80L_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5024974.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQEHIETVKKILGIQ"
     gene            852..1226
                     /locus_tag="ACG80L_002797"
     CDS             852..1226
                     /locus_tag="ACG80L_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5024975.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            1231..1659
                     /locus_tag="ACG80L_002798"
     CDS             1231..1659
                     /locus_tag="ACG80L_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5024976.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            1709..2362
                     /locus_tag="ACG80L_002799"
     CDS             1709..2362
                     /locus_tag="ACG80L_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024977.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETNISKTKET
                     TEISSSTSKSTSKTDSSSTVTSSNQATAKPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNTEAQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            2499..>2777
                     /locus_tag="ACG80L_002800"
     CDS             2499..>2777
                     /locus_tag="ACG80L_002800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024978.1"
                     /translation="MKKNLILGFICTSILTLGACSSPSNNATNSETQKTINSLKKENE
                     ELKKQIASQSNDTEENSESIDNASKEKTLPVVGILEYTISSIKTEQVTN"
BASE COUNT         1004 a          485 c          456 g          832 t
ORIGIN      
        1 actagcgctc tattaaattt tcccatccgc ttgcctcctt aagatttccg aaaaatttgt
       61 ttctaaaaat tcaagtgttt tacttcttaa aaatagatat gtgtctcttc cttcaactgg
      121 ataatagaca aatccatttt tgtttttctc gatatcgata atatttctat atcttgggtt
      181 ctttaaaact ctagaagtaa accaatcata ttttctgtta atccgttcta gcacttctgg
      241 caacgtcatc catctaccag tatcatcagc tttttttaac tcctcgtaat ccacttgaga
      301 gataattaca tagccttctg gaactgggat tttcgcttct agatattgca ttagctgttc
      361 ctcctattga atacctaata ttttttttac agtttctatg tgttcttgag cttttttacc
      421 atcacgattg ccgtttagga tatctgataa ataagctcct gaaataccaa caagcgcagc
      481 tagttctttg aaagtcattc ttctttttct catctccgct cgaattttta agtctaaatt
      541 ctcagacata aaaatagctc ctttctaaaa aataatttgt aagctaaaaa attagctaaa
      601 tcgttgacac ctattagctt ttaagctatt atgaatacat agttaaataa gacttataaa
      661 aagcctctaa aataacattt ctaagtttgg cgaccgagag aatgttttaa attagtagag
      721 ttttttgttg ctcttattta gctaacaatt tagcttacga attaaatata ctagcttaaa
      781 agctaattgt caaccaaaaa tataactttt aagctattta ttttcttttc agctttgaaa
      841 ggttgataat aatgagttta gttactaaga ttaaagaatt agcagacgaa aagcatgtga
      901 ctatagcaga agtagaaaga caggtgggca tctctaatgg acaaataaga agatgggata
      961 aagcctcgcc aaaatctgag aacttaaaga aagttgctga ttattttggt gtcacaactg
     1021 attatttatt gggaaataat aatgttccca aatgggctac aaaagaggaa gtggttgaac
     1081 ttgataaact actagactca aatgttaata tgtcttatgg tggggaaaca ttgacacccg
     1141 aacaaataca gcgcgtaaaa gatatcctga tagcgacttt ctgggatatt gtgaaagaag
     1201 acaaagaaaa aggcaaaaag atgtgagctt atggagatgg atacgattaa tttagtcgag
     1261 gagttgaagc ggaaatacca gtccgctaat cctttttata tttgtgaaaa gatgggcatt
     1321 aaaattcaat acgttccttt tatcgagaat cccaaggggc agtttcaaga aattagagat
     1381 cgtgcgataa tctttttaaa tgatgaactg cgagactctg aggaaagatt ctacatttgc
     1441 gctcacgaat taggtcacgc tatttttcat cgtggcttat ccagctatta tgtatcaaca
     1501 agaacatcta gaagcaaatc tgaaagcgaa gctaattgct ttgctgctaa tctcattgtt
     1561 tctctttata aagaagataa tgatcaatat cctaaaaaaa tcgaggaatt aaagaatctt
     1621 tacggtcttc cagaaagcgc ttatcgtttt cttatataaa aaagcccgtg ttagcacaca
     1681 tattacaacg agaaagagga attataaaat gaaaaaagtt agcgttatgt tgttgttaag
     1741 tactgctctg ctactttcag cttgttcaaa taataaaaaa gctgaatcaa cagatgccac
     1801 ttctaaccaa gaaacaaata taagtaaaac aaaagaaaca actgaaatca gttcatctac
     1861 tagcaaatct acatctaaaa cagattctag ttcaacagtt acaagctcca accaagctac
     1921 ggcgaaacct agcccaacag ttataagctc cagtcaggat acaatccaaa ccgcacctca
     1981 agaagaaaca tatgaacaga tgaaacaacg cactttacag tcaactccag ctgatcgtgc
     2041 aaattggtcc aacaaagagt gggaagcttt cggcgtggcc ctttatgaaa atggattgac
     2101 tacagatgat gctggcaata ttatcagtca agatcagaaa gaacaacaag ctgcatctca
     2161 acaaaataca gaagcccaac aaacaagcgc tcaacaagac gctgacactt tatcacttac
     2221 tgattttgtt aacaaatacg ggatgtcgcc tgttgcatgg aaagtacaga atggaatgtc
     2281 tgaagaagaa gcattgcgta caacacagca aaaaacttcc ggtgaagttc aattaggatt
     2341 ttctaaatac ggaatacaat aatatatttt tgtgtcccac tatcttgcct ataacctcta
     2401 aaaagttata aagaaaaaag cccgtgctgc aacacggact aaaatctcat ttctaagatt
     2461 ctacataaaa ataatatcat agaagtgagg aattaaaaat gaaaaaaaac ttaatattag
     2521 gatttatttg tactagtatt ttaactttgg gtgcatgtag ttcgccttca aataatgcaa
     2581 ctaatagtga aactcaaaaa acaatcaata gtttaaaaaa agaaaatgaa gagcttaaaa
     2641 aacaaatagc atcgcagtca aatgacactg aagaaaatag cgaatctatc gacaatgctt
     2701 ctaaggaaaa aaccttgcca gttgttggta tcctagaata tacaatttct tctataaaaa
     2761 ctgaacaggt aacaaat
//
LOCUS       DAZUMB010000214         2740 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000214 DAZUMB010000000
VERSION     DAZUMB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.219"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1634
                     /locus_tag="ACG80L_002801"
     CDS             <1..1634
                     /locus_tag="ACG80L_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024979.1"
                     /translation="YSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNY
                     GDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDA
                     AFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSI
                     SLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFW
                     YPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFI
                     LKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIIT
                     LVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTT
                     SDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTV
                     PVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILT
                     YCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1621..>2740
                     /locus_tag="ACG80L_002802"
     CDS             1621..>2740
                     /locus_tag="ACG80L_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS5024980.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYL"
BASE COUNT          913 a          407 c          455 g          965 t
ORIGIN      
        1 tatattcagt aaattttatg agaaatgctc aagaaattta tacaacaggt gatttatctt
       61 ttcatctttc acgaatcaaa gggttatctt ctatttttga agggccaatc aactatacaa
      121 catttaacaa ttatggagat ggactgaact atttttatcc ttttcttaca ataattcctg
      181 cagtcgtttt ttatggaatt tcaaataact taatattaag ttatgttcta tatatttggt
      241 tattaaatat ttgtacgatt ctgattagtt tttattatgg agaaaagttt tttaaaaaga
      301 tagatgctgc ttttttattt agttgtttat atacttttgc aacttatcga acagttgata
      361 tctattatcg atccgcaatt gcagaagcaa tcgctcttac gattatccca gctgttttat
      421 attatgctta tggattgata ttcaatagag aaaaaaaagt catcgcttta tctgtcagta
      481 taagtctact tgtgtatact catgtcttaa ctacactatt gtgtagtgca ttgattgctt
      541 gtatgattat agttcgtttg tttttaagca agcaaataaa aaacgatata aaatttgtgg
      601 tcgtgaattt agtaaaggct gtttttttga cttgtgcatt aactagctat ttttggtatc
      661 ctatgttgca gcagatgaat tatcaagaaa tacacagtcc cttcaaaaca gatttgcagc
      721 tagaagcact ttctatttca gatagtatca ttcaagcgtt aaataacgat attacaacat
      781 acactatggg gttattagga ttggttgcgt tgatttttcc tttattcata ctgaaagaaa
      841 tggagaaaaa agaaaaaatc atctatgttg gcgtagtttc tacatgggta tgcgtaacta
      901 atttatttcc atggtttttg tttcaaaaaa caccgataag cttactccag tttccttggc
      961 gtatcttagg tatccaaatc tttttttctt caatgatcat aacgcttgtt tttatgaaaa
     1021 agactatgaa taaaaaatat tcatggttaa tcattggcgc tactgtggtg tttctactcg
     1081 tcacgtctat agcagcaaaa gaaaattacg caaaagttat ccaatcaaaa cacggtcata
     1141 ttgttatcaa tgaagaaaca ctcccacgtt atacaacatc agatatggga ggattatacg
     1201 attacgcccc aacagaagcg ataaaagaaa ggcctcactt agaaaaacat gaagtaaaag
     1261 taggagaatc gtgggaaaaa gggaagtata aagctgcaga taataacatt acttacacag
     1321 tggcaagcaa caaaaagcaa aaagtgacag taccagtata tgcttattta ggtacacagg
     1381 taaaagtaaa cggtaaagtc gtaaatgact cttataaaaa acatggctta gtgaatgtgg
     1441 atgtaaatgc tggagaaaat aagatagaaa ttacaacaaa atatactcca actgctctca
     1501 tggctttatg gatcagtatt ctgacttatt gtttagtggt gtattcaagt tttaaacgat
     1561 ttgcacctaa taagttgaaa gattctcgat atgtgactaa aaaggagata aacaagaaga
     1621 atgaaaataa ataaaaaagt agtaatattg agtggattaa ttatagttag tagtatatat
     1681 tctttcatct ttttcaaaaa tacacagagt atttatacca ctggagatct ttcttttcac
     1741 ttatctcgaa tcaaggggtt atcgactatt tttaccagtc cgattaatta tgagacattc
     1801 aattatactg gatatggtgt aaattatttt tatccctttc tgactttttt tccagctgta
     1861 atgttatatt ggatgacaaa aaatttgatt gtaagctata ttatttacgt ttggttattg
     1921 aacttgtgta caacactcgt agcttatcat tatggcgagc ggtttttaaa acaaaaaaaa
     1981 gcggcatttt tatttagttg tctctatatt ttctctgctt atcggacagt agacatctac
     2041 tatcgttccg caattgccga agctatcgca ataacacttg ttatccctgt attattttat
     2101 gcttatcaaa tcatctctgg aaaagaagaa aagtatccgt ctgtaaagct agcattgagt
     2161 atgagtttgc tagtgtacag ccatgtacta tcaacattaa tgagtacagc attaattatc
     2221 atttttattt ttattcgttt ggtttctaaa ggtttcaaga atgcagattt tattgctata
     2281 tttaaaaaat tatttagtgc ggcaggaatg acattggttc tgacgagtgc tttttggtat
     2341 ccgatgtttg aacaaatgct ctaccaaaaa atcaacaaac ctagcgtgac aaatttatat
     2401 gctcatgcat ctaatgtgtt cgatagtctg actgaagcta tgaacaacga cttaactact
     2461 tactcaatgg gactagttgg tctgttatct ttatgtattc ccctgatttt atttaaaaaa
     2521 ttaacaaaca tagaaaaaaa gatttattat ggtacgtgtt tgacttggct agctacaact
     2581 agtttggtgc catggtatct tctgcaaaat acgccagcca aattgttaca atttccttgg
     2641 cggatcttaa gcttgcaaat aatattctct tctctgatat taacgatgat atttttcaaa
     2701 aatagaagat acaataagac aagagaacta ttttatttat
//
LOCUS       DAZUMB010000215         2729 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000215 DAZUMB010000000
VERSION     DAZUMB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.220"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..519
                     /locus_tag="ACG80L_002803"
     CDS             175..519
                     /locus_tag="ACG80L_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS5024981.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ACG80L_002804"
     CDS             520..939
                     /locus_tag="ACG80L_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS5024982.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ACG80L_002805"
     CDS             complement(1031..1381)
                     /locus_tag="ACG80L_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024983.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2677)
                     /locus_tag="ACG80L_002806"
                     /pseudo
     CDS             complement(1347..2677)
                     /locus_tag="ACG80L_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
BASE COUNT         1012 a          491 c          527 g          699 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttg
//
LOCUS       DAZUMB010000216         2525 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000216 DAZUMB010000000
VERSION     DAZUMB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.221"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1394
                     /locus_tag="ACG80L_002807"
     CDS             <1..1394
                     /locus_tag="ACG80L_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5024984.1"
                     /translation="KVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYE
                     GVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPAL
                     PEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPE
                     GQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSD
                     EVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVD
                     VSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNA
                     EVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNK
                     KDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTL
                     GLLGYKNRKKENE"
     gene            1528..>2525
                     /locus_tag="ACG80L_002808"
     CDS             1528..>2525
                     /locus_tag="ACG80L_002808"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5024985.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSI"
BASE COUNT          991 a          352 c          508 g          674 t
ORIGIN      
        1 aaaaagtaga agttgggggc acagtagatc taacagataa tgttactaac ttgccagcgt
       61 taccagaagg aacaacagta acagatgtta ctcccggcgg tacaattgat accaacacac
      121 caggtaatta cgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa gatacggtga
      181 aagttccagt agaagtgaca gacaatcgtt ctgacgccga taaatatgaa ccaacagtag
      241 aaggcgaaaa agtagaaatt ggaggcacag tagatttaac agataatgtt actaacttgc
      301 cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca attgatacca
      361 acacaccagg caattatgaa ggtgtcattg aagtaacgta tccagacggt acgaaagata
      421 cggtgaaagt tccagtagaa gtgacagaca atcgttctaa tgcagataaa tacaatcctg
      481 aaggacaaaa agtgactaca gacttaaata aagagccaga tgcatctgag ggaattaaga
      541 acaaagaaga tttaccaaaa ggaactactt atgcttggaa agagaaagta gatgttagca
      601 cagctggaaa taaaaaaggt actattgtag tgacatatcc agatggatca tctgatgaag
      661 ttgaagtaga cgttacagtg acagatactc gttccgatgc agataaatat aatcctgaag
      721 gacaaaaagt gactacagac ttaaataaag agccagatgc atctgaggga attaagaaca
      781 aagaagatct accaaaagga actacttata cttggaaaga aaaagtagat gttagtacag
      841 ctggaaacaa aaaaggtact gtagtagtaa catatccaga tggatctaaa gaagaagtag
      901 aagttactat ttctgtagta gataaaaaag caccgaataa acctcaagtt gatcctatta
      961 cagagggtga ccaaattgtt actggtaaaa ctgaaccaaa tgcagaggtg acagttacat
     1021 taccagacgg aagtcaacac catgggacag ctgataaaaa tggtaatttt acagttaaag
     1081 ttcctaaatt agaggcaggg acaaaagtga tagtaactgc aactgatgaa tctggtaata
     1141 ctagtgaacc tactaatgta gttgtttctt ccaacaaaaa agacagtgga aaaaacggta
     1201 gtaaaggtag taagactgac aatcaaggta gcaacagcaa tcaagacaaa aatagaggca
     1261 aaagccagtc ctcaaaagtt tttcctaaga cgggagagag tgacagtaat atctttacta
     1321 ttagcggtgg tttaatactg ttaggaacgt taggattatt agggtataaa aatcgaaaaa
     1381 aagagaatga atagaaaatc aatttcttaa gtcttctctg agaagaggaa gaatcttttt
     1441 cttcctcttt ttgctttttt atgatgggaa ataagtgtta gttaaagatg aatatacttt
     1501 ttatagaaag gaaaagaatg taattaagtg gaaaaaatag taattattgg ttcaaaccat
     1561 tcagggctag ccgcagctcg atttctaaac ttaagcgatt caaattatga agttattcta
     1621 atcgataaaa aatcagaaat tagttacttg gaatgtggga catcgttatt tataaaagga
     1681 gaagtaagta atttagaaga tcttttttat caaaatagaa tgacactaaa ttctgagata
     1741 aaaaagttat ttctagagac agaagttgac aaaattgata ttcaaaataa aaagattagt
     1801 tgtgtaaaaa aagatggtac aagattctct caagaatatg acaaagttat attagctaca
     1861 ggttcaaagc aaattatgct agatataccg gggaataatt tagctaatat tttttcgata
     1921 aagaattttc caaatgcaaa agagattttt aaagctttgg atgatagtac tattaaaaaa
     1981 gtaggaatta ttggagcagg atatattgga gttgaaattg ctgaagccat aagaaaaaga
     2041 gggaaagaag tatatctttt cgatgttgca gatagagtgt tgtcgactta ttacgaccgt
     2101 agtttttcag ataaagttga agaaatacta tcaaaaaatg gaatacatct ttgtctatca
     2161 gaaagtccta aaaaatattt aggaagaaaa aagattgaaa aaatagtcac tacaagtggt
     2221 acccaatact ctatggatat ggtcgtacaa gctataggat ttctatctaa tagtccaatt
     2281 ggggaaaagg aactcttgag agatgttagt ggagcatatc taactaatga ataccagcaa
     2341 acaaatataa aagatatata tgctattggg gattgtgcta caactttttt tacgtccatt
     2401 aatagaagta gtgttgattt ttctatttct aatgctctaa gaacagctta tattgcaact
     2461 caacatatta acaatcaaga ttttacacct tctggttccc aatttacaaa tggcttttct
     2521 atatt
//
LOCUS       DAZUMB010000217         2503 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000217 DAZUMB010000000
VERSION     DAZUMB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.222"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(632..>2503)
                     /locus_tag="ACG80L_002809"
     CDS             complement(632..>2503)
                     /locus_tag="ACG80L_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFS5024986.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          721 a          501 c          395 g          886 t
ORIGIN      
        1 ccaaattgat ctatcaataa ttatcaaact gagtcatata gagacaaaaa tttcaaaaat
       61 gacctgttaa atggtcatcg aatatcattt aaaatcaata cgacggagtc agacacggag
      121 tcaaaacaag cccgctgaga gtaaaaaata tacagtccca taggtaaagt gccacaggac
      181 tgtatatttt tttatttgag attacctgaa atgattccat ttaattttta tctcattttt
      241 tcctactagt tagtatcaaa tactctccta ttttctcaat aaactatttt gggttacatg
      301 gtatctgaaa cagctaaaac cttgtcatta tagcaaaaaa tcactttatc tccgtttcat
      361 ttcagataca tttcacttaa gcggattcca aaaaaacaaa agaaaaagga acccctagtt
      421 agcctatgct ttttattggt tcctctcaaa acacggtcat ataacaacgg ggtggggtaa
      481 atgaatgaaa aaggtggagg aaacattatt caatagctgt cttaaatact tcgttataaa
      541 atgtgaacaa ataaaagaca tctatcccta ttttctacac ttccacttga ctaccatctt
      601 tgaatcgtac aattttctta actcttctcg tttacctcaa ttcattctca agtggaatca
      661 actcatcatc taacatttta cgccatgctc gcttcatttc ttgtgggtat ttaaatggct
      721 tcgcttctgg atgtttggct ttatatcctt cgtaatcctt actgttgata attgctttca
      781 tattaggaat tggatcttca cctggtagat attgcagatt agaagcatgt aattctttct
      841 cagaaacaaa ccactcattg gaaaagacat gaatcaaatc atcactcgct tgtgtaaaat
      901 agcgacgttt aatgacaaag atatcttcat tactatctaa agtccccaca tcaaatgcat
      961 ttaaaatttg tcggtaaact tctttgactc cttctgtttt attcaattta gccaaagctt
     1021 tttcaatttc ttctcgtgca tctgaactat tcgcagcata agttcctagt aattgatcaa
     1081 tataagttga atcaatatca tacaacttag tggtattttc actataaaat tcgatcgttg
     1141 aaaaatcttc tccttctttt tctggttctt cctctattaa tgaacccttc acggtctcgt
     1201 acacgccaat ttgttcttcc aaaagttgaa tttgattgtt caaagttgga gatttcacca
     1261 tgtcatcatt gtaatcgtca taagtcacta acgattcata agcacgattc aattcttgaa
     1321 atgctttgac gtactcgata cggatttcaa gtggcgtatg ttcatcaatg gattccatgg
     1381 atgaaacctc ttttaactta ccatatgctt gtttgaatct ttttttggat tcctgataac
     1441 ttggataaat caaacctgat tcttcttttg cttttgaata cagttttgtc gcatcctcga
     1501 cattcttctc cattgtcgct ggcttacgaa acgtcacaat caatcctttt gtcttatcag
     1561 gatacgtacg attcgtacgt gaaaaagctt ggatcaatcc tgcatactct agattacgat
     1621 ccacaaatag ggtttgaatc gtaggggcgt caaaacctgt taatagacga tccactacaa
     1681 tcgctaaatc gatttgtttc ccaaattgtt tgaattccgc tctttttcgt gccaaacgat
     1741 tattgatgtc tccgttataa cgctcaatgt ctgctaatga ccaggaagta tcataatact
     1801 gattatattc ctcaataatt ttttgcattt cttcttgttg ggcacttgaa tctcggctat
     1861 tttcttctaa tgaataggta atagcgattc gtggaaaatc aggatcctcc atcgttcgtc
     1921 cttcacgaat gagctgattg ggaaactctt ttgttaacca atcaggctct ttcgtcatct
     1981 ctttgatggc ccgataatac tttttggcca tgtcaatgga actcgtggtt aagattgcag
     2041 atttcgttgg cctgccattt cgaaaatcaa attttgtata ggcattatct ggacggaaca
     2101 ttttatgaat gactttttgg atatgttcat ctgcttcaaa aacagctggg tccaaataac
     2161 tttctttttt gacaggttcc atttgatcaa tcatacgatt gatctgttct gatgaatact
     2221 ctgcatatgt ttctacttct tttaatttcc gatagatttt attctctagt gaagtcggct
     2281 ccacggtgtt ttcatgctct acttgaaatc ctaagacaga gccatcttct agcgcattct
     2341 taatggtata ggtatgcaag acctctccat actgatcatg ggttgtacgt gccaattgcc
     2401 ctttggcttg tttccgattc tcaggaaaaa tcggtgtacc tgtaaagcca aaccaagtgg
     2461 atttcggaaa catttttttg atttcttcca tattttcagc act
//
LOCUS       DAZUMB010000218         2488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000218 DAZUMB010000000
VERSION     DAZUMB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.223"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACG80L_002810"
     CDS             191..445
                     /locus_tag="ACG80L_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5024987.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACG80L_002811"
     CDS             446..763
                     /locus_tag="ACG80L_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5024988.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACG80L_002812"
     CDS             1322..2257
                     /locus_tag="ACG80L_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024989.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILITRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2351..>2488
                     /locus_tag="ACG80L_002813"
     CDS             2351..>2488
                     /locus_tag="ACG80L_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS5024990.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          921 a          342 c          457 g          768 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga aacgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttta ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataactc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag gaaaatgtct
     2281 aaacatgtta gtaaatcaac aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg
     2341 aggactacat atgcgttcat caaaatatac tgaacattat acagatactt ttataacatt
     2401 caaggaaatt atgagaactg tttctaacat ataccacaat tgtgtaccag ataagattaa
     2461 aaaccgaaga aatactgatc aactgaag
//
LOCUS       DAZUMB010000219         2483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000219 DAZUMB010000000
VERSION     DAZUMB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.224"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..397
                     /locus_tag="ACG80L_002814"
     CDS             <1..397
                     /locus_tag="ACG80L_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5024991.1"
                     /translation="TSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRENIEKI
                     VSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLRLQGKK
                     SDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            401..1801
                     /locus_tag="ACG80L_002815"
     CDS             401..1801
                     /locus_tag="ACG80L_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFS5024992.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            1824..2198
                     /locus_tag="ACG80L_002816"
     CDS             1824..2198
                     /locus_tag="ACG80L_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5024993.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT          890 a          315 c          500 g          778 t
ORIGIN      
        1 tacatctcat ggaaatattg ccattggatt tgtagataca ttaaaaatgt tttttggcga
       61 taatttaaat tgtcatgtgg taacacttgg agatgcaggc attgaagaat tccgtgaaaa
      121 cattgaaaaa atagtttctc aagtaaaaaa tcaagaagtt atggtatttg ctgacttgat
      181 tggtggaaca cctttcaacg aatttgctaa aagaagtgaa gtatttcaaa atgggtttca
      241 acttcttggt ggcatgaatt taggaatttt gattgaggct gtcaatttga gattacaagg
      301 aaaaaaaagc gatgatatca tcgaaaatct aagaaatatg aatacaattg cgtgtcttgc
      361 agatatgcaa agacatacta acgaagagga tgagtgataa atgcaaaaaa ttttttacaa
      421 gcctaaagat ttttggtttg gggattgcat gcctttttat aaagatggaa ctttttatct
      481 atttcatcaa cgagatacga gaaatcctaa acctttaact gatccttttg ggtggtcact
      541 agttacaacc actgattttg tgaaatttga agaatacggg gaagtaattc atcgtggtgg
      601 caacgatgac attgatcaat acatttacgc tggaagtatt gtccaaggaa tcaatgagta
      661 tttagctttg tatactggac ataatcgcaa agctaagttg gatggaaaaa cttcagaagt
      721 gttaatgatt gcaagttcta aagatttaat tcattggaaa aaggatggcg aagcagcaaa
      781 acttgttcct caagaaggat atgataagaa tgattggcgc gatccttttg caatttttga
      841 tcaagatcat ggaaaatacg ttttgatact aggagctaga aaaccagcaa gtaagaaaaa
      901 gccgacgggg agattggtct attttgaatc cgaagattta aaagattgga atttcaaagg
      961 agactactgg gctaataatg aatttaacat gattgaaatg cctgatatat ttgaaattga
     1021 tggaacgtgg tatttacttt tttcagagta ttgtgaagat aaaaagacta aatatcgagt
     1081 cggagcagga ttattttcag attggaaatc accagtagac gaagctttcg acggaaaagc
     1141 ctattatgca gcaagaacag tcggaactaa tgaaactggc cgttttctaa ctggctgggt
     1201 ggcaacaaaa gaaggggaga acgatcaaaa taactgggat tggggcggtg cacttgttcc
     1261 acatcaaatt attcaaaaga cagacaaaac tcttggtgtt aaactgcctg aaaaaataat
     1321 tgagaatttt aaagaaatca ctccattttc aagttttaag ttagagaaag aatttggaaa
     1381 aaaagaaaga gtaattgttc ctaaaatcga agattatttt ctatttgaaa caactttgac
     1441 tattacatcg ttaacaaagg agtttgcttt aaaattattt gtagatagtg atactcaaga
     1501 aggatacgaa tttcgtattt cattgattaa taatcagctt actttcggaa aaactccgaa
     1561 ctatccttgg cctcaaatgt ttgataaggg gctgcaacga ccactatttt tggaaatagg
     1621 caaagaatat gaactaaagt taattgttga tgatacgata gctgttttat atattgataa
     1681 tgttgcttta agtactagaa tgtatgataa agtcggaaca gcattaagta tgactgttac
     1741 ggagggagca atcaaggtag ataaactaga ctattcaata aattatgaat gtggatttta
     1801 aaacaattga agggatgatg taagtgaaat taaatggttt acaacatatt ggaattccaa
     1861 caaaagattt tggagcttca aaaatctttt atgaatcctt agggtttagt ctgattaatg
     1921 aagaaatgaa tgtgggaagt aaagtcggct ttttcgagtt gaatggaacg ataatagaaa
     1981 tatgggaaga taaaactact gagtgtagag gagccattga tcatctagca ttagatacgg
     2041 atgacataga tgaggtattc aattcaattc aagatttagg atatgaatta ttagataaaa
     2101 atattatgga attaccgttc tgggaaaatg gaatccgtta ctttaatttc tacggaccaa
     2161 atcatgagat tatagagata agccaaatga agaaataaat agataacgaa gtgctctaag
     2221 ttggtatgaa cgaaaagtac ttcatttttt ggtatcagca tttcaatagt ttaatgagaa
     2281 tttactattt caaattaatt caaagtgaca gtatttttta cttatttggt ttcaaatttt
     2341 gtaatccttc ctatttattg gaatacactc cacattttgt atacaagtca tatcatgttt
     2401 tagcaaagct cttaccaagc aaagttataa tgatgttttc caaagtttga tctttcgatg
     2461 gaggctaaca catatttttt gca
//
LOCUS       DAZUMB010000220         2433 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000220 DAZUMB010000000
VERSION     DAZUMB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.225"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..>2433
                     /gene="esp"
                     /locus_tag="ACG80L_002817"
     CDS             107..>2433
                     /gene="esp"
                     /locus_tag="ACG80L_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS5024994.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          881 a          379 c          493 g          680 t
ORIGIN      
        1 ttagttaaca taaggttagt aagattccta ttctgtgaat aatacgtatc agatttatct
       61 tttattctta ctaggaaagt aattattaag ggagttgatt tgaataatgg ttagcaagaa
      121 taataagaga gtatttctag aaaaaacaaa gaaacgagta ttgaagtaca gtatcaaaaa
      181 attaagtgtt ggggttgcat ctgttttgat tggtgttggt cttgtcttgg gaacaactga
      241 actagttaaa gcacaagatg aaatttctcc aagcagccca cttgaaacag cgataagctc
      301 cgtacaagta ggtgacaaag tagcttcggg gaatactttt caggagaatc ctggttatac
      361 gaaaaattat aacttttcgg atttacaatt tagtcctcaa gaactaactg gagacacact
      421 aaaaggcaat acaattggat ttgaagttta cggaaaacat aatattgcag catcaactaa
      481 aaactgggaa atccgtcttc aattagatga acgattagct aaatatgtag aaaaaattca
      541 ggtcgatcca aagaaaggaa ttggatcttc tagacggacc tttgtaagaa ttaatgattc
      601 gctgggtaga cctacaaata tttggaaggt caattatatt cgtgcaagtg atggattatt
      661 tgctggggca gaaacaaccg atacgcaaac cgctcctaat ggggtaatta cgtttgaaaa
      721 gagcctagat gaaattttta aagagatagg catagacaat ctcaagaccg atcgtttaat
      781 gtatcgaatc tacttggtaa gtcatcaaga cgatgataaa attgtaccgg gtattgatag
      841 tacgggctac tttttaacag attcagatga tttctataat agcttagatg tatctgaaaa
      901 taatcctgac cagtttaaac atggatcagt aagtgcaaaa tatgagaaac ctaatactca
      961 aacaaaagat ggttcaggat ccacaggtgc taatggagct ataatcttag atcataagtt
     1021 aacgaaaaat tataatttct catattcatc ttccgcaaag ggcacgcctt ggtatgctaa
     1081 ttacaaaatt gatgaacgat tagttccata tgtagcgggt atacagatgc acatggttca
     1141 ggctgacaaa gtaacatatg atgtttcgtt tgaatccgga acaaaagtag ctgatttagc
     1201 aatcgagcga cgggaaggtc atgaaaatta tggcataggt tcaataacta ataatgattt
     1261 aactaaactt atcgattttg ctaatgcaag tccacgtcca gtcgttattc gatatgtttt
     1321 acaattgact aagccattag atgaaatctt agaagatatg aaagcaacag cacaagttga
     1381 agaaaataaa ccatttggtg aagatttcat ctttgattct tggttgtcgg atacgaataa
     1441 aaaattaatc cagaacactt atggaacagg ttattattat ttgcaagata ttgatggtga
     1501 tggaaaccct gacgataaag aagagagcgg agacacgaat ccatatatcg ggaaacctga
     1561 attagaagaa gtatatgatg ttgacacaac agttaagggg aaagtattca tccacgagtt
     1621 agcgggaaca ggtcacaaag cccaacttgt tgataaagaa ggtactgtat tagcagaaaa
     1681 aactatcgct ccaaatgaaa aagatggggc tccaatttca gatactgtag aatttgaatt
     1741 tacgggtgta gattcaagta aattaatcgc gaaagatgaa ttaaaaatcc aaatcgtttc
     1801 tccaggtttt gataaaccag aagaaggttc aaccgttatt aaggaatcac caaaagcggt
     1861 tgataaacaa accgtggtag ttggatttaa accagatgct aaagaatcaa ttcggaataa
     1921 taaaaactta cctgaagatg cagagtattc atggaaaaca gagcctgata cttctaacgt
     1981 tactgatagt acgaaaggta ttgtaactgt taagatcgga aatcgaactt tcgacgtgga
     2041 tgtagagttt gctgtaaaag cttctcaagc tatggaaaat gatgcaacat acgtacctat
     2101 aacaacaact ccagaaacga cagttcaaag tggaaaacct acatttgata aaccagatgt
     2161 tcctttagct aaagatgcct tttcaatttc agatgtctat aataagtact tcggtaatgc
     2221 aagtgttgat gcaaacactg gtattgttac attcactcca gctaaaggtg taggagaatc
     2281 ggagccgatt actggaacaa ttcctattaa aattgtttac caagatggtt ctgtaggcac
     2341 gaccgattta gcagtaactg taagtaaaga tatttatgaa aatccaggag aaaacattcc
     2401 tgcaggctac cacaaagtaa ccttcaccgc agg
//
LOCUS       DAZUMB010000221         2410 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000221 DAZUMB010000000
VERSION     DAZUMB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.226"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..226)
                     /locus_tag="ACG80L_002818"
     CDS             complement(<1..226)
                     /locus_tag="ACG80L_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS5024995.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFF"
     gene            319..668
                     /locus_tag="ACG80L_002819"
                     /pseudo
     CDS             319..668
                     /locus_tag="ACG80L_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(940..1230)
                     /locus_tag="ACG80L_002820"
     CDS             complement(940..1230)
                     /locus_tag="ACG80L_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5024996.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1438..2130)
                     /locus_tag="ACG80L_002821"
     CDS             complement(1438..2130)
                     /locus_tag="ACG80L_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5024997.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          703 a          447 c          343 g          917 t
ORIGIN      
        1 aaaagaacat taagaatagt tctataataa ttcttgaact ccatgtctta taatgttcta
       61 tcgaagcacc caaatagcca agttcatcta atctacttgc ataaactacg tcatccgctc
      121 tcatagtcgg catatacata ttaaagagta aaaagatacc ccaaatccct attagtaaca
      181 agatcagttc tttgttattc gttatttttc tattatgttt gttcattttt atactccttc
      241 ttatatttcc taatagagta taacatagct ttaagcatta cttaaagata acttaaaagt
      301 tatcttttaa atacttaatt gttaaatacc ttatttgatg caaaagaaat ctacgatagt
      361 caacaaaaaa attttcatta tatccagaaa aatttttctt agttaaggac ctatggattg
      421 cagtaatgca gaattcgtcg aataaactgc caaagacata caatcatatt gcttttaaaa
      481 ttgacgaaca agatatagat tcgtttgtct caaaaatcca aatgttaggt ttaactgtcg
      541 aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac gactatgata
      601 atcacctctt cgagttacat accggtactc tccaggaacg tttaaaatcc tacaattcca
      661 ctacctgaga ctagtaatga ctagtctctt tttatggcct ctgatccccc cattgagcga
      721 atactaatag ggcttcgaat tgatctgttc aatctttaag caatatccct caaatgagtt
      781 tggaaagttt agtgattcat cacaaatact taaatacatg tagttatata tagattgagg
      841 ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg tctgcatcca tctttccaaa
      901 acaagcggct aggacatttt tgtctcagcc gcttgttttc tagtttattc tttcatcttg
      961 tcggaccttt tttcttgggt atagactttt ctcatattct accacaattt tgggtttccc
     1021 taacacatga taggatctca caacaaattg ctttctatta ttataataac ctgtaacgac
     1081 taacttcatc ccttcgtcta cttcagctag gaagttcaaa ctatgggaag caatcaaaca
     1141 attgacccca tttaaactga aacggatcaa cggtctggca ctaacttgca aagtacggat
     1201 cttatctact gttccatatt ttttctccat aaccttcacc tcaataccat tatacgaaca
     1261 tacattccct ttgtaaagaa gaaactctgt tttatctttt tgactttttg tctattacct
     1321 ttcttaaaat catattggca cttcctgcga agatgaagga catgtactaa aaaaaagagg
     1381 cagcagtata gattactgtt gcttctttac taatttaggt ttacttgtta agaaagatta
     1441 tttacgcaaa tgttcttcaa caatttgctg agatttatca gctgtatctt ccacttcatc
     1501 ttttgctttt tctaactttt gaatatattg tttcaagctt gagttttcag cagttgcttc
     1561 gtctaatttc ttttgtgtat ctgttaactg agtttctaca tttgtttttt cttgtgtcag
     1621 tgttgcgatt tgttgattca aaccatttac agtattttgt aattctgaaa gttttgcttg
     1681 tgcttcttgt agtcttgctg tccaatctgc attcagatga ttgatttcat catttttttg
     1741 gttgatctca tctagtttga cattgatttc attttgtttg ctttgtagtt gtgcatcctt
     1801 gctgactaat tgactttcta agttttgttt ttctgctgcc gattgatttt tttggttttc
     1861 taattcattt acttttgttt ggtattcttg gacatctgcc tttgaatctt gcaatgtctg
     1921 ttctttttgt gctagtcgag cggtaagatc tgtaagcgct tgctctttat ctgtcaatgt
     1981 atgatcaagc tgtgtcagat tctcattgat tgtatcaata tctgtctctc caccccacaa
     2041 aataatttca tttgcgtacc gcgtgcctgt gtagccaatt agacatagaa atgcaacgac
     2101 aatcccacca gtaataaatt tctttttcat ttgcatagct ccttaatcca atacttttac
     2161 atgctaaaag taagtgatct caaacacaat gtcaagcgtt tttaaattac atgtgtaatt
     2221 ttaatataaa tactatcaaa tactcattta atcctccttt tttatctatt taaaaaatct
     2281 tttttataga gaaaaatgca ggttaatact ttcttaaata aatatttatg gtgaacaaaa
     2341 acaaaaaagc aagttttatt aaacgtaacc cttttcattt catagattac tgtatttatt
     2401 acaaaaaacg
//
LOCUS       DAZUMB010000222         2407 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000222 DAZUMB010000000
VERSION     DAZUMB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.227"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACG80L_002822"
     CDS             5..1150
                     /locus_tag="ACG80L_002822"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS5024998.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACG80L_002823"
     CDS             1321..1779
                     /locus_tag="ACG80L_002823"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS5024999.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACG80L_002824"
     CDS             1938..>2407
                     /locus_tag="ACG80L_002824"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS5025000.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAZUMB010000223         2385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000223 DAZUMB010000000
VERSION     DAZUMB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.228"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            220..1833
                     /locus_tag="ACG80L_002825"
     CDS             220..1833
                     /locus_tag="ACG80L_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5025001.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          749 a          424 c          454 g          758 t
ORIGIN      
        1 ttcgatccat catttttctt cagcttttaa aaaatataca gggttatccc cttcaaagta
       61 ttatctatcc ttaaaataga aaaaaatttt taggttttat ccacaaatta tttctctata
      121 ttttctgtct attcaaaata aacaataatt ttccggcaca attatttgct gttttttacc
      181 ataacatagt acgatcaacg tagaaaagga gatgttttta tgaaagttgt tgtcattggc
      241 ggtgtagctg gcggtccttc atttgccact cgtttccgtc gattgaatga agcgcacgaa
      301 atcattatct atgaacgcgg agaaaatatt tcttatgcaa gttgtgcttt gccttattat
      361 ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa
      421 acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa cagcaatcga tccttctact
      481 aagcgattaa cagttaaaga cctatccaca aatgaagaaa taaaaacaga ttacgataag
      541 ttgatcatct cttctggtgc tagaccagat tatccggata ttcccggagt ttttgaagca
      601 gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa
      661 gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg ttatgggtct ggaattagct
      721 gagaatttca agcatcgcgg cttagacgtg actttaatcg atcaattgcc acaagtcgct
      781 ttcccttatg atccagaaat tgctaattta gtttatgaca aattgctgaa agaaggatta
      841 gccgttcatt tagaaacaag agttactgag atccgtgata aaggtcgaga aatagtatta
      901 tcagatggct ccgtcctttc tgctgatatg ctaatttttg ctgttggtgt ttctccgaag
      961 aatgaagtgg tgaaagcaac cggcatacaa ttatcagata caggtcagat cattgttgat
     1021 gaccagttac aaaccaatct gccggacatc tatgcgattg gcgatattat cgaaacgact
     1081 agtgtagtga ctggtcagcc gatccaaagt atgctttcca gtgcggccaa tcgtcaagga
     1141 catatgctgg cagatatttt aaatggtacg cctatgcgct atcgcggata tattggtgca
     1201 ggtgtcgcaa aaatctttga tcatacagca agttatgcgg gaatgacaga acatgaatta
     1261 aaagcatcag gcataacaaa ttataaaact gtttttatca ctccctttga ccatgcctat
     1321 ttctatccag aagctacaag attaaatcta aagctgattt ttgatgcaga tagcggtcgt
     1381 attttaggtg gacaagcatt tggagaaaaa ggtgtcgata aacggatggg agaactttct
     1441 gtagcaatca ccggaaactt gacagtcttt gatttgcctg atttggagtt gccttactct
     1501 ccaccgtatt ctactacccg tgacccgttg aatatagctg gttatgtcgc aatcaatcaa
     1561 atgacgaata tcgtagaaac gatcaaagca agtgatatac ccgaaaacga tttgaaagaa
     1621 gcgttctttt tagatatacg tgaacctaat aaagcacctt ctggcagtat ctcggcaaca
     1681 aaaaatattc caatgaatga gttacgagat cgaatcaatg aaatcccaaa agataaaaaa
     1741 gtttacatta ctttcagaag aggattgaat acttatactt ctgcccgaat cttggcaggt
     1801 ttgggtatca aagcgatttt gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg
     1861 tctaggagtt atttttactt gaggagtaaa aatgaaaaag tatatagggt tgttggttgg
     1921 tatgtatata gaatagaaga taaatgtgaa gaaattgtgc attatttctt cacatttatt
     1981 aggttatagt ttaagaaagt ttaaacattg cgcaatttaa ttattttagc tgtttctttt
     2041 cataaaaaaa catgttgtga ctctgagaca aatcatagtc catagtgatg tctctagagt
     2101 aactacgcct aatattacga ggcatcatat taatctatca gcactagtac ctaaactagc
     2161 atccctttcg aatatttttt cttatataat aaagtttgct ttcactcctc taatctcacc
     2221 aaatcgactg gcttgcgttg tgtgatacta ggaactgatt ggtttagaac gacttttcta
     2281 ttgtaatcat atttttcgcc atcctcgttg tcttctaatc cttcaatgaa tgatccctaa
     2341 tgtgtgtatt aaataattta atttttttgt tcctatttat ttaac
//
LOCUS       DAZUMB010000224         2340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000224 DAZUMB010000000
VERSION     DAZUMB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.229"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..2070
                     /locus_tag="ACG80L_002826"
     CDS             208..2070
                     /locus_tag="ACG80L_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5025002.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2340
                     /locus_tag="ACG80L_002827"
     CDS             2070..>2340
                     /locus_tag="ACG80L_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HFS5025003.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIG"
BASE COUNT          842 a          347 c          458 g          693 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
//
LOCUS       DAZUMB010000225         2336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000225 DAZUMB010000000
VERSION     DAZUMB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.230"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..2185
                     /locus_tag="ACG80L_002828"
     CDS             32..2185
                     /locus_tag="ACG80L_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS5025004.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          802 a          415 c          523 g          596 t
ORIGIN      
        1 gcatgaatgt aaataggcgg aaggatagga aatgatgaaa gattttatga aaaaaatatt
       61 aaatacttct tggttagaaa aaataagaaa aggtgcacaa gaacctaaga acaaaacgtt
      121 tcgccggtcc catgttcctt ttcgcttaaa ttttcttttt ttcattatat ttacgttatt
      181 tgttgcgttg attgctagat tgggttattt gcagatcgtg aacggcgaag agatggaggc
      241 gcgagtgaag tctacttcta cgattacggt ccaagaaagt actcctcgcg gaatgatcta
      301 cgactccaca ggaaaagctt tagttacgaa taaagcgaat caggccatca catatacgag
      361 aggaacacaa gttacggcta aagatttgct tgaaatcgca acgaagctga actcgctgat
      421 cgatgttccg gtagatgaaa atctgacaga tagagataaa aaagattttt ggctggctga
      481 tcccaaacat ttgaaagaag ccacaaaaag actttcggca aaagaaaagc aattagggaa
      541 cagcgaacaa tatagtgcga tggtcaataa agtcacagct gatgagatta atttaaacga
      601 ggagcaatta aaaattgcta ccatctttaa acggatgaat agtgcatatg aattaaacac
      661 tgtctatatc aaaaactctg gtgttactga tcaagagtta gctgttgtgg cagaacgcgc
      721 ttctgaactt ccaggagttt cgactggcac agactggaca cgagaatata atgcagcaag
      781 ttcactgaaa agtattcttg gcagtgtgac gactgaaaag caaggacttc cagcagatga
      841 agcagagaaa tacctagcaa aaggttattc tcggaatgac cgtgtaggac aaagttattt
      901 ggaaaaacaa tatgaagatg tcctgcaagg aaccaaaacc caatatgaag tttcccttga
      961 taatgaagga aatgtttcga atcaaaaaga aatcttctct ggggaaaaag gttctaattt
     1021 aatgttatct atgaatgcag aattccaatc aaaagtagaa gaaattttga aaaggaatta
     1081 tcaaacattg atcaacaacg gcaaagcaca gtattcgcct ggtgcttatg cagtggctat
     1141 gaatcctcag acaggagaag tgctagcaat gactggtttc agtcatgaac aaggttccaa
     1201 agaaatcacc gagaatgctt taggcacgat tactagcgcc tttgatccag ggtctgtagt
     1261 caaggctggt actttgacag caggctgggc aagtaacgcg atcagcggaa atcaagtttt
     1321 gattgatgag ccgattcgtc tgcaaggtgc ttcagaaaaa agctctgtct ttaatcgaag
     1381 cggacaagtt gctcttgatg cagtcaaagc atcggaattg tcttctaata cgtatatgat
     1441 caaagtggcg ttaaagatgt taggactaga ctatacacct ggaatgggac tacctagttt
     1501 agatgaagaa gctaaagctt atcagcaatt gagagactct ttcaaagagt ttggtcttgg
     1561 tacaacgact gggatcgatt tgccgaatga atctcctggg atctcacgtt ctgttgatta
     1621 tatgaagaag ttcaatgcgg ataatggcaa agaatggtat gctccaggta actttaccga
     1681 tttggcattt ggacagtttg acacatatac accaatccaa ttagctcaat atgcttcaac
     1741 agtcgctaat ggcgggaaac gagtacagcc gcacctagtc aaagcgatct atggcaatga
     1801 cgaaaatgga aacttgggtg aagtgaagaa agaaattggc acaacagtag aaaatacggt
     1861 caatatttct gcagaaaata tggcgatcct ccgtgaagga ttccaccaag ttgtccatgg
     1921 taccgatcct tacacaacag ccaaaccatt agccagtgcg aaaatggacc tttccgcaaa
     1981 aacagggact gcagaaacgg ttgcggaagg tcatccagat attgcgacag tcaatagtaa
     2041 tatcgtggca tacggaccaa cagacaatcc ggaaattgcc atcagtgttg tcttgccgaa
     2101 cttattagac gaacaagacc acataaactt gacgattgct aaagaaataa tggacgcgta
     2161 ttatgatatg ttcatggcaa aataaaaaag aggtcgtgaa aaagccgctt agtttcgagt
     2221 atgaagaaaa attttgtgaa aatagcttct cacatttttg cttaaatttg acttcatacc
     2281 gagaaactgg cttttgaaca ccgtttactc ggcaaaaaag gttgtgacaa aaattg
//
LOCUS       DAZUMB010000226         2314 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000226 DAZUMB010000000
VERSION     DAZUMB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.231"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACG80L_002829"
     CDS             complement(<1..426)
                     /locus_tag="ACG80L_002829"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5025005.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDG"
     gene            complement(436..1227)
                     /locus_tag="ACG80L_002830"
     CDS             complement(436..1227)
                     /locus_tag="ACG80L_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5025006.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(1220..1993)
                     /locus_tag="ACG80L_002831"
     CDS             complement(1220..1993)
                     /locus_tag="ACG80L_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5025007.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(2149..>2314)
                     /locus_tag="ACG80L_002832"
     CDS             complement(2149..>2314)
                     /locus_tag="ACG80L_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFS5025008.1"
                     /translation="DGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYEL
                     IYTIFEQTIF"
BASE COUNT          740 a          474 c          323 g          777 t
ORIGIN      
        1 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
       61 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
      121 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
      181 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
      241 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
      301 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
      361 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
      421 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
      481 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
      541 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
      601 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
      661 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
      721 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
      781 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
      841 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
      901 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
      961 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     1021 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     1081 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     1141 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     1201 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     1261 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     1321 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     1381 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     1441 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     1501 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     1561 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     1621 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     1681 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     1741 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     1801 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     1861 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     1921 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     1981 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     2041 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     2101 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     2161 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     2221 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     2281 tccctctttc aaatgttcat ttgttattcc atca
//
LOCUS       DAZUMB010000227         2275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000227 DAZUMB010000000
VERSION     DAZUMB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.232"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            263..880
                     /locus_tag="ACG80L_002833"
     CDS             263..880
                     /locus_tag="ACG80L_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5025009.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            921..1220
                     /locus_tag="ACG80L_002834"
     CDS             921..1220
                     /locus_tag="ACG80L_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS5025010.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1254..1421
                     /locus_tag="ACG80L_002835"
     CDS             1254..1421
                     /locus_tag="ACG80L_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5025011.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1466..2071)
                     /locus_tag="ACG80L_002836"
     CDS             complement(1466..2071)
                     /locus_tag="ACG80L_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5025012.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          758 a          366 c          425 g          726 t
ORIGIN      
        1 catggaagac ataaaagaaa aactggataa ctagtttatt taatggcaga ccgttaaggc
       61 ctgccatttt tatgtatttt agcggaataa atataatcat gcgggagtgt aaacttactg
      121 tttcaaaaag tatacttttt gacatgcctt gattttcttt gtttatgtat ggcataataa
      181 ccacataata caatttgaaa cgtttctgat ttaatctgat tgttttgaaa caattttagt
      241 gaaaaaaggg tagataaaaa cgatgagaaa atttttttat atccgggtca gtagttatga
      301 tcaatcgatc gaacgacaac tgatttccgc gaaggaatta ggaatccctg aagaatatat
      361 atttgtagaa aaagccagcg gcaaagattt taaacgccct gaatatcaat taatgaaacg
      421 gatgtttcga aaaggagatt tactctatct acaatcatta gatagactag gcagaaataa
      481 acaaatgatt ttagaagaat ggcgagaatt aactaaggaa aagcagattg atatttctgt
      541 gttagacatg cctctgttag atactgctag ttacaagcaa ctagatgggt tagagacgct
      601 tatctctgac ttaattctgc aattactcag ctatatggcc gaagacgagc ggaaacgaat
      661 taatagccgg caaagagaag gcgtagaagc tgcgaaacaa aagggtgtaa aattcggcag
      721 aaagaaaatt gctttacctc ctgaatttcc tgcgatctat tctgactgga aagcaggaaa
      781 gataactgca gtctatgcta tggatcaatt gggcatgaaa cgaaatacct tttatcgacg
      841 ggtgaaagaa tatgaacata ccttgcacca cttttcttga tagatataac agctattaga
      901 cgggaaggga gagtttgaaa atggacttaa cgaatcaaag ggtcgttttt actggaacgc
      961 tgcagcaaat gacacgaacc caagccatcg gtttggttca ctctttaaac ggccattgtc
     1021 aatcaagtgt tacttcaaaa acaaatttcc tggtttgtgg tcagtattct aagtcgttgt
     1081 ttgatgattc tctctataca aaaaaggaac aaaccgcgcg ggacttgatt gcgttaggtc
     1141 atgagattac cattttatca gaagtcgaat tttatgcgtt aattagtcaa cagttgaaag
     1201 aatggcaacg gtatttataa tcgttagtaa aatttacaag gaggaaaatt tttatgaagc
     1261 gaaaatctta tgatcgttgg ggcattgaaa taactagttt taatcgaaat attgcttttc
     1321 acgtggagtg tgactgtggg gaattagcaa aaagtgttta tcaaaaaaat tacttttact
     1381 gtcctaaatg ccatcgccga tacggggtac atggaacata gaaacgaaat agtgcggtag
     1441 aaaagcgaat gaatgtagta tatttctaca aatcattcgc tttctttatt ttataaatag
     1501 ttggtcgaga gatcccgttt tcttttgcaa tctctgcaac tggaatgcct tgctttaaca
     1561 tgtccaccac ggttttaaaa actaagcgtt tttgcggatc tgttgagtta atagaatact
     1621 cgattggtcg tcccttatat cggccggctt tttgagccaa taatactcct tgccgctgac
     1681 gttcacgaat tttctcacgc tcgttttgtg caatataact tagcagtgat aaaaacatat
     1741 cgaacatagc ggtatccagg agttcatttc cagtattaaa atctaaaaac tgtgcatcta
     1801 atatttttaa cgctactttt ttctgtttca taaaagcaac tgtatttttt atatcttcat
     1861 aatctcttcc taaacgatct agtgatgtaa cgatgacgca atcaccttca cggatataat
     1921 ttagtaattt ttgaaactca ggacgctcag tatctttacc actggctttt tcttggaata
     1981 atttatcaat gttaagtcct tccatcattg ccaattgtcg atccaaattt tgatcttgtg
     2041 aagatactcg aatatagcca atttttccca taaaaaactc ctttttaaaa gtgatataat
     2101 ttacatgtaa aatttacttg tagttttact gctatcatag catttttaaa aataaatttt
     2161 atgtagactt aaaatttact tcatggaggg tttaaatgga tgaaatcaaa agtaacaaaa
     2221 aagcatttgg catttattta ggagaaccta gtacagaaca attaaaccaa ttttt
//
LOCUS       DAZUMB010000228         2272 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000228 DAZUMB010000000
VERSION     DAZUMB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.233"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..526
                     /locus_tag="ACG80L_002837"
     CDS             8..526
                     /locus_tag="ACG80L_002837"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5025013.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            654..1337
                     /locus_tag="ACG80L_002838"
     CDS             654..1337
                     /locus_tag="ACG80L_002838"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS5025014.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1646..1719
                     /locus_tag="ACG80L_002839"
     tRNA            1646..1719
                     /locus_tag="ACG80L_002839"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1680..1682,aa:Arg,seq:tct)
     gene            1878..2237
                     /locus_tag="ACG80L_002840"
     CDS             1878..2237
                     /locus_tag="ACG80L_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS5025015.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          762 a          353 c          456 g          701 t
ORIGIN      
        1 gaaaacgata cctgattaca aaaaaataac ttttataagt tctataacgt tgattatatt
       61 tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga ctccttatat
      121 tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat atgtacgacc
      181 gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa attcaggaaa
      241 aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt tttatacaaa
      301 agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata ttatcggtag
      361 tcccatcttt cagattccat atattggact agtcctttca aaaacggcat ttgcatggat
      421 tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat ggaatacctt
      481 tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat gttaaaaaca
      541 taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa aacactcatg
      601 cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt agtatgcgca
      661 atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg cgtacccagc
      721 aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta gcagaagtac
      781 cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag cttcagcctg
      841 aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg gccacgacaa
      901 tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc gagaaagcta
      961 aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa ggtcaagcag
     1021 cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat agtgcaaaat
     1081 caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt ggcgttttga
     1141 tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag attccccgtg
     1201 gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg gatcatcccg
     1261 atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca aaggaatcag
     1321 aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt gaatcgaggg
     1381 ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag gatacaggaa
     1441 atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat tgataacctt
     1501 agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg ggccaaaata
     1561 attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt aattatctat
     1621 aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag catccgcctt
     1681 ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg ttagaaactg
     1741 aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc aaaggcaaat
     1801 aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata aaactcatca
     1861 gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta gttgcaattg
     1921 aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg acaagtaggg
     1981 tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg tttaagaacc
     2041 aaggagtgta caatggacta ttaggagtgg gactacttta tggagcttat ctttcttccg
     2101 catctaaaga aatcacaagt atgctattaa tatctatttt ttttgttgca ttatatggca
     2161 gtttgactag cgatcgttgg attttactca aacaaggtgg gctagctctg ttggcattgc
     2221 tttctatttt cttataaata tagaaaaaat aagaatttat ctaacaggaa ac
//
LOCUS       DAZUMB010000229         2261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000229 DAZUMB010000000
VERSION     DAZUMB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.234"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG80L_002841"
     CDS             278..820
                     /locus_tag="ACG80L_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025016.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACG80L_002842"
     CDS             976..1425
                     /locus_tag="ACG80L_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS5025017.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2261)
                     /locus_tag="ACG80L_002843"
     CDS             complement(1455..>2261)
                     /locus_tag="ACG80L_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFS5025018.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          784 a          372 c          429 g          676 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcgatcgta taggtagatg tcaatccgcg gcgtttcatg
     2101 attcgaccga ttcggcgccg actgagttga agcccacgct ttgctaaaca cttcttcaat
     2161 tttcgggtac cgtaagcctt tcggtttcga ataaactctt cctgaacgat ctcttctaag
     2221 tcggactcat tttcgatcgg tttcgcttga taatagtagg t
//
LOCUS       DAZUMB010000230         2258 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000230 DAZUMB010000000
VERSION     DAZUMB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.235"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG80L_002844"
     CDS             complement(<1..182)
                     /locus_tag="ACG80L_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5025019.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG80L_002845"
     CDS             517..951
                     /locus_tag="ACG80L_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5025020.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG80L_002846"
     CDS             974..1294
                     /locus_tag="ACG80L_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025021.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG80L_002847"
     CDS             1435..1626
                     /locus_tag="ACG80L_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025022.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG80L_002848"
     CDS             complement(1697..1954)
                     /locus_tag="ACG80L_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS5025023.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1981..2124
                     /locus_tag="ACG80L_002849"
     CDS             1981..2124
                     /locus_tag="ACG80L_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025024.1"
                     /translation="MIQNNTLCEMAQAVLWIYPVKSVSILKLEEKLAIIKVKEGVGFS
                     WIM"
     gene            2061..>2258
                     /locus_tag="ACG80L_002850"
     CDS             2061..>2258
                     /locus_tag="ACG80L_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025025.1"
                     /translation="MRGETCYNQSERRSGIFMDHVVLEIDLHLINNIRVIYYVVSNSV
                     EQRVLTNKINGILAKKDVHRFN"
BASE COUNT          860 a          328 c          462 g          608 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaat
//
LOCUS       DAZUMB010000231         2232 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000231 DAZUMB010000000
VERSION     DAZUMB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.237"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..543
                     /locus_tag="ACG80L_002851"
     CDS             <1..543
                     /locus_tag="ACG80L_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5025026.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(899..1471)
                     /locus_tag="ACG80L_002852"
     CDS             complement(899..1471)
                     /locus_tag="ACG80L_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5025027.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1487..2092)
                     /gene="fic"
                     /locus_tag="ACG80L_002853"
     CDS             complement(1487..2092)
                     /gene="fic"
                     /locus_tag="ACG80L_002853"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS5025028.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          453 c          338 g          740 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
      661 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
      721 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
      781 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
      841 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
      901 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
      961 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1021 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1081 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1141 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     1201 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     1261 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     1321 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     1381 tcttctctac gcagttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     1441 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     1501 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     1561 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     1621 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     1681 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     1741 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     1801 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     1861 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     1921 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     1981 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2041 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2101 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     2161 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     2221 ttgcacagtc ta
//
LOCUS       DAZUMB010000232         2217 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000232 DAZUMB010000000
VERSION     DAZUMB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.238"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..529
                     /locus_tag="ACG80L_002854"
     CDS             <1..529
                     /locus_tag="ACG80L_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5025029.1"
                     /translation="GSNQIDDYVGKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPI
                     DFAKDLRKEVKPLLDFLKKRDIPLALASSSPVVEIESMLIQCELTEYFSFVISGESLK
                     QSKPHPEIYLKAKQQLDCDFFIAVEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQ
                     IESLQELEQIVLEKG"
     gene            1428..>2217
                     /locus_tag="ACG80L_002855"
     CDS             1428..>2217
                     /locus_tag="ACG80L_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5025030.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWL"
BASE COUNT          722 a          361 c          434 g          700 t
ORIGIN      
        1 aggatcaaac cagattgatg attatgtggg gaaaacagaa gctgcaatat gggaaacatt
       61 agttcctgag gacaaatcat taagagagga actttatccg aaatatgtag attatcgaaa
      121 aacgcatcca attgatttcg ctaaagattt acgaaaagaa gtgaaaccgt tacttgactt
      181 cctcaagaag cgggatatac cattggcact ggcatcttct tctccagttg ttgagataga
      241 gagtatgctc attcaatgtg aacttactga atatttttcc tttgttataa gtggtgagtc
      301 attaaaacaa agcaaaccac atcccgaaat atatttaaaa gcgaaacagc aattagactg
      361 tgacttcttt attgcagtag aggattcaac gattggtatt aaatcagcaa aggcagcaaa
      421 tatctatacg gtggcactaa aacaaccaat gcaaatcgat cagagtgaag cagacataca
      481 aattgaaagt ctacaagaac tggaacaaat tgttttagaa aaaggttagt gaattaatgg
      541 aaataaggct atatatctgt gctagctatt ttgactaacc aaaaaaactt gctataattc
      601 aggtgtagta aaaggtgctg gtaacacctt ttacgaaaat gcttcggcag tgataatggg
      661 attggctttt taagtttttt attttgctcg caatccctaa ggtcatgtga atgatcctag
      721 ggattgctag aatattttgc aacattctat ctatttcaga acgtaacctt ccccagttca
      781 ttgggattca ttagcataca accaacattt caaaaagaaa ttgcactaca ccattattag
      841 ctattttgac taaccccaaa aaacttgcta taattcaggt gtaggtaaaa ggtgctggta
      901 tcacctttta cgaaactgct tcggcagtgg taatgagtgt ggcttaaagg actagtcctt
      961 taagcttttt actttgttcg caatctctaa gatcaagtta atgatcgata agattgctag
     1021 aatattttgc aacattctat ttcagaacgt aacctttccc attccactgt gattcattgg
     1081 caaccctcct cacaaccaac gtttcaaaag aaattgtaaa gttgttgggg taccacgacc
     1141 gaacaatact acacctgaat gatagtaagt tgtctcatta agtatagcat atcctctttt
     1201 tgggtaaata gaaatcggtt acatttttta tccaagacca agttttttca tttggtctta
     1261 tttgctatac ccaaacaatt ccgcagtata gtcgacggta ttgattttat caggtgagta
     1321 atagaaactt gttttccttc cccaaaacat tacctataaa ggagaaaaaa tggtatcgta
     1381 attatatagg tataggatga aatttttttc taagaggagg gttgttgatg aaaaatttac
     1441 agttaaaatt tattgttgat ccatcgattg tacgctggtt tcggattatc aatcaatttg
     1501 agcgcaatca acttgccaca aaaggagaat tagcaaagag taatcatgta tctgaacgaa
     1561 ccattcaaac agatgtcaat aaaatgaagg actacttttc tgaaagtgtt caatttgttt
     1621 caacgaaaag tgggtattct tttcagaagg aaagaccggt tctttttctc aagcagaagg
     1681 agcaactact ggagaacgaa attttatttg agttgttagg gaatgtcttt tatggcgaat
     1741 tggaagatat gacggagcta attgatcgtt ttcatttatc tgaagcgact tttcgtcggt
     1801 atttgaagca gattcaacca gttttggaag aatacggctt agaattgagt ctttatcctc
     1861 ttgatttaaa aggagaagag gcaaatattc gcaagttttt taaagacttc tattatgagg
     1921 gggagatgac gccccatact cttgttccac caagggatct tcatgaactt gtgcaagcta
     1981 cattctatga taaatttgag tatttctcga ttggaacagg gacttctcct gctgaatttt
     2041 attatagttt gtatattgcg attgagcgtg tgagacaagg aaaaacgata gccattcctc
     2101 aaggtctgat tgagcgagta attgctagtg agaattttcg cttaatgtat tccttgaaag
     2161 aagcgattca aacaaattat caagttgctt tatcagaaga ggagttttgt tggttgt
//
LOCUS       DAZUMB010000233         2056 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000233 DAZUMB010000000
VERSION     DAZUMB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.239"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..337)
                     /locus_tag="ACG80L_002856"
     CDS             complement(<1..337)
                     /locus_tag="ACG80L_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS5025031.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="ACG80L_002857"
     CDS             complement(404..559)
                     /locus_tag="ACG80L_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFS5025032.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="ACG80L_002858"
     CDS             1176..1397
                     /locus_tag="ACG80L_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025033.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="ACG80L_002859"
     CDS             1398..1655
                     /locus_tag="ACG80L_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025034.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2056)
                     /locus_tag="ACG80L_002860"
     CDS             complement(1791..>2056)
                     /locus_tag="ACG80L_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005727 - extrachromosomal circular DNA
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rep family protein"
                     /protein_id="HFS5025035.1"
                     /translation="DIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLI
                     DYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcac
//
LOCUS       DAZUMB010000234         2041 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000234 DAZUMB010000000
VERSION     DAZUMB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.240"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2041
                     /gene="acm"
                     /locus_tag="ACG80L_002861"
     CDS             <1..>2041
                     /gene="acm"
                     /locus_tag="ACG80L_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS5025036.1"
                     /translation="HVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQNIQ
                     PGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDSVE
                     GWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDMLPE
                     DTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEEGI
                     QQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSEAW
                     FKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELRRA
                     DEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSINE
                     NDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETKRL
                     DNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKEVI
                     SVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRDGQ
                     EIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKIRN
                     LFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIG"
BASE COUNT          802 a          336 c          402 g          501 t
ORIGIN      
        1 ttcatgtcta tgcggatgca ggcagagata tcagcagtaa tgtcacttcg ttgaccgtgg
       61 acccaacgaa tattacagat ggaggaaaca taaaagtaaa attctcgttt gacgaaaaaa
      121 aacaaaatat ccaaccaggt gattatcttt ggatcaattg gccaagtgaa ggaaatattc
      181 gcggtgaagg attccaaaaa gaaatcccat taatgattga aaataaaaat gttggaactt
      241 taacggtcag aaaagattct gcgcaggttg tgtttaatga aaatattaaa aacctagact
      301 cagtagaagg ttgggggcaa tttgagatac aggccagaaa cgtaaccgat accggtgaag
      361 aaaatacggg accattcacc gtgacgagcg gtgataaaac agctactgta aatgtgacaa
      421 agccagcttc tggttcttct tctagcgttt tttattataa aacaggagac atgctacctg
      481 aagatactaa gcacattcga tggtttttga atattaacaa taacggcact tatgttgaac
      541 aaccagttaa aatcagtgat gaaatccaaa gtggacaaag attagatcca agtacgtttg
      601 aaattaatca aatacactta ggtgaacaga aggtttatag aggagaagag ggaatccaac
      661 aatttttaca agatttcccc agcgctacat ttaatttttc agtaactgat aattacattg
      721 aaataactat accaaaaaac tttgtcaatc ttaggaaaat aatggtgagt tataagacga
      781 tcatagaaaa cccagaacaa ataaattttg agaatcattc ggaagcttgg tttaaagaat
      841 ttaataaacc tgctgttgat ggagaaagtt tcaatcatac agttaaaaat atttcagctt
      901 ctggtggagt caacggtaca gttagaggcg aattgaagat tttcaaatat atcaatgaca
      961 cagagattgg tatcccaaat gtgactttcg aattaagacg agcagatgaa cagcctattc
     1021 aaggacagtc tagtatcctg ttgacttcga atgaacaggg agaagctagt atcaaaggcc
     1081 tgcaagtagg agactatgtt gtaaaagaaa aagaagcacc caattggatt gattttgatc
     1141 cactaagttc aaatgaactc aaattctcca taaacgaaaa tgatacagag ggagtcagtt
     1201 tacctattta taacaagaaa aaggttacga acatcacagc aactaaaatt tggaacggtg
     1261 gaaccactcc aagaccgagt atttatttta aattattcag agcaagtaca aacaattggg
     1321 aaccagttcc tgatgcagaa acaaaacgat tagacaatgg aataacttca gtgacttggg
     1381 aagatatcca acagtatgat gatagtggaa acgaatatac gtttaaagtg caagaagtag
     1441 atcaaaacgg aaatgattat gtcccttcag gttatcaaaa aattgaaaac gggctagtcg
     1501 ttacaaatga gaataaagaa gtcatttcag tctcaggaca aaaaacatgg gaagacaatg
     1561 agaaccaaga cggcaaacga ccgacgacga tcacagtaaa tctattagcc gacggtcaac
     1621 cgatccaaca caaagaagta agtgaaaaag atgattggcg ctatcgtttc acaaatctgc
     1681 caaaatacag agacgggcaa gaaatcatct atactgtaac ggaagacaac gtgcccgaat
     1741 acagtacgac gattgacggg tataacatca aaaacagtta tacaccggaa aaaacaaata
     1801 tatctatacc aggtagccat ataacaccgt ggaaaaattt tccagagaag acagagagag
     1861 agaaaattag aaatctattc tctcaaaaat tgcaaatatt tgctcataca ggagaatcca
     1921 gcgaacaaag aagcaattat attagtaaga gagctgtacc taaacagata acaaataaat
     1981 caaaaagcat tcttcctaaa acaagtagta aaaaatctag ttttgctgta atcattggtt
     2041 t
//
LOCUS       DAZUMB010000235         2005 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000235 DAZUMB010000000
VERSION     DAZUMB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.241"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG80L_002862"
     CDS             complement(<1..914)
                     /locus_tag="ACG80L_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS5025037.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG80L_002863"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG80L_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS5025038.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          662 a          368 c          393 g          582 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaa
//
LOCUS       DAZUMB010000236         2003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000236 DAZUMB010000000
VERSION     DAZUMB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.242"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..211
                     /locus_tag="ACG80L_002864"
     CDS             <1..211
                     /locus_tag="ACG80L_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5025039.1"
                     /translation="LFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFE
                     NVVTGKENWNDLYHANEVLKIAEEK"
     gene            212..964
                     /locus_tag="ACG80L_002865"
     CDS             212..964
                     /locus_tag="ACG80L_002865"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS5025040.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            969..1916
                     /locus_tag="ACG80L_002866"
     CDS             969..1916
                     /locus_tag="ACG80L_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5025041.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          701 a          266 c          396 g          640 t
ORIGIN      
        1 attgtttact ttagatgaat gtatcaatgt tgacttatta aataataaaa taagttttga
       61 agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat atgtatgaga aggagttgaa
      121 atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat atcatgcaaa
      181 cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta tttgtggtag
      241 agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa agcccctagt
      301 ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac ataattgcac
      361 gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt taaatccgtc
      421 tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga aaattgaagt
      481 gatacgtgat tcattcttac aactagaaag agaagataat atatttgatt taattataga
      541 tttagatttg acttcacctt tacgtgaagt ttctaatctt gaagaattaa tagatgaaca
      601 tctaaaaaat ttagataaag acgtctactt ttcagttgtt tctgcaagac gtaatcccta
      661 ttttaatatg gtaaaaagaa ttgaaaatga agttactata gttaataaat caagatacac
      721 atctaggcaa cagacacctg aagtttttga aatgaatgcc tcaatgtatt cttttaaacc
      781 tgaatttcta aaaaaaagta attatatttt tgatgggcaa tgtgggataa taaaaatgag
      841 agattatcta attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt
      901 tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag
      961 gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag
     1021 ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag
     1081 tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat
     1141 ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag
     1201 ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa
     1261 gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt
     1321 ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg
     1381 tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag
     1441 attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc
     1501 caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata
     1561 agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta
     1621 gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag
     1681 ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga
     1741 ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt
     1801 cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac
     1861 cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca
     1921 tactaagata agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt
     1981 gtaaaatcaa atctgggata gaa
//
LOCUS       DAZUMB010000237         1993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000237 DAZUMB010000000
VERSION     DAZUMB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.243"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG80L_002867"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG80L_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5025042.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG80L_002868"
     CDS             852..1040
                     /locus_tag="ACG80L_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5025043.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG80L_002869"
     CDS             1053..1610
                     /locus_tag="ACG80L_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5025044.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG80L_002870"
     CDS             1642..1884
                     /locus_tag="ACG80L_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5025045.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DAZUMB010000238         1950 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000238 DAZUMB010000000
VERSION     DAZUMB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.244"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..493
                     /locus_tag="ACG80L_002871"
     CDS             <1..493
                     /locus_tag="ACG80L_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025046.1"
                     /translation="NIFERHRIIGTIVCITVIPLLISIGILFKFIPNAMVNQMVHIGS
                     PLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKSRGLIWDGIF
                     GLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNLCKLMNHVIN
                     IYP"
     gene            516..1541
                     /locus_tag="ACG80L_002872"
     CDS             516..1541
                     /locus_tag="ACG80L_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5025047.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            1790..>1950
                     /locus_tag="ACG80L_002873"
     CDS             1790..>1950
                     /locus_tag="ACG80L_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5025048.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          690 a          237 c          401 g          622 t
ORIGIN      
        1 taatattttt gagagacaca gaatcatagg aacaattgta tgtataacag ttataccact
       61 tctcatatcc ataggaatac ttttcaaatt tataccaaat gcaatggtta accagatggt
      121 tcatattggt tctccattga tggttatagc aagtatattt ttattttatg tatttaaaaa
      181 tttttcattt tacagtaagt atataaataa acttgcgaga accagtttag gggtatatat
      241 tttacatgaa aatcattatt tcattgatgg tatgaagtct agaggcttaa tatgggatgg
      301 gatatttgga ttaggcaaaa ggtttttaga tagggatttt tggatttggt ttataattgc
      361 agttgtagtt gtgtgcataa tttgcatggc tatcgatgtt gtaagagaaa aatttgaaag
      421 acagattttt aaaattaaga gtgtaaagaa cctgtgtaaa cttatgaatc atgttataaa
      481 tatataccca taattaataa atgaggagaa aagaagtgaa agttatatca ttcggagaat
      541 tattattgag acttgcagca cccggttatt caaagttatt tcaaagagat agtttggaga
      601 ctacattctg cggaggggaa gctaatgtag cggtatcctt agcaatattt ggatatcaat
      661 cagaatttat aacaaagctt ccggattcag atgttggata tgcggcagct aatgctttgc
      721 gttattttgg agtaaatgtt gataatatta tatttggaga gggacgcatg ggactgtatt
      781 atttagaaaa aggagcagct cagagaccat ctaaagtaat ttatgacaga aaattttctg
      841 caatttcgtt agctgatagt gatgaattta agtggaagga gatcctaaac gatgcaaatt
      901 ggtttcattg gacagggata aatccagcat tgtctgagaa tctctttgag acgtccttag
      961 atgcatgtca ggctgccaaa aaaagaggta taacaatttc ttgtgattta aattatcgat
     1021 ccaagctttg gacatcagag gaagcaaaag aaaagatgga acagatacta ccattggttg
     1081 atatttgtat ttgtaatgaa gaagatgcag aaaaagtgct aggaattaaa gctgagcata
     1141 caaatattaa tagtggagaa ttaaataaag atggctatgt gcaggtggct agggagattc
     1201 acaagaagtt tggatgtagg tatgtagcta caactctgcg tagaagttat tcggcaagta
     1261 gaaatggttg gagtgcaatg ttatacgaag cagaaaaaga taaagcgtat ttttccacag
     1321 aatacaacat acaaattgtt gatcgcgttg gtggtggaga cagcttttct gctggtataa
     1381 tatatgcgtt atgtgaaaat atgtccggac aaaaggctat tgaatttgcg actgcatcta
     1441 gttgtttaaa acatactata gaaggtgatt ttaatcgaac tacaattgag gatgtatata
     1501 atttaatgaa tagcggtgga aatggtcgcg tgttgcgtta gtcaacatat aaagataaat
     1561 cacagtaatt tttatggtga ttatctgtct tagtagtgat tagaataacg tgaaaataaa
     1621 ttgttgagcg tttatactgt tttgaaaaat aaatttaaca tattgaaaaa tacaaatata
     1681 tacgtatatt ataaagcgcc gattgtaaaa ttaagctaga caactaaaac agtcatttgt
     1741 gctacactca aaatagttcc gaccaaagaa ttataaggag tgagtgcaaa taacctatac
     1801 ctatcttaca acggatgaac ttgtaataat agagtcttat ttgaaaatta accaatctgt
     1861 tgctaaaact gcgcattgct tgaatcgttc aagtcaaacg atccataaag tatatctgtt
     1921 cttcaagcaa agaaaatcag ccttagaata
//
LOCUS       DAZUMB010000239         1921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000239 DAZUMB010000000
VERSION     DAZUMB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.245"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACG80L_002874"
     CDS             <1..1008
                     /locus_tag="ACG80L_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025049.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACG80L_002875"
     CDS             1025..1174
                     /locus_tag="ACG80L_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025050.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACG80L_002876"
     CDS             1171..1617
                     /locus_tag="ACG80L_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025051.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACG80L_002877"
     CDS             1619..1756
                     /locus_tag="ACG80L_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5025052.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
BASE COUNT          689 a          337 c          401 g          494 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 g
//
LOCUS       DAZUMB010000240         1848 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000240 DAZUMB010000000
VERSION     DAZUMB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.246"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..714
                     /locus_tag="ACG80L_002878"
     CDS             148..714
                     /locus_tag="ACG80L_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5025053.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            859..946
                     /locus_tag="ACG80L_002879"
     tRNA            859..946
                     /locus_tag="ACG80L_002879"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:891..893,aa:Ser,seq:cga)
     gene            1280..>1848
                     /locus_tag="ACG80L_002880"
     CDS             1280..>1848
                     /locus_tag="ACG80L_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS5025054.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          647 a          277 c          356 g          568 t
ORIGIN      
        1 aaattttttt aattttgcaa aaaaaagtta aagaagactg aaaaaaaatt taaaatataa
       61 cgttttgagt aaaatgtttg caaaaaatgc taaaaatagg gtacaataaa acaacatata
      121 ctgcagtaaa gaagtggggg agttttaatg agaagaaaag tctataccaa ggatcaaatt
      181 ctaaaagctg cttatgatgt tgttgcaaag gaagggttta aaggatttac tgcgagaaat
      241 attgctaaaa agatggggat ttcgacacag ccgatttatc tggaattcaa aaacatggaa
      301 gatttaaaaa atacgttgct ggattctatc tttgaacaac taaaagaaga tgttttccct
      361 attgaacgta ctggtgataa aatcattgat ctcggtttga actatatcta ctttgcaaaa
      421 aagcatcata gtctttacat ggcattgtac attgatgaac atggggacgg aaagaaaatg
      481 catgattttt catacaatta tttccgtgaa atagttaaag aagatgaaag atacaatcaa
      541 ttatcggatg aatatgtcga tgctctgcat actggtacat gggtagcagt gactggctta
      601 gcgtcattga tgtcatcaaa catcatgcat ccaacagaag aacaaatcat tgcactgatc
      661 aaacggacaa ttgactcgat tttagcatta gataatcctg aaataatcag atagtgttct
      721 atcagtatga atttgatcgg taagtccgtt ggctgcaaac tgtcaatgga cttttttgct
      781 aaaaaaagcg atttctgtta tttatctatt agaaataggc ttgacatttc gtgaaaaaag
      841 tttaaaaata gtacttacgg aaggttggca gagtggtaat gcaccggact cgaaatccgg
      901 cgaaccggct aataccggcg cgcaggttca aatcctgtac cttccttata tcaacgatta
      961 gaggttgtga agaagctgtc ctgcatcaag taataagaac ggccaaacaa aaatagcttt
     1021 tcctattttt tcgtttgatt gggcttatta ccgcagatgc aagcttttga acaccgtttg
     1081 ttcggaataa ggggctgtga caagactttt gtcacagccc cttattgttg tacttttaac
     1141 ctacaatatt ttttgattcc ccctgagtac tattttttat aaatgctagt aaatcaatct
     1201 tctttggcct ttttttgata aaaaaaatac tagatgtaag ataaaagtgt aaaaacgatt
     1261 tgttttagga ggggaaacga tgtgtaccgc aatcacttat gcaacgaaag accattattt
     1321 tggaaggaat tttgattatg agatgtctta taatgaagtg gtcactatta ctccaagaaa
     1381 ttatcgtttc gattttagaa aggtaaaaaa tttagataaa cattatgcaa tgataggaat
     1441 agcagctggg gtatccaact atccactcta ttatgaggct acaaatgaaa aagggttgag
     1501 tatggcagga ctgaattttc ctggcaatgc tgattataaa gaattacaag aaggaaaaga
     1561 taacgttgca ccatttgagt ttattccatg gatactaggg caatgctcaa ccatagacga
     1621 agctaaagaa ttattggcta ctatcaatct agtcaatatc gattttagtg aaaaactacc
     1681 cttgtcacct ttacactggt tattagctga taaagaaaaa tcgatagtaa ttgaaagtat
     1741 gaaagatgga cttcatttat atgataatcc tgtcggtgtg ttgacaaata atccgccatt
     1801 tgattatcaa ttatttaatc tgaacaatta tcgctcgcta tcaaatgg
//
LOCUS       DAZUMB010000241         1829 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000241 DAZUMB010000000
VERSION     DAZUMB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.247"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..594)
                     /locus_tag="ACG80L_002881"
                     /pseudo
     CDS             complement(1..594)
                     /locus_tag="ACG80L_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            615..1229
                     /locus_tag="ACG80L_002882"
     CDS             615..1229
                     /locus_tag="ACG80L_002882"
                     /inference="COORDINATES: protein motif:HMM:NF023433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025055.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIERT"
     gene            1258..1518
                     /locus_tag="ACG80L_002883"
     CDS             1258..1518
                     /locus_tag="ACG80L_002883"
                     /inference="COORDINATES: protein motif:HMM:NF023433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025056.1"
                     /translation="MDYQLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDL
                     HRTFAQLPVELQPYAEMILNDVNNGELVIKEGWEFTDYLNEF"
BASE COUNT          630 a          287 c          351 g          561 t
ORIGIN      
        1 cgtgaatttc tttttaaacg actgaggtaa cgtagatgaa attgttttaa gggtttcttg
       61 aaagcctacc gtatctcgtt taacgaaata atagcgtaat aattgacaaa aatgatacgc
      121 agttcgcaat tcttcgttgt agcttaacag ctcattaacg atttctgttt gactaattgg
      181 tcgtttaaac agtcgatgat aatgccgttt gagtgcctcc catgtcgtgg aatctttgag
      241 taagagtttc caatatcgct tgagccgacg gtacttcttc tgattttcag agtgtgaggt
      301 tcgaaaacga ttcatgatct ggacacgtaa ttgattgaag ctgcgattga tgtgttggac
      361 aatatgaaag cggtcagtta cgatctccgc atttggaaag accgttttaa gcaactgaca
      421 atagctggca ttcatgtcca tcactaggta cttgaccttt aaacgggcct ttctaggaaa
      481 gcgcaggaag tattgagtta gtttgtaaag tctgcgatct tcaagggtcg taaatagctg
      541 attggttgtc ccatcaacac aaataaaact catggaacca gaagtggcaa cattgtgtac
      601 ttttctttgc aatcttgtgt acaaaactat tgcaaaacac agcgcataag aatccttaca
      661 aaacagtaga tgaagcaacc caattagatc ttgttatagt tgttaatcag ctcttaacgg
      721 gttacgattc aaaatgggtc aacaccctat acttagataa gtcacttgaa tatcagaaca
      781 taattcaggc tttttcacgc acaaatcgtc ttaatggtcc tgaaaaaccg ttcggaacca
      841 ttcgttatta tcgtaaagca catacgatgg aacagaacat tgaagaggct attgagttat
      901 actcaggcaa acaagcaaaa gggttatttg ttgaaaaatt atataataac ttagaaaagt
      961 tgaacgatat attcgaagaa attaagtccg tatttaatat ttctggcata gaaaacttca
     1021 gtcatttacc ggaggttcca ggggaaatta agcaatttgt aaatctttat aatcaactaa
     1081 tccattgtta tgaatcagcg atgatacagg gattcacatg ggcaaaatcg acttatgaaa
     1141 tccaggatta tgaacctata acagtagtaa tcacagaata tgaatttaat atcttggcac
     1201 aacgttacag cgagatagag agaacctaga gacggaaatg atagtatagc gtatgacatt
     1261 gattaccaat tacctgaagg tagtcgtagt cgtattaatt atgagtattt aaacacaaac
     1321 ttcaaaaaat acatgaatat aaaagaagaa cgtgattcga atttaattca atcattgtta
     1381 aacgacttac atcgcacatt tgcgcaatta ccagtggagt tacagccata tgctgagatg
     1441 attctaaatg atgtaaataa tggggaatta gttatcaaag aaggatggga atttactgat
     1501 tacctcaatg aattttagtg gattcaacca atattaatga ggcattatta cgtgagatgt
     1561 tggatttacg cttaaccgag gtaaacatta atgaatatag tcgatttgat aaattaaaat
     1621 caagtgttaa cgtaggtcat ttcagtggat atatcgagaa aaatttaggt aaaatggtaa
     1681 taccaatcaa ggtaaatatg ctaattgaca gattattgcg tgcattttta ttagaagatg
     1741 tattcgatgt tacagagtac ataaagaatt actttaacta gagtacacct acatcttata
     1801 gtacataaaa aaattcatct tttttagtt
//
LOCUS       DAZUMB010000242         1799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000242 DAZUMB010000000
VERSION     DAZUMB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.248"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACG80L_002884"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACG80L_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5025057.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DAZUMB010000243         1738 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000243 DAZUMB010000000
VERSION     DAZUMB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.249"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1234
                     /gene="aph(2'')-I"
                     /locus_tag="ACG80L_002885"
                     /pseudo
     CDS             1..1234
                     /gene="aph(2'')-I"
                     /locus_tag="ACG80L_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1218..1610
                     /locus_tag="ACG80L_002886"
     CDS             1218..1610
                     /locus_tag="ACG80L_002886"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5025058.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          715 a          133 c          297 g          593 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttattgatga taatatggtt tttgatattg
     1261 ataatttaaa aggatttctt aatgatacca gttcatttgg gtttatagct aaagaaaata
     1321 ataaaattat aggatttgca tattgctata cacttttaag acctgatgga aaaacaatgt
     1381 tttatttaca ctcaatagga atgttaccta actatcaaga caaaggttat ggttcaaaat
     1441 tattatcttt tattaaggaa tattctaaag agattggttg ttctgaaatg tttttaataa
     1501 ctgataaagg taatcctaga gcttgccatg tatatgaaaa attaggtggt aaaaatgatt
     1561 ataaagatga aatagtatat gtatatgatt atgaaaaagg tgataaataa atgaatatag
     1621 ttgaaaatga aatatgtata agaactttaa tagatgatga ttttcctttg atgttaaaat
     1681 ggttaactga tgaaagagta ttagaatttt atgatggtag agataaaaaa tatacatt
//
LOCUS       DAZUMB010000244         1714 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000244 DAZUMB010000000
VERSION     DAZUMB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.250"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..578
                     /locus_tag="ACG80L_002887"
     CDS             <1..578
                     /locus_tag="ACG80L_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5025059.1"
                     /translation="YFERRMNFFQELEIDPGSNQIDDYVGKTEAAIWETLVPEDKSLR
                     EELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIPLALASSSPAVEIESMLIQC
                     ELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIAVEDSTIGIKSAKAANIYTA
                     ALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            786..1010
                     /locus_tag="ACG80L_002888"
     CDS             786..1010
                     /locus_tag="ACG80L_002888"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFS5025060.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            1112..1345
                     /gene="relB"
                     /locus_tag="ACG80L_002889"
     CDS             1112..1345
                     /gene="relB"
                     /locus_tag="ACG80L_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS5025061.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            1335..1604
                     /locus_tag="ACG80L_002890"
     CDS             1335..1604
                     /locus_tag="ACG80L_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5025062.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT          609 a          272 c          352 g          481 t
ORIGIN      
        1 tttattttga gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc
       61 agattgatga ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg
      121 acaaatcatt aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa
      181 ttgatttcgc taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc
      241 gggatatacc attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca
      301 ttcaatgtga acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa
      361 gcaaaccaca tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta
      421 ttgcagtaga ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg
      481 cggcactaaa acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc
      541 tacaagaact ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta
      601 tatatctgtg ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa
      661 aaggtgctgg taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt
      721 aagtttttta ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga
      781 atattttgca acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca
      841 atttaggagg ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt
      901 acgggggtga agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta
      961 aaacgatcag tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata
     1021 tgatgcaaat cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat
     1081 gttagtacaa atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat
     1141 tcccgacgat gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac
     1201 cgaactttat cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt
     1261 acggagtgca attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat
     1321 ggagaaactg ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa
     1381 aaaaaatgga cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa
     1441 acacgactga cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc
     1501 gctatcgaat tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc
     1561 agatattctc aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa
     1621 gtagagtttc gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa
     1681 ctacccgata ctatcggttg gtttctgctt tttt
//
LOCUS       DAZUMB010000245         1711 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000245 DAZUMB010000000
VERSION     DAZUMB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.251"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..285)
                     /locus_tag="ACG80L_002891"
                     /pseudo
     CDS             complement(61..285)
                     /locus_tag="ACG80L_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(839..925)
                     /locus_tag="ACG80L_002892"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ACG80L_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          599 a          252 c          227 g          633 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc t
//
LOCUS       DAZUMB010000246         1708 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000246 DAZUMB010000000
VERSION     DAZUMB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.252"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1508)
                     /locus_tag="ACG80L_002893"
     CDS             complement(27..1508)
                     /locus_tag="ACG80L_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5025063.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          529 a          232 c          297 g          650 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
     1621 ttatttaatt tttttgttac cttgtttatt ctattgttta tttcctaggg tattttgtca
     1681 agatattctt gtttttaagg cggaaaaa
//
LOCUS       DAZUMB010000247         1620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000247 DAZUMB010000000
VERSION     DAZUMB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.253"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1284)
                     /locus_tag="ACG80L_002894"
     CDS             complement(<1..1284)
                     /locus_tag="ACG80L_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5025064.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALT"
     gene            complement(1348..1620)
                     /locus_tag="ACG80L_002895"
                     /pseudo
     CDS             complement(1348..1620)
                     /locus_tag="ACG80L_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
BASE COUNT          495 a          315 c          222 g          588 t
ORIGIN      
        1 tgttaaagct gaattatttt ggatatcaac attcttttgt ctaaaatatc tatcatattt
       61 ctcaaaaagc tgctgcttat cagtgtcttt cttccacgtt tgataaagat actctataag
      121 ttcgttgata ttaaaaactt cgtctacttt ttccaaatca tccatttgaa tatattttaa
      181 ttcattctgt cttttcataa ccttcatgcc gttgaaattc acccattgtg gtaaatgata
      241 catctgaata taaagttctt ccaaatcatc taagaaataa aaggcattgg cacaggtcaa
      301 ttgaatgata tttttcaaat tgccaacatt ttcactaaag cgatagttta ataaaaaatc
      361 taataattgt tgagatatca gtattttctt tgctagtctt tcttcttctg atttaaaaaa
      421 tatcttgatt aactgttttc tttcataaag agaacgactg tctaatgttg gaagtgttag
      481 ctttacaggt attcttctca acaaagttga taatagttta tcctcaggtg gttctgatgt
      541 tgcaaatata atccagcaac ttgatgtgta ccagttttca ttatcaccca tccgatgaaa
      601 tttcctcgta tccataaact gaaaaagttt ctcctgacat tcattgctca atgcatgaat
      661 ttcatctaaa aataagactc cattattagc taattctgct aaaccctttc ttccatcttc
      721 ggctcctgta tatgcacctt ttacatgccc aaaaaaattg gacaagaaaa gttctggatt
      781 attggcatat tcactacaat taactgaaat gaaagatgcc ttctcttcta tataattatt
      841 ggccaacaaa tccttataca cataatttgc taaatagctt tttccagttc cagtctcgcc
      901 atgaagtaaa atgggtaagc catttggcgg atagtagatt gctgctttaa tttgagcaat
      961 tacatttcgc agacttcctt gacttccgat aaccctttct attccaaccc taaaagagct
     1021 atcataccat ttttccgctt cctctgaaga gctaaaaatt tgttgatttg gaactttctc
     1081 cggtaaagaa aagaagatta ctggacgagt attgattttt ccaaattctc catcttttac
     1141 gaattcattc aaatattgag agacaatatt acgactaaca tttaattttt cagatatttt
     1201 ctgtgctgta ttgttccccc atttattttt aatctcagag ttctctatga cataattgac
     1261 aatgcgttct tttaattctt ccatcacagc acctcctcga cactattcga attttgccga
     1321 cagtattttt ccataaatat gacttgctaa aaaggctttg tttagatcat tcaattggaa
     1381 cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga tactctggcg aaatcgcata
     1441 ccccaaaata tcaacgattg ttgcaatcag atcttctttt gattcttcat ctgaaagtaa
     1501 ttcacgatac aacatcgttt gtttgcttac tgtgtcatat tcctctaagg attcatctgc
     1561 taaaagaacc acttgttcca acaaacggtc ggataaatat ttatagaaaa tcagccccaa
//
LOCUS       DAZUMB010000248         1607 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000248 DAZUMB010000000
VERSION     DAZUMB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.254"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1403
                     /locus_tag="ACG80L_002896"
     CDS             75..1403
                     /locus_tag="ACG80L_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025065.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            1414..>1607
                     /locus_tag="ACG80L_002897"
     CDS             1414..>1607
                     /locus_tag="ACG80L_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025066.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRS"
BASE COUNT          551 a          263 c          304 g          489 t
ORIGIN      
        1 aagacataaa gttttagttt ttctttatca ttggttataa ttaaattata aaaagatttg
       61 aaagaggtga gaaaatgatt ggcgaagata aattattgat gactatccag ctcacagact
      121 taaatgaaga agaaaaaaaa caggaaataa gcgattgggc tattttgaca cgtctgttga
      181 tccagccaac atttatccgc tctttcacac aacaagcgga tccgtatgat cttcgaaagc
      241 ttcaagaaaa aataatgcta gcatcggtaa gagacgaaag ttggttagtt gtaggaaatg
      301 acgagaacga atgtagtttt cgcttggaag ataatcaatt attgataaaa aatgtattgt
      361 ctatctctgt tttcaaagaa aagcaatttt tgattcgaga cttcattcag aaaaaaatga
      421 ttgctcatgg ggtgttcgcc tatatgcgag cttatagcga gtttatctat cacaatacaa
      481 aacagatctc tcaacggcta ttgtttgaga aaaaggatga aatcgaacat ttacctaaaa
      541 tgaaacaaca aaacggggaa gtagtagtcg attgcaacca atttccagga tacgatcttt
      601 tttaccaagg tttatgtttc acttcgtgtt gggaaatgta ttatagtcgc tattatcatc
      661 aaatcatccc taaaccgatt tttctcgaca tccaacaagt agaaaaagtg aaggaattag
      721 aaaacgaagt catatgtatc cagctttatc gagacccctt taactggcaa aagccaaaca
      781 atcaaatgtt tcaatcctat tttagggacc agcttgggtt tgatcatctt gcttgggaca
      841 atggtgtcgg tttattgaaa ccgccattcg ttgaatacat ttacactgac catacgatcc
      901 aatctgtcca gtatcagaat gcacaaatgc aacctgttcc taaaaagaat gcttcatttt
      961 ttgtaacaaa aagttatgat atccaacaag gagattataa agaaaggcgt gttcgcggaa
     1021 cactaaatgc tcaggcgtat tttccttggg ttgacgagaa tcgatcacgc atgatgtgct
     1081 ataaagttat cgatccgact gttgctttgg ataatggaat cgaagcttat tgttattata
     1141 ttagggaata tctagaagta gaggtaacag atgaaaaata tcaagagtat cttttatcct
     1201 taagaatcta tgttccttca gaatccctgt cagaattacc gttaaaggaa atcaaacata
     1261 gactctctga tgttaccttc aaacgaataa agaaaaagcg gcgaagtatc caattcgatg
     1321 tcaagaaagg tgagcagcat ctgcgtgtgc aatttttaga ttaccgagaa ttagaacaat
     1381 tgattacttt acaaaaaata tgaggtgaag aaaatggata aacaactcta ttatcgaata
     1441 atggttccgg catcattatt atccgtctgt ctcttttttg ttcctaattt tcctattcga
     1501 tcagtcatcg gttttttggt catgtttggc gggatgggac tttattatta tcttcttcat
     1561 aaggaaagaa aacctgcttc tgtttatatg aaaaaaacaa gaagcga
//
LOCUS       DAZUMB010000249         1551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000249 DAZUMB010000000
VERSION     DAZUMB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.255"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG80L_002898"
     CDS             132..344
                     /locus_tag="ACG80L_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025067.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACG80L_002899"
     CDS             506..1024
                     /locus_tag="ACG80L_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS5025068.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACG80L_002900"
     CDS             1031..1543
                     /locus_tag="ACG80L_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025069.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DAZUMB010000250         1551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000250 DAZUMB010000000
VERSION     DAZUMB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.256"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..335
                     /locus_tag="ACG80L_002901"
     CDS             <1..335
                     /locus_tag="ACG80L_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025070.1"
                     /translation="RTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSM
                     MFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELR
                     KLVEYGII"
     gene            322..>1551
                     /locus_tag="ACG80L_002902"
     CDS             322..>1551
                     /locus_tag="ACG80L_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5025071.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKI"
BASE COUNT          495 a          205 c          244 g          607 t
ORIGIN      
        1 caaggacaaa tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct
       61 tgctacttga aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt
      121 tttcaccatc tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt
      181 taaatgaata tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt
      241 tctgtgaaca ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag
      301 aattaagaaa gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt
      361 aagtccagtt tctggtatac tatagggaat ttttttatta agggaatttc attcataact
      421 attcctattt ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact
      481 tcatttgtat caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat
      541 gcaagttttg actataaaga tgacataaat gaatttatgt catcggtact ttctctagct
      601 acactttcgt taattgtgtt cttattaata gggaacatat attttttgtt ttcaaatgct
      661 ttttttgata tgaaccaact tgtatttaat ttgatgttat tacaaagttt tgctagtttt
      721 gtaattagtt atatccagtc ttattttacg attaacttta accattttaa atatttgatt
      781 ttatctttta tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta
      841 tattttgatg ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata
      901 ggtttagtaa tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag
      961 tggagatacg ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt
     1021 ttagcacaat tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata
     1081 tattcctaca ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac
     1141 gcctgggttc catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa
     1201 gttagtaact attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg
     1261 attgatttag caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt
     1321 ccgataacct taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt
     1381 tatgagaaaa aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata
     1441 ctattaaaca tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta
     1501 tttacgtatt ttttactttt tatttttcat tataatatcg ctaaaaaaat t
//
LOCUS       DAZUMB010000251         1519 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000251 DAZUMB010000000
VERSION     DAZUMB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.257"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1036
                     /locus_tag="ACG80L_002903"
     CDS             68..1036
                     /locus_tag="ACG80L_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5025072.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1047..>1519
                     /locus_tag="ACG80L_002904"
     CDS             1047..>1519
                     /locus_tag="ACG80L_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5025073.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRA"
BASE COUNT          485 a          215 c          345 g          474 t
ORIGIN      
        1 acatttaatc agttactttt attttggata aaattaacat ttttaaaatt ttaaggagga
       61 aatgttgatg gatgtagcaa aaacggttaa cgcaattgta gaaaaaaatg atggagagat
      121 ttctggaatt tatttggtgg cttgtggcgg ttcgctggta gatatgtatg tctcagatta
      181 ttttcttaaa gccgaagcat cgaagtgtat tacaggctta tatacagcga acgaatttaa
      241 ccatgtaacc ccaaagcaat tagatgataa atctgttgtc attctatgtt ctcatggagg
      301 aaatacagca gaaacggtaa aagcaggaga agtcgctcaa gctagaggag ctgtgactgt
      361 tggtctgact cataataaaa ctgcggattt attaaaggtt tctgatcata gtttccttta
      421 cgaatgggga gatgatgcaa aaatagtcaa taatcctatg gccatcattc ttgatttaac
      481 tgtatctttg gtcaatgtta ccgaaacatt tgaacattac ggtaaattca gagaaggcat
      541 gaaggttatc gataccgtaa ttgaaaatgg cattaagcag gtgcaggatc gtattaaagt
      601 gtttgctgat aagtacaaag atgaaacaat gttttatatc ttaggtagtg gaccatcata
      661 tggacatgct tatggttttt caatttgctc tttgatggaa atgcaatggc ttgattcaac
      721 ttctgttcat tctggagaat tcttccatgg accttttgaa gtaacagaca aagatacgaa
      781 ttttattctc ttgatgagca ttggacgcac tcgtgcttta gatgaacggg caaaacgatt
      841 ccttgataaa tatgcagaaa gagtagaaat cgttgatgca aaggaactag gattggatat
      901 gatatcagat gaagtttctg aattcttcaa tcccatttta ttctacagta ttctttcaga
      961 atatcgttca gctttagcag atgtacgcaa tcatgattta gatgttcgtc gctatatggg
     1021 gaaagtggag tactaggagg attaaaatga aggtattagg cttaggtgat aacgtcgtcg
     1081 ataaatatga aaatctggga attatgtatc ctggaggaaa ttcattgaat tttgcagttt
     1141 ttgcaaaaaa actgggacat gaaagtagct ttatgggagt ttttggctca gatgaggagg
     1201 cagctcatgt tttatcagca ataaaagaaa taggactgga caacagtcac tctcgctttg
     1261 aaaaaggcga gaatggctgt gccagagtga agattgatga gggcgaccgg attttccttg
     1321 gcagcaattc gggaggtgtt actcgtgaat acccgattca gcttactgaa gaagacagag
     1381 aatatttaaa ccagtttgaa ttgattcata tgggattata tagccatgtc aatcatctat
     1441 tagaagagtt acaaaatgtt tctggaaaga tttcttatga tttttctgac gactttacgg
     1501 aagaagaggt tcagcgggc
//
LOCUS       DAZUMB010000252         1509 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000252 DAZUMB010000000
VERSION     DAZUMB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.258"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..868
                     /locus_tag="ACG80L_002905"
     CDS             101..868
                     /locus_tag="ACG80L_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5025074.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="ACG80L_002906"
     CDS             1060..1458
                     /locus_tag="ACG80L_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS5025075.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DAZUMB010000253         1492 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000253 DAZUMB010000000
VERSION     DAZUMB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.260"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG80L_002907"
     CDS             131..505
                     /locus_tag="ACG80L_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5025076.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG80L_002908"
     CDS             complement(619..1248)
                     /locus_tag="ACG80L_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5025077.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG80L_002909"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG80L_002909"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          507 a          226 c          261 g          498 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aa
//
LOCUS       DAZUMB010000254         1485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000254 DAZUMB010000000
VERSION     DAZUMB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.261"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1441
                     /locus_tag="ACG80L_002910"
     CDS             <1..1441
                     /locus_tag="ACG80L_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS5025078.1"
                     /translation="FGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANGGLGLP
                     RATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALPFGVST
                     LFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAPLIVGT
                     IGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFIRNLII
                     ALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEKPRVWA
                     YIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIFILTPV
                     FVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLGSFFIV
                     VIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGTESAYF
                     GIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          402 a          285 c          312 g          486 t
ORIGIN      
        1 cttcgggcag ccgaagggat tgtcaacttt gttcttcact gagatgtggg aacgatttag
       61 ttattacggt atgcgtgcat tacttatcta ttatatgtac gatacagttg ccaacggggg
      121 acttggatta ccacgagcaa cagcattagc tatcatgtcg atctacggat ctttgatcta
      181 catgtctagt atcgtcggcg gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt
      241 attcttcggt ggagttttca ttatgcttgg ccacatcgtc ttggctttac catttggtgt
      301 aagtacattg tttgtttcga tggcattgat tattatcgga accgcctttt tgaaaccaaa
      361 tgtttctggc atggtcggac atctttattc aaagactgac ttgcgaagag atgcaggatt
      421 ttcgattttt tacatgggtg tcaacttagg ggcattatta gcaccactaa tcgttggtac
      481 aatcggtcaa gaatataact atcatttagg gttctcttta gctgctattg ggatgttctt
      541 tggattatta caatacatca tccaaggacg caagacatta gacggagtgg gtacaacacc
      601 gccgaatcct ttgacaactg aagaacgtaa aggattcatc cgtaacttga tcatcgcact
      661 tgttgttatc gctgcagtat tcggcggggc acaactaact ggtcatctga caattgactt
      721 ttttgtcaat acaatcagtg tattggggat tatcttaccg ttgtattact ttatcaaaat
      781 gctgacttca aaagatgtaa ctgctgaaga aaaaccaaga gtatgggctt atatcccatt
      841 attccttgca gcaatcattt tctggtcatt acaagaacaa ggttcatcta tcttagcttt
      901 atttgctagt gaacgtacac aatctacgct atttggtgta acgatccaag caagctggta
      961 tcaatcattg aatccaattt tcatctttat tctgacacca gtattcgtaa cgatgtggac
     1021 gaaacttggt gaacgtcaac catctacagt agtgaaattc tctcttggac ttttatttgc
     1081 aggattatcg tacttgctat tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt
     1141 aagtccattg tggttgttgg gaagtttctt catcgtggtt attgctgaat tgtgtctttc
     1201 accagttggt ttgtctgtga caacaaaatt agcaccacga gcatttgaat cacaaacaat
     1261 cgctatttgg ttcttagctg atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt
     1321 ctacacaccg ggaacagaat ctgcttactt cggtattatc ggtgcagtag caatcgtcgg
     1381 tggtatcttg ctatttactg tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta
     1441 atcaatgaac ataaaaacag gaattgctta tttgcagcag ttcct
//
LOCUS       DAZUMB010000255         1470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000255 DAZUMB010000000
VERSION     DAZUMB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.262"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACG80L_002911"
     CDS             105..1406
                     /locus_tag="ACG80L_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5025079.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          483 a          296 c          268 g          423 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat cagtcctatt tgacaaagag
//
LOCUS       DAZUMB010000256         1451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000256 DAZUMB010000000
VERSION     DAZUMB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.263"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACG80L_002912"
     CDS             complement(<1..625)
                     /locus_tag="ACG80L_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS5025080.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACG80L_002913"
     CDS             complement(784..1050)
                     /locus_tag="ACG80L_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5025081.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACG80L_002914"
     CDS             complement(1040..1396)
                     /locus_tag="ACG80L_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5025082.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DAZUMB010000257         1437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000257 DAZUMB010000000
VERSION     DAZUMB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.264"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG80L_002915"
     CDS             complement(138..428)
                     /locus_tag="ACG80L_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025083.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG80L_002916"
     CDS             complement(546..806)
                     /locus_tag="ACG80L_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025084.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG80L_002917"
     CDS             complement(943..1356)
                     /locus_tag="ACG80L_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS5025085.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          530 a          237 c          211 g          459 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataa
//
LOCUS       DAZUMB010000258         1417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000258 DAZUMB010000000
VERSION     DAZUMB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.265"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG80L_002918"
     CDS             complement(150..1337)
                     /locus_tag="ACG80L_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS5025086.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZUMB010000259         1395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000259 DAZUMB010000000
VERSION     DAZUMB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.267"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..715
                     /locus_tag="ACG80L_002919"
     CDS             137..715
                     /locus_tag="ACG80L_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5025087.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            715..>1395
                     /locus_tag="ACG80L_002920"
     CDS             715..>1395
                     /locus_tag="ACG80L_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5025088.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLS"
BASE COUNT          452 a          270 c          235 g          438 t
ORIGIN      
        1 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
       61 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
      121 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
      181 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
      241 ttccagtccg atgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
      301 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
      361 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
      421 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
      481 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
      541 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
      601 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
      661 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
      721 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
      781 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
      841 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
      901 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
      961 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     1021 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     1081 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     1141 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     1201 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     1261 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     1321 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     1381 ttacaacttt tatcc
//
LOCUS       DAZUMB010000260         1382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000260 DAZUMB010000000
VERSION     DAZUMB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.269"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1347
                     /locus_tag="ACG80L_002921"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ACG80L_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
BASE COUNT          482 a          190 c          255 g          455 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 ga
//
LOCUS       DAZUMB010000261         1355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000261 DAZUMB010000000
VERSION     DAZUMB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.270"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG80L_002922"
     rRNA            <1..1355
                     /locus_tag="ACG80L_002922"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZUMB010000262         1348 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000262 DAZUMB010000000
VERSION     DAZUMB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.272"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          501 a          157 c          281 g          409 t
ORIGIN      
        1 ttaatttgac aattaagaaa gtattaactt gttttgtgaa aaagagaatt tgaaaaacga
       61 ttggcgaaca aatgctcggt aggtatggta taaatacaga gctaattggt gtgatgaaat
      121 cagatattcg cgtgagattc agtaaagtag gagttcgaaa acagctaggt gacaaacagt
      181 attgctttgg tacttcggaa tggtttatca aattcttatc aacagttcac ttctctttat
      241 gtttgagtga tgattcgtta ggttattgct tggaaagacc ggatgagagt agaagagttt
      301 aattttataa agtgaaaaga ggtaatgtac gatatacgta gggctgaacc aatagaggat
      361 aagcaaacga tgttacaggc gcaaaattca tagcttactg ttttggagat aacagaagag
      421 gaaataatgc agaagtaagg cgaaaaaagg acggaagcct aacagcatat gaaataaaga
      481 aaaatattat aactactgat aaatcggtat tgagcaaagt actaatagga gtcaaaaaca
      541 ttcataagct atgagtgttt cttggctact tttttatttt tatgtatcta caaaagtttc
      601 caggggcaga acctgtgatt attaatggaa gaacctcaag tattgtctta aatcattgtt
      661 atgaaaatgg attaaaagag ttcatcaagg tgtgattaac aagttacttt tcattacatc
      721 gaatttttca gaagttgctt atattggtga tgatattaat gatttatcat gtatgacatc
      781 tgtgaagaag cgggaggaat agtagaatgt ccaaaaaatg ccacaagaaa tgagattgaa
      841 ttatctgatt ttattgtaac tcacagtggt ggtgacgaat ctgtgataga gtttattgag
      901 tggatattgg aatcgatata gtgtagtagt acagaaaaat tatcatacaa ttaatactag
      961 gcataagaat attggcatat tctagagtta aaagtaacta ttcgaaaagg ataaggttag
     1021 aattttttgt tttgccagtg gaagaatata aaaagggcat tggaaattaa tatattagaa
     1081 tgctaagcgg agaaaaaaat atatgaaaag tacgtccttg tgatgggatg gaaaaataca
     1141 cttatttcaa acaacgataa tataattatg tgaaccgaag tatactcgct tctaattcaa
     1201 tcctcaaatc ccgacagtcg cgacttcaaa aaagcgtata gttttaatgt atccccatat
     1261 gatccgaaaa atggtaattt acaattaaca gtttgatgat gttgaagatg tggaaatgct
     1321 gaaattacca tgatgagaaa cacattaa
//
LOCUS       DAZUMB010000263         1310 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000263 DAZUMB010000000
VERSION     DAZUMB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.273"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1310
                     /locus_tag="ACG80L_002923"
     CDS             <1..>1310
                     /locus_tag="ACG80L_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5025089.1"
                     /translation="LKYYDFSAQDIAMKFCIKRNVASHYLNQLFSDGKLLKNDSVRPV
                     MFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLPLIIKGNSGVGKSFLAS
                     LIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEKDTAGMLSS
                     ANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILATTEDKNTS
                     LLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIFCSIELFTDLLSK
                     TFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWIDPEISIDLKKMFLKSL
                     SEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESDEFIALRNYVVNKISPI
                     IDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMK"
BASE COUNT          467 a          171 c          233 g          439 t
ORIGIN      
        1 tttaaagtat tacgattttt ctgctcaaga tattgcaatg aaattttgta ttaaaagaaa
       61 tgtcgcttcc cactatttga atcagttatt ttcagatgga aaattactaa agaatgattc
      121 tgttagacca gtaatgttca agtataatca acaaaagcca aaagactgct tttctaagtt
      181 tattggagca gacatatctt tgaaatccac tatagataaa tgcaaagcta ctgtaatgta
      241 tccccctaat ggcctcccat taataataaa aggaaacagt ggcgttggaa aaagtttttt
      301 agcaagtttg atctatcagt atgctcttga tagaaaagta attcataatg acgcaaaatt
      361 tgttgttgtg aattgtgcag attatgcaaa taatccagag ctattgtcag cagttttatt
      421 tggatataaa aaaggagcct ttactggggc tgaaaaagat actgcaggga tgctatcttc
      481 tgctaatgga ggataccttt ttttagatga ggttcacaat ctaagtgcag aaaaccaaga
      541 gaaattgttt cttcttatgg attctcaaaa gtatcgtaaa cttggtgaaa gtgtgcaatg
      601 ggagtatgca aatgttcgcc ttatcttagc cacaacagaa gataaaaata cctctttgtt
      661 agcaactttt agaagacgca tcccagctga aatcactctg ccagattaca ataatagaag
      721 tacatctgaa aaaattcaat tattatttga atttcttaaa gatgaagcaa ttacgattga
      781 ttcaaaaata ttttgttcga ttgaactttt tactgatctt ttatcgaaaa catttgaagg
      841 aaatgttgga gaattgagga atgaaatcaa gcttctttgt gcagaaggtt atttaggaaa
      901 taagagaaag tcttcaattt atttagggga caaactagac agcggttttt ggatagatcc
      961 agaaattagt atagatttaa aaaaaatgtt tcttaaatca ctttctgaaa ttgatttgat
     1021 tgaacttttt caaaatcaca tttcaattga agaaagcatg agtcaattaa gagatagaat
     1081 acttaaagag tgtgcaggtt cttctgagta taattattta gaaagtgacg aatttatagc
     1141 attaagaaac tatgttgtta ataaaatatc accaattatt gattcgacag gtctatgttt
     1201 attagatgaa ttcttggcgg atatcagtct atttataatg tttattgatg taattgaaga
     1261 aacatcaaga aattttttgc tgtctaagtt cagaaaattt cgtatgaaaa
//
LOCUS       DAZUMB010000264         1302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000264 DAZUMB010000000
VERSION     DAZUMB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.275"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..>1302
                     /locus_tag="ACG80L_002924"
     CDS             52..>1302
                     /locus_tag="ACG80L_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5025090.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAZUMB010000265         1299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000265 DAZUMB010000000
VERSION     DAZUMB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.276"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..396
                     /locus_tag="ACG80L_002925"
     CDS             <1..396
                     /locus_tag="ACG80L_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5025091.1"
                     /translation="FAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKK
                     KKVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLT
                     TEQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            641..>1299
                     /locus_tag="ACG80L_002926"
     CDS             641..>1299
                     /locus_tag="ACG80L_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025092.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     V"
BASE COUNT          523 a          224 c          233 g          319 t
ORIGIN      
        1 tttgcgattg atcaaacaat gaaaaacaaa aaaactcgaa cgtataacca attttgtgat
       61 tctcttaaaa cattcggcat tcattgcttg aatcgtggga aaaattttac ttacgaattg
      121 atccacaaaa agaaaaaagt acgatccaac aaactaggaa aggattacga aaaggagacg
      181 attttacatg agctggacag acgagaacga gcctacaatc aacaacgaac caacgagcta
      241 cgaagagctc gagctagcat tcgcgaaact gcaaaagaag tacgaagaga acaacgaact
      301 cttacgactg aacaaccgga aattagaccg actcgaagaa accatgacca acacgttgaa
      361 agaggagcag actaccaaca agaattatcg ttctaaaacg ttacaagaca tagaaaatga
      421 aataaagaaa caacgtgctt ttcttgcacc ttataccaaa gaaagaatac aaactacaat
      481 tgattcacaa gtcaaaaaat tccaccatgc gctattaaac cagcgaaata aagaacagaa
      541 aaaagtaatt atgcagcgat caatggatat tcttgccccc ttatgttgct taatcatgac
      601 actgttagcc ttttggctgg tatggggaga ataaatagaa atggcattct atttacccta
      661 tctactcatt tttgccagta ttttaggaat tatttggctt agctataagg cttatcaaac
      721 aagatattca ttaaaaggct ctaaaattcg atttaagagg tttttgttgc caggggttat
      781 atttacatta tcaatcgttt taagtggttt attcggcgtt ttagagggaa ataaacgtat
      841 ttcaagaagt attttattag gggatatcac tcaaaagtat gaagcagcat gtgataaaaa
      901 gaaaaaggag caagtgttaa cacaaaaaat ggaagagttt accacttgtt atgaggaaat
      961 gaacgatatt tttgtgaatc aagaaaaacg tttgacggat aaaaacatgg aaaaactcac
     1021 tcgtctttat caaaacttgc cagaaaaaca gcaaaaagag gttcaagaca actatgagcg
     1081 aaccaaaaaa gacgtgcaat attttaaaga tacgcagaca gaagaagctt gttataattt
     1141 attcagtgat acaatccctt tttcaactag tgaacaggaa cgaaaagaac ggcaacaatc
     1201 agttacctac gaacgttata aagcactgct tcagcaagca acgaacatac aaaatccaac
     1261 gaaaaaagaa acggcattaa attatctaaa aagtgtcaa
//
LOCUS       DAZUMB010000266         1294 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000266 DAZUMB010000000
VERSION     DAZUMB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.277"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG80L_002927"
     CDS             <1..1244
                     /locus_tag="ACG80L_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS5025093.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZUMB010000267         1280 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000267 DAZUMB010000000
VERSION     DAZUMB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.278"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            308..1219
                     /locus_tag="ACG80L_002928"
     CDS             308..1219
                     /locus_tag="ACG80L_002928"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5025094.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          502 a          194 c          251 g          333 t
ORIGIN      
        1 agtgtctgat ttgaatatcc cagcagcgga agaagaaaaa caaaatcgat aacatttgtt
       61 ctaagaatta gttacgttaa atacggaacg caagatagac ttgaaaaacg gtaacaaaag
      121 aagtagtata ttaaggtgtg atgtgctact tgacatttat tctgctgagc gaactttctg
      181 gattgtgtag ccttatatct attcaggacg tatcgagcag attagtaaag gtgttcttga
      241 tgattttcat cttgaacacc tttgttttta gcaattcgca aaaaacgaaa agtaggagaa
      301 ttgataaatg aaaaaaagat ttttagcact tgccatcgta ctaggcactg gcttattgtc
      361 aggctgtacg aatgccggtg aaaaaacagc cgttagttat aaaggcggta caatcagcga
      421 acaagaagta atggatagct tgaaaaaaat gcaaggagca gattccgctg tgcaacagtt
      481 gatcgtttat caagtatttg aagataaata tggagatgac gtatcaacca aagaaatcga
      541 ttctcaatat gatcaaacaa agaaacaatt gggtgattca tttgattcac aattgaaatc
      601 tgcaggttac acagaacaaa cattcaaaga cagtatcaaa caaagtctag cctttcaaga
      661 aggtctaaag aaacatatca aattgaccga tgaagattta aaaacggctt gggaatcttt
      721 ccatccagaa gtggaagctc aaattatcca agtagcaagt gaagatgatg caaaagatgt
      781 gaagaaagct gctgataagg gagacgattt ctctaaatta gccaaagaca agtcaactga
      841 tactacgaca aaagaagatg gcggaaaagt gaaatttgat tccactacta caacggttcc
      901 ggcagaagtc aaagaagcag cctttaagtt gaaagatggt caagtttctg atgtgatcac
      961 ttctaccaat gcttcaactt atactacgga atattatgtg gttaaaatgg tgaaaaacca
     1021 aaacaaaggc aacgacatgg ataaatacaa aaaagaattg aaagaaattg caacagacac
     1081 aaaattaagt gacagtacct tccagaataa agtaattggc gaagtattga aagacgctaa
     1141 tgtaaaaatc aaagacaaag actttgaaaa tgttttgagt acattcactt cagattctag
     1201 tacagcaagt agtaaataag cggtataaat aaaaaatatg aactatttaa atcaataaaa
     1261 ctgttcatct aaatcttttt
//
LOCUS       DAZUMB010000268         1276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000268 DAZUMB010000000
VERSION     DAZUMB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.279"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..427)
                     /locus_tag="ACG80L_002929"
                     /pseudo
     CDS             complement(107..427)
                     /locus_tag="ACG80L_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(543..>1276)
                     /locus_tag="ACG80L_002930"
     CDS             complement(543..>1276)
                     /locus_tag="ACG80L_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025095.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT          403 a          217 c          171 g          485 t
ORIGIN      
        1 aagcaaaaaa ataaagaaac ttattcttga taacaagcta cgtgacgtat atccctaaca
       61 caaaaatagc catatccttt ggtttggata cggctatttg atattactca ttcaattatc
      121 aaatcacagt aggtgtgaat ctcatcggga tcggcaatat ctacttctga ttaggatcaa
      181 aggtgatttt ctcaataaag gtgtgataca aagctttaac ttgcttcttc tcactctgga
      241 taagaaagca atccaaacta gtcaatatct gttggacatc tttgacttga atcggttccc
      301 cttgttgttg aaagatccct aagatttcat tctctcgaat ttgtaactgt cttcgttttt
      361 cagtcaattc atttaaacga tcttttaaca tcggaaacaa ttcgggacta tcttccaaag
      421 cagcttcctg tttttgctct aatagttaga ttttgcgtac cattttcttc ttttattttc
      481 cttcgattgc tgattttaat atagtctaaa ggaaatttat atttattgta ttctttataa
      541 cttcaagaga acttcgtttg agtattactg tattttttta attccataag aaaattagtt
      601 attacctgcc acctaatcag caaatctgaa cctgagtcaa tataattaat attattttcc
      661 atctccaaca ttgtaagtac tgttggtgtt aatataggga ctttgtacaa ttgtattttt
      721 ttgattttat catctgcaca aaggagattt tttaaatcta atccttcttt tatttcaaaa
      781 gttgaatttt ttaaatctat aagagtaaaa ccaattcctt catactgaat ttctgaaaat
      841 cgaatttttc tcccagttga tagatcaagg ggcagaaaac cttttattac attgtgagtg
      901 tcaccatcat ttacaatgtc attaggaatt ttgattttag aattctgtgg agcacctatt
      961 ggaagataat aattcattat aattttgtag tcttcatgag gaaataaagt acttttagga
     1021 ttgctaagat attctcgtac cgaatctaag aatggcttat ccattgaatc ttcatcccca
     1081 tttaaactaa aaaacattcc tacaattgct tttgcaactc taagtgaatt cattttcttt
     1141 attgaatgtg ttaaatcaga cttaccattc tttaaacttg tattgttata aaggaaattt
     1201 ttattgtctt ctgcaaatct aatatattcc tcaacatact cactaccaaa aaaactatta
     1261 catttactac ataacg
//
LOCUS       DAZUMB010000269         1272 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000269 DAZUMB010000000
VERSION     DAZUMB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.280"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1272
                     /locus_tag="ACG80L_002931"
     CDS             <1..>1272
                     /locus_tag="ACG80L_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS5025096.1"
                     /translation="SIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRACRS
                     CGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYF
                     VQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEF
                     LVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAE
                     KLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFF
                     HELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIE
                     AKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          426 a          249 c          244 g          353 t
ORIGIN      
        1 tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga ggtctcttac
       61 gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt
      121 gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa
      181 aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttatg
      241 cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac ggctcaaagt
      301 tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat tgcttcttta
      361 ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc tttgaccacc
      421 gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa gaagatcctc
      481 cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga taagatgagc
      541 tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac gcgtaaatta
      601 cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga attcttggtg
      661 acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa tgcgaaagtc
      721 gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga gttgcgtatc
      781 cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa actgaaaaag
      841 aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata caaaacatgg
      901 aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat tgatcgattg
      961 cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt agttgaagcc
     1021 tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta
     1081 gatgacagct ttcgggaaaa gcttcaaaac cttctgacct atgaagaagg catcaccaac
     1141 gcaatgatct atccttattc caatggaaaa atagaagcga agaataccca cataaagaca
     1201 atgaaacgag tatcctacgg atttaaatca tttgagaaca tgagaattag aatctttttg
     1261 atcaatcaat ta
//
LOCUS       DAZUMB010000270         1265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000270 DAZUMB010000000
VERSION     DAZUMB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.281"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="ACG80L_002932"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="ACG80L_002932"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="ACG80L_002933"
     tRNA            203..276
                     /locus_tag="ACG80L_002933"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="ACG80L_002934"
     tRNA            316..405
                     /locus_tag="ACG80L_002934"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="ACG80L_002935"
     tRNA            420..491
                     /locus_tag="ACG80L_002935"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="ACG80L_002936"
     tRNA            507..579
                     /locus_tag="ACG80L_002936"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="ACG80L_002937"
     tRNA            589..661
                     /locus_tag="ACG80L_002937"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="ACG80L_002938"
     tRNA            697..772
                     /locus_tag="ACG80L_002938"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="ACG80L_002939"
     tRNA            774..854
                     /locus_tag="ACG80L_002939"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="ACG80L_002940"
     tRNA            861..934
                     /locus_tag="ACG80L_002940"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="ACG80L_002941"
     tRNA            937..1009
                     /locus_tag="ACG80L_002941"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="ACG80L_002942"
     tRNA            1021..1092
                     /locus_tag="ACG80L_002942"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="ACG80L_002943"
     tRNA            1138..1208
                     /locus_tag="ACG80L_002943"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
BASE COUNT          306 a          282 c          331 g          346 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaa
//
LOCUS       DAZUMB010000271         1256 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000271 DAZUMB010000000
VERSION     DAZUMB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.282"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG80L_002944"
     CDS             <1..969
                     /locus_tag="ACG80L_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS5025097.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACG80L_002945"
     tRNA            1159..1232
                     /locus_tag="ACG80L_002945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          415 a          229 c          256 g          356 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttc
//
LOCUS       DAZUMB010000272         1179 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000272 DAZUMB010000000
VERSION     DAZUMB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.285"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..1174
                     /locus_tag="ACG80L_002946"
     CDS             146..1174
                     /locus_tag="ACG80L_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025098.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
BASE COUNT          445 a          175 c          214 g          345 t
ORIGIN      
        1 aaaaaattat ataagtttgc gtacgattca aagaaaatta agagaactta atactatttt
       61 gaaaaactat catttatctt gcagcttgaa aaaaggtaat ccgatagaag gagaagaata
      121 ccgtatccga tacttttttc acttgatgta ttggcagtca ttcgacgaaa atcattctga
      181 tatattatca agtataaaaa ataaacgtca actattagaa aaatttgcga aacatgcgcc
      241 atatacgcgt agtatagata gaaagaaatg gatcagccta ctaagaattt cgttgcaacg
      301 aatgaaacaa ggccactcga ttacgtatgt cccagaagag atgaacaaat ttgtgcatcc
      361 attgataaca aaagaagagt ttacgcatta catactagag cctttttttg aagcaaactt
      421 ctggtcgatg aatcacatca ctccaacaga gcaagcttac ctttatttta tgtttggtgt
      481 aatgaacacc tatttggaag aagatttgtt ggatactcca gataaagtac aagaaagttg
      541 gggctggatt gaatcgtcta ttgtagaaaa ttttgaaaaa aacttcaaga tttgttttac
      601 aacttcagaa aaaaattatc tatatgtcaa tttggcaatg atccatttgt attcaagggt
      661 ttttgggaca aaaaagaagg tggatgcttt tggaaaatca gcagaagaca gagattttca
      721 gcatatattt ccagtgatgt atccaatcat gcaacagttt tttagtgggg tagctcgcag
      781 aattccagag cttaatactt attatgaaaa gaacagacgg ttgatttttc aatactgtat
      841 gttagtcagg gatattttta taaaacacga acaacctgtc atattctata ttcaatcaaa
      901 gtatggtaaa acacaggaat tatgggcaaa gaaaaggata ttatctatga ctgaacgccc
      961 aataaaatat agtgcatccg ctgatcagtt acctgatatt gtcattagcg attatcctat
     1021 cgacaaaaag gcatatgatc aaacgaatac acagatattt tactggaatg gacaacctac
     1081 aaaaaatgat tgggcgcgtc tgaaagctga aatcataaaa atcaggaatg agaaaaatag
     1141 atccatattg gaaaaattgg atttcgttaa ttaagaaaa
//
LOCUS       DAZUMB010000273         1174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000273 DAZUMB010000000
VERSION     DAZUMB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.286"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..274)
                     /locus_tag="ACG80L_002947"
     CDS             complement(<1..274)
                     /locus_tag="ACG80L_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5025099.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDP"
     gene            complement(285..953)
                     /locus_tag="ACG80L_002948"
     CDS             complement(285..953)
                     /locus_tag="ACG80L_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5025100.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1083..>1174)
                     /locus_tag="ACG80L_002949"
     CDS             complement(1083..>1174)
                     /locus_tag="ACG80L_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5025101.1"
                     /translation="KHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          440 a          201 c          156 g          377 t
ORIGIN      
        1 taggatcttg acttacgtag ccaatatttt ttgatatact ttcttgtgtt aaggattgta
       61 gcgagttatt ttcaaataaa atttccccaa ccaattttcc gtcataaagt cttggaataa
      121 gtccattcat taactttaat agtgtggatt tcccactccc agaagaacca gtaatcaatg
      181 taagactgcc tttttttatt gaaaaattaa ggtcaacaag cgcttgttcc ttaacatcct
      241 tatatgaaaa agaaacattg ttaaattcaa acaattcttt cacctcaaat caaaatactt
      301 aagaaaaaaa tagtaacgga aaacaggatg atatagtcta ataagctcat gtttgtttta
      361 tagtaagaac ttcttttcac aggagattct atcccctttg ttattgcatt agcggtcatt
      421 tcttcagcaa tcatagttgc acgtataaga acgggaacta acagaaattc caaatattgc
      481 agaggctgaa aaaaagacaa gcgaatgttt ctcattttta aagattcttt aatcattctt
      541 atttcagaac gataagtcgg aaagaatctg atcactactg taatacttaa cattattttt
      601 ttcggtaaat gtattttttc taaagaggat aataattcat tgctagtata gacactaacc
      661 aacaaccatg agatcattac gaccggcaac attcgaaata caatatataa ggatgtcgca
      721 aaaaatatag taaaaaactc tatcttgata gcatcaataa ttttcattac taaaaacaga
      781 actgaataaa tcaagatgaa ataaatacat gtctttattt tacgaaacat caatactaaa
      841 actgtagcaa taatgaaaca ccccacttga aatatatttc cctttcctaa aaaaataatg
      901 accggaaaaa aaatatttaa agtaatcaag gttaacggat tgatttttga cactctttca
      961 cctccaaaaa ataagatcgc actgttatct taagctgata aaaatgcacc gttattcaga
     1021 taacagtgcg atcttatcaa aaagcataac tgagaacaat tctcaatgca agcttttttt
     1081 aattaactca gtaattcaat ccacccagga acagaaaatt gatacaatct tctggttaat
     1141 agcatagtct tttcttttga atagctatgc ttca
//
LOCUS       DAZUMB010000274         1168 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000274 DAZUMB010000000
VERSION     DAZUMB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.287"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            335..574
                     /locus_tag="ACG80L_002950"
     CDS             335..574
                     /locus_tag="ACG80L_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025102.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            631..759
                     /locus_tag="ACG80L_002951"
     CDS             631..759
                     /locus_tag="ACG80L_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025103.1"
                     /translation="MQPLQMLYDNKPELNDPDISFAFFEMIGSDDLFKTTSLSIRK"
     gene            complement(936..>1168)
                     /locus_tag="ACG80L_002952"
     CDS             complement(936..>1168)
                     /locus_tag="ACG80L_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025104.1"
                     /translation="KYICQPIGLKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKC
                     GKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          386 a          212 c          166 g          404 t
ORIGIN      
        1 gaagtaataa tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc
       61 ctagcaacta tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc
      121 acttataaat tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa
      181 ccaatgtatc tggtttgaac tgggatatca ccctctccat atatgtatta ctaagactaa
      241 tagatactat aaaagacttg atagttttgc attgattttg cctaatactg agcataggtg
      301 tatgctaaac gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt
      361 atcaaattta ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga
      421 tacgcaaatt tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt
      481 agaaacttcc gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc
      541 acgtctcctt tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat
      601 gatgatagtt tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac
      661 aagcctgagc taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat
      721 gatcttttta aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt
      781 ttttatcgag tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt
      841 gaataaatca aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg
      901 gtcttctcgc ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat
      961 ttgttgctat agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt
     1021 gccactactt ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct
     1081 acgcagatca caacacaatt ttctaattta ctgattactt ctccaaccac tttttgtttc
     1141 aagccaatag gttggcaaat atattttt
//
LOCUS       DAZUMB010000275         1157 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000275 DAZUMB010000000
VERSION     DAZUMB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.288"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1157
                     /locus_tag="ACG80L_002953"
     CDS             <1..>1157
                     /locus_tag="ACG80L_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFS5025105.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGF"
BASE COUNT          379 a          229 c          226 g          323 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggattt
//
LOCUS       DAZUMB010000276         1150 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000276 DAZUMB010000000
VERSION     DAZUMB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.289"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..194)
                     /locus_tag="ACG80L_002954"
     CDS             complement(<1..194)
                     /locus_tag="ACG80L_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025106.1"
                     /translation="MKNFDTYYVIVFSISLLLILLTLMQYKEYPEYFILTFVYLVSSY
                     TLLYKFRSSSRVSKVCFLIS"
     gene            complement(208..438)
                     /locus_tag="ACG80L_002955"
     CDS             complement(208..438)
                     /locus_tag="ACG80L_002955"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS5025107.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(532..870)
                     /locus_tag="ACG80L_002956"
     CDS             complement(532..870)
                     /locus_tag="ACG80L_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025108.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          388 a          221 c          126 g          415 t
ORIGIN      
        1 aagctaatta agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt
       61 gtgtagctac ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac
      121 tgcattaaag ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg
      181 tcaaaatttt tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc
      241 ttctacttca gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga
      301 tccttctctg taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc
      361 tttccactca ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt
      421 tcccttttct accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta
      481 catctagtaa attttcatca atttctatat cataaagtat ttcactagtg tctacatcca
      541 caatatatac tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc
      601 gtcactttca tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag
      661 ctctaagcct actgtatttt ttactattga gtcattattt accaccataa cttcgtactg
      721 tatataccat cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt
      781 ctccggaaca atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc
      841 atgttcctca cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa
      901 aataaacact acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc
      961 caatcacaaa ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac
     1021 tacaactatt catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga
     1081 tacttgtaat agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa
     1141 ttccaataca
//
LOCUS       DAZUMB010000277         1143 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000277 DAZUMB010000000
VERSION     DAZUMB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.290"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACG80L_002957"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACG80L_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS5025109.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAZUMB010000278         1132 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000278 DAZUMB010000000
VERSION     DAZUMB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.291"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..>1132
                     /locus_tag="ACG80L_002958"
     CDS             25..>1132
                     /locus_tag="ACG80L_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS5025110.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAY"
BASE COUNT          387 a          169 c          255 g          321 t
ORIGIN      
        1 gatttcaagg agtagatttt atttatgaag caaatattgc ggtcaaaaat gatttttgat
       61 ggagaacagc cacctttcaa aggttatatt gtaatcaatg aagggatcat tgaaaaggtg
      121 gaaaacaact ggaattacag tgatctatta aatgaaaaca cggaattaat tgcttatgat
      181 gatcaattcg ttatgccagc aattcatgat aatcatgtat ttttcagtgg ttatttggct
      241 atgaatgctg gcatagattt aagcgataca tcttcagcta acgaggctgt agagcgtatt
      301 aaagctgtaa tgaaccataa aaaagcagat gaaccaattt atgctcatgg ctgggatgct
      361 gccctgtgga aaactgaccc atcagaagag ctattgaatg aaataaacca ctttggtcca
      421 atcacagcga tcgacaaaaa tcgcagccat tgttggatga atgctattgc taagcagact
      481 tatggtttta cagaaaaaga aaccagtgct gaaagtcgag tattattaat taaggaaatg
      541 ctagcaaaca aaaagctttt agcagaggtt tacgctgaat ttgaagcatt actgctttca
      601 aaaggtgtta tatcaatgaa agaaatcgtt tttgatgacg cagaatttct ttctaatata
      661 cctcagagga aaatacttag taacctatat gtgcaggcag tccaagaacc tctacagcat
      721 gaacttctat atgagtataa ggaaaaagtt ttccctgaaa aggtccgctt tggtggagtg
      781 aagatcatgg tagatggtgt tgttgcggat agaagtggag atatttacgg tgtttatcag
      841 tcaggagtag cttcacccga gattgactat gaggcaatta aagcagaagt tgctgcattc
      901 aatgaagtag agattccttg ctgtttgaca acggaaggag ataaagcggg ttataaagct
      961 gcgtcaattc ttgtcgaatt tggaaagcgg ttacctgaag gcgtttttaa ttcgattagt
     1021 gatttggaaa tggttactca aaagacagca gaaaaaatga acgaaggaca agtagtagca
     1081 gaggtatatc cacaaattct cggcttgaat caaacgttag aagaggctta ca
//
LOCUS       DAZUMB010000279         1116 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000279 DAZUMB010000000
VERSION     DAZUMB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.292"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="ACG80L_002959"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="ACG80L_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5025111.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DAZUMB010000280         1111 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000280 DAZUMB010000000
VERSION     DAZUMB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.293"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..547
                     /locus_tag="ACG80L_002960"
     CDS             <1..547
                     /locus_tag="ACG80L_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS5025112.1"
                     /translation="DVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNR
                     EIVPTVSQYRKLVSDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGG
                     LMASPYLPIQVGNIKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHI
                     KWLNEDIEIDILDEYKHDILI"
     gene            603..821
                     /locus_tag="ACG80L_002961"
     CDS             603..821
                     /locus_tag="ACG80L_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025113.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            859..>1111
                     /locus_tag="ACG80L_002962"
     CDS             859..>1111
                     /locus_tag="ACG80L_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025114.1"
                     /translation="MIKLRRNFKKIMKYNNLKPKLLNENIEYIREENYGYLTIVPKNG
                     TESKDNVFNPTVRYILANCTGKNSVKKIANGLKKKFPSLE"
BASE COUNT          417 a          138 c          212 g          344 t
ORIGIN      
        1 ggatgttgct ttttgtccta ctagttttaa ttacaatcaa ttacgagaag tatatcaact
       61 agtaaatact cttaaagtga agtctttaag aattcaacca tttatgctta gtggtagggg
      121 aatgcaaaat agggaaatag tgccaactgt ttctcaatat agaaaactag tttctgatat
      181 taactatatt aaaagaaatg aagattatac caagatagat tggggagatc caattgatca
      241 tatcatccga cacagaactt tattgaaaga tttttcgaat tttgtttcta ttaggtcaga
      301 cggaggatta atggcatcac cttacttgcc aattcaagtt ggaaatatta aatgtcacag
      361 tctttctaaa tattggcgag caggacttta taaagttttt tctttagatt tagttcaaga
      421 tatagctact caagtttctt caatacaaga tatgagtaaa gaattaaaaa aaggacatat
      481 taaatggctt aatgaagata tagaaattga tattctcgat gaatacaaac atgatatttt
      541 aatttaggtt tatcattgga aagtttagat aaacaaagta cttttagaaa ggagggaaaa
      601 aaatgatttc tgaacctcaa atgaataata tgtatgaaag cccaactata atgggagctg
      661 gtgaagctgc gccaagagct tgttcagcaa ttatggtgtg tgtgttatta gcatctgcag
      721 gagtaggtat ccaagcagga gcggtgtata cttatgctgt agttgctaca gtagctgtag
      781 ctgcagccgc agtttggaaa acagtaggat tagttggtta ggaattaata ggagactaac
      841 tgtctcctat tgattccaat gattaagcta aggaggaatt ttaagaaaat aatgaaatac
      901 aataatttaa aaccgaaatt acttaatgag aacatagaat atataagaga agaaaattat
      961 ggatatttaa ccatagttcc caaaaatgga actgaatcga aagataacgt tttcaatcca
     1021 acagtaagat atatattggc taactgtact ggaaaaaaca gtgtaaaaaa aatagcgaat
     1081 ggtttaaaaa agaagtttcc tagtttagaa t
//
LOCUS       DAZUMB010000281         1109 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000281 DAZUMB010000000
VERSION     DAZUMB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.294"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1015
                     /locus_tag="ACG80L_002963"
     CDS             <1..1015
                     /locus_tag="ACG80L_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5025115.1"
                     /translation="DRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLLKARLEIAIS
                     KRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMGFNKQLGKNN
                     PNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAESLGAQEGMK
                     IIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFETIMTNDSIAT
                     FVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDSYYVNAEQEL
                     YTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYESSPSTITKD
                     WLE"
BASE COUNT          459 a          164 c          182 g          304 t
ORIGIN      
        1 ggatagagtt tacaacgaat tattttatca atttccaaaa gtctttattg tttctgacga
       61 atacaaaaaa atgaaagatt caactaagat tgcctatatg cttttaaaag caagattaga
      121 gatcgcaatc agcaaacggc aaatcgatga agaaggtaat gtttatttta cttatacgac
      181 aaatgaacta tgtagagtat taaactgcca aaaacaaaaa gcgatagcaa tcaaaaaaga
      241 gttggaatcc tttggtttat tattacaaaa gcagatggga tttaacaaac agttagggaa
      301 aaataatcct aatagactat atctagcaga attaaaagtc tcagaaaatg atatctactt
      361 actcgaaaaa tttgatagag agaataggga aaacgttgat aaatcagagg gtatgaaaat
      421 catacccacc ctcgacgaaa aatcagacgc tgaatccctt ggggctcaag agggtatgaa
      481 aatcataccg tgccaaaacg ttgataaatc agagggtatg aaaatcatac cagaacttaa
      541 taataatata ttagacacta atagacacaa tatagacact gaaaaagacc gcctacaaga
      601 tcaattgttg ttagacaatt ttgagacaat tatgacaaac gacagcattg ctacgtttgt
      661 ccctgaacga gtattaaatt tgataaaaac attttcttca agttacagtg aagctcaaaa
      721 aaccgtccag actattcata atgcaaagaa aaaagctgaa atagaaagtg gtatttcgat
      781 agtttttgaa gaactcgata gttattatgt caatgcagaa caagaattat acacgacact
      841 gttaaaagcc tatcaaaaat taaaaaccga aaaagtcgaa aatatccaga acctgatttt
      901 tgtctatgta aaaaattggt ttatcgaaaa accaatagct gctaaagtat caagtgaaaa
      961 acgtttgaat tatgaaagct ccccaagcac tattacgaaa gactggttag agtgaaactt
     1021 tactgacaaa gtgattgttg cttcatggag taacattaaa attaaaaatt ttttattatt
     1081 ctaattatat tacgaatatt acaaaataa
//
LOCUS       DAZUMB010000282         1106 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000282 DAZUMB010000000
VERSION     DAZUMB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.295"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG80L_002964"
     CDS             <1..1087
                     /locus_tag="ACG80L_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5025116.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DAZUMB010000283         1087 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000283 DAZUMB010000000
VERSION     DAZUMB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.296"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..932
                     /locus_tag="ACG80L_002965"
     CDS             <1..932
                     /locus_tag="ACG80L_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025117.1"
                     /translation="LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLN
                     GLISYIKSLITGETNTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLIL
                     IFMGLKRIELLALSGAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILA
                     LLMSNLGITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRI
                     AASLHSDILTIYIEVGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILY
                     LTDNTLTYPDTQMIFLLVPMIVSWVDNQQRISE"
BASE COUNT          363 a          106 c          193 g          425 t
ORIGIN      
        1 ttcttgcata tggttacgca tgtacagcat tttacttatt taaggcagaa ggaattgatt
       61 ttattttttg gtcaggtgtt atttcatata tgagtggttc agttttttat ttaattgtat
      121 cttatggttt aaatggttta atttcttata taaaaagtct tataacaggt gaaacaaata
      181 ctgcaaattt tgctatggag gtacatgatt taacttttgc gatgggggtt tttttccttt
      241 attacgtatt ttttgaaaat aaaaatacaa aaaaacataa gataaaaatt atcgtatctt
      301 taattttaat tttcatggga ttgaagagaa ttgaactgtt agctttaagt ggtgcaattc
      361 tagtgtatta tgttttatta aagtggggaa aaaagattca aatccgtgca ttagtgtgta
      421 ctatttgtgc aactgttatt tcgttagtgt ttatttacat tatagataca ggaattttgg
      481 cattgcttat gtcaaatttg ggcattacag atgacggtgc gaggctttca ttttatctat
      541 attcatctaa attttatgaa cttggtttag gatatgtagg aacaggatgg acatggtttt
      601 caaaatactt ttttaatctt tatttatcgc actttaggtt aaatggtgga agaattgcag
      661 caagcctaca cagtgatata ttaactattt atatagaagt tggatgtata gtttttatta
      721 tatggatgtt ttggttattt tttgtgaaac cgataagact aactaaaaaa tttggattga
      781 aaacagggga gtgtgtatta ttattaacta tatatatgtt tatattatat ttgacggata
      841 atacgctcac ttatccagac actcaaatga tttttctctt agtacctatg atagtttcgt
      901 gggttgataa tcaacaacga atatcggaat agatagctaa agtatatttt aattatgaat
      961 ttataattag ttgtaaaagt taatatttaa cggacaggaa gtagggtgaa aaaatttgaa
     1021 aaaaatttta gtgttagcaa atacatatta tcaaataata gtagcaatac aaatgaaaaa
     1081 tacaatt
//
LOCUS       DAZUMB010000284         1051 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000284 DAZUMB010000000
VERSION     DAZUMB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.298"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG80L_002966"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG80L_002966"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5025118.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAZUMB010000285          997 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000285 DAZUMB010000000
VERSION     DAZUMB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.299"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACG80L_002967"
     CDS             complement(26..913)
                     /locus_tag="ACG80L_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5025119.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DAZUMB010000286          994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000286 DAZUMB010000000
VERSION     DAZUMB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.300"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACG80L_002968"
     rRNA            complement(<1..>994)
                     /locus_tag="ACG80L_002968"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAZUMB010000287          986 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000287 DAZUMB010000000
VERSION     DAZUMB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.301"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACG80L_002969"
     CDS             <1..704
                     /locus_tag="ACG80L_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5025120.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAZUMB010000288          963 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000288 DAZUMB010000000
VERSION     DAZUMB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.302"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..415
                     /locus_tag="ACG80L_002970"
     CDS             5..415
                     /locus_tag="ACG80L_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5025121.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            429..785
                     /locus_tag="ACG80L_002971"
     CDS             429..785
                     /locus_tag="ACG80L_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5025122.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          328 a          153 c          189 g          293 t
ORIGIN      
        1 gaaaatgata aaagtatata cttcgcctag ctgtacttcc tgtagaaaag caagagcatg
       61 gttgcaagcg aatcaacttg aatttgaaga gaagaatatt tttgccgaac cgttaacaga
      121 aagtgagatc aaaaagatct tggcagcgac tgaaggagga gttcaagata ttatttccac
      181 tcggtcgaaa atttacgaaa agctgggcat agattttaac gaattaactt taaagcaaat
      241 gatcgtctta ttcaaagaat atccttcatt attgagacga cctattttga ttgatgaaaa
      301 aagaatacta atcggattta atgaagatga gatccggtca tttgtccctc gtgaaattcg
      361 atgtgttgcc agaaatctga tctatttaca aaacaataac atcttaaatt catagggagg
      421 gagccttcgt ggaagtgaaa gtctatggat cagcgcactg tgtaacaagt ctgaaggcga
      481 aacaatggtt agaacaccat caattgcctt atcaatttgt tgacttagaa gaacgcgatc
      541 taagtacaga agaagcagaa gaattatgca gttttgaaga agtgcaagca gaacagctat
      601 ttgcgacttg gtctgaggct tttcacaaca ttgcgattga cctttcctgt gtaggaaaaa
      661 aacaactgat ttttttatgc acacaacata ccaatctcct cagacgtcct ttgattatta
      721 tcgatgaagt tctatttatt ggatacaatg agcagttgat ggaacagctt atcttaaata
      781 aatagacttt aactgtttta atcaagtcac tgagcatttc atacagttgt aacagtcgaa
      841 cacttagcat caagcgtgtt atgtgttctt tttttgactt tatttttcta ctatagagac
      901 atgctcaatt tatgtggtca aaattcaaat ctaggtgggg atatagagtt aaaaatatat
      961 tga
//
LOCUS       DAZUMB010000289          960 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000289 DAZUMB010000000
VERSION     DAZUMB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.303"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..>960
                     /locus_tag="ACG80L_002972"
     CDS             131..>960
                     /locus_tag="ACG80L_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5025123.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          337 a          141 c          165 g          317 t
ORIGIN      
        1 gaaatatcta cacagacaaa atgatagcgt aaaatatttt gtgtaaatag atatttaggg
       61 tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc acaaccgaaa ttttatagga
      121 ggactttctc atgcccaatt ttactacaga aattatggaa acactaatca ataaaggtga
      181 tttagatgaa ttatttcgat gtcatttaga attagctgtt aattcattat tgcaggctga
      241 attaacagct tttcttgatt atgaaaaata tgatcgtgct gggttcaatt caggtaattc
      301 ccgtaatggg aattattcac gttcattcag aacagagtat ggagaattaa atttggtaat
      361 tcctagagat agaaatggta agttttctca acaaacttta cctgcttata aaagaaccaa
      421 tgactcttta gaaaccacta ttattcagct atttcaaaaa ggaattacta tgtctgaaat
      481 ctctgaattg attgaaaaaa tgtacgggca tcactatacg ccacaaacca tttcaaatat
      541 gactaaaata gtttctgaag atattattgc ctttaaagaa aggtccttag aatccagata
      601 ctctgtcatt tttatggatg ctactcacat tcctttaaaa agacaaactg tttcaaaaga
      661 agcagtttat attgtaattg gcattcgatt agatggaacc aaagaggttc ttgggtttag
      721 tcttgctcca actgaatctg cttatgtttg gaaagagatt cttcaagacc taaaagaccg
      781 tggtttaaaa gaggttttat tagtcgtaac tgatggttta agtggcatta acgatagtat
      841 tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat
      901 cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta
//
LOCUS       DAZUMB010000290          959 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000290 DAZUMB010000000
VERSION     DAZUMB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.304"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACG80L_002973"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACG80L_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACG80L_002974"
     CDS             complement(570..>959)
                     /locus_tag="ACG80L_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5025124.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAZUMB010000291          938 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000291 DAZUMB010000000
VERSION     DAZUMB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.306"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..892
                     /locus_tag="ACG80L_002975"
     CDS             152..892
                     /locus_tag="ACG80L_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025125.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          350 a          166 c          187 g          235 t
ORIGIN      
        1 tatcagagag acaacgaatt aagtcttatt actatatata tatttatgaa attcatcgct
       61 attttcttaa aataatcagc gcattgctac tgaaaacaat gaatctgtgc tattattgag
      121 aaaagttcca tttttgagaa ggatggtttg aatgaaagaa atcagacgaa aacatttccg
      181 ttttcctgcg tacgatgatg agttaggcgt aaaattaacc aacgacaaga agcgagtatt
      241 atttgacgga caagaagatc ttttgactga acagaatcag tccccgtttg aacagatgaa
      301 agaatttcat ttcacgaaca cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa
      361 tggattgaag tcagctccgc gtaaggaaaa tttggaaaag cataaaggaa aattaccgga
      421 ttataccggc cattaccaat cagaaatcac atcgacagga cgaaaaaaac tttttggtga
      481 ttcctctcgt tctgtcagac aaagtgaaac aaggacagag tccaagcata ctacacgaaa
      541 cgcatctgtt gacacgaagg tgcgggagcg ttcttatttt gttcctaaat atgtacccgc
      601 atcgattatc ccagacccta gagaaagcaa aatttctgag gatcatttaa tcgaatcgat
      661 gaaaaaaacg gatgactctt atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa
      721 aagaaatggt gagccgtctg tacgaacatt caatcgaaca aaaacgttag aggaaacaaa
      781 gcagtcagaa gaaaaaaaat cgaaaaaaag ccatggtatc ttagaacgct cattacaagg
      841 attgatcgat gatcaaggaa actctttggg agaaaatagc tattttaaat aagtgatata
      901 caaatttata ttcggctaca gttggaaagg aaacgctg
//
LOCUS       DAZUMB010000292          930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000292 DAZUMB010000000
VERSION     DAZUMB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.307"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..>930)
                     /gene="menA"
                     /locus_tag="ACG80L_002976"
     CDS             complement(155..>930)
                     /gene="menA"
                     /locus_tag="ACG80L_002976"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS5025126.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          330 a          193 c          161 g          246 t
ORIGIN      
        1 taagcgactt tttcacaacc tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat
       61 aagcccaacc aaacgaaaaa tataaggagc tatttttatt tggctgttct tattacttga
      121 tgcagggcag ttttttcaca acctcttcat gctgttaaat ggcttgcata atgattgtta
      181 gtaatagccc caatacataa ctggcattga ataagaccat atttttgatg gaatagctga
      241 aactgatagg ctggggtaac gtttgcttga attgatccaa atgcttcttc acaattggca
      301 aggtcaagaa ggtgaccaag atcggccatt catagatacc gctcaacaac cccattaaaa
      361 tcacgaaata gcaagcatac atcaatagct ggaataagac tgttcccgca ggacgtccga
      421 tataaaaaac tagtgtatag cgatgatttt caatatcacg gtcaagatcc cgcaaattat
      481 tagccaacat gatattggca atcgtaaaca ccaagggtaa ggaagctaat acgattgctg
      541 ctatagccca aagatttcct accaaatcaa attgtccaga tgaaaagttg actgtcagat
      601 catagacatc tgaaccgaat gtattgacat aaacgaccat ggcaaaaatt ccaaggccca
      661 tagtcacccc actgaaaatt tcccctaaag gcattcgaga taaaggaatc ggtccgaatg
      721 tatagaatac tccaataaaa caacagataa atcccatcaa aagtaaaagc caacccgtat
      781 tgatcgttag aataacgccg ataacaaaaa caaatgcaat catagctaaa atcatgtttc
      841 gaatcaatac aggagataga tttctgcggc caatgacatt ttcttcatac ttatatactt
      901 cagaatgagc cttttcataa tccatgtaat
//
LOCUS       DAZUMB010000293          927 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000293 DAZUMB010000000
VERSION     DAZUMB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.308"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            295..927
                     /locus_tag="ACG80L_002977"
                     /pseudo
     CDS             295..927
                     /locus_tag="ACG80L_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          311 a          174 c          174 g          268 t
ORIGIN      
        1 taaatctcga aaatggttag atgtaatcat caggaaaaac aatcttaaac gaattactat
       61 tcatggtctt agacacacac atgctagctt gcttttagca agctagtgca aatattaaag
      121 atgtgcaaga gcgtttaggc cactcttcta ttcaaatcat tatggatcta tatattcata
      181 ttacagacaa aagaaaagaa gaaaccgtag cgcaattcgc aaaatatagg ctctataatt
      241 tatgggactg atgaatcaaa attgattctt cagtcccatt gttattttag gtatatgatt
      301 cgtacattga aagagttgaa aaactatctt ccgaagccgt caaaaaggac cctgccaaaa
      361 gtattgatgg tggatgaatt tcgttcgcac gcttcaattg tggataaaat gagctttatt
      421 tgtgcggatg gcgagactgg aaaactcatt gatattttgc ctacacgaaa attgtctcga
      481 ttaacacact attttagtcg ctgtgcaaat cctgaagcgg tggaatattt agtgacagat
      541 atgaacgccg cctacttcca actgacaaaa cgtgtcttac caaatgcgaa agtcgtcatt
      601 gattgttttc atatcatcaa acatttgaat caggcgttca atgattttcg tgtacaagaa
      661 atgaaaagat tacaccagtt agggcaacga cgggaagccg agaaagtgaa aaagaactgg
      721 cgctttcttc taaaaaaccg tcaaaaaatc aatcactatg attacaaaac atggcgcagt
      781 tttcgtgcac ctaaataccc gctactcact gaagcaatga tgattgatcg attgctggca
      841 ttttcaacgc ctttaaagga cgcctatact tttttccatg agctgaccga agcctttaga
      901 gacaaggaat cagacttatt ttttacc
//
LOCUS       DAZUMB010000294          916 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000294 DAZUMB010000000
VERSION     DAZUMB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.309"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..356
                     /locus_tag="ACG80L_002978"
     CDS             87..356
                     /locus_tag="ACG80L_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5025127.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="ACG80L_002979"
     CDS             349..606
                     /locus_tag="ACG80L_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5025128.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          352 a           99 c          174 g          291 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt actaaaggat gacgcttata tatatgactg aatagaataa
      901 gcaataggtt taataa
//
LOCUS       DAZUMB010000295          904 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000295 DAZUMB010000000
VERSION     DAZUMB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.310"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..888
                     /locus_tag="ACG80L_002980"
     CDS             265..888
                     /locus_tag="ACG80L_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5025129.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DAZUMB010000296          899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000296 DAZUMB010000000
VERSION     DAZUMB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.311"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            229..882
                     /locus_tag="ACG80L_002981"
     CDS             229..882
                     /locus_tag="ACG80L_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5025130.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          371 a           84 c          164 g          280 t
ORIGIN      
        1 tctttttgag ttgaaacgaa gaaaaagtgt tagtagaaaa tcgccttcga ttttccaaca
       61 acacaaaata ttatagaacc ctttattcta aaggatttca aggtgtctgt ttgcatttat
      121 tagaaaaaag aaaaagtatt ggcaacagaa ggtgtcacca acactagata ttatagagcc
      181 ataatatgct gtataatatc tagtgtatag gcttaagtgg aggtatatat gaaaatatta
      241 actgttgaag atgataatat gataagagag ggaataagtg aatatctttc agaatttgga
      301 tatactgtta ttcaagctaa agatggaaga gaagccttgt caaaatttaa tagcgatata
      361 aatttggtta tcttagacat tcagatacct tttataaatg gtttagaagt gttgaaagaa
      421 attagaaaga aaagcaattt accaattcta atcttgactg catttagtga tgaagaatat
      481 aaaattgatg catttactaa tttagtagac ggatatgtag aaaagccatt ttcattgccg
      541 gtcttaaaag ctagaataga ttctttaatt aagaagaatt atggacattt agagaagttt
      601 gaatataagg atctatcggt taattttaat agctatacag ctaaaataaa tggtgaagaa
      661 atagatgtaa atgcaaaaga actagaagta ttaaaatgtt tattggataa taatggacaa
      721 gtgttaacaa gaatgcaaat tattgattat gtatggaaag atagcgaaga aactccttat
      781 gatcgagtaa tagatgtata tataaaagaa cttagaaaaa aattacaatt agactgtata
      841 actactataa gaaatgtagg atataaattg gagagaaaat gaaaaaatta aagatattt
//
LOCUS       DAZUMB010000297          888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000297 DAZUMB010000000
VERSION     DAZUMB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.312"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..358
                     /locus_tag="ACG80L_002982"
     CDS             <1..358
                     /locus_tag="ACG80L_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS5025131.1"
                     /translation="FFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRRFPEGEE
                     VWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDDWQKVMA
                     AEVIATYLDGDLVYKK"
     gene            406..>888
                     /locus_tag="ACG80L_002983"
     CDS             406..>888
                     /locus_tag="ACG80L_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5025132.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     D"
BASE COUNT          261 a          148 c          205 g          274 t
ORIGIN      
        1 cttctttaat tattgtcgat tagaggaaag tggtgtaaca gtcactagcg gtacagattt
       61 accgctgttt attactgata ttcctgaatc gattataaga gcagtattcc gtcgttttcc
      121 tgaaggagaa gaagtctggg agaaagaaac aagcttcacg acaaccgagc tgctgaaaag
      181 ttttacaagc aacagcttca aagtaaatgg tttatcggag tacggagaaa taaaagtagg
      241 gaaaagcgcc agcttagctg ttttcagcga ggacctctgt actgatgact ggcaaaaagt
      301 gatggctgcg gaagttatcg ctacctatct tgatggtgac ttagtttaca agaaataatg
      361 cttttcgata taacgttata aaacgaatta aagagaggtt aaaaaatgga aacaatgaaa
      421 agaaaattca ccttcccaca tagttataca ttgattttta tcttaattat tttggctgca
      481 attttaactt ggataattcc tagtggtcag tttgagacag ttgttgaaaa cgtcgcaggt
      541 atggaaaaaa caacggttgt tccgggaacc tatcaccaaa tcgataaggc agattatgga
      601 caaggttttt cctctatact aatggcacca gctgaaggga ttatttcagc tgttgaggtt
      661 gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta tcctaaaaac gggagccatc
      721 gatcgagggc tgtttacctt agcaaaagtt ctgggagaca aggggatctt agttattccg
      781 atgtcgatga ttctattcag cttaggagga tcaacctttg gcatgagcga ggaagtcatt
      841 cccttatttg cggtgtttat ttctctgatg ttttctcttg gctatgat
//
LOCUS       DAZUMB010000298          885 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000298 DAZUMB010000000
VERSION     DAZUMB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.313"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..>885
                     /locus_tag="ACG80L_002984"
     CDS             63..>885
                     /locus_tag="ACG80L_002984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFS5025133.1"
                     /translation="MNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIY
                     LTKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDM
                     PLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVST
                     RMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLK
                     KILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFS"
BASE COUNT          323 a          113 c          151 g          298 t
ORIGIN      
        1 aaatagagag agattttcaa atagaatttg actattatta tcttcaaata acgtctatct
       61 ttttgaattc ttggatttat caaatccaat taacatcgga cgataaatta tattccttca
      121 tcaatgatat taatagttgg tgtagtcgag aagttcaatt agtcaatcgt tttattcaaa
      181 taggggatat ttatttaaca aaaattcaaa aaaatgtttt ggttatttta tttaagttaa
      241 tgtgtagtaa agaatttcag caaaaacgtt tagcactgat tttggctcat ggagatctaa
      301 cggcaacctg tatagcaagt actgctaata ggatcgttga gagatatata tacgacgcca
      361 ttgatatgcc actaatgact tcaagtagtg agattgcaga caaaattaat agatacttac
      421 aagagtgtac cggatatgaa gaaattattt tacttgttga tatgggttca ctggagaaaa
      481 tttatctaaa tttggaacta gacgctcaaa aaaaattcgg actactaaac aatgtttcaa
      541 caagaatggc tattgatgtt gcaaatcaga tattattaga tttaccgctt aaacagattc
      601 ttgaacaagt aaaagaaaat caaaatgtgg attatattta tgaatcatca aatattaaga
      661 aggatatgat tgtctgttct tgtgcaagcg gattaggaac ttcgtttaaa ttaaaaaaga
      721 tacttgaaga tagctttcca agggatactg atcttgtcat agaaacgtgt gactattact
      781 ctttagtgaa tgaaaactat ttatcggagc ttcgtaaaga gaacaatatt ctttttattg
      841 ttggaacact aaatccacat ctagaagatg tagaattctt ttcaa
//
LOCUS       DAZUMB010000299          871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000299 DAZUMB010000000
VERSION     DAZUMB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.314"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG80L_002985"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG80L_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5025134.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          251 a          184 c          134 g          302 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa a
//
LOCUS       DAZUMB010000300          855 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000300 DAZUMB010000000
VERSION     DAZUMB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.315"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..>855
                     /locus_tag="ACG80L_002986"
     CDS             26..>855
                     /locus_tag="ACG80L_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFS5025135.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          295 a          126 c          156 g          278 t
ORIGIN      
        1 cattcacaga aaagaggact tccatatgaa cgattttact acagaaatta tggaaatact
       61 aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg ctattaacgc
      121 tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata gagctggatt
      181 taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag agtacggtga
      241 attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa ctttacctgc
      301 ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc aaaaaggaat
      361 tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt acaaaccaca
      421 aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta aagaaagaac
      481 tttagaagct aaatactcag tcatttttat ggatgctact catattccct taaaaagaca
      541 aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg gaactaaaga
      601 ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag agattcttca
      661 agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg gtttaagtgg
      721 tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca
      781 tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga
      841 tttcaaatta gttta
//
LOCUS       DAZUMB010000301          850 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000301 DAZUMB010000000
VERSION     DAZUMB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.316"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..293
                     /locus_tag="ACG80L_002987"
     CDS             99..293
                     /locus_tag="ACG80L_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025136.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..>850
                     /locus_tag="ACG80L_002988"
     CDS             562..>850
                     /locus_tag="ACG80L_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025137.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSK"
BASE COUNT          352 a          103 c          141 g          254 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa
//
LOCUS       DAZUMB010000302          811 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000302 DAZUMB010000000
VERSION     DAZUMB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.319"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG80L_002989"
     CDS             <1..672
                     /locus_tag="ACG80L_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5025138.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZUMB010000303          803 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000303 DAZUMB010000000
VERSION     DAZUMB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.320"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..>803
                     /locus_tag="ACG80L_002990"
     CDS             129..>803
                     /locus_tag="ACG80L_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5025139.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNA"
BASE COUNT          240 a          155 c          147 g          261 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagcag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac gct
//
LOCUS       DAZUMB010000304          791 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000304 DAZUMB010000000
VERSION     DAZUMB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.321"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..708
                     /locus_tag="ACG80L_002991"
     CDS             40..708
                     /locus_tag="ACG80L_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5025140.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          221 a          131 c          138 g          301 t
ORIGIN      
        1 agaagttgct gaaaattttc aaaagaaaag aggctagaaa tgaaaaaatt aaatttattt
       61 acagaattgt gcggaatcat tttaggcgca ggaatctacg gattagctgt gacaggaatc
      121 aatattccat ctaagctggc agatggtggg gttactggta ttgctttgct tttgcataat
      181 ctttttggat tcgctccttc tgtcacttca ctgcttttta atttaccttt attgcttatc
      241 tcattgtttc tttttggaaa acacgctttt ctccggacga tcgtcggaac atttgcgcta
      301 gttttctttt tacatttatg ggagagcctt catatacatt ttgctgtaga tagtctactg
      361 atcaatagtt tgatgacagg tattctttct ggcattggtt gcggtctcgt ctttcgattc
      421 ggcggaagca caggcggaac agatatcatt tatcaagcca ttgaaaaata ttttcatatt
      481 aagattggca aaagtctttt cttgattact tttggtattt tagttgtctc cttgctttat
      541 ttggatctca cacattttct gtatacatta cttagctgtt ctattctatc ttatacattg
      601 aataaagtga agtatctgga aattggatct gttttgcccc aattggaagc aaaacagact
      661 acagaaacat ttgaaccact tgatcctctt gaacagttag agcactgatt cttatttttc
      721 actactttat ttaatttaca gaaatatcat tatgtaaact aaaatgatat tattcagtaa
      781 aatcttcttt a
//
LOCUS       DAZUMB010000305          750 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000305 DAZUMB010000000
VERSION     DAZUMB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.323"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..500)
                     /locus_tag="ACG80L_002992"
     CDS             complement(75..500)
                     /locus_tag="ACG80L_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5025141.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWSYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          216 a          138 c          152 g          244 t
ORIGIN      
        1 tagtgacccc caaaagttag acttttttgg agtggagaaa tccactcttt ttttcatgct
       61 ctaaaatgat atttttacac aaccgtaacg gttgttttac gaaattggac gggactttgc
      121 cagtttaatt tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct
      181 gattttaatt cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag
      241 ccaaaaaaat tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga
      301 aagattttgt actccttcaa tttgtttcta taagctttca tttgatacga ccaaccttga
      361 tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa
      421 ccttccatta ccgcacgtgt ttctgaatgg tggtagcgac cgatttgaac ggctagggcg
      481 gaaatacggt gcaaatgcac ggtggtttca tgcaatgctt acagggcgta ttttgtaagg
      541 gtatgtaacg gagataatcc cgacggtaat ggatgtgaga ggccaagccg acgctgacgg
      601 tagggcaaac aaacatagtt gcactctagc gatcgttatt tatttttaac ggtcaaacta
      661 gggtacaact atgcttaaaa cacttcgcct caacttctgg tagcaaaagt gattttgaaa
      721 gtcaaaatat tttttatgag ttatttataa
//
LOCUS       DAZUMB010000306          746 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000306 DAZUMB010000000
VERSION     DAZUMB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.324"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..161)
                     /locus_tag="ACG80L_002993"
     CDS             complement(<1..161)
                     /locus_tag="ACG80L_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5025142.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
     gene            254..746
                     /locus_tag="ACG80L_002994"
                     /pseudo
     CDS             254..746
                     /locus_tag="ACG80L_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
BASE COUNT          253 a           93 c          128 g          272 t
ORIGIN      
        1 tattctaagg ctgattttcc ttgcttgaag aacagatata ctttatggat cgtttgtctt
       61 gaacgattca agcaatgggc agttttagca acagattgat tcattttgaa ataagactct
      121 attattacaa gttcatccgt tgtaagatgg gtataggtca tttgtactca ctccttataa
      181 ttctttggtc ggaactattt tgagtgtagc acaaatgact tttttagttg tctagcttaa
      241 ttttacaatc ggcgttatat taaaaaatga atttggaata gaagttaaag attattttat
      301 tgacttagat ttattacagc taataaacag tgtgaaaaga atgtatagtg gagacgacaa
      361 cggtgttgat ttatacaaat ttgttaccaa acatatattt gttaatctta atattgacga
      421 gaaaaattac ccgtctattt ttaagcacta tctatttttt attaacgaag tagaagatcg
      481 gataaaaaat aattcattat ctagtagtgt atctagtttt aagaagatgt ttaaatctcg
      541 agatggtatc acacttacta cagcacataa agttaaagga aatgagtatg atactgttat
      601 cgctattaat cttctaaacg ggaaaattcc tcattggaat gaaattttta atgtttctga
      661 taaaggaatt agttcagcga gaaaactact atacgtaatt tgttcaagag caaaaaataa
      721 tttatttctt ttctcagagg tgggtt
//
LOCUS       DAZUMB010000307          733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000307 DAZUMB010000000
VERSION     DAZUMB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.325"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACG80L_002995"
     rRNA            <1..>733
                     /locus_tag="ACG80L_002995"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAZUMB010000308          692 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000308 DAZUMB010000000
VERSION     DAZUMB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.327"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..301
                     /locus_tag="ACG80L_002996"
     CDS             <1..301
                     /locus_tag="ACG80L_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5025143.1"
                     /translation="RVSESEFAKLKQSAEALQMSVPAFVKAKAQNARLVTPKVAPDIA
                     QAIARDLAKAGGNINQIAKWCNTHQHDVDPGDAQRLSENLKIMQKELQKIWQQLK"
     gene            283..>692
                     /locus_tag="ACG80L_002997"
     CDS             283..>692
                     /locus_tag="ACG80L_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5025144.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICL"
BASE COUNT          241 a          164 c          147 g          140 t
ORIGIN      
        1 tcgagtgagc gaatccgaat ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag
       61 tgtgccggct ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc
      121 gccagacatt gctcaggcaa tcgcgcgcga tttagccaaa gctggcggga atatcaatca
      181 aattgccaag tggtgcaaca cacaccaaca cgacgttgat cctggcgacg cccaacgctt
      241 atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag atatggcaac agttaaagta
      301 agccgaacca cttcttgtag ccgagcgatc aattacgccg aaccacgagc aaccgtcaaa
      361 acagggataa actgtgacat tgattatgcc aaaagcgaaa tgaaacaaat tcgcatgctg
      421 tatggaaaag acgatcgtgt acaagcacac ttattgatcc agtcttttcg accaggagaa
      481 attaccgctg aaaaagccaa tcaactaggg gcagagtatg cgaaaaaaat agcccctgac
      541 caccaaatcg cgatttacac acatacagac aaagaccata ttcataacca cattgtcatt
      601 aactccgtca atctggaaac tgggaacaaa tttcaagccc atggtcaagc atttttaaac
      661 aaatgttatg acctaaatga cgagatttgt ct
//
LOCUS       DAZUMB010000309          687 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000309 DAZUMB010000000
VERSION     DAZUMB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.328"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(376..600)
                     /locus_tag="ACG80L_002998"
     CDS             complement(376..600)
                     /locus_tag="ACG80L_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025145.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          249 a          103 c          123 g          212 t
ORIGIN      
        1 cgtttattgt gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc
       61 gcagattcta tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagctac
      121 ttgagatttt gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc
      181 ttgttttcgt aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg
      241 gtgacaacag tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc
      301 aataaattgt tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt
      361 tgaaatctgt gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa
      421 gatgaataaa aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg
      481 ttctgtcata gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat
      541 gatttctcga aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat
      601 tgaatagtca tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag
      661 gaactgtacg ttttttagaa taaaaat
//
LOCUS       DAZUMB010000310          671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000310 DAZUMB010000000
VERSION     DAZUMB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.329"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..547
                     /locus_tag="ACG80L_002999"
     CDS             <1..547
                     /locus_tag="ACG80L_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS5025146.1"
                     /translation="SEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSL
                     PEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSE
                     TKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKK
                     YAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          235 a           82 c          121 g          233 t
ORIGIN      
        1 gtctgaaaaa aattattcta tcattgtttt tgaaaagtta gcacaaacgt ctataacatt
       61 aggaatggat ataatagaag cgtatcaaag tagagatgct ttgatacaag aaaacgaatt
      121 agctgtatcg ttacctgagg tcttaaaagt gagggattct gggattgttt attatactaa
      181 ggaaatcgga aaaactaaga tagagcatct ttctccctta atttcttctg tcgttcaatt
      241 catcggctta aatatctata aaagaattac tgtaaaggaa atagctaatt atttttctgt
      301 aagtgaaact aaattaagag aaagctttaa gaatgaaatg catattacga tttataatta
      361 tatctctaag cgaaaaattt ctacggctaa aataatgttg aaatcaaacc atactatcag
      421 tgaagtttct ttaggtttag gattttcaga ttcttcacac ttttcgagag tattcaaaaa
      481 gtacgcaggt gtatctccta agcaatatca attaggttta gtggacaatt tcaacaacat
      541 tagctgagag gttttatttt attatgagac tatatcattt acgtagtttg gaggagttga
      601 ggaaattgga ttttaacaaa aaattttttt gggaaatttc tgatttgagt tgtttcgtag
      661 aatctgagcg t
//
LOCUS       DAZUMB010000311          670 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000311 DAZUMB010000000
VERSION     DAZUMB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.330"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>670
                     /locus_tag="ACG80L_003000"
     CDS             <1..>670
                     /locus_tag="ACG80L_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5025147.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQS"
BASE COUNT          219 a          113 c          136 g          202 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg
//
LOCUS       DAZUMB010000312          668 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000312 DAZUMB010000000
VERSION     DAZUMB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.331"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>668
                     /locus_tag="ACG80L_003001"
     CDS             51..>668
                     /locus_tag="ACG80L_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS5025148.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKE"
BASE COUNT          247 a          110 c          132 g          179 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaa
//
LOCUS       DAZUMB010000313          644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000313 DAZUMB010000000
VERSION     DAZUMB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.332"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(274..465)
                     /locus_tag="ACG80L_003002"
     CDS             complement(274..465)
                     /locus_tag="ACG80L_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025149.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          212 a          126 c           87 g          219 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactacaa taatattttt
      601 ctaacttatc gccttcaaag aaatagtttc ttatttttca ctaa
//
LOCUS       DAZUMB010000314          619 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000314 DAZUMB010000000
VERSION     DAZUMB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.334"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..321
                     /locus_tag="ACG80L_003003"
     CDS             <1..321
                     /locus_tag="ACG80L_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5025150.1"
                     /translation="FFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKF
                     YEVNPVLEEPVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFG
                     IHYD"
     gene            389..>619
                     /locus_tag="ACG80L_003004"
     CDS             389..>619
                     /locus_tag="ACG80L_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5025151.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHK"
BASE COUNT          216 a           93 c          143 g          167 t
ORIGIN      
        1 ttcttttcta tagaagactt ttccgatgaa gaagtaaagc agtttttgat gaagcatttt
       61 tgtgagcgaa atgatgtatt gattgcaacc cgaggagaaa aaacggctat tggttatgat
      121 ggagcaaagt tttatgaagt gaaccccgtg ctagaagaac cagttgatac aatggcagcg
      181 ggagattctt tcttaacagc tttcctactt tattttctcg aaggggacga tatagtcacc
      241 gcgatgaaaa aggggaatga atttgctggg aagtcttgtc tggttgacgg ttcgttcggc
      301 tttggtattc attacgattg agcttgtttt aaattcctgc atttgataac ctaaagaaag
      361 aaatcccggt aaagtggagg ttaaaaaaat gctggataca aatgcacaat taccattgta
      421 cttacaatta aaagaaatat taaaaaataa aatcaataca ggggagtatc ccgagaatac
      481 gaaaattccc gctgaatcag agctgataga aagttatgag gtcagccgaa tcacagtgag
      541 aaaagcagta gaagaactgg tagaagaagg gtacctactt aaaaaacaag ggaaaggaac
      601 ttttgtcaac cggcataaa
//
LOCUS       DAZUMB010000315          606 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000315 DAZUMB010000000
VERSION     DAZUMB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.336"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..499
                     /locus_tag="ACG80L_003005"
     CDS             <1..499
                     /locus_tag="ACG80L_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HFS5025152.1"
                     /translation="YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIK
                     QPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEF
                     VYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCAN
                     ASEAV"
BASE COUNT          184 a          111 c          131 g          180 t
ORIGIN      
        1 ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc attttggatc tgttcaatcg
       61 agaaattctc ggctattctt gtggagaaca taaagatgcc gttctagtaa aaaaagcatt
      121 tagccgtatc aaacaacctc tgacagaggt tgagattttt catactgatc gtggaaaaga
      181 gtttgataac caagctattg atgaattatt aacaactttt gacatcaatc gatcattgag
      241 tcataaaggc tgtccttttg ataatgccgt agctgaatca acttataagt cgttgaaagt
      301 agaatttgtc tatcaataca catttgaaac cttacaacaa ttggatttgg agttatttga
      361 ctatgtcaat tggtggaacc accttcggtt gcacggtaca cttggctacg agacaccggt
      421 tggttaccgt aaccagagat tggcgcagcg aatccttgat aatgagctcg gatgtgctaa
      481 cgctagcgag gcagtctaac tttaactgtt agctcctgcc gaagatcgtc acatccgagg
      541 aggctcattg tcaaggacaa tcggaatagc atacggaaag aagttctgca ccttataaaa
      601 tttgtc
//
LOCUS       DAZUMB010000316          598 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000316 DAZUMB010000000
VERSION     DAZUMB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.337"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG80L_003006"
     CDS             73..234
                     /locus_tag="ACG80L_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS5025153.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>598
                     /locus_tag="ACG80L_003007"
     CDS             253..>598
                     /locus_tag="ACG80L_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5025154.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKN"
BASE COUNT          248 a           56 c          105 g          189 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatg
//
LOCUS       DAZUMB010000317          579 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000317 DAZUMB010000000
VERSION     DAZUMB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.341"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..421
                     /locus_tag="ACG80L_003008"
     CDS             20..421
                     /locus_tag="ACG80L_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS5025155.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            415..>579
                     /locus_tag="ACG80L_003009"
     CDS             415..>579
                     /locus_tag="ACG80L_003009"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5025156.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQF"
BASE COUNT          225 a           89 c           97 g          168 t
ORIGIN      
        1 aaaaaggagc atatttatca tgcaacaaaa ttacaatgag aaatctatac aacatcaatt
       61 tgatgcctac tgtaaaaaag tattacgaaa cgaaactaag tctattcgaa aacgaaacat
      121 taacataaca gataatgagg aaccattgac gaattcaaat gaaggtaagt actcccaaac
      181 tcattttgat gaacaagatg tctatttctt atttggaatg gaaatattaa tttcagatca
      241 aaagttatcg gcagccattg atcatttatc ggaaataaga agaaaggtta tcctacttta
      301 ttattttgca ggttttaatg atactgaaat cggtaaaatc ctcaatatga gtacaagtgg
      361 tatctggtat caacgaaaga aggcagttga acaattaaaa atggggtatg acttatggta
      421 gacaacccca aaaaacggca gtatccatta gttcctctat ccattatcca atctgctcta
      481 gaagaagatc caatttcaat caagatgata ctcaaacatt atcatagtta tatagctaag
      541 ttatgtttga caaatggatt taatgaggcg gggcaattt
//
LOCUS       DAZUMB010000318          575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000318 DAZUMB010000000
VERSION     DAZUMB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.342"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            374..>575
                     /locus_tag="ACG80L_003010"
     CDS             374..>575
                     /locus_tag="ACG80L_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025157.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLN"
BASE COUNT          229 a           67 c          107 g          172 t
ORIGIN      
        1 aggtgatcaa aatgggagta aacgaaatga tgaaagtcag attgagtttt taaatgatac
       61 cttcctcaga acttcaaaac gaattcaaag attcgttgac aaatcttgag caagatcatt
      121 taaaaaaata ttttctcttc atttgacgaa aaatgtttag aaatcttata taagtgaaga
      181 taaagtaatt ggtgtaatta attgtagaga atagcctttg agcaattgaa gcgatacaat
      241 tatttagaaa atgaacttcc tccagcatac tacatgattc cttacgggac gatgttaaag
      301 aagaaagcaa gaaattttag aagtattagt agataaacta atgggctttt ctcaatttaa
      361 gggagtagcc caaatgttta tcaaatatgg tgcagataca gtagcaacca tgtttacaga
      421 agaatatcaa gtagactatt atgaagtaat agataaatgt gtacattttg tttatgaaaa
      481 agtgtggaat tttatgaatt ttgcgataag tgaagaaatc ttacgagtac aagaaaacag
      541 aacaggaata tttaccagag acagaaagct taatt
//
LOCUS       DAZUMB010000319          555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000319 DAZUMB010000000
VERSION     DAZUMB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.344"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..>555
                     /locus_tag="ACG80L_003011"
     CDS             59..>555
                     /locus_tag="ACG80L_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS5025158.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          203 a          103 c           91 g          158 t
ORIGIN      
        1 agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg acttccctat
       61 gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt taaatgaatt
      121 attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat taacggcttt
      181 tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga gaaacggttc
      241 ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac ctagagatcg
      301 taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg atacattgga
      361 aaccactatt atccatttat tcgaaaaagg tgttacgatg tctgaaattg ctgatttgat
      421 cgaaaaaatg tacggtcatc actatactcc acaaaccatg tccaacatga ctaaagttct
      481 gactgaagaa gtaaatgcct ttaaatccag agccttaaat gataagtatg tcgctatttt
      541 tatggacgct actta
//
LOCUS       DAZUMB010000320          544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000320 DAZUMB010000000
VERSION     DAZUMB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.345"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..360
                     /locus_tag="ACG80L_003012"
     CDS             <1..360
                     /locus_tag="ACG80L_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5025159.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          202 a           89 c          116 g          137 t
ORIGIN      
        1 cgaaaccaac cagttgttaa accaaatgca atgcaaatca ataactcatt gaagcgatgc
       61 tttgtgaaag cattggctct acacggactg ggattatatg tatttcaagg ggaagatatt
      121 ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc tagagacgat tctagaagct
      181 ttcaatgagc agatgggtaa agatatgacc aaaaccttaa tcgaatatgt taatgagcag
      241 acagataaat tagggctctt agctgataac gttgaaacta ttgaacagtt aagctatgag
      301 caatgtgcct tgatggagcg agcaatagca gctaagagaa aagaattaga taagaagtga
      361 tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag ttcaaaggaa
      421 aagacataag cgcaaccatc aatgaggaag tgaacattga tcgactaaag acgatgtatg
      481 acggctacga tggtgatcga gtcattgaaa ttcgttttat tgatcctaga cgtttcaccg
      541 taca
//
LOCUS       DAZUMB010000321          526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000321 DAZUMB010000000
VERSION     DAZUMB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.347"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG80L_003013"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG80L_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5025160.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAZUMB010000322          524 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000322 DAZUMB010000000
VERSION     DAZUMB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.348"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          182 a           60 c           89 g          193 t
ORIGIN      
        1 acataacgtc atataacgtc atataacgtc atgtgacata attgcattgt atccgctttc
       61 tttgtgtttg tcaataggaa aaatttattt ctctaaaaaa tataaataat ctgcagtgaa
      121 acttgagtac atatactaat ttcgaaaaat gcctatgatt taaataatta tggtgctggg
      181 atgaaggttt ataagtttgt cttgatttgt ttagaagaaa tcgtttttgc acacagttga
      241 attaggaaac tactggagtg gaaaataaag tgatttgacg ggtagatggg tgcacttaaa
      301 tttgaaaatg gcttaaaatg ctttattttg aattgcagaa tttcttttag tacgcaattt
      361 atggtcttaa aactgattaa aaagtgatac attgatttta atacaattta tgatacaaaa
      421 aatgacctcc tttcttaaat aaaagaggtc attttgtttt gtctcataat cgattgtttt
      481 tgatacagta gttatattca gaaaaaataa agactcttct gagt
//
LOCUS       DAZUMB010000323          517 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000323 DAZUMB010000000
VERSION     DAZUMB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.350"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>517)
                     /locus_tag="ACG80L_003014"
     CDS             complement(<1..>517)
                     /locus_tag="ACG80L_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5025161.1"
                     /translation="GRLDDSPSHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKR
                     TRFLCYDCGQTFSAKTDLVDEHHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVT
                     RILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLY
                     KLTQYFLRFPR"
BASE COUNT          147 a          108 c          107 g          155 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctt
//
LOCUS       DAZUMB010000324          507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000324 DAZUMB010000000
VERSION     DAZUMB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.351"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          227 a           70 c           81 g          129 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactc
//
LOCUS       DAZUMB010000325          502 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000325 DAZUMB010000000
VERSION     DAZUMB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.352"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..426)
                     /gene="spxA"
                     /locus_tag="ACG80L_003015"
     CDS             complement(28..426)
                     /gene="spxA"
                     /locus_tag="ACG80L_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS5025162.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          142 a          103 c           86 g          171 t
ORIGIN      
        1 gtcttttcca agtttcgtat tatccttcta taatccagcc atcaattgtg cttgacgtaa
       61 ttctaattgt cgtacatcgc gcggtaggaa ccgacgaatt tcatcttcat taaaaccaac
      121 ttgcagacgt ttatcatcaa tcatgattgg acggcgaagc aatccaggat tgttttgcac
      181 aagttccaat aattcttgta acggcagatc atctaaatcc atgttcagtt tttggaatac
      241 tttggaacgt gtcgagatga tttcttctgt tccgtcttct gtcatttgta aaatcgcttt
      301 taattcagaa ctgttcaatg gttctgaaaa aatattacgt tcaacaaatg gaatctgatg
      361 ttcttgcaac cacgcacgag cctttctgca ggaagtacaa cttggggaag tatataatgt
      421 taacaacgtg caccactcct tttcgtgata agaatcactt ttttgtaaca gaagatcata
      481 ataatctccc atctgtttct at
//
LOCUS       DAZUMB010000326          502 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000326 DAZUMB010000000
VERSION     DAZUMB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.353"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            338..>502
                     /locus_tag="ACG80L_003016"
     CDS             338..>502
                     /locus_tag="ACG80L_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS5025163.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIAC"
BASE COUNT          177 a           89 c           74 g          162 t
ORIGIN      
        1 tctgtactga aatcaaggta ttattgggaa tcccagctta aatcatagat accgtaaggg
       61 attttattct ttatttaaaa ctttgcaaca gaaccttttg ccatatctac tccccctcta
      121 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
      181 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
      241 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
      301 gagtagtctc aaaactccca agaaatgagg actacatatg cgttcatcaa aatatactga
      361 acattataca gatactttta taacattcaa ggaaattatg agaactgttt ctaacatata
      421 ccacaattgt gtaccagata agattaaaaa ccgaagaaat actgatcaac tgaagcaacg
      481 cgatacagtg attattgctt gt
//
LOCUS       DAZUMB010000327          484 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000327 DAZUMB010000000
VERSION     DAZUMB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.354"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          177 a           65 c           58 g          184 t
ORIGIN      
        1 aaatattacg gaaaattata taaactaatt tagtgttttg tttgtaattc ctaattttgc
       61 tccactttta gataattttt aggtctattt aactcatcag acattctatg ctcatttttc
      121 tccaaatttg tttcaactct ttcgtactta attttgcgta tttgaaaatt cttcttttag
      181 agtactttta ttacgatact agtatgatat atatctatag caaacagtta aaatcttcct
      241 aaaataagcg aatagcattc ctaaaaccta taaaattttt agatgaagcg acaagtggac
      301 aattaataaa ctacttgaac atgaaattat ggatcacctt tcaaatttag aaaatggaat
      361 tcttatactt attgcgcaac atttattgat tgcaaaatta tgtgacaaaa aaaactagta
      421 gaagaaagaa taatggcttg ataataaagt actacctatt aaaggtagta ctttatttaa
      481 ttta
//
LOCUS       DAZUMB010000328          452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000328 DAZUMB010000000
VERSION     DAZUMB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.357"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>452
                     /locus_tag="ACG80L_003017"
     CDS             <1..>452
                     /locus_tag="ACG80L_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025164.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAZUMB010000329          452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000329 DAZUMB010000000
VERSION     DAZUMB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.358"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>452)
                     /locus_tag="ACG80L_003018"
     CDS             complement(37..>452)
                     /locus_tag="ACG80L_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025165.1"
                     /translation="RKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAY
                     NQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWG
                     NLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          137 a           83 c           75 g          157 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg tt
//
LOCUS       DAZUMB010000330          439 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000330 DAZUMB010000000
VERSION     DAZUMB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.359"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          134 a           87 c           52 g          166 t
ORIGIN      
        1 attattacgt tattaaaaaa acagatgaaa gatcaggttt tattagatct cattctttca
       61 tcacttgaag ggatttaatg aactactttt tcctctttgt cgtttttata tattcgtatt
      121 ccttcaaaca gtaggatagc ctctttcaat gcttgatatt tgaactgtcc gttacttaaa
      181 aatttagatg tgccgccctc ttcctagaac atattctttt ttactttttc attaattgac
      241 agaccaatcg aacgaaactg taaatttcga taggataatg attggtcaaa ctcattcatt
      301 ttcaagacat ctcgattcat attcaagata ggaatctttt tatcaacatc actttattcg
      361 tattttcctc taccataaaa gcttcacgct ccacaaaatt gttttataca ctactattca
      421 gcccattcaa cgaccaatg
//
LOCUS       DAZUMB010000331          427 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000331 DAZUMB010000000
VERSION     DAZUMB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.361"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>427
                     /locus_tag="ACG80L_003019"
     CDS             <1..>427
                     /locus_tag="ACG80L_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5025166.1"
                     /translation="VKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSS
                     ATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAG
                     NKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          174 a           64 c           99 g           90 t
ORIGIN      
        1 cagtgaaaga cggcgtaagc ttaccagaag ataaacttcc agtgttgaaa gcaaaagatg
       61 gttatacaga tgcgaaatgg ccagaggaag caactcaacc aattacagca gatgatacag
      121 aatttgtatc aagtgcaaca aaactggatg ataaatctga cgctgacaaa tataatcctg
      181 aaggtcaaaa agtgactaca gaattgaata aggaaccaga cgcatttgag ggaattaaga
      241 acaaagaaga tttaccaaaa gatactaagt atacttggaa agaaaaagta gatgttagcg
      301 cagctggaaa taaaaaaggg actgttgtag tgacatatcc agatggatca tctgatgaag
      361 ttgaagtaga tgttacagtg acagacaatc gttctgacgc cgataaatat gagccaacag
      421 tagaagg
//
LOCUS       DAZUMB010000332          426 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000332 DAZUMB010000000
VERSION     DAZUMB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.362"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>426
                     /locus_tag="ACG80L_003020"
     CDS             <1..>426
                     /locus_tag="ACG80L_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFS5025167.1"
                     /translation="KSNGFTPTSIVLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMA
                     DNIPIMLENNYFSYERYSFLMEEELTDSLYEVLKVQGTLAINPGETALEIVTADDQIA
                     KIMEVAVGTPFFFMDTLINDQNNIPIHVGNQYYLGEVYR"
BASE COUNT          143 a           73 c           84 g          126 t
ORIGIN      
        1 gtaaatctaa tggttttaca ccgactagca ttgttttgga acgcaagatc attccagcaa
       61 ctgaagggat ggcaaaaaaa ctcgatatcg ctcaaggtga tgatgtttta tatatccaga
      121 gaaagcgaat ggcggataac atccccatta tgttagaaaa caattatttt tcttatgaaa
      181 gatatagttt cttaatggag gaagagctga ctgattcttt gtatgaagta cttaaagtcc
      241 aaggtacctt agccatcaat cctggggaaa ctgcacttga aattgtcaca gctgatgacc
      301 aaattgccaa aataatggaa gtagctgttg ggacgccctt tttctttatg gatactttaa
      361 tcaacgatca aaacaacata ccaattcatg taggtaatca atattacctg ggagaagtat
      421 atcgtt
//
LOCUS       DAZUMB010000333          415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000333 DAZUMB010000000
VERSION     DAZUMB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.364"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>415)
                     /locus_tag="ACG80L_003021"
     CDS             complement(<1..>415)
                     /locus_tag="ACG80L_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5025168.1"
                     /translation="RKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRN
                     EIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKD
                     GQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEI"
BASE COUNT          128 a           74 c           51 g          162 t
ORIGIN      
        1 tatttcttta acgttaaaga tatttactaa acacgctcga ctaattcttg ttaacatagg
       61 atatttcttt tcaagctcac taattcttcc ataaaattcg tactgcccat ctttagtaca
      121 caatgataat ctatgtggta attttgaagc ttctacaaaa taaatgtcat ttatatcaaa
      181 agtaaatgtt tgagaaccta tggaaaaaac aaatgcttga ttcgtaacct gttttgttgc
      241 atctatcctt tcttgcgcta ataacaataa ctcaacaatc tcgtttcgat actcatctag
      301 tgtttgatct tttgttacaa atcctaacgt ttcaactctt ctttttattg ttacgggtaa
      361 catctcatca tgagtagttg taaaaatgat ttttgcttga acatcatatt ttctg
//
LOCUS       DAZUMB010000334          415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000334 DAZUMB010000000
VERSION     DAZUMB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.365"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG80L_003022"
     CDS             complement(<1..339)
                     /locus_tag="ACG80L_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5025169.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZUMB010000335          376 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000335 DAZUMB010000000
VERSION     DAZUMB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.369"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          116 a           59 c           62 g          139 t
ORIGIN      
        1 aaaaacaagg cgagactgat tgatgtctcg ccatttttgt ttccattcta ttgacagctg
       61 ctttcttact ttgtatcttt ttaaaaacgt gtgatatttt ttccgcgagt tttttcaacg
      121 caaggtatat cttgtcataa ttcgcctttc caaaaaaatt caatacataa taagttatat
      181 ccgtttcttt ggggataaga agcaaaaaag gaaagacgta ttattgcttc aaaaactttt
      241 gccattgatt atgttgtaga gacatttggt tatatttgtt caatcatcgg tatcttaacg
      301 gctacgattg tagtaggaaa atctacttat tttatcaagt aagaaaaata gaaaacgcta
      361 atttcttcgt gtctcg
//
LOCUS       DAZUMB010000336          376 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000336 DAZUMB010000000
VERSION     DAZUMB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.370"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..372
                     /locus_tag="ACG80L_003023"
     CDS             <1..372
                     /locus_tag="ACG80L_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5025170.1"
                     /translation="NKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDR
                     ELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNP
                     NMFVNYQNDPYQFTTQVILLF"
BASE COUNT          138 a           49 c           70 g          119 t
ORIGIN      
        1 aataagattt gttgtaaatt cgaatgtcca agtactatta ttgaagttcc tgttccaata
       61 tttgactgta ttagggtgct tggaattatt ctcgataatg caattgaagc atctatagag
      121 agtcaagata gagaactgtc tctagctatt taccgagatg ataaacagat agaaatttcg
      181 atatcgaata gttgtagaga gatactactc tctatagata ctctaatgaa aaaaggaata
      241 acaacaaaaa agggacatca agggttaggt ttatctagta tagaggaaat caataaaaag
      301 aatcccaata tgtttgtgaa ctatcaaaac gatccgtatc aatttactac tcaagttatt
      361 ttgttgtttt gaggga
//
LOCUS       DAZUMB010000337          352 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000337 DAZUMB010000000
VERSION     DAZUMB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.373"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..263)
                     /locus_tag="ACG80L_003024"
     CDS             complement(63..263)
                     /locus_tag="ACG80L_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5025171.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          106 a           66 c           56 g          124 t
ORIGIN      
        1 gaattttttc gtacaaaaaa accgtctttt ctttcgaaaa gacggttaaa agtcaatttt
       61 tattaagctt taacaatgtt tgttgcttga gggccacgtt gaccatcttc aacatcgtaa
      121 gacactgctt ggccttcttc taaagttttg aagccgtcac cttggatagc tgaaaaatgt
      181 gcaaatacat catttccatc ttcaccagtg ataaagccaa aacctttgtc tgcgttgaac
      241 cattttactg taccgttatt cataaataaa ttcctcctga tacgtatcaa tgtacgtttt
      301 ttcgttgcaa taaatatttg ataggcaaaa ggagttttat ctatgtagat at
//
LOCUS       DAZUMB010000338          333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000338 DAZUMB010000000
VERSION     DAZUMB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.377"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..289
                     /locus_tag="ACG80L_003025"
     CDS             86..289
                     /locus_tag="ACG80L_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFS5025172.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          133 a           34 c           71 g           95 t
ORIGIN      
        1 ttactaatga tatttaatat tacaagcaat cttaagaaat tcgaaaagga aaaaatgatt
       61 atgaaaaaga aagaaaaaga aagtattgaa acattgtgtt attctaggaa tattaggaac
      121 ttgtctagct ggcattggta caggaataga cgttgacgca gctacttact atggaaatgg
      181 tctttattgt aacaaagaaa aatgttgggt aaattgggga caatcatggt cagaagggtt
      241 aaaacgttgg ggagataatc tttttggtag ttttagtgga ggacgataat gacttcaata
      301 aacattgatc acacaaaaaa agtcatacat gaa
//
LOCUS       DAZUMB010000339          329 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000339 DAZUMB010000000
VERSION     DAZUMB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.378"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>329
                     /locus_tag="ACG80L_003026"
     CDS             <1..>329
                     /locus_tag="ACG80L_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5025173.1"
                     /translation="YVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIVFT
                     MLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIWGI
                     ISKGWYM"
BASE COUNT           91 a           54 c           72 g          112 t
ORIGIN      
        1 gatatgttgg gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg
       61 tttacggaaa tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt
      121 caattgtttt caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg
      181 tttatgagag tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt
      241 tttcaacaca agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt
      301 ggggtatcat ttccaaggga tggtatatg
//
LOCUS       DAZUMB010000340          323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000340 DAZUMB010000000
VERSION     DAZUMB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.denovo.379"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG80L_003027"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG80L_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS5025174.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZUMB010000341        16367 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.055, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000341 DAZUMB010000000
VERSION     DAZUMB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.055"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..893
                     /locus_tag="ACG80L_003028"
     CDS             <1..893
                     /locus_tag="ACG80L_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5025175.1"
                     /translation="VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLED
                     KDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQ
                     ETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESP
                     EMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVL
                     PNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQ
                     VETNSRVKERLEQIIGSLTG"
     gene            900..1277
                     /locus_tag="ACG80L_003029"
     CDS             900..1277
                     /locus_tag="ACG80L_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFS5025176.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1431..2213
                     /locus_tag="ACG80L_003030"
     CDS             1431..2213
                     /locus_tag="ACG80L_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFS5025177.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2339..3070)
                     /gene="erm(A)"
                     /locus_tag="ACG80L_003031"
     CDS             complement(2339..3070)
                     /gene="erm(A)"
                     /locus_tag="ACG80L_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFS5025178.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(3128..3187)
                     /locus_tag="ACG80L_003032"
     CDS             complement(3128..3187)
                     /locus_tag="ACG80L_003032"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS5025179.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            3579..4241
                     /locus_tag="ACG80L_003033"
     CDS             3579..4241
                     /locus_tag="ACG80L_003033"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5025180.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            4476..4619
                     /locus_tag="ACG80L_003034"
                     /pseudo
     CDS             4476..4619
                     /locus_tag="ACG80L_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            4769..4969
                     /locus_tag="ACG80L_003035"
     CDS             4769..4969
                     /locus_tag="ACG80L_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5025181.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(5223..6902)
                     /locus_tag="ACG80L_003036"
     CDS             complement(5223..6902)
                     /locus_tag="ACG80L_003036"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5025182.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(6904..7116)
                     /locus_tag="ACG80L_003037"
     CDS             complement(6904..7116)
                     /locus_tag="ACG80L_003037"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS5025183.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            7509..9239
                     /gene="efrA"
                     /locus_tag="ACG80L_003038"
     CDS             7509..9239
                     /gene="efrA"
                     /locus_tag="ACG80L_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS5025184.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9236..10996
                     /gene="efrB"
                     /locus_tag="ACG80L_003039"
     CDS             9236..10996
                     /gene="efrB"
                     /locus_tag="ACG80L_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS5025185.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            11091..11288
                     /locus_tag="ACG80L_003040"
     CDS             11091..11288
                     /locus_tag="ACG80L_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025186.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            11441..11926
                     /locus_tag="ACG80L_003041"
     CDS             11441..11926
                     /locus_tag="ACG80L_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS5025187.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(12030..12623)
                     /gene="clpP"
                     /locus_tag="ACG80L_003042"
     CDS             complement(12030..12623)
                     /gene="clpP"
                     /locus_tag="ACG80L_003042"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS5025188.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            12803..13330
                     /locus_tag="ACG80L_003043"
     CDS             12803..13330
                     /locus_tag="ACG80L_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025189.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            13421..14158
                     /locus_tag="ACG80L_003044"
     CDS             13421..14158
                     /locus_tag="ACG80L_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5025190.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            14162..15079
                     /gene="miaA"
                     /locus_tag="ACG80L_003045"
     CDS             14162..15079
                     /gene="miaA"
                     /locus_tag="ACG80L_003045"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS5025191.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            15081..16310
                     /gene="hflX"
                     /locus_tag="ACG80L_003046"
     CDS             15081..16310
                     /gene="hflX"
                     /locus_tag="ACG80L_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS5025192.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5380 a         2712 c         3413 g         4862 t
ORIGIN      
        1 tcgtcctaga gcaattgaac agtcatcttg ataaattacc cgaatatata gctacgatga
       61 ctatgattgt tcaagaatgt ggaatgagga taagtgaatt gtgcaccttg aaaaaaggct
      121 gtctattaga ggacaaagat ggagatttct ttttaaagta ttatcaatgg aaaatgaaaa
      181 aggagcatat agttccaata tctaaagagg tagctttact tattaaagtt cgggaagata
      241 aagtttcaga ggaatttcca gatagtgaat acctctttcc aagaaaagat ggatcgccat
      301 taaaacaaga aacatttaga ggtgagttaa ataaattagc ttatgagcaa aatatagtgg
      361 ataaatcagg tgagatttat agattccatg cccatgcctt tcgccataca gtaggaacaa
      421 gaatgattaa caacgggatg ccccagcata ttgtgcagaa atttttgggg catgaaagcc
      481 cagaaatgac aagcagatac gctcatatct ttgatgaaac tctaaaaaat gaatttacta
      541 aatttcagga aaaactggtt accaataatg gagatgtgct tgatctagat gaagataatg
      601 aagtcgatga tgtagagctt caatggttca agaaaaatat aaatgcacaa gtgcttccaa
      661 atggttattg tagattgcca gtagtagcag gtggttgtcc acatgcgaat gcatgcttag
      721 attgcactca cttctgtacc agtaagcaat tcttaccaca gcacgaagaa cagttagagc
      781 gtacagaaga gttattagcc atagctaagg ataaacaatg gcaaagacaa gtagagacta
      841 atagccgtgt taaagagcgt ttagaacaaa tcattggaag tttgacgggg taattatcaa
      901 tggataaaca agttagaaat acaacagaaa ttgtacgttt ggcgaagcag aaatcaaaaa
      961 agacaaggga aaaagtagac aaagcgattt ctaaattttc gattgaaggt aaagttatta
     1021 attttaattc aatagcaaag gaagctaatg tttctaaatc atggctttat aaggaacacg
     1081 atattaggca aagaatcgaa tcccttcgtg agcgtcaaat aacagcaaat gtagtctcaa
     1141 aacccaagaa aagttctcgt tcggaggaaa tccttattaa aaccttaaaa agaagagtaa
     1201 tggaattaga aaaagaaaat aaaaaattac agaaccaaat tcaaaaatta tatggagatc
     1261 tgtataataa agaataatta ttaatctgta gacaaattgt gaaaggatgt acttaaacgc
     1321 taacggtcag ctttattgaa cagtcattta ggtatatgtc caatctaggg taagtaaatt
     1381 gagtatcaat ataaacttta tatgaacata atcagttacg aggtgaaatc atgagcaatt
     1441 tgattaacgg aaaaatacca aatcaagcga ttcaaacatt aaaaatcgta aaagatttat
     1501 ttggaagttc aatagttgga gtatatctat ttggttcagc agtaaatggt ggtttacgca
     1561 ttaacagcga tgtagatgtt ctagtcgtcg tgaatcatag tttacctcaa ttaactcgaa
     1621 aaaaactaac agaaagacta atgactatat caggaaagat tggaaatacg gattctgtta
     1681 gaccacttga agttacggtt ataaatagga gtgaagttgt cccttggcaa tatcctccaa
     1741 aaagagaatt tatatacggt gagtggctca ggggtgaatt tgagaatgga caaattcagg
     1801 aaccaagcta tgatcctgat ttggctattg ttttagcaca agcaagaaag aatagtattt
     1861 ctctatttgg tcctgattct tcaagtatac ttgtctccgt acctttgaca gatattcgaa
     1921 gagcaattaa ggattctttg ccagaactaa ttgaggggat aaaaggtgat gagcgtaatg
     1981 taattttaac cctagctcga atgtggcaaa cagtgactac tggtgaaatt acctcgaaag
     2041 atgtcgctgc agaatgggct atacctcttt tacctaaaga gcatgtaact ttactggata
     2101 tagctagaaa aggctatcgg ggagagtgtg atgataagtg ggaaggacta tattcaaagg
     2161 tgaaagcact cgttaagtat atgaaaaatt ctatagaaac ttctctcaat taggctaatt
     2221 ttattgcaat aacaggtgct tacttttaaa actactgatt tattgataaa tattgaacaa
     2281 tttttgggaa gaataaagcg tcctcttgtg aaattagaga acgctttatt actttaattt
     2341 agtgaaacaa tttgtaacta ttgaaaatag aaagaaattg ttccttcgat agtttattaa
     2401 tattagtgac atttgcatgc ttcaaagcct gtcggaattg gtttttagtg aaaagaacac
     2461 gatattcacg gtttacccac ttataaacaa aagatcgata ctttttgtag tccttctttg
     2521 aaatcaatgg ttgatgtcgt tcaagaacaa tcaatacaga gtctacactt ggcttaggat
     2581 gaaaatatag tggtggtact tttttgagca tttttatatc catctccacc attaatagta
     2641 aacccaaagc tcgttgcaga ttttgcaatc ttttcgcaaa tcccttctca acgataagat
     2701 agctatattt agcctgactt tcaaaggtaa ttcttttgac aatatccgta ctgatgttat
     2761 aaggaatatt accatatatc ttatagttta tatgttttgg gaaggaaaat tttagaatat
     2821 ccgtttgaat cacttttata ttctcagagg ggtttaccgc ttctttagtc acttgacata
     2881 agcctccatc aatttctata gcagtaactg atcgactcat tttgactagc tctttggtaa
     2941 aatgtccttt tcctgatccg atttctatta cgttgtcttg tttactgata ttcgtgtgat
     3001 tcaatatttc ttttacatgc tttttagaag taataaaatt ttgcgtgtct ttagggtttt
     3061 tctggttcat tataaccttc tccttactgg ttataatgaa ctgacattaa gcgttcatta
     3121 taaccaatta attttgattt ggttgataac gaactttatt aataacaaat atagaaaaag
     3181 tacccatact tttatctcct ttccattttt ttcattatga atgagataaa gtaatttcta
     3241 ctactgcgat actggtgcac ataattgaaa cctccttatg taactgaata tgattgtatc
     3301 atcttttcat tttagaataa acctttatat gtaattttgc acttgacact tcagatggac
     3361 gttttcttca aaataatatg ttttgaaact tgataagaga agccactgtc tttactaaaa
     3421 agtaaaggct gcgataaagg acagtgactt taataaagtt gcactgttgt attgtctttg
     3481 gataaaattg tctaaaggga agttttatcc ccaacgaaaa aacatctaaa gtggtaagta
     3541 atgaaagaat gtcgataaag taggaatggg ggatcttttt gaatacaata gaatcacagt
     3601 ttgataaggt tgcagaagat tacgatttcg tgaatgagct tttgaatgat tattcattct
     3661 ttgtgtctaa tatgtctcca aagaaaggca gagcattaga tatcggatgt ggctcgggtt
     3721 tgttagtgga gaaattagca agttattatg atgaagtggt agggattgat atttctaatc
     3781 aaatgctcga tcttgccaaa tctaaacgtc aactaacaaa tacggtctat ctgaatatga
     3841 atgcagaaca acttaatttt aatgagaagt ttgattttat tgtaagccga acaacctttc
     3901 atcatttgga tgatatagcc agcgtaatac agcaaatgaa ggaactgttg aatgaggaag
     3961 gaagaatagt tattcttgat aatgtatctg aagtcgagac accgcctacc tatgtttata
     4021 aattaggggc aatccaagag tttttaccgc attgtttcaa atttggaata aaaaatgcga
     4081 ttagaattta taatcacaac acatctaaat catggcttga gcatttagct tcagataagt
     4141 acctatctga acaaaattat tacgatttat atgaaaagtt gttgcctgga tgccaattcc
     4201 ataaaatggg ttgggcaatg ggggttgtct ggacaaaata acttaaagct gtggctttga
     4261 aaagttgaat ttgtgattgt tttcagagct atcgggtgct ttagtataaa taacaatgaa
     4321 taaaaaatca gcctgtatct tatgttacta ttaagtaatt taagatacag gttttactat
     4381 ttttgaaata agtaccttcc acttcaaata catgtattca aagtgaaagt attgatttaa
     4441 atgattcttt tgtaatttac tcttgataat atgaaattcc atcgcagcag gatatgaatt
     4501 ttaccaaaag gatacaaaag tgtggagaaa tgatggggat cactgtactg gaccacttga
     4561 tcatcggacg caaacgatat tttagtttga gagaagaagg aatgatggaa gagaagtaag
     4621 ttgctttctt gcaaaaaaaa tgaagcaagt gtacaattat attgtaattt tgtttgggaa
     4681 tagaagttga aacatctgtt gcacctctat cgtacacgag caatgttaca atttaaatta
     4741 gtgcttttgg cacgaggagg atattattat ggaaaacgga acagtaaaat ggttcaacgc
     4801 tgaaaaagga tttggattta tctcacgtga agatggaagc gacgtattcg ttcacttctc
     4861 agctatccaa ggtgacggat tcaaaacatt agaagaaggt caagcagtga ctttcgacgt
     4921 tgaagaatca gaccgcggcc cacaagctgc aaacgttgta aaagcttaat tttatctgta
     4981 aatcaaattg atggtagaca tcaattttta cgagagaaca aagcgagtaa caaaaatacc
     5041 tgccacaaga gacaatttct cttgtggcag gtattttttt gtatgaaaaa gtaaataaat
     5101 ttaagaggct atgaaagagc caccttgtat caaggaataa gaacaaacaa aaatggcttc
     5161 tcatgttttt catttgtttt ctcttatatt caggacaagg tgctgatttc ataacctctt
     5221 atttattctt catctggtgt cattaccatt ggcaaaatca ttggatgtct ttctgtacgt
     5281 tcgaataaga aaggctgaag cgcgccagtc atcgcttctt tcaatttata ttctgaacag
     5341 tcaggtccat tcaaacattc tcttaaagcg tggaataaga ttcgctgagc ttcattgatc
     5401 atttctcctg attctcgcat gtagacaaat ccacgagaga gaatatctgg accagccaag
     5461 atttcttttt tcttgatatc cactgtcgcg acagctaata caagaccttc ttctgaaagc
     5521 aggtgacggt cacgtaaaac gacattacca atatcaccga tcccgctacc atcgatatac
     5581 acatctccag ccacaaaatg cccagcagga cgcgcagaat cgctagttaa cgctaaaaca
     5641 tcgccatttt ccatgatgaa gcagttttct gcaggaacac ctacatcttg tgctagagaa
     5701 gcatgtactt tcaacatgcg gaattctccg tggacaggaa taaaatattt tggcttcatc
     5761 aaacgaagca ttaatttttg ttcttcttgt ccaccgtgac cggaagtatg gatgttattg
     5821 atttttccgt gaatcacttc tgcaccagct tcagacagca aattgatcaa atgattgaca
     5881 cttgttgtat taccaggaat aggtgaactt gaaaagatca ctgtatcgtc tggctggatt
     5941 tggatttgac ggtgagtacc attcgcgatg cggctcaacg cggccatcgc ttctccttga
     6001 gaacctgtac ataggatcat cgtttcattg gcaggcaagc gattgatttc gtgtgcatct
     6061 acaaatgtgc cgtctggaac tttgatatat cctaaacgtt gaccgttgac gatggcattt
     6121 tccatgcttc gtccgaaaac agcgatttta cgaccagttg ccacagcggc atctgctgct
     6181 tgctgcaaac ggaagatgtt agaagcaaag ctggcaaaaa tgatacgtcc ttcgattttt
     6241 gaaaagatct tcaaaataga atgcccaatc gttttttctg atttagtaaa tgttggaatc
     6301 tcagcatttg tactatcaga taaaaggcac aagactcctt cttccccgat acgagccatt
     6361 ttatgcaagt ttgctggttc gccaacagga gtgaaatcga atttgaagtc tccagtcgct
     6421 acgatatttc cagaaggtgt tttcaccacg atccctaatg catctgggat actatgggtc
     6481 gttctgaaaa agctgacaga agtctttcta aaacggatga tcgtatcttc attgatctca
     6541 tggagctctg catctcgaag caagccatgc tcatctaatt tgtttgtgat caacgctaat
     6601 gcgagaggac cagcatagat aggaatattt gcttgtttca gcaagtaggg tattccgccg
     6661 atgtggtctt cgtgcccgtg agtaatcacc aaggctttta ctttgtgtaa gttttgtatg
     6721 atgtagctgt agtcgggaat gacatagtca attccaagta aatcgtcttc cgggaatttg
     6781 atcccggcat caatcaaaat gatttcatct tggaattgta ccccatacgt atttttcccg
     6841 atttcaccta atccaccaat agcaaagacg cctgtctcgt tatttttgat ggaaagtttc
     6901 atattaaaac tccactaatg agaaatcagc attttctttt tcgtactcta gatgacttcc
     6961 ttctagaagt tgaacgaatt caatattgta tggggtattt tcttccacac gttggcgagc
     7021 gtctacatcg ctttttgcct caatgtaaag agattttgtt tcttcccgtt tagggttgcg
     7081 tacttttgtt tcttgataat aaactttata gatcattgtt ttgtctcctt tatctgcgta
     7141 aagcagtgat ttagctagtt gattttttta gacaacacaa aacgagggga caagccacct
     7201 ggtttttcat atgctttgac tagaatactg gattcgagtc aaagagacaa gcctcgtttt
     7261 tgcttatctg ttcggatatt ttttcaaccg taccttctgt ataaaaaata tacaacaatt
     7321 aattgttatt ctagcataaa acagcaaata agtatagaaa tcaactagat tgaaagaaaa
     7381 aatcaatgtt tatcatcccg ctataatcat aatgactttt ttatgagaga agtgttctat
     7441 taataggctc gttgtgctat catttttagt aatgaaaatg taacaaaaga ttaaaaggag
     7501 atgagaaagt gaaactaatg tggcattata ccatgcgtta taagaaattc ttgtttttca
     7561 atttcatatg cgtatttggt tttattttga ttgaattagg acttcctacg attttagcac
     7621 gtatgatcga tgtaggaatc cgcaatgatg attttgatta tgtgaagcaa caagggatat
     7681 ttatgatcgt tattactgta atcggtatta ttttgaatat cttgttaggt tattttggtt
     7741 cgaggattac gactaatatc gtagcagata ttcgagatga tttgttcgaa aagatccaaa
     7801 gttattcgca tcaggaatac gaaaatctag gcgtatcttc attgatcact cgtacaacaa
     7861 acgatgctta tcagatcatg ttgttcctgc aaaatatttt acgtatcggt tttatggcac
     7921 caatgatgtt cattgtcagt ttgtacatgg tcatgcgtac aagtgtgaca ctcagtttgt
     7981 atgtagtagg tgcattacct ttactattat tggcagtcgt agcgattgct aaggtgtctg
     8041 aaccgttatc taaaaagcaa caaaaaaact tagataaaat caacagtatc ttgcgggaaa
     8101 atctttcagg attgcgtgtg attcgtgcat tcgtcaatga aaagtttgaa gaaaaacgtt
     8161 ttgctgtagt caatgagaat tatgcttcta gttcaaaaag cttattccgt ttgatggctg
     8221 ttgcacaacc gggtttttat ttcttattta atatcgtaat ggtattgatc atctggagcg
     8281 gggcagtgca aatcgatgca ggcgagcttc gtgtaggaga tttgattgcg tttatcgagt
     8341 atatattcca tgccttgttc tcattcatgt tatttgctag tgtgttcatg atgtatccaa
     8401 gagcagctgt ttcagctgga cgtatccaag aagccctaga tgccaaacct tcgattgtag
     8461 aagatccaaa tggtatatct gatacaaaaa ctaaagggta tatcgaattt caaaatgtaa
     8521 catttgctta tccaggccat tcgcaagagc cagtgatacg aaatgtcagt tttacagcct
     8581 ctccaggaga gaccgttgct tttattggaa gtaccggtag cggaaaatct acgttgatcc
     8641 aattgatccc tcgcttttat gatgtgagtg aaggaaagat cttgatcgat ggagtagacg
     8701 tgagagatta tcggctaagc aagttgcgag ataagattgg gtttattccg caaaaagcgc
     8761 tgctatttac tggaacgatt gcagataatt tgcgatacgg gaaagaagat gctacgcaag
     8821 aagaaatgga acgtgcagct gagattgctc aagcttcaga attcatttct cgaaaaccag
     8881 atggatttaa tgaacattta tcagaaggtg gttccaattt ttcaggtgga caaaaacaac
     8941 gattggcaat agccagagcc atcatacgcc gtccggaaat ctatattttt gatgacagct
     9001 tttctgcatt agattatcaa acggatgcga agttacgtgc tcgtctgaaa aaagaaacga
     9061 cagaatctac tgtattgatc gtagcacaac gtgtgggaac gatcatgcat gcagataaga
     9121 tcatcgtatt aaatgaagga gaagtcgtcg gtatggggac gcatcgggag ttgttggaaa
     9181 aatgcccgat ttattatgat attgccgctt ctcaattatc aaaggaggaa ttagcatgaa
     9241 aaaaaccttt tcttccttaa aacgtttagc tcgatatatc agaccgtatc gtgagacttt
     9301 tcttttagtg atcctattta cagttctgac agtggctttc aacgtggctt tgccatatgt
     9361 tacaggactt cctactacag aaatcagccg taatattgcc aatggcgaat caatcaattt
     9421 ttcttatata ttcaaatgtc tcatttggat cacagttgtt ggaatagggt attgtatttc
     9481 gcaattactt tctggtgttt tgatgacaaa tgtcgttcaa agtgccatgc atgatttgag
     9541 aagagacata gaagaaaaaa tcaatcgtct tccggtttca tatttcgata aaaaccagca
     9601 aggaaatatt ttgtctcgtg tgacgaatga tgtagatgca gtcagcggtg ctttacaaca
     9661 agcttttatt ggtatcgtaa atgcgatatt gggaattacg ttggctgcag caatgatgtt
     9721 ttatatccag ccgatgatgg cgctgatctc tatgatcatg attccaacat cgatctggat
     9781 ctcaaaacga gtgatcaatg cttcgcagaa atattttcaa agcatgcaga attctcttgg
     9841 tgagctaaac ggctacgtcc aagaaaatat gacaggtttt agtgtattga aagtatatgg
     9901 acgcgaaaaa gaaacattgg aaggattcaa aaaagttaat cacagcttga aatactttgg
     9961 gtttcgtgca gcattcattt ctggcttgat gatgcctcta gttcaattaa ctgcctatgc
    10021 tacttatatt ggtatggctg ttctaggaag cttttatgtt attactgggg cgatcgttgt
    10081 cggtcaacta caagcattca tccaatatat ttggcaaatc agtcagccaa tggggaacgt
    10141 gacacaattg tcttcagttt tgcaaagtgc atcagcggca acgaaacgtg tattcgagat
    10201 tttagatgag ccagaagaaa aagaaaacaa agtagatgct tcactgccag aaacgatttt
    10261 aggagatgtg acattcgatc atgtcgattt tgcatatgat ccgaagaaac cattaatcca
    10321 agatttgaat tttgaagtga aagctggtca aaccgtagct gtggtcggtc cgaccggtgc
    10381 cggcaagacg acattgatca atctattgat gcgtttttat gacgtagaca aaggagcaat
    10441 aaggattgat ggaatcgaca ccaaatcaat gagtcgcagt gatgtgcgtt cgctatttgg
    10501 tatggtcttg caagatgcat ggctttatca aggaacgatt gcagataata tccgcttcgg
    10561 aaagcttgat gcgacagatt atgaagtagt tgatgctgcg aaaacagcaa acgtagatca
    10621 ttttatccga acgatgccag atggatatga tatggccatc aattctgagg gagacaatgt
    10681 gtctttagga caaaaacagt tgctgacgat tgcccgagca gtggtctccg atcctaaaat
    10741 cttgatttta gatgaagcaa caagttcagt agacacccgt ttagaagcgt tgattcaaaa
    10801 agcaatggat cgcgtgatgg agggacggac aagtttcgtc atcgctcacc gtttatcgac
    10861 aatccgtgat gcagatctga ttctagtgat ggatcaaggt cagatcattg aaaaaggaac
    10921 gcatgagagt ttattggcac aaggtggatt ttatgaaaaa ctgtataaca gccaatttgc
    10981 tgaggaagca gaataagtca tataagaata aaacagttta aaacttattt tttgttctct
    11041 attcttgata atggtaaagt aaagttatca agaaaagtga ggacacggag atggaacagt
    11101 ttttaaatga aaaaattact gaagccaaaa gagaagttga tcgtttaacg cacgtctgtc
    11161 agacgaagcc ttttgattca gctgattttt ttgaaaatga gtttcaaatc gaacgcttgg
    11221 cagcacaaat agaagtgtac gaattaattt taaaaaaagt aatagaagaa aatatagatg
    11281 tacaataaaa acaggtaaaa cgtcagttac cagatactgt aaatagacat ctggcctgag
    11341 caggttttac ctgttttttt tcttatgaaa taaatatatg ctatgatacc tttattcaat
    11401 aggattagtt tctacaaaaa atgaaaggag aaaaataaat atggaacggt ttatagcatc
    11461 aaatgccaca gtgattggtg atgtgacatt gtcagaagat gtgacgatct ggtatcaagc
    11521 tgttttacgc ggggacagta attggataaa aataggacaa agaacgaata tccaagatgg
    11581 tacaatcatc catgtcgatc atgatgcacc tgtagatatt gcggagaatg tgacggtagg
    11641 acatcagtgc atgctacatg gctgtacaat cgaaaaaggg gcgctgatcg ggatggggac
    11701 cacgatattg aatcatgcgg ttattggtga gaatagtttg attggtgctg gatcattagt
    11761 aacagaagga aaagttattc ctcctaatgt cttggctttt ggtcgcccag ctaaagtgat
    11821 ccgtcctttg acaaaagaag aaatacaaaa aaacaaagaa aacatccagc attatgtcga
    11881 gataggtcgg atgcatgcgc aaggaaaatt caaggagcaa agataggcag tagcatccat
    11941 aaaataaaaa aggatggtca gtttgagttg accatccttt tttatttggt ttagataaga
    12001 gagcttaaaa aagcttcctt tgaaacagat taatttaatg aagaactgtt ttccatgact
    12061 tcatcgatca atccatatgc ttttgcttgt tcagctgtca tatagttgtc acgatctgta
    12121 tctttttcaa tcacttcaag cggttgtcca gtgcgttcag ctaaaatttt gtttaagcgt
    12181 tcgcgtgttt gcaaaatatg gcgtgccgca atttcgattt cagttgcttg cccttgagcg
    12241 ccgccaagag gttgatggat catgatctca gcatttggta aagcaaatcg tttacctttt
    12301 gtaccagctg tcagcaagaa gcttcccatt gaagcagcca tacccataac aattgtttgg
    12361 acatctgctt tgacaaagtt cattgtgtcg taaatagcca tacctgctgt aactgaacca
    12421 ccaggagaat tgatataaag atagatatct ttttcagaat cttgagcgtc taagaaaagc
    12481 aattgggcga tgatcgaatt agcaagatca tctgttactt ggccgcttaa catgatgata
    12541 cggtctttca ataaacgtga gtagatatcg taagctcttt cacctcgtga agattgttca
    12601 atgactgtag gaattaaatt cataatagag ttcctccttg atttcgttta gaaatagtgt
    12661 accataattt agtttctaat acctaaaatg tagtatacat ttttggtcaa aaaaggtcaa
    12721 ataaaaaact taggcaaatt agtcgcataa acccgcttta tggtatgata agcagagaaa
    12781 caaaacaagg aggaacaaca gattgatagt tggtattact ggccttatcg gagtgctgac
    12841 aggtgctgta gttgtctata tcggcatgat actgcaaatg cgtcacttgg aaaataaaga
    12901 acagcgtaga cgtgaattgg aattgaaagt caaagaaatc gagacgctga atcaattaaa
    12961 taaaaagatc aacgagatct tgcaaaaacg gacaacgctg atggaagaat atgtcagctt
    13021 tgatgcattt gatgattgtt atatcacgat tgacgatttt gcgtatctcc aatcattttc
    13081 agcgcaaaat agtttttatc tgccaaatta tattttagac gagctattta agaatatctc
    13141 ccatcgaaaa gtgattttat ctccagatga aacaatgaag attggtggat atgcttataa
    13201 aggcggacgc gtgattttag agaattttac ggatagtctg actgaaatga tcaatgaaaa
    13261 aaaagctcag ttgaagacta tgacgcgcca gccgattggt ttcttctcga aagatgatgt
    13321 ttggagctga tattttgaag cattgccgtt gcacatgaga gcgtggtaat gctttctttt
    13381 tttcttaatg atacaataga actgaacgga gtgaagaaag atgttacaaa tctatgccca
    13441 tcgcggaagc agcggcaccc acccagaaaa tacgcttcct gcatttgcag aagctgtacg
    13501 agtaggtgca gatggtatcg aactagatgt ccatctaagc aaagatggtt atttgatcgt
    13561 tatgcacgac gaagaagtgg accggacgac taacggtaaa gggctgattc gagaaaaaac
    13621 attggaagaa ctcaaaaaat tgaatgcagg gggcaaattc agcaaagaat ttccagcagc
    13681 taaagttcct gaattgagag aagtgttagg actgctcctc caaaaaaatt accgagggat
    13741 gctaatgatt gaaattaaaa ctgatcagta tgaatatccc ggtatcgaag aaaaattagc
    13801 cgaaacatta aaaagtagag attggccttt tcaacatgca tattgcagct ttcatttgga
    13861 ttctttggaa aaaatcgcat tactggaacc ggagatacag ctggacttat tgatgagtac
    13921 ttctgataaa aagatccaac tagcagcaga aaccccttat atagaaggag tacatccaaa
    13981 aaatacttgg ttgttcgaac atccagtcca ggcagctgat tttccaaaag caattcgtgc
    14041 atggacgatt aacgatgaag aagaaatggc caaatgtcaa gaactcaaaa tagatggaat
    14101 aataactgat tttccggaga aagctcttcg gtggaaacaa tcaacgaaaa gtaggtaaaa
    14161 tatggaaaaa gtattagtga tcgtcggtcc gacagcagtc ggtaaaacag cgctaagtgt
    14221 ggaactcgca caactatttg gaggagagat catcagtggt gattcattgc aaatctataa
    14281 aaatctggat atcggtacag caaaaatcaa agaagaagag atgaataatg ttccgcatca
    14341 tttgattgat gtgatcgaac ctactcaacg ttactcagcc gctgattttc agcattcagg
    14401 tcgacaattg attaccgaga ttgcgaaccg agggcaccta cctatcatag ctggtggtac
    14461 gggtctctat atccaatccc ttctatatga ttatcagtta ggagcgacag aagaagcgaa
    14521 tcctgagatt agaaaaaagt atgaaacgtt agctgaaaca attggttccc aagcattatg
    14581 gaatcagttg aaagaaaaag acccggcagc tgctgagaaa atccactgga acaatcaacg
    14641 caaagtgata agagctctgg aagttttaga gacgacagga catagtatca cggcacctaa
    14701 agaagagcca aagcaattgt atgattacct catgattgga ttagatacga agcgtagtat
    14761 tctttatgaa cgaatcaatc aaagagtcga tttgatgcta gcagaaggtt tagtagcaga
    14821 agcgcgtaag gtgtatgagc ttggagaagt ccaggccagt caaggtattg gatataaaga
    14881 atttttccct tattttgaag ggaaagaaac attggaagaa gcaacagaac aagtcaaact
    14941 acattcgaga agatacgcga aacgtcagtt gacgtggttt cgtaaccgtt tggatgcaca
    15001 gtggtttgat cttataagtg agccaaatca aaaaagtcag attgagcaaa caatcaaaaa
    15061 atggctggag gcggaataaa atggaagaaa aagtgatcat cgtaggggta gaaaccgaag
    15121 caaaccagcg ttacttttca gagtcaatgg aagagctagt tcagctgacg aataccgcat
    15181 caggagaagt cgtattcaca attacacaaa agagaccaca agtggatcgg caaacgatta
    15241 tcggtaaagg gaaattacaa gaattagttc aacaagcgga tgcgcatgaa gcggatttga
    15301 tcatctttaa tcatgagcta actccgagac agagccaact gattaccgat gctgttggtc
    15361 ttccggtaat tgatcgcgtg cagcttatct tagatatttt tgcgatgcgt gctcgttcga
    15421 aagaagggaa gctgcaggtt gaacttgctc agttagaata cctgctgcct cgtttagtag
    15481 ggcaagggaa aacattatct cgtcttggcg gtgggatcgg aacaagaggg ccaggtgaaa
    15541 caaagttaga aacagaccgc cgacatatac ggaataaaat ttttgctgtt aagaaagaat
    15601 tgaaagaagt agaagctcat cgagagcgca atcgccaaaa aagaaaaaat agcgagattt
    15661 ttcagattgg acttattggc tacacgaatg ccgggaagtc aactatcttg aatctattga
    15721 cacaagcgga tacttattcg aaagatcaat tgtttgcaac gttagatcca ttgactaaga
    15781 gatggcgttt tgctgaagga tttgagatta ctgtaacgga taccgtcggg ttcatccaag
    15841 acctgcctac gcaactgatc gatgctttcc attctacatt agaagaaagt caaaatatgg
    15901 atcttttgct acatgtagtc gatgctagtt ctccagatcg tattttacag gagcagactg
    15961 tcttaaaatt gatggatgaa ctgaatatga aagaaatgcc gatactgact gtttacaata
    16021 aggcagacca gatcgaccca gcgatgttca cgcctacgct attcccaaat gtattgattt
    16081 ctactcagac gaaagaaggt aagatggcgt tgatcgaagc aatcaaacgt cagctgatgg
    16141 aactaatgac cccttataca ctttctgtgc caagcaacga aggacagaaa ttaagcgagt
    16201 tgagaagaca aacaatggtt ttggacgaag cgtacatgga agaaacaaac gaatatgaag
    16261 ttaaagggtt tgcaaaaacc aattcaaaat ggttaagaaa aaaccaataa aattttattt
    16321 ttttaacaat attatttgtc aaattaagcg agacaaaact ttccatt
//
LOCUS       DAZUMB010000342        12703 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.080, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000342 DAZUMB010000000
VERSION     DAZUMB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.080"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG80L_003047"
     CDS             complement(134..577)
                     /locus_tag="ACG80L_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS5025193.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG80L_003048"
     CDS             complement(795..2135)
                     /locus_tag="ACG80L_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5025194.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG80L_003049"
     CDS             2404..3300
                     /locus_tag="ACG80L_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5025195.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG80L_003050"
     CDS             complement(3450..4391)
                     /locus_tag="ACG80L_003050"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5025196.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG80L_003051"
     CDS             4517..5638
                     /locus_tag="ACG80L_003051"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5025197.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG80L_003052"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG80L_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5025198.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG80L_003053"
     CDS             7465..7992
                     /locus_tag="ACG80L_003053"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5025199.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACG80L_003054"
     CDS             8203..9264
                     /locus_tag="ACG80L_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5025200.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACG80L_003055"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACG80L_003055"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5025201.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACG80L_003056"
     CDS             10177..12159
                     /locus_tag="ACG80L_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5025202.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG80L_003057"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG80L_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5025203.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         4186 a         2327 c         2632 g         3558 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tag
//
LOCUS       DAZUMB010000343         8646 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.110, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000343 DAZUMB010000000
VERSION     DAZUMB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.110"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..1294
                     /locus_tag="ACG80L_003058"
                     /pseudo
     CDS             167..1294
                     /locus_tag="ACG80L_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            1392..1580
                     /locus_tag="ACG80L_003059"
     CDS             1392..1580
                     /locus_tag="ACG80L_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS5025204.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1638..1991)
                     /locus_tag="ACG80L_003060"
     CDS             complement(1638..1991)
                     /locus_tag="ACG80L_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5025205.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2522..2944
                     /locus_tag="ACG80L_003061"
     CDS             2522..2944
                     /locus_tag="ACG80L_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5025206.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2941..3171
                     /locus_tag="ACG80L_003062"
     CDS             2941..3171
                     /locus_tag="ACG80L_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5025207.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3669..3869
                     /locus_tag="ACG80L_003063"
     CDS             3669..3869
                     /locus_tag="ACG80L_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS5025208.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3896..5089
                     /locus_tag="ACG80L_003064"
     CDS             3896..5089
                     /locus_tag="ACG80L_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5025209.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5157..6722)
                     /gene="guaA"
                     /locus_tag="ACG80L_003065"
     CDS             complement(5157..6722)
                     /gene="guaA"
                     /locus_tag="ACG80L_003065"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5025210.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6781..7704)
                     /gene="coaA"
                     /locus_tag="ACG80L_003066"
     CDS             complement(6781..7704)
                     /gene="coaA"
                     /locus_tag="ACG80L_003066"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS5025211.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7900..8505
                     /locus_tag="ACG80L_003067"
     CDS             7900..8505
                     /locus_tag="ACG80L_003067"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5025212.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
BASE COUNT         2828 a         1550 c         1627 g         2641 t
ORIGIN      
        1 ttatattatc ataataccac aaaatcacaa attttcattc aattatcatt ttcaattgca
       61 ttttataaat cattttgtat gtaatttggt atgtaagtta aaatgaaaaa agtagtccat
      121 tttgaagtac attttgtcat tttgtactcc aaaactgaac tacttacttg catatatacg
      181 cctttatagt ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat
      241 aaaagttaca gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta
      301 ttctggagaa attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga
      361 tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac
      421 tgttgaaccg agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc
      481 agatagtgat ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc
      541 tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt
      601 ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga
      661 atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt
      721 atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt
      781 aaatcaatca tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt
      841 atatggttgg aatgtgacgg attgtaaaat ctgttttaag tacggtttat actatagccc
      901 tgttagtact ccagcagatt ttcggatgct tactcctatt gtactggagc aagcctttag
      961 aaaagctgga acagaattgt tagagccata tcttagtttt aaagtttatg caccacagga
     1021 atatctttca cgggcatata acgatgctcc caaatattgt gcaaatatcg taaatactca
     1081 actgaaaaat aatgaggtca ttattattgg agaaattcct gctcgatgta ttcaagatta
     1141 tcgcaatgat ttaacttttt ttacaaatgg gcttagtgtt tgtttagcag agctaaaagg
     1201 atatcaggtt accactggcg aacctgtttg ccagacccgt cgtctaaata gtcggataga
     1261 taaagtaaga tatatgttca ataaaataac ttagtgcgtt ttatgttgtt atataaatat
     1321 ggtttcttat taaataagat gaaatattct ttaatataga tttgaattaa agtggaaagg
     1381 aggagattgt tattataaac tacaagtgga tattgtgtcc tatttgtgga aataaaacaa
     1441 gactacgaat acgagtggat actatactta aaaatttccc tttatactgc cccaaatgta
     1501 agaacgaaac tttaattaat gttcaaaaaa tgaatataat aacaatcaaa gagccagacg
     1561 ccaagacgca gagccgataa tttgagaaat gaaactctca tcttatcggc tctttttgtt
     1621 tatctgaatt ttactgacta gccttcaata tttcttgctt cattgatacc ttttgctagt
     1681 gcttccatta aggatagttc tttgtctgta aagctatcca tgtatttttc tacctgtaac
     1741 cttcgagtgc ttttaatcaa attatctgta ggtaagaaaa attcatcaat agatacatgg
     1801 agtaatgata caagatcata cagcacttgt gtacttgggt gttgtccttt gttttctata
     1861 tttgttaaat aacgtgggtc aatttcaatc attgttccca cttgttcacg agtgagaccc
     1921 cgtttcattc gagcctcttt aatcgctaaa ccaacggctc taaaatcgta tttatcttct
     1981 tttttacgca tactaaatca cctctataca ttttactgtt cctatataac cataaacagg
     2041 tatagaaaaa catgttatat agtttatgag ttcctataga acactgtgga atttattaat
     2101 gcgatgaatc caatttcaaa tagaaaggtc gctgatttta tttacctata caactttata
     2161 aaatttagtc atactcctag aaagtagata aaattggcga cttaatggag ggaaaagtcg
     2221 ccatgagaaa gataatctgt catctagttc tcttaattaa gttcttatca aaaaaactta
     2281 tccacctgta ctggatagat atgaccatca atgtggtttt gcagattaca tagatagaaa
     2341 aatcaaagca tatgaacaga gaaaacaatc tgtttatgtg cttttttggt cacttaataa
     2401 atatctttta ggatttctct tcatttatgc aacaaatccc ccgttcgtgt cggggtttag
     2461 tatgaaggga gataaagaag cacatgccac tccctttcac gacagaaagg gggtgaaaag
     2521 catgaaacca tcttcttttc aagaaaccat agaaaatcag tttgactata tctgcaaaca
     2581 agttattgaa gatgaacgaa aagattactt caaacatcta gacagactat cagcaaaaga
     2641 agtctcgttt tctgatatag ataactacct tgtcaactct ttttctactg ttgatacgta
     2701 cgtcactgat ttccaactat ttaaattatt tggattaaga atcaatgttg aaagtgattt
     2761 attaagtgaa gccctgcgtc atctttcaga gaaaaaacgg aacattattt tacttcatta
     2821 ctttatggat atgagtgatg tggagattgc ggagttacta aatttgaatc gttcgactgt
     2881 ttatcgtcat cgaataagtg gactagccat gattaaagaa ttcatgaagg agtgtaaaga
     2941 atgaaaacag cttatccaag ggttcccttc cccctcattg taaaggcgac agatggcgac
     3001 gtggaagcaa ttaatcagat tgttaaacat tatcgagggt atacctcaaa acgttcatta
     3061 cgtcgcatga cagacgaata tggaaattct catatggtta ttgatgaaac cctacgtggc
     3121 agaatggaaa cgaaactcat ctctaagatt ttatcgttcg aaattaaata gtcccatttt
     3181 ttcgtggaag cgtgtcgaat cgtatccacg cttcccgatt tgggaggttt gttattccat
     3241 caaagcatat tgtgtgttca gtaggttttg ataggctaac aagccaaacg ttctttgaca
     3301 accaaataaa ggcaatcgaa tacgtttcgg taagaaaaga gctgatgaac taacacgcca
     3361 tgacctacaa aattagtgag cgattcatgt tagtaattcg aaagtaatct ttagcaggat
     3421 tgctgccatg actttcttat cgtgataatg atactcccgt atagccatag tccgagcgtt
     3481 agaagcgtca caggcaatgg gtacggctac atgagaacca tgcagaagtg aaattcctgt
     3541 gagctttgct agagctgttc gattgctaag atattcttct cttgtcagtg tattattata
     3601 aaactatttg atataattat aggtgaatat tggagttttc ctagcctata attgggaaag
     3661 gagtaataat gataacggtc acaaaaaagg atttagttga gctaggatat ggaacttctt
     3721 tttcagcaga catcataaaa aaagcaaaag aattgatgat tacaaaaggg catatctact
     3781 atcaatctcg aaagctagat agagtccctc gtgaagctgt tgaagaaatt ctaggaattc
     3841 ggttttcaaa tgaaaacagg tctgagtagt ttttgcacag taaggagtaa aaactatggt
     3901 taaagaccca ataaaaaaag caaagaatgg aacctattat tttcgtgcaa atctaggtta
     3961 tgattctaaa gggaaaaaaa ttcaaaaata tcgtagtgga ttcactacta aaaaagaagc
     4021 aagagaagaa tattcgaaac tgctattgat gaaaccagaa gaattaagtg agaataagga
     4081 taaaatgacg tttaaacatt atatctttga aattttcctt ccatggtata agacacaggt
     4141 aaagctcaga acatatgaaa atcgcttacc aactattaag aagcatttct cttattttga
     4201 taaaatggct gtttccgata ttgagcctat tgatgtgcaa aattggcaac taaaattatc
     4261 aaagaagtgt aagtcttctt atgtaagagc tgttcaagga ctattttcag ttgctatgga
     4321 tcgagcaatt gtccttggga taaccaccac aaatccatct aagattattg gcaatgtgaa
     4381 aaagcaaaag tctaaaattg aattttggac aaaagaagaa tttgaaaaag taatctctct
     4441 aatttacaaa gaagattact atcaacactt tttatttatc tctctgtggt ttttatttat
     4501 gactggaatg cgaattggtg aagctactgc cattcaatgg gaagatattg attttgactc
     4561 aggagtacta accatcaaca aaacactcta ttacaagaat caatcgaatt atagatttgt
     4621 tgaaccaaaa acaaaggcga gcgttcgtca tattgtatta gataaatgca cattaactta
     4681 tttatccgaa tggaaggaag tacaacaaaa tctaattcag acagactttg taatgagcta
     4741 taacggtatt cctacccaaa aacatacact atcatatgct attgaacgct atgctaagct
     4801 ggctggtatc catcgaatca gaatccatgg gttacggcat tctcatgctt ccttgttaat
     4861 cagtatggga gaaaatccac ttatcattaa agatcgtcta ggacacgaag atattgagac
     4921 gactttagga acgtatggac atctttatcc taacagtaat ttcgaggtag ctaacaaatt
     4981 aaatggagcc atttccttta aagaagcaaa acaaaattat gatacttcac caaagaatca
     5041 atttactgta gactacttgc gaaacaaaga aatgaaaaaa gtgcaataaa agtgcagtaa
     5101 aaagataaga agagccagaa acccttggta taagaggatt tctagctctc ctacaactat
     5161 tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg
     5221 gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca
     5281 aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa
     5341 gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc
     5401 cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg
     5461 cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga
     5521 ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca
     5581 cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct
     5641 ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg
     5701 ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt
     5761 tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt
     5821 tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatc gactttgatg
     5881 atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt
     5941 agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga
     6001 accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca
     6061 acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct
     6121 tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga
     6181 cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca
     6241 atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag
     6301 tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga
     6361 tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa
     6421 ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct
     6481 aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg
     6541 ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg
     6601 ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac
     6661 tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc
     6721 aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg
     6781 ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat
     6841 atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt
     6901 ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata
     6961 ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca
     7021 tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac
     7081 gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc
     7141 aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac
     7201 aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc
     7261 catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg
     7321 atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg
     7381 agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc
     7441 gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa
     7501 gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat
     7561 atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc
     7621 ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg
     7681 gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt
     7741 ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa
     7801 tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga
     7861 tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct
     7921 gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc
     7981 tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt
     8041 gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg
     8101 ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa
     8161 atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa
     8221 atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat
     8281 gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg
     8341 acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa
     8401 caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc
     8461 acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag
     8521 tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt
     8581 gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag
     8641 agtcgt
//
LOCUS       DAZUMB010000344         5613 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.144, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000344 DAZUMB010000000
VERSION     DAZUMB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.144"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1026)
                     /locus_tag="ACG80L_003068"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="ACG80L_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACG80L_003069"
     CDS             1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACG80L_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS5025213.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="ACG80L_003070"
     CDS             1851..3005
                     /locus_tag="ACG80L_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS5025214.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="ACG80L_003071"
     CDS             3220..4188
                     /locus_tag="ACG80L_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS5025215.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="ACG80L_003072"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="ACG80L_003072"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS5025216.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="ACG80L_003073"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="ACG80L_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DAZUMB010000345         5065 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.159, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000345 DAZUMB010000000
VERSION     DAZUMB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.159"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..661
                     /locus_tag="ACG80L_003074"
     CDS             32..661
                     /locus_tag="ACG80L_003074"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5025217.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1015..2493
                     /gene="msr(C)"
                     /locus_tag="ACG80L_003075"
     CDS             1015..2493
                     /gene="msr(C)"
                     /locus_tag="ACG80L_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5025218.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2540..2986
                     /locus_tag="ACG80L_003076"
     CDS             2540..2986
                     /locus_tag="ACG80L_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5025219.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3185..3700)
                     /locus_tag="ACG80L_003077"
     CDS             complement(3185..3700)
                     /locus_tag="ACG80L_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5025220.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3817..5019
                     /locus_tag="ACG80L_003078"
     CDS             3817..5019
                     /locus_tag="ACG80L_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5025221.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1556 a          846 c         1097 g         1566 t
ORIGIN      
        1 ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt tttcaacaat
       61 tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc cacacgaaga
      121 aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata tgaaaccatg
      181 gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt tgatccgttt
      241 caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg atatgcaatg
      301 ttacgaatat ggcgaagaaa tctacaacca agcttacgaa tcagggaaga tgccaaaaga
      361 agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt caagagaaga
      421 aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca tgttagttgc
      481 gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc agttggcaga
      541 ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa taggaaaagc
      601 ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta tgttcgaata
      661 aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg tggtaaccta
      721 ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga acttttgaat
      781 aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg gagtatgtcc
      841 attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga tttagtcata
      901 gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca gatttttctg
      961 ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa acatatggaa
     1021 aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct agaactatct
     1081 attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga aaatggtgtc
     1141 ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca tgggaaaatc
     1201 caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga agcaaaggac
     1261 ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc agagcggaaa
     1321 tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa
     1381 gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga tcgactgatt
     1441 gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg ctattttcta
     1501 gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga gttttcgggg
     1561 aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct acgagaagca
     1621 gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa
     1681 gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag actggatcgg
     1741 ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca
     1801 ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat gaaacaaatc
     1861 cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat gggacaaaat
     1921 gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca gttttcttta
     1981 ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt attagaacat
     2041 atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca agtatatcag
     2101 caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat gagacaaaca
     2161 gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc tcaggaaact
     2221 ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac gattgctttg
     2281 ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt tattgatatg
     2341 gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat tttgtttact
     2401 tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat aaaagggcag
     2461 aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt tcgtgacaaa
     2521 tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt ttacaacaat
     2581 gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc atcaagatct
     2641 tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt tcattttgga
     2701 tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag taaatccaga
     2761 aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa tggtgacatg
     2821 tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat tctctgctta
     2881 tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga tcatcgttgg
     2941 cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga acactgttat
     3001 ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt ctagaaaaat
     3061 agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt tccaactgta
     3121 ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag gttctgttta
     3181 tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat tggagttcct
     3241 gctgacaaag cataattctg cgctggatca caacataacg tccaaattct tgtttcttgt
     3301 ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc agctgttgtt
     3361 ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa gcatagattt
     3421 ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc cattcctcta
     3481 caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc tcgtctttgt
     3541 ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag atctgactgt
     3601 gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa cgtggtttgg
     3661 ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta cctctcgtct
     3721 tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata ttgacatgta
     3781 aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga gaatcaaata
     3841 gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct agttgtagca
     3901 gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt aaaaacgatt
     3961 gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag ttacatgtta
     4021 gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg tttgaaaaaa
     4081 acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg attatctggg
     4141 aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc
     4201 tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa agcaatcatg
     4261 atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg
     4321 ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt ttgcggtttt
     4381 atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt aggcctttgg
     4441 gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat cgcattgtta
     4501 tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat tggctattct
     4561 gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat tgcttcgatt
     4621 ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt agcagcattt
     4681 gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc agtccttgta
     4741 ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact gttgacgacg
     4801 attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta tagttttgtc
     4861 cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga acatattggt
     4921 gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt
     4981 tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa
     5041 gctgtcctgc atcaagtcat aagaa
//
LOCUS       DAZUMB010000346         2237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.236, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000346 DAZUMB010000000
VERSION     DAZUMB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.236"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..242)
                     /locus_tag="ACG80L_003079"
     CDS             complement(<1..242)
                     /locus_tag="ACG80L_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS5025222.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFML"
     gene            335..684
                     /locus_tag="ACG80L_003080"
                     /pseudo
     CDS             335..684
                     /locus_tag="ACG80L_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(956..1246)
                     /locus_tag="ACG80L_003081"
     CDS             complement(956..1246)
                     /locus_tag="ACG80L_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5025223.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1454..2146)
                     /locus_tag="ACG80L_003082"
     CDS             complement(1454..2146)
                     /locus_tag="ACG80L_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5025224.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          631 a          423 c          333 g          850 t
ORIGIN      
        1 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
       61 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      121 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      181 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      241 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      301 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      361 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      421 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      481 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      541 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      601 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      661 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      721 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      781 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      841 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      901 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
      961 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1021 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1081 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1141 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1201 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1261 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1321 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1381 tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat ttaggtttac
     1441 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     1501 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     1561 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgagtt tctacatttg
     1621 ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc atttacagta ttttgtaatt
     1681 ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc agatgattga
     1741 tttcatcatt tttttggttg atctcatcta gtttgacatt gatttcattt tgtttgcttt
     1801 gtagttgtgc atccttgctg actaattgac tttctaagtt ttgtttttct gctgccgatt
     1861 gatttttttg gttttctaat tcatttactt ttgtttggta ttcttggaca tctgcctttg
     1921 aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt aagatctgta agcgcttgct
     1981 ctttatctgt caatgtatga tcaagctgtg tcagattctc attgattgta tcaatatctg
     2041 tctctccacc ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag ccaattagac
     2101 atagaaatgc aacgacaatc ccaccagtaa taaatttctt tttcatttgc atagctcctt
     2161 aatccaatac ttttacatgc taaaagtaag tgatctcaaa cacaatgtca agcgttttta
     2221 aattacatgt gtaattt
//
LOCUS       DAZUMB010000347         1504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.259, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000347 DAZUMB010000000
VERSION     DAZUMB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.259"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACG80L_003083"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACG80L_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACG80L_003084"
     CDS             394..477
                     /locus_tag="ACG80L_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFS5025225.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG80L_003085"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG80L_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS5025226.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAZUMB010000348         1417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.266, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000348 DAZUMB010000000
VERSION     DAZUMB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.266"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..>1417
                     /gene="aph(2'')-I"
                     /locus_tag="ACG80L_003086"
     CDS             53..>1417
                     /gene="aph(2'')-I"
                     /locus_tag="ACG80L_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFS5025227.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          596 a          103 c          244 g          474 t
ORIGIN      
        1 ttataaagat gaaatagtat atgtatatga ttatgaaaaa ggtgataaat aaatgaatat
       61 agttgaaaat gaaatatgta taagaacttt aatagatgat gattttcctt tgatgttaaa
      121 atggttaact gatgaaagag tattagaatt ttatgatggt agagataaaa aatatacatt
      181 agaatcatta aaaaaacatt atacagagcc ttgggaagat gaagttttta gagtaattat
      241 tgaatataac aatgttccta ttggatatgg acaaatatat aaaatgtatg atgagttata
      301 tactgattat cattatccaa aaactgatga gatagtctat ggtatggatc aatttatagg
      361 agagccaaat tattggagta aaggaattgg tacaagatat attaaattga tttttgaatt
      421 tttgaaaaaa gaaagaaatg ctaatgcagt tattttagac cctcataaaa ataatccaag
      481 agcaataagg gcataccaaa aatctggttt tagaattatt gaagatttgc cagaacatga
      541 attacacgag ggcaaaaaag aagattgtta tttaatggaa tatagatatg atgataatgc
      601 cacaaatgtt aaggcaatga aatatttaat tgagcattac tttgataatt tcaaagtaga
      661 tagtattgaa ataatcggta gtggttatga tagtgtggca tatttagtta ataatgaata
      721 catttttaaa acaaaattta gtactaataa gaaaaaaggt tatgcaaaag aaaaagcaat
      781 atataatttt ttaaatacaa atttagaaac taatgtaaaa attcctaata ttgaatattc
      841 gtatattagt gatgaattat ctatactagg ttataaagaa attaaaggaa cttttttaac
      901 accagaaatt tattctacta tgtcagaaga agaacaaaat ttgttaaaac gagatattgc
      961 cagtttttta agacaaatgc acggtttaga ttatacagat attagtgaat gtactattga
     1021 taataaacaa aatgtattag aagagtatat attgttgcgt gaaactattt ataatgattt
     1081 aactgatata gaaaaagatt atatagaaag ttttatggaa agactaaatg caacaacagt
     1141 ttttgagggt aaaaagtgtt tatgccataa tgattttagt tgtaatcatc tattgttaga
     1201 tggcaataat agattaactg gaataattga ttttggagat tctggaatta tagatgaata
     1261 ttgtgatttt atatacttac ttgaagatag tgaagaagaa ataggaacaa attttggaga
     1321 agatatatta agaatgtatg gaaatataga tattgagaaa gcaaaagaat atcaagatat
     1381 agttgaagaa tattatccta ttgaaactat tgtttat
//
LOCUS       DAZUMB010000349         1382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.268, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000349 DAZUMB010000000
VERSION     DAZUMB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.268"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1311
                     /locus_tag="ACG80L_003087"
     CDS             <1..1311
                     /locus_tag="ACG80L_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFS5025228.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAZUMB010000350         1350 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.271, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000350 DAZUMB010000000
VERSION     DAZUMB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.271"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACG80L_003088"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACG80L_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACG80L_003089"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACG80L_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS5025229.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAZUMB010000351         1196 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.284, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000351 DAZUMB010000000
VERSION     DAZUMB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.284"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..>1196
                     /gene="vanY"
                     /locus_tag="ACG80L_003090"
     CDS             259..>1196
                     /gene="vanY"
                     /locus_tag="ACG80L_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5025230.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          428 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaataa
//
LOCUS       DAZUMB010000352          942 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5109
            SAMEA115707498-rid22580363.guided.305, whole genome shotgun
            sequence.
ACCESSION   DAZUMB010000352 DAZUMB010000000
VERSION     DAZUMB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707498
            Sequence Read Archive: ERR13197227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288045.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288045.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:05:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 52 of 95
            Pseudo Genes (internal stop)      :: 27 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707498-rid22580363.guided.305"
                     /isolate="VRE5109"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..775
                     /gene="aac(6')"
                     /locus_tag="ACG80L_003091"
     CDS             227..775
                     /gene="aac(6')"
                     /locus_tag="ACG80L_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5025231.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          331 a          141 c          201 g          269 t
ORIGIN      
        1 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
       61 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
      121 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
      181 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
      241 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
      301 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
      361 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
      421 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
      481 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
      541 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
      601 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
      661 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
      721 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
      781 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
      841 cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta
      901 ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aa
//
